CN105648020A - Low-carbon carotenoid production method - Google Patents

Abstract

The invention discloses a low-carbon carotenoid production method. Trimmings of canned yellow peaches are taken as a main raw material and subjected to microwave color fixing, freezing, breaking and biological enzymolysis, juice with higher carotenoid content is obtained, a fermentation medium is obtained after component adjustment, Rhodotorula benthica with high carotenoid yield is inoculated to the fermentation medium for graded inoculation and temperature-shift fermentation, the Rhodotorula benthica is enabled to be proliferated to the greatest extent, fermentation broth is subjected to ultrahigh-pressure homogenization, vacuum centrifugation, demulsification and separation, an emulsion is obtained, and the finished product carotenoid is obtained after extraction, separation and purification of the emulsion, wherein the cell biomass of the fermentation broth is 38-52 g/L, and the yield of the carotenoid is 47-61 mg/L. The production method is simple, short in cycle, high in efficiency, energy-saving and environment-friendly, adopts low-temperature and bio-processing technologies in the whole process, reduces the production cost, eliminates hidden danger of environmental pollution, lays a firm foundation for sustainable development in the technical field of deep processing of the canned yellow peaches and has positive economic and social development significance.

Description

A kind of low-carbon (LC) produces the method for carotenoid

Technical field

The present invention relates to the preparation of carotenoid, be specifically related to a kind of method that low-carbon (LC) produces carotenoid.

Background technology

Carotenoid is the general name of one group of fat-soluble pigment with non-oxidizability, it is as the class important biomolecule active substance in humans and animals body, there is the important function such as enhancing human body immunity power, radioprotective, antitumor and treatment photosensitive diseases, have a wide range of applications in industries such as food, cosmetics, medicine and feedstuffs. At present, carotenoid is regarded as A class nutrition pigment by international organizations such as FAO, the European Community and WHO, and in recent years, the industrialization development around carotenoid also gradually forms.

The industrialized preparing process of carotenoid can adopt chemical synthesis or plant extraction method. Chemosynthesis carotenoid not only technical sophistication, and it can affect the taste of food as food additive use, reduces the ratio of absorption of human body, there is also certain side effect simultaneously. Therefore, the psychology advocating pollution-free food along with people strengthens day by day, and research direction is turned to the development and utilization of natural pigment by increasing scholar in recent years. Conventional preparation techniques owing to extracting carotenoid from plant has higher cost and Operating Complexity, and therefore, the industrialized production of carotenoid receives huge restriction. Utilizing fermentable to produce carotenoid, not only with low cost, technique is simple, and ensure that high-quality and the stable yields of product, and therefore, utilizing micro-organisms carotenoid is the main path that living resources type carotenoid is originated.

At present, the microbial strains that can produce carotenoid reported is mainly trispore Bruce mould and Rhodothece glutinis, and the former is already at pilot scale and commercial application stage, and the latter Ze Shang is in the laboratory lab scale stage. Although trispore Bruce mould pigment production is high, but culture process is complicated, and the production cycle is long, it is simple that Rhodothece glutinis production carotenoid then has nutritional requirement, cultivation cycle is short, and thalline contains the advantage that rich in protein, aminoacid and vitamin etc. can comprehensively utilize.

Chinese patent CN105039484A discloses one and utilizes ocean rhodotorula High Yield of Carotenoid and copper fermentation culture method, including as follows: described ocean rhodotorula WYN1 fermentation culture in cupric culture medium efficiently produces carotenoid, fermentation medium consists of glucose 1%, enzymolysis Semen Maydis powder 1-3%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 150-200mg/L, sodium chloride 20%, all the other are water;Initial pH is 8, inoculates 3-8%, and shaking speed is 50-80r/min, 24-28 DEG C and cultivates and adjust temperature after 10-15 hour and be 16-20 DEG C and carry out low temperature static gas wave refrigerator, and stopping stirring, pH is adjusted to 3-5, keeps 2-4 hour; Stairstepping is warmed up to 24-30 DEG C afterwards, and pH is adjusted to 4.6-5.5, and the addition according to 2.5% adds peptone, and the amount according to 2% adds yeast powder; Shaking speed is 50-100r/min, continues fermentation 10-15h.

Chinese patent CN104130952A discloses a strain rhodotorula mucilaginosa and the application in fermenting and producing carotenoid and oils and fats thereof. Technical scheme main points are: a Rhodotorula mucilaginose strain Rhodotorulamucilaginosa, are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, CGMCCNo.8926. Rhodotorula mucilaginosa provided by the invention can be used for fermenting and producing carotenoid and oils and fats. The present invention utilizes Rhodothece glutinis produce carotenoid and produce oils and fats simultaneously, and carry out taking turns ARTP (atmospheric pressure at room plasma) mutation to producing bacterial strain more, obtain the mutagenic strain that yield is higher, the industrialized production of carotenoid and oils and fats is provided with good application prospect and economic benefit.

Some antibacterials also can produce carotenoid: Chinese patent CN104805168A discloses a kind of method utilizing photosynthetic bacteria micro-aerobe fermentation quickly to produce carotenoid: is seeded in MMS culture medium by the photosynthetic bacteria (Rhodobactersphaeroides) of activation, the aerobic OD600 of being cultured to is 0.6-0.8, then condition of culture extremely micro-oxygen is regulated, inducing carotenoid rapid, high volume synthesizes, then extract, purification, the time utilizing the inventive method fermenting and producing carotenoid is short, only need 36 hours, and thalline yield is up to 6g/L, the yield of carotenoid and productivity are 8.287mg/L and 1911 �� g/g respectively, there is the good ability removing DPPH free radical, IC50 is about 8 �� g/mL.

Chinese patent CN102732049B discloses a kind of method that carotenoid is prepared in extraction from microbial cells. The method is after microbial cells fermentation ends, and fermentation liquid separates with thalline and obtains wet thallus, imports in cavity charge containers again through logistics pipeline and carries out high steam explosion breaking cellular wall; Wet thallus dehydration after cell breakage, obtains the filter cake that bacterial chip is formed; Every kilogram of filter cake adds organic solvent 10��25 liters, 30��55 DEG C of stirring and leaching 20��50 minutes; Filter, obtain the organic solvent extracting solution containing carotenoid oils and fats; Extracting solution is vacuum concentration under 40��60 DEG C of conditions, reclaims organic solvent, obtains carotenoid oils and fats, and single-trial extraction yield reaches more than 90%. This method saves the dehydrate in traditional handicraft and thalline pulverizing process, and technological process is short, single-trial extraction yield high and the used time is short, organic solvent consumption reduces, and energy consumption and other costs significantly reduce. Whole process is simple, quality controllable, is suitable for industrialized production application.

Patent disclosed above is which kind of microorganism whether, and its fermentation is prepared the raw material of carotenoid and is conventional medium food ingredient, relatively costly, and class Hu Luosu acquisition rate yield and productivity all relatively low, be not suitable for current low-carbon (LC) produce environmental requirement.

Chinese patent CN102286594A discloses a kind of method utilizing high microsteping agricultural byproducts fermenting and producing carotenoid, it is that high microsteping agricultural byproducts raw material pulverizing is processed, profit water steaming after mixing homogeneously with adjuvant i.e. 0��6.0% ammonium sulfate and/or 0��3.0% potassium dihydrogen phosphate and/or 0��1.0% calcium chloride and/or 0��1.0% magnesium sulfate;Steaming cools down 20��40 DEG C after completing, and meets sturdy vein born of the same parents bacterium, illumination cultivation 2��10d at 20��40 DEG C; By cultured culture medium or the collection dried acid system breaking cellular wall of spore, then with acetone or ethyl acetate lixiviate carotenoid; Carried pigment is easily separated, makes carotenoid finished product after purification. This method has process easy operation control, and production cost is low, and fermentation period is short, carotenoid output advantages of higher. Patent fermentation period disclosed above is longer, and production cost is high, and the yield of carotenoid and productivity are still undesirable.

The nutrition of yellow peach is very abundant, and its Major Nutrient composition has: cellulose, carotene, lycoxanthin, red pigment and the various trace elements that abundant vitamin C and substantial amounts of needed by human body are wanted. If selenium, zinc equal size are obviously higher than other common Fructus Persicae, possibly together with the composition such as malic acid, citric acid. Often eat yellow peach and the heat maintaining brain function can not only be provided, the lipid metabolism in health can also be regulated, eat two every day and may only play relieving constipation, blood sugar lowering, blood fat, free radical resisting, dispel the effects such as black speck, slow down aging, raising immunologic function, also can promote appetite, can be rated as the Fructus Persicae of health fruit, health preserving. Easily tired people, is well suited for often eating yellow peach the people of contaminated environment work, the people having a passion for one's pipe, the people that is engaged in strenuous exercise and the people of highly intensive labour, Long-term taking medicine.

Due to yellow peach pole not for shelf-stable, currently mainly based on canned food deep processing, the leftover bits and pieces that canned food produces, such as the peel reamed, the pit cut out and cull fruit etc., account for about the 30% of raw material weight, major part rots, or is thrown away, macerates into fertilizer, and what have is piled up in outside factory and causes public hazards, contaminated environment and wastage of material are very big, add production cost simultaneously. In general, every hectogram yellow peach sarcocarp containing carotenoid between 1-10 milligram, carotenoid storage rate average out to about 40% after can processing, its amount containing carotenoid number be also one of the index weighing Yellow-peach can quality. Except improving technique reserved category carotene as much as possible, adding carotene is also the important means improving yellow peach quality. Yellow peach peel is one of leftover bits and pieces of Yellow-peach can, and every hectogram peel is containing reducing sugar 2.4 grams, total protein 0.037 gram, and carotene 0.0085 gram, possibly together with multiple abundant vitamin. There is good comprehensive recycle value again.

At present, yet there are no pertinent literature or the report of preparing carotenoid with Yellow-peach can leftover bits and pieces for fermenting raw materials.

Summary of the invention

Solved by the invention technical problem is that overcomes existing fermentation to prepare the defect of class Hu Luosu, with Yellow-peach can leftover bits and pieces for primary raw material, through microwave color fixing, freezing crushing, biological enzymolysis obtains the fruit juice that carotenoid content is higher, fermentation medium is obtained after adjusting component, the ocean rhodotorula inoculating high yield class Hu Luosu wherein carries out gradation inoculation, temperature-variable fermentation, ocean rhodotorula is made to maximize propagation, fermentation liquor extra high pressure homogenize, traditional vacuum, emulsion is obtained after break milk separation, emulsion is extracted, separate, finished product carotenoid is obtained after purification.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of low-carbon (LC) produces the method for carotenoid, comprises the steps:

1) juice preparation: first Yellow-peach can leftover bits and pieces is put in microwave dryer in power 3-5kW, thickness of feed layer 2-4cm, 105-115 DEG C, dry 2-4min, then 10-15min in the sericin peptide taken solution that mass percent is 10-14% it is immersed in, pulverize immediately after-18--22 DEG C of freezing 20-40min, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.3-0.5mm, it is subsequently added into the water of ground product quality 1-3 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 �� s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz,Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction; Add the mixed enzyme of extracting solution quality 3-5%, in 40-50 DEG C of enzymolysis 30-50min, filter, obtain fruit juice;

Further, described Yellow-peach can leftover bits and pieces is: any one or two kinds in yellow peach peels, inferior yellow peach sarcocarp mix with any mass ratio;

Further, described mixed enzyme is cellulase, protease, pectase, tannase 2-4:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio;

2) prepared by fermentation medium: to step 1) fruit juice prepared adds the protective agent of its quality 5-9%, the sucrose of 1-3%, the ammonium sulfate of 0.5-1.5%, the potassium dihydrogen phosphate of 2-4%, the calcium chloride of 0.5-1.5% and the magnesium sulfate of 0.5-1.5% obtain fermentation medium;

Further, described protective agent with containing antifreeze protein, significantly improve Rhodothece glutinis and resist the plant of hot and cold irritability, disease resistance and immunity for raw material, prepared through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and compound enzyme enzymolysis, the cold-and-heat resistent stress ability of Rhodothece glutinis in sweat can be effectively improved, improve its multiplication capacity and Viable detection, activity extra dry red wine yeast powder can be improved at freezing dry process Viable detection simultaneously;

Preferably, described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, the Radix Astragali, Radix Codonopsis, Herba Epimedii, Herba Houttuyniae is respectively washed, drain, 8-10:5-7:4-6:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 3-8h that pH value is 3.8-4.5 of mixed material quality 0.1-1 times, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.5-3mm, it is subsequently added into the water of ground product quality 10-20 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 �� s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz, then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction, add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 3-5:2-4:1-3:1-3:1-2 Homogeneous phase mixing in mass ratio;

Extract it is highly preferred that described microwave irradiation and extraction is batch (-type), i.e. microwave exposure 10s, interval 20s;

3) fermentation, homogenizing breaking cellular wall: to step 2) the active extra dry red wine yeast powder that adds its quality 0.2-0.4% in fermentation medium controls temperature 30-34 DEG C, speed of agitator 50-100r/min, ferment at constant temperature 12-15h, then with the ramp of 0.8-1.0 DEG C/min to 40-42 DEG C, add the active extra dry red wine yeast powder constant temperature static fermentation 3-5h of fermentation medium quality 0.1-0.3%, finally it is cooled to 28-32 DEG C with the speed of 0.6-0.8 DEG C/min, continue static fermentation 15-20h, fermentation liquid is successively through 40 orders, 80 order duplex strainers filter, filtrate through superhigh-voltage homogenizing machine in 2-4 DEG C, 145-155MPa homogenizing breaking cellular wall obtains homogenizing fluid,

Further, described activity extra dry red wine yeast powder is prepared with ocean rhodotorula CCTCCNo:M2014592 disclosed in Chinese patent CN105039484A according to a conventional method for the bacterium that sets out;

Preferably, the protective agent used in activity extra dry red wine yeast powder preparation process is step 2) the middle protective agent prepared;

4) breakdown of emulsion, separation and Extraction: homogenizing fluid is put 6000-8000r/min traditional vacuum 10-15min in vacuum centrifuge, separate from top to bottom and obtain free oil, emulsion, hydrolyzed solution and solid residue, the emulsion obtained is controlled temperature and is 30-50 DEG C, add the biological demulsifying agent breakdown of emulsion 40-60min of emulsion quality 0.01-0.03%, free oil and emulsion is obtained in 2000-3000r/min traditional vacuum 8-12min again after breakdown of emulsion, the free oil merging gained separately uses it for anything else, and gained emulsion is extracted, separate, obtain finished product carotenoid after purification.

Further, described traditional vacuum condition is temperature 12-18 DEG C, vacuum-0.01--0.03MPa;

Further, described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall are in conjunction with the one or more combination in class biological demulsifying agent;

Preferably, the quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=3-5:2-4:1-3;

Preferably, described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl polyglucoside;

It is highly preferred that the quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=4-6:1-2;

The detection method of cellular biomass disclosed in Chinese patent CN105039484A and the assay method of carotenoid to the cellular biomass in the fermentation liquid of above-mentioned preparation and in emulsion the carrying out of carotenoid detect: cellular biomass is 38-52g/L; Carotenoid output is 47-61mg/L.

Beneficial effect:

The present invention is with Yellow-peach can leftover bits and pieces for raw material, while making leftover bits and pieces explosion puffing drying, enzyme denaturing, fixation it is mainly initially with microwave drying, remain the natural colored of leftover bits and pieces and the content of carotenoid to greatest extent, leftover bits and pieces natural colored after microwave color fixing is highly stable in the follow-up course of processing, will not variable color, fade, carotenoid content quite stable, loses extremely low; Aqueous solution containing sericin peptide taken soaks rehydration, the loss of leftover bits and pieces active substance that is chilled and that cause can be reduced to greatest extent, improve the extraction ratio of leftover bits and pieces effective ingredient, also establish solid foundation for follow-up alternating temperature activity extra dry red wine culture propagation simultaneously; The extract at low temperature technology such as high-pressure pulse electric, microwave, ultrasonic, biological enzymolysis is organically combined, bioactive ingredients content and the nutritional labeling such as class Hu Luosu of heel can be retained to greatest extent, effectively prevent course of processing living contaminants; The composite protective agent of science in the fermentation medium, is remarkably improved ocean rhodotorula and resists hot and cold irritability, disease resistance and immunity so that it is fast activating, rejuvenation, it is achieved Rhodothece glutinis quality and quantity maximizes, and then realizes the high yield of class Hu Luosu; With the ocean rhodotorula of High Yield of Carotenoid for leaven, temperature-variable fermentation and gradation vaccination ways is adopted can effectively to shorten lag phase and period of decline time, substantially prolongs exponential phase and stable phase, obtain the maximum accumulation of ocean rhodotorula propagation and carotenoid to greatest extent, improve its multiplication capacity and Viable detection; By extra high pressure homogenize breaking cellular wall ocean rhodotorula cell, sporoderm-broken rate is high, and class Hu Luosu burst size is big; Traditional vacuum and biological demulsifying will be adopted to organically combine, significantly improve the extraction ratio of carotenoid, improve raw material availability, reduce extraction cost.The kind of by-product after microbial grease etc. extracts, yield and added value is added while improving product quality and extraction ratio; reduce further the extraction cost of carotenoid; stop the discharge of " three wastes ", protected environment, be truly realized low-carbon (LC) and produce.

Cellular biomass in the fermentation liquid that the present invention is prepared by the detection method of cellular biomass disclosed in Chinese patent CN105039484A and the assay method of carotenoid and in emulsion the carrying out of carotenoid detect: cellular biomass is 38-52g/L; Carotenoid output is 47-61mg/L.

The preparation method of the present invention is simple, cycle is short, efficiency is high, save the energy, environmentally friendly, omnidistance employing low temperature, biological processing technology, effectively prevent the loss of the heat-sensitive nutrition such as carotenoid, vitamin in leftover bits and pieces, improve the extraction ratio of carotenoid and biological activity and nutrition content, improve yield and the quality of product, reduce production cost, present invention also offers a kind of method that separation obtains microbial grease simultaneously, there is good practicality and advance.

It should be noted that and the solution have the advantages that each synergistic summation of step technique feature, there is between each step certain inherent dependency, not the simple superposition of single technical characteristic effect.

It can further be stated that the present invention prepares class Hu Luosu with Yellow-peach can leftover bits and pieces for raw material, deep processing for yellow peach opens a new shortcut, it is effectively reduced the burden of processing enterprise, significantly reduce production cost, add value-added content of product, eliminating environmental pollution hidden danger, solid foundation has been established in the sustainable development for the deep process technology field of yellow peach, has positive economy and social development meaning.

Detailed description of the invention

The present invention is described below by specific embodiment. Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art. It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow. To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.

Embodiment 1

A kind of method utilizing yellow peach peels fermenting and producing carotenoid, comprises the steps:

1) juice preparation: first yellow peach peels is put in microwave dryer in power 4kW, thickness of feed layer 3cm, 110 DEG C, dry 3min, then 12min in the sericin peptide taken solution that mass percent is 12% it is immersed in, pulverize immediately after-20 DEG C of freezing 30min, freezing thickness of feed layer 4cm, ground product particle diameter 0.4mm, it is subsequently added into the water of ground product quality 2 times, it is 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 �� s, carries out high voltage pulse electric field processing 25min when pulse frequency 250Hz; Then carry out microwave irradiation and extraction 18min when power 200W in room temperature, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction; Add the mixed enzyme of extracting solution quality 4%, in 45 DEG C of enzymolysis 40min, filter, obtain fruit juice;

Described mixed enzyme is cellulase, protease, pectase, tannase 3:3:2:2 Homogeneous phase mixing in mass ratio;

2) prepared by fermentation medium: to step 1) fruit juice prepared adds the protective agent of its quality 7%, 2% sucrose, 1% ammonium sulfate, the potassium dihydrogen phosphate of 3%, the calcium chloride of 1% and 1% magnesium sulfate obtain fermentation medium;

Described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, the Radix Astragali, Radix Codonopsis, Herba Epimedii, Herba Houttuyniae is respectively washed, drain, 9:6:5:4:3:2 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 5h that pH value is 4.2 of mixed material quality 0.5 times, pulverize immediately after-20 DEG C of freezing 1.5h, freezing thickness of feed layer 4cm, ground product particle diameter 2mm, it is subsequently added into the water of ground product quality 15 times, it is 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 �� s, high voltage pulse electric field processing 25min is carried out when pulse frequency 250Hz, then microwave irradiation and extraction 18min is carried out in room temperature when power 200W, wherein, microwave exposure 10s, interval 20s, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction, add the compound enzyme of extracting solution quality 1.5%, in 50 DEG C of enzymolysis 40min, enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.2mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 4:3:2:2:1.5 Homogeneous phase mixing in mass ratio;

3) fermentation, homogenizing breaking cellular wall: to step 2) the active extra dry red wine yeast powder that adds its quality 0.3% in fermentation medium controls temperature 32 DEG C, speed of agitator 80r/min, ferment at constant temperature 13h, then with the ramp of 0.9 DEG C/min to 41 DEG C, add the active extra dry red wine yeast powder constant temperature static fermentation 4h of fermentation medium quality 0.2%, finally it is cooled to 30 DEG C with the speed of 0.7 DEG C/min, continue static fermentation 18h, fermentation liquid successively through 40 orders, 80 order duplex strainers filter, filtrate through superhigh-voltage homogenizing machine in 3 DEG C, 150MPa homogenizing breaking cellular wall obtain homogenizing fluid;

Described activity extra dry red wine yeast powder is prepared with ocean rhodotorula CCTCCNo:M2014592 according to a conventional method for the bacterium that sets out;

The protective agent used in described activity extra dry red wine yeast powder preparation process is step 2) the middle protective agent prepared;

4) breakdown of emulsion, separation and Extraction: homogenizing fluid is put 7000r/min traditional vacuum 12min in vacuum centrifuge, separate from top to bottom and obtain free oil, emulsion, hydrolyzed solution and solid residue, the emulsion obtained is controlled temperature is 40 DEG C, add the biological demulsifying agent breakdown of emulsion 50min of emulsion quality 0.02%, free oil and emulsion is obtained in 2500r/min traditional vacuum 10min again after breakdown of emulsion, the free oil merging gained separately uses it for anything else, and gained emulsion is extracted, separate, obtain finished product carotenoid after purification;

Described traditional vacuum condition is temperature 15 DEG C, vacuum-0.02MPa;

The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=4:3:2;

The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=5:1.5.

Cellular biomass in fermentation liquid prepared by said method and in emulsion the carrying out of carotenoid detect: cellular biomass is 52g/L; Carotenoid output is 61mg/L.

Embodiment 2

A kind of method utilizing inferior yellow peach fruit pulp fermentation to produce carotenoid, comprises the steps:

1) juice preparation: first by inferior yellow peach sarcocarp section, put in microwave dryer in power 3kW, thickness of feed layer 2cm, 105 DEG C, dry 2min, then 10min in the sericin peptide taken solution that mass percent is 10% it is immersed in, pulverize immediately after-18 DEG C of freezing 20min, freezing thickness of feed layer 3cm, ground product particle diameter 0.3mm, it is subsequently added into the water of ground product quality 1 times, it is 3.5 with breast acid for adjusting pH value, at electric field intensity 25kV/cm under room temperature, burst length 300 �� s, carries out high voltage pulse electric field processing 20min when pulse frequency 200Hz;Then carry out microwave irradiation and extraction 15min when power 150W in room temperature, simultaneously at power 200W, when frequency 30KHz, carry out ultrasonic assistant extraction; Add the mixed enzyme of extracting solution quality 3%, in 40 DEG C of enzymolysis 30min, filter, obtain fruit juice;

Described mixed enzyme is cellulase, protease, pectase, tannase 2:2:1:1 Homogeneous phase mixing in mass ratio;

2) prepared by fermentation medium: to step 1) fruit juice prepared adds the protective agent of its quality 5%, 1% sucrose, 0.5% ammonium sulfate, the potassium dihydrogen phosphate of 2%, the calcium chloride of 0.5% and 0.5% magnesium sulfate obtain fermentation medium;

Described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, the Radix Astragali, Radix Codonopsis, Herba Epimedii, Herba Houttuyniae is respectively washed, drain, 8:5:4:3:2:1 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 3h that pH value is 3.8 of mixed material quality 0.1 times, pulverize immediately after-18 DEG C of freezing 1h, freezing thickness of feed layer 3cm, ground product particle diameter 0.5mm, it is subsequently added into the water of ground product quality 10 times, it is 3.5 with breast acid for adjusting pH value, at electric field intensity 25kV/cm under room temperature, burst length 300 �� s, high voltage pulse electric field processing 20min is carried out when pulse frequency 200Hz, then microwave irradiation and extraction 15min is carried out in room temperature when power 150W, wherein, microwave exposure 10s, interval 20s, simultaneously at power 200W, when frequency 30KHz, carry out ultrasonic assistant extraction, add the compound enzyme of extracting solution quality 1%, in 45 DEG C of enzymolysis 30min, enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.1mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 3:2:1:1:1 Homogeneous phase mixing in mass ratio;

3) fermentation, homogenizing breaking cellular wall: to step 2) the active extra dry red wine yeast powder that adds its quality 0.2% in fermentation medium controls temperature 30 DEG C, speed of agitator 50r/min, ferment at constant temperature 12h, then with the ramp of 0.8 DEG C/min to 40 DEG C, add the active extra dry red wine yeast powder constant temperature static fermentation 3h of fermentation medium quality 0.1%, finally it is cooled to 28 DEG C with the speed of 0.6 DEG C/min, continue static fermentation 15h, fermentation liquid successively through 40 orders, 80 order duplex strainers filter, filtrate through superhigh-voltage homogenizing machine in 2 DEG C, 145MPa homogenizing breaking cellular wall obtain homogenizing fluid;

Described activity extra dry red wine yeast powder is prepared with ocean rhodotorula CCTCCNo:M2014592 according to a conventional method for the bacterium that sets out;

The protective agent used in described activity extra dry red wine yeast powder preparation process is step 2) the middle protective agent prepared;

4) breakdown of emulsion, separation and Extraction: homogenizing fluid is put 6000r/min traditional vacuum 10min in vacuum centrifuge, separate from top to bottom and obtain free oil, emulsion, hydrolyzed solution and solid residue, the emulsion obtained is controlled temperature is 30 DEG C, add the biological demulsifying agent breakdown of emulsion 40min of emulsion quality 0.01%, free oil and emulsion is obtained in 2000r/min traditional vacuum 8min again after breakdown of emulsion, the free oil merging gained separately uses it for anything else, and gained emulsion is extracted, separate, obtain finished product carotenoid after purification;

Described traditional vacuum condition is temperature 12 DEG C, vacuum-0.01MPa;

The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=3:2:1;

The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=4:1.

Cellular biomass in fermentation liquid prepared by said method and in emulsion the carrying out of carotenoid detect: cellular biomass is 51g/L; Carotenoid output is 58mg/L.

Embodiment 3

A kind of low-carbon (LC) produces the method for carotenoid, comprises the steps:

1) juice preparation: first by inferior for yellow peach sarcocarp section, then with yellow peach peels 3:1 Homogeneous phase mixing in mass ratio, put in microwave dryer in power 5kW, thickness of feed layer 4cm, 115 DEG C, dry 4min, then 15min in the sericin peptide taken solution that mass percent is 14% it is immersed in, pulverize immediately after-22 DEG C of freezing 40min, freezing thickness of feed layer 5cm, ground product particle diameter 0.5mm, it is subsequently added into the water of ground product quality 3 times, it is 5.5 with breast acid for adjusting pH value, at electric field intensity 35kV/cm under room temperature, burst length 500 �� s, high voltage pulse electric field processing 30min is carried out when pulse frequency 300Hz, then carry out microwave irradiation and extraction 20min when power 300W in room temperature, simultaneously at power 300W, when frequency 40KHz, carry out ultrasonic assistant extraction, add the mixed enzyme of extracting solution quality 5%, in 50 DEG C of enzymolysis 50min, filter, obtain fruit juice,

Described mixed enzyme is cellulase, protease, pectase, tannase 4:4:3:3 Homogeneous phase mixing in mass ratio;

2) prepared by fermentation medium: to step 1) fruit juice prepared adds the protective agent of its quality 9%, 3% sucrose, 1.5% ammonium sulfate, the potassium dihydrogen phosphate of 4%, the calcium chloride of 1.5% and 1.5% magnesium sulfate obtain fermentation medium;

Described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, the Radix Astragali, Radix Codonopsis, Herba Epimedii, Herba Houttuyniae is respectively washed, drain, 10:7:6:5:4:3 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 8h that pH value is 4.5 of mixed material quality 1 times, pulverize immediately after-22 DEG C of freezing 2h, freezing thickness of feed layer 5cm, ground product particle diameter 3mm, it is subsequently added into the water of ground product quality 20 times, it is 5.5 with breast acid for adjusting pH value, at electric field intensity 35kV/cm under room temperature, burst length 500 �� s, high voltage pulse electric field processing 30min is carried out when pulse frequency 300Hz, then microwave irradiation and extraction 20min is carried out in room temperature when power 300W, wherein, microwave exposure 10s, interval 20s, simultaneously at power 300W, when frequency 40KHz, carry out ultrasonic assistant extraction, add the compound enzyme of extracting solution quality 2%, in 55 DEG C of enzymolysis 50min, enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.3mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 5:4:3:3:2 Homogeneous phase mixing in mass ratio;

3) fermentation, homogenizing breaking cellular wall: to step 2) the active extra dry red wine yeast powder that adds its quality 0.4% in fermentation medium controls temperature 34 DEG C, speed of agitator 100r/min, ferment at constant temperature 15h, then with the ramp of 1.0 DEG C/min to 42 DEG C, add the active extra dry red wine yeast powder constant temperature static fermentation 5h of fermentation medium quality 0.3%, finally it is cooled to 32 DEG C with the speed of 0.8 DEG C/min, continue static fermentation 20h, fermentation liquid successively through 40 orders, 80 order duplex strainers filter, filtrate through superhigh-voltage homogenizing machine in 4 DEG C, 155MPa homogenizing breaking cellular wall obtain homogenizing fluid;

Described activity extra dry red wine yeast powder is prepared with ocean rhodotorula CCTCCNo:M2014592 according to a conventional method for the bacterium that sets out;

The protective agent used in described activity extra dry red wine yeast powder preparation process is step 2) the middle protective agent prepared;

4) breakdown of emulsion, separation and Extraction: homogenizing fluid is put 8000r/min traditional vacuum 15min in vacuum centrifuge, separate from top to bottom and obtain free oil, emulsion, hydrolyzed solution and solid residue, the emulsion obtained is controlled temperature is 50 DEG C, add the biological demulsifying agent breakdown of emulsion 60min of emulsion quality 0.03%, free oil and emulsion is obtained in 3000r/min traditional vacuum 12min again after breakdown of emulsion, the free oil merging gained separately uses it for anything else, and gained emulsion is extracted, separate, obtain finished product carotenoid after purification;

Described traditional vacuum condition is temperature 18 DEG C, vacuum-0.03MPa;

The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=5:4:3;

The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=6:2.

Cellular biomass in fermentation liquid prepared by said method and in emulsion the carrying out of carotenoid detect: cellular biomass is 45g/L; Carotenoid output is 52mg/L.

Embodiment 4

A kind of method utilizing yellow peach peels fermenting and producing carotenoid, comprises the steps:

1) juice preparation: first yellow peach peels is put in microwave dryer in power 3kW, thickness of feed layer 4cm, 105 DEG C, dry 4min, then 15min in the sericin peptide taken solution that mass percent is 10% it is immersed in, pulverize immediately after-18 DEG C of freezing 40min, freezing thickness of feed layer 3cm, ground product particle diameter 0.5mm, it is subsequently added into the water of ground product quality 1 times, it is 5.5 with breast acid for adjusting pH value, at electric field intensity 25kV/cm under room temperature, burst length 500 �� s, carries out high voltage pulse electric field processing 30min when pulse frequency 200Hz; Then carry out microwave irradiation and extraction 20min when power 150W in room temperature, simultaneously at power 200W, when frequency 40KHz, carry out ultrasonic assistant extraction; Add the mixed enzyme of extracting solution quality 3%, in 50 DEG C of enzymolysis 30min, filter, obtain fruit juice;

Described mixed enzyme is cellulase, protease, pectase, tannase 2:4:1:3 Homogeneous phase mixing in mass ratio;

2) prepared by fermentation medium: to step 1) fruit juice prepared adds the protective agent of its quality 5%, 3% sucrose, 0.5% ammonium sulfate, the potassium dihydrogen phosphate of 4%, the calcium chloride of 0.5% and 1.5% magnesium sulfate obtain fermentation medium;

Described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, the Radix Astragali, Radix Codonopsis, Herba Epimedii, Herba Houttuyniae is respectively washed, drain, 8:7:4:5:2:3 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 3h that pH value is 4.5 of mixed material quality 0.1 times, pulverize immediately after-22 DEG C of freezing 1h, freezing thickness of feed layer 3cm, ground product particle diameter 3mm, it is subsequently added into the water of ground product quality 10 times, it is 5.5 with breast acid for adjusting pH value, at electric field intensity 25kV/cm under room temperature, burst length 500 �� s, high voltage pulse electric field processing 30min is carried out when pulse frequency 200Hz, then carry out microwave irradiation and extraction 20min when power 150W in room temperature, simultaneously at power 200W, when frequency 40KHz, carry out ultrasonic assistant extraction, add the compound enzyme of extracting solution quality 1%, in 55 DEG C of enzymolysis 30min, enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.3mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 3:4:1:3:1 Homogeneous phase mixing in mass ratio;

3) fermentation, homogenizing breaking cellular wall: to step 2) the active extra dry red wine yeast powder that adds its quality 0.2% in fermentation medium controls temperature 34 DEG C, speed of agitator 50r/min, ferment at constant temperature 15h, then with the ramp of 0.8 DEG C/min to 42 DEG C, add the active extra dry red wine yeast powder constant temperature static fermentation 5h of fermentation medium quality 0.1%, finally it is cooled to 28 DEG C with the speed of 0.6 DEG C/min, continue static fermentation 20h, fermentation liquid successively through 40 orders, 80 order duplex strainers filter, filtrate through superhigh-voltage homogenizing machine in 2 DEG C, 155MPa homogenizing breaking cellular wall obtain homogenizing fluid;

4) breakdown of emulsion, separation and Extraction: homogenizing fluid is put 6000r/min traditional vacuum 15min in vacuum centrifuge, separate from top to bottom and obtain free oil, emulsion, hydrolyzed solution and solid residue, the emulsion obtained is controlled temperature is 30 DEG C, add the rhamnolipid breakdown of emulsion 40min of emulsion quality 0.03%, free oil and emulsion is obtained in 3000r/min traditional vacuum 8min again after breakdown of emulsion, the free oil merging gained separately uses it for anything else, and gained emulsion is extracted, separate, obtain finished product carotenoid after purification.

Cellular biomass in fermentation liquid prepared by said method and in emulsion the carrying out of carotenoid detect: cellular biomass is 42g/L; Carotenoid output is 48mg/L.

Embodiment 5

A kind of method utilizing yellow peach peels fermenting and producing carotenoid, comprises the steps:

1) juice preparation: first yellow peach peels is put in microwave dryer in power 5kW, thickness of feed layer 2cm, 115 DEG C, dry 2min, then 10min in the sericin peptide taken solution that mass percent is 14% it is immersed in, pulverize immediately after-22 DEG C of freezing 20min, freezing thickness of feed layer 5cm, ground product particle diameter 0.3mm, it is subsequently added into the water of ground product quality 3 times, it is 3.5 with breast acid for adjusting pH value, at electric field intensity 35kV/cm under room temperature, burst length 300 �� s, carries out high voltage pulse electric field processing 20min when pulse frequency 300Hz; Then carry out microwave irradiation and extraction 15min when power 300W in room temperature, simultaneously at power 300W, when frequency 30KHz, carry out ultrasonic assistant extraction; Add the mixed enzyme of extracting solution quality 5%, in 40 DEG C of enzymolysis 50min, filter, obtain fruit juice;

Described mixed enzyme is cellulase, protease, pectase, tannase 4:2:3:1 Homogeneous phase mixing in mass ratio;

2) prepared by fermentation medium: to step 1) fruit juice prepared adds the protective agent of its quality 9%, 1% sucrose, 1.5% ammonium sulfate, the potassium dihydrogen phosphate of 2%, the calcium chloride of 1.5% and 0.5% magnesium sulfate obtain fermentation medium;

Described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, the Radix Astragali, Radix Codonopsis, Herba Epimedii, Herba Houttuyniae is respectively washed, drain, 10:5:6:3:4:1 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 8h that pH value is 3.8 of mixed material quality 1 times, pulverize immediately after-18 DEG C of freezing 2h, freezing thickness of feed layer 3cm, ground product particle diameter 3mm, it is subsequently added into the water of ground product quality 10 times, it is 5.5 with breast acid for adjusting pH value, at electric field intensity 25kV/cm under room temperature, burst length 500 �� s, high voltage pulse electric field processing 30min is carried out when pulse frequency 200Hz, then carry out microwave irradiation and extraction 20min when power 150W in room temperature, simultaneously at power 200W, when frequency 40KHz, carry out ultrasonic assistant extraction, add the compound enzyme of extracting solution quality 1%, in 55 DEG C of enzymolysis 30min,Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.3mm obtains protective agent;

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 5:2:3:1:2 Homogeneous phase mixing in mass ratio;

3) fermentation, homogenizing breaking cellular wall: to step 2) the active extra dry red wine yeast powder that adds its quality 0.4% in fermentation medium controls temperature 30 DEG C, speed of agitator 100r/min, ferment at constant temperature 12h, then with the ramp of 1.0 DEG C/min to 40 DEG C, add the active extra dry red wine yeast powder constant temperature static fermentation 3h of fermentation medium quality 0.3%, finally it is cooled to 28 DEG C with the speed of 0.8 DEG C/min, continue static fermentation 20h, fermentation liquid successively through 40 orders, 80 order duplex strainers filter, filtrate through superhigh-voltage homogenizing machine in 4 DEG C, 145MPa homogenizing breaking cellular wall obtain homogenizing fluid;

4) breakdown of emulsion, separation and Extraction: homogenizing fluid is put 8000r/min traditional vacuum 10min in vacuum centrifuge, separate from top to bottom and obtain free oil, emulsion, hydrolyzed solution and solid residue, the emulsion obtained is controlled temperature is 50 DEG C, add the cell wall of emulsion quality 0.01% in conjunction with class biological demulsifying agent breakdown of emulsion 60min, free oil and emulsion is obtained in 2000r/min traditional vacuum 12min again after breakdown of emulsion, the free oil merging gained separately uses it for anything else, and gained emulsion is extracted, separate, obtain finished product carotenoid after purification.

Cellular biomass in fermentation liquid prepared by said method and in emulsion the carrying out of carotenoid detect: cellular biomass is 38g/L; Carotenoid output is 47mg/L.

Claims (10)

1. the method that a low-carbon (LC) produces carotenoid, it is characterised in that comprise the steps:

1) juice preparation: first Yellow-peach can leftover bits and pieces is put in microwave dryer in power 3-5kW, thickness of feed layer 2-4cm, 105-115 DEG C, dry 2-4min, then 10-15min in the sericin peptide taken solution that mass percent is 10-14% it is immersed in, pulverize immediately after-18--22 DEG C of freezing 20-40min, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.3-0.5mm, it is subsequently added into the water of ground product quality 1-3 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 �� s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz, then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction, add the mixed enzyme of extracting solution quality 3-5%, in 40-50 DEG C of enzymolysis 30-50min, filter, obtain fruit juice,

Described mixed enzyme is cellulase, protease, pectase, tannase 2-4:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio;

2) prepared by fermentation medium: to step 1) fruit juice prepared adds the protective agent of its quality 5-9%, the sucrose of 1-3%, the ammonium sulfate of 0.5-1.5%, the potassium dihydrogen phosphate of 2-4%, the calcium chloride of 0.5-1.5% and the magnesium sulfate of 0.5-1.5% obtain fermentation medium;

Described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, the Radix Astragali, Radix Codonopsis, Herba Epimedii, Herba Houttuyniae is respectively washed, drain, 8-10:5-7:4-6:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 3-8h that pH value is 3.8-4.5 of mixed material quality 0.1-1 times, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.5-3mm, it is subsequently added into the water of ground product quality 10-20 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 �� s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz,Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction; Add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min; Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent;

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 3-5:2-4:1-3:1-3:1-2 Homogeneous phase mixing in mass ratio;

3) fermentation, homogenizing breaking cellular wall: to step 2) the active extra dry red wine yeast powder that adds its quality 0.2-0.4% in fermentation medium controls temperature 30-34 DEG C, speed of agitator 50-100r/min, ferment at constant temperature 12-15h, then with the ramp of 0.8-1.0 DEG C/min to 40-42 DEG C, add the active extra dry red wine yeast powder constant temperature static fermentation 3-5h of fermentation medium quality 0.1-0.3%, finally it is cooled to 28-32 DEG C with the speed of 0.6-0.8 DEG C/min, continue static fermentation 15-20h, fermentation liquid is successively through 40 orders, 80 order duplex strainers filter, filtrate through superhigh-voltage homogenizing machine in 2-4 DEG C, 145-155MPa homogenizing breaking cellular wall obtains homogenizing fluid,

4) breakdown of emulsion, separation and Extraction: homogenizing fluid is put 6000-8000r/min traditional vacuum 10-15min in vacuum centrifuge, separate from top to bottom and obtain free oil, emulsion, hydrolyzed solution and solid residue, the emulsion obtained is controlled temperature and is 30-50 DEG C, add the biological demulsifying agent breakdown of emulsion 40-60min of emulsion quality 0.01-0.03%, free oil and emulsion is obtained in 2000-3000r/min traditional vacuum 8-12min again after breakdown of emulsion, the free oil merging gained separately uses it for anything else, and gained emulsion is extracted, separate, obtain finished product carotenoid after purification.

2. the method that a kind of low-carbon (LC) as claimed in claim 1 produces carotenoid, it is characterised in that step 1) described Yellow-peach can leftover bits and pieces is: any one or two kinds in yellow peach peels, inferior yellow peach sarcocarp mix with any mass ratio.

3. a kind of low-carbon (LC) as claimed in claim 1 produce carotenoid method, it is characterised in that step 2) described microwave irradiation and extraction be batch (-type) extract, i.e. microwave exposure 10s, interval 20s.

4. a kind of low-carbon (LC) as claimed in claim 1 produce carotenoid method, it is characterised in that step 3) described activity extra dry red wine yeast powder be prepare according to a conventional method with ocean rhodotorula CCTCCNo:M2014592 for the bacterium that sets out.

5. the method that a kind of low-carbon (LC) as claimed in claim 4 produces carotenoid, it is characterised in that the protective agent used in described activity extra dry red wine yeast powder preparation process is step 2) prepare.

6. the method that a kind of low-carbon (LC) as claimed in claim 1 produces carotenoid, it is characterised in that step 4) described traditional vacuum condition is temperature 12-18 DEG C, vacuum-0.01--0.03MPa.

7. the method that a kind of low-carbon (LC) as claimed in claim 1 produces carotenoid, it is characterised in that step 4) described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall are in conjunction with the one or more combination in class biological demulsifying agent.

8. the method that a kind of low-carbon (LC) as claimed in claim 7 produces carotenoid, it is characterised in that step 4) quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=3-5:2-4:1-3.

9. the method that a kind of low-carbon (LC) as claimed in claim 7 or 8 produces carotenoid, it is characterised in that described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl polyglucoside.

10. the method that a kind of low-carbon (LC) as claimed in claim 9 produces carotenoid, it is characterised in that the quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=4-6:1-2.