CN105660418A - Full-automatic tissue culture room capable of making full use of space - Google Patents
Full-automatic tissue culture room capable of making full use of space Download PDFInfo
- Publication number
- CN105660418A CN105660418A CN201610214246.8A CN201610214246A CN105660418A CN 105660418 A CN105660418 A CN 105660418A CN 201610214246 A CN201610214246 A CN 201610214246A CN 105660418 A CN105660418 A CN 105660418A
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- Prior art keywords
- group
- chamber
- training
- interior chamber
- space
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention provides a full-automatic tissue culture room capable of making full use of the space, and belongs to the field of plant tissue culture equipment. The tissue culture room comprises an outer room, an inner room and a control system, wherein the outer room is irradiated by ultraviolet rays for a long time for sterilization; technical personnel can be buffered in the outer room at first when going into and out the tissue culture room and then can go into the inner room after wearing germfree clothes, so that the possibility that external substances unfavorable for a tissue culture environment are brought into the tissue culture room is reduced to a certain extent; furthermore, due to position replacement between every two layers of a tissue culture rack, the technical personnel can easily manage tissue culture seedlings at high places, and the space can be saved by fully using the space height; and in the tissue culture room, the control system is used for performing uniform control on the tissue culture room to realize real-time, accurate and intelligent control. The full-automatic tissue culture room capable of making full use of the space, which is provided by the invention, has the advantages of automation, low environment germ density and space saving.
Description
Technical field
The invention belongs to plant tissue culture apparatus field, relate in particular to a kind of full-automatic group of training chamber that makes full use of space.
Background technology
Tissue culture technique is under aseptic condition, work organ, tissue or cell to be placed in culture medium, and be placed in adapt circumstance, carry out cell, tissue or individuality that Continuous Cultivation forms, it mainly utilizes cell totipotency, be that each cell has a set of complete inhereditary material, induce the plant tissue having broken up to carry out dedifferentiation by the specific combination of the various hormones in culture medium, thereby induce into callus, more further induce the individuality that makes new advances. This technology has been widely used in agricultural and biological, medical research, for huge contribution has been made in micropropagation of plants, plant species protection and correlative study.
But contain a large amount of nutriments owing to organizing to cultivate in the culture medium using, not only be applicable to plant cell and tissue growth, also be applicable to various bacteriums and conk, the growth meeting of bacterium consumes the nutriment in culture medium in a large number, and the growth of plant cell is impacted simultaneously. In addition because Calli Differentiation degree is low, defensive ability/resistance ability to bacterium and fungi is poor, as easy as rolling off a logly be subject to the material impact that bacterium and fungi discharge, or poisoned, or produce variation, tissue is cultivated to industry and cause great impact, so clean aseptic environment is cultivated of crucial importance to tissue.
Group training chamber is the important means of control group training environment, but current group training chamber mostly is a common room, unavoidably can be by extraneous bacterium when technical staff passes in and out, it is indoor that fungies etc. are unfavorable for that the material of group training environment takes group training to, the effect that impact tissue is cultivated, in addition because group training seedling grows in tissue culture bottle, can neatly discharge, can make full use of spatial altitude plantation, can influence technique personnel observe and daily management mission but group training frame is too high, rely on staff to go monitoring and control group to train indoor environmental change simultaneously and often can not make adjustment timely, in the time of regulation and control, have certain hysteresis quality, also likely because of artificial carelessness, Plant Tissue Breeding effect is impacted. .
Summary of the invention
There are the problems referred to above based on prior art, the invention provides a kind of full-automatic group of training chamber that makes full use of space, it comprises mistress, interior chamber and control system, mistress comprises changing rooms, disinfection system, interior chamber comprises group training frame, detection system, temperature control system, group training light-source system, illuminator, and control system comprises PLC control system and host computer; Mistress uses ultraviolet radiation sterilization for a long time, when passing in and out, technical staff can first in mistress, cushion, change aseptic clothes and enter again interior chamber, reduce to a certain extent the extraneous material that is unfavorable for group training environment and be brought to the indoor possibility of group training, train the position replacement of the every interlayer of frame by group simultaneously, technical staff can manage the group training seedling of eminence like a cork, can make full use of spatial altitude and save space, group training chamber utilizes control system group training chamber to be carried out to unified control can be accomplished in real time, the control of accurate and automation. Provided by the inventionly a kind ofly make full use of full-automatic group of space training chamber and there is the low and space-saving advantage of automation, environmental bacteria density.
A full-automatic group of training chamber that makes full use of space, it comprises mistress, interior chamber and control system;
Mistress comprises changing rooms, disinfection system, and interior chamber comprises group training frame, detection system, temperature control system, group training light-source system, illuminator, and control system comprises PLC control system and host computer;
Group training frame is neatly placed in interior chamber, between each group of training frame, leaves passageway, every layer, group training frame can be by gear driven along rail moving and switch; Detection system comprises temperature sensor and intensity of illumination sensor, is hung on the wall at four sides, interior chamber; Temperature control system comprises air-conditioning, puts in interior chamber; Group training light-source system comprises white light light pipe, is arranged on the top of every one deck of each group of training frame; Illuminator is arranged on group training top, chamber;
Changing rooms is positioned at outer indoor, comprises public gown of a doctor and cap after clothes hanger, sterilizing in changing rooms; Disinfection system comprises ultraviolet light pipe, is arranged on mistress top;
Interior chamber is placed in mistress mutually, can enter interior chamber from mistress by the doorway of interior chamber, and control system is positioned at outer indoor, is connected with other system, controls and coordinates each system operation.
Wherein, on mistress's doorframe, there is supply air system, towards blowing outdoors, supply air system on doorframe outwards sprays filtrated air and can stop to a certain extent the harmful substances such as dust floating in outside air, bacterium and fungi to enter into outer indoor, also can blow away the harmful substances such as the personnel's of entering dust with it, ensure that mistress's environment is more clean than external environment.
Wherein, on the doorframe of interior chamber, there is supply air system, towards blowing outdoors, supply air system on doorframe outwards sprays filtrated air and can stop to a certain extent the harmful substances such as dust floating in mistress's air, bacterium and fungi to enter in interior chamber, also can blow away the harmful substances such as the personnel's of entering dust with it, in ensureing, the environment of chamber is than mistress clean environment.
Wherein, the doorway on interior chamber and mistress is built up with dislocation form, can stop outside air to be directly communicated with interior chamber air, and entering into from the external world must be through mistress's buffering among interior chamber.
Wherein, on the wall that interior chamber and mistress are separated by, have can ultraviolet ray filtering windowpane, can observe from the inside chamber of mistress by windowpane, windowpane makes technical staff can observe from mistress the situation of interior chamber, at any time spy's condition of oestrusing is made to strain, filter glass can stop mistress's ultraviolet ray to be irradiated in interior chamber simultaneously, causes plant tissue variation.
Wherein, the group training light-source system in interior chamber is by timing power-on switch connection group training chamber power supply, and in automatically controlling, the group training light source of chamber is opened, and builds the alternation of light and darkness phenomenon in natural environment.
Wherein, interior chamber also has disinfection system, is arranged on the top of interior chamber, and the disinfection system that can open top, interior chamber after a collection of Plant Tissue Breeding finishes carries out sterilization treatment thoroughly to whole group of training chamber.
Wherein, the group training frame in interior chamber is connected with motor, can drive group train the place-exchange of carrying out between the different layers on frame by motor, makes the group training seedling of putting in eminence can move to lower, facilitates technical staff's daily management.
Wherein, the host computer in control system is first connected with PLC control system, and PLC control system is connected with the indoor other system of group training again.
The invention has the beneficial effects as follows: using common tissue culture room as interior chamber, adding one deck mistress in its outside cushions, avoid group training chamber to be directly communicated with extraneous, reduce extraneous be suspended in the technical staff that dust in air, bacterium and fungi etc. enter or passed in and out and be brought in group training chamber, affect group training environment; Technical staff can more change one's clothes in mistress, further reduces the possibility of being polluted of pollution group training chamber; Mistress opens for a long time disinfection system in the time not using, and makes mistress always in state aseptic or that bacterial density is extremely low, can play a good cushioning effect for group training chamber, the possibility that minimizing group training chamber is polluted; Can switch between every layer, utilization group training frame and the group training seedling of eminence is moved to lower, facilitate daily management, make full use of spatial altitude and the area of saving group training chamber, reach space-saving object; Use control system to carry out unified coordination and control to group training chamber, can accomplish the control of real-time, accurate and automation.
Brief description of the drawings
Fig. 1, a kind of structural representation of the full-automatic group of training chamber that makes full use of space.
Fig. 2, a kind of side structure schematic diagram of group training frame of the full-automatic group of training chamber that makes full use of space.
Fig. 3, a kind ofly makes full use of in full-automatic group of space training chamber control system and other system this links schematic diagram.
Wherein 1-mistress, chamber in 2-, 3-control system, 11-changing rooms, 12-disinfection system, 21-group training frame, 22-detection system, 23-temperature control system, 24-group training light-source system, 25-illuminator, 211-gear, 212-track, 31-PLC control system, 32-host computer.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the invention will be further described.
A kind of full-automatic group of training chamber that makes full use of space as shown in Figure 1, it comprises mistress (1), interior chamber (2) and control system (3);
Mistress (1) comprises changing rooms (11), disinfection system (12), interior chamber (2) comprises group training frame (21), detection system (22), temperature control system (23), group training light-source system (24), illuminator (25), and control system (3) comprises PLC control system (31) and host computer (32);
It is inner that group training frame (21) is neatly placed in interior chamber (2), between each group of training frame (21), leaves passageway, and every layer, group training frame can pass through gear (211) and drive along track (212) and move and switch; Detection system (22) comprises temperature sensor and intensity of illumination sensor, is hung on the wall at four sides, interior chamber (2); Temperature control system (23) comprises air-conditioning, puts in interior chamber (2) inner; Group training light-source system (24) comprises white light light pipe, is arranged on the top of every one deck of each group of training frame (21); Illuminator (25) is arranged on group training top, chamber;
Changing rooms (11) is positioned at mistress (1), comprises public gown of a doctor and the cap after clothes hanger, sterilizing in changing rooms (11); Disinfection system (12) comprises ultraviolet light pipe, is arranged on mistress (1) top;
Interior chamber (2) is placed in mistress (1) mutually, can enter interior chamber (2) from mistress (1) by the doorway of interior chamber (2), and control system (3) is positioned at mistress (1), is connected with other system, controls and coordinates each system operation.
As preferred embodiment, on mistress's (1) doorframe, there is supply air system, towards blowing outdoors.
As preferred embodiment, on the doorframe of interior chamber (2), there is supply air system, towards blowing outdoors.
As preferred embodiment, the doorway on interior chamber (2) and mistress (1) is built up with dislocation form.
As preferred embodiment, on the wall that interior chamber (2) and mistress (1) are separated by, have can ultraviolet ray filtering windowpane, can inwardly observe chamber (2) from mistress (1) by windowpane.
As preferred embodiment, interior chamber (2) inner group training light-source system (24) is by timing power-on switch connection group training chamber power supply.
As preferred embodiment, interior chamber (2) also have disinfection system (12), are arranged on the top of interior chamber (2).
As preferred embodiment, the group training frame in interior chamber is connected with motor, can drive group train the place-exchange of carrying out between the different layers on frame (21) by motor.
As preferred embodiment, the host computer (32) in control system (3) is first connected with PLC control system (31), and PLC control system (31) is connected with the indoor other system of group training again.
As preferred embodiment, technical staff can set group training chamber according to the condition of tissue culture of different plants in control system (3), the air-conditioning of serviceability temperature control system (23) arranges the indoor temperature of group training, makes the indoor temperature of group training remain on the optimum temperature of plant tissue growth.
As preferred embodiment, technical staff can set group training chamber according to the condition of tissue culture of different plants in control system (3), use timing energization switch that opening and light of group training light source is set, group training lab simulation go out sooner or later substitute natural phenomena.
As preferred embodiment, technical staff can set intensity of illumination in control system (3) according to the condition of tissue culture of different plants, by opening the light pipe controlled light intensity in the group training light source of varying number, to reach the intensity of illumination of applicable plant tissue growth.
As preferred embodiment, the complete plant tissue culture bottle of inoculation is positioned on group training frame (21), set corresponding temperature, intensity of illumination and light application time according to the required condition of Plant Tissue Breeding, group training chamber is according to setup control group training environment, and technical staff enters the upgrowth situation of observation of plant tissue in group training chamber every day.
As preferred embodiment, place while inoculating complete tissue culture bottle and can first occupy group training rack-layer below, then use motor by upper and lower group training rack-layer switch, then place tissue culture bottle; Train seedling in the group that can first handle equally low layer to organizing when training seedling carries out daily management, then exchange remaining group of training seedling of position continuation processing of levels group training rack-layer by motor.
Technical staff first closes the disinfection system (12) in the group outdoor chamber of training (1) need to enter group training chamber time, then enter the group outdoor chamber of training (1), constantly outwards air-supply of supply air system in the time entering on the doorframe of group training outdoor chamber (1), technical staff's material such as dust, bacterium is with it blown away and stops the suspension in outside air to enter, enter gown of a doctor, disposable slippers and the cap of changing sterilizing after mistress (1) at changing rooms (11).
After changing one's clothes, enter again the group indoor chamber of training (2), in the time entering the group indoor chamber of training (2), supply air system on the doorframe of interior chamber (2) is outwards air-supply constantly, stop the airborne suspension of mistress (1) to enter interior chamber (2) with it technical staff, technical staff enters the upgrowth situation of the rear observation of plant tissue in interior chamber (2), after observation, leave interior chamber (2), change own clothes mistress (1), then leave mistress (1), walk out the disinfection system (12) that reopens mistress (1) after mistress (1).
Use a kind of full-automatic group of training chamber that makes full use of space provided by the invention, can greatly reduce the indoor airborne bacterial density of group training, greatly reduce the possibility that Plant Tissue Breeding is subject to germ contamination, reduce significantly pollution rate, take full advantage of the eminence space of group training chamber simultaneously, save the area of group training chamber, can realize full-automatic, accurate and real-time control, improved the quality that tissue is cultivated.
The above embodiment has only expressed one embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention. It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention. Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (8)
1. a full-automatic group of training chamber that makes full use of space, is characterized in that, it comprises mistress, interior chamber and control system;
Mistress comprises changing rooms, disinfection system, and interior chamber comprises group training frame, detection system, temperature control system, group training light-source system, illuminator, and control system comprises PLC control system and host computer;
Group training frame is neatly placed in interior chamber, between each group of training frame, leaves passageway, every layer, group training frame can be by gear driven along rail moving and switch; Detection system comprises temperature sensor, is hung on the wall at four sides, interior chamber; Temperature control system comprises air-conditioning, puts in interior chamber; Group training light-source system comprises white light light pipe, is arranged on the top of every one deck of each group of training frame; Illuminator is arranged on group training top, chamber;
Changing rooms is positioned at outer indoor, comprises public gown of a doctor and cap after clothes hanger, sterilizing in changing rooms; Disinfection system comprises ultraviolet light pipe, is arranged on mistress top;
Interior chamber is placed in mistress mutually, can enter interior chamber from mistress by the doorway of interior chamber, and control system is positioned at outer indoor, is connected with other system, controls and coordinates each system operation.
2. according to a kind of full-automatic group of training chamber that makes full use of space of claim 1, it is characterized in that thering is supply air system on the doorframe of described mistress and Nei chamber, towards blowing outdoors.
3. according to a kind of full-automatic group of training chamber that makes full use of space of claim 1, it is characterized in that, the doorway on described interior chamber and mistress is built up with dislocation form.
4. the full-automatic group of training chamber that makes full use of space according to claim 1 a kind of, is characterized in that, on the wall that described interior chamber and mistress are separated by, have can ultraviolet ray filtering windowpane, can observe from the inside chamber of mistress by windowpane.
5. according to a kind of full-automatic group of training chamber that makes full use of space of claim 1, it is characterized in that, the group training light-source system in described interior chamber is by timing power-on switch connection group training chamber power supply.
6. according to a kind of full-automatic group of training chamber that makes full use of space of claim 1, it is characterized in that, described interior chamber also has disinfection system, is arranged on the top of interior chamber.
7. according to a kind of full-automatic group of training chamber that makes full use of space of claim 1, it is characterized in that, the group training frame in described interior chamber is connected with motor, can drive group train the place-exchange of carrying out between the different layers on frame by motor.
8. according to a kind of full-automatic group of training chamber that makes full use of space of claim 1, it is characterized in that, the host computer in described control system is first connected with PLC control system, and PLC control system is connected with the indoor other system of group training again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610214246.8A CN105660418A (en) | 2016-04-08 | 2016-04-08 | Full-automatic tissue culture room capable of making full use of space |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610214246.8A CN105660418A (en) | 2016-04-08 | 2016-04-08 | Full-automatic tissue culture room capable of making full use of space |
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| CN105660418A true CN105660418A (en) | 2016-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610214246.8A Pending CN105660418A (en) | 2016-04-08 | 2016-04-08 | Full-automatic tissue culture room capable of making full use of space |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203732970U (en) * | 2014-03-03 | 2014-07-23 | 上海邦伯现代农业技术有限公司 | Tissue culture room intelligent monitor |
| CN204377626U (en) * | 2014-11-06 | 2015-06-10 | 安徽省农业科学院园艺研究所 | Energy-efficient group of training room |
-
2016
- 2016-04-08 CN CN201610214246.8A patent/CN105660418A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203732970U (en) * | 2014-03-03 | 2014-07-23 | 上海邦伯现代农业技术有限公司 | Tissue culture room intelligent monitor |
| CN204377626U (en) * | 2014-11-06 | 2015-06-10 | 安徽省农业科学院园艺研究所 | Energy-efficient group of training room |
Non-Patent Citations (3)
| Title |
|---|
| 刘建福等: "《细胞工程》", 30 June 2014, 华中科技大学出版社 * |
| 郑春明等: "《植物组织培养技术》", 31 July 2011, 浙江大学出版社 * |
| 陈世昌: "《植物组织培养》", 30 April 2011, 高等教育出版社 * |
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Application publication date: 20160615 |