CN105699611A - Quality grade division method for fresh and raw milk - Google Patents
Quality grade division method for fresh and raw milk Download PDFInfo
- Publication number
- CN105699611A CN105699611A CN201610040689.XA CN201610040689A CN105699611A CN 105699611 A CN105699611 A CN 105699611A CN 201610040689 A CN201610040689 A CN 201610040689A CN 105699611 A CN105699611 A CN 105699611A
- Authority
- CN
- China
- Prior art keywords
- milk
- fresh
- fresh milk
- grade
- plate count
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/04—Dairy products
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/04—Dairy products
- G01N33/06—Determining fat content, e.g. by butyrometer
Abstract
The invention provides a quality grade division method for fresh and raw milk. The method comprises the following steps: (1) collecting fresh and raw milk materials, and carrying out component content analysis on four indexes, namely lactoprotein, milk fat, somatic cell count and total number of bacterial colonies of the fresh and raw milk; (2) dividing the fresh and raw milk into a super-superior grade, a superior grade, an excellent grade and a qualified grade according to the component contents reflecting the four indexes, namely the lactoprotein, the milk fat, the somatic cell count and the total number of bacterial colonies of the fresh and raw milk. Compared with the prior art, the invention provides a set of scientific and objective division method of quality grades of the fresh and raw milk against the problems that customers lacks the right to know the quality grades of the fresh and raw milk and the quality characteristic of the fresh and raw milk changes.
Description
Technical field
The present invention relates to the stage division of a kind of fresh milk credit rating, belong to milk quality assessment technique field, be exclusively used in the division methods of fresh milk credit rating。
Background technology
In recent years, milk cattle cultivating comparative effectiveness glides increasingly and dairy farmer's quantity gradually decreases, in order to increase milk cattle cultivating benefit and improve dairy farmer and cultivate confidence, fresh milk is carried out classification to realize the division of different quality fresh milk quality, allow consumer take a box milk it is known that it is to make of the fresh milk of what grade, allow the relieved consumption of consumer, transparent consumption, adapting to the needs in quality control and market, thus reaching high quality and favourable price, promoting China milk industry to develop in a healthy way has important meaning。
Summary of the invention
The core index of fresh milk is butterfat percnetage, protein ratio, total plate count and somatic number, and wherein butter oil and lactoprotein are the Nutrition quality indicator of milk, and total plate count is environmental health index, and somatic number is milk cow health status index。By quantifying the height of 4 indexs just apparent fresh milk quality of energy。
The present invention is after technical specification is determined, by to 2013 to 2014 years, annual four season, national 11 provinces and cities 270 batches, 270 cattle farms, 4 indexs of fresh milk sample, 1180 big data verifications test, obtain the accreditation being engaged in the cultivation of milk industry and working research personnel, achieve very good effect。
It is an object of the invention to provide a kind of fresh milk quality grade compartmentalization method, the right to know of fresh milk credit rating is lacked and fresh milk mass property variation issue by it for consumer, it is proposed to the stage division of the objective fresh milk credit rating of a set of science。
For reaching object defined above, the fresh milk quality grade compartmentalization method of the present invention, it comprises the steps:
(1) gather fresh milk material, it is carried out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count；
(2) according to the reflection lactoprotein of fresh milk mass property, butter oil, somatic number and 4 indexs of total plate count component content fresh milk is divided into special top grade, top grade, excellent level and regular grade。
As one preferred embodiment of invention, each index content of the fresh milk of described special top grade is: butterfat percnetage >=3.3%, protein ratio >=3.1%, somatic number≤400,000/mL, total plate count≤100,000 CFU/mL；
Each index content of the fresh milk of described top grade is: butterfat percnetage >=3.2%, protein ratio >=3.0%, somatic number≤750,000/mL, total plate count≤300,000 CFU/mL；
Each index content of the fresh milk of described excellent level is: butterfat percnetage >=3.1%, protein ratio >=2.9%, somatic number≤900,000/mL, total plate count≤500,000 CFU/mL；
Each index content of the fresh milk of described regular grade is: butterfat percnetage >=3.1%, protein ratio >=2.8%, somatic number≤1,000,000/mL, total plate count≤2,000,000 CFU/mL。
Further, the present invention provides the acquisition method of a kind of fresh milk, first fresh milk material is carried out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count by it, if the content of 4 indexs is within the scope of A, is then acquired as special top grade milk supply；If the content of 4 indexs is within the scope of B, then it is acquired as top grade milk supply；If the content of 4 indexs is within the scope of C, then it is acquired as excellent level milk supply, if the content of 4 indexs is within the scope of D, is then acquired as regular grade milk supply。
As one preferred embodiment of the present invention, described A ranges for: butterfat percnetage >=3.3%, protein ratio >=3.1%, somatic number≤400,000/mL, total plate count≤100,000 CFU/mL；
Described B ranges for: butterfat percnetage >=3.2%, protein ratio >=3.0%, somatic number≤750,000/mL, total plate count≤300,000 CFU/mL；
Described C ranges for: butterfat percnetage >=3.1%, protein ratio >=2.9%, somatic number≤900,000/mL, total plate count≤500,000 CFU/mL；
Described D ranges for: butterfat percnetage >=3.1%, protein ratio >=2.8%, somatic number≤1,000,000/mL, total plate count≤2,000,000 CFU/mL
Beneficial effects of the present invention:
(1) this standard has filled up the blank of domestic fresh milk credit rating standard, has been greatly facilitated the development of cow estate；In Cow product process, no matter from genetic breeding or from diet management and milking process, all more or less there are differences, thus having influence on the quality of fresh milk, so the fresh milk grade scale of other country can not be copied slavishly；This grade scale adopts the core index of reflection fresh milk quality, in conjunction with substantial amounts of sample research work, it is possible to China's fresh milk quality comparatively scientifically carries out the standardization of classification。
(2), in 4 ranks that this fresh milk quality classification standard includes, special top grade reaches European Union's fresh milk " guaranteed milk " standard；Top grade has reached U.S.'s fresh milk " guaranteed milk " standard；Excellent level is the average level of fresh milk in the world；Regular grade is under China's reality cultivating condition, the acceptance condition of fresh milk quality；Grade scale is conducive to the goods that different fresh milks are processed into by consumer to select, and makes enterprise's high quality and favourable price purchase fresh milk simultaneously, produces the milk product of high-quality, accomplish " fixing the price according to the quality "。
(3), after fresh milk grade scale is formulated, in the fresh milk risk assessment work of national 11 provinces and cities, acquire substantial amounts of sample and carry out classification proving test, obtaining the accreditation being engaged in the cultivation of milk industry and working research personnel, achieve very good effect。
Detailed description of the invention
Understandable for enabling the above-mentioned purpose of the present invention, feature and advantage to become apparent from, below the present invention is further detailed explanation。
" embodiment " or " embodiment " referred to herein refers to the special characteristic, structure or the characteristic that may be included at least one implementation of the present invention。Different local in this manual " in one embodiment " occurred not refer both to same embodiment, neither be independent or selective and that other embodiments are mutually exclusive embodiment。
Fresh milk quality grade compartmentalization method of the present invention includes:
(1) gather fresh milk material, it is carried out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count；
(2) according to the reflection lactoprotein of fresh milk mass property, butter oil, somatic number and 4 indexs of total plate count component content fresh milk is divided into special top grade, top grade, excellent level and regular grade。Wherein, the fresh milk grade scale such as table 1 below of the present invention:
Table 1 fresh milk credit rating sorting parameter of the present invention
The following stage division describing the present invention for milk, it is necessary to explanation, the milk supply of the present invention is not limited to milk。
Embodiment 1
The present invention comprises the steps:
(1) collecting milk raw material, carries out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count to it。
(2) after tested, to be 3.3% (mass percent), protein ratio be 3.2% (mass percent) of the butterfat percnetage in milk, somatic number are 400,000/mL, total plate count is 100,000 CFU/mL。Then this milk can as special top grade milk supply。This spy's top grade milk supply reaches European Union's fresh milk " guaranteed milk " standard。
Embodiment 2
The present invention comprises the steps:
(1) collecting milk raw material, carries out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count to it。
(2) after tested, to be 3.2% (mass percent), protein ratio be 3.0% (mass percent) of the butterfat percnetage in milk, somatic number are 750,000/mL, total plate count is 300,000 CFU/mL。Then this milk can as top grade milk supply。This top grade has reached U.S.'s fresh milk " guaranteed milk " standard。
Embodiment 3
The present invention comprises the steps:
(1) collecting milk raw material, carries out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count to it。
(2) after tested, to be 3.1% (mass percent), protein ratio be 2.9% (mass percent) of the butterfat percnetage in milk, somatic number are 900,000/mL, total plate count is 500,000 CFU/mL。Then this milk can as excellent level milk supply。This excellent level is the average level of fresh milk in the world。
Embodiment 4
The present invention comprises the steps:
(1) collecting milk raw material, carries out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count to it。
(2) after tested, to be 3.1% (mass percent), protein ratio be 2.8% (mass percent) of the butterfat percnetage in milk, somatic number are 1,000,000/mL, total plate count is 2,000,000 CFU/mL。Then this milk can as regular grade milk supply。This regular grade is under China's reality cultivating condition, the acceptance condition of fresh milk quality。
Embodiment 5
In this embodiment, described somatic number is 650,000/mL, and described total plate count is 250,000 CFU/mL, and other are identical with embodiment 1。Then this milk can divide as top grade milk supply。
It should be noted that, the fresh milk quality grade compartmentalization method of the present invention, by fresh milk material being carried out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count, if the content of 4 indexs is entirely in the scope of special top grade, then this milk supply is just acquired as special top grade milk supply。If one of them index in a rank is not up to standard, the index not up to standard further according to this re-starts classification。Such as, the testing result of fresh milk, if at least the 1 of milk supply index is within the scope of top grade, other indexs are within the scope of special top grade, then this milk supply is divided into top grade milk supply；If at least the 1 of milk supply index is within the scope of excellent level, other indexs are within the scope of special top grade, then this milk supply is divided into excellent level milk supply；If at least the 1 of milk supply index is within the scope of regular grade, other indexs are within the scope of special top grade, then this milk supply is divided into regular grade milk supply。By that analogy, other are in like manner。
Butter oil, lactoprotein, somatic number and total plate count assay method
(1) fresh milk butter oil, lactoprotein assay method
Butter oil and lactoprotein in the present invention pass through MilkoScanFT120 milk analysis-e/or determining。Concrete operations code is as follows:
1, start:
Booting computer；Opening instrument power source, switch is positioned at after instrument above power line。Click the operating system icon on screen, enter 120 milk analyser operation interfaces。Treat " ready " printed words that the display of screen lower right is green, it was shown that instrument preheating terminates, and can continue operation。
2, instrument is cleaned:
By cleaning button or selecting " clean " from " Analysis " menu。First with S-6060 return to zero liquid clean, then with S-470 cleanout fluid clean, then with S-6060 return to zero liquid clean。
3, zeroing:
The liquid that returned to zero by S-6060 is placed under suction pipe, strikes " Zero-set " button or press Ctrl+Z key in button panel, if instrument zeroing liquid tests 5 results in the scope of programming, instrument will complete zeroing。If gone beyond the scope, must cleaning instrument again, rezeroing accepts。
4, test analysis:
Select sample program；
It is placed under aspirator after mixing sample；
Start button in the version of selection face starts to analyze, or presses F9, it is also possible to start by the start button of instrument sampler upper right side。
The meansigma methods of measurement result and all the components shows in result window。
5, clean:
Instrument is carried out by cleaning step time after end-of-job according to start。
6, shutdown: close software and instrument power source, shut down computer。Cleaning, makes a record。
(2) assay method of fresh milk somatic number
The mensuration of the somatic number in the present invention is by Fossmatic5000 somatic cell analysis-e/or determining, and concrete operation step is as follows:
1, start
Preheating milk sample is to 37-42 DEG C
First connecting Fossmatic5000 instrument power source, switch is positioned at after instrument above power line。
Then start computer, press Start key, run IMTS4000 systems soft ware。
2, prepare before test
After opening software, systemmanager window occurs, select worksessions option, create working group。
Three inspections were carried out before test sample:
(1) " blank " checks (blind):
Prepare appropriate amount of deionized water to be placed under sampler；
The manual measurement icon on the left of the page is selected under operation interface；
Click " setting " to occur arranging interface, under " Sample " option, select " blind "；
Check that what adopt is same sample if blank, on interface selected " samebottle " this；
Select interface bottom-right " store ", click " measure ", start blank inspection。
(2) " repeatability " checks (repeat):
Prepare appropriate milk sample to be placed under sampler；
The manual measurement icon on the left of the page is selected under operation interface；
Click " setting " to occur arranging interface, under " Sample " option, select " repeat "；
Select interface bottom-right " store ", click " measure ", start iterative testing。
(3) " transmission error " checks (carryover)
Preparing appropriate milk sample and be placed under sampler, sample is by a milk sample, and two portions of buffer are placed on specimen holder；
The manual measurement icon on the left of the page is selected under operation interface；
Click " setting " to occur arranging interface, under " Sample " option, select " carryover "；
Select interface bottom-right " store ", click " measure ", start to transmit error-detecting。
3, test sample
When selecting manual measurement, click the manual measurement icon on the left of interface, then under setting option, corresponding process of measurement (FM, MSC, COMBI) is set；Click measurement；
When selecting automatically to measure, also to select corresponding process of measurement (FM, MSC, COMBI)。Click continuewithBatch (Batch that you have set up)。
4, shutdown
After selecting NormalStop to make instrument enter halted state under the operation interface of S4000 software, press Alt+F4 key and exit software, shut down computer, close instrument power source。Cleaning, makes a record。
Note: 1. forbid to directly initiate computer when instrument is not keyed up and run S4000 software。2. check that whether waste liquid barrel is full, changes in time。
(3) mensuration of fresh milk total plate count
In the present invention, fresh milk total plate count assay method is measured by culture method, and concrete operation step is as follows:
Described in fresh milk total plate count assay method of the present invention use equipment and material be: except Microbiological Lab's conventional sterilant and culture device, other equipment and material are as follows:
Constant incubator: 36 DEG C ± 1 DEG C, 30 DEG C ± 1 DEG C。
Refrigerator: 2 DEG C～5 DEG C。
Constant water bath box: 46 DEG C ± 1 DEG C。
Balance: sensibility reciprocal is 0.1g。
Homogenizer。
Agitator。
Aseptic straw: 1mL (tool 0.01mL scale), 10mL (tool 0.1mL scale) or micropipettor and suction nozzle。
Aseptic conical flask: capacity 250mL, 500mL。
Sterile petri dish: diameter 90mm。
PH meter or pH color comparison tube or accurate pH test paper。
Magnifier is or/and colonometer。
Described in fresh milk total plate count assay method of the present invention use culture medium and reagent be:
Plate count agar culture medium。
Phosphate buffer。
Physiological saline solution。
The testing procedure of fresh milk total plate count of the present invention includes:
Sample, takes 25g (ml) sample, is subsequently adding 225ml diluent, homogenizing。
10 times of serial dilutions。
Select the even liquid of sample of 2-3 acceptable diluent degree, respectively take 1ml and be separately added in sterile petri dish。
Every ware adds 15ml-20ml plate count agar culture medium, mixing。
Cultivate。
Count each flat-plate bacterial colony number。
Counting total plate count。
The concrete operation step of fresh milk total plate count of the present invention is as follows:
Step one, sample dilution, comprising:
S1, fluid sample: draw 25mL sample with aseptic straw and put in the aseptic conical flask (in bottle preset an appropriate number of sterile glass beads) filling 225mL phosphate buffer or normal saline, fully mix, make the even liquid of sample of 1:10。
S2, use 1mL aseptic straw or micropipettor draw the even liquid 1mL of 1:10 sample, slowly note along tube wall and (notice that suction pipe or tips do not touch dilution liquid level) in the sterile test tube filling 9mL diluent, jolting test tube or use 1 aseptic straw instead and repeatedly blow and beat and make its mix homogeneously, makes the even liquid of sample of 1:100。
S3, by S2, operation sequence, prepare 10 times of even liquid of series of diluted samples。Often it is incremented by dilution once, uses 1 1mL aseptic straw or suction nozzle instead。
S4, the basis estimation to sample contamination situation, select the even liquid of sample (fluid sample can include stock solution) of 2～3 acceptable diluent degree, when carrying out 10 times and being incremented by dilution, drawing the even liquid of 1mL sample in sterilized petri dishes, each dilution factor makees two plates。Meanwhile, draw respectively in 1mL solution dilution blanks two sterilized petri dishes of addition and make blank。
S5, in time 15mL～20mL is cooled to plate count agar culture medium (can be positioned in 46 DEG C ± 1 DEG C constant water bath box and the be incubated) pour plate of 46 DEG C, and rotates plate and make its mix homogeneously。
Step 2, cultivation, comprising:
A1, after agar solidification, by Flat plate turnover, 36 DEG C ± 1 DEG C cultivate 48h ± 2h。Aquatic products 30 DEG C ± 1 DEG C cultivate 72h ± 3h。
If A2 sample is likely to containing, when filling the air the bacterium colony of growth at agar media surface, a thin layer agar culture medium (about 4mL) being covered by agar surface after solidification, overturn flat board after solidification, cultivate by 6.2.1 condition。
Step 3, colony counting, can detect by an unaided eye, and if desired with magnifier or colonometer, records extension rate and corresponding colony counts。Colony counting represents with colony-forming units (colony-formingunits, CFU)。It comprises the steps:
B1, choose clump count between 30CFU～300CFU, without spreading the plate count total plate count of colony growth。The concrete clump count of flat board record lower than 30CFU, recordable more than 300CFU is how can not count。Each dilution clump count should adopt the average of two flat boards。
When B2, one of them flat board have larger piece shape colony growth, then should not adopt, and should using the flat board without lamellar colony growth as this dilution clump count；If lamellar bacterium colony is less than the half of flat board, and in all the other half, bacterium colony distribution is very uniform, is multiplied by 2, represents a flat-plate bacterial colony number after can calculating half flat board。
B3, when flat board occurs between bacterium colony the chain-like growth without obvious boundary line, then using every strand as a colony counting。
Step 4, total plate count computational methods, comprising:
If the clump count on C1 only one of which dilution factor flat board is in suitable count range, calculates the meansigma methods of two flat-plate bacterial colony numbers, then meansigma methods is multiplied by corresponding extension rate, as total plate count result in every g (mL) sample。
If C2 has the flat-plate bacterial colony number of two serial dilution degree in suitable count range, calculate by formula (1):
N=∑ C/ (n1+0.1n2)d…………………………………(1)
In formula:
Clump count in N sample；
Σ C flat board (flat board containing optimum range clump count) clump count sum；
N1 the first dilution factor (low extension rate) flat board number；
N2 the second dilution factor (highly diluted multiple) flat board number；
D dilution gfactor (the first dilution factor)。
If clump count is all higher than 300CFU on all dilution flat boards of C3, then the flat board that dilution factor is the highest being counted, other flat boards are recordable for how counting, and result is multiplied by most highly diluted multiple by average colony number and calculates。
If all dilution flat-plate bacterial colony numbers of C4 are respectively less than 30CFU, then should be multiplied by extension rate by the average colony number that dilution factor is minimum and calculate。
If all dilution factors of C5 (include fluid sample stock solution) flat board is all without colony growth, then calculate being multiplied by minimum extension rate less than 1。
If all dilution flat-plate bacterial colony numbers of C6 are not all between 30CFU～300CFU, when a portion is less than 30CFU or more than 300CFU, then it is multiplied by extension rate with the average colony number closest to 30CFU or 300CFU and calculates。
To sum up, when clump count is less than 100CFU, by " rounding up " principle revision of the convention, report with integer。
When clump count is more than or equal to 100CFU, the 3rd bit digital adopts after " rounding up " principle revision of the convention, takes front 2 bit digital, after by 0 replacement figure place；Also can represent by the exponential form of 10, by after " rounding up " principle revision of the convention, adopt two position effective digitals。
If cannot count for spreading bacterium colony on all flat boards, then report bacterium colony spreads。
If there being colony growth on blank, then this time testing result is invalid。
Sampling of weighing is reported in units of CFU/g, and volume sampling is reported in units of CFU/mL。
The fresh milk quality grade compartmentalization method of the present invention, has the advantage that
(1) it is the classification standard of the fresh milk being different from the milk industry developed countries and regions such as the U.S., European Union, and this method has filled up the blank of domestic fresh milk credit rating standard, has been greatly facilitated the development of local cow estate。Owing to, in every country cow estate, no matter from genetic breeding or from diet management and milking process, all more or less there are differences, thus having influence on the quality of fresh milk, so the fresh milk grade scale of other country can not be copied slavishly；This grade scale adopts the core index of reflection fresh milk quality, in conjunction with substantial amounts of sample research work, it is possible to China's fresh milk quality comparatively scientifically carries out the standardization of classification。
(2), in 4 ranks that this fresh milk quality classification standard includes, special top grade reaches European Union's fresh milk " guaranteed milk " standard；Top grade has reached U.S.'s fresh milk " guaranteed milk " standard；Excellent level is the average level of fresh milk in the world；Regular grade is under China's reality cultivating condition, the acceptance condition of fresh milk quality；Grade scale is conducive to the goods that different fresh milks are processed into by consumer to select, and makes enterprise's high quality and favourable price purchase fresh milk simultaneously, produces the milk product of high-quality, accomplish " fixing the price according to the quality "。
(3), after fresh milk grade scale is formulated, 2013 years, to 2014 years, to 16 provinces, the whole nation, included milk owner and produce province and have the representational province of region。Wherein, 5 provinces of northern area, including: Heilungkiang, Tianjin, Hebei, Shandong, Beijing；Province, 4, the Northwest, including: Xinjiang, the Inner Mongol, Shaanxi, Gansu；Province, 5, southern area, including: Jiangsu, Shanghai, Fujian, Guangdong, Guangxi；Sichuan, 2 provinces, Structure Dynamics In The Qinghai-tibet Region, province, 2, Chongqing。Annual 4 season, 270 270 batches, cattle farm, 4 indexs of fresh milk sample, 1180 big data verification tests, result shows, 1080 batches of fresh milks, Fresh Milk: 11.4% special top grade, 18.3% top grade, 17.5% excellent level, 15.3% good level, 37.5% defective；Fresh Lac caprae seu ovis: 9% special top grade, 6% top grade, 3% excellent level, 19% good level, 63% defective；Fresh buffalo's milk: 30% special top grade, 10% top grade, 0 excellent level, 22.5% good level, 37.5% defective (table 2), refers to table 2 below, and it is 2013 annual-2014 annual fresh milk classification results。
Table 2 is 2013 annual-2014 annual fresh milk classification results
The grade scale of the present invention meets China's fresh milk quality safety present situation, it is possible to realize high quality and favourable price, obtains the accreditation being engaged in the cultivation of milk industry and working research personnel, achieves very good effect。
Described above has fully disclosed the specific embodiment of the present invention。It is pointed out that any change that the specific embodiment of the present invention done by one skilled in the art scope all without departing from claims of the present invention。Correspondingly, the scope of the claims of the present invention is also not limited only to previous embodiment。
Claims (4)
1. a fresh milk quality grade compartmentalization method, it is characterised in that: comprising:
(1) gather fresh milk material, it is carried out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count；
(2) according to the reflection lactoprotein of fresh milk mass property, butter oil, somatic number and 4 indexs of total plate count component content fresh milk is divided into special top grade, top grade, excellent level and regular grade。
2. fresh milk quality grade compartmentalization method according to claim 1, it is characterised in that:
Each index content of the fresh milk of described special top grade is: butterfat percnetage >=3.3%, protein ratio >=3.1%, somatic number≤400,000/mL, total plate count≤100,000 CFU/mL；
Each index content of the fresh milk of described top grade is: butterfat percnetage >=3.2%, protein ratio >=3.0%, somatic number≤750,000/mL, total plate count≤300,000 CFU/mL；
Each index content of the fresh milk of described excellent level is: butterfat percnetage >=3.1%, protein ratio >=2.9%, somatic number≤900,000/mL, total plate count≤500,000 CFU/mL；
Each index content of the fresh milk of described regular grade is: butterfat percnetage >=3.1%, protein ratio >=2.8%, somatic number≤1,000,000/mL, total plate count≤2,000,000 CFU/mL。
3. the acquisition method of a fresh milk, it is characterised in that: first fresh milk material is carried out lactoprotein, butter oil, somatic number and 4 index components content analysis of total plate count, if the content of 4 indexs is within the scope of A, is then acquired as special top grade milk supply；If the content of 4 indexs is within the scope of B, then it is acquired as top grade milk supply；If the content of 4 indexs is within the scope of C, then it is acquired as excellent level milk supply, if the content of 4 indexs is within the scope of D, is then acquired as regular grade milk supply。
4. the acquisition method of fresh milk according to claim 3, it is characterised in that:
Described A ranges for: butterfat percnetage >=3.3%, protein ratio >=3.1%, somatic number≤400,000/mL, total plate count≤100,000 CFU/mL；
Described B ranges for: butterfat percnetage >=3.2%, protein ratio >=3.0%, somatic number≤750,000/mL, total plate count≤300,000 CFU/mL；
Described C ranges for: butterfat percnetage >=3.1%, protein ratio >=2.9%, somatic number≤900,000/mL, total plate count≤500,000 CFU/mL；
Described D ranges for: butterfat percnetage >=3.1%, protein ratio >=2.8%, somatic number≤1,000,000/mL, total plate count≤2,000,000 CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610040689.XA CN105699611A (en) | 2016-01-21 | 2016-01-21 | Quality grade division method for fresh and raw milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610040689.XA CN105699611A (en) | 2016-01-21 | 2016-01-21 | Quality grade division method for fresh and raw milk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105699611A true CN105699611A (en) | 2016-06-22 |
Family
ID=56226715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610040689.XA Pending CN105699611A (en) | 2016-01-21 | 2016-01-21 | Quality grade division method for fresh and raw milk |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105699611A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271621A (en) * | 2017-07-18 | 2017-10-20 | 东北农业大学 | A kind of method of utilization cow's milk detection cow mammary gland synthesis lactoprotein and butter oil ability |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999456A (en) * | 2010-08-31 | 2011-04-06 | 华南理工大学 | Method for controlling total number of bacteria in fresh milk |
| CN102590460A (en) * | 2012-03-05 | 2012-07-18 | 昆明理工大学 | Method for rating of raw milk quality |
| CN105572313A (en) * | 2015-10-12 | 2016-05-11 | 中国农业科学院北京畜牧兽医研究所 | Method for grading quality of fresh and raw milk |
-
2016
- 2016-01-21 CN CN201610040689.XA patent/CN105699611A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999456A (en) * | 2010-08-31 | 2011-04-06 | 华南理工大学 | Method for controlling total number of bacteria in fresh milk |
| CN102590460A (en) * | 2012-03-05 | 2012-07-18 | 昆明理工大学 | Method for rating of raw milk quality |
| CN105572313A (en) * | 2015-10-12 | 2016-05-11 | 中国农业科学院北京畜牧兽医研究所 | Method for grading quality of fresh and raw milk |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271621A (en) * | 2017-07-18 | 2017-10-20 | 东北农业大学 | A kind of method of utilization cow's milk detection cow mammary gland synthesis lactoprotein and butter oil ability |
| CN107271621B (en) * | 2017-07-18 | 2019-06-21 | 东北农业大学 | A method of utilizing cow's milk detection cow mammary gland synthesis lactoprotein and butter oil ability |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bakst et al. | Techniques for semen evaluation, semen storage, and fertility determination | |
| CN101542263A (en) | Device for automatically adjusting the bacterial inoculum level of a sample | |
| CA2753161C (en) | A device for measuring light scattering and turbity in biological samples and methods of use thereof | |
| US20110151503A1 (en) | Device and method for bacteriological testing on plasma | |
| KR20190121351A (en) | Sample addition system automatically adds sputasol | |
| Niemelä | Uncertainty of quantitative determinations derived by cultivation of microorganisms | |
| Berry et al. | Use of the DeLaval cell counter (DCC) on goats' milk | |
| CN105699611A (en) | Quality grade division method for fresh and raw milk | |
| Baron et al. | Clinical utility of an automated instrument for gram staining single slides | |
| CN113571128A (en) | Method for establishing reference threshold for detecting macro genomics pathogens | |
| CN105572313B (en) | A kind of fresh milk quality grade compartmentalization method | |
| Jacobs et al. | Accuracy and precision of quantitative calibrated loops in transfer of bronchoalveolar lavage fluid | |
| CN203811612U (en) | Automatic analyzer for weighing and quantitative dilution of excrement | |
| CN105572313A (en) | Method for grading quality of fresh and raw milk | |
| CN101029332A (en) | Bacterium total testing fluid and its testing bottle | |
| CN105606550A (en) | Method for quickly measuring mercury content of edible food packing material | |
| CN207366563U (en) | A kind of classification of compatible leucocyte five and leucocyte three divide the cellanalyzer of group | |
| CN108490122A (en) | A kind of pit method for evaluating quality | |
| CN103645130B (en) | Visible component High-throughput quantitative analysis method in human or animal's excretion thing | |
| CN109777856A (en) | A kind of detection method of 1,000,000,000,000 live spores per gram Bacillus subtillis original powder brood cell's number living | |
| CN102854165A (en) | Method for rapidly determining number of bacterial in lactic acid bacillus subtilis production liquid culture | |
| CN116837142B (en) | Break point cluster zone Abelson leukemia virus calibrator and preparation method and application thereof | |
| CN1587975A (en) | Method for determining milk somatic cell number | |
| CN110272973A (en) | ATP bioluminescence lgCB-lgIBThe method that calibration curve method detects antibiotic plastic bacteria resistance energy | |
| CN108333132A (en) | A kind of bio-toxicity detection method of coal chemical industrial waste water |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160622 |
|
| WD01 | Invention patent application deemed withdrawn after publication |