CN108333132A - A kind of bio-toxicity detection method of coal chemical industrial waste water - Google Patents
A kind of bio-toxicity detection method of coal chemical industrial waste water Download PDFInfo
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- CN108333132A CN108333132A CN201810154432.6A CN201810154432A CN108333132A CN 108333132 A CN108333132 A CN 108333132A CN 201810154432 A CN201810154432 A CN 201810154432A CN 108333132 A CN108333132 A CN 108333132A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
Abstract
A kind of bio-toxicity detection method of coal chemical industrial waste water, belongs to bio-toxicity detection technique field, solves the problems, such as that existing water quality biological detection method of toxicity is not suitable for carrying out bio-toxicity detection to coal chemical industrial waste water.The bio-toxicity detection method is using tetrahymena as toxotest species.Since tetrahymena is prevalent in coal chemical industrial waste water, tetrahymena has certain adaptability to bio-toxicity substance, and susceptibility will not be excessively high.Therefore, the bio-toxicity detection method of coal chemical industrial waste water of the present invention is suitable for being detected coal chemical industrial waste water.On the other hand, compared with green alga, Daphnia magna or zebra fish, tetrahymena has the advantages that be easy to culture and the breeding cycle is short.Therefore, compared with the existing water quality biological detection method of toxicity based on green alga, Daphnia magna or zebra fish, the detection cycle of the bio-toxicity detection method of coal chemical industrial waste water of the present invention is shorter, is easy to be popularized and promoted in the bio-toxicity detection field of coal chemical industrial waste water.
Description
Technical field
The present invention relates to a kind of bio-toxicity detection methods of water quality, belong to bio-toxicity detection technique field.
Background technology
Test species used by existing water quality biological detection method of toxicity be usually photogen, green alga, Daphnia magna or
Zebra fish.Among these, the water quality biological detection method of toxicity based on photogen is because photogen is to the susceptibility of bio-toxicity substance
Detection that is excessively high and not being suitable for the higher coal chemical industrial waste water of toxic equivalent.And using based on green alga, Daphnia magna or zebra fish
Bio-toxicity of the water quality biological detection method of toxicity to detect coal chemical industrial waste water when, all there is a problem of that detection cycle is long,
This severely limits these three water quality biological detection method of toxicity coal chemical industrial waste water bio-toxicity detection field application.
Invention content
The present invention is to solve existing water quality biological detection method of toxicity not being suitable for carrying out biology poison to coal chemical industrial waste water
Property detection the problem of, it is proposed that a kind of bio-toxicity detection method of coal chemical industrial waste water.
The bio-toxicity detection method of coal chemical industrial waste water of the present invention is using tetrahymena as toxotest species.
As preferably, the bio-toxicity detection method of coal chemical industrial waste water of the present invention includes：
Step 1: culture tetrahymena, obtains tetrahymena solution；
Step 2: calculating the tetrahymena density of tetrahymena solution, and judge whether tetrahymena density is less than 5 × 104cell/
Ml executes step 1 when judging result is to be, otherwise, is diluted the tetrahymena solution of predetermined volume using aseptic culture fluid
10 times, obtain tetrahymena test fluid；
Step 3: preparing control group, experimental group, the first blank group and the second blank group；
Control group be tetrahymena test fluid, experimental group be tetrahymena test fluid and water sample to be detected mixed solution, first
Blank group is aseptic culture fluid, and the second blank group is water sample to be detected；
Step 4: culture control group, experimental group, the first blank group and the second blank group；
Step 5: according to cultured control group, the absorbance of experimental group, the first blank group and the second blank group, calculate
Half-inhibition concentration of the water sample to be detected to tetrahymena；
Step 6: according to the half-inhibition concentration, the toxic equivalent of water sample to be detected is calculated.
As preferably, the detailed process of step 1 is：
Under the conditions of 120 DEG C, sterilizing in 20 minutes is carried out to culture medium；
In an aseptic environment, culture medium is cooled down；
In an aseptic environment, tetrahymena inoculation is carried out to culture medium；
Under sterile, 25 DEG C of environment, culture medium is cultivated；
Culture medium is rocked 2~3 times daily, until tetrahymena grows to logarithmic phase；
Culture is enlarged to tetrahymena, obtains the tetrahymena solution of logarithmic phase.
As preferably, in step 1,
Tetrahymena is tetrahymena；
Culture medium is PPYS culture mediums；
It is sterilized to culture medium using autoclave；
Culture medium is placed on super-clean bench cooling；
Culture medium is placed in constant incubator and is cultivated.
As preferably, step 2 calculates the tetrahymena density of tetrahymena solution based on blood counting chamber and microscope.
As preferably, in step 3,
Coal chemical industrial waste water is filtered using filter membrane, obtains water sample to be detected；
Control group, experimental group, the first blank group and the second blank group are held using experiment orifice plate.
As preferably, in step 3, control group, experimental group, the first blank group and the second blank group are placed in
Aseptic culture fluid is added dropwise in the fringe region of experiment orifice plate in the intermediate region for testing orifice plate.
As preferably, in step 4, control group, experimental group, the first blank group and the second blank group culture side
Formula is：It is sterile, 25 DEG C under the conditions of, by control group, experimental group, the first blank group and the second blank group secretly set exposure 24 hours.
As preferably, step 5 first uses microplate reader to measure control group, experimental group, the first blank group and second simultaneously
Absorbance of the blank group at 490nm obtains more parts of inhibition concentration data further according to inhibition concentration calculation formula；
Inhibition concentration calculation formula is：
In formula, μ is inhibition concentration, ODCIn order to control in group sample absorbance, ODBCFor the suction of sample in the first blank group
Luminosity, ODTFor the absorbance of sample in experimental group, ODBTFor the absorbance of sample in the second blank group；
By being fitted to more parts of inhibition concentration data, amount effect curve is obtained, and according to the amount effect curve, obtain half
Inhibition concentration.
As preferably, step 6 is calculated to be detected according to toxic equivalent calculation formula and half-inhibition concentration
The toxic equivalent of water sample；
Toxic equivalent calculation formula is TU=100%/EC50, and in formula, TU is toxic equivalent, and EC50 is that half inhibits dense
Degree.
The bio-toxicity detection method of coal chemical industrial waste water of the present invention is using tetrahymena as toxotest species.By
It is prevalent in coal chemical industrial waste water in tetrahymena, tetrahymena has certain adaptability to bio-toxicity substance, and susceptibility is not
It can be excessively high.Therefore, the bio-toxicity detection method of coal chemical industrial waste water of the present invention is suitable for examining coal chemical industrial waste water
It surveys.On the other hand, compared with green alga, Daphnia magna or zebra fish, tetrahymena has the advantages that be easy to culture and the breeding cycle is short.Cause
This, compared with the existing water quality biological detection method of toxicity based on green alga, Daphnia magna or zebra fish, coal chemical industry of the present invention
The detection cycle of the bio-toxicity detection method of waste water is shorter, is easy to obtain in the bio-toxicity detection field of coal chemical industrial waste water general
And it and promotes.
Description of the drawings
The bio-toxicity inspection to coal chemical industrial waste water of the present invention will hereinafter be carried out based on the embodiments and with reference to the accompanying drawings
Survey method is described in more detail, wherein：
Fig. 1 is the flow diagram of the bio-toxicity detection method for the coal chemical industrial waste water that embodiment refers to；
Fig. 2 is the scanning electron microscope (SEM) photograph for the tetrahymena that embodiment refers to；
The distribution of control group, experimental group, the first blank group and the second blank group is shown in 48 orifice plates that Fig. 3 refers to for embodiment
It is intended to, in figure, T11To T26It is the sample of experimental group, C1To C3It is the sample of control group, BT1To BT6It is the second blank group
Sample, BC1To BC3It is the sample of the first blank group；
Fig. 4 is the amount effect curve figure of tetrahymena and Daphnia magna that embodiment refers to.
Specific implementation mode
The bio-toxicity detection method of coal chemical industrial waste water of the present invention is described further below in conjunction with attached drawing.
Embodiment：The present embodiment is explained in detail with reference to Fig. 1~Fig. 4.
The bio-toxicity detection method of coal chemical industrial waste water described in the present embodiment includes：
Step 1: culture tetrahymena, obtains tetrahymena solution；
Step 2: calculating the tetrahymena density of tetrahymena solution, and judge whether tetrahymena density is less than 5 × 104cell/
Ml executes step 1 when judging result is to be, otherwise, is diluted the tetrahymena solution of predetermined volume using aseptic culture fluid
10 times, obtain tetrahymena test fluid；
Step 3: preparing control group, experimental group, the first blank group and the second blank group；
Control group be tetrahymena test fluid, experimental group be tetrahymena test fluid and water sample to be detected mixed solution, first
Blank group is aseptic culture fluid, and the second blank group is water sample to be detected；
Step 4: culture control group, experimental group, the first blank group and the second blank group；
Step 5: according to cultured control group, the absorbance of experimental group, the first blank group and the second blank group, calculate
Half-inhibition concentration of the water sample to be detected to tetrahymena；
Step 6: according to the half-inhibition concentration, the toxic equivalent of water sample to be detected is calculated.
The detailed process of the step of the present embodiment one is：
Under the conditions of 120 DEG C, sterilizing in 20 minutes is carried out to culture medium；
In an aseptic environment, culture medium is cooled down；
In an aseptic environment, tetrahymena inoculation is carried out to culture medium；
Under sterile, 25 DEG C of environment, culture medium is cultivated；
Culture medium is rocked 2~3 times daily, until tetrahymena grows to logarithmic phase；
Culture is enlarged to tetrahymena, obtains the tetrahymena solution of logarithmic phase.
The step of the present embodiment one, tetrahymena are tetrahymena thermophila；
Culture medium is PPYS culture mediums；
It is sterilized to culture medium using autoclave；
Culture medium is placed on super-clean bench cooling；
Culture medium is placed in constant incubator and is cultivated.
The step of the present embodiment two, calculates the tetrahymena density of tetrahymena solution based on blood counting chamber and microscope.
In the step of the present embodiment three, coal chemical industrial waste water is filtered using filter membrane, obtains water sample to be detected；
Control group, experimental group, the first blank group and the second blank group are held using experiment orifice plate.
In the step of the present embodiment three, control group, experimental group, the first blank group and the second blank group are placed in experimental port
Aseptic culture fluid is added dropwise in the fringe region of experiment orifice plate in the intermediate region of plate.
In the step of the present embodiment four, control group, experimental group, the training method of the first blank group and the second blank group are：
It is sterile, 25 DEG C under the conditions of, by control group, experimental group, the first blank group and the second blank group secretly set exposure 24 hours.
It is empty that the step of the present embodiment five, first uses microplate reader to measure control group, experimental group, the first blank group and second simultaneously
White absorbance of the group at 490nm, more parts of inhibition concentration data are obtained further according to inhibition concentration calculation formula；
Inhibition concentration calculation formula is：
In formula, μ is inhibition concentration, ODCIn order to control in group sample absorbance, ODBCFor the suction of sample in the first blank group
Luminosity, ODTFor the absorbance of sample in experimental group, ODBTFor the absorbance of sample in the second blank group；
By being fitted to more parts of inhibition concentration data, amount effect curve is obtained, and according to the amount effect curve, obtain half
Inhibition concentration.
Water sample to be detected is calculated according to toxic equivalent calculation formula and half-inhibition concentration in the step six roots of sensation of the present embodiment
Toxic equivalent；
Toxic equivalent calculation formula is TU=100%/EC50, and in formula, TU is toxic equivalent, and EC50 is that half inhibits dense
Degree.
The following detailed description of each step of the bio-toxicity detection method of the coal chemical industrial waste water described in the present embodiment：
Step 1：It is sterilized to PPYS culture mediums using autoclave, sterilising temp is 120 DEG C, and when sterilizing is 20 points a length of
Clock.After the completion of sterilizing, PPYS culture mediums are placed in super-clean bench and carry out natural cooling.It is aseptically, right after the completion of cooling
PPYS culture mediums carry out tetrahymena thermophila's inoculation.After the completion of inoculations, PPYS culture mediums are placed in 25 DEG C of constant incubators and are cultivated,
Often topic shaking flask 2~3 times.When tetrahymena grows to logarithmic phase, tetrahymena solution is transferred to the sterilizing PPYS of 50mL in proportion
In culture medium, transfer 3 times repeatedly or more, to be enlarged culture to tetrahymena, until it enters logarithmic phase, it is spare.
Step 2: choosing suitable tetrahymena solution, and glutaraldehyde is mixed according to 2.5% ratio.Using Burker-
The disposable tally of Turk types and optical microscope inspection are mixed with the tetrahymena solution of glutaraldehyde, and then calculate tetrahymena solution
Tetrahymena density.The scanning electron microscope image of tetrahymena is as shown in Figure 2.Two simple operation of the step of the present embodiment, through practical operation
After find：Tetrahymena density after inoculation every 24 hours can reach requirement of experiment, subsequently be observed without continuous counter, detection
It is efficient.
Step 3：Coal chemical industrial waste water is filtered using 0.45 micron membrane filter, obtains water sample to be detected.By water to be detected
Sample is added to the corresponding position of 48 orifice plates according to the volume ratio of 6 gradients.By the way that tetrahymena test fluid is added dropwise by control group
It is settled to 1ml with experimental group.Aseptic culture fluid is taken to be added dropwise to the fringe region of the first blank group and 48 orifice plates again.This step uses
Tetrahymena test fluid is added dropwise in decile pipettor.Decile pipettor can be realized, and tetrahymena test fluid etc. point is added.This step is logical
Cross setting the first blank group and the second blank group, experimental error can be effectively reduced, be significantly increased accurate testing degree and
Reduce the difference of parallel samples.In addition to this, this step in the fringe region of 48 orifice plates by way of being added aseptic culture fluid
It is evaporated come sample caused by mitigating edge effect.Found through Experimental comparison, compared to more save sample 96 orifice plates, 48 orifice plates
Using chromogenic impact caused by the high chroma of coal chemical industrial waste water can be effectively prevented.Control group, experimental group, first in 48 orifice plates
The distribution schematic diagram of blank group and the second blank group is as shown in Figure 3.
Step 4：It is compared through preliminary experiment, preferable in coal chemical industrial waste water water quality, the detection result of exposure for 24 hours is bright
It is aobvious.Extension rate detection need to be increased when the bio-toxicity of coal chemical industrial waste water or higher coloration.It may contain in coal chemical industrial waste water
There are phenols, phenols that light reaction easily occurs and water sample to be detected is made to change colour, and then detection is had an impact.Therefore, this step will be controlled
Processed group, experimental group, the first blank group and the second blank group secretly set culture.
Step 5：Origin fittings are carried out to more parts of inhibition concentration data, obtain amount effect curve.
Step 6：Toxic equivalent calculation formula adopted in this step is TU=100%/EC50, and in formula, TU works as toxicity
Amount, EC50 is half-inhibition concentration.If water sample to be detected is not diluted and generates half effect, is i.e. TU=1, but according to
This programme usually cannot achieve former water determination, and actual waste water testing result is all higher than 1, minimum dilute if effluent quality is preferable
It releases multiple and cannot get half effect, then 0.4TU (amount effect curve can acquire) is corresponded to according to EC20 effects, to calculate actual water sample
Toxic unit.
The bio-toxicity detection method of coal chemical industrial waste water described in the present embodiment has simple operation, of low cost, saving
Many advantages, such as efficiently and reproducibility is high can be that the toxicity evaluation of coal chemical industrial waste water establishes the index of system, have wide
Application prospect.
In order to further verify sensibility of the tetrahymena to coal chemical industrial waste water, the present embodiment has also carried out subsequent experimental, with
Compare toxicity grading result of the coal chemical industrial waste water to tetrahymena and international tested species Daphnia magna.
According to《Water quality substance is to Daphnia magna determination of acute toxicity method》(GB/T 13266-91), will hatching 6~for 24 hours, body
Fail to grow up in the young Magna of 1mm be added more concentration gradients coal chemical industrial waste water (using Daphnia magna test diluent preparing) secretly set training
It is counted after supporting 48h, and calculates inhibition concentration according to the following formula：
In formula, inhibition number is slight vibration culture solution, in 15s without the Daphnia magna quantity obviously moved.
Table 1 is the testing result of tetrahymena microplate reader output：
The testing result of tetrahymena microplate reader output
There is very strong relevance according between 1 parallel samples of table.
Fig. 4 is the amount effect curve figure of tetrahymena and Daphnia magna.The amount effect curve degree of fitting of two kinds of biological subjects is above
95%, illustrate the reasonability of tetrahymena method, and EC50 passes through calculating TU values, TU Daphnia magnas=2.726, TU tetra- close to overlapping
Film worm=2.240, toxicity grading are acute toxicity, illustrate that two kinds of biological sensibility are closer to.The present embodiment is used
Various raw materials and items of equipment be conventional commercial product, can by market buy directly obtain.Wherein, for trying four films
Worm is by the aquatic tetrahymena thermophila SB210 provided in Chinese Academy of Sciences Wuhan, and the Daphnia magna of contrast test is from Chinese Academy of Sciences's Shenyang ecology
Research institute tests the HandyStep etc. that decile pipettor used is Germany Brand manufactures and pipettor, microplate reader is divided to be the U.S.
The Elx800 type microplate reader of Biotek manufactures, cell counting board are the Burker-Turk type cell counts of Japan Watson manufactures
Plate.
It is the operation in actual experiment below：
Use self-control PPYS culture mediums (peptone that mass concentration is 1%, 0.1% yeast extraction powder, 0.2% Portugal
Grape sugar), prepare culture medium in proportion, according to actual test as a result, this programme that 50~100mg Luo Hong are added in 1L culture mediums is mould
Element can effectively inhibit later stage test sample microbiological contamination and be had no significant effect to tetrahymena growth, culture medium is then put into autoclave
Sterilize 20min at 120 DEG C.It is put into super-clean bench cooling after taking-up, is inoculated under aseptic condition.Inoculation is completed, and perseverance is placed in
It is cultivated in warm incubator.Condition of culture is 25 DEG C ± 0.5 DEG C and is protected from light culture, and daily oscillation 3 times will be grown for 24 hours to logarithmic phase
Worm liquid is transferred in 2% ratio in 50mL sterilising mediums, and transfer 3 times is enlarged culture repeatedly to tetrahymena, grows
It is spare to logarithmic phase.
The selection of test condition
Test period presses 6h, 12h in preliminary experiment and is compared with experimental result for 24 hours, it is found that Toxicity of Water Samples is higher and dilutes again
6h, 12h inhibit more apparent when number is relatively low, but the growth of control group tetrahymena is often not enough to distinguish with experimental group absorbance, for 24 hours
Absorbance caused by control group tetrahymena concentration is distinguished with experimental group absorbance enough after exposure.It utilizes etc. and to divide pipettor, it can be real
Existing microorganism etc. point is added, while the setting of the first blank group and the second blank group can effectively reduce systematic error, significantly
It improves accurate testing degree and reduces the difference of parallel samples.The culture solution that last outer edge is added can effectively mitigate edge effect and draw
The sample evaporation risen.In addition, Experimental comparison has found, compared to 96 orifice plates for more saving sample, the utilization of 48 orifice plates can effectively be prevented
Only chromogenic impact caused by coal chemical industrial waste water high chroma.
The drafting of amount effect curve
Due to testing at least two groups of Duplicate Samples every time, the average value that can use test draws amount effect curve, real to improve
Accuracy is tested, can also simultaneously be tested using two 48 orifice plates, screening wherein more rational 3 groups of data are drawn according to standard error
Amount effect curve.Toxic unit and the classification of water sample are obtained according to EC20, EC50 value combination TU calculation formula of output.
Although describing the present invention herein with reference to specific embodiment, it should be understood that, these realities
It is the example of principles and applications to apply example only.It should therefore be understood that can be permitted exemplary embodiment
More modifications, and can be designed that other arrangements, spirit without departing from the present invention as defined in the appended claims and
Range.It should be understood that can be by combining different appurtenances different from mode described in original claim
It is required that and feature described herein.It will also be appreciated that the feature in conjunction with described in separate embodiments can be used at it
In his embodiment.
Claims (10)
1. a kind of bio-toxicity detection method of coal chemical industrial waste water, which is characterized in that the bio-toxicity detection method uses four
Film worm is as toxotest species.
2. the bio-toxicity detection method of coal chemical industrial waste water as described in claim 1, which is characterized in that the bio-toxicity inspection
Survey method includes：
Step 1: culture tetrahymena, obtains tetrahymena solution；
Step 2: calculating the tetrahymena density of tetrahymena solution, and judge whether tetrahymena density is less than 5 × 104Cell/ml, when
Judging result is when being, to execute step 1, otherwise, the tetrahymena solution of predetermined volume is diluted 10 times using aseptic culture fluid,
Obtain tetrahymena test fluid；
Step 3: preparing control group, experimental group, the first blank group and the second blank group；
Control group is tetrahymena test fluid, and experimental group is the mixed solution of tetrahymena test fluid and water sample to be detected, the first blank
Group is aseptic culture fluid, and the second blank group is water sample to be detected；
Step 4: culture control group, experimental group, the first blank group and the second blank group；
Step 5: according to cultured control group, the absorbance of experimental group, the first blank group and the second blank group, calculate to be checked
Survey half-inhibition concentration of the water sample to tetrahymena；
Step 6: according to the half-inhibition concentration, the toxic equivalent of water sample to be detected is calculated.
3. the bio-toxicity detection method of coal chemical industrial waste water as claimed in claim 2, which is characterized in that the specific stream of step 1
Cheng Wei：
Under the conditions of 120 DEG C, sterilizing in 20 minutes is carried out to culture medium；
In an aseptic environment, culture medium is cooled down；
In an aseptic environment, tetrahymena inoculation is carried out to culture medium；
Under sterile, 25 DEG C of environment, culture medium is cultivated；
Culture medium is rocked 2~3 times daily, until tetrahymena grows to logarithmic phase；
Culture is enlarged to tetrahymena, obtains the tetrahymena solution of logarithmic phase.
4. the bio-toxicity detection method of coal chemical industrial waste water as claimed in claim 3, which is characterized in that in step 1,
Tetrahymena is tetrahymena thermophila；
Culture medium is PPYS culture mediums；
It is sterilized to culture medium using autoclave；
Culture medium is placed on super-clean bench cooling；
Culture medium is placed in constant incubator and is cultivated.
5. the bio-toxicity detection method of coal chemical industrial waste water as claimed in claim 2, which is characterized in that step 2 is based on blood cell
Tally and microscope calculate the tetrahymena density of tetrahymena solution.
6. the bio-toxicity detection method of coal chemical industrial waste water as claimed in claim 2, which is characterized in that in step 3,
Coal chemical industrial waste water is filtered using filter membrane, obtains water sample to be detected；
Control group, experimental group, the first blank group and the second blank group are held using experiment orifice plate.
7. the bio-toxicity detection method of coal chemical industrial waste water as claimed in claim 6, which is characterized in that, will in step 3
Control group, experimental group, the first blank group and the second blank group are placed in the intermediate region of experiment orifice plate, at the edge of experiment orifice plate
Aseptic culture fluid is added dropwise in region.
8. the bio-toxicity detection method of coal chemical industrial waste water as claimed in claim 2, which is characterized in that in step 4, control
Processed group, experimental group, the training method of the first blank group and the second blank group be：It is sterile, 25 DEG C under the conditions of, by control group, reality
It tests group, the first blank group and the second blank group and secretly sets exposure 24 hours.
9. the bio-toxicity detection method of coal chemical industrial waste water as claimed in claim 2, which is characterized in that step 5 first uses enzyme
Mark instrument measures the absorbance of control group, experimental group, the first blank group and the second blank group at 490nm simultaneously, further according to inhibition
Concentration calculation formula obtains more parts of inhibition concentration data；
Inhibition concentration calculation formula is：
In formula, μ is inhibition concentration, ODCIn order to control in group sample absorbance, ODBCFor the absorbance of sample in the first blank group,
ODTFor the absorbance of sample in experimental group, ODBTFor the absorbance of sample in the second blank group；
By being fitted to more parts of inhibition concentration data, amount effect curve is obtained, and according to the amount effect curve, obtain half and inhibit
Concentration.
10. the bio-toxicity detection method of coal chemical industrial waste water as claimed in claim 2, which is characterized in that step 6 is according to poison
Property equivalent calculation formula and half-inhibition concentration, are calculated the toxic equivalent of water sample to be detected；
Toxic equivalent calculation formula is TU=100%/EC50, and in formula, TU is toxic equivalent, and EC50 is half-inhibition concentration.
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| CN111996229A (en) * | 2020-07-29 | 2020-11-27 | 广东省微生物研究所（广东省微生物分析检测中心） | Method for monitoring toxicity of ionic liquid by using tetrahymena |
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