CN105660907A

CN105660907A - Method for preparing camellia oleifera abel or camellia oleifera abel flower honey enzyme drink through nectar source bacterium fermentation

Info

Publication number: CN105660907A
Application number: CN201610140156.9A
Authority: CN
Inventors: 廖祥儒; 李韵雅; 孟天添; 江威
Original assignee: Jiangnan University
Current assignee: Jiangnan University
Priority date: 2016-03-11
Filing date: 2016-03-11
Publication date: 2016-06-15

Abstract

The invention provides a method for preparing a camellia oleifera abel or camellia oleifera abel flower honey enzyme drink through nectar source bacterium fermentation.The method is technically characterized in that camellia oleifera abel or camellia oleifera abel flower is taken as the main material, and bacillus amyloliquefaciens saved in China Center for Type Culture Collection on June 24, 2013 with the preservation number of CCTCC M2013283 is used as the bacterium carrier culture medium of fermentation strain.Honey and water are added into a fermentation tank with the ratio of primary pulp to honey to water being (4-6) : (1-3) : (5-1) during fermentation, then nectar source bacterium freeze-dried powder is added, PH is maintained to be 5-8, temperature is 20-35 DEG C, and rotating speed is 220 rpm, wherein the weight of nectar source bacterium freeze-dried powder is 0.01-0.05% of the total weight of primary pulp, honey and water.Air agitation is conducted in the fermentation tank for fermentation lasting 7-15 days, and obtained fermentation liquor is the garden stuff honey enzyme drink.

Description

A kind of method of nectar source bacterium fermentation preparation oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage

Technical field

The strain of a kind of fermentable oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. beverage and method, be specifically related to a kind of have, without what pathogen or other microorganisms were polluted, the method that tea beverage is prepared in the fermentation improving local flavor. Belong to technical field of bioengineering.

Background technology

Tea oil tree is one of big woody oleiferous plants in the world four, and it is grown in the high mountain of south China subtropical zone and hilly country is the distinctive a kind of senior oil plant of pure natural of China. It is concentrated mainly on Hunan, Jiangxi, Fujian, Zhejiang, Hubei, Anhui, Guangxi, Guizhou, Guangdong, Sichuan etc. to economize, only Hunan yield just accounts for whole nation tea-oil tree yield more than 40%, the tea-oil tree yield in Hunan and Jiangxi adds up and accounts for the whole nation 70%, promote in oil tea plantation situation energetically recent years in country, the newly-increased area of camellia oleifera lam is with the speed increment of annual ten thousand mu, and China's oil plant safety is significant. Oil tea and Elaeis guineensis Jacq., Fructus oleae europaeae and Cortex cocois radicis are also called the big woody edible oil tannin plant in the world four. The unsaturated fatty acid content of Oleum Camelliae, up to 90%, is significantly larger than vegetable oil, Oleum Arachidis hypogaeae semen and Oleum Glycines, doubles than content of vitamin E with olive oil, and containing specific biological active substanceies such as camellianins, has high nutritive value. Oil tea has significantly high comprehensive utilization value, containing tea saponin, Camellia Oleifera polysaccharide, camellia oleosa seed albumen etc. in leached tea oil slag, they are all the raw materials of chemical industry, light industry, food, feed industry product etc., and tea seed episperm may also be fabricated which furfural, activated carbon etc., Camellia oleifera Abel or a kind of good culture medium of edible fungus. Research shows, oil tea saponin also has antibacterial and antioxidation.

The medical value of oil tea: root bark (Cortex Camelliae Oleiferae Radicis): bitter is flat. Slightly poisonous. Removing blood stasis and activating blood flow, synthetism is subsided a swelling. For fracturing, bruise, stomachache, skin pruritus, burn. Flower (Flos Camelliae): bitter, cold. Micro-poison. Cooling blood for hemostasis. For gastrointestinal hemorrhage, hemoptysis, epistaxis, discharging fresh blood stool, metrorrhagia; Outer for burn. Seed (Semen Camelliae): bitter is flat. Poisonous. Circulation of qi promoting is dredged stagnant. For abdominal pain due to stagnation of QI, have loose bowels, skin pruritus, burn. Seed fat oil (Oleum Camelliae): sweet is flat. Clearing away heat and eliminating dampness, parasite killing detoxifies. Suffer from abdominal pain for eruptive disease, acute ascarid obstructive intestinal obstruction, scabies, burn.Seed residue (Residuum Camelliae Semen preparatum) after oil expression: pungent, bitter, puckery. Slightly poisonous. Removing dampness parasite killing.

Mel is a kind of supersaturation fructose soln, and water activity is low, and osmotic pressure is high, and containing a small amount of H₂O₂And the polyphenol substance of variable concentrations and other antioxidant factors, have anticancer, anti-aging effect. Therefore the ability that nectar source bacterium generally has a stronger osmophilic strain and high oxidation is coerced. Bacillus in more existing Mel is as the report of probiotic bacteria. What this patent related to sieve from Mel, deposit number is: the bacillus amyloliquefaciens of the BacillusamyloliquefaciensH47 of CCTCCM2013283 has good bioconversion and produces the ability of Antioxidative Factors.

Summary of the invention:

The present invention prepares oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. beverage by additional single strain fermentation, to improve tea leaf quality, promotes millet paste mouthfeel, and can alleviate the health hazard that oil tea raw material toxic composition brings. Fermentation stage can generate multiple flavor substance, can produce and accumulate a large amount of metabolite, generate the multiple beneficial materials such as organic acid, enzyme, sugar alcohol, Polyphenols, the nutrition contained in conjunction with raw material and microorganism self and functional component, to facilitating digestion, strengthen radioprotective immune, anticancer, slow down aging etc. human body have multiple beneficial effect. This approach avoid the harm of the infective pathogen bacterium such as easy infection escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa in the original sweat of Folium Camelliae sinensis, it is possible to provide there is the tea in existing mouthfeel, and the economic worth of oil tea can be increased, improve tea grower's income.

Technical scheme:

The purpose of the present invention is to propose to a kind of tea beverage preparation method, it is without expensive device, can be prevented effectively from fungus and the generation of pathogenic bacterium toxin, and can stablize and persistently have good health-care effect.

With on June 24th, 2013 be stored in the deposit number of Wuhan, China Wuhan University China typical culture collection center for: the bacillus amyloliquefaciens of the BacillusamyloliquefaciensH47 of CCTCCM2013283 is fermenting and producing starting strain, utilizes oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. fermentation to prepare tea beverage. Being divided into two stages, the fermentation of first stage is the biotransformation of bacillus amyloliquefaciens, drying to remove moisture and assorted taste of second stage. Implementing process is:

1) select without going rotten rotten fresh or dried Camellia Leaves or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. water rinses out surface contaminants, soak 15 minutes in 1% 2% aqueous alkalis, drain surface moisture after rinsed clean, break into oleo stock. Pass into fermentation tank stand-by.

2) according to oleo stock, Mel, water 4～6:1～3:5～1 ratio add Mel and water to fermentation tank after add the deposit number of three kinds of raw material gross weights 0.01% 0.05% and be: the BacillusamyloliquefaciensH47 nectar source bacterium lyophilized powder of CCTCCM2013283, mix homogeneously in the lump.

3) PH=5～8, temperature 20 35 DEG C, rotating speed 220rpm are kept. Air agitation aerobic fermentation 7 15 days in fermentation tank.

4) fermentation liquid after fermenting 7 15 days is oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage.

Beneficial effects of the present invention:

The invention provides a kind of tea beverage with health-care effect. In preparation method of the present invention and process, non-pollution safety is reliable, and the fermentation time cycle is short, and process procedure is few, simple to operation, can reduce investment outlay and operating cost. The present invention can effectively prevent miscellaneous bacteria from growing, and additional strain can bring added health effect, makes macromolecular substances resolve into rapidly the small-molecule substance that human body easily absorbs, and effectively puies forward various health-care effecies and the high added value of tea beverage.

Detailed description of the invention:

Below in conjunction with embodiment, the present invention is described further.Following example are only several specific embodiments of the present invention, but the design concept of the present invention is not limited thereto, all changes utilizing this design that the present invention carries out unsubstantiality, all should belong to the behavior invading scope.

Embodiment 1

Select green oil Folium Camelliae sinensis 1 kilogram, soak 15 minutes in 1% aqueous alkali, drain surface moisture after rinsed clean, break into underflow. It is added thereto to 0.2 kilogram of Mel, after 0.8 kilogram of mineral water, adds the nectar source bacterium BacillusamyloliquefaciensH47 lyophilized powder of 0.8g, keep PH=6, temperature 22 DEG C, rotating speed 220rpm. Air agitation aerobic fermentation 12 days in fermentation tank. Obtain taste flavor tool good, the Folium Camelliae sinensis enzyme beverage that health-care effect is good.

Embodiment 2

Select green oil Flos Camelliae Japonicae 1 kilogram, soak 15 minutes in 1% aqueous alkali, drain surface moisture after rinsed clean, break into underflow. It is added thereto to 0.2 kilogram of Mel, after 0.8 kilogram of mineral water, adds the nectar source bacterium BacillusamyloliquefaciensH47 lyophilized powder of 0.8g, keep PH=6, temperature 22 DEG C, rotating speed 220rpm. Air agitation aerobic fermentation 12 days in fermentation tank. Obtain taste flavor tool good, the Flos Camelliae Japonicae enzyme beverage that health-care effect is good.

Embodiment 3

Select market to sell the dry tea of oil tea 1 kilogram, soak 15 minutes in 1% aqueous alkali, drain surface moisture after rinsed clean, break into underflow. It is added thereto to 0.2 kilogram of Mel, after 0.8 kilogram of mineral water, adds the nectar source bacterium BacillusamyloliquefaciensH47 lyophilized powder of 0.8g, keep PH=6, temperature 22 DEG C, rotating speed 220rpm. Air agitation aerobic fermentation 12 days in fermentation tank. Obtain taste flavor tool good, the tea liquid enzyme beverage that health-care effect is good.

Embodiment 4

Select oil tea dry flower 1 kilogram, soak 15 minutes in 1% aqueous alkali, drain surface moisture after rinsed clean, break into underflow. It is added thereto to 0.2 kilogram of Mel, after 0.8 kilogram of mineral water, adds the nectar source bacterium BacillusamyloliquefaciensH47 lyophilized powder of 0.8g, keep PH=6, temperature 22 DEG C, rotating speed 220rpm. Air agitation aerobic fermentation 12 days in fermentation tank. Obtain taste flavor tool good, the Flos Camelliae Japonicae enzyme beverage that health-care effect is good.

Claims

1. by nectar source bacterium fermentation preparation oil tea or the method for Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage, being characterized in, with oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. for raw material, being aided with a small amount of culture medium, preparing tea beverage by nectar source bacterium fermentation, the method comprises the following steps:

A) select without going rotten rotten fresh or dried Camellia Leaves or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. water rinses out surface contaminants, soak 15 minutes in 1% 2% aqueous alkalis, drain surface moisture after rinsed clean, break into oleo stock. Pass into fermentation tank stand-by.

B) according to oleo stock, Mel, water 4～6:1～3:5～1 ratio add Mel and water to fermentation tank after add the deposit number of three kinds of raw material gross weights 0.01% 0.05% and be: the BacillusamyloliquefaciensH47 nectar source bacterium lyophilized powder of CCTCCM2013283, mix homogeneously in the lump.

C) PH=5～8, temperature 20 35 DEG C, rotating speed 220rpm are kept. Air agitation aerobic fermentation 7 15 days in fermentation tank.

D) fermentation liquid after fermenting 7 15 days is oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage.

2. the method for a kind of nectar source according to claim 1 bacterium fermentation preparation oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage, is characterized in that: bacillus amyloliquefaciens refers to the bacillus cereus sieved from natural honey.

3. the method for a kind of nectar source according to claim 1 bacterium fermentation preparation oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage, is characterized in that: fermentation strain adopts the Bacillus amyloliquefaciens strain of the BacillusamyloliquefaciensH47 that deposit number is CCTCCM2013283 being stored in China typical culture collection center on June 24th, 2013 to be fermentation strain.

4. the method for a kind of nectar source according to claim 1 bacterium fermentation preparation oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage, it is characterized in that: oleo stock, Mel, water 4～6:1～3:5～1 ratio add Mel and water to fermentation tank after add the deposit number of three kinds of raw material gross weights 0.01% 0.05% and be: the BacillusamyloliquefaciensH47 nectar source bacterium lyophilized powder of CCTCCM2013283, mix homogeneously in the lump.

5. a kind of nectar source according to claim 1 bacterium fermentation preparation oil tea or the method for Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage, is characterized in that: the addition of natural honey is 10% 30%.

6. the method for a kind of nectar source according to claim 1 bacterium fermentation preparation oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage, it is characterized in that: raw material is fresh or dry oil tea or Camellia olelfera Abel [C.oleosa (Lour.) Rehd., 1% 2% aqueous alkalis soak 15 minutes, surface moisture is drained after rinsed clean, breaking into oleo stock, addition is 40% 60%.

7. a kind of nectar source according to claim 1 bacterium fermentation preparation oil tea or the method for Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage, is characterized in that: water refers to pure water or mineral water, and addition is 50% 10%. .

8. a kind of nectar source according to claim 1 bacterium fermentation preparation oil tea or the method for Camellia olelfera Abel [C.oleosa (Lour.) Rehd. Mel enzyme beverage, is characterized in that: will not be bacterial contamination in sweat, and after fermentation, tea leaf quality promotes, and tea beverage mouthfeel improves by front oil tea of relatively fermenting.