CN105688210A - Yolk biological protein skin repair dressing and preparation method thereof - Google Patents
Yolk biological protein skin repair dressing and preparation method thereof Download PDFInfo
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
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Abstract
The invention discloses a yolk biological protein liquid and a yolk biological protein skin repair dressing prepared from the yolk biological protein liquid. The yolk biological protein skin repair dressing is prepared from main components as follows: 1%-5% of multiple yolk protein, 0.1%-0.3% of an epidermal growth factor and the balance of a hyaluronic acid solution, wherein the multiple yolk protein is an egg yolk antibody obtained by performing injection immunity on healthy hens, taking eggs laid by the hens and performing separation and purification with one or more pathogens capable of causing skin infection used as immunogen. The multiple yolk protein can inactivate various pathogens causing skin infection in a targeted manner, promote operation wound recovery and effectively prevent bacterial infection and causes no irritation to human bodies, and the added epidermal growth factor can repair wounds, promote epidermal wound surfaces to restore quickly, moisten skin and eliminate winkles and scars, has better biocompatibility with the multiple yolk protein, causes no dependence and drug resistance, has no allergy and is safe and efficient.
Description
Technical field
The present invention relates to the protection of skin and nursing technique field, be specifically related to a kind of yolk bioprotein skin repair dressing and preparation method thereof。
Background technology
Medical science shaping and beauty is following development trend, for instance face laser, photon delicate skin, crystallite grinding, fruit acid skin-active art etc., wound surface is nursed by postoperative needs of these medical and beauty treatments。
And traditional product composition is mainly based on Chinese medicine, chemical medicine, antibiotic at present。Conventional medicament can produce side effect in various degree when effect, and is difficult to selectively killing pathogen。For various operation wounds, what some were relatively advanced can promote that the medical dressing recovering energy is perfect not enough, can not effectively prevent antibacterial from infecting, and rapidly promote epidermis wound surface fast restore。
Therefore; the purpose of the present invention is aiming at above deficiency; develop a kind of skin repair dressing based on yolk bioprotein; it is applicable not only to protection and the nursing of the post-surgical trauma skins such as face laser, photon delicate skin, crystallite grinding, fruit acid skin-active art, applies also for protection and the nursing of the barrier damaged skins such as dermatitis and eczema, sensitive skin, steroid-dependent dermatitis。
Summary of the invention
First technical problem that this invention address that is to provide and a kind of can inactivate the multiple pathogen causing skin infection and can repair the yolk bioprotein liquid of impaired skin by targeting。
Second technical problem that this invention address that is to provide a kind of yolk bioprotein skin repair dressing comprising described yolk bioprotein liquid and preparation method thereof。
For solving above-mentioned first technical problem, the technical solution adopted in the present invention is:
A kind of yolk bioprotein liquid, by mass percentage, main component is as follows: multi-joint livetin 1-5%, epidermal growth factor 0.1-0.3%, surplus are hyaluronic acid solution, described multi-joint livetin is the pathogen of skin infection can be caused as immunogen using one or more, hen is carried out injecting immune, takes the egg of hen production and separate the yolk antibody that purification obtains。
Further, in such scheme, described pathogen includes but not limited to propionibacterium acnes, leprosy bacillus, gonococcus, vermiform mite, acaricide。
Further, in such scheme, the preparation method of described multi-joint livetin is:
(1) propionibacterium acnes leprosy bacillus, gonococcus, vermiform mite, acaricide are cultivated respectively by culture medium；The thalline obtained uses formalin-inactivated respectively, and oil emulsion inactivated vaccine is made in emulsifying；
(2) inactivated vaccine that hen obtains by above-mentioned steps (1) respectively being carried out immunity, the egg the separation that take hen production obtain yolk liquid；
(3) egg yolk liquid that step (2) obtains is mixed by the volume ratio of 1:1-5 with sterile purified water, 350-500rpm vibrates 15-30min, the volume ratio pressing 1:2 again adds sterile purified water, 600rpm vibrates 5-10min, regulate pH to 5.1,4 DEG C stand 10 hours, then centrifugal 10 minutes of 6000rpm, collect supernatant；
(4) adding in the supernatant that step (3) obtains by equal-volume saturated ammonium sulfate aqueous solution, 4 DEG C stand 12 hours, and centrifugal 20 minutes of 8000rpm takes precipitation；Dissolve obtained precipitation by the mass ratio distilled water of 1:6, obtain lysate, be subsequently adding the caprylic acid relative to gained lysate 1.3 ‰, mixing；
(5) by the volume ratio of 1:2, saturated ammonium sulfate aqueous solution and step (4) are obtained lysate to mix, 4 DEG C stand 12 hours, centrifugal 20 minutes of 8000rpm, take precipitation, dissolve with PBS aseptic, pH7.2,0.01M by the mass ratio of 1:4, the solution 100Ku ultrafilter membrane bag obtained carries out desalination concentration, obtains concentrated solution；
(6) adding the formaldehyde of 0.05-0.1% in the concentrated solution that step (5) obtains, then room temperature stands 3-5 hour in sealed states, then with filtering with microporous membrane, obtains filtrate, is yolk antibody。
Preferably, described hen for without artificial immunity, open product and normal healthy hens of laying eggs。
Further, in such scheme, the concentration of described hyaluronic acid solution is 6-8g/L, and this hyaluronic acid solution is gel, and swelling ratio is high, and gel elastomer intensity is big, has the effect promoting skin epidermal cells propagation preferably。
For solving above-mentioned second technical problem, the technical solution adopted in the present invention is:
A kind of yolk bioprotein skin repair dressing comprising described yolk bioprotein liquid, is made up of according to certain proportioning described yolk bioprotein liquid and sodium alginate, polyvinyl alcohol, chitosan, sorbitol, glycerol, compound vitamin, fibroin albumen。
Further, its preparation method is: add in described yolk bioprotein liquid relative to the sodium alginate of this yolk bioprotein liquid weight 0.3-1.6%, the polyvinyl alcohol of 2-5%, the chitosan of 1-5%, the sorbitol of 0.1-0.5%, the glycerol of 1-1.5%, 0.2-1% compound vitamin, 0.3-2% fibroin albumen, stir, namely obtain described yolk bioprotein skin repair dressing。Described compound vitamin by the 1:1.2:2 mixture formed, is not only had the function of antioxidation, scavenging free radicals by vitamin A, vitamin C, vitamin E, moreover it is possible to promote epidermal growth。
Further, in such scheme, described whipping process keeps temperature of charge be 35-50 DEG C, improve the degrees of fusion of material。
The invention has the beneficial effects as follows: the multi-joint livetin of the present invention can inactivate the multiple pathogen causing skin infection by targeting, can promote that operation wound recovers, and can effectively prevent antibacterial from infecting, human body is non-stimulated, affiliated epidermal growth factor can not only repair wound, promote epidermis wound surface fast restore, additionally it is possible to skin care, smooth away wrinkles, scar, better with the biocompatibility of multi-joint livetin, do not produce dependency and drug resistance, without anaphylaxis, safe and efficient。Suitable in protection and the nursing of the barrier damaged skins such as dermatitis and eczema, sensitive skin, steroid-dependent dermatitis, it is simultaneously suitable for protection and the nursing of the post-surgical trauma skins such as face laser, photon delicate skin, crystallite grinding, fruit acid skin-active art。
Detailed description of the invention
Embodiment 1:
A kind of yolk bioprotein liquid, by mass percentage, main component is as follows: multi-joint livetin 1%, epidermal growth factor 0.1%, surplus are hyaluronic acid solution, described multi-joint livetin is the pathogen of skin infection can be caused as immunogen using one or more, hen is carried out injecting immune, takes the egg of hen production and separate the yolk antibody that purification obtains。
Wherein, the concentration of described hyaluronic acid solution is 6g/L, and this hyaluronic acid solution is gel, and swelling ratio is high, and gel elastomer intensity is big, has the effect promoting skin epidermal cells propagation preferably。Described pathogen includes but not limited to propionibacterium acnes, leprosy bacillus, gonococcus, vermiform mite, acaricide。
The preparation method of described multi-joint livetin is:
(1) propionibacterium acnes leprosy bacillus, gonococcus, vermiform mite, acaricide are cultivated respectively by culture medium；The thalline obtained uses formalin-inactivated respectively, and oil emulsion inactivated vaccine is made in emulsifying；
(2) inactivated vaccine that hen obtains by above-mentioned steps (1) respectively is carried out immunity, it is preferable that described hen is without artificial immunity, opens product and normal healthy hens of laying eggs, and the egg the separation that take hen production obtain yolk liquid；
(3) egg yolk liquid that step (2) obtains is mixed by the volume ratio of 1:1-5 with sterile purified water, 350rpm vibrates 15min, the volume ratio pressing 1:2 again adds sterile purified water, 600rpm vibrates 5min, regulate pH to 5.1,4 DEG C stand 10 hours, then centrifugal 10 minutes of 6000rpm, collect supernatant；
(4) adding in the supernatant that step (3) obtains by equal-volume saturated ammonium sulfate aqueous solution, 4 DEG C stand 12 hours, and centrifugal 20 minutes of 8000rpm takes precipitation；Dissolve obtained precipitation by the mass ratio distilled water of 1:6, obtain lysate, be subsequently adding the caprylic acid relative to gained lysate 1.3 ‰, mixing；
(5) by the volume ratio of 1:2, saturated ammonium sulfate aqueous solution and step (4) are obtained lysate to mix, 4 DEG C stand 12 hours, centrifugal 20 minutes of 8000rpm, take precipitation, dissolve with PBS aseptic, pH7.2,0.01M by the mass ratio of 1:4, the solution 100Ku ultrafilter membrane bag obtained carries out desalination concentration, obtains concentrated solution；
(6) adding the formaldehyde of 0.05% in the concentrated solution that step (5) obtains, then room temperature stands 3 hours in sealed states, then with filtering with microporous membrane, obtains filtrate, is yolk antibody。
Comprise the yolk bioprotein skin repair dressing of described yolk bioprotein liquid, be made up of according to certain proportioning described yolk bioprotein liquid and sodium alginate, polyvinyl alcohol, chitosan, sorbitol, glycerol, compound vitamin, fibroin albumen。Described compound vitamin by the 1:1.2:2 mixture formed, is not only had the function of antioxidation, scavenging free radicals by vitamin A, vitamin C, vitamin E, moreover it is possible to promote epidermal growth。
Its preparation method is: add in described yolk bioprotein liquid relative to the sodium alginate of this yolk bioprotein liquid weight 0.3%, 2% polyvinyl alcohol, 1% chitosan, 0.1% sorbitol, 1% glycerol, 0.2% compound vitamin, 0.3% fibroin albumen, temperature of charge 35 DEG C is kept to be stirred, improve the degrees of fusion of material, stir, namely obtain described yolk bioprotein skin repair dressing。
Embodiment 2:
A kind of yolk bioprotein liquid, by mass percentage, main component is as follows: multi-joint livetin 3%, epidermal growth factor 0.2%, surplus are hyaluronic acid solution, described multi-joint livetin is the pathogen of skin infection can be caused as immunogen using one or more, hen is carried out injecting immune, takes the egg of hen production and separate the yolk antibody that purification obtains。
Wherein, the concentration of described hyaluronic acid solution is 7g/L, and this hyaluronic acid solution is gel, and swelling ratio is high, and gel elastomer intensity is big, has the effect promoting skin epidermal cells propagation preferably。Described pathogen includes but not limited to propionibacterium acnes, leprosy bacillus, gonococcus, vermiform mite, acaricide。
The preparation method of described multi-joint livetin is:
(1) propionibacterium acnes leprosy bacillus, gonococcus, vermiform mite, acaricide are cultivated respectively by culture medium；The thalline obtained uses formalin-inactivated respectively, and oil emulsion inactivated vaccine is made in emulsifying；
(2) inactivated vaccine that hen obtains by above-mentioned steps (1) respectively is carried out immunity, it is preferable that described hen is without artificial immunity, opens product and normal healthy hens of laying eggs, and the egg the separation that take hen production obtain yolk liquid；
(3) egg yolk liquid that step (2) obtains is mixed by the volume ratio of 1:3 with sterile purified water, 425rpm vibrates 22.5min, the volume ratio pressing 1:2 again adds sterile purified water, 600rpm vibrates 5-10min, regulate pH to 5.1,4 DEG C stand 10 hours, then centrifugal 10 minutes of 6000rpm, collect supernatant；
(4) adding in the supernatant that step (3) obtains by equal-volume saturated ammonium sulfate aqueous solution, 4 DEG C stand 12 hours, and centrifugal 20 minutes of 8000rpm takes precipitation；Dissolve obtained precipitation by the mass ratio distilled water of 1:6, obtain lysate, be subsequently adding the caprylic acid relative to gained lysate 1.3 ‰, mixing；
(5) by the volume ratio of 1:2, saturated ammonium sulfate aqueous solution and step (4) are obtained lysate to mix, 4 DEG C stand 12 hours, centrifugal 20 minutes of 8000rpm, take precipitation, dissolve with PBS aseptic, pH7.2,0.01M by the mass ratio of 1:4, the solution 100Ku ultrafilter membrane bag obtained carries out desalination concentration, obtains concentrated solution；
(6) adding the formaldehyde of 0.075% in the concentrated solution that step (5) obtains, then room temperature stands 4 hours in sealed states, then with filtering with microporous membrane, obtains filtrate, is yolk antibody。
Comprise the yolk bioprotein skin repair dressing of described yolk bioprotein liquid, be made up of according to certain proportioning described yolk bioprotein liquid and sodium alginate, polyvinyl alcohol, chitosan, sorbitol, glycerol, compound vitamin, fibroin albumen。Described compound vitamin by the 1:1.2:2 mixture formed, is not only had the function of antioxidation, scavenging free radicals by vitamin A, vitamin C, vitamin E, moreover it is possible to promote epidermal growth。
Its preparation method is: add in described yolk bioprotein liquid relative to the sodium alginate of this yolk bioprotein liquid weight 0.95%, 3.5% polyvinyl alcohol, 3% chitosan, 0.3% sorbitol, 1.25% glycerol, 0.6% compound vitamin, 1.15% fibroin albumen, temperature of charge 42.5 DEG C is kept to be stirred, improve the degrees of fusion of material, stir, namely obtain described yolk bioprotein skin repair dressing。
Embodiment 3:
A kind of yolk bioprotein liquid, by mass percentage, main component is as follows: multi-joint livetin 5%, epidermal growth factor 0.3%, surplus are hyaluronic acid solution, described multi-joint livetin is the pathogen of skin infection can be caused as immunogen using one or more, hen is carried out injecting immune, takes the egg of hen production and separate the yolk antibody that purification obtains。
Wherein, the concentration of described hyaluronic acid solution is 8g/L, and this hyaluronic acid solution is gel, and swelling ratio is high, and gel elastomer intensity is big, has the effect promoting skin epidermal cells propagation preferably。Described pathogen includes but not limited to propionibacterium acnes, leprosy bacillus, gonococcus, vermiform mite, acaricide。
The preparation method of described multi-joint livetin is:
(1) propionibacterium acnes leprosy bacillus, gonococcus, vermiform mite, acaricide are cultivated respectively by culture medium；The thalline obtained uses formalin-inactivated respectively, and oil emulsion inactivated vaccine is made in emulsifying；
(2) inactivated vaccine that hen obtains by above-mentioned steps (1) respectively is carried out immunity, it is preferable that described hen is without artificial immunity, opens product and normal healthy hens of laying eggs, and the egg the separation that take hen production obtain yolk liquid；
(3) egg yolk liquid that step (2) obtains is mixed by the volume ratio of 1:5 with sterile purified water, 500rpm vibrates 30min, the volume ratio pressing 1:2 again adds sterile purified water, 600rpm vibrates 5-10min, regulate pH to 5.1,4 DEG C stand 10 hours, then centrifugal 10 minutes of 6000rpm, collect supernatant；
(4) adding in the supernatant that step (3) obtains by equal-volume saturated ammonium sulfate aqueous solution, 4 DEG C stand 12 hours, and centrifugal 20 minutes of 8000rpm takes precipitation；Dissolve obtained precipitation by the mass ratio distilled water of 1:6, obtain lysate, be subsequently adding the caprylic acid relative to gained lysate 1.3 ‰, mixing；
(5) by the volume ratio of 1:2, saturated ammonium sulfate aqueous solution and step (4) are obtained lysate to mix, 4 DEG C stand 12 hours, centrifugal 20 minutes of 8000rpm, take precipitation, dissolve with PBS aseptic, pH7.2,0.01M by the mass ratio of 1:4, the solution 100Ku ultrafilter membrane bag obtained carries out desalination concentration, obtains concentrated solution；
(6) adding the formaldehyde of 0.1% in the concentrated solution that step (5) obtains, then room temperature stands 5 hours in sealed states, then with filtering with microporous membrane, obtains filtrate, is yolk antibody。
Comprise the yolk bioprotein skin repair dressing of described yolk bioprotein liquid, be made up of according to certain proportioning described yolk bioprotein liquid and sodium alginate, polyvinyl alcohol, chitosan, sorbitol, glycerol, compound vitamin, fibroin albumen。Described compound vitamin by the 1:1.2:2 mixture formed, is not only had the function of antioxidation, scavenging free radicals by vitamin A, vitamin C, vitamin E, moreover it is possible to promote epidermal growth。
Its preparation method is: add in described yolk bioprotein liquid relative to the sodium alginate of this yolk bioprotein liquid weight 1.6%, 5% polyvinyl alcohol, 5% chitosan, 0.5% sorbitol, 1.5% glycerol, 1% compound vitamin, 2% fibroin albumen, temperature of charge 50 DEG C is kept to be stirred, improve the degrees of fusion of material, stir, namely obtain described yolk bioprotein skin repair dressing。
Clinical verification:
50 volunteers are selected in the yolk bioprotein skin repair dressing that often group embodiment obtains respectively, totally 60, test, and volunteer is medical and beauty treatment postoperative skin the patient of wound, and allergy or facial infection occurs in postoperative face。At beauty and shaping wound surface smearing yolk bioprotein skin repair dressing, every day 2-3 time, routine observation, as used site of injury on the 15th unchanged continuously, it is invalid to be judged to, and stops treatment；Effective case continuous use 30 days, evaluates experimental result。
Select else and carry out the on probation of other products with 50 volunteers, as a control group。
Effect judgment criteria: effective: wound surface is without pain and gargalesthesia, and cicatrix is substantially thin out；Effective: wound surface pain and gargalesthesia alleviate, and cicatrix is thin out to some extent；Invalid: wound surface is unchanged。
Total effective rate %=(effective number of cases+effective number of cases)/total number of cases × 100%。
Following table is the experimental result of each embodiment。
| Group | Effective/people | Effectively/people | Invalid/people | Effective percentage/% |
| Embodiment 1 | 32 | 17 | 1 | 98 |
| Embodiment 2 | 31 | 16 | 3 | 94 |
| Embodiment 3 | 27 | 20 | 3 | 94 |
| Matched group | 6 | 35 | 9 | 82 |
Test result indicate that, the total effective rate of the present invention is more than or equal to 94%, and wound repair is respond well。In above-mentioned all of test volunteer, find no the volunteer of the ill symptomses such as skin irritant, red swelling of the skin, pruritus。
Conclusion: yolk bioprotein antibody anti-acne of the present invention, the anti-multiple antibacterial causing skin infection and microorganism。To medical cosmetic cosmesis rear face allergy, facial infection, cooperation epidermal growth factor reparation reduction wound, effect is notable。
In order to more intuitively express the effect of yolk bioprotein skin repair dressing of the present invention, select the typical clinical trial case of a few example and illustrate:
Mrs Lv, female, 36 years old, after cutting double eyelid, wound surface redness is not moved back, and wound is clearly, after the yolk bioprotein skin repair dressing of the use embodiment of the present invention 1 preparation 10 days, redness disappears, and wound surface is repaired gradually, it is continuing with after 20 days, wound surface is inconspicuous, close to normal skin, is continuing with after 30 days, heal completely, and parts cicatrix。
Mrs Zhong, 27 years old, after the micro-anaplasty of face, chin is red and swollen serious, pruritus even occurs, uses the yolk bioprotein skin repair casting product of the embodiment of the present invention 2 preparation after 15 days, redness is wholly absent, endoscope checks that internal wounds starts healing, is continuing with after 30 days, and wound surface recovers completely。
Mrs Lin, female, 42 years old, execute face's photon delicate skin postoperative, there is a wound local, and after using the yolk bioprotein skin repair dressing 12 days of the embodiment of the present invention 3 preparation, wound is recovered quickly, is continuing with to 20 days, and recovers completely。
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations。All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention。
Claims (7)
1. a yolk bioprotein liquid, it is characterized in that, by mass percentage, main component is as follows: multi-joint livetin 1-5%, epidermal growth factor 0.1-0.3%, surplus are hyaluronic acid solution, described multi-joint livetin is the pathogen of skin infection can be caused as immunogen using one or more, hen is carried out injecting immune, takes the egg of hen production and separate the yolk antibody that purification obtains。
2. a kind of yolk bioprotein liquid as claimed in claim 1, it is characterised in that described pathogen includes but not limited to propionibacterium acnes, leprosy bacillus, gonococcus, vermiform mite, acaricide。
3. a kind of yolk bioprotein liquid as claimed in claim 1, it is characterised in that the preparation method of described multi-joint livetin is:
(1) propionibacterium acnes leprosy bacillus, gonococcus, vermiform mite, acaricide are cultivated respectively by culture medium；The thalline obtained uses formalin-inactivated respectively, and oil emulsion inactivated vaccine is made in emulsifying；
(2) inactivated vaccine that hen obtains by above-mentioned steps (1) respectively being carried out immunity, the egg the separation that take hen production obtain yolk liquid；
(3) egg yolk liquid that step (2) obtains is mixed by the volume ratio of 1:1-5 with sterile purified water, 350-500rpm vibrates 15-30min, the volume ratio pressing 1:2 again adds sterile purified water, 600rpm vibrates 5-10min, regulate pH to 5.1,4 DEG C stand 10 hours, then centrifugal 10 minutes of 6000rpm, collect supernatant；
(4) adding in the supernatant that step (3) obtains by equal-volume saturated ammonium sulfate aqueous solution, 4 DEG C stand 12 hours, and centrifugal 20 minutes of 8000rpm takes precipitation；Dissolve obtained precipitation by the mass ratio distilled water of 1:6, obtain lysate, be subsequently adding the caprylic acid relative to gained lysate 1.3 ‰, mixing；
(5) by the volume ratio of 1:2, saturated ammonium sulfate aqueous solution and step (4) are obtained lysate to mix, 4 DEG C stand 12 hours, centrifugal 20 minutes of 8000rpm, take precipitation, dissolve with PBS aseptic, pH7.2,0.01M by the mass ratio of 1:4, the solution 100Ku ultrafilter membrane bag obtained carries out desalination concentration, obtains concentrated solution；
(6) adding the formaldehyde of 0.05-0.1% in the concentrated solution that step (5) obtains, then room temperature stands 3-5 hour in sealed states, then with filtering with microporous membrane, obtains filtrate, is multi-joint livetin。
4. a kind of yolk bioprotein liquid as claimed in claim 1, it is characterised in that the concentration of described hyaluronic acid solution is 6-8g/L。
5. the yolk bioprotein skin repair dressing of the yolk bioprotein liquid that one kind comprises described in claim 1, it is characterized in that, be made up of according to certain proportioning described yolk bioprotein liquid and sodium alginate, polyvinyl alcohol, chitosan, sorbitol, glycerol, compound vitamin, fibroin albumen。
6. a kind of yolk bioprotein skin repair dressing as claimed in claim 5, it is characterized in that, preparation method is: add in described yolk bioprotein liquid relative to the sodium alginate of this yolk bioprotein liquid weight 0.3-1.6%, the polyvinyl alcohol of 2-5%, the chitosan of 1-5%, the sorbitol of 0.1-0.5%, the glycerol of 1-1.5%, 0.2-1% compound vitamin, 0.3-2% fibroin albumen, stir, namely obtain described yolk bioprotein skin repair dressing。
7. the preparation method of a kind of yolk bioprotein skin repair dressing as claimed in claim 6, it is characterised in that keep temperature of charge to be 35-50 DEG C in described whipping process。
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| CN117599247A (en) * | 2023-12-14 | 2024-02-27 | 浙江来益美生物医药有限公司 | Silk fibroin microcarrier skin filling preparation, and preparation method and application thereof |
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| CN117599247A (en) * | 2023-12-14 | 2024-02-27 | 浙江来益美生物医药有限公司 | Silk fibroin microcarrier skin filling preparation, and preparation method and application thereof |
| CN117599247B (en) * | 2023-12-14 | 2024-05-31 | 浙江来益美生物医药有限公司 | Silk fibroin microcarrier skin filling preparation, and preparation method and application thereof |
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