CN105368570A - Flavored bitter almond oil and extraction method thereof - Google Patents

Flavored bitter almond oil and extraction method thereof Download PDF

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CN105368570A
CN105368570A CN201510740407.2A CN201510740407A CN105368570A CN 105368570 A CN105368570 A CN 105368570A CN 201510740407 A CN201510740407 A CN 201510740407A CN 105368570 A CN105368570 A CN 105368570A
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oil
semen armeniacae
armeniacae amarum
local flavor
biological demulsifying
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邵素英
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C11B1/106—Production of fats or fatty oils from raw materials by extracting using ultra-sounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/16—Refining fats or fatty oils by mechanical means
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B5/00—Preserving by using additives, e.g. anti-oxidants

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  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Mechanical Engineering (AREA)
  • Biochemistry (AREA)
  • Cosmetics (AREA)

Abstract

The invention discloses flavored bitter almond oil and an extraction method thereof. The flavoured bitter almond oil uses bitter almonds as raw materials, uses low temperature and biological processing filed, effectively prevents heat-sensitive nutrients in the bitter almonds such as proteins, higher aliphatic acids and vitamins and the like from losing, and prepares free oil, emulsions, hydrolysate and almond cakes after microwave irradiation enzyme, frozen-breaking, enzymatic hydrolysis and traditional vacuum, the emulsion is centrifuged in vacuum to obtain the free oil after being biologically broken, and the free oil is combined to prepare a bitter almond oil which is high in extraction yield of oil (93%-97%), high in nutrition and active material content, strong in food safety (cyanide residue is below 0.08mg/kg ) and long in shelf life (5-6 years). The method for extracting the flavored bitter almond oil improves quality and yield, improves kinds, yield and additional value of by-products after greases are extracted, further reduces extraction cost of greases, puts an end to emission of three wastes, and protects environment.

Description

A kind of local flavor Semen Armeniacae Amarum oil and extracting method thereof
Technical field
The present invention relates to vegetable oil extraction technical field, be specifically related to a kind of local flavor Semen Armeniacae Amarum oil and extracting method thereof.
Background technology
Almond is the seed of Rosaceae cherry apricot, another name amygdala, apricot, wood are begun, Semen Armeniacae Amarum, Xing Meiren, sweet plum etc., in flat heart, surperficial yellowish brown is to dark-brown, and seed coat is thin, plants benevolence to be creamy white, High oleic.The ground such as Hebei, Liaoning, northeast, North China and Gansu are mainly distributed in China.Almond is divided into Sweet Apricot seed and Semen Armeniacae Amarum two class.Sweet Apricot seed taste is sweet, and do not contain or only contain the amygdaloside of 0.1%, lipid content reaches 45% ~ 67%.Semen Armeniacae Amarum bitter, containing 2% ~ 4% amygdaloside.Prunus amygdalus oil is the marrow of almond, in Semen Armeniacae Amarum oil, oleic acid and linoleic content reach more than 90%, its mid-oleic reaches 68%, be only second to sweet oil (80-82%) and tea oil (79-80%), almond is one of dietotherapeutic 27 main kinds of kind of health ministry first batch of promulgation in 1987.The scientific appraisal of foundation modern medicine and trophology: the nutrition of Prunus amygdalus oil is extremely abundant, easy absorption, and can not deposit in the blood vessel, long-term edible Prunus amygdalus oil, not only be of value to the cerebrovascular and intelligent growth, and the triglyceride level that can reduce in human body, there is anti-inflammatory, antianaphylaxis, anti-bacteria, suppression virus, prevent and treat hepatopathy, hypotensive, reducing blood-fat, prevent thrombosis, reduce vascular fragility, strengthen immunity, improve cardiovascular and cerebrovascular blood circulation, medicinal, health-care effect that the high and canceration of prevention three etc. is unique.The micro-Huang of Prunus amygdalus oil is transparent, fragrant taste, is not only a kind of excellent edible oil, or a kind of senior lubricating oil, can the low temperature of resistance to less than-20 DEG C, all has wide practical use in industries such as foodstuffs industry, medicine industry, makeup.
At present, the extracting method of Prunus amygdalus oil is a lot of, mainly contains low-temp extraction method, low temperature pressing method, soxhlet extraction, supercritical CO 2extraction process, microwave or ultrasound assisted extraction method, aqueous enzymatic method etc., or several method is wherein combined.Due to low-temp extraction method, soxhlet extraction, supercritical CO in numerous extracting method 2the methods such as extraction process all adopt organic solvent that grease is dissolved, extracted from the almond raw material processed, then slough solvent again, not only complex process, cycle is long, high to equipment and process requirement, and there is higher environmental hazard and food safety hidden danger, especially not easily apply in industries such as foodstuffs industry, medicine industry, makeup.Although traditional milling process quality is higher, oil yield is lower, and raw material availability is low, labour intensity is high, energy consumption is high, cost is high.
In 2l century, people pay attention to further to food safety, and national correlation department can be stricter to grease technical requirements standard.Tradition puies forward oily mode the defect that cannot break through at secure context.Along with the progress of science and technology, oil prodution industry is towards green production grease and the future development making full use of protein resource and lipoid and derivative thereof.After the nineties in last century, along with the suitability for industrialized production of enzyme, reduce the price of enzyme reagent, and traditional oil extracting process exists serious problem, domestic and international scientist carries out the research of aqueous enzymatic extraction.Aqueous enzymatic method carries that oily required equipment is simple, production safety, low stain; grease obtainedly be easy to refining; and oil extraction by enzymatic processing processing condition are gentle; available protecting grease, protein and colloid etc. can utilize the quality of composition, are conducive to the comprehensive utilization realizing grease and protein like this, improve the benefit of oil prodution industry; simultaneously; the transparency of gained Prunus amygdalus oil is high, raciness, and acid value, peroxide value are all low, long quality-guarantee period.
Grease is normally present in oilseeds cell with the form of lipopolysaccharides and lipoprotein, and lipopolysaccharides and lipoprotein are made up of grease and protein and carbohydrate etc., and the cell walls parcel that skin is made up of Mierocrystalline cellulose, hemicellulose, xylogen and pectin.First aqueous enzymatic method carries oil tech is the cellularstructure of oil plant tissue and grease compounded body are destroyed by Mechanical Crushing, on the basis of release grease wherein, cellulase, hemicellulase, proteolytic enzyme, polygalacturonase, amylase, dextranase etc. is adopted to degrade to oil plant tissue and to the complex body such as lipopolysaccharides, lipoprotein, destroy cellularstructure, increase the mobility of oil in oil plant tissue, thus make oil free out, and protein can be reclaimed.From the seventies in last century, along with the suitability for industrialized production of zymin, greatly reduce the price of enzyme, establish important basis for scientist studies application enzyme extraction grease.Study aqueous enzymatic extraction research so far both at home and abroad and relate to the oil crops such as peanut, maize germ, soybean, Semen Brassicae campestris, tea seed, rice bran.Aqueous enzymatic extraction tea seed grease realizes suitability for industrialized production, achieve edible oil processing need not squeeze, without organic solvent extraction, without refining, need not any additive, be green grease production technique.Sheng little Na etc. have studied the operational path of the stepwise discretization utilizing composite plant lytic enzyme, polygalacturonase and Alcalase Sumizyme MP, and oil yield finally can reach 72.58%.Concrete optimised process is: solid-to-liquid ratio l:5, composite plant lytic enzyme and polygalacturonase 1:2 composite, enzyme add total amount 2.5%, enzymolysis time 5h; Alkali carries pH8.5, and alkali carries temperature 60 C, and alkali carries time 60min; The initial pH10 of Alcalase proteolytic enzyme, temperature of reaction 60 DEG C, concentration 1.5%, reaction times 4h.Final free oil yield is 72.58%, yield of aminosal be 82.35% (Sheng little Na, Wang Zhang, Xu Shiying. the research of aqueous enzymatic extraction sweet almond oil and protolysate. Chinese oil, 2007,32 (11): 26-30.); Ma Yan etc. utilize the composite of cellulase and neutral protease, show that the optimal processing parameter of aqueous enzymatic extraction Sweet Apricot seed is: material order number is 60 orders, cellulase+neutral protease (2:3) is selected to carry out composite, enzymolysis pH is 7, enzymolysis time is 3.03h, hydrolysis temperature is 55.05 DEG C, when enzyme addition is 3.17%, it is 56.24% that enzymolysis process extracts sweet almond oil extraction yield (horse swallow. the extraction process of sweet almond oil and quality research. Urumchi: Xinjiang University, 2010.); He Yutang etc. utilize the composite of Sumizyme MP and neutral protease, and oil extracting rate reaches 39.88%.Concrete optimised process is: solid-liquid ratio is l:5.3, Sumizyme MP 1.1%, neutral protease O.9%, enzymolysis time 132min (Sumizyme MP and neutral protease are respectively 66min) (He Yutang, Wang Li, Liu He, etc. response phase method optimizes sweet almond oil extraction process. foodstuffs industry science and technology, 2012,33 (1): 217-219.); Tian Honglei etc. adopt neutral protease to carry out being hydrolyzed extracting to Xinjiang little white apricot almond grease, and have carried out gas chromatographic analysis to the lipid acid composition extracting grease.Main enzymolysis pH value, temperature, reaction times and enzyme dosage 4 factors inquired into are on the impact of grease extraction efficiency.Research shows: when neutral protease consumption is 2000IU/g almond, enzymolysis pH7.5, under the condition of temperature 60 C, after enzymolysis 2h, and the total extraction rate reached 88.8l% of almond grease; The gas-chromatography fatty acid analysis of Prunus amygdalus oil shows: almond oil forms primarily of unsaturated fatty acids, 91.07% of grease total amount can be reached, wherein be mainly oleic acid and linolic acid, content is respectively 40.92% and 49.30% (Tian Honglei, Zhan Ping, Luo Ling. the Enzymatic Extraction of almond oil and fatty acid compositional analysis, Shihezi Univ's journal natural science edition, 26th volume the 6th phase, in December, 2008).
The critical process of aqueous enzymatic extraction grease is the degree of crushing of oil plant, the kind of enzyme and action condition, breakdown of emulsion, emulsification is due to protein tool lipophilic group and hydrophilic radical, interact with heterogeneous system Middle molecule such as profits, form water solution of oil, a skim is formed in oil droplet periphery, effectively prevent fat aggregation, impel oil and water to form emulsion, and keep emulsion stability.Stable emulsion significantly limit oil separating clearly, and breakdown of emulsion is more thorough, and free oil yield is higher, and economic worth is higher.So the breakdown of emulsion link that to be oil extraction by enzymatic processing technique the most key, the quality of breakdown of emulsion directly has influence on the economic worth of whole technique, determines aqueous enzymatic extraction and industrially whether applies.Now common breaking method is divided into chemical demulsification, physics breakdown of emulsion and biological demulsifying.Chemical demulsification method adds chemical demulsifier; Heating, centrifugal, change temperature, apply electrostatic field, adopt coalescing agent etc. to belong to physical method; Biological demulsifying method refers to and utilizes the meta-bolites of microorganism cells itself or its metabolic process to realize emulsion breakdown.Due to the existence of the organic solvent residual of chemical method and the problem such as the demulsification efficiency of Physical is low, select biological method as breakdown of emulsion means, have that processing unit is simple, cost is low, demulsification efficiency is high, waste liquid can be degraded, hypotoxicity, feature that pollution is few, and the full nutrient solution Heat stability is good of mixing demulsifying bacteria, reusable, under extreme conditions play the advantages such as active.
Sheng little Na etc. improve free oil yield further, relatively microwave, heating, freeze-thaw, this several physical mechanical method breakdown of emulsion standing, wherein freeze-thaw demulsification is best, 20h is freezed by milk sap-18 DEG C, then at 40 DEG C of 2h that thaw, the centrifugal 20min of 8000r/min, demulsification efficiency can reach 42.4% (Sheng little Na, Wang Zhang, Xu Shiying. the research of aqueous enzymatic extraction sweet almond oil and protolysate. Chinese oil, 2007,32 (11): 26-30.), still lower, have much room for improvement.
Chinese patent CN101736046B discloses a kind of biological method extracting grease from flat almond, flat almond is carried out dry milling, flat almond powder after pulverizing is mixed with water, regulate pH6.5 ~ 7.5, temperature 50 C, add the neutral protease of flat almond opaque amount 0.1% ~ 1.0%, enzymolysis 2 ~ 6 hours; Be cooled to 40 DEG C again, regulate pH to 7.5 ~ 8.5, add the Sumizyme MP of raw materials quality 0.1% ~ 1.0%, enzymolysis 2 ~ 6 hours, obtains enzymolysis solution, and enzymolysis solution is directly centrifugal, be separated from top to bottom and obtain free oil, milk sap, hydrolyzed solution and almond slag, the milk sap obtained is added the lipopeptid biological demulsifying agent accounting for milk sap volume 0.1 ~ 0.5%, after breakdown of emulsion, recentrifuge is separated and obtains free oil, and namely the free oil merging gained obtains flat almond oil.This invention adopts dry milling can avoid forming stable milk sap in shattering process, reduces the consumption of emulsion splitter, reduces costs.Prepare flat almond oil by biological method, squeezing grease can be avoided to cause the oxidation of fat, the problem avoiding lixiviation process to carry oil causing dissolvent residual.But dry milling shearing force is large, temperature is high, cause Prunus amygdalus oil Middle nutrition and biologically active substance loss greatly.
A kind of method that Chinese patent CN102796613B discloses aqueous enzymatic extraction soybean oil microbial de-emulsification belongs to Vegetable oil lipoprotein extractive technique, the method comprises the following steps: (1) soybean adopts expelling-expansion pretreatment to obtain expanded material after pulverizing, expanded material and water are mixed to get mixed solution, in mixed solution, add Sumizyme MP carry out enzymolysis, after enzymolysis, centrifugation obtains free oil, milk sap, hydrolyzed solution and residue; (2) mixing demulsifying bacteria to be fermented through MMSM inorganic salt liquid substratum with different compound proportion to obtain mixing demulsifying bacteria full nutrient solution; (3) by step 1) milk sap that obtains adjusts pH neutral, and mix the full nutrient solution of demulsifying bacteria and fully mixes, and standingly carries out constant temperature breakdown of emulsion, and after breakdown of emulsion, centrifugation obtains soybean oil; Although demulsification efficiency is higher, after broken emulsion centrifugation, grease is mixed with demulsifying bacteria and water, emulsus oil, demulsifying bacteria can not be separated completely with water, affect food safety and the stability of grease.
Chinese patent CN103785196B discloses a kind of method improving cell wall bound biodemulsifier demulsification performance, the method comprises the following steps: cell wall bound biodemulsifier is carried out lyophilize, obtain biological demulsifying agent dry powder, then biological demulsifying agent dry powder and a certain proportion of methyl alcohol and hydrochloric acid mixed solution mix and blend are carried out esterification reaction of organic acid, after eventually passing rotary evaporation, oven dry, obtain the cell wall bound biodemulsifier that demulsification performance improves.Above-mentioned technique introduces chemical toxic solvent, and emulsion splitter exists larger food safety hidden danger.
Chinese patent CN101589745B discloses a kind of detoxified almond oil production method, and the method for raw material, comprises broken shell step with bitter apricot seed: by the broken shell of bitter apricot seed, sort out Semen Armeniacae Amarum; Cold press step: by Semen Armeniacae Amarum cold press at ambient temperature, obtains Semen Armeniacae Amarum fatty oil and degreasing Semen Armeniacae Amarum; Extraction step: Semen Armeniacae Amarum fatty oil is put into container, adds the water of 4 ~ 8 times of weight, stirs into pasty state, centrifugation oil phase and aqueous phase; Extract 2 ~ 5 times with the water of its 4 ~ 8 times of weight for the oil phase be separated, finally separated oil phase is detoxified almond oil again.The detoxified almond oil of the detoxified almond oil production method production of gained, wherein residual prussiate is below 0.2mg/kg.The Semen Armeniacae Amarum oil extraction yield that aforesaid method obtains is lower.
To sum up, from the critical process affecting aqueous enzymatic extraction Prunus amygdalus oil, seek that a kind of grease extraction efficiency is high, nutrition and activity substance content is high, food safety strong and the Prunus amygdalus oil green extraction process of long quality-guarantee period is still the long-sought of those skilled in the art.
Summary of the invention
Technical problem solved by the invention is the defect overcoming existing Prunus amygdalus oil extraction process, take Semen Armeniacae Amarum as raw material, free oil, milk sap, hydrolyzed solution and almond slag is obtained after microwave exposure goes out enzyme, freezing crushing, enzymolysis, traditional vacuum, after milk sap is wherein carried out biological demulsifying, traditional vacuum obtains free oil again, free oil is merged, obtains that a kind of grease extraction efficiency is high, nutrition and activity substance content is high, food safety strong, the Semen Armeniacae Amarum oil of long quality-guarantee period.
In order to achieve the above object, the present invention is by the following technical solutions:
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
1) microwave exposure goes out enzyme: Semen Armeniacae Amarum is immersed water or mass percent concentration is room temperature rinsing in the sodium hydrogen carbonate solution of 0.05-0.15%, drains, put sealing and standing in container and soak 2-4h, then peel, decortication Semen Armeniacae Amarum is put into microwave dryer and to go out enzyme 2-4min in frequency 2450MHz, power 1000-3000W, temperature 80-90 DEG C, bed thickness 3-5cm condition microwave exposure;
Further, described microwave exposure is Batch irradiation;
Preferably, described Batch irradiation is: microwave exposure 10s, interval 20s;
2) freezing crushing: the Semen Armeniacae Amarum after microwave exposure goes out enzyme is soaking at room temperature 2-4h in the citric acid solution of 0.1-0.3% at water or mass percent concentration, clear water rinsing 1-3 time, drain, in-20--25 DEG C, the freezing 3-5h of bed thickness 8-10cm, be crushed to particle diameter 0.2-0.4mm and obtain Semen Armeniacae Amarum powder;
3) enzymolysis: adding its quality 6-8 pH value doubly in Semen Armeniacae Amarum powder is the solion of 3.5-5.5, Homogeneous phase mixing, and controlling mixture temperature is 45-55 DEG C, add the compound enzyme of Semen Armeniacae Amarum opaque amount 0.03-0.05% wherein, stir, insulation, enzymolysis 40-60min;
Further, described solion is the aqueous solution containing sodium ion 28-33mg/L, magnesium ion 20-25mg/L, zine ion 20-25mg/L, potassium ion 18-23mg/L and calcium ion 12-14mg/L;
Further, the quality group of described compound enzyme becomes: aspartic protease: cellulase: polygalacturonase: beta-glucanase: amylase=10-15:2-4:1-3:1-3:1-2;
4) traditional vacuum: enzymolysis solution is put 6000-8000r/min traditional vacuum 5-8min in vacuum centrifuge, be separated from top to bottom and obtain free oil, milk sap, hydrolyzed solution and Semen Armeniacae Amarum slag, be 30-50 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent of milk sap quality 0.04-0.06% or the biological demulsifying bacteria culture fluid breakdown of emulsion 40-60min of 5-8%, after breakdown of emulsion in 2000-3000r/min again traditional vacuum 10-15min obtain free oil, merge the free oil of gained, add the natural antioxidants of its quality 0.01-0.03%, namely Homogeneous phase mixing obtains Semen Armeniacae Amarum oil,
Further, described traditional vacuum condition is temperature 10-20 DEG C, vacuum tightness-0.01--0.03MPa;
Further, described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall are in conjunction with the one or more combination in class biological demulsifying agent;
Preferably, the quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=6-10:5-7:3-5;
Preferably, described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl glycoside;
More preferably, the quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl glycoside=7-9:1-3;
Further, described biological demulsifying bacteria culture fluid be can produce biological demulsifying agent with one or more and the higher microbial strains (such as: Alcaligenes, Mo Haiwei genus bacillus, Bacillus subtillis and mud Nocardia bacteria etc.) of demulsification efficiency for starting strain, after conventional enlarged culturing, the viable bacteria content fermented in special liquid substratum is high, metabolite (biological demulsifying agent) measures large biological demulsifying fermented liquid;
Preferably, the preparation method of described biological demulsifying bacteria culture fluid is: inoculate 1 ring in 100mL broth culture by after the test tube slant actication of culture of Alcaligenes Alcaligenessp.S-XJ-1, 30-35 DEG C, 24-36h cultivated by 120-130r/min shaking table, then be inoculated in 2000mL seed culture medium, 30-35 DEG C, 36-48h cultivated by 110-120r/min shaking table, obtain seed liquor, seed liquor is inoculated in the inoculum size of volume ratio 7% in the airlift fermentor that 20L fermention medium is housed, in 35-40 DEG C, air flow 2-4L/min constant temperature open fermentation 48-60h, then 28-32 DEG C is cooled to the speed of 0.6-0.8 DEG C/h, seed liquor is continued to add inoculation with volume ratio 3% inoculum size, close tank fermentation 24-30h, keep tank pressure 0.01-0.03MPa, last with the ramp of 0.8-1.0 DEG C/h to 35-40 DEG C, namely air flow 1-3L/min constant temperature open fermentation 24-30h obtains biological demulsifying bacteria culture fluid,
Described broth culture consists of: extractum carnis 5.0g, peptone 10.0g, yeast extract paste 5.0g, NaCl5.0g, glucose 10.0g, distilled water 1L, pH value 7.0;
Described seed culture medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 1-3g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 2% with culture volume;
Described fermention medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 2-5g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 4% with culture volume;
Described Chinese herbal medicine extract significantly improves demulsifying bacteria to resist the herbal medicine of hot and cold irritability, disease resistance and immunizing power to prepare for raw material;
Preferably, the preparation method of described Chinese herbal medicine extract, comprises the steps: to count by weight, takes Radix Glycyrrhizae 15-17 part, Radix Astragali 13-16 part, Herba Epimedii 12-15 part, rhizoma zingiberis 12-15 part, root of Dahurain angelica 8-12 part, Radix Codonopsis 5-8 part; Put in the Ultrasonic Cleaners filling 0.1-0.3% sodium hydrogen carbonate solution and clean 10-15min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixture, control temperature 70-90 DEG C keeps 2-4h, then 40-50 DEG C is cooled to, be 3.5-4.5 by lactic acid adjust ph, microwave extraction 10-15min is carried out under power 150-300W condition, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic-assisted extraction simultaneously; Then the prozyme adding mixture gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixture 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature keeps 3-4h to 60-78 DEG C, filters, obtains the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, obtains Chinese herbal medicine extract;
Described prozyme is dextranase, pentosanase, zytase, tannase 2-4:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio.
Further, described natural antioxidants contains any one or a few in tea-polyphenol, grape pip procyanidin, rosemary extract, Radix Glycyrrhizae extract and apricot leaf extract;
Preferably, the quality group of described natural antioxidants becomes: tea-polyphenol: grape pip procyanidin: Vc=2-3:1-3:1-2.
Another object of the present invention is to provide the local flavor Semen Armeniacae Amarum oil that said extracted method obtains.
Beneficial effect:
The present invention take Semen Armeniacae Amarum as raw material, omnidistance employing low temperature, biological processing technology, effectively prevent the loss of the heat-sensitive nutrition such as protein in Semen Armeniacae Amarum, higher fatty acid, VITAMIN, especially the loss of the antioxidation biology such as unsaturated fatty acids and the VE active substance such as oleic acid, linolic acid, physetoleic acid, linolenic acid, fundamentally ensure that the physiological function of Semen Armeniacae Amarum oil and the stability of trophic function, extend the shelf-lives of product to 5-6.And for amygdalate property of raw material, adopt alkali lye rinsing, decortication, microwave deactivating enzyme, citric acid solution soaks and repeatedly the technique such as rinsing combines process raw material, effectively prevent the synthesis of toxic substance, the residual quantity significantly reducing prussiate in Semen Armeniacae Amarum oil, to below 0.08mg/kg, substantially increases the food safety of Semen Armeniacae Amarum oil.Adopt alkali lye to leave standstill the methods such as wetting, microwave drying, freezing crushing (ice crystal aculea wound is combined with mechanical effect), compound enzyme acid ion in-solution digestion simultaneously, make the combination oil in Semen Armeniacae Amarum and free oil stripping to greatest extent, and traditional vacuum and biological demulsifying will be adopted to organically combine, significantly improve the extraction yield of Semen Armeniacae Amarum oil to 93-97%, improve raw material availability, reduce extraction cost.Improve the quality of products and add while extraction yield grease extract after the kind of by product, output and added value; reduce further grease extraction cost; stop the discharge of " three wastes "; protect environment; really achieve low-carbon (LC) to produce, final obtained a kind of unsaturated fatty acids and nutrition and bioactive substance content is high, resistance of oxidation and stability is strong, food safety is high, the Semen Armeniacae Amarum oil of long quality-guarantee period.Specific experiment effect is shown in enforcement 13-17; Concrete know-why is as follows:
1. the present invention by sodium bicarbonate or water rinse, wettingly make Semen Armeniacae Amarum be beneficial to complete decortication, the more important thing is and pass through wetting action, make moisture content appropriateness uniform pickup in Semen Armeniacae Amarum, then through microwave drying, not only effectively eliminate the biological activity of harmful enzyme such as synaptase, polyphenoloxidase in Semen Armeniacae Amarum, prevent the synthesis of toxic substance; And make Semen Armeniacae Amarum appropriateness expanded, enrich the grid structure of Semen Armeniacae Amarum tissue, improve a histiocytic permeability, and by follow-up citric acid solution or water soaking, repeatedly rinsing, improve the exchange of substance ability of know clearly Semen Armeniacae Amarum organization internal and external agency, quick, effectively debitterize, detoxification further, the residual quantity significantly reducing prussiate in Semen Armeniacae Amarum oil, to below 0.08mg/kg, substantially increases the food safety of Semen Armeniacae Amarum oil.
2. Semen Armeniacae Amarum of the present invention is through pre-treatment and microwave drying, enrich amygdalate inner mesh structure, add the permeability of interior tissue, by soaking and rinsing, make the water-swelling of Semen Armeniacae Amarum appropriateness, then through freezing, ice crystal is formed at Semen Armeniacae Amarum inner homogeneous, stab histocyte, add follow-up Mechanical Crushing, make a large amount of strippings of nutritive substance such as the higher fatty acid of combined in Semen Armeniacae Amarum and free state, organically combine with biological enzymolysis, and traditional vacuum and biological demulsifying will be adopted to organically combine, significantly improve the extraction yield of Semen Armeniacae Amarum oil to 93-97%, improve raw material availability, improve quality product, reduce extraction cost.
3. biological enzymolysis of the present invention to dissociate mineral ions content according to Semen Armeniacae Amarum, using composite for the science acid ion solution containing many kinds of metal ions as enzymolysis matrix, the compound enzyme making science composite is reacted at optimum conditions, significantly have activated the center of enzyme molecule protein and the biological activity of peripheral structure, extraordinaryly excite bioenzyme activity, play the vigor of biological enzyme to greatest extent, thoroughly degrade lipopolysaccharides in Semen Armeniacae Amarum, lipoprotein and cell wall structure, make a large amount of strippings of nutritive substance such as the higher fatty acid of combined in Semen Armeniacae Amarum and free state, highly shortened enzymolysis time, reduce biological enzyme usage quantity, improve hydrolysis result.
4. the present invention through concentrating on studies for many years, scientific experimentation many times, by composite for dissimilar biological demulsifying agent science, its effect is better than the biological demulsifying agent of the single use of any one, and its demulsification efficiency can reach more than 96%.
5. biological demulsifying bacteria culture fluid of the present invention be can produce biological demulsifying agent with one or more and the higher microbial strains of demulsification efficiency for starting strain, spread cultivation and fermenting process substratum and zymotechnique by optimizing bacterial classification, segmentation is adopted to add the mode of inoculation and temperature-variable fermentation, shorten cell age and propagation algebraic difference distance, effectively improve biological activity and the fermenting power of demulsifying bacteria nutrient solution, and then improve the content of biological demulsifying bacteria and emulsion splitter in demulsifying bacteria nutrient solution, finally significantly improve more than the demulsification efficiency to 98% of milk sap, wherein seed culture medium and the composite Chinese herbal medicine extract of fermention medium science significantly improve bacterial classification to resist cold, heat stress, the herbal medicine of immunity and disease resistance is raw material, through ultrasonic cleaning, low temperature enzymolysis, prepared by water extraction alcohol extracting and ultrasonic assistant microwave extraction, extract active constituent content is high, cost is low, can effectively by taming step by step, rejuvenation enlarged culturing, enhance biological demulsifying bacteria high temperature resistance and Cold stress ability, improve the ability of producing the meta-bolitess such as biological demulsifying agent, excite its multiplication capacity and propagation density, shorten moderate phase and the decline phase of bacterial classification propagation, extend Exponential growth stage and stationary phase, effectively improve the density of demulsifying bacteria bacterial classification and the output of biological demulsifying agent, enhance the adaptability of bacterial strain to yeasting, enhance the anti-stress of demulsifying bacteria comprehensively, disease resistance and immunological competence, adopt ultrasonic cleaning effectively can kill worm's ovum in raw material and miscellaneous bacteria simultaneously, reduce heavy metal ion content and pesticide residue, improve the food safety of nutrient solution and the quality guaranteed period of liquid spawn.
6. extracting method of the present invention is simple, cycle is short, efficiency is high, save the energy, environmentally friendly, omnidistance employing low temperature, biological processing technology, effectively prevent the loss of the heat-sensitive nutrition such as protein in Semen Armeniacae Amarum, higher fatty acid, VITAMIN, improve the extraction yield of Semen Armeniacae Amarum oil and biological activity and nutrition content; And kinds of processes is combined decortication, detoxification, significantly reduces the residual quantity of prussiate in Semen Armeniacae Amarum oil, substantially increase the food safety of Semen Armeniacae Amarum oil; Be combined with biological demulsifying by traditional vacuum, the natural antioxidants that science is composite, interpolation is applicable to Semen Armeniacae Amarum oil, significantly improves the stability of Semen Armeniacae Amarum oil, extends the quality guaranteed period of product simultaneously.
It should be noted that technique effect of the present invention is the synergistic summation of each step technique feature, there is between each step certain inherent dependency, not the simple superposition of single technical characteristic effect.
Embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technique means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Vacuum centrifuge of the present invention is the high-speed vacuum refrigerated centrifuge that extensive and profound in meaning tower company of the U.S. produces, and product type is: HTC-30000I, maximum speed of revolution: 30000RPM, maximum centrifugal force: 101625 × g, maximum capacity: 1000ml × 6Tubes.
Alcaligenes Alcaligenessp.S-XJ-1 of the present invention is the demulsifying bacteria disclosed in Chinese patent CN101407769B, this bacterium is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on August 24th, 2007, and deposit number is CGMCCNO.2142.
Cell wall bound biodemulsifier of the present invention is the biological demulsifying agent disclosed in Chinese patent CN103785196B.
Grape pip procyanidin of the present invention is bought in Earthquake of Anyang station in Henan Jing Sen bio tech ltd, effective constituent: OPC/Polyphenol, AssayOPC95%/polyphenol90%.
Folium Ginkgo extract of the present invention is bought in Xuzhou Heng Kai ginkgo product company limited, Chinese Pharmacopoeia 2010 editions Folium Ginkgo extracts, total flavonoids >=24%, equipment registration trade mark: Gongsun's hall GinkgoTown.
Rosemary extract of the present invention buys the gloomy source book on Chinese herbal medicine natural product limited-liability company in Henan, main component: rosmarinic acid, rosmanol etc., content 85%, registered trademark gloomy source book on Chinese herbal medicine.
Radix Glycyrrhizae extract of the present invention is bought in Yuan Sen bio tech ltd, Xi'an, main component: Potenlini, Liquiritin etc., content 98%, the gloomy biology in registered trademark source.
Tea-polyphenol of the present invention buys the Green Tea Polyphenols produced in Rayleigh, Xi'an biotech firm, polyphenol content 98%, and registered trademark Rayleigh is biological.
Prepared by embodiment 1 raw material
1. the preparation of Chinese herbal medicine extract:
The preparation method of described Chinese herbal medicine extract, comprises the steps: to count by weight, takes 16 parts, Radix Glycyrrhizae, the Radix Astragali 15 parts, Herba Epimedii 14 parts, rhizoma zingiberis 13 parts, the root of Dahurain angelica 10 parts, Radix Codonopsis 6 parts; Put in the Ultrasonic Cleaners filling 0.2% sodium hydrogen carbonate solution and clean 12min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, puts Homogeneous phase mixing in container and adds the water of 5 times of weight, obtaining mixture, control temperature 80 DEG C keeps 3h, then being cooled to 45 DEG C, is 4.0 by lactic acid adjust ph, under power 200W condition, carry out microwave extraction 12min, under power 250W, frequency 35KHz condition, carry out ultrasonic-assisted extraction simultaneously; Then the prozyme adding mixture gross weight 1.0% carries out enzymolysis, is 6.2 by lactic acid adjust ph, enzymolysis 3h, finally add the mixture of mixture 2 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, and control temperature to 70 DEG C keeps 3h, filter, obtain the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 3:2, Homogeneous phase mixing, obtains Chinese herbal medicine extract;
Described prozyme is dextranase, pentosanase, zytase, tannase 3:3:2:2 Homogeneous phase mixing in mass ratio.
The Chinese herbal medicine extract that following examples use is embodiment 1 to be prepared, and other is commercial commodity.
Embodiment 2
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
1) microwave exposure goes out enzyme: Semen Armeniacae Amarum is immersed mass percent concentration and be room temperature rinsing in the sodium hydrogen carbonate solution of 0.1%, drain, put sealing and standing in container and soak 3h, then peel, decortication Semen Armeniacae Amarum is put into microwave dryer to go out enzyme 3min in frequency 2450MHz, power 2000W, temperature 85 DEG C, bed thickness 4cm condition microwave exposure, wherein microwave exposure 10s, interval 20s;
2) freezing crushing: the Semen Armeniacae Amarum after microwave exposure goes out enzyme is soaking at room temperature 3h in the citric acid solution of 0.2% at mass percent concentration, clear water rinsing 2 times, drains, and in-23 DEG C, the freezing 4h of bed thickness 9cm, is crushed to particle diameter 0.3mm and obtains Semen Armeniacae Amarum powder;
3) enzymolysis: the pH value adding its quality 7 times in Semen Armeniacae Amarum powder is the solion of 4.5, Homogeneous phase mixing, controlling mixture temperature is 50 DEG C, adds the compound enzyme of Semen Armeniacae Amarum opaque amount 0.04% wherein, stirs, insulation, enzymolysis 50min;
Described solion is the aqueous solution containing sodium ion 30mg/L, magnesium ion 22mg/L, zine ion 22mg/L, potassium ion 20mg/L and calcium ion 13mg/L;
The quality group of described compound enzyme becomes: aspartic protease: cellulase: polygalacturonase: beta-glucanase: amylase=13:3:2:2:1.5;
4) traditional vacuum: enzymolysis solution is put vacuum tightness-0.02MPa in vacuum centrifuge, 15 DEG C, 7000r/min traditional vacuum 6min, be separated from top to bottom and obtain free oil, milk sap, hydrolyzed solution and Semen Armeniacae Amarum slag, it is 40 DEG C by the milk sap control temperature obtained, add the biological demulsifying bacteria culture fluid breakdown of emulsion 50min of milk sap quality 6%, after breakdown of emulsion in vacuum tightness-0.02MPa, 15 DEG C, 2500r/min again traditional vacuum 12min obtain free oil, merge the free oil of gained, add the natural antioxidants of its quality 0.02%, namely Homogeneous phase mixing obtains Semen Armeniacae Amarum oil;
The preparation method of described biological demulsifying bacteria culture fluid is: inoculate 1 ring in 100mL broth culture by after the test tube slant actication of culture of Alcaligenes Alcaligenessp.S-XJ-1, 33 DEG C, 30h cultivated by 125r/min shaking table, then be inoculated in 2000mL seed culture medium, 33 DEG C, 42h cultivated by 115r/min shaking table, obtain seed liquor, seed liquor is inoculated in the inoculum size of volume ratio 7% in the airlift fermentor that 20L fermention medium is housed, in 37 DEG C, air flow 3L/min constant temperature open fermentation 54h, then 30 DEG C are cooled to the speed of 0.7 DEG C/h, seed liquor is continued to add inoculation with volume ratio 3% inoculum size, close tank fermentation 27h, keep tank pressure 0.02MPa, last with the ramp to 37 DEG C of 0.9 DEG C/h, namely air flow 2L/min constant temperature open fermentation 27h obtains biological demulsifying bacteria culture fluid,
Described broth culture consists of: extractum carnis 5.0g, peptone 10.0g, yeast extract paste 5.0g, NaCl5.0g, glucose 10.0g, distilled water 1L, pH value 7.0;
Described seed culture medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 2g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 2% with culture volume;
Described fermention medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 3.5g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 4% with culture volume;
The quality group of described natural antioxidants becomes: tea-polyphenol: grape pip procyanidin: Vc=2.5:2:1.5.
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 97%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.04mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 6 years; The demulsification efficiency of biological demulsifying is 99.3%.
Embodiment 3
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
1) microwave exposure goes out enzyme: Semen Armeniacae Amarum is immersed mass percent concentration and be room temperature rinsing in the sodium hydrogen carbonate solution of 0.05%, drain, put sealing and standing in container and soak 2h, then peel, decortication Semen Armeniacae Amarum is put into microwave dryer to go out enzyme 2min in frequency 2450MHz, power 1000W, temperature 80 DEG C, bed thickness 3cm condition microwave exposure, wherein microwave exposure 10s, interval 20s;
2) freezing crushing: the Semen Armeniacae Amarum after microwave exposure goes out enzyme is soaking at room temperature 2h in the citric acid solution of 0.1% at mass percent concentration, clear water rinsing 1 time, drains, and in-20 DEG C, the freezing 3h of bed thickness 8cm, is crushed to particle diameter 0.2mm and obtains Semen Armeniacae Amarum powder;
3) enzymolysis: the pH value adding its quality 6 times in Semen Armeniacae Amarum powder is the solion of 3.5, Homogeneous phase mixing, controlling mixture temperature is 45 DEG C, adds the compound enzyme of Semen Armeniacae Amarum opaque amount 0.03% wherein, stirs, insulation, enzymolysis 40min;
Described solion is the aqueous solution containing sodium ion 28mg/L, magnesium ion 20mg/L, zine ion 20mg/L, potassium ion 18mg/L and calcium ion 12mg/L;
The quality group of described compound enzyme becomes: aspartic protease: cellulase: polygalacturonase: beta-glucanase: amylase=10:2:1:1:1;
4) traditional vacuum: enzymolysis solution is put temperature 10 DEG C in vacuum centrifuge, vacuum tightness-0.01MPa, 6000r/min traditional vacuum 5min, be separated from top to bottom and obtain free oil, milk sap, hydrolyzed solution and Semen Armeniacae Amarum slag, it is 30 DEG C by the milk sap control temperature obtained, add the biological demulsifying bacteria culture fluid breakdown of emulsion 40min of milk sap quality 5%, in temperature 10 DEG C after breakdown of emulsion, vacuum tightness-0.01MPa, 2000r/min again traditional vacuum 10min obtains free oil, merge the free oil of gained, add the natural antioxidants of its quality 0.01%, namely Homogeneous phase mixing obtains Semen Armeniacae Amarum oil,
The preparation method of described biological demulsifying bacteria culture fluid is: inoculate 1 ring in 100mL broth culture by after the test tube slant actication of culture of Alcaligenes Alcaligenessp.S-XJ-1, 30 DEG C, 24h cultivated by 120r/min shaking table, then be inoculated in 2000mL seed culture medium, 30 DEG C, 36h cultivated by 110r/min shaking table, obtain seed liquor, seed liquor is inoculated in the inoculum size of volume ratio 7% in the airlift fermentor that 20L fermention medium is housed, in 35 DEG C, air flow 2L/min constant temperature open fermentation 48h, then 28 DEG C are cooled to the speed of 0.6 DEG C/h, seed liquor is continued to add inoculation with volume ratio 3% inoculum size, close tank fermentation 24h, keep tank pressure 0.01MPa, last with the ramp to 35 DEG C of 0.8 DEG C/h, namely air flow 1L/min constant temperature open fermentation 24h obtains biological demulsifying bacteria culture fluid,
Described broth culture consists of: extractum carnis 5.0g, peptone 10.0g, yeast extract paste 5.0g, NaCl5.0g, glucose 10.0g, distilled water 1L, pH value 7.0;
Described seed culture medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 1g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 2% with culture volume;
Described fermention medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 2g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 4% with culture volume;
The quality group of described natural antioxidants becomes: tea-polyphenol: grape pip procyanidin: Vc=2:1:1.
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 96.5%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.05mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 6 years; The demulsification efficiency of biological demulsifying is 98.6%.
Embodiment 4
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
1) microwave exposure goes out enzyme: Semen Armeniacae Amarum is immersed mass percent concentration and be room temperature rinsing in the sodium hydrogen carbonate solution of 0.15%, drain, put sealing and standing in container and soak 4h, then peel, decortication Semen Armeniacae Amarum is put into microwave dryer and to go out enzyme 4min in frequency 2450MHz, power 3000W, temperature 90 DEG C, bed thickness 5cm condition microwave exposure;
2) freezing crushing: the Semen Armeniacae Amarum after microwave exposure goes out enzyme is soaking at room temperature 4h in the citric acid solution of 0.3% at mass percent concentration, clear water rinsing 3 times, drain, in-25 DEG C, the freezing 5h of bed thickness 10cm, be crushed to particle diameter 0.4mm and obtain Semen Armeniacae Amarum powder;
3) enzymolysis: the pH value adding its quality 8 times in Semen Armeniacae Amarum powder is the solion of 5.5, Homogeneous phase mixing, controlling mixture temperature is 55 DEG C, adds the compound enzyme of Semen Armeniacae Amarum opaque amount 0.05% wherein, stirs, insulation, enzymolysis 60min;
Described solion is the aqueous solution containing sodium ion 33mg/L, magnesium ion 25mg/L, zine ion 25mg/L, potassium ion 23mg/L and calcium ion 14mg/L;
The quality group of described compound enzyme becomes: aspartic protease: cellulase: polygalacturonase: beta-glucanase: amylase=15:4:3:3:2;
4) traditional vacuum: enzymolysis solution is put 8000r/min traditional vacuum 8min in vacuum centrifuge, be separated from top to bottom and obtain free oil, milk sap, hydrolyzed solution and Semen Armeniacae Amarum slag, it is 50 DEG C by the milk sap control temperature obtained, add the biological demulsifying bacteria culture fluid breakdown of emulsion 60min of milk sap quality 8%, after breakdown of emulsion in 3000r/min again traditional vacuum 15min obtain free oil, merge the free oil of gained, add the Semen Vitis viniferae extract of its quality 0.03%, namely Homogeneous phase mixing obtains Semen Armeniacae Amarum oil;
The preparation method of described biological demulsifying bacteria culture fluid is: inoculate 1 ring in 100mL broth culture by after the test tube slant actication of culture of Alcaligenes Alcaligenessp.S-XJ-1, 35 DEG C, 36h cultivated by 130r/min shaking table, then be inoculated in 2000mL seed culture medium, 35 DEG C, 48h cultivated by 120r/min shaking table, obtain seed liquor, seed liquor is inoculated in the inoculum size of volume ratio 7% in the airlift fermentor that 20L fermention medium is housed, in 40 DEG C, air flow 4L/min constant temperature open fermentation 60h, then 32 DEG C are cooled to the speed of 0.8 DEG C/h, seed liquor is continued to add inoculation with volume ratio 3% inoculum size, close tank fermentation 30h, keep tank pressure 0.03MPa, last with the ramp to 40 DEG C of 1.0 DEG C/h, namely air flow 3L/min constant temperature open fermentation 30h obtains biological demulsifying bacteria culture fluid,
Described broth culture consists of: extractum carnis 5.0g, peptone 10.0g, yeast extract paste 5.0g, NaCl5.0g, glucose 10.0g, distilled water 1L, pH value 7.0;
Described seed culture medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 3g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 2% with culture volume;
Described fermention medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 5g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 4% with culture volume;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 95%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.07mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 5 years; The demulsification efficiency of biological demulsifying is 98.1%.
Embodiment 5
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
Step 1) to step 4) other technique and parameter with embodiment 2, only adopt biological demulsifying agent breakdown of emulsion;
Biological demulsifying agent addition: 0.05%;
The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=8:6:4;
The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl glycoside=8:2;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 96%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.05mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 6 years; The demulsification efficiency of biological demulsifying is 97.5%.
Embodiment 6
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
Step 1) to step 4) other technique and parameter with embodiment 3, only adopt biological demulsifying agent breakdown of emulsion;
Biological demulsifying agent addition: 0.04%;
The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=6:5:7;
The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl glycoside=7:1;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 94%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.06mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 6 years; The demulsification efficiency of biological demulsifying is 96.4%.
Embodiment 7
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
Step 1) to step 4) other technique and parameter with embodiment 4, only adopt biological demulsifying agent breakdown of emulsion;
Biological demulsifying agent addition: 0.06%;
The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=10:7:5;
The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl glycoside=9:3;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 94%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.07mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 5 years; The demulsification efficiency of biological demulsifying is 96%.
Embodiment 8
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
Step 1) to step 4) other technique and parameter with embodiment 2, only adopt biological demulsifying agent breakdown of emulsion;
Biological demulsifying agent addition: 0.05%;
The quality group of described biological demulsifying agent becomes: rhamnolipid: lipopeptid class: cell wall is in conjunction with class=8:6:4;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 93%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.07mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 5 years; The demulsification efficiency of biological demulsifying is 96%.
Embodiment 9
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
Step 1) to step 4) other technique and parameter with embodiment 4, only adopt biological demulsifying agent breakdown of emulsion;
Biological demulsifying agent addition: 0.05%;
The quality group of described biological demulsifying agent becomes: alkyl glycoside: lipopeptid class: cell wall is in conjunction with class=8:6:4;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 94%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.07mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 5 years; The demulsification efficiency of biological demulsifying is 96.6%.
Embodiment 10
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
Step 1) to step 4) other technique and parameter with embodiment 2, only adopt biological demulsifying agent breakdown of emulsion;
Biological demulsifying agent addition: 0.05%;
Described biological demulsifying agent is rhamnolipid;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 95%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.06mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 6 years; The demulsification efficiency of biological demulsifying is 97.2%.
Embodiment 11
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
Step 1) rinsing liquid and step 2) soak solution is water, and other technique and parameter are with embodiment 2;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 95%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.05mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 5 years; The demulsification efficiency of biological demulsifying is 98.1%.
Embodiment 12
An extracting method for local flavor Semen Armeniacae Amarum oil, comprises the steps:
Step 1) rinsing liquid and step 2) soak solution is water, and only adopt biological demulsifying agent breakdown of emulsion, other technique and parameter are with embodiment 2;
Biological demulsifying agent addition: 0.05%;
The quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=8:6:4;
The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl glycoside=8:2;
The extraction yield that aforesaid method prepares Semen Armeniacae Amarum oil is 94%; In Semen Armeniacae Amarum oil, the residual quantity of prussiate is 0.05mg/kg; Under room temperature, ventilation, lucifuge condition, the quality guaranteed period of Semen Armeniacae Amarum oil is 5 years; The demulsification efficiency of biological demulsifying is 96.8%.
The lipid acid composition of local flavor Semen Armeniacae Amarum oil prepared by embodiment 13 the present invention
The Semen Armeniacae Amarum oil not adding antioxidant that the Semen Armeniacae Amarum oil not adding natural antioxidants prepared with the embodiment of the present invention 2 and the employing aqueous enzymatic method of commercially available identical date manufactured are produced is for sample, and detect the lipid acid composition of Semen Armeniacae Amarum oil, detected result is as table 1.Gas-chromatography (GC) method measures.Sample esterification: take about 100mg oil sample, be placed in tool plug test tube, add the mixed solution of 1 ~ 2mL sherwood oil (30-60 DEG C) and benzene (1:1), after jolting makes lipid solubilization, add 0.4mol/LKOH-methanol solution 1 ~ 2mL, after mixing, left at room temperature 5 ~ 10min, add 12mL water again, get supernatant liquid after leaving standstill, carry out stratographic analysis.Analytical conditions for gas chromatography: quartz capillary column model: DB-nAX (3cm × 0.25mm); Column temperature: 50 DEG C keep 5min, are raised to 230 DEG C with 10 DEG C/min temperature rise rate, keep 20min; Detect mouth: FID270 DEG C; Injection port: 250 DEG C.
Table 1: the lipid acid composition of Semen Armeniacae Amarum oil
Composition (%) C 16:0 C 16:1 C 18:0 C 18:1 C 18:2 C 18:3 C 20:1 Total unsaturated fatty acids
The present invention 3.9 1.0 0.3 72.4 22.1 0.3 0 95.8
Commercially available 4.5 0.8 1 72.3 21.2 0.1 0.1 94.5
Above result shows: Semen Armeniacae Amarum oil main fatty acid composition prepared by the present invention has 7 kinds, is respectively palmitinic acid (C16:0), stearic acid (C18:0), palm monoenoic acid (C16:1), oleic acid (C18:1), linolic acid (C18:2), linolenic acid (C18:3), peanut monoenoic acid (C20:1).Wherein be mainly oleic acid and linolic acid; unsaturated fatty acid content is 95.8%; higher than commercially available Semen Armeniacae Amarum oil unsaturated fatty acid content; although otherness is little; but also fully show advance and the practicality of extraction process of the present invention, particularly illustrate the anti-oxidation protection effect to polyunsaturated fatty acid.
It should be noted that: Semen Armeniacae Amarum oil prepared by embodiment of the present invention 3-12 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.
The quality index of embodiment 14 local flavor Semen Armeniacae Amarum oil of the present invention detects
The Semen Armeniacae Amarum oil not adding antioxidant that the Semen Armeniacae Amarum oil not adding natural antioxidants prepared with the embodiment of the present invention 2 and the employing aqueous enzymatic method of commercially available identical date manufactured are produced is for sample, detect outward appearance and the physical and chemical quality indexes of Semen Armeniacae Amarum oil, detected result is as table 2.Detect establishing criteria: acid number: GB/T5530-2005; Peroxide value: GB/T5538-2005; Iodine number: GB/T5532-2008; Saponification value GB/T5534-2008; Refractive index: GB/T5527-2010; Moisture and volatile matter content: GB/T5528-2008; Transparency, smell, flavour: GB/T5525-2008; Color and luster: GB/T5492-2008.
Table 2: Semen Armeniacae Amarum oil quality index detected result
Project The present invention Commercially available
Acid number/mgKOH/g 0.45 0.67
Iodine number/gI 2/100g 100.5 88.4
Saponification value/mgKOH/g 168.2 218.5
Peroxide value/mmol/kg 0.06 0.16
Refractive index 1.4787 1.4638
Moisture and volatile matter content/% 3.2 9.6
Smell There are obvious apricot flavor, free from extraneous odour, bitter taste There are apricot flavor, free from extraneous odour, slightly hardship
Transparency Transparent, clarification Transparent, clarification
Color and luster Light yellow Faint yellow
Above result shows: the Semen Armeniacae Amarum oil prepared with commercially available employing aqueous enzymatic method, Semen Armeniacae Amarum oil acid number low (32.28%), iodine number high (13.6%), saponification value low (23.02%), peroxide value low (62.5%), refractive index high (1%), moisture content low (66.66%) that the present invention extracts, illustrate that its unsaturated fatty acid content loses less, content is high, anti-oxidant degree is high.Especially, it should be noted that moisture content is lower, demonstrate the present invention and adopt the effect of biological demulsifying better, demulsification efficiency is high, and oil-water separation is good, and recovery rate is high.Above-mentioned quality index is all significantly better than commercially available prod, and is far superior to national edible oil standard.
It should be noted that: Semen Armeniacae Amarum oil prepared by embodiment of the present invention 3-12 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.
The oxidation stability test of embodiment 15 local flavor Semen Armeniacae Amarum oil of the present invention
The Semen Armeniacae Amarum oil not adding antioxidant that the Semen Armeniacae Amarum oil not adding natural antioxidants prepared with the embodiment of the present invention 2 and the employing aqueous enzymatic method of commercially available identical date manufactured are produced is for sample, and detect Semen Armeniacae Amarum oil oxidation stability, detected result is as table 3.Detection method: adopt 743Rancimat Oxidation of Fat and Oils stabilometer, measures the inductive phase (inductionperiod) of 2 kinds of Semen Armeniacae Amarum oils 110,120,130 DEG C time respectively.Condition determination: amount of samples (30 ± 001) g; Air flow quantity 20L/h; 60mL distilled water is added in measuring cell; Reach design temperature to start to measure.
Table 3: the Semen Armeniacae Amarum oil inductive phase of detected result at different temperatures (OSI/h)
Project 110℃ 120℃ 130℃
The present invention 28.96 15.24 8.98
Commercially available 17.75 8.68 4.01
Above result shows: Semen Armeniacae Amarum oil prepared by the present invention induction time that has at different temperatures is nearly 2 times of commercially available Semen Armeniacae Amarum oil, and its oxidative stability is stronger.
It should be noted that: Semen Armeniacae Amarum oil prepared by embodiment of the present invention 3-12 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.
The V of embodiment 16 local flavor Semen Armeniacae Amarum oil of the present invention econtent and composition thereof
The Semen Armeniacae Amarum oil not adding antioxidant that the Semen Armeniacae Amarum oil not adding natural antioxidants prepared with the embodiment of the present invention 2 and the employing aqueous enzymatic method of commercially available identical date manufactured are produced, for sample, detects the V of Semen Armeniacae Amarum oil econtent and composition thereof, detected result is as table 4.Detection method: high performance liquid chromatography (HPLC) method measures.Liquid phase chromatogram condition: chromatographic column BDSC18, UV-detector, determined wavelength: 300nm, moving phase 100% methyl alcohol, flow velocity: 1mL/min, column temperature 30 DEG C, retention time 20min.
Table 4: Semen Armeniacae Amarum oil V econtent and composition (mg/100g) thereof
Project а-tocopherol 5,8-dimethyl tocol V EContent
The present invention 2.025 35.225 37.25
Commercially available 1.765 33.135 34.90
Above result shows: topmost biological activity nutritive substance V in Semen Armeniacae Amarum oil of the present invention elose low, content is higher, than commercially available similar-type products height 2.35mg/100g, illustrates that extraction process of the present invention is conducive to maximum acquisition that is anti-oxidant and biologically active substance, is conducive to improving the quality of products.
It should be noted that: Semen Armeniacae Amarum oil prepared by embodiment of the present invention 3-12 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.
The quality guaranteed period prediction experiment of embodiment 17 local flavor Semen Armeniacae Amarum oil of the present invention
The Semen Armeniacae Amarum oil of the interpolation antioxidant that the Semen Armeniacae Amarum oil of interpolation natural antioxidants prepared with the embodiment of the present invention 2 is produced with the employing aqueous enzymatic method of commercially available identical date manufactured is for sample, and do not add antioxidant in contrast with the present invention, be placed in accelerated oxidation under 65 DEG C of constant temperatures, every 20 days sampling and measuring peroxide values, and the shelf-lives of Semen Armeniacae Amarum oil under calculating normal temperature (25 DEG C) condition of storage, it the results are shown in Table 5 and table 6.
Table 5: the peroxide value (mmol/kg) of different time sections Semen Armeniacae Amarum oil
Time (d) 20 40 60 80 100
The present invention 0.08 1.08 1.65 2.06 3.25
Commercially available 2.16 10.02 14.11 18.18 20.05
Table 6: the induction time of Semen Armeniacae Amarum oil, Antioxidative Factors and quality guaranteed period prediction
Project Induction time (d) Antioxidative Factors Quality guaranteed period (d)
The present invention 137 5.48 2192 (6 years)
Commercially available 40 1.6 640 (1 year 8 months)
Contrast 25 1 400 (in January, 1)
Above result shows: commercially available Semen Armeniacae Amarum oil and the anti-oxidant value of control group rise very fast, wherein control group is only induced and is just exceeded edible standard value in 25 days, it is 1 antinutritional factor, commercially available Semen Armeniacae Amarum oil is not up to standard when 40 days on ground, and be 1.6 antinutritional factor, Semen Armeniacae Amarum oil induction time of the present invention is the longest is 137 days, be 5.48 antinutritional factor, according to the quality guaranteed period measuring and calculating of each 16 days days of induction, the quality guaranteed period of Semen Armeniacae Amarum oil of the present invention the longest is 2192 days, about 6 years; The quality guaranteed period of commercially available Semen Armeniacae Amarum oil is 640 days, about 1 year 8 months; Control group is only 400 days about 1 year.
It should be noted that: Semen Armeniacae Amarum oil prepared by embodiment of the present invention 3-12 has above-mentioned experiment effect equally, the quality guaranteed period can reach more than 5 years.

Claims (10)

1. an extracting method for local flavor Semen Armeniacae Amarum oil, is characterized in that, comprises the steps:
1) microwave exposure goes out enzyme: Semen Armeniacae Amarum is immersed water or mass percent concentration is room temperature rinsing in the sodium hydrogen carbonate solution of 0.05-0.15%, drains, put sealing and standing in container and soak 2-4h, then peel, decortication Semen Armeniacae Amarum is put into microwave dryer and to go out enzyme 2-4min in frequency 2450MHz, power 1000-3000W, temperature 80-90 DEG C, bed thickness 3-5cm condition microwave exposure;
2) freezing crushing: the Semen Armeniacae Amarum after microwave exposure goes out enzyme is soaking at room temperature 2-4h in the citric acid solution of 0.1-0.3% at water or mass percent concentration, clear water rinsing 1-3 time, drain, in-20--25 DEG C, the freezing 3-5h of bed thickness 8-10cm, be crushed to particle diameter 0.2-0.4mm and obtain Semen Armeniacae Amarum powder;
3) enzymolysis: adding its quality 6-8 pH value doubly in Semen Armeniacae Amarum powder is the solion of 3.5-5.5, Homogeneous phase mixing, and controlling mixture temperature is 45-55 DEG C, add the compound enzyme of Semen Armeniacae Amarum opaque amount 0.03-0.05% wherein, stir, insulation, enzymolysis 40-60min;
4) traditional vacuum: enzymolysis solution is put 6000-8000r/min traditional vacuum 5-8min in vacuum centrifuge, be separated from top to bottom and obtain free oil, milk sap, hydrolyzed solution and Semen Armeniacae Amarum slag, be 30-50 DEG C by the milk sap control temperature obtained, add the biological demulsifying agent of milk sap quality 0.04-0.06% or the biological demulsifying bacteria culture fluid breakdown of emulsion 40-60min of 5-8%, after breakdown of emulsion in 2000-3000r/min again traditional vacuum 10-15min obtain free oil, merge the free oil of gained, add the natural antioxidants of its quality 0.01-0.03%, namely Homogeneous phase mixing obtains Semen Armeniacae Amarum oil,
Described solion is the aqueous solution containing sodium ion 28-33mg/L, magnesium ion 20-25mg/L, zine ion 20-25mg/L, potassium ion 18-23mg/L and calcium ion 12-14mg/L;
The quality group of described compound enzyme becomes: aspartic protease: cellulase: polygalacturonase: beta-glucanase: amylase=10-15:2-4:1-3:1-3:1-2.
2. the extracting method of a kind of local flavor Semen Armeniacae Amarum oil as claimed in claim 1, is characterized in that, described microwave exposure is Batch irradiation: microwave exposure 10s, interval 20s.
3. the extracting method of a kind of local flavor Semen Armeniacae Amarum oil as claimed in claim 1, is characterized in that, described traditional vacuum condition is temperature 10-20 DEG C, vacuum tightness-0.01--0.03MPa.
4. the extracting method of a kind of local flavor Semen Armeniacae Amarum oil as claimed in claim 1, is characterized in that, described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall are in conjunction with class biological demulsifying agent 6-10:5-7:3-5 Homogeneous phase mixing in mass ratio.
5. the extracting method of a kind of local flavor Semen Armeniacae Amarum oil as claimed in claim 4, is characterized in that, the quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl glycoside=7-9:1-3.
6. the extracting method of a kind of local flavor Semen Armeniacae Amarum oil as claimed in claim 1, it is characterized in that, the preparation method of described biological demulsifying bacteria culture fluid is: inoculate 1 ring in 100mL broth culture by after the test tube slant actication of culture of Alcaligenes Alcaligenessp.S-XJ-1, 30-35 DEG C, 24-36h cultivated by 120-130r/min shaking table, then be inoculated in 2000mL seed culture medium, 30-35 DEG C, 36-48h cultivated by 110-120r/min shaking table, obtain seed liquor, seed liquor is inoculated in the inoculum size of volume ratio 7% in the airlift fermentor that 20L fermention medium is housed, in 35-40 DEG C, air flow 2-4L/min constant temperature open fermentation 48-60h, then 28-32 DEG C is cooled to the speed of 0.6-0.8 DEG C/h, seed liquor is continued to add inoculation with volume ratio 3% inoculum size, close tank fermentation 24-30h, keep tank pressure 0.01-0.03MPa, last with the ramp of 0.8-1.0 DEG C/h to 35-40 DEG C, namely air flow 1-3L/min constant temperature open fermentation 24-30h obtains biological demulsifying bacteria culture fluid.
7. the extracting method of a kind of local flavor Semen Armeniacae Amarum oil as claimed in claim 6, it is characterized in that, described seed culture medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 1-3g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 2% with culture volume; Described fermention medium consists of: NH 4nO 34.0g, K 2hPO 44.0g, KH 2pO 46.0g, MgSO 47H 2o0.2g, FeSO 47H 2o1mg, NaCl25g, CaCl 22H 2o0.8mg, ethylenediamine tetraacetic acid (EDTA) 1.3mg, Chinese herbal medicine extract 2-5g, distilled water 1L, pH value 7.0, carbon source is rapeseed oil, adds than 4% with culture volume.
8. the extracting method of a kind of local flavor Semen Armeniacae Amarum oil as claimed in claim 7, it is characterized in that, the preparation method of described Chinese herbal medicine extract, comprise the steps: to count by weight, take Radix Glycyrrhizae 15-17 part, Radix Astragali 13-16 part, Herba Epimedii 12-15 part, rhizoma zingiberis 12-15 part, root of Dahurain angelica 8-12 part, Radix Codonopsis 5-8 part; Put in the Ultrasonic Cleaners filling 0.1-0.3% sodium hydrogen carbonate solution and clean 10-15min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixture, control temperature 70-90 DEG C keeps 2-4h, then 40-50 DEG C is cooled to, be 3.5-4.5 by lactic acid adjust ph, under power 150-300W condition, carry out microwave extraction 10-15min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic-assisted extraction simultaneously; Then the prozyme adding mixture gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixture 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature keeps 3-4h to 60-78 DEG C, filters, obtains the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, obtains Chinese herbal medicine extract;
Described prozyme is dextranase, pentosanase, zytase, tannase 2-4:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio.
9. the extracting method of a kind of local flavor Semen Armeniacae Amarum oil as claimed in claim 1, it is characterized in that, the quality group of described natural antioxidants becomes: tea-polyphenol: grape pip procyanidin: Vc=2-3:1-3:1-2.
10. the local flavor Semen Armeniacae Amarum oil that extracting method as arbitrary in claim 1-9 is obtained.
CN201510740407.2A 2015-11-04 2015-11-04 Flavored bitter almond oil and extraction method thereof Pending CN105368570A (en)

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