CN105628853A - Quality detection method for medicinal herb herba cymbopogonis - Google Patents
Quality detection method for medicinal herb herba cymbopogonis Download PDFInfo
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- CN105628853A CN105628853A CN201510972088.8A CN201510972088A CN105628853A CN 105628853 A CN105628853 A CN 105628853A CN 201510972088 A CN201510972088 A CN 201510972088A CN 105628853 A CN105628853 A CN 105628853A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The invention discloses a quality detection method for a medicinal herb herba cymbopogonis. The quality detection method is used for true and false identification or quality of the herb by taking content of three components including kaempferol, scopoletin and vanillic acid as the comparison standard. The method, disclosed by the invention, is simple and convenient to operate, clear in chromatographic spots and better in separation effect; through methodological investigation, the method is good in accuracy and repeatability; the established high-efficiency thin-layered chromatography based on three main effective components of herba cymbopogonis can provide certain reference information for quality evaluation of a Uighur medicine Hanchuan Zupa particles, and further provides reference for deeply researching pharmacodynamic material basis and quality control of the Hanchuan Zupa particles.
Description
Technical field
The present invention relates to the qualitative and quantitative discrimination method of medical material, particularly relate to the quality evaluating method of Herba Cymbopogonis medical material.
Background technology
In dimension medicine dyspnea due to cold ancestral's handkerchief granule, Herba Cymbopogonis is as wherein mainly comprising medical material simply, pharmacological action aspect have relieving asthma, antitussive, the effect such as antibacterial. 3, Herba Cymbopogonis has homonym phenomenon, and comparatively obscure always, crude drug " Herba Cymbopogonis " in the dyspnea due to cold ancestral's handkerchief granule obtained in pharmaceutical factory is carried out raw medicinal herbs discriminating, being differentiated by Plant Taxonomy, the Herba Cymbopogonis used in dyspnea due to cold ancestral's handkerchief granule is a kind of quality evaluation to the leaf Radix Euphorbiae Pekinensis (EuphorbiasororiaA) of the Euphorbiaceae Euphorbia annual herb plant.
Herba Cymbopogonis, with herb and fruit medicine, is distributed mainly on the Central Asia and the area such as Xinjiang of China and field, is Uygur's medical science medicinal herbs most in use, and Uygur is called " Su Zhafu ", occupies critical positions in Uygur's medical science, study of pharmacy. This medical material has important medical value, as a kind of conventional medicament with long medication history, function cure mainly for inducing diuresis to remove edema, blood pressure lowering cephalocathartic, relieving asthma, antitussive, antibacterial, rush down under assorted worm. Swell and ache for constipation, frequent micturition, hypertension complicated headache, liver cirrhosis, edema and scabies. Now " to the leaf Radix Euphorbiae Pekinensis " is had only 1997 " Chinese Plants will " 44(3 of certain record) roll up 113 pages, simply describe its plant shape and feature, be not discussed in detail, so research has more basis and Clinical significance of MG.
Summary of the invention
Present invention aims to the deficiencies in the prior art, it is provided that the quality determining method of a kind of easy and simple to handle, stability is strong, precision is high Herba Cymbopogonis medical material.
The purpose of the present invention implements by the following technical programs:
The quality determining method of a kind of Herba Cymbopogonis medical material, in Herba Cymbopogonis medical material, the content of three kinds of compositions of kaempferol, scopoletin, vanillic acid distinguishes the true and false of medical material or quality as comparison standard.
Herba Cymbopogonis medical material detects qualitatively, it is determined that it is true and false:
With kaempferol, scopoletin, vanillic acid composition in ethyl acetate and 25% hydrochloric acid extraction Herba Cymbopogonis medical material, extracting solution is evaporated after filtering, and methanol dissolves, as need testing solution;
The medical material sample spot of kaempferol, scopoletin mark product and extraction is in same silica gel G F254On lamellae, developing solvent launches, and inspects under 365nm uviol lamp, and medical material to be measured and standard substance are at the aobvious same color of same position;
The medical material sample spot of Rhizoma et radix valerianae acidity scale product and extraction is in same silica gel G F254On lamellae, developing solvent launches, and inspects under 254nm uviol lamp, and medical material to be measured and standard substance are at the aobvious same color of same position.
Through preferred developing solvent, the developing solvent described in kaempferol, scopoletin is toluene-ethyl acetate-formic acid, and its volume ratio is 8:3:0.7; When vanillic acid launches, launching with toluene-ethyl acetate-methyl alcohol-formic acid developing solvent, its volume ratio is 7:3:1:0.6
The detection method step of specifically quantitative is as follows:
1) prepared by need testing solution
Take Herba Cymbopogonis medicinal powder 5.001g, add ethyl acetate 50ml, after 25% hydrochloric acid 2.5ml fully mixes, ultrasonic 60min, filters, is transferred on evaporating dish placement water-bath and is evaporated, add 5ml methanol and be completely dissolved, as need testing solution 5ml;
2) prepared by reference substance solution
Precision weighs kaempferol standard substance 10.58mg, scopoletin standard substance 1.12mg and vanillic acid standard substance 10.54mg, put in 10ml volumetric flask by methanol constant volume respectively, dissolve, kaempferol reference substance 1.058mg/ml, scopoletin reference substance 0.112mg/ml, vanillic acid reference substance 1.054mg/ml, as reference substance solution;
3) Herba Cymbopogonis thin-layer qualitative differentiates
Drawing scopoletin reference substance 2 �� l, kaempferol reference substance 1 �� l, test sample 4 �� l puts respectively in same silica gel G F254On lamellae, developing solvent launches, and takes out, dries, inspect under the rearmounted 365nm uviol lamp that develops the color, in test sample chromatograph, on position corresponding with reference substance chromatograph, and the speckle of aobvious same color;
Drawing vanillic acid reference substance 4 �� l, test sample 4 �� l point is in same silica gel G F254On lamellae, developing solvent launches, and takes out, dries, inspect under the rearmounted 254nm uviol lamp that develops the color, on position corresponding with reference substance chromatograph, and the speckle of aobvious same color;
4) Herba Cymbopogonis thin slice scan is quantitative
Drawing scopoletin reference substance 2 �� l, kaempferol reference substance 1 �� l, 10 crowdes of medical material test sample 4 �� l put respectively in same silica gel G F254On lamellae, developing solvent launches, and takes out, dries, aluminum chloride-alcoholic solution using 1% is as developer, uniformly it is sprayed on the lamellae dried, again dries, be scanned according to chromatography, wavelength is 330nm, measure test sample trap integrated value and reference substance trap, calculate, obtain the content of kaempferol, scopoletin;
Draw vanillic acid reference substance 4 �� l, 10 crowdes of medical material test sample 4 �� l to put respectively in same silica gel G F254On lamellae, launch, take out, dry, aluminum chloride-alcoholic solution using 1% is as developer, uniformly it is sprayed on the lamellae dried, again dries, be scanned according to chromatography, wavelength is 300nm, measure test sample trap integrated value and reference substance trap, calculate, obtain the content of vanillic acid.
Preferably, described step 2) in, during dissolving, solution is placed in ultrasound wave and processes 60min.
Preferably, described step 3), 4) in, when kaempferol, scopoletin launch, described developing solvent is toluene-ethyl acetate-formic acid, and its volume ratio is 8:3:0.7; When vanillic acid is opened, launching with toluene-ethyl acetate-methyl alcohol-formic acid developing solvent, its volume ratio is 7:3:1:0.6.
Preferably, thin-layer developing under 21 ~ 25 DEG C of conditions of room temperature, air humidity 40 ~ 43RH.
The present invention adopts hydrochloric ethyl acetate to be extracted into point-score, and extractability is strong.
The method have the benefit that
The present invention provides a kind of Uygur medicinal herbs most in use--the quality evaluating method of Herba Cymbopogonis medical material, it completes thin layer chromatography qualitative identification and the assay of Herba Cymbopogonis, and wherein assay is the content using tlc-scanning determination kaempferol, scopoletin and vanillic acid. Bright through methodology proof list, the method precision, repeatability, stability and average recovery are all good, it was shown that the method meets the technology requirement of assay.
The inventive method is easy and simple to handle, chromatograph clear spot, separating effect are better, methodological study can show that its precision and repeatability are good, set up with the high performance thin layer chromatography of in Herba Cymbopogonis 3 kinds of principle active component, the quality evaluation for tieing up medicine dyspnea due to cold ancestral's handkerchief granule can provide certain reference information. Also the new drug development for going deep into dyspnea due to cold ancestral's handkerchief particle medicinal effective substance basic research and its kind provides reference value.
Accompanying drawing explanation
Fig. 1 is kaempferol (A), the mixed mark of scopoletin (B) and 10 batch samples thin-layer developing figure when ultraviolet 365nm;
Fig. 2 is scopoletin mark product (B), kaempferol mark product (A) and 10 batch samples thin-layer developing figure when ultraviolet 365nm;
Fig. 3 is Rhizoma et radix valerianae acidity scale product (C) and 10 batch samples thin-layer developing figure, C when ultraviolet 254nm are Rhizoma et radix valerianae acidity scale product;
Fig. 4 is kaempferol (A), the mixed mark of scopoletin (B) and 10 batch samples thin layer quantitative scanning figure when ultraviolet 330nm;
Fig. 5 is vanillic acid (C) and 10 batch samples thin layer quantitative scanning figure when ultraviolet 300nm.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is merely to illustrate and explains the present invention, is not intended to limit the present invention.
1. instrument and reagent
1.1 instruments
CAMAGTLCSCANNER3 type thin-layer chromatogram scanner (Switzerland); The semi-automatic point sample instrument of CAMAGLINOMAT5 (Switzerland); CAMAGREPROSTAR3 (Switzerland); Silica gel G F254High Performance Thin plate (Anhui Liangchen Silicon Material Co., Ltd.); XS-105 type electronic balance (Switzerland Mei Le-Tuo benefit company); XS105 type 100,000/electronic balance (Switzerland prunus mume (sieb.) sieb.et zucc. Teller-Tuo Li company); KQ-200KDE type ultrasonic cleaner (Kunshan Ultrasonic Instruments Co., Ltd.); HH-S4Digital display thermostat water bath (Medical Instruments factory of Jintan City)
1.2 reagents
Herba Cymbopogonis medical material: (Xinjiang Qi Kanghabowei medicine limited company, lot number: 090202-1,120916-3,130816-2,130916-1,130916-1-113,140305-2,140227-1), Uygur medicine hospital of crow city, Hetian City the People's Hospital and field individuality Wei Yao pharmacy.
All the other reagent are analytical pure.
2. method
2.1 Herba Cymbopogonis thin layer chromatography qualitative identification
2.1.1 the preparation of need testing solution: precision weighs above each batch of medical material 5.001g, it is respectively placed in 100ml and jumps a queue in conical flask, add ethyl acetate 50ml, 25% hydrochloric acid 2.5ml, mixing supersound process 60min, filters, and filtrate is evaporated, residue adds 5ml methanol makes dissolving, obtains need testing solution (1,2,3,4,5,6,7,8,9,10).
2.1.2 the preparation of reference substance solution: it is appropriate that precision weighs kaempferol reference substance, adds methanol and makes every 1ml solution containing 1.058mg, as reference substance solution A; It is appropriate that precision weighs scopoletin reference substance, adds methanol and makes every 1ml solution containing 0.112mg, as reference substance solution B; It is appropriate that precision weighs vanillic acid reference substance, adds methanol and makes every 1ml solution containing 1.054mg, as reference substance solution C.
2.1.3 thin layer chromatography condition: draw scopoletin reference substance 2 �� l, kaempferol reference substance 1 �� l, test sample 4 �� l puts respectively on same silica GF254 lamellae, with volume ratio be 8:3:0.7 toluene-ethyl acetate-formic acid for developing solvent, launch, take out, dry, inspect under the rearmounted 365nm uviol lamp that develops the color; In test sample chromatograph, on position corresponding with reference substance chromatograph, the speckle of aobvious same color. Drawing vanillic acid reference substance 4 �� l, test sample 4 �� l point is in same silica gel G F254On lamellae, with volume ratio be 7:3:1:0.6 toluene-ethyl acetate-methyl alcohol-formic acid for developing solvent, launch, take out, dry, inspect under the rearmounted 254nm uviol lamp that develops the color; On position corresponding with reference substance chromatograph, the speckle of aobvious same color.
2.2 Herba Cymbopogonis TLC scanning method are quantitative
Test according to thin layer chromatography, pipette samples solution 4 �� l, reference substance solution A 1 �� l, reference substance B solution 2 �� l, put in same silica gel G F254On lamellae, with volume ratio be 8:3:0.7 toluene-ethyl acetate-formic acid for developing solvent, launch, take out, dry, spray with 1% aluminum chloride-alcoholic solution colour developing, dry, be scanned according to chromatography, wavelength is 330nm, measure test sample trap integrated value and reference substance trap, calculate, obtain the content of kaempferol in Herba Cymbopogonis, scopoletin. Vanillic acid assay: pipette samples 4 �� l solution, reference substance C solution 4 �� l, puts in same silica gel G F254On lamellae, with volume ratio be 7:3:1:0.6 toluene-ethyl acetate-methyl alcohol-formic acid for developing solvent, consistent with above operational approach, scanning wavelength is 300nm, measure test sample trap integrated value and reference substance trap, calculate, obtain the content of vanillic acid in Herba Cymbopogonis.
3. result
3.1 Herba Cymbopogonis thin layer chromatography qualitative identification
The standard solution prepared and 10 batches of need testing solutions are launched and inspection knowledge under uviol lamp (254nm) by above-mentioned thin layer chromatography condition point sample, it is thus achieved that standard solution and Herba Cymbopogonis extract thin-layer chromatogram, such as Fig. 1.
3.2 Herba Cymbopogonis tlc scanning figure
The mixed mark solution prepared and 10 batches of need testing solutions are launched and inspection knowledge under uviol lamp (365nm) and (254nm) by above-mentioned thin layer chromatography condition point sample, it is thus achieved that Herba Cymbopogonis thin-layer chromatogram, such as Fig. 1 ~ 3. By the mixed mark solution prepared and 10 batches of need testing solutions by above-mentioned thin layer chromatography condition point sample expanded sweep, it is thus achieved that Herba Cymbopogonis tlc scanning figure, such as Fig. 4 ~ 5.
3.3 methodological studies
3.3.1 linear relationship is investigated: it is appropriate that precision weighs kaempferol reference substance, adds methanol and makes every 1ml solution containing 1.058mg, as reference substance solution A; It is appropriate that precision weighs scopoletin reference substance, adds methanol and makes every 1ml solution containing 0.112mg, as reference substance solution B; It is appropriate that precision weighs vanillic acid reference substance, adds methanol and makes every 1ml solution containing 1.054mg, as reference substance solution C. Draw reference substance A, B solution 200 �� l, 300 �� l respectively with pipet, mixing, as mixed mark solution.
3.3.1.1 the preparation of scopoletin standard curve: accurate scopoletin standard solution 1,2,3,4, the 5 �� l that draws puts respectively in same GF254On lamellae, with volume ratio be 8:3:0.7 toluene-ethyl acetate-formic acid for developing solvent, launch, take out, dry, measure, with point sample volume (�� l) for abscissa, peak area (A) is vertical coordinate, prepares standard curve; Scopoletin standard substance are good linear relationship between 0.1126��0.5668 �� g. Regression equation: Y=641.68X+557.86, r=0.9992, result is in Table 1.
Table 1 scopoletin linear relationship is investigated
3.3.1.2 the preparation of kaempferol standard curve: accurate kaempferol standard solution 0.5,1,1.5,2, the 2.5 �� l that draws puts respectively in same GF254On lamellae, with volume ratio be 8:3:0.7 toluene-ethyl acetate-formic acid for developing solvent, launch, take out, dry, measure, with point sample volume (�� l) for abscissa, peak area (A) is vertical coordinate, prepares standard curve; Kaempferol standard substance are good linear relationship between 0.5304��2.6801 �� g. Regression equation: Y=5755.5X-754.01, r=0.9990, result is in Table 2.
Table 2 kaempferol linear relationship is investigated
3.3.1.3 the preparation of vanillic acid standard curve: accurate vanillic acid standard solution 1,2,3,4, the 5 �� l that draws puts respectively in same GF254On lamellae, with volume ratio be 7:3:1:0.6 toluene-ethyl acetate-methyl alcohol-formic acid for developing solvent, launch, take out, dry, measure, with point sample volume (�� l) for abscissa, peak area (A) is vertical coordinate, prepares standard curve; Vanillic acid standard substance are good linear relationship between 1.0835��5.3819 �� g. Regression equation: Y=1718.1X+4936.2, r=0.9993, result is in Table 3.
Table 3 vanillic acid linear relationship is investigated
3.3.2 stability test: by the time different for the speckle interval of test sample (130916-1-113) the 4 �� l of same batch, scan by condition determination, measures each point in different time absorption peak deviations, and result is in Table 4.
Table 4 stability test result (n=8)
3.3.3 precision test
Each reference substance solution is pressed certain volume point in GF254On lamellae, by thin layer condition point sample, expansion, take out, dry, develop the color. By condition of scanning sweep measuring, result is in Table 5.
Table 5 precision investigates result (n=7)
3.3.3 recovery of standard addition test: precision weighs Herba Cymbopogonis powder sample (130916-1) 3 parts, the 1st part of 0.5001g, the 2nd part of 0.5011g, the 3rd part of 0.5008g. Add scopoletin standard substance respectively 0.30mg to operate according to 2.1.1 need testing solution preparation method, measure; Precision weighs Herba Cymbopogonis powder sample (130816-2) 3 parts, and the 1st part of 0.5023g, the 2nd part of 0.5018g, the 3rd part of 0.5039g add kaempferol standard substance respectively 1.50mg and operate according to 2.1.1 need testing solution preparation method, measure; Precision weighs 3 parts of Herba Cymbopogonis powder (Hetian City the People's Hospital), the 1st part of 0.5016g, the 2nd part of 0.5042g, the 3rd part of 0.5037g, adds vanillic acid standard substance respectively 3.30mg and operates according to 2.1.1 need testing solution preparation method, measures. Result is in Table 6.
Table 6 scopoletin, kaempferol, vanillic acid recovery test result
3.3.4 the mensuration of sample size: respectively precision draws ten batches of need testing solutions, by thin layer condition point sample, expansion, takes out, dries, develops the color. By condition of scanning sweep measuring, in order to avoid its occasionality, the every batch of parallel assay 3 times. Calculate its average results in Table 7.
The assay result of scopoletin, kaempferol, vanillic acid in table 7 sample
4 conclusions
This detailed description of the invention completes discriminating and the assay of Herba Cymbopogonis medical material, and its Materia Medica Identification is to adopt kaempferol, scopoletin GF254With plate thin-layer developing, vanillic acid individually adopts GF254Lamellae plate launches, and room temperature 25 �� 1 DEG C, when humidity 41 �� 1%RH, repeatability is good. Assay adopts the content of kaempferol, scopoletin and vanillic acid in tlc-scanning determination Herba Cymbopogonis, and by methodological study, stability, precision, recovery of standard addition is better. Show that the method is room temperature 25 �� 1 DEG C, meets the technology requirement of assay when humidity 41 �� 1%RH. It is generally applicable to the requirement that this medical material is qualitative and quantitative.
The foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art, technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature carries out equivalent replacement. All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (7)
1. the quality determining method of a Herba Cymbopogonis medical material, it is characterised in that: in Herba Cymbopogonis medical material, the content of three kinds of compositions of kaempferol, scopoletin, vanillic acid distinguishes the true and false of medical material or quality as comparison standard.
2. the quality determining method of Herba Cymbopogonis medical material according to claim 1, it is characterised in that: Herba Cymbopogonis medical material detects qualitatively, it is determined that its true and false:
With kaempferol, scopoletin, vanillic acid composition in ethyl acetate and 25% hydrochloric acid extraction Herba Cymbopogonis medical material, extracting solution is evaporated after filtering, and methanol dissolves, as need testing solution;
The medical material sample spot of kaempferol, scopoletin mark product and extraction is in same silica gel G F254On lamellae, developing solvent launches, and inspects under 365nm uviol lamp, and medical material to be measured and standard substance are at the aobvious same color of same position;
The medical material sample spot of Rhizoma et radix valerianae acidity scale product and extraction is in same silica gel G F254On lamellae, developing solvent launches, and inspects under 254nm uviol lamp, and medical material to be measured and standard substance are at the aobvious same color of same position.
3. the quality determining method of Herba Cymbopogonis medical material according to claim 2, it is characterised in that: when kaempferol, scopoletin launch, described developing solvent is toluene-ethyl acetate-formic acid, and its volume ratio is 8:3:0.7; When vanillic acid launches, launching with toluene-ethyl acetate-methyl alcohol-formic acid developing solvent, its volume ratio is 7:3:1:0.6.
4. the quality determining method of Herba Cymbopogonis medical material according to claim 2, it is characterised in that: concrete quantitative detection method step is as follows:
1) prepared by need testing solution
Take Herba Cymbopogonis medicinal powder 5.001g, add ethyl acetate 50ml, after 25% hydrochloric acid 2.5ml fully mixes, ultrasonic 60min, filters, is transferred on evaporating dish placement water-bath and is evaporated, add 5ml methanol and be completely dissolved, as need testing solution 5ml;
2) prepared by reference substance solution
Precision weighs kaempferol standard substance 10.58mg, scopoletin standard substance 1.12mg and vanillic acid standard substance 10.54mg, put in 10ml volumetric flask by methanol constant volume respectively, dissolve, kaempferol reference substance 1.058mg/ml, scopoletin reference substance 0.112mg/ml, vanillic acid reference substance 1.054mg/ml, as reference substance solution;
3) Herba Cymbopogonis thin-layer qualitative differentiates
Drawing scopoletin reference substance 2 �� l, kaempferol reference substance 1 �� l, test sample 4 �� l puts respectively in same silica gel G F254On lamellae, developing solvent launches, and takes out, dries, inspect under the rearmounted 365nm uviol lamp that develops the color, in test sample chromatograph, on position corresponding with reference substance chromatograph, and the speckle of aobvious same color;
Drawing vanillic acid reference substance 4 �� l, test sample 4 �� l point is in same silica gel G F254On lamellae, developing solvent launches, and takes out, dries, inspect under the rearmounted 254nm uviol lamp that develops the color, on position corresponding with reference substance chromatograph, and the speckle of aobvious same color;
4) Herba Cymbopogonis thin slice scan is quantitative
Drawing scopoletin reference substance 2 �� l, kaempferol reference substance 1 �� l, 10 crowdes of medical material test sample 4 �� l put respectively in same silica gel G F254On lamellae, developing solvent launches, and takes out, dries, aluminum chloride-alcoholic solution using 1% is as developer, uniformly it is sprayed on the lamellae dried, again dries, be scanned according to chromatography, wavelength is 330nm, measure test sample trap integrated value and reference substance trap integrated value, calculate, obtain the content of kaempferol, scopoletin;
Draw vanillic acid reference substance 4 �� l, 10 crowdes of medical material test sample 4 �� l to put respectively in same silica gel G F254On lamellae, launch, take out, dry, aluminum chloride-alcoholic solution using 1% is as developer, uniformly it is sprayed on the lamellae dried, again dries, be scanned according to chromatography, wavelength is 300nm, measure test sample trap integrated value and reference substance trap, calculate, obtain the content of vanillic acid.
5. the quality determining method of Herba Cymbopogonis medical material according to claim 4, it is characterised in that: described step 2) in, during dissolving, solution is placed in ultrasound wave and processes 60min.
6. the quality determining method of Herba Cymbopogonis medical material according to claim 4, it is characterised in that: described step 3), 4) in, when kaempferol, scopoletin launch, described developing solvent is toluene-ethyl acetate-formic acid, and its volume ratio is 8:3:0.7; When vanillic acid launches, launching with toluene-ethyl acetate-methyl alcohol-formic acid developing solvent, its volume ratio is 7:3:1:0.6.
7. the quality determining method of the Herba Cymbopogonis medical material according to any one of claim 2-6, it is characterised in that: thin-layer developing under 21 ~ 25 DEG C of conditions of room temperature, air humidity 40 ~ 43RH.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106153781A (en) * | 2016-08-29 | 2016-11-23 | 新疆奇康哈博维药股份有限公司 | The extracting method of active polyphenol in Herba Cymbopogonis |
| CN106353421A (en) * | 2016-08-29 | 2017-01-25 | 新疆奇康哈博维药股份有限公司 | Method of measuring contents of three polyphenol ingredients in Cymbopogon distans |
| CN110850048A (en) * | 2019-10-15 | 2020-02-28 | 贵州医科大学 | Quality control method of euphorbia helioscopia |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101618119A (en) * | 2008-07-04 | 2010-01-06 | 天津中新药业集团股份有限公司第六中药厂 | Quality standard of Sinopanax formosanus pills |
| CN101700267A (en) * | 2009-11-27 | 2010-05-05 | 广州白云山和记黄埔中药有限公司 | Detection method of persimmon leaf quality |
| EP2808017A1 (en) * | 2013-05-27 | 2014-12-03 | King Saud University | Extract and isolated compounds from Convolvulus austroaegyptiacus for use as anti-ulcer agent and/or anti-Heliobacter pylori agent |
| CN105136966A (en) * | 2015-06-29 | 2015-12-09 | 成都中医药大学 | Quality detection method for Liangfu pill-like preparations |
-
2015
- 2015-12-22 CN CN201510972088.8A patent/CN105628853B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101618119A (en) * | 2008-07-04 | 2010-01-06 | 天津中新药业集团股份有限公司第六中药厂 | Quality standard of Sinopanax formosanus pills |
| CN101700267A (en) * | 2009-11-27 | 2010-05-05 | 广州白云山和记黄埔中药有限公司 | Detection method of persimmon leaf quality |
| EP2808017A1 (en) * | 2013-05-27 | 2014-12-03 | King Saud University | Extract and isolated compounds from Convolvulus austroaegyptiacus for use as anti-ulcer agent and/or anti-Heliobacter pylori agent |
| CN105136966A (en) * | 2015-06-29 | 2015-12-09 | 成都中医药大学 | Quality detection method for Liangfu pill-like preparations |
Non-Patent Citations (7)
| Title |
|---|
| E. SAWICKI 等: "TLC-fluorimetric analysis for atmospheric scopoletin", 《MICROCHEMICAL JOURNAL》 * |
| GODDEN B 等: "Towards elucidation of the lignin degradation pathway in actinomycetes", 《MICROBIOLOGY》 * |
| 刘文炜 等: "荨麻属植物大蝎子草化学成分及质量标准研究", 《中国药学杂志》 * |
| 刘霞 等: "两种狗脊属药材的鉴别研究", 《中华中医药杂志》 * |
| 地力努尔•吐尔逊江 等: "对叶大戟草的生药鉴别", 《中药材》 * |
| 张维库 等: "对叶大戟化学成分的研究", 《中国中药杂志》 * |
| 杜怡昊 等: "川楝子中香草酸含量测定研究", 《天然产物研究与开发》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106153781A (en) * | 2016-08-29 | 2016-11-23 | 新疆奇康哈博维药股份有限公司 | The extracting method of active polyphenol in Herba Cymbopogonis |
| CN106353421A (en) * | 2016-08-29 | 2017-01-25 | 新疆奇康哈博维药股份有限公司 | Method of measuring contents of three polyphenol ingredients in Cymbopogon distans |
| CN110850048A (en) * | 2019-10-15 | 2020-02-28 | 贵州医科大学 | Quality control method of euphorbia helioscopia |
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Address after: 830000 the Xinjiang Uygur Autonomous Region Urumqi hi tech Industrial Development Zone (new urban area) 1375 Patentee after: New Qikang Pharmaceutical Co.,Ltd. Address before: 830000 Yingbin Road, Urumqi, the Xinjiang Uygur Autonomous Region 625 Patentee before: XINJIANG QIKANG HABO UIGHUR MEDICINE CO.,LTD. |
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Denomination of invention: A Quality Testing Method for Ru Xiang Cao Medicinal Material Effective date of registration: 20230324 Granted publication date: 20170711 Pledgee: China Construction Bank Corporation Urumqi Youhao North Road Branch Pledgor: New Qikang Pharmaceutical Co.,Ltd. Registration number: Y2023980036259 |