CN105642455A

CN105642455A - Flow-controllable nozzle for automatic nucleic acid extraction apparatus and automatic nucleic acid extraction apparatus

Info

Publication number: CN105642455A
Application number: CN201610136423.5A
Authority: CN
Inventors: 车团结; 徐进章; 沈颂东; 李琳; 李亚鹏
Original assignee: Suzhou Baiyuan Gene Technology Co Ltd
Current assignee: Suzhou Baiyuan Gene Technology Co Ltd
Priority date: 2016-03-10
Filing date: 2016-03-10
Publication date: 2016-06-08
Anticipated expiration: 2036-03-10
Also published as: CN105642455B

Abstract

The invention discloses a flow-controllable nozzle for an automatic nucleic acid extraction apparatus and the automatic nucleic acid extraction apparatus. The nozzle comprises a fluid pipeline and a first piezoelectric actuator, wherein one end of the fluid pipeline is connected to a fluid supply pipeline and the other end of the fluid pipeline is connected to the first piezoelectric actuator; and a hole allowing fluid to pass through is formed in a middle part of the first piezoelectric actuator, fluid flowing into the fluid pipeline from the fluid supply pipeline flows out from the hole, and the shape of the hole in the middle part of the first piezoelectric actuator can be changed under application of voltage control. The flow-controllable nozzle for the automatic nucleic acid extraction apparatus can achieve controllable regulation on the fluid flowing out of the nozzle and prevents the situations of fluid leakage and fluid dropping.

Description

Flow controllable nozzles and nucleic acid automatic extracting instrument for nucleic acid automatic extracting instrument

Technical field

The present invention relates to nucleic acid-extracting apparatus field, be specifically related to a kind of flow controllable nozzles for nucleic acid automatic extracting instrument and nucleic acid automatic extracting instrument.

Background technology

Instrument for extracting nucleic acid, is the instrument extracting work applying supporting nucleic acid extracting reagent to be automatically performed sample nucleic acid, and instrument for extracting nucleic acid is divided into two classes: a class is large-scale automatization, is commonly referred to as automated fluid work station; Another kind of is small-sized automatic instrument for extracting nucleic acid, utilizes packaged matched reagent to be automatically performed extraction purification process. Large-scale automated fluid work station is because equipment cost is high, and operating cost is high, is suitable for once extracting several thousand one species specimen, so what be really applied is fewer; And the instrument of small automatic, because instrument and equipment and operating cost are low, easy to operate, obtain increasing application.

Such as, disclosed in gloomy Shou Hong etc. propose method for extracting nucleic acid and nucleic acid-extracting apparatus (CN101886037A) a kind of nucleic acid-extracting apparatus, belong to the instrument of small automatic, utilize this nucleic acid-extracting apparatus to process the sample solution containing nucleic acid, there is efficient, easy, quick, good automatization's adaptability and the advantage of good repeatability. Cleaning mixture is dispensed into multiple in the nucleic acid extraction column of arrangement by the first dispensing mouth by this device, is dispensed into by recovered liquid multiple in the nucleic acid extraction column of arrangement by the second dispensing mouth. But, above-mentioned dispensing mouth is when dispensing liquid, and dispensing mouth cannot be controlled regulating according to attribute and liquid outflow amounts etc. such as the toughness of liquid, causes there is the situation such as leakage, dropping liquid, can pollute surrounding enviroment.

Summary of the invention

Therefore, the embodiment of the present invention to solve the technical problem that and to be in that dispensing mouth of the prior art cannot automatic control flow.

For this, a kind of flow controllable nozzles for nucleic acid automatic extracting instrument of the embodiment of the present invention, including:

Fluid line, its one end is connected with pipeline for fluids, and its other end and the first piezo-activator connect;

First piezo-activator, has the hole passed through for fluid in the middle part of it, the fluid flowing into described fluid line from pipeline for fluids flows out from described hole, and the shape in the hole in the middle part of the first piezo-activator can be changed under applying Control of Voltage.

Preferably, described hole is generally circular in shape or oval.

Preferably, described first piezo-activator be shaped as disc-shaped.

Preferably, also include:

At least one second piezo-activator, is positioned on the tube wall of described fluid line, for changing the diameter of described fluid line under applying Control of Voltage.

Preferably, the number of described second piezo-activator is 3-6.

Preferably, described second piezo-activator is uniformly distributed on the tube wall being positioned at described fluid line.

Preferably, described fluid line is cylindrical or conical; When fluid line is cone, the port of the one end of the described fluid line being connected with pipeline for fluids is more than the port of the other end of the described fluid line being connected with the first piezo-activator.

Preferably, the cone angle of described cone is 30 degree of-45 degree.

A kind of nucleic acid automatic extracting instrument of the embodiment of the present invention, including above-mentioned nozzle.

The technical scheme of the embodiment of the present invention, has the advantage that

The flow controllable nozzles for nucleic acid automatic extracting instrument that the embodiment of the present invention provides, by arranging the first piezo-activator, make size and the reconfigurable joint in the hole of outflow fluid, it is achieved thereby that the controllable adjustment of the flow of mass flowing nozzle, namely the flow of automatic control and adjustment nozzle can be carried out according to the demand of the attributes such as the toughness of fluid and actual flow output, it is prevented that the appearance of the situation such as leakage, dropping liquid.

Accompanying drawing explanation

In order to be illustrated more clearly that the technical scheme in the specific embodiment of the invention, below the accompanying drawing used required during detailed description of the invention is described is briefly described, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.

Fig. 1 is the axial sectional view of the flow controllable nozzles for nucleic acid automatic extracting instrument of one concrete example of the present invention;

Fig. 2 is the schematic shapes of the first piezo-activator of one concrete example of the present invention;

Fig. 3 is the top view of the flow controllable nozzles for nucleic acid automatic extracting instrument of another concrete example of the present invention;

Fig. 4 is the top view of the flow controllable nozzles for nucleic acid automatic extracting instrument of another concrete example of the present invention.

Accompanying drawing labelling: 1-fluid line, 2-the first piezo-activator, 3-the second piezo-activator, 21-hole, f1, f2, f3-deformation direction.

Detailed description of the invention

Below in conjunction with accompanying drawing, technical scheme is clearly and completely described, it is clear that described embodiment is a part of embodiment of the present invention, rather than whole embodiments. Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.

In describing the invention, it should be noted that, term " on ", D score, the orientation of the instruction such as "left", "right" or position relationship be based on orientation shown in the drawings or position relationship, it is for only for ease of the description present invention and simplifies description, rather than instruction or hint indication device or element must have specific orientation, with specific azimuth configuration and operation, be therefore not considered as limiting the invention. Term " first ", " second " etc. only for descriptive purposes, and it is not intended that instruction or hint relative importance. Unless otherwise clearly defined and limited, term " installation ", " being connected ", " connection " should be interpreted broadly, for instance, it is possible to it is fixing connection, it is also possible to be removably connect, or connect integratedly; Can be mechanically connected, it is also possible to be electrical connection; Can be joined directly together, it is also possible to be indirectly connected to by intermediary, it is also possible to be the connection of two element internals, it is possible to be wireless connections, it is also possible to be wired connection. For the ordinary skill in the art, it is possible to concrete condition understands above-mentioned term concrete meaning in the present invention.

As long as just can be combined with each other additionally, technical characteristic involved in invention described below difference embodiment does not constitute conflict each other.

Embodiment 1

Present embodiments provide a kind of flow controllable nozzles for nucleic acid automatic extracting instrument, as it is shown in figure 1, include: fluid line 1 and the first piezo-activator 2. One end of fluid line 1 is connected with pipeline for fluids, and the other end and first piezo-activator 2 of fluid line 1 connect. The middle part of the first piezo-activator 2 has the hole 21 passed through for fluid, the flow direction of fluid is direction f1 as shown in Figure 1, flowing out from hole 21 from the fluid of pipeline for fluids incoming fluid pipeline 1, the shape in the hole 21 in the middle part of the first piezo-activator 2 can be changed under applying Control of Voltage. First piezo-activator 2 can adopt piezoelectric actuator.

The above-mentioned flow controllable nozzles for nucleic acid automatic extracting instrument, by arranging the first piezo-activator, make size and the reconfigurable joint in the hole of outflow fluid, it is achieved thereby that the controllable adjustment of the flow of mass flowing nozzle, namely the flow of automatic control and adjustment nozzle can be carried out according to the demand of the attributes such as the toughness of fluid and actual flow output, it is prevented that the appearance of the situation such as leakage, dropping liquid.

Preferably, hole 21 is generally circular in shape or oval. Preferably, the first piezo-activator 2 be shaped as disc-shaped. As shown in Figure 2, first piezo-activator 2 is disc-shaped, hole 21 generally circular in shape, after the first piezo-activator 2 is applied in voltage, first piezo-activator 2 produces deformation according to deformation direction f2, direction f2 is the diametric(al) along the first piezo-activator 2, such that it is able to the diameter of adjustment hole 21.

Preferably, as it is shown on figure 3, the flow controllable nozzles for nucleic acid automatic extracting instrument also includes: at least one second piezo-activator 3. Second piezo-activator 3 is positioned on the tube wall of fluid line 1, for changing the diameter of fluid line 1 under applying Control of Voltage. The deformation direction f3 of the second piezo-activator 3 is the radial direction along fluid line 1, makes the diameter of fluid line 1 become big or diminish.

Preferably, the number of the second piezo-activator 3 is 3-6. Second piezo-activator 3 is uniformly distributed on the tube wall being positioned at fluid line 1. Second piezo-activator 3 can also be distributed entirely around fluid line 1. By changing the diameter of fluid line 1, regulate the pressure of nozzle such that it is able to control the flow of mass flowing nozzle further, it is achieved automatically control the purpose of nozzle flow.

Preferably, fluid line 1 is cylindrical or conical; When fluid line 1 is cone, the port of the one end of the fluid line 1 being connected with pipeline for fluids is more than the port of the other end of the fluid line 1 being connected with the first piezo-activator 2.

Preferably, the cone angle of cone is 30 degree of-45 degree.

Embodiment 2

The present embodiment provides a kind of nucleic acid automatic extracting instrument, including the nozzle of embodiment 1.

Obviously, above-described embodiment is only for clearly demonstrating example, and is not the restriction to embodiment. For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description. Here without also cannot all of embodiment be given exhaustive. And the apparent change thus extended out or variation are still among the protection domain of the invention.

Claims

1. the flow controllable nozzles for nucleic acid automatic extracting instrument, it is characterised in that including:

Fluid line (1), its one end is connected with pipeline for fluids, and its other end and the first piezo-activator (2) connect;

First piezo-activator (2), there is in the middle part of it hole (21) passed through for fluid, the fluid flowing into described fluid line (1) from pipeline for fluids flows out from described hole (21), and the shape in the hole (21) at the first piezo-activator (2) middle part can be changed under applying Control of Voltage.

2. nozzle according to claim 1, it is characterised in that described hole (21) generally circular in shape or oval.

3. nozzle according to claim 1 and 2, it is characterised in that described first piezo-activator (2) be shaped as disc-shaped.

4. the nozzle according to any one of claim 1-3, it is characterised in that also include:

At least one second piezo-activator (3), is positioned on the tube wall of described fluid line (1), for changing the diameter of described fluid line (1) under applying Control of Voltage.

5. nozzle according to claim 4, it is characterised in that the number of described second piezo-activator (3) is 3-6.

6. the nozzle according to claim 4 or 5, it is characterised in that described second piezo-activator (3) is uniformly distributed on the tube wall being positioned at described fluid line (1).

7. the nozzle according to any one of claim 1-6, it is characterised in that described fluid line (1) is for cylindrical or conical; When fluid line (1) is cone, the port of the one end of the described fluid line (1) being connected with pipeline for fluids is more than the port of the other end of the described fluid line (1) being connected with the first piezo-activator (2).

8. nozzle according to claim 7, it is characterised in that the cone angle of described cone is 30 degree of-45 degree.

9. a nucleic acid automatic extracting instrument, it is characterised in that include the nozzle as described in any one of claim 1-8.