CN1056314A

CN1056314A - Useful bacterial strain of one strain and application thereof

Info

Publication number: CN1056314A
Application number: CN 90102847
Authority: CN
Inventors: 张李阶
Original assignee: Individual
Current assignee: Individual
Priority date: 1990-05-03
Filing date: 1990-05-03
Publication date: 1991-11-20
Anticipated expiration: 2005-05-03
Also published as: CN1029857C

Abstract

A kind of multi-functional Huaxing Humber 1 (BF839) bacterial strain that screening and separating goes out from well-developed baby or low-aged animal intestinal microflora, utilize fermentation technique to make viable bacteria system preparation, can increase upgrowth and development of children, control acute chronic enteritis, flora imbalance, upper respiratory tract infection and neurosis etc. all there are better curative effect, particularly remarkable to infantile diarrhea, malnutritive curative effect especially.Can increase livestock and poultry, the weight of animals, egg productivity and strengthen disease resistance as fodder additives, also can be used for preparing makeup, protective foods and beverage etc.

Description

Useful bacterial strain of one strain and application thereof

The invention belongs to technical field of bioengineering, particularly relate to a kind of Huaxing Humber 1 (BF893) bacterial strain and application thereof that utilizes microbial fermentation technology to cultivate.

In recent years, American-European advanced country is engaged in the scholar of pathogeny microorganism, many people turn to the research to useful normal microorganism, adopt biotechnology to solve the problem that aspects such as agricultural, livestock industry, body-care, disease preventing and treating (comprise and capture the obstinate fort of cancer) and prolongs life exist.Through international online retrieval, the antitumor action document (J55092322,1978) that relates to bifidus bacillus is arranged, but do not see the report of this class strains separation of relevant Huaxing Humber 1 (BF839), cultivation and application.

The microecosystem of human body and immunity, metabolism, nutrition are relevant, decline along with this system of increase of year order is residual gradually and wear out, so the easy weak and easy cancer stricken of the people more than 60 years old less than the people of 60 years old strong shape is then denied.Purpose of the present invention is intended to isolate the probiotics that some plays an important role the microecosystem normal physiological bacterium in the low age human or animal body, be transplanted in the human or animal body at advanced age, make its recovery and set up the higher new microecosystem of biomass, improve field planting durability factor level, reach and recover or part is recovered the states such as nutrition, metabolism and immunity that low age is had period.

The present invention implements by following step: screen, isolate multi-functional Huaxing Humber 1 (BF839) bacterial strain from well-developed baby or low-aged animal intestinal microflora, utilize microbial fermentation technology to make active bacteria formulation, be used to cure multiple disease, preparation protective foods, beverage, drink additive and makeup etc.

Huaxing Humber 1 (BF839) is one of normal physiological anaerobic bacterium of human body, identify through Chinese science institute of microbiology and Inst. of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine (, all tentatively be a kind in the bacteroides fragilis group (BacteroidesFragilis).Limit because of domestic equipment and condition, ultrastructure and some feature thereof of Huaxing Humber 1 (BF839) are not made the full conclusion in garden as yet.

Through nearly 10 years studies show that, this bacterium has following function: (1) improves body immunity, increases the immune t-cell activity; (2) help the absorption of human body digest food; (3) some toxicant in the degraded intestines; (4) get rid of alien bacteria and settle down, adjust intestinal flora and distribute at enteron aisle; (5) synthetic multivitamin and protein in enteric cavity; (6) anticancer growth etc.

Can increase upgrowth and development of children by hundreds of routine clinical verification Huaxing Humber 1s (BF839) preparation, control acute chronic enteritis, flora imbalance, upper respiratory tract infection and neurosis etc. all there are better curative effect, particularly remarkable to infantile diarrhea, malnutritive curative effect especially.Can increase poultry, the weight of animals, egg productivity and strengthen disease resistance as fodder additives, also can be used for preparing makeup, protective foods and beverage etc.

Huaxing Humber 1 (BF839) is to get from well-developed baby intestinal flora, puts in the anaerobically fermenting substratum of autogamy fermentation and makes active bacteria formulation.Anaerobically fermenting substratum composition: peptone 0.5～25%, glucose 0.2～0.5%, halfcystine 0.01～0.5%, Nac10.2～0.5%, Na ₂HPO ₄0.1～0.3% sodium thioglycollate 0.02～0.1%, beef liver or pork liver immersion liquid 30～100% beef infusion broths 30～100%, yeast extract paste 0.1～0.8%.32～40 ℃ of ferment at constant temperature were cultivated 12～72 hours, and 3500 rev/mins of centrifugal supernatant liquors that go are surveyed bacterium and used.

Example one

Huaxing Humber 1 (BF839) is inoculated in the 10 liter anaerobically fermenting substratum, cultivated 24 hours for 37 ℃.Recording every milliliter of viable count is 2084 * 10 ⁶=10 ^8.2(press log10 ⁿ/ ml calculates).3000～4000 rev/mins of centrifugal supernatant liquors that go, washing, diluted for use.

Example two

Huaxing Humber 1 (BF839) is inoculated in the 3 liter anaerobically fermenting substratum 37 ℃ cultivated 30 hours, recording every milliliter of viable count is 1132 * 10 ⁷=10 ^9.1(press log10 ⁿCalculate).3000～4000 rev/mins of centrifugal supernatant liquors that go, washing, diluted for use.

Example three

Huaxing Humber 1 (BF839) is inoculated in the 10 liter anaerobically fermenting substratum, cultivated 24 hours for 37 ℃, recording every milliliter of viable count is 588 * 10 ⁸=10 ^9.8(press log10 ⁿCalculate).3000～4000 rev/mins of centrifugal supernatant liquors that go, washing, diluted for use.

Example four

BF839 living bacterial liquid 10ml(viable count 4,000,000,000/ml) of early 10 minutes after the meal clothes was observed 11.5 months altogether next day of boarding at the nursery children in 3～6 years old for the kindergarten, and the result shows and takes height and the body weight (table 1,2) that the Bf839 living bacterial liquid has increased children significantly.

Table 1, child take body weight change result behind the BF839

Continue Time of Administration (moon) n ₁(test group) n ₂(control group) T value P value

2 64 54 2.50 ＜0.05

5 53 46 2.97 ＜0.01

7.5 59 56 3.58 ＜0.01

236 patients such as youngster's diarrhoea, amebic dysentery, depressive psychosis, bacillary dysentery, constipation every day early, service once the BF839 living bacterial liquid, each 20ml(viable count 4,000,000,000/ml), the efficient 98.3%(table 4 that reaches after the supper).

Table 4, take the curative effect of BF839 to intestinal tract disease

The example number each one take a bottle number (100nl/ bottle) rehabilitation take a turn for the better invalid

Chronic intestinal inflammations 81 1～10 746 1

Acute enteritis 17 1～3 17 2

Bowel dysfunction 22 1～6 164

General maldigestion 32 1～4 302

Liver property maldigestion 8 4～10 53

Senile maldigestion 6 3～10 6

Infantile diarrhea 58 1～3 58

Amebic dysentery 141

Depressive psychosis 33

Bacillary dysentery 44

Constipation 44

Sum 236 21,418 4

Percentage 100% 90.7% 7.6% 1.7%

Table 2, child take height result of variations behind the BF839

6 53 46 1.78 ＞0.05

11.5 44 46 2.90 ＜0.01

Example five

BF839 living bacterial liquid 10ml(viable count 4,000,000,000/ml) of early 10 minutes after the meal clothes next day of boarding at the nursery children in 3～6 years old for the kindergarten, blood sampling is measured the T cell with esterase staining after 11 months, and the result shows and takes the immunologic function (table 3) that the BF839 living bacterial liquid has significantly increased children.

Table 3, T raji cell assay Raji result

n ₁(test group) ₂(control group) T value P value

Group experiment 45 38 4.18＜0.01

Pair test 30 30 3.25＜0.01

Example six

Acute enteritis, cureless chronic intestinal inflammations (part is made a definite diagnosis through enteroscopy), bowel dysfunction, general that the standard routinely of giving is made a definite diagnosis, liver property and senile disappearing are estimated bad, baby

Example seven

BF839 living bacterial liquid 10ml(viable count 2,000,000,000/ml) of early 10 minutes after the meal clothes was observed 11.5 months altogether next day of boarding at the nursery children in 3～6 years old for the kindergarten, and the result shows that the BF839 living bacterial liquid has the effect (table 5) of prevention youngster respiratory tract disease.

Table 5, clothes BF839 are to the prophylactic effect of children's upper respiratory tract sense

11.5 63 55 3.12 ＜0.01

Example eight

With 120 of the poulty house laying hens that makes on the same day of the same race, with under the living condition, use forage feed of the same race at this, stochastic sampling is divided into each 60 of test group and control groups.Test group every day admixes in the feed feeding by the amount of every chicken clothes bacterium several 2,000,000,000 with the BF839 living bacterial liquid and suffers all, continue after 31 days trial periods and the drug withdrawal to observe 21 days, altogether in 52 days 60 laying hens of test group increase the 17.43Kg(table 6 of laying eggs than 60 laying hens of control group).

Table 6, BF839 increase the egg productivity of chicken

Gross weight (Kg) weight (the Kg)/sky of on average laying eggs of laying eggs, the average egg number (only) of the sum (only) of laying eggs/sky

Test group 2,379 45.75 143.09 2.7

Control group 2,124 40.85 125.56 2.42

Claims

1, a kind of Huaxing Humber 1 bacterial strain (BF839), it is characterized in that this bacterium is that screening from well-developed baby or low-aged animal intestinal microflora, separation are provided multi-functional: improve body immunity, increase the immune t-cell activity, help the absorption of human body digest food, some toxicant in the degraded intestines is got rid of alien bacteria and is settled down at enteron aisle, adjusts intestinal flora and distributes, synthetic multivitamin and protein in enteric cavity, anticancer growth etc.This bacterium being inoculated in the anaerobically fermenting substratum of autogamy fermentation culture makes active bacteria formulation and can increase upgrowth and development of children, control acute chronic enteritis flora imbalance, upper respiratory tract infection and neurosis etc. all there are better curative effect, particularly remarkable to infantile diarrhea, malnutritive curative effect especially.Can increase livestock and poultry, the weight of animals, egg productivity and strengthen disease resistance as fodder additives, also can be used for preparing makeup, protective foods and beverage etc.

2,, it is characterized in that the anaerobically fermenting substratum that is inoculated in autogamy is made up of following compositions: peptone 0.5～25%, glucose 0.2～0.5%, halfcystine 0.01～0.5%, N by the described the sort of Huaxing Humber 1 bacterial strain of claim 1 (BF839) _aCl0.2～0.5%, N _aHP-O ₄0.1～0.3%, sodium thioglycollate 0.02～0.1%, beef liver or pork liver immersion liquid 30～100%, beef infusion broth 30～100%, yeast extract paste 0.1～0.8%.

3, by the described the sort of Huaxing Humber 1 bacterial strain of claim 1 (BF839), it is characterized in that this bacterium is inoculated in the anaerobically fermenting of autogamy, substratum fermentation culture 12～72 hours, thermostat temperature is 32～40 ℃.