CN105663195B - A kind of extracting method of ginsenoside - Google Patents
A kind of extracting method of ginsenoside Download PDFInfo
- Publication number
- CN105663195B CN105663195B CN201610236817.8A CN201610236817A CN105663195B CN 105663195 B CN105663195 B CN 105663195B CN 201610236817 A CN201610236817 A CN 201610236817A CN 105663195 B CN105663195 B CN 105663195B
- Authority
- CN
- China
- Prior art keywords
- ginsenoside
- ethanol
- extract
- extraction
- ginseng
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
- B01J31/02—Catalysts comprising hydrides, coordination complexes or organic compounds containing organic compounds or metal hydrides
- B01J31/0201—Oxygen-containing compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Steroid Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of general ginsenoside rich in ginseng sapoglycoside Rg 3 and its extracting method.Ginsenoside Rb1, Rb2, Rb3, RC, Rg3 mass percent are respectively in the extract：1.0% 3.0%, 0.2% 1.0%, 0.5 2.5%, 0.5% 2.5%, 28% 40%.This method comprises the following steps：Ginseng is extracted using microwave loss mechanisms using ethanol as Extraction solvent, extracts in solution and adds a certain amount of vitamin C, the crude extract of general ginsenoside is obtained by the extracting through microwave heating of certain time；Using macroreticular resin absorbing method, further enriching and purifying obtains general ginsenoside extract to the crude extract of general ginsenoside.
Description
Technical field
The present invention relates to a kind of ginsenoside and its extracting method, in particular, it is related to a kind of rich in the rare soap of ginseng
The ginsenoside and its extracting method of glycosides, it is especially a kind of to make use of being rich in for Catalyzed by Vitamin C technology and microwave heating method
The rare saponin(e Rg of ginseng3General ginsenoside extracting method.
Background technology
Ginseng (Panax ginseng C.A.Mey) is the root and rhizome of araliaceae ginseng plant's ginseng, first recorded in《God
Agriculture book on Chinese herbal medicine warp》, top grade is classified as, is traditional rare medicinal herbs, the king in medicine is described as, with reinforcing vital energy, prevent prolapse is promoted the production of body fluid, is pacified
Effect of god.Cure mainly the deficiency of vital energy to be intended to take off, internal lesion caused by overexertion is deficient, burnout, indigestion and loss of appetite, vomit, lingering diarrhea of defecating, shortness of breath, spontaneous perspiration, chronic cough and dyspnea of deficiency type disappears
Yearningly, insomnia, palpitates with fear, forgetful, impotence, frequent micturition, the card of all deficiency of vital energy Tianjin wounds such as uterine bleeding.It is used as traditional rare Chinese medicine, ginseng tool
There are a variety of definite pharmacological actions, such as anti-aging, antifatigue, anti-oxidant, antitumor, anti-diabetic.
Chemical composition contained by ginseng includes ginsenoside, carbohydrate, amino acid, vitamin, protein, polypeptide, organic acid, fat
Soluble components and trace element etc..Modern medicine proves, ginsenoside is a mostly important class physiologically active thing in ginseng
Matter, with regulation central nervous system, improve cardiovascular and hemopoietic system, regulation internal system, improve immunity, it is antifatigue
And the effect such as anticancer.Monomer saponin kind more than 40 is had been found that in ginseng, its content accounts for the 4% of ginseng dry mass, and ginseng
Total saponin content accounts for the 2.0%-7.0% of dry mass in main root.Ginsenoside is the triterpene being formed by connecting by sugar chain and aglycon
Class compound.Triterpenoids sapogenins saponin(e and dammarane type four-ring triterpenoid saponin are divided into according to saponin(e structure.With up to agate
Alkane type tetracyclic triterpene saponin(e being characterized property composition, dammarane type four-ring triterpenoid saponin is different according to aglycone structure, can be divided into protoplast
Join diol type, such as ginsenoside Rb1、Rb2, RC and Rd；Protopanaxatriol type, such as ginsenoside Re, Rf, Rg1、Rg2Deng.
Research shows, the rare ginsenoside obtained by the selective hydrolysis of sugared aglucon contained main ginseng than in ginseng
Saponin(e has more preferable body absorbability and pharmacological activity.Rare ginsenoside and aglycon have more preferable activity, are improving
Effect is unique in terms of memory and treatment and prevention of tumour.The absorption of the natural ginseng saponin(e of rich content in the gastrointestinal tract is very poor.
Blood concentration is extremely difficult to give full play to the concentration of pharmacological activity in body.Saponin(e needs could quilt after degraded de-glycosylation
Absorption of human body is utilized.Diol type saponin(e could enter blood after being hydrolyzed into rare saponin(e.As can be seen here, the generation of ginsenoside
Thanking product-secondary saponin and aglycon has stronger activity, is more easy to be absorbed by organisms, and plays drug effect.It is a large amount of prepare secondary glycosides and
Aglycon has obvious medical value and market value.
Ginseng sapoglycoside Rg 3 is by former ginsenoside glycol saponins Rb1、Rb2、Rb3, Rc, Rd etc. C-20 positions glycosyl
Obtained secondary rare ginsenoside is hydrolyzed, remembers with anticancer, Anti-tumor metastasis, neuroprotection, immunity enhancing, improvement, resist
The curative effects such as pressure, anti-wrinkle, in addition ginsenoside Rg3Use, can effectively improve with other cancer therapy drugs or chemotherapy drugs in combination
Therapeutic effect, the life quality of raising people to cancer.In the prior art, ginsenoside Rg3Can by ginsenoside acid,
It is prepared by the catalyzing hydrolysis such as alkali, enzyme.Sour water solution is ginsenoside Rg3The method being commonly used in preparation, mainly utilizes hydrochloric acid, sulphur
Acid, acetic acid, formic acid, tartaric acid etc. are used as catalyst.But, above-mentioned inorganic acid is easily caused excessive hydrolysis due to acid strong, and right
The corrosivity of equipment is strong and the shortcomings of there is spent acid discharge, is unfavorable for the production of industrialization.Formic acid is acid appropriate but volatile,
There is infringement to human body, environment, tartaic acid is weaker, be difficult to remove.
For a long time, people are directed to finding a kind of more suitable for industrialization production, can extract and obtain from ginseng
High activity, low sugar base ginsenoside method.At present, method used to PDS mostly by carrying out enzyme hydrolysis
Or acid-hydrolyzed method prepares ginsenoside, have not seen direct by the optimization of extraction conditions in the extraction process of ginsenoside
Promote the method for the generation of the rare saponin(e of ginseng, use vitamin C as catalyst to hydrolyze acquisition rich in dilute while having not seen
There is ginsenoside Rg3Ginsenoside extracting method.
The content of the invention
It is an object of the invention to solve above-mentioned technical problem, a kind of operation letter is provided by a series of condition optimizing
Just, production cost is low, production efficiency is high is rich in ginsenoside Rg3General ginsenoside extracting method, extraction process use
Microwave heating method, catalyst is used as by the use of vitamin C.
The present invention develops new catalyst vitamin C, using vitamin C as catalyst general ginsenoside extraction
During, diol saponin is directly converted into rare ginsenoside Rg3, people is rich in notable physiologically active so as to obtain
Join saponin(e Rg3General ginsenoside extract.Using vitamin C as catalyst, reaction condition is gentle, does not corrode instrument and equipment,
Environment will not be polluted.Vitamin C is harmless and necessary to being human body, and intake in appropriate amount is beneficial to maintain body
Every function of organization, therefore, in the post processor of extract, for ascorbic separation without excessively strict, this
Point make it that extraction process is more simple and easy to do, with wide market application foreground.
The technical scheme is that：
One kind is rich in ginsenoside Rg3General ginsenoside extract, it is characterised in that ginsenoside in the extract
Rb1、Rb2、Rb3、RC、Rg3Mass percent be respectively：1.0%-3.0%, 0.2%-1.0%, 0.5-2.5%, 0.5%-
2.5%th, 28%-40%.
One kind is rich in ginsenoside Rg3General ginsenoside extract preparation method, it is characterised in that include following step
Suddenly：
(1) ginseng is extracted using microwave loss mechanisms using ethanol as Extraction solvent, extracts in solution and add necessarily
The vitamin C of amount, the crude extract of general ginsenoside is obtained by the extracting through microwave heating of certain time；
(2) using macroreticular resin absorbing method, further enriching and purifying obtains general ginsenoside to the crude extract of general ginsenoside
Extract.
Concentration of alcohol described in step (1) is 50%-80%, preferably 60%-70%；It is ascorbic in extract solution
Concentration is 0.6-2.0g/100ml；The consumption of ethanol is 8-20 times (ml/g) of ginseng weight.
Ascorbic concentration is preferably 1.2g/100ml in step (1) extract solution.
The consumption of ethanol is preferably 15 times (ml/g) of ginseng weight in step (1).
It is preferred that, the power of Microwave Extraction is 200-600W, more preferably 300W.
It is preferred that Microwave Extraction time be 15-30min, more preferably 20min.
In step (2), the macroreticular resin of absorption saponin(e is eluted respectively with deionized water, ethanol, collect ethanol eluate, subtract
Pressure concentration, dry reaction product, i.e. general ginsenoside.
The volume of deionized water used is 3-6BV, and the volume of ethanol is 5-7BV, and concentration is 50%-70%.
Ginsenoside is soluble in water, methanol, ethanol, dissolves in n-butanol, acetic acid and ethyl acetate, insoluble in chloroform, second
In ether, benzene.Ethanol is nontoxic, cheap, impurity content is few in extract solution, and present invention selection ethanol is molten as the extraction of ginsenoside
Agent.Influence of the concentration of alcohol change to ginsenoside extraction efficiency, the content of total saposins in 70% ethanol extract are investigated
Highest, is then gradually reduced, and the preferred concentration of ethanol is 50%-80%.
Microwave be wavelength in the range of 1mm to 1m (frequency 300-300000MHz) electromagnetic wave, positioned at infrared radiating light and
Between radio wave, the most frequently used heating frequency is 2450MHz.It has been generally acknowledged that heating of the medium in microwave field has two kinds of machines
Reason, i.e. ion conduction mechanism and dipole rotate mechanism.Microwave auxiliary extraction technology is microwave heating and traditional solvent extraction
A kind of new extractive technique that method is formed after being combined, makes analyte be separated from sample matrices and enters using microwave heating
Solvent, the otherness of microwave ability is absorbed according to the selection of appropriate solvent and different compounds, raising target compound is reached
A kind of Novel extraction method of extraction efficiency.Compared with traditional extraction process, microwave―assisted extraction have extraction time it is short, choosing
The advantages of selecting property is good, the rate of recovery is high, reagent dosage is few.
The degree that different material absorbs microwave in the electromagnetic field of microwave radiation is different, the electricity of ethanol, water in microwave radiation
Material by microwave application is absorbed in magnetic field, microwave radiation energy is converted into heat energy, and then transfers heat to other materials coexisted, from
And compared with traditional heating mode, significantly shorten the time of extraction.
Microwave power and extraction time have been investigated, said extracted condition has been optimized, to obtain maximum extracted production
Rate.As a result indicate, during from 70% ethanol water (V/V) as Extraction solvent, the power of Microwave Extraction is preferably 200-
600W, more preferably 300w；The time of Microwave Extraction is preferably 15-30 minutes, more preferably 20 minutes.
Vitamin C is added during Microwave Extraction as catalyst, ascorbic consumption has been investigated to ginsenoside
Rg3The influence of yield.
Quantitative analysis is carried out to ginsenoside Rb1, Rb2, Rb3, RC, Rg3 by high performance liquid chromatography.Chromatographic condition is such as
Under：Lunna NH2Chromatographic column (4.6mm × 150mm, 5 μm)；Mobile phase acetonitrile-water gradient；Flow velocity 1.0mLmin-1；Inspection
Survey wavelength 203nm；Column temperature：30℃；The μ L of sample size 20.Condition of gradient elution is：Mobile phase acetonitrile (A)：Water (B), gradient elution
(0-20min, A:25→40；20-33min, A:40→60；33-37min, A:46；37-45min, A:46→50；45-62min,
A:50→70；62-65min, A:70→90).
Embodiment
Embodiment 1
Single factor exploration
With the recovery rate of general ginsenoside (quality of general ginsenoside accounts for the percentage of sample gross mass i.e. in extract)
Single factor exploration is carried out to concentration of alcohol, consumption, microwave abstracting number of times as index.Obtain the optimum level of each single factor test
Respectively concentration of alcohol 70% (V/V), consumption is 15 times (ml/g) of ginseng weight, and extraction times are 2 times.
Orthogonal experiment
To optimize the experiment condition of extracting through microwave heating, in the feelings that above-mentioned concentration of alcohol, consumption, extraction times condition are constant
Under condition, microwave power (A) is selected, vitamin C concentration (B), extraction time (C) is used as investigation factor (as shown in table 1), Mei Geyin
Element takes three levels, and orthogonal experiment is carried out according to table 2.The extract of acquisition uses macroreticular resin ginseng therein
Total saposins, after the elution impurity elimination of 5BV deionized waters, are enriched with general ginsenoside, and use high-efficient liquid phase technique with the ethanol of 7BV 60%
The content (W/W) of the Rg3 in general ginsenoside is determined, three factors are analyzed.R values in table 2, it can be seen that each
Factor is respectively B to the influence degree of microwave auxiliary extraction ginsenoside recovery rate>A>C, ascorbic concentration influence is most aobvious
Write, microwave power takes second place, the influence of extraction time is minimum.It is preferred that scheme be A2B2C2, i.e. microwave power is 300w, vitamin C
Concentration is 1.2 (g/100ml), and extraction time is 20 minutes.
The L of table 19(34) orthogonal test factor and level
The orthogonal experiments of table 2
The content analysis of ginsenoside composition in the extract of embodiment 2
Ginsenoside extract A preparation：2.5 grams of samples of Ginseng are weighed, are surpassed at room temperature with 50ml70% (V/V) ethanol
Sound is extracted 2 times, and 60 minutes every time, extract solution is recovered under reduced pressure into solvent under the conditions of 40 DEG C, is concentrated as the aqueous solution, is inhaled using macropore
Attached resin concentration purifies general ginsenoside, elutes impurity elimination first with 5BV deionized waters, is then enriched with ginseng with the ethanol of 7BV 60%
Total saposins, carry out the purifying of saponin(e, elution speed is 1.5ml/min.Ethanol eluate is collected, rotary evaporation removes ethanol in proper amount
Afterwards, concentrate is placed in stainless steel disc, in being evaporated in water-bath, 60 DEG C are dried under reduced pressure, and obtain ginsenoside extract A.
Ginsenoside extract B preparation：2.5 grams of samples of Ginseng are weighed, are placed in the extractor of Microwave Extraction Apparatus, are added
(V/V) ethanol solutions of 50ml 70% and 0.6 gram of vitamin C, are 300W by extraction power setting, and extraction time is 20 minutes,
After the completion of extraction, collect in filtered fluid, filter residue and add (V/V) ethanol solutions of 50ml 70% and 0.6 gram of vitamin C again, together
The extraction conditions of sample collects filtrate after the completion of extracting.Filtrate it will merge twice, rotary evaporation to volume is about 20ml, uses concentrated ammonia liquor
PH value is adjusted to 7.0, using D101 enriching and purifying macroporous resin general ginsenosides, is gone first with the elution of 5BV deionized waters
It is miscellaneous, general ginsenoside is then enriched with the ethanol of 7BV 60%, the purifying of saponin(e is carried out, elution speed is 1.5ml/min.Collect second
Alcohol eluen, rotary evaporation is removed after ethanol in proper amount, and concentrate is placed in stainless steel disc, and in being evaporated in water-bath, 60 DEG C depressurize
Dry, obtain ginsenoside extract B.
Using ginsenoside Rb in ginsenoside extract A, B obtained by high effective liquid chromatography for measuring1, ginsenoside Rb2、
Ginsenoside Rb3, ginsenoside RC, ginsenoside Rg3Mass percent.
The preparation of mixed reference substance solution：Precision weighs ginsenoside Rb1Reference substance, ginsenoside Rb2Reference substance, ginseng
Saponin(e Rb3Reference substance, ginsenoside RC reference substances and ginsenoside Rg3In right amount, it is accurately weighed, it is placed in same 5ml measuring bottles, uses
Methanol dissolving and constant volume, are made mixed reference substance solution.
The preparation of need testing solution：
Ginsenoside extract A about 30mg, accurate Cheng Ding are taken, is placed in 10ml measuring bottles, plus methanol dissolving and constant volume, shake
It is even, 0.45 μm of filter membrane is crossed, it is continuous to take filtrate, as need testing solution A；
Ginsenoside extract B about 30mg, accurate Cheng Ding are taken, is placed in 10ml measuring bottles, plus methanol dissolving and constant volume, shake
It is even, 0.45 μm of filter membrane is crossed, it is continuous to take filtrate, as need testing solution B.
Chromatographic condition：Lunna NH2Chromatographic column (4.6mm × 150mm, 5 μm)；Mobile phase acetonitrile-water gradient；Flow velocity
1.0mL·min-1；Detection wavelength 203nm；Column temperature：30℃；The μ L of sample size 20.Condition of gradient elution is：Mobile phase：Acetonitrile (A)：
Water (B), gradient elution (0-20min, A:25→40；20-33min, A:40→60；33-37min, A:46；37-45min, A:46
→50；45-62min, A:50→70；62-65min, A:70→90).
As a result：The content for determining ginsenoside composition in ginsenoside extract A groups, B groups is as shown in table 3, it is seen then that dimension life
Plain C addition and the use of microwave auxiliary extraction method are during the extract of ginsenoside effectively by panoxadiol group
Saponin(e is converted into rare ginsenoside Rg3.
Ginsenoside component content (weight/mass percentage composition) in the extract of table 3
Embodiment 3
Weigh 3 grams of samples of Ginseng, be placed in the extractor of Microwave Extraction Apparatus, add (V/V) ethanol solutions of 45ml 70% with
And 0.9 gram of vitamin C, it is 350W by extraction power setting, extraction time is 30 minutes, after the completion of extraction, collects filtered fluid, filter
(V/V) ethanol solutions of 45ml 70% and 0.6 gram of vitamin C are added in slag again, same extraction conditions is received after the completion of extracting
Collect filtrate.It filtrate will merge twice, rotary evaporation to volume is about 20ml, and adjust pH value to 7.0 with concentrated ammonia liquor, inhaled using macropore
Attached resin concentration purifies general ginsenoside, elutes impurity elimination first with 4BV deionized waters, is then enriched with ginseng with the ethanol of 9BV 60%
Total saposins, carry out the purifying of saponin(e, elution speed is 1.5ml/min.Ethanol eluate is collected, rotary evaporation removes ethanol in proper amount
Afterwards, concentrate is placed in stainless steel disc, in being evaporated in water-bath, 60 DEG C are dried under reduced pressure, and obtain ginsenoside extract.With reference to implementation
Ginsenoside Rb in ginsenoside extract obtained by the high effective liquid chromatography for measuring of example 21, ginsenoside Rb2, ginsenoside
Rb3, ginsenoside RC, ginsenoside Rg3Mass percent, as a result as shown in table 4.
Ginsenoside component content (weight/mass percentage composition) in the extract of table 4
Embodiment 4
Weigh 5 grams of samples of Ginseng, be placed in the extractor of Microwave Extraction Apparatus, add (V/V) ethanol solutions of 80ml 60% with
And 1.0 grams of vitamin Cs, it is 600W by extraction power setting, extraction time is 30 minutes, after the completion of extraction, collects filtered fluid, filter
Add (V/V) ethanol solutions of 60ml 60% and 0.6 gram of vitamin C in slag again, extraction power is 600W, extraction time is
20 minutes, filtrate is collected after the completion of extraction.Filtrate it will merge twice, rotary evaporation to volume is about 30ml, is adjusted with concentrated ammonia liquor
PH value using enriching and purifying macroporous resin general ginsenoside, elutes impurity elimination to 7.0 with 6BV deionized waters first, then with
The ethanol of 8BV 60% is enriched with general ginsenoside, carries out the purifying of saponin(e, elution speed is 1.5ml/min.Collect ethanol eluate,
Rotary evaporation is removed after ethanol in proper amount, concentrate is placed in stainless steel disc, in being evaporated in water-bath, 60 DEG C are dried under reduced pressure, and obtain people
Join saponin extract.Ginsenoside Rb in the ginsenoside extract with reference to obtained by the high effective liquid chromatography for measuring of embodiment 21、
Ginsenoside Rb2, ginsenoside Rb3, ginsenoside RC, ginsenoside Rg3Mass percent, as a result as shown in table 5.
Ginsenoside component content (weight/mass percentage composition) in the extract of table 5
Claims (1)
1. one kind uses vitamin C as catalyst preparation and is rich in ginsenoside Rg3General ginsenoside extract method, its
It is characterised by comprising the steps of：
（1）Ginseng is extracted using microwave loss mechanisms using 60%-70% ethanol as Extraction solvent, the consumption of ethanol is ginseng
8-20 times of weight, wherein ethanol consumption are in terms of milliliter, and ginseng weight is in gram；It is 1.2g/ to extract and concentration is added in solution
100ml vitamin C, the crude extract of general ginsenoside, the work(of Microwave Extraction are obtained by 15-30min extracting through microwave heating
Rate is 300W, and the time of Microwave Extraction is 15-30min；
（2）Using macroreticular resin absorbing method, further enriching and purifying obtains general ginsenoside extraction to the crude extract of general ginsenoside
Thing；Elute the macroreticular resin of absorption saponin(e respectively with deionized water, ethanol, collect ethanol eluate, be concentrated under reduced pressure, it is anti-dry
Product, i.e. general ginsenoside are answered, the volume of deionized water used is 3-6BV, and the volume of ethanol is 5-7BV, and concentration is 50%-
70%；
Extract obtained middle ginsenoside Rb1、Rb2、Rb3、RC、Rg3Mass percent be respectively：1.0%-3.0%、0.2%-
1.0%、0.5-2.5%、0.5%-2.5%、28%-40%。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610236817.8A CN105663195B (en) | 2016-04-18 | 2016-04-18 | A kind of extracting method of ginsenoside |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610236817.8A CN105663195B (en) | 2016-04-18 | 2016-04-18 | A kind of extracting method of ginsenoside |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105663195A CN105663195A (en) | 2016-06-15 |
| CN105663195B true CN105663195B (en) | 2017-07-25 |
Family
ID=56309902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610236817.8A Active CN105663195B (en) | 2016-04-18 | 2016-04-18 | A kind of extracting method of ginsenoside |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105663195B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107095879A (en) * | 2017-04-19 | 2017-08-29 | 韩春超 | A kind of antineoplastic containing protopanoxadiol |
| CN107779475B (en) * | 2017-09-30 | 2021-06-15 | 安徽利民生物科技股份有限公司 | Extraction method of luffa saponin |
| CN108912202B (en) * | 2018-05-28 | 2021-06-11 | 江苏苏南药业实业有限公司 | Method for extracting ginsenoside |
| CN109651481A (en) * | 2018-12-27 | 2019-04-19 | 湖州市食品药品检验研究院 | A kind of ginsenoside extracting method and its application |
| CN110577561B (en) * | 2019-09-20 | 2023-07-11 | 四川省原子能研究院 | A method for preparing ginsenoside Rg by irradiation 3 Is a method of (2) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100706111B1 (en) * | 2005-07-12 | 2007-04-12 | 고성권 | A ginseng preparation using vitamin c and process for thereof |
-
2016
- 2016-04-18 CN CN201610236817.8A patent/CN105663195B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 人参中人参皂苷的提取、分离和测定;王玉堂;《中国博士学位论文全文数据库工程科技Ⅰ辑》;20081115(第11期);第18、60页 * |
| 大孔树脂吸附人参总皂苷工艺及再生使用的研究;张崇禧等;《中国药学杂志》;20030930;第38卷(第9期);第661-664页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105663195A (en) | 2016-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105663195B (en) | A kind of extracting method of ginsenoside | |
| CN101863871B (en) | Total glycosides of Rhodiola rosea, medical application and preparation method thereof | |
| CN107271577A (en) | A kind of analysis of effective component method of stilbene Siberian cocklebur warm kidney medicine for eliminating bursa | |
| CN102352402B (en) | Method for preparing red ginseng saponins Rg3 group and Rh2 group mixed saponins | |
| CN104415070A (en) | Method for separating and purifying total ginsenoside from ginseng | |
| CN106727806B (en) | A kind of preparation method of sanchi leaf total saposins | |
| CN107510710A (en) | A kind of method and medical usage that diabetes B target spot inhibitor is enriched with from Glycyrrhiza uralensisFisch residue | |
| CN107823237A (en) | A kind of extracting method of burdock root total flavone | |
| CN108670973A (en) | A kind of Chloranthus glaber anti-influenza virus activity extract and preparation method thereof | |
| Li et al. | Rapid characterization of the constituents in Jigucao capsule using ultra high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry | |
| CN105017345B (en) | The method of four kinds of compounds of extraction and the application simultaneously from selfheal | |
| CN105832789A (en) | Method for preparing saponin enriched product through high-pressure microwave assisted semi-bionic extraction | |
| CN108530500A (en) | A kind of preparation method and applications of cortex albiziae lignan glycosides monomer | |
| CN104958330A (en) | Oroxylum indicum general flavone extraction and purification method and application thereof | |
| CN104873570B (en) | A kind of method for extraction and purification of Prunella vulgaris general flavone and its application | |
| CN104069145B (en) | Application of the eupatorium lindleynun var. trifoliolatum in anti-hepatic-B virus medicine is prepared | |
| CN105601693B (en) | Ginseng saponin F1Preparation and its antitumor action | |
| CN115453035B (en) | Method for extracting high-content notoginsenoside R1 from pseudo-ginseng | |
| CN103083388B (en) | Preparation method of fructus gleditsiae total saponins | |
| CN107320511A (en) | Microwave conversion notoginseng haulm saponin(e prepares method and the application of active component | |
| US10435384B2 (en) | Method for extracting herbacetin from plants of Rhodiola L | |
| CN114591390A (en) | Preparation method of gypenoside | |
| CN109336942B (en) | Method for extracting purified panax japonicus saponin VI from ginseng | |
| CN105616470A (en) | Method for extracting and separating saponin and polysaccharide at the same time from brown ginger | |
| CN112656828A (en) | Pseudo-ginseng leaf product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |