CN105661507A - Method for producing inulin, natto and enzyme powder through solid state fermentation - Google Patents

Method for producing inulin, natto and enzyme powder through solid state fermentation Download PDF

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Publication number
CN105661507A
CN105661507A CN201610025207.3A CN201610025207A CN105661507A CN 105661507 A CN105661507 A CN 105661507A CN 201610025207 A CN201610025207 A CN 201610025207A CN 105661507 A CN105661507 A CN 105661507A
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natto
powder
inulin
fermentation
hours
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CN201610025207.3A
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Chinese (zh)
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王军
王淼
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Individual
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention provides a method for producing inulin, natto and enzyme powder through solid state fermentation. The inulin, natto and enzyme powder is prepared from the following components in a compounding ratio: 48-69% of natto and 40-60% of inulin. The method comprises the specific operation steps of firstly performing natto extraction, then performing inulin extraction, and finally mixing natto powder with inulin. The inulin, natto and enzyme powder is synbiotics consisting of the inulin and the natto powder, and is scientifically composited with natural enzymes; the formula is in scientific proportion compatibility, so that the comprehensive recuperation effects of probiotics, prebiotics and enzymes to gastrointestinal tracts are fully exerted, and the gastroenteric functions can be comprehensively and efficiently restored. The inulin, natto and enzyme powder is the only synbiotic product containing the natto powder at present, the natto powder can be efficiently expressed in human bodies, and the effects of the natto powder on restraining harmful bacteria and enabling beneficial bacteria to be proliferated are irreplaceable by other strains. Various raw materials and ingredients contained in the inulin, natto and enzyme powder are all 100% natural pure powder, and the purposes of zero addition and zero pollution are really realized.

Description

A kind of solid fermentation produces the method for inulin natto enzyme powder
Technical field
The present invention relates to the use of inulin, natto, particularly relate to a kind of solid fermentation and produce inulin nattoFermentThe method of powder.
Background technology
Inulin is that a class is naturalLevanMixture, levan be fructose units pass through (2-1) chain attachment form and withGlucoseThe carbohydrate that unit terminates, in usual commercialization inulin, the average degree of polymerization of levan is 10-30, wherein contains a small amount ofOligofructoseCan finding the existence of inulin in almost all of plant, it is the form of the another kind of energy storage of plant except starch, and we extract from Jerusalem artichoke plant and refine and obtain inulin product, the degree of polymerization from the mixture of more than 2 ~ 60 kinds of levan, is very good functionalFood ingredient, simultaneously be also produce oligofructose,High fructose syrupFructoseVery good material Deng product.InulinIt it is high-qualityPrebioticsWithDietary fiber, it is possible toThe intestines and stomach coordinating, clear up enterotoxin, clear up blood fat, reduceBlood glucose, looks improving and the skin nourishing, improves resistance etc. Natto powder by Japan Xu Jian foreign firm in 1987 from Japan traditional food natto powder find since, natto powder is conducted extensive research by people, has isolated pure enzyme, has found in an experiment, natto powder can absorb in digested road, and creates thrombolytic effect in vivo. Natto powder can thrombus, have and prevent osteoporosis, regulate the intestines and stomach, loosening bowel to relieve constipation, the effect such as eliminating toxin and beautifying the skin. But effect ratio of simple natto powder and simple inulin is single now, and during use, health-care effect is undesirable.
Summary of the invention
According to above technical problem, the present invention provides a kind of solid fermentation to produce inulin nattoFermentThe method of powder, its concrete proportioning is: natto 48-69%, inulin 40-60%; Its concrete operation step is:
(1) first carry out natto extraction, first carry out solid fermentation: selected Semen Glycines flowing water is soaked 20 hours, steaming and decocting 30 minutes under 0.1Mpa; After cooling, natto powder being inoculated, inoculum concentration is 1%, in 37 DEG C of bottom fermentations 18 hours after inoculation; By stand-by for the natto homogenizing that ferments; Carrying out liquid fermentation after solid fermentation: preparation culture medium, wherein take Semen sojae atricolor powder 12%, glucose 7.2%, dextrin 4.8%, NaCl0.4% by weight percentage, surplus is water, and pH is 7.0;Natto powder is inoculated, and inoculum concentration is 5%; Fermentation, in 37 DEG C of bottom fermentations 24 hours after inoculation; Ferment complete, take fermentation liquid stand-by; Flat band method and fiber flat band method is used to determine nattokinase vigor and the Bafillus natt viable count of above-mentioned solid fermentation product and liquid fermentation production, by solid fermentation product and liquid fermentation production by the ratio of g/ml, mix homogenizing final vacuum lyophilization with the ratio of 1:9 and obtain the natto powder of required nattokinase vigor;
(2) then carry out inulin extraction, remove the impurity of Jerusalem artichoke, clean with clear water, remove the peel, cut into slices, dry, pulverize, obtain 50 order~90 order dry powder; Described dry powder is dissolved in water, then adopts microwave extracting to extract; Wherein, the mass ratio of described water and described dry powder is 9~10:1, and described microwave extracting power is 500 watts~550 watts, and the microwave extracting time is 3 minutes~5 minutes; Hot water extraction 40 minutes~60 minutes, obtains crude extract; Wherein, the temperature of described hot water is 70 DEG C~90 DEG C; Described crude extract is passed into exchange resin tower and carries out desolventing technology, obtain the Jerusalem artichoke solution refined; Wherein, bleaching time is 2 hours~2.5 hours; Described refining Jerusalem artichoke solution is carried out concentrating under reduced pressure; Inulin concentrated solution is carried out spray drying, obtains inulin powder product;
(3) finally natto powder and inulin are mixed, the natto powder and the inulin powder that produce above-mentioned steps weigh raw material according to the ratio of 48-69:40-60, then the raw material weighed is pulverized respectively, then vibrosieve is utilized to screen, after screening, both are mixed, during mixing, raw material is put in mixer together and make it be sufficiently mixed.
The invention have the benefit that inulin nattoFermentThe symphysis unit that powder is made up of inulin, natto powder, carries out science compound with natural ferment, and this prescription, through science ratio compatibility, has given full play to probiotic bacteria, prebiotics, ferment to gastrointestinal tract comprehensively regulating effect, it is possible to recover gastrointestinal function efficiently comprehensively. Inulin natto enzyme powder is the currently the only symphysis unit product containing natto powder, natto powder can in human body high efficient expression, it suppresses harmful bacteria, and the effect of propagation probiotics is otherStrainInstitute is irreplaceable. Inulin nattoFermentVarious material compositions contained by powder are 100% natural pure powder, are truly realized zero interpolation, no pollution.
Detailed description of the invention
According to embodiment, the present invention is further described:
Embodiment 1
A kind of solid fermentation produces inulin nattoFermentThe method of powder, its concrete operation step is:
(1) first carry out natto extraction, first carry out solid fermentation: selected Semen Glycines flowing water is soaked 20 hours, steaming and decocting 30 minutes under 0.1Mpa; After cooling, natto powder being inoculated, inoculum concentration is 1%, in 37 DEG C of bottom fermentations 18 hours after inoculation; By stand-by for the natto homogenizing that ferments; Carrying out liquid fermentation after solid fermentation: preparation culture medium, wherein take Semen sojae atricolor powder 12%, glucose 7.2%, dextrin 4.8%, NaCl0.4% by weight percentage, surplus is water, and pH is 7.0; Natto powder is inoculated, and inoculum concentration is 5%; Fermentation, in 37 DEG C of bottom fermentations 24 hours after inoculation; Ferment complete, take fermentation liquid stand-by; Flat band method and fiber flat band method is used to determine nattokinase vigor and the Bafillus natt viable count of above-mentioned solid fermentation product and liquid fermentation production, by solid fermentation product and liquid fermentation production by the ratio of g/ml, mix homogenizing final vacuum lyophilization with the ratio of 1:9 and obtain the natto powder of required nattokinase vigor;
(2) then carry out inulin extraction, remove the impurity of Jerusalem artichoke, clean with clear water, remove the peel, cut into slices, dry, pulverize, obtain 50 order~90 order dry powder; Described dry powder is dissolved in water, then adopts microwave extracting to extract; Wherein, the mass ratio of described water and described dry powder is 9~10:1, and described microwave extracting power is 500 watts~550 watts, and the microwave extracting time is 3 minutes~5 minutes; Hot water extraction 40 minutes~60 minutes, obtains crude extract; Wherein, the temperature of described hot water is 70 DEG C~90 DEG C; Described crude extract is passed into exchange resin tower and carries out desolventing technology, obtain the Jerusalem artichoke solution refined; Wherein, bleaching time is 2 hours~2.5 hours; Described refining Jerusalem artichoke solution is carried out concentrating under reduced pressure; Inulin concentrated solution is carried out spray drying, obtains inulin powder product;
(3) natto powder and the inulin powder that finally above-mentioned steps are produced weigh raw material according to the ratio of 48:52, then the raw material weighed is pulverized respectively, then vibrosieve is utilized to screen, after screening, both are mixed, during mixing, raw material is put in mixer together and make it be sufficiently mixed.
The above is only the preferred embodiment of the present invention, it is noted that for those skilled in the art, under the premise without departing from the principles of the invention, it is also possible to make some improvement, and these improvement also should be regarded as protection scope of the present invention.

Claims (1)

1. a solid fermentation produces inulin nattoFermentThe method of powder, its concrete proportioning is: natto 48-69%, inulin 40-60%; Its concrete operation step is:
(1) first carry out natto extraction, first carry out solid fermentation: selected Semen Glycines flowing water is soaked 20 hours, steaming and decocting 30 minutes under 0.1Mpa; After cooling, natto powder being inoculated, inoculum concentration is 1%, in 37 DEG C of bottom fermentations 18 hours after inoculation; By stand-by for the natto homogenizing that ferments; Carrying out liquid fermentation after solid fermentation: preparation culture medium, wherein take Semen sojae atricolor powder 12%, glucose 7.2%, dextrin 4.8%, NaCl0.4% by weight percentage, surplus is water, and pH is 7.0; Natto powder is inoculated, and inoculum concentration is 5%; Fermentation, in 37 DEG C of bottom fermentations 24 hours after inoculation; Ferment complete, take fermentation liquid stand-by; Flat band method and fiber flat band method is used to determine nattokinase vigor and the Bafillus natt viable count of above-mentioned solid fermentation product and liquid fermentation production, by solid fermentation product and liquid fermentation production by the ratio of g/ml, mix homogenizing final vacuum lyophilization with the ratio of 1:9 and obtain the natto powder of required nattokinase vigor;
(2) then carry out inulin extraction, remove the impurity of Jerusalem artichoke, clean with clear water, remove the peel, cut into slices, dry, pulverize, obtain 50 order~90 order dry powder; Described dry powder is dissolved in water, then adopts microwave extracting to extract; Wherein, the mass ratio of described water and described dry powder is 9~10:1, and described microwave extracting power is 500 watts~550 watts, and the microwave extracting time is 3 minutes~5 minutes; Hot water extraction 40 minutes~60 minutes, obtains crude extract; Wherein, the temperature of described hot water is 70 DEG C~90 DEG C; Described crude extract is passed into exchange resin tower and carries out desolventing technology, obtain the Jerusalem artichoke solution refined; Wherein, bleaching time is 2 hours~2.5 hours; Described refining Jerusalem artichoke solution is carried out concentrating under reduced pressure; Inulin concentrated solution is carried out spray drying, obtains inulin powder product;
(3) finally natto powder and inulin are mixed, the natto powder and the inulin powder that produce above-mentioned steps weigh raw material according to the ratio of 48-69:40-60, then the raw material weighed is pulverized respectively, then vibrosieve is utilized to screen, after screening, both are mixed, during mixing, raw material is put in mixer together and make it be sufficiently mixed.
CN201610025207.3A 2016-01-15 2016-01-15 Method for producing inulin, natto and enzyme powder through solid state fermentation Pending CN105661507A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107692047A (en) * 2017-09-29 2018-02-16 潍坊茜月制药技术有限公司 A kind of preparation method of natto functional food
CN107897901A (en) * 2017-12-01 2018-04-13 高州市客多多农产品开发有限公司 A kind of black soya bean enzyme film
CN108523001A (en) * 2018-01-05 2018-09-14 辽宁亿通宝生物科技有限公司 A kind of natto powder and its production technology
CN109845974A (en) * 2018-11-07 2019-06-07 马学军 A kind of preparation method of natto entire body

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005077349A1 (en) * 2004-02-17 2005-08-25 Otsuka Pharmaceutical Co., Ltd. HUMAN β-DEFENSIN PRODUCTION ACCELERATOR
CN101816395A (en) * 2009-11-12 2010-09-01 天津市百奥生物技术有限公司 Process for producing natto powder
CN104585766A (en) * 2015-02-05 2015-05-06 广州赛莱拉干细胞科技股份有限公司 Composition as well as preparation method and application thereof
CN104650264A (en) * 2014-06-26 2015-05-27 钟延华 Method for extracting inulin

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005077349A1 (en) * 2004-02-17 2005-08-25 Otsuka Pharmaceutical Co., Ltd. HUMAN β-DEFENSIN PRODUCTION ACCELERATOR
CN101816395A (en) * 2009-11-12 2010-09-01 天津市百奥生物技术有限公司 Process for producing natto powder
CN104650264A (en) * 2014-06-26 2015-05-27 钟延华 Method for extracting inulin
CN104585766A (en) * 2015-02-05 2015-05-06 广州赛莱拉干细胞科技股份有限公司 Composition as well as preparation method and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
流水: "如何才能保持年轻态", 《大家健康》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107692047A (en) * 2017-09-29 2018-02-16 潍坊茜月制药技术有限公司 A kind of preparation method of natto functional food
CN107897901A (en) * 2017-12-01 2018-04-13 高州市客多多农产品开发有限公司 A kind of black soya bean enzyme film
CN108523001A (en) * 2018-01-05 2018-09-14 辽宁亿通宝生物科技有限公司 A kind of natto powder and its production technology
CN109845974A (en) * 2018-11-07 2019-06-07 马学军 A kind of preparation method of natto entire body

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