CN105669807A - Vitamin B12 fermentation liquid separation, purification and concentration technique - Google Patents
Vitamin B12 fermentation liquid separation, purification and concentration technique Download PDFInfo
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- CN105669807A CN105669807A CN201610129799.3A CN201610129799A CN105669807A CN 105669807 A CN105669807 A CN 105669807A CN 201610129799 A CN201610129799 A CN 201610129799A CN 105669807 A CN105669807 A CN 105669807A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H23/00—Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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Abstract
The invention relates to a vitamin B12 fermentation liquid separation, purification and concentration technique which comprises the following steps: 1. treating the vitamin B12 fermentation liquid by using a ceramic membrane system, and adding water for elution in the treatment process to remove high-molecular proteins and suspended substances; 2. sending the treated ceramic membrane dialysate into an ultrafiltration membrane system to separate small molecule proteins and vitamin B12, and adding water for elution in the separation process, thereby obtaining an ultrafiltration concentrated solution and an ultrafiltration dialysate; 3. sending the ultrafiltration dialysate into a nanofiltration system to perform concentration, thereby obtaining a nanofiltration concentrated solution and a nanofiltration dialysate; and 4. sending the nanofiltration concentrated solution into a continuous ion exchange system, and carrying out exchange adsorption on the vitamin B12 through a cation exchange resin, thereby obtaining a vitamin B12 resolution solution. The technique abandons the plate and frame pressure filtration and the fixed bed, so that the overall water consumption, chemical consumption and organic solvent consumption are greatly lowered, thereby lowering the production cost and enhancing the production benefits.
Description
Technical field
The present invention relates to vitamin B12 preparation field, in particular to a kind of vitamin B12 separation of fermentative broth, purification, concentration technology.
Background technology
Vitamin B12 is again cobalami, is the VITAMIN uniquely containing metallic element. Vitamin B12 in nature is all Microbe synthesis, and high animals and plants can not manufacture vitamin B12. A kind of intestinal secretion thing (castle's intrinsic factor) of a kind of needs that vitamin B12 is only helps the VITAMIN that could be absorbed. The main Physiological Function of vitamin B12 participates in manufacturing erythrocyte, prevents pernicious anemia; Cerebral nerve is prevented to be damaged. Vitamin B12 finds one the latest in vitamin B group up to now. Vitamin B12 is a kind of many compound ring series containing 3 valency cobalts, and the pyrrole ring of 4 reduction connects together and is turned into 1 the big ring of corrin (similar to porphyrin), is the core of vitamin B12 molecule. So the compound containing this kind of ring is all called as class corrin. Vitamin B12 is the needle crystal of light red, soluble in water and ethanol, under pH value 4.5～5.0 mild acid conditions the most stable, strong acid (pH < 2) or basic solution decompose, heat can have to a certain degree to be destroyed, but the high-temperature sterilization of short period of time loss is little, chance high light or ultraviolet are easily destroyed, common gastronomical process loss amount about 30%.
As shown in Figure 1, in existing technique, vitamin B12 fermented liquid generally adopts filter press to process in conjunction with the mode of fixed bed in treating processes. Owing to mycelial density is higher, it is easy to cause plate frame flow little, it is easy to blocking. And the medium consumption existed in fixed bed concentration process is big, output and the problem such as product concentration is low, pharmaceutical chemicals, eluting agent are big.
Summary of the invention
It is an object of the invention to provide one can efficient recovery water resources, reduce concentration process medium amount, improve output, product concentration, reduce pharmaceutical chemicals and eluting agent, reduce enterprise's production cost, it is to increase the vitamin B12 separation of fermentative broth of income, purification, concentration technology.
For achieving the above object, the technical scheme that the present invention proposes is: a kind of vitamin B12 separation of fermentative broth, purification, concentration technology, it is characterised in that, comprise the steps:
Step 1: use ceramic membrane system to be processed by vitamin B12 fermented liquid, add water in treating processes and carry out wash-out, remove high molecular weight protein and suspended substance;
Step 2: ceramic membrane dialyzate enters ultrafiltration membrane system and is separated with vitamin B12 by small molecular protein after treatment, adds water and carries out wash-out, obtain ultrafiltration and concentration liquid and ultrafiltration dialysis liquid in sepn process;
Step 3: ultrafiltration dialysis liquid enters nanofiltration system and concentrates, obtaining nanofiltration concentrated solution is and nanofiltration dialyzate;
Step 4: nanofiltration concentrated solution enters continuous ionic exchange system, carries out exchanging absorption to vitamin B12 by Zeo-karb, obtains vitamin B12 desorbed solution.
Further, ceramic membrane aperture described in step 1 is 20-100nm, and service temperature less than 70 DEG C, selective penetrated property is more than 96%, it is preferable to 50nm.
Further, the multiple that adds water described in step 1 is 2 times, and the control total nitrogen of feed liquid is below 0.4%.
Further, ultra-filtration membrane molecular weight described in step 2 is 10000-50000 dalton, and working pressure controls in 2Bar, PVDF material, and molecular weight cut-off is preferably 30000 dalton.
Further, the multiple that adds water described in step 2 is 0.1 times.
Further, described vitamin B12 fermented liquid is the nutrient solution cultivating vitamin B12 mycelium.
Further, also comprise and it is used in step 1 and step 2 by the nanofiltration dialyzate described in step 3 adding water in process to use water.
Further, Zeo-karb described in step 4 is weakly acidic cationic exchange resin of acrylic series.
Adopt technique scheme, vitamin B12 separation of fermentative broth of the present invention, purification, concentration technology, the useful effect having is: make vitamin B12 reach separation, purification, concentrated object, abandon filter press and fixed bed, overall water consumption, chemical article, organic solvent usage quantity are reduced greatly, thus reach production cost, it is to increase production benefit.
Accompanying drawing explanation
Fig. 1 is prior art vitamin B12 separation of fermentative broth, purification, concentration technology schema;
Fig. 2 is the vitamin B12 separation of fermentative broth described in invention, purification, concentration technology schema.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
Embodiment
Vitamin B12 separation of fermentative broth of the present invention, purification, concentration technology schema are as shown in Figure 1;
Step 1: use ceramic membrane system to be processed by vitamin B12 fermented liquid, remove the macro-molecular protein in feed liquid and mycelium, ensure the steady running of follow-up system; Ceramic membrane aperture is 50nm, service temperature less than 70 DEG C, and selective penetrated property is more than 96%; Carry out three batches of experiments:
From experimental data, high receipts rate that ceramic membrane system has, remove the features such as protein content height.
Step 2: use ultrafiltration to be processed by ceramic membrane dialyzate, will wherein be separated with vitamin B12 by small molecular protein. Ultra-filtration membrane molecular weight cut-off is 30000 dalton, and working pressure controls in 2Bar, PVDF material, and control temperature is at 40 DEG C; Carry out three batches of experiments:
From experimental data, being retained by small molecular protein by ultrafiltration, in feed liquid, protein content has reached few standard, and ultrafiltration has the feature such as good separating effect, disintegrate-quality height.
Step 3: use nanofiltration to be processed by ultra-filtration membrane dialyzate, will wherein concentrate by vitamin B12 so that it is reach stock liquid standard. Control temperature at 40 DEG C, pressure 20Bar. Carry out three batches of experiments:
From experimental data, it may also be useful to ultrafiltration dialysis liquid is concentrated by nanofiltration system, nanofiltration concentrated solution vitamin B12 content all can exceed stock liquid, reaches continuously from the charging standard handed over. And nanofiltration dialyzate can return ceramic membrane and ultrafiltration system as wash-out water, vitamin B12 does not cause loss.
Step 4: use and adsorbed by nanofiltration concentrated solution from friendship continuously, adopts weakly acidic cationic exchange resin of acrylic series, and control charging content is about about 200ug/ml, has altogether carried out the experiment of three batches:
From upper table experimental data, the just calculation receipts rate of three batches of experiments, all more than 100%, gets rid of the error on weighing, it can be seen that product does not lose substantially, and the content of desorbed solution is all at more than 1800ug/ml, and feed liquid is concentrated about 9 times by entirety.
Claims (10)
1. a vitamin B12 separation of fermentative broth, purification, concentration technology, it is characterised in that: comprise the steps:
Step 1: use ceramic membrane system to be processed by vitamin B12 fermented liquid, add water in treating processes and carry out wash-out, remove high molecular weight protein and suspended substance;
Step 2: ceramic membrane dialyzate enters ultrafiltration membrane system and is separated with vitamin B12 by small molecular protein after treatment, adds water and carries out wash-out, obtain ultrafiltration and concentration liquid and ultrafiltration dialysis liquid in sepn process;
Step 3: ultrafiltration dialysis liquid enters nanofiltration system and concentrates, obtaining nanofiltration concentrated solution is and nanofiltration dialyzate;
Step 4: nanofiltration concentrated solution enters continuous ionic exchange system, carries out exchanging absorption to vitamin B12 by Zeo-karb, obtains vitamin B12 desorbed solution.
2. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 1, it is characterised in that: ceramic membrane aperture described in step 1 is 20-100nm, and service temperature less than 70 DEG C, selective penetrated property is more than 96%.
3. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 2, it is characterised in that: ceramic membrane aperture described in step 1 is 50nm.
4. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 1, it is characterised in that: the multiple that adds water described in step 1 is 2 times, and the control total nitrogen of feed liquid is below 0.4%.
5. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 1, it is characterised in that: ultra-filtration membrane molecular weight cut-off described in step 2 is 10000-50000 dalton, and working pressure controls in 2Bar, PVDF material.
6. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 5, it is characterised in that: ultra-filtration membrane molecular weight cut-off described in step 2 is 30000 dalton.
7. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 1, it is characterised in that:, the multiple that adds water described in step 2 is 0.1 times.
8. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 1, it is characterised in that: described vitamin B12 fermented liquid is the nutrient solution cultivating vitamin B12 mycelium.
9. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 1, it is characterised in that: also comprise and it is used in step 1 and step 2 by the nanofiltration dialyzate described in step 3 adding water in process to use water.
10. a kind of vitamin B12 separation of fermentative broth, purification, concentration technology according to claim 1, it is characterised in that: the Zeo-karb described in step 4 is weakly acidic cationic exchange resin of acrylic series.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107915749A (en) * | 2016-10-11 | 2018-04-17 | 伊犁川宁生物技术有限公司 | A kind of method of purification of cephalosporin C zymotic fluids |
| CN109402187A (en) * | 2018-11-15 | 2019-03-01 | 沈阳农业大学 | Agua-Mephyton 2 and thrombus dissolving enzyme co-production |
| CN109776641A (en) * | 2019-03-21 | 2019-05-21 | 山东泓达生物科技有限公司 | A kind of extraction process of vitamin B12 product |
| CN113083253A (en) * | 2021-04-08 | 2021-07-09 | 陕西蓝深特种树脂有限公司 | Weak acid cation resin for extracting vitamin B12 and synthetic method thereof |
| CN113583070A (en) * | 2021-08-05 | 2021-11-02 | 安徽普朗膜技术有限公司 | Separation and purification method for extracting vitamin VB12 from fermentation liquor |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107915749A (en) * | 2016-10-11 | 2018-04-17 | 伊犁川宁生物技术有限公司 | A kind of method of purification of cephalosporin C zymotic fluids |
| CN109402187A (en) * | 2018-11-15 | 2019-03-01 | 沈阳农业大学 | Agua-Mephyton 2 and thrombus dissolving enzyme co-production |
| CN109776641A (en) * | 2019-03-21 | 2019-05-21 | 山东泓达生物科技有限公司 | A kind of extraction process of vitamin B12 product |
| CN113083253A (en) * | 2021-04-08 | 2021-07-09 | 陕西蓝深特种树脂有限公司 | Weak acid cation resin for extracting vitamin B12 and synthetic method thereof |
| CN113083253B (en) * | 2021-04-08 | 2023-04-04 | 陕西蓝深特种树脂有限公司 | Weak acid cation resin for extracting vitamin B12 and synthetic method thereof |
| CN113583070A (en) * | 2021-08-05 | 2021-11-02 | 安徽普朗膜技术有限公司 | Separation and purification method for extracting vitamin VB12 from fermentation liquor |
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Application publication date: 20160615 |