CN105628853B - A kind of quality determining method of cymbopogon distans medicinal material - Google Patents
A kind of quality determining method of cymbopogon distans medicinal material Download PDFInfo
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- CN105628853B CN105628853B CN201510972088.8A CN201510972088A CN105628853B CN 105628853 B CN105628853 B CN 105628853B CN 201510972088 A CN201510972088 A CN 201510972088A CN 105628853 B CN105628853 B CN 105628853B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a kind of quality determining method of cymbopogon distans medicinal material, the true and false or quality of medicinal material is distinguished as the standard of comparison using the content of Kaempferol in cymbopogon distans medicinal material, scopoletin, three kinds of compositions of vanillic acid.The inventive method is easy to operate, chromatogram clear spot, separating effect are preferable, methodological study can show that its precision and repeatability are good, the high performance thin layer chromatography with 3 kinds of principle active components in cymbopogon distans set up, can provide certain reference information for the cold quality evaluation for breathing heavily ancestral's handkerchief particle of dimension medicine, also be trembled with fear for further investigation and breathe heavily ancestral's handkerchief particle effective substance and its quality control provides reference.
Description
Technical field
The present invention relates to the quality evaluation side of the qualitative and quantitative discrimination method, more particularly to cymbopogon distans medicinal material of medicinal material
Method.
Background technology
Ancestral's handkerchief particle is breathed heavily dimension medicine is cold, and cymbopogon distans are that, as wherein main composition medicinal material simply, have in terms of pharmacological action
Have relieving asthma, the effect such as antibechic, antibacterial.3rd, cymbopogon distans have homonym phenomenon, and more obscure always, to what is obtained in pharmaceutical factory
It is cold to breathe heavily crude drug " cymbopogon distans " progress raw medicinal herbs discriminating in ancestral's handkerchief particle, differentiated by Plant Taxonomy, it is cold to breathe heavily in ancestral's handkerchief particle
The cymbopogon distans used are Euphorbiaceae Euphorbia annual herb plant to the leaf root of Beijing euphorbia（Euphorbia sororia A）A germplasm
Amount is evaluated.
Cymbopogon distans are distributed mainly on the areas such as the Central Asia and Xinjiang of China and field with herb and fruit medicine, are Uygur medicines
Medicinal herbs most in use are learned, Uygur is entitled " Su Zhafu ", occupies critical positions in Uygur's medical science, study of pharmacy.The medicinal material has weight
The medical value wanted, as a kind of conventional medicament with long medication history, major function be inducing diuresis for removing edema, step-down cephalocathartic,
Relieving asthma, antibechic, antibacterial, rush down under miscellaneous worm.Swollen and ache for constipation, frequent micturition, Hypertension Complicated Headache, hepatic sclerosis, oedema and scabies
Deng.There are only 1997 necessarily recorded to " to the leaf root of Beijing euphorbia " now《Chinese Plants will》44th（3）Volume page 113, is simply described
Its plant shape and feature, are not discussed in detail, so research has more bases and Clinical significance of MG.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to there is provided one kind is easy to operate, stability is strong, precision
The quality determining method of high cymbopogon distans medicinal material.
The purpose of the present invention is implemented by the following technical programs：
A kind of quality determining method of cymbopogon distans medicinal material, with Kaempferol in cymbopogon distans medicinal material, scopoletin, three kinds of vanillic acid
The content of composition distinguishes the true and false or quality of medicinal material as the standard of comparison.
Cymbopogon distans medicinal material is qualitatively detected, determines that its is true and false：
With Kaempferol, scopoletin, vanillic acid composition, extract solution mistake in ethyl acetate and 25% hydrochloric acid extraction cymbopogon distans medicinal material
After filter, it is evaporated, methanol dissolving is used as need testing solution；
The medicinal material sample spot of Kaempferol, scopoletin mark product and extraction is in same silica G F254On lamellae, solvent expansion,
Inspected under 365nm uviol lamps, untested medicinal material shows same color with standard items in same position；
Vanilla acidity scale product and the medicinal material sample spot of extraction are in same silica G F254On lamellae, solvent expansion, 254nm is purple
Inspected under outer lamp, untested medicinal material shows same color with standard items in same position.
By preferred solvent, the solvent described in Kaempferol, scopoletin is toluene-ethyl acetate-formic acid, its volume ratio
For 8:3:0.7；When vanillic acid deploys, deployed with toluene-ethyl acetate-methyl alcohol-formic acid solvent, its volume ratio is 7:3:1:
0.6
Specifically quantitative detection method step is as follows：
1）It is prepared by need testing solution
Cymbopogon distans medicinal powder 5.001g is taken, ethyl acetate 50ml is added, after 25% hydrochloric acid 2.5ml is fully mixed, ultrasound
60min, filtering is transferred on evaporating dish placement water-bath and is evaporated, adds 5ml methanol and be completely dissolved, be used as need testing solution
5ml；
2）It is prepared by reference substance solution
Precision weighs Kaempferol standard items 10.58mg, scopoletin standard items 1.12mg and vanillic acid standard items 10.54mg,
Put in 10ml volumetric flasks, dissolved, Kaempferol reference substance 1.058mg/ml, scopoletin reference substance 0.112mg/ with methanol constant volume respectively
Ml, vanillic acid reference substance 1.054mg/ml, are used as reference substance solution；
3) cymbopogon distans thin-layer qualitative differentiates
The μ l of scopoletin reference substance 2, the μ l of Kaempferol reference substance 1 are drawn, the μ l of test sample 4 are put in same silica G F respectively254Thin layer
On plate, solvent expansion is taken out, dried, and is inspected under the rearmounted 365nm uviol lamps of developing the color, in test sample chromatogram, with reference substance
On the corresponding position of chromatogram, show the spot of same color；
The μ l of vanillic acid reference substance 4 are drawn, the μ l points of test sample 4 are in same silica G F254On lamellae, solvent expansion takes
Go out, dry, inspected under the rearmounted 254nm uviol lamps that develop the color, on position corresponding with reference substance chromatogram, show the spot of same color
Point；
4）Cymbopogon distans thin layer scanning is quantified
The μ l of scopoletin reference substance 2, Kaempferol reference substance 1 μ l are drawn, 10 crowdes of μ l of medicinal material test sample 4 are put in same silica gel respectively
GF254On lamellae, solvent expansion is taken out, dried, alchlor-ethanol solution using 1% is uniformly sprayed on as developer
On the lamellae dried, dry, be scanned according to chromatography again, wavelength is 330nm, measure test sample trap integrated value
With reference substance trap, calculate, produce the content of Kaempferol, scopoletin；
The μ l of vanillic acid reference substance 4,10 crowdes of μ l of medicinal material test sample 4 is drawn to put respectively in same silica G F254On lamellae, exhibition
Open, take out, dry, alchlor-ethanol solution using 1% is uniformly sprayed on the lamellae dried, dried in the air again as developer
It is dry, it is scanned according to chromatography, wavelength is 300nm, measurement test sample trap integrated value and reference substance trap are calculated,
Produce the content of vanillic acid.
It is preferred that, described step 2）In, during dissolving, solution is placed in ultrasonic wave and handles 60min.
It is preferred that, described step 3）、4）In, when Kaempferol, scopoletin expansion, described solvent is toluene-acetic acid
Ethyl ester-formic acid, its volume ratio is 8:3:0.7；When vanillic acid is opened, deployed with toluene-ethyl acetate-methyl alcohol-formic acid solvent, its
Volume ratio is 7:3:1:0.6.
It is preferred that, thin-layer developing is under the conditions of 21 ~ 25 DEG C of room temperature, 40 ~ 43RH of air humidity.
The present invention is extracted into point-score using hydrochloric acid-ethyl acetate, and extractability is strong.
Beneficial effect of the present invention：
The present invention provides a kind of Uygur's medicinal herbs most in use -- and the quality evaluating method of cymbopogon distans medicinal material, it completes cymbopogon distans
Thin-layer chromatography Qualitive test and assay, wherein assay be use tlc-scanning determination Kaempferol, henbane
The content of booth and vanillic acid.Bright through methodology proof list, this method precision, reappearance, stability and average recovery are good
It is good, show that this method meets the technical requirements of assay.
The inventive method is easy to operate, chromatogram clear spot, separating effect preferable, and methodological study can show that its is accurate
Degree and repeatability are good, the high performance thin layer chromatography with 3 kinds of principle active components in cymbopogon distans of foundation, can breathe heavily Zu Pa for dimension medicine is cold
The quality evaluation of particle provides certain reference information.Also the research of ancestral's handkerchief particle effective substance and its species are breathed heavily to be deeply cold
New drug development provide reference value.
Brief description of the drawings
Fig. 1 is Kaempferol（A）, scopoletin（B）Mixed mark and 10 batches of samples thin-layer developing figure under the conditions of ultraviolet 365nm；
Fig. 2 is scopoletin mark product（B）, Kaempferol mark product（A）With 10 batches of samples under the conditions of ultraviolet 365nm thin-layer developing figure；
Fig. 3 is vanilla acidity scale product（C）With 10 batches of samples under the conditions of ultraviolet 254nm thin-layer developing figure, C be vanilla acidity scale product；
Fig. 4 is Kaempferol（A）, scopoletin（B）Mixed mark and 10 batches of samples thin layer quantitative scanning figure under the conditions of ultraviolet 330nm；
Fig. 5 is vanillic acid（C）With 10 batches of samples under the conditions of ultraviolet 300nm thin layer quantitative scanning figure.
Embodiment
The preferred embodiments of the present invention are illustrated below, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
1. instrument and reagent
1.1 instruments
CAMAG TLC SCANNER3 types thin-layer chromatogram scanners (Switzerland)；The semi-automatic point sample instruments of CAMAG LINOMAT5 are (auspicious
Scholar)；CAMAG REPROSTAR3 (Switzerland)；Silica G F254High Performance Thin plate（Anhui Liangchen Silicon Material Co., Ltd.）；XS-
105 type electronic balances（Switzerland Mei Le-support benefit company）；The a ten thousandth electronic balance of XS105 types ten（Switzerland plum Teller-Tuo Li
Company）；KQ-200KDE type ultrasonic cleaning machines（Kunshan Ultrasonic Instruments Co., Ltd.）；HH-S4Digital display thermostat water bath（Jintan
Medical Instruments factory of city）
1.2 reagents
Cymbopogon distans medicinal material：（Xinjiang Qi Kanghabowei medicines limited company, lot number：090202-1、120916-3、
130816-2、130916-1、130916-1-113、140305-2、140227-1）, black Uygur medicine hospital of city, Hetian City people doctor
Institute and field individual Wei Yao pharmacies.
It is pure that remaining reagent is analysis.
2. method
2.1 cymbopogon distans thin-layer chromatography Qualitive tests
2.1.1 the preparation of need testing solution：Precision weighs each batch medicinal material 5.001g of the above, is respectively placed in 100ml and jumps a queue
In conical flask, plus ethyl acetate 50ml, 25% hydrochloric acid 2.5ml, ultrasonically treated 60min is mixed, filtration, filtrate is evaporated, and residue adds
5ml methanol makes dissolving, produces need testing solution（1、2、3、4、5、6、7、8、9、10）.
2.1.2 the preparation of reference substance solution：Precision weighs Kaempferol reference substance in right amount, plus methanol is made every 1ml and contained
1.058mg solution, is used as reference substance solution A；Precision weighs scopoletin reference substance in right amount, plus methanol is made every 1ml and contained
0.112mg solution, is used as reference substance solution B；Precision weighs vanillic acid reference substance in right amount, plus methanol is made every 1ml and contained
1.054mg solution, is used as reference substance solution C.
2.1.3 thin-layer chromatography condition：Draw the μ l of scopoletin reference substance 2, the μ l of Kaempferol reference substance 1, the μ l of test sample 4 difference
Point is on same silica GF254 lamellae, using volume ratio as 8:3:0.7 toluene-ethyl acetate-formic acid is solvent, is deployed,
Take out, dry, inspected under the rearmounted 365nm uviol lamps that develop the color；In test sample chromatogram, in position corresponding with reference substance chromatogram
On, show the spot of same color.The μ l of vanillic acid reference substance 4 are drawn, the μ l points of test sample 4 are in same silica G F254On lamellae, with
Volume ratio is 7:3:1:0.6 toluene-ethyl acetate-methyl alcohol-formic acid is solvent, is deployed, and takes out, dries, and is developed the color rearmounted
Inspected under 254nm uviol lamps；On position corresponding with reference substance chromatogram, show the spot of same color.
2.2 cymbopogon distans TLCSs are quantified
According to thin-layered chromatography experiment, the μ l of pipette samples solution 4, the μ l of reference substance solution A 1, the μ l of reference substance B solution 2 are put in same
One silica G F254On lamellae, using volume ratio as 8:3:0.7 toluene-ethyl acetate-formic acid is solvent, is deployed, and takes out, dries in the air
Dry, spray is developed the color with 1% alchlor-ethanol solution, is dried, is scanned according to chromatography, wavelength is 330nm, and measurement test sample is inhaled
Receipts degree integrated value and reference substance trap, calculate, produce the content of Kaempferol in cymbopogon distans, scopoletin.Vanillic acid assay:
The μ l solution of pipette samples 4, the μ l of reference substance C solution 4 are put in same silica G F254On lamellae, using volume ratio as 7:3:1:0.6
Toluene-ethyl acetate-methyl alcohol-formic acid is solvent, and consistent with above operating method, scanning wavelength is 300nm, measures test sample
Trap integrated value and reference substance trap, calculate, produce the content of vanillic acid in cymbopogon distans.
3. result
3.1 cymbopogon distans thin-layer chromatography Qualitive tests
The standard liquid prepared and 10 batches of need testing solutions are deployed by above-mentioned thin-layer chromatography condition point sample and ultraviolet
Lamp（254nm）Lower inspection is known, and obtains standard liquid and cymbopogon distans extract thin-layer chromatogram, such as Fig. 1.
3.2 cymbopogon distans tlc scanning figures
The mixed mark solution prepared and 10 batches of need testing solutions are deployed by above-mentioned thin-layer chromatography condition point sample and ultraviolet
Lamp（365nm）With（254nm）Lower inspection is known, and obtains cymbopogon distans thin-layer chromatogram, such as Fig. 1 ~ 3.By the mixed mark solution prepared and 10
Criticize need testing solution and press above-mentioned thin-layer chromatography condition point sample expanded sweep, obtain cymbopogon distans tlc scanning figure, such as Fig. 4 ~ 5.
3.3 methodological studies
3.3.1 linear relationship is investigated：Precision weighs Kaempferol reference substance in right amount, plus every 1ml is made containing 1.058mg in methanol
Solution, be used as reference substance solution A；Precision weighs scopoletin reference substance in right amount, plus every 1ml is made containing the molten of 0.112mg in methanol
Liquid, is used as reference substance solution B；Precision weighs vanillic acid reference substance in right amount, plus solution of every 1ml containing 1.054mg is made in methanol, makees
For reference substance solution C.Draw reference substance A, the μ l of B solution 200,300 μ l respectively with pipette, mix, be used as mixed mark solution.
3.3.1.1 the preparation of scopoletin standard curve：Precision draws the μ l of scopoletin standard liquid 1,2,3,4,5 points respectively
In same GF254On lamellae, using volume ratio as 8:3:0.7 toluene-ethyl acetate-formic acid is solvent, is deployed, and is taken out,
Dry, determine, with point sample volume（μl）For abscissa, peak area（A）For ordinate, standard curve is prepared；Scopoletin standard items
It is in good linear relationship between 0.1126~0.5668 μ g.Regression equation：Y=641.68X+557.86, r=0.9992, knot
Fruit is shown in Table 1.
The scopoletin linear relationship of table 1 is investigated
3.3.1.2 the preparation of Kaempferol standard curve：Precision draws the μ l of Kaempferol standard liquid 0.5,1,1.5,2,2.5
Put respectively in same GF254On lamellae, using volume ratio as 8:3:0.7 toluene-ethyl acetate-formic acid is solvent, exhibition
Open, take out, dry, determine, with point sample volume（μl）For abscissa, peak area（A）For ordinate, standard curve is prepared；Kaempferia galamga
Phenol standard items are in good linear relationship between 0.5304~2.6801 μ g.Regression equation：Y=5755.5X-754.01, r=
0.9990, it the results are shown in Table 2.
The Kaempferol linear relationship of table 2 is investigated
3.3.1.3 the preparation of vanillic acid standard curve：Precision draws the μ l of vanillic acid standard liquid 1,2,3,4,5 points respectively
In same GF254On lamellae, using volume ratio as 7:3:1:0.6 toluene-ethyl acetate-methyl alcohol-formic acid is solvent, exhibition
Open, take out, dry, determine, with point sample volume（μl）For abscissa, peak area（A）For ordinate, standard curve is prepared；Vanilla
Sour standard items are in good linear relationship between 1.0835~5.3819 μ g.Regression equation：Y=1718.1X+4936.2, r
=0.9993, it the results are shown in Table 3.
The vanillic acid linear relationship of table 3 is investigated
3.3.2 stability test：By the μ l of test sample (130916-1-113) 4 of same batch spot interval it is different when
Between, scanned by condition determination, determine each point in different time absorption peak deviations, the results are shown in Table 4.
The stability test result of table 4（n=8）
3.3.3 precision test
By each reference substance solution by certain volume point in GF254On lamellae, by thin layer condition point sample, deploy, take out, dry in the air
Dry, colour developing.By condition of scanning sweep measuring, 5 are the results are shown in Table.
The precision of table 5 investigates result（n=7）
3.3.3 recovery of standard addition is tested：Precision weighs 3 parts of cymbopogon distans powder sample (130916-1), the 1st part
0.5001g, the 2nd part of 0.5011g, the 3rd part of 0.5008g.Addition scopoletin standard items are respectively 0.30mg according to 2.1.1 test samples
Solution manufacturing method is operated, and is determined；Precision weighs cymbopogon distans powder sample（130816-2）3 parts, the 1st part of 0.5023g, the 2nd part
It is respectively that 1.50mg is grasped according to 2.1.1 need testing solutions preparation method that 0.5018g, the 3rd part of 0.5039g, which add Kaempferol standard items,
Make, determine；Precision weighs cymbopogon distans powder（Hetian City the People's Hospital）3 parts, the 1st part of 0.5016g, the 2nd part of 0.5042g, the 3rd part
0.5037g, it is respectively that 3.30mg is operated according to 2.1.1 need testing solutions preparation method to add vanillic acid standard items, is determined.As a result
It is shown in Table 6.
The scopoletin of table 6, Kaempferol, the recovery test result of vanillic acid
3.3.4 the measure of sample size：It is accurate respectively to draw ten batches of need testing solutions, by thin layer condition point sample, expansion,
Take out, dry, develop the color.By condition of scanning sweep measuring, in order to avoid its contingency, the every batch of parallel determination 3 times.Calculate its average value
It the results are shown in Table 7.
The assay result of scopoletin, Kaempferol, vanillic acid in the sample of table 7
4 conclusions
Present embodiment completes discriminating and the assay of cymbopogon distans medicinal material, and its Materia Medica Identification is to use kaempferia galamga
Phenol, scopoletin GF254With plate thin-layer developing, vanillic acid individually uses GF254Lamellae plate deploys, in 25 ± 1 DEG C of room temperature, humidity 41
Reappearance is good under the conditions of ± 1%RH.Assay use tlc-scanning determination cymbopogon distans in Kaempferol, scopoletin and
The content of vanillic acid, by methodological study, stability, precision, recovery of standard addition is preferable.Show this method room temperature 25 ±
1 DEG C, the technical requirements of assay are met under the conditions of 41 ± 1%RH of humidity.Be generally applicable to the medicinal material it is qualitative and quantitative will
Ask.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, although with reference to foregoing reality
Apply example the present invention is described in detail, for a person skilled in the art, it still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent to which part technical characteristic.All essences in the present invention
God is with principle, and any modifications, equivalent substitutions and improvements made etc. should be included within the scope of the present invention.
Claims (5)
1. a kind of quality determining method of cymbopogon distans medicinal material, it is characterised in that:Qualitatively detected including cymbopogon distans medicinal material, determine it
The true and false：
With Kaempferol, scopoletin, vanillic acid composition in ethyl acetate and 25% hydrochloric acid extraction cymbopogon distans medicinal material, after extract solution filtering,
It is evaporated, methanol dissolving is used as need testing solution；
The medicinal material sample spot of Kaempferol, scopoletin mark product and extraction is on same silica GF254 lamellae, and solvent deploys,
Inspected under 365nm uviol lamps, untested medicinal material shows same color with standard items in same position, when Kaempferol, scopoletin expansion, institute
The solvent stated is toluene-ethyl acetate-formic acid, and its volume ratio is 8:3:0.7；
Vanilla acidity scale product and the medicinal material sample spot of extraction are on same silica GF254 lamellae, and solvent expansion, 254nm is ultraviolet
Inspected under lamp, untested medicinal material shows same color with standard items in same position, when vanillic acid deploys, with toluene-ethyl acetate-first
Alcohol-formic acid solvent expansion, its volume ratio is 7:3:1:0.6.
2. the quality determining method of cymbopogon distans medicinal material according to claim 1, it is characterised in that:Also include specific quantitative
Detection method, step is as follows：
1）It is prepared by need testing solution
Cymbopogon distans medicinal powder 5.001g is taken, ethyl acetate 50ml is added, after 25% hydrochloric acid 2.5ml is fully mixed, ultrasonic 60min,
Filtering, is transferred on evaporating dish placement water-bath and is evaporated, add 5ml methanol and be completely dissolved, be used as need testing solution；
2）It is prepared by reference substance solution
Precision weighs Kaempferol standard items 10.58mg, scopoletin standard items 1.12mg and vanillic acid standard items 10.54mg, respectively
Put with methanol constant volume in 10ml volumetric flasks, dissolve, Kaempferol reference substance 1.058mg/ml, scopoletin reference substance 0.112mg/ml,
Vanillic acid reference substance 1.054mg/ml, is used as reference substance solution；
3）Cymbopogon distans thin layer scanning is quantified
The μ l of scopoletin reference substance 2, Kaempferol reference substance 1 μ l are drawn, 10 crowdes of μ l of medicinal material test sample 4 are put in same silica gel respectively
On GF254 lamellaes, solvent expansion is taken out, dried, alchlor-ethanol solution using 1% is uniformly sprayed on as developer
On the lamellae dried, dry, be scanned according to chromatography again, wavelength is 330nm, measure test sample trap integrated value
With reference substance trap integrated value, calculate, produce the content of Kaempferol, scopoletin；Draw the μ l of vanillic acid reference substance 4,10 batches of medicines
The μ l of material test sample 4 are put on same silica GF254 lamellae respectively, are deployed, and are taken out, are dried, molten with 1% alchlor-ethanol
Liquid is uniformly sprayed on the lamellae dried as developer, is dried again, is scanned according to chromatography, and wavelength is 300nm,
Test sample trap integrated value and reference substance trap are measured, calculates, produces the content of vanillic acid.
3. the quality determining method of cymbopogon distans medicinal material according to claim 2, it is characterised in that:Described step 2）In,
During dissolving, solution is placed in ultrasonic wave and handles 60min.
4. the quality determining method of cymbopogon distans medicinal material according to claim 2, it is characterised in that:Described step 3）In,
When Kaempferol, scopoletin expansion, described solvent is toluene-ethyl acetate-formic acid, and its volume ratio is 8:3:0.7；Vanillic acid
During expansion, deployed with toluene-ethyl acetate-methyl alcohol-formic acid solvent, its volume ratio is 7:3:1:0.6.
5. the quality determining method of the cymbopogon distans medicinal material according to claim any one of 1-4, it is characterised in that:Thin layer exhibition
It is opened under the conditions of 21~25 DEG C of room temperature, 40~43RH of air humidity.
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Address after: 830000 the Xinjiang Uygur Autonomous Region Urumqi hi tech Industrial Development Zone (new urban area) 1375 Patentee after: New Qikang Pharmaceutical Co.,Ltd. Address before: 830000 Yingbin Road, Urumqi, the Xinjiang Uygur Autonomous Region 625 Patentee before: XINJIANG QIKANG HABO UIGHUR MEDICINE CO.,LTD. |
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Denomination of invention: A Quality Testing Method for Ru Xiang Cao Medicinal Material Effective date of registration: 20230324 Granted publication date: 20170711 Pledgee: China Construction Bank Corporation Urumqi Youhao North Road Branch Pledgor: New Qikang Pharmaceutical Co.,Ltd. Registration number: Y2023980036259 |