CN105685031A - Application of cinnamic aldehyde and/or cinnamic aldehyde derivative in inhibition of aspergillus ochraceus growth - Google Patents
Application of cinnamic aldehyde and/or cinnamic aldehyde derivative in inhibition of aspergillus ochraceus growth Download PDFInfo
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- CN105685031A CN105685031A CN201610128609.6A CN201610128609A CN105685031A CN 105685031 A CN105685031 A CN 105685031A CN 201610128609 A CN201610128609 A CN 201610128609A CN 105685031 A CN105685031 A CN 105685031A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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Abstract
The invention discloses an application of cinnamic aldehyde and/or a cinnamic aldehyde derivative in inhibition of aspergillus ochraceus growth and further discloses an aspergillus ochraceus growth inhibitor. Active ingredients of the aspergillus ochraceus growth inhibitor include cinnamic aldehyde and/or the cinnamic aldehyde derivative. The cinnamic aldehyde and/or cinnamic aldehyde derivative resources are widely sourced in China, the production technology is simple, the cost is low, and the inhibitor has volatility; the inhibitor has good safety for people and livestock and has been listed in the food additive directory (GB 2760-2011 food security national standards-food additive usage standards); the inhibitor is pollution-free and has faint scent, and the period of validity can be up to one year or longer; the effect of the cinnamic aldehyde and/or the cinnamic aldehyde derivative applied to inhibition of aspergillus ochraceus growth is good, safety is achieved, and toxic and side effects are avoided.
Description
Technical field
The present invention relates to the application in suppressing Aspergillus ochraceus growth of a kind of cinnamic aldehyde and/or its derivant。
Background technology
Cinnamic aldehyde: English name, Cinnamicaldehyde, it is a kind of aldehydes organic compound, is present in a large number in the plants such as Cortex Cinnamomi。In nature, naturally occurring cinnamic aldehyde is transconfiguration, and this molecule is connect a phenyl on an acrylic aldehyde, therefore can be considered as a kind of Propenal derivative。Molecular formula is: C9H8O, molecular weight: 132.16, structural formula is as follows。
Aspergillus ochraceus (Aspergillusochraceus) is belonging to a kind of fungus of Eurotiale Trichocomaceae aspergillus, mainly pollute rice, Semen Tritici aestivi, Herba bromi japonici, coffee and beverage thereof etc., Aspergillus ochraceus pollution may result in above-mentioned food moulding and goes bad, food nutrition quality and processing quality significantly decline, and cause huge economic loss；More seriously Aspergillus ochraceus can produce the ochratoxin with strong toxicity and carcinogenecity in growth course, and ochratoxin has the compound that 7 kinds of structures are similar, and wherein ochratoxin A (OTA) toxicity is the strongest, is distributed the most extensive。In the mycotoxin having been found that, the hazardness of ochratoxin A (OTA) is only second to aflatoxin, and toxicologic study shows, OTA major determinant liver and kidney, having very strong hepatotoxicity and Toxicity of Kidney, substantial amounts of toxin is likely to and causes endo enteritis even downright bad；OTA is considered as one of the strongest natural materials of current carcinogenicity, is decided to be two grades of carcinogens by IARC。
Toxicity due to ochratoxin A (OTA), all disclose the limit standard of OTA in food and feedstuff both at home and abroad, produce with specification, control the pollution of OTA, ensure that the edible safety of humans and animals, table 1 list the limit standard of European Union, China and Taiwan ochratoxin to greatest extent。
The limit standard that table 1 European Union, China and TaiWan, China are leading to OTA in food
Therefore, screening and exploitation can efficiently suppress the natural active matter that Aspergillus ochraceus growth is safe and nontoxic, are subject to the pollution of Aspergillus ochraceus and ochratoxin for prevention and control food, promote food safety standard, have highly important economic worth and social meaning。
Summary of the invention
It is an object of the invention to provide the application in suppressing Aspergillus ochraceus growth of cinnamic aldehyde and/or its derivant。The inhibition that Aspergillus ochraceus is grown by cinnamic aldehyde of the present invention and/or its derivant is good。
The invention provides the application in suppressing Aspergillus ochraceus growth of cinnamic aldehyde and/or its derivant。
In above-mentioned application, described cinnamic aldehyde and/or its derivant suppress Aspergillus ochraceus growth described in following product: corn, food and/or beverage。
In above-mentioned application, it can be 100～1000 μ L/L that described cinnamic aldehyde and/or its derivant account for the concentration of described product, concretely 100 μ L/L, 200 μ L/L, 250 μ L/L, 300 μ L/L, 100～100 μ L/L or 100～500 μ L/L。
In above-mentioned application, stifling method is adopted to process described product；
The moisture of described corn and food can be all 0～15%, concretely 12%, 15%, 12～15% or 5～15%。
In above-mentioned application, the temperature of described suffocating treatment can be-10～37 DEG C, concretely 28 DEG C ,-10～28 DEG C, 28～37 DEG C or 10～35 DEG C；
The time of described suffocating treatment can be 3～45d, concretely 14d, 3～14d, 14～45d or 10～25d；
Described suffocating treatment carries out in closed system。
In above-mentioned application, described corn is at least one in Semen Tritici aestivi, Fructus Hordei Vulgaris, rice, Semen Maydis, rye (Secale cereale L.) and Herba bromi japonici；
Described food is at least one in coffee bean, Semen arachidis hypogaeae, Semen Juglandis, Fructus Pistaciae Verae, Semen coryli heterophyllae, Fructus anacardii, Semen Armeniacae Amarum, sunflower seed, fresh grape and dried Fructus Vitis viniferae；
Described beverage is at least one in Sucus Vitis viniferae, wine, coffee beverage, Fructus Hordei Germinatus beverage, alcoholic beverage, oat beverage and rice beverage。
Present invention also offers a kind of Aspergillus ochraceus growth inhibitor, its active component is cinnamic aldehyde and/or its derivant。
In the present invention, the method adopting cinnamic aldehyde and/or its derivant to suppress Aspergillus ochraceus growth, comprise the steps: the product of described cinnamic aldehyde and/or its derivant suffocating treatment, it is suppressed that the growth of described Aspergillus ochraceus。
The invention have the advantages that
Cinnamic aldehyde and/or its derivant resource are wide in China source, production technology is simple and easy, cost is low, have volatility；Person poultry safety's property is good, it is put into food additive catalogue (GB2760-2011 national food safety standard-food additive uses standard)；Environmentally safe, and have delicate fragrance, effect duration was up to more than 1 year。Cinnamic aldehyde and/or its derivant are applied to suppress the effective of Aspergillus ochraceus, safe without toxic side effect。
Accompanying drawing explanation
Fig. 1 is the impact that Aspergillus ochraceus is grown by variable concentrations cinnamic aldehyde。
Fig. 2 is that cinnamic aldehyde is stifling to the inhibition of Aspergillus ochraceus growth in Semen Tritici aestivi (moisture 12%)。
Fig. 3 is that cinnamic aldehyde is stifling to the inhibition of Aspergillus ochraceus growth in Semen Tritici aestivi (moisture 15%)。
Detailed description of the invention
The experimental technique used in following embodiment if no special instructions, is conventional method。
Material used in following embodiment, reagent etc., if no special instructions, all commercially obtain。
In following embodiment, the Aspergillus ochraceus (Aspergillusochraceus) of use is purchased from CGMCC, and this bacterial strain is numbered 3.4412 at CGMCC；The disclosure herein content and claim are all expressed as CGMCCNo.3.4412。
The stifling fungistatic effect of embodiment 1, cinnamic aldehyde measures
Cinnamic aldehyde: content >=98%。
PDA culture medium: peeled potatoes 200g takes filtrate after boiling 30min, adds glucose 20g, agar 16g, is settled to 1000mL with distilled water, adds deionized water and supply 1L, 115 DEG C of autoclavings 30 minutes, cools to about 55 DEG C and is down flat plate, every flat board 20mL。
MEA culture medium: yeast extract (1g/L), Fructus Hordei Germinatus extract (1g/L), peptone (1g/L), KH2PO4(1g/L)、MgSO4·7H2O(0.3g/L)、KCl(0.3g/L)、ZnSO4·7H2O(0.05g/L)、CuSO4·5H2O (0.01g/L), fructose (20g/L), agar (15g/L), add deionized water and supply 1L, 121 DEG C of autoclavings 20 minutes, cools to about 55 DEG C and is down flat plate, every flat board 20mL。
Actication of culture: Aspergillus ochraceus is inoculated in PDA culture medium, grows 14 days at 28 DEG C, 4 DEG C of storages。
Prepared by Aspergillus ochraceus spore liquid: be dissolved in 0.01% polysorbas20 with the Aspergillus ochraceus spore on aseptic cotton carrier picking plating medium, and diluting initial spore liquid to spore concentration with sterilized water is 1 × 105cfu/mL。
Aspergillus ochraceus bacteria suspension 10 μ L (1 × 105Cfu/mL) MEA culture medium central authorities it are inoculated in fully absorbing。By symmetrically placed in the edge of culture dish lid for sterilizing filter paper that 3 diameters are 10mm, cinnamic aldehyde 1,2,3,4,5 μ L respectively are in different culture dishs, so as to uniform adsorption is on filter paper, blank without cinnamic aldehyde, make the concentration range 20-100 μ L/L of cinnamic aldehyde, each do 3 parallel, culture dish sealed membrane is put in valve bag after sealing, it is placed in incubator 28 DEG C of cultivations, it is further cultured for 7 days after the flat board not having Aspergillus ochraceus to grow takes out cinnamic aldehyde after cultivating 20 days, observes the growth with or without mycelia。Within every 2 days, measure a colony diameter, adopt decussation method to measure twice, results averaged。It is shown that the cinnamic aldehyde of all fumigating concentrations all can significantly inhibit the growth of Aspergillus ochraceus, it is suppressed that effect strengthens along with the rising of cinnamic aldehyde concentration, can suppress the growth of Aspergillus ochraceus completely when cinnamic aldehyde fumigating concentration reaches 60 μ L/L。After taking out the cinnamic aldehyde scraps of paper the scattered taste that ventilates in fume hood, cultivate 7 days being put into incubator, have not yet to see mycelial growth, it was shown that cinnamic aldehyde spore is entirely killed。
Embodiment 2, cinnamic aldehyde are stifling suppresses Aspergillus ochraceus growth on Semen Tritici aestivi (moisture is 12%)
Cinnamic aldehyde: content >=98%。
Actication of culture and Aspergillus ochraceus spore liquid preparation method are with embodiment 1。
Wheat samples is through 1% liquor natrii hypochloritis's cleaning and sterilizing, and aseptic water washing, aseptic filter paper blots surface moisture。Add quantitative aseptic water according to presetting Target moisture difference, be put in 4 DEG C of standing 24h and make moisture balance, it is thus achieved that the Semen Tritici aestivi of the required moisture (12%) of experiment。Semen Tritici aestivi is dispensed in units of 200g in 1L conical flask, adds 1mL spore suspension (concentration 2 × 106Individual/mL), it is shaken gently for bottle and makes spore uniform fold to Semen Tritici aestivi。And then dropping cinnamic aldehyde is on aseptic filter paper, puts into conical flask, makes cinnamic aldehyde add concentration respectively 0,50,100,150 and 200 μ L/L, and after sealing, climatic chamber 28 DEG C cultivates 14d, measures pollution rate。Often group does 3 repetitions。Result shows, compared with the control, Aspergillus ochraceus pollution rate in Semen Tritici aestivi is all had significant reducing effect by all experimental concentration cinnamic aldehydes, and strengthen along with the rising of cinnamic aldehyde concentration, when cinnamic aldehyde concentration reaches 200 μ L/L, Semen Tritici aestivi pollution rate reduces to 0, it was shown that the cinnamic aldehyde of 200 μ L/L can suppress Aspergillus ochraceus infecting Semen Tritici aestivi completely。
Embodiment 3, cinnamic aldehyde are stifling suppresses Aspergillus ochraceus growth on Semen Tritici aestivi (moisture is 15%)
Cinnamic aldehyde: content >=98%。
Actication of culture and Aspergillus ochraceus spore liquid preparation method are with embodiment 1。
Wheat samples is through 1% liquor natrii hypochloritis's cleaning and sterilizing, and aseptic water washing, aseptic filter paper blots surface moisture。Add quantitative aseptic water according to presetting Target moisture difference, be put in 4 DEG C of standing 24h and make moisture balance, it is thus achieved that the Semen Tritici aestivi of the required moisture (15%) of experiment。Semen Tritici aestivi is dispensed in units of 200g in 1L conical flask, adds 1mL spore suspension (concentration 2 × 106Individual/mL), it is shaken gently for bottle and makes spore uniform fold to Semen Tritici aestivi。And then dropping cinnamic aldehyde is on aseptic filter paper, puts into conical flask, makes cinnamic aldehyde add concentration respectively 0,100,150,200 and 250 μ L/L, and after sealing, climatic chamber 28 DEG C cultivates 14d, measures pollution rate。Often group does 3 repetitions。Result shows, compared with the control, Aspergillus ochraceus pollution rate in Semen Tritici aestivi is all had significant reducing effect by all experimental concentration cinnamic aldehydes, and strengthen along with the rising of cinnamic aldehyde concentration, when cinnamic aldehyde concentration reaches 250 μ L/L, Semen Tritici aestivi pollution rate reduces to 0, it was shown that the cinnamic aldehyde of 250 μ L/L can suppress Aspergillus ochraceus infecting Semen Tritici aestivi completely；The Aspergillus ochraceus pollution rate of the moisture 15% Semen Tritici aestivi Semen Tritici aestivi apparently higher than 12%。
Claims (7)
1. cinnamic aldehyde and/or the application in suppressing Aspergillus ochraceus growth of its derivant。
2. application according to claim 1, it is characterised in that: described cinnamic aldehyde and/or its derivant suppress Aspergillus ochraceus growth described in following product: corn, food and/or beverage。
3. application according to claim 2, it is characterised in that: it is 100～1000 μ L/L that described cinnamic aldehyde and/or its derivant account for the concentration of described product。
4. the application according to Claims 2 or 3, it is characterised in that: adopt stifling method to process described product；
The moisture of described corn and food is 0～15%。
5. application according to claim 4, it is characterised in that: the temperature of described suffocating treatment is-10～37 DEG C；
The time of described suffocating treatment is 3～45d；
Described suffocating treatment carries out in closed system。
6. the application according to any one of claim 2-5, it is characterised in that: described corn is at least one in Semen Tritici aestivi, Fructus Hordei Vulgaris, rice, Semen Maydis, rye (Secale cereale L.) and Herba bromi japonici；
Described food is at least one in coffee bean, Semen arachidis hypogaeae, Semen Juglandis, Fructus Pistaciae Verae, Semen coryli heterophyllae, Fructus anacardii, Semen Armeniacae Amarum, sunflower seed, fresh grape and dried Fructus Vitis viniferae；
Described beverage is at least one in Sucus Vitis viniferae, wine, coffee beverage, Fructus Hordei Germinatus beverage, alcoholic beverage, oat beverage and rice beverage。
7. an Aspergillus ochraceus growth inhibitor, it is characterised in that: the active component of this inhibitor is cinnamic aldehyde and/or its derivant。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610128609.6A CN105685031A (en) | 2016-03-08 | 2016-03-08 | Application of cinnamic aldehyde and/or cinnamic aldehyde derivative in inhibition of aspergillus ochraceus growth |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112868652A (en) * | 2021-01-29 | 2021-06-01 | 浙江工业大学 | Cinnamaldehyde-containing emulsion for inhibiting growth of aspergillus ochraceus and preparation method thereof |
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Non-Patent Citations (1)
| Title |
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| 滑慧娟: "挥发性植物精油抑制赭曲霉生产及产毒的研究", 《中国优秀硕士学位论文全文数据库 工程科技I 辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112868652A (en) * | 2021-01-29 | 2021-06-01 | 浙江工业大学 | Cinnamaldehyde-containing emulsion for inhibiting growth of aspergillus ochraceus and preparation method thereof |
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Application publication date: 20160622 |