CN105693308A - Fast detonating method for low-temperature composite - Google Patents
Fast detonating method for low-temperature composite Download PDFInfo
- Publication number
- CN105693308A CN105693308A CN201610269477.9A CN201610269477A CN105693308A CN 105693308 A CN105693308 A CN 105693308A CN 201610269477 A CN201610269477 A CN 201610269477A CN 105693308 A CN105693308 A CN 105693308A
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- CN
- China
- Prior art keywords
- low
- temperature
- windrow
- quickly
- compost
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a fast detonating method for low-temperature composite.The method solves the problems that time consumed in the detonating stage in a low-temperature environment is long, and manual heating cost is high.According to the method, low-temperature cryogenic amylase is mixed in windrow, and fast detonating of the low-temperature composite can be achieved.The method is simple, operation is easy, cost is low, the effect is good, the low-temperature cryogenic amylase directly acts on the windrow, and the detonating time of the composite is shortened within 24 h.
Description
Technical field
The present invention relates to a kind of method that compost quickly detonates。
Background technology
Fertilising can not only be increase soil fertility, and is the important measures improving crop yield per unit area。Chemical fertilizer be agricultural production the most basic and also be most important material put into, simple and easy to use。But along with the use of chemical fertilizer, it increases heavy metal in soil and toxic element, reduces microbial activity, material is difficult to convert and degrade, causing soil nutrient imbalance, nitrates accumulation, soil the problem such as is finished and is day by day highlighted。
Compost belongs to organic fertilizer, and contained nutrient substance is abundanter, and and fertilizer efficiency length stablize, may advantageously facilitate the formation of the solid kernel structure of soil simultaneously, the ability of soil water-retaining, insulation, ventilative, fertilizer conservation can be increased；And, compost is one of main method of reclaiming organic waste process。The use of compost can reduce the consumption that makes of chemical fertilizer, reduces the harmful effect to environment and soil。
The uphill process of compost temperature is the result of compost initial temperature, bacteria metabolism exothermal process and heap body insulation effect comprehensive function。Heap body generally to experience temperature-rise period, high temperature time-continuing process and temperature-fall period, but when the initial temperature of compost material is very low, heap body will also be subjected to one from low temperature to the stage of 15 DEG C, this stage is significant for the amount reproduction of microorganism and the startup of composting process, is referred to as the stage of detonating。Winter climate severe cold that high latitude area (is referred mainly to 40 ° of northern areas of north latitude, including Northern-Northeast China, North of North China and the north, northwest etc.), ice period, the lowest temperature was many below-30 DEG C generally up to 3～6 months。Carrying out compost under low temperature environment, the initial temperature of raw material is lower than 15 DEG C, and even up to 0 DEG C, composting process is slack-off even to be stopped, although and the temperature of raw material can be made to rise to 15 DEG C by the method for Heat artificially, but cost can be increased in large quantities。
Summary of the invention
The invention aims to solve to detonate under low temperature environment stage length consuming time, Heat artificially high in cost of production problem, and the method that a kind of low temperature compost provided quickly detonates。
The method that low temperature compost of the present invention quickly detonates: low-temperature amylase is mixed into windrow and becomes thoroughly decomposed, can realize low temperature compost and quickly detonate。
Present invention, avoiding the function added microbial inoculum or composite bacteria agent capable and need breeding to cause to postpone, and the competition that inoculating microbe and indigenous microorganism are in digest process, directly utilize low-temperature amylase and decompose the simple organics such as the sugar in windrow, starch, make heap temperature raise rapidly, be rapidly completed the stage of detonating。Simply, easily operate, cost is low, effective, and low-temperature amylase directly acts on windrow for the inventive method, within the compost burst time shortens to 24h。
Low-temperature amylase liquid adds windrow according to the ratio of 0.5%～2%, and the enzyme work of low-temperature amylase liquid is 10～35U/mL, and the windrow of 4 DEG C can be made quickly to bring up to 20 DEG C when 4 DEG C in 24h。
Accompanying drawing explanation
Fig. 1 is embodiment 1 compost temperature change curve, and wherein " ■ " curve is ambient temperature curve residing for windrow, and " ◆ " curve is windrow temperature curve。
Detailed description of the invention
Technical solution of the present invention is not limited to act detailed description of the invention set forth below, also includes the combination in any between each detailed description of the invention。
Detailed description of the invention one: the method that present embodiment low temperature compost quickly detonates: low-temperature amylase is mixed into windrow and becomes thoroughly decomposed, can realize low temperature compost and quickly detonate。
Detailed description of the invention two: the present embodiment difference from detailed description of the invention one is: windrow includes the changing food waste of mechanical expression dehydration。Other step and parameter and embodiment one are identical。
Detailed description of the invention three: the present embodiment difference from detailed description of the invention one or two is: windrow temperature >=0 DEG C。Other step and parameter and embodiment one or two are identical。
Detailed description of the invention four: the present embodiment difference from one of detailed description of the invention one to three is: compost environment temperature >=-10 DEG C。Other step and one of parameter and embodiment one to three are identical。
Detailed description of the invention five: the present embodiment difference from one of detailed description of the invention one to four is: the addition of low-temperature amylase liquid is the 0.5%～2% of windrow quality, and the enzyme work of low-temperature amylase liquid is 10～35U/mL。Other step and one of parameter and embodiment one to four are identical。
Detailed description of the invention six: the present embodiment difference from one of detailed description of the invention one to five is: windrow moisture content is 50%～70%。Other step and one of parameter and embodiment one to five are identical。
Embodiment 1
Low-temperature amylase product is made the enzyme low-temperature amylase liquid for 10U/mL alive, then presses the 2% of windrow quality and add, and mix with windrow；Wherein, windrow includes the weight ratio of primary changing food waste and rice husk, changing food waste and rice husk is 32；Adjusting windrow moisture content about 60%, windrow temperature is 4 DEG C。
The present embodiment carries out compost, ambient temperature and windrow temperature as shown in Figure 1 in the environment of 4 DEG C。In 24h, windrow temperature quickly brings up to 20 DEG C。
Embodiment 2
The self-control thick liquid of low-temperature amylase:
Weigh the kitchen waste compost sample 2g preserving 2 years under-20 DEG C of conditions, by 20mL aquesterilisa oscillation cleaning, standing, to be precipitated completely after take supernatant 1mL and add in enrichment medium of 50mL, in 4 DEG C, 200r/min shaken cultivation 3d, take 1mL culture fluid again and add in No. bis-enrichment mediums of 50mL, in 4 DEG C, 200r/min continue shaken cultivation 3d, obtain the culture fluid of composite microbial system；The culture fluid being stained with a small amount of composite microbial system with inoculating loop draws a vertical line in the middle part of starch plating medium, 4 DEG C of quiescent culture 3d, take (the configuration of 0.1mol/L iodine liquid: weigh 13g iodine and 35g potassium iodide is dissolved in 1000mL water of 0.02mol/L iodine liquid, take 0.1mol/L iodine liquid and dilute 5 times) or Lugol's iodine solution dropping periphery of bacterial colonies in starch flat board, composite microbial system bacterium colony forms transparent circle on starch culture-medium, it was shown that have the bacterial strain of secretion low-temperature amylase in composite microbial system；
The composite microbial system culture fluid having secretion low-temperature amylase bacterial strain is accessed in fermentation medium by 4% inoculum concentration, in 4 DEG C, 200r/min shaken cultivation 2d, again by fermentation culture 4 DEG C, 200r/min, centrifugal 10min, taking the supernatant crude enzyme liquid as low-temperature amylase, the enzyme of crude enzyme liquid is lived as 33.6U/mL。
It is Bacillussubtilis, Bacillustequilensis and Brevibacillusborstelensis that separated purification cultivates main group in the composite microbial system confirming there is secretion low-temperature amylase bacterial strain。
Culture medium prescription:
Enrichment medium: soluble starch 2g, peptone 1g, Carnis Bovis seu Bubali cream 0.5g, NH4NO31g, KH2PO41g, FeSO4·7H2O0.01g, MgSO4·7H2O0.5g, KCl0.5g, deionized water 1000mL, natural pH, 121 DEG C of sterilizing 20min。
Secondary enrichment medium: soluble starch 2g, NH4NO31g, KH2PO41g, FeSO4·7H2O0.01g, MgSO4·7H2O0.5g, KCl0.5g, deionized water 1000mL, natural pH, 121 DEG C of sterilizing 20min。
Starch plating medium: soluble starch 2g, peptone 10g, yeast extract 5g, K2HPO43g, FeSO4·7H2O0.001g, MgSO4·7H2O0.05g, agar 20g, deionized water 1000mL, pH7.0～7.2,121 DEG C of sterilizing 20min。
Fermentation medium: soluble starch 5g, peptone 5g, yeast extract 5g, NaCl1g, FeSO4·7H2O0.001g, MgSO4·7H2O0.05g, deionized water 1000mL, pH7.0,121 DEG C of sterilizing 20min。
The mass percent of the crude enzyme liquid 1% of the low-temperature amylase prepared by the present embodiment adds windrow, and mixes with windrow；Wherein, windrow includes changing food waste and the rice husk of mechanical expression dehydration, and the weight ratio of changing food waste and rice husk is 5:2；Windrow moisture content is 60%, and windrow temperature is 4 DEG C。
The present embodiment carries out compost in the environment of 4 DEG C, and compost 24h windrow temperature quickly brings up to more than 20 DEG C。
Claims (7)
1. the method that a low temperature compost quickly detonates, it is characterised in that low-temperature amylase is mixed into windrow and becomes thoroughly decomposed by the method, can realize low temperature compost and quickly detonate。
2. the method that a kind of low temperature compost according to claim 1 quickly detonates, it is characterised in that windrow temperature >=0 DEG C。
3. the method that a kind of low temperature compost according to claim 1 quickly detonates, it is characterised in that compost environment temperature >=-10 DEG C。
4. the method that a kind of low temperature compost according to claim 1 quickly detonates, it is characterised in that the addition of low-temperature amylase liquid is the 0.5%～2% of windrow quality, the enzyme work of low-temperature amylase liquid is 10～35U/mL。
5. the method that a kind of low temperature compost according to claim 1 quickly detonates, it is characterised in that windrow moisture content is 50%～70%。
6. the method that a kind of low temperature compost according to claim 5 quickly detonates, it is characterised in that windrow includes the changing food waste of mechanical expression dehydration。
7. the method that a kind of low temperature compost according to claim 6 quickly detonates, it is characterised in that windrow includes changing food waste and the rice husk of mechanical expression dehydration, the weight ratio of changing food waste and rice husk is 5:2。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610269477.9A CN105693308A (en) | 2016-04-27 | 2016-04-27 | Fast detonating method for low-temperature composite |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610269477.9A CN105693308A (en) | 2016-04-27 | 2016-04-27 | Fast detonating method for low-temperature composite |
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| CN105693308A true CN105693308A (en) | 2016-06-22 |
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| CN201610269477.9A Pending CN105693308A (en) | 2016-04-27 | 2016-04-27 | Fast detonating method for low-temperature composite |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101786911A (en) * | 2010-02-04 | 2010-07-28 | 北京微邦生物工程有限公司 | Microorganism and enzyme powder composite biological agent for microbial organic fertilizer |
| CN103361282A (en) * | 2012-03-28 | 2013-10-23 | 北京沃土天地生物科技有限公司 | Low temperature resistant cellulose degradation bacteria and application thereof |
| CN104611267A (en) * | 2015-02-03 | 2015-05-13 | 东北农业大学 | Household garbage low-temperature high-efficiency degrading functional complex microbial inoculant and preparation method and application thereof |
-
2016
- 2016-04-27 CN CN201610269477.9A patent/CN105693308A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101786911A (en) * | 2010-02-04 | 2010-07-28 | 北京微邦生物工程有限公司 | Microorganism and enzyme powder composite biological agent for microbial organic fertilizer |
| CN103361282A (en) * | 2012-03-28 | 2013-10-23 | 北京沃土天地生物科技有限公司 | Low temperature resistant cellulose degradation bacteria and application thereof |
| CN104611267A (en) * | 2015-02-03 | 2015-05-13 | 东北农业大学 | Household garbage low-temperature high-efficiency degrading functional complex microbial inoculant and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 韩玮，等: "添加外源纤维素酶对水稻秸秆模拟堆肥过程的影响", 《环境工程学报》 * |
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Application publication date: 20160622 |
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