CN100464188C - Quality control method of medicinal preparation for treating lypemania - Google Patents
Quality control method of medicinal preparation for treating lypemania Download PDFInfo
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- CN100464188C CN100464188C CNB2005102008668A CN200510200866A CN100464188C CN 100464188 C CN100464188 C CN 100464188C CN B2005102008668 A CNB2005102008668 A CN B2005102008668A CN 200510200866 A CN200510200866 A CN 200510200866A CN 100464188 C CN100464188 C CN 100464188C
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Abstract
A quality control method for the Chinese medicine used to treat depression includes such steps as checking its characteristics, discrimination to cablin potchouli herb, atractylodes rhizome, katsumadai seed and tangerine peel, and measuring the content of hesperidin.
Description
Technical field: the present invention relates to a kind of method of quality control for the treatment of medicine (Yu Shuda) preparation of depression, belong to the technical field of medicine being carried out quality control.
Background technology: depression is a kind of common phrenoblabia, is one of principal disease of human high incidence.External investigation finds that depressed point prevalence is 4.3%, and lifetime prevalence is 8%～20%.According to scholarly forecast, the incidence of disease of China's depression also will be improved largely than the eighties.At present antidepressants mostly are Western medicine greatly on the foreign market, but the part patient is not good to its reaction, and multiple spinoff and contraindication are arranged, and patient can not be tolerated and be difficult to reach satisfactory effect, and recurrence rate is also high, and various antidepressants all might bring out dry mad.Depressive disorder is a kind of affective disorders, and suppressing with depressed, retardation of thinking and psychomotor activity clinically is feature, belongs to the category of the traditional Chinese medical science " strongly fragrant disease ".The applicant developed the YUSHUDA ZHIJI prescription finished by Pogostemon cablin, dried orange peel, cardamom, in one's early teens, rhizoma atractylodis (bran stir-fry) form, prescription is with light clear fragrance, the dry product of bitter warm nature are main the composition, all medicine compatibilities are gone into middle Jiao, the dampness elimination of being amusing, Xie Yu regulates the flow of vital energy.Studies show that the antidepressant effect of YUSHUDA ZHIJI is remarkable, spinoff is little; Quality controllable, stable performance is a kind of preparation of very promising treatment by Chinese herbs depressive disorder; The applicant has submitted patented claim to State Patent Office, application number: 200510200474.1.The applicant finds in the development process of whole medicine: if this medicine does not have the strict quality standard, the product quality reliability that obtains is just not high, and result of treatment can be not obvious; Think and reduce drug side-effect by the clinical efficacy that improves this medicine, must formulate a reliable quality standard.
Summary of the invention:
The objective of the invention is to: a kind of method of quality control for the treatment of the YUSHUDA ZHIJI of depression is provided, and said preparation comprises all possible compound preparation of capsule, tablet, granule.The present invention is according to the contained chemical constitution of prescription taste of traditional Chinese medicine, preparation technology and formulation, quality control projects such as content assaying method, discrimination method, proterties, inspection have been studied and defined, controlling the quality of YUSHUDA ZHIJI effectively, thereby guarantee the clinical efficacy of this preparation.
The YUSHUDA ZHIJI of treatment depression of the present invention is by Pogostemon cablin 1000g, dried orange peel 334g, cardamom 500g, 500g and rhizoma atractylodis (bran stir-fry) 666g make in one's early teens, its preparation method is: five tastes medicinal material boiling four times, each 2 hours, amount of water was 14 times of medicinal material amount; Collecting decoction, filter, being concentrated into relative density is the clear cream of 1.04-1.05 (50 ℃), adds ethanol and makes and contain the alcohol amount and reach 70%, refrigeration (5～10 ℃) is spent the night, filter, filtrate recycling ethanol, and be concentrated into the clear cream that relative density is 1.32～1.35 (50 ℃), drying under reduced pressure (70 ℃), dried cream powder is broken into fine powder, adds the appropriate amount of auxiliary materials mixing again, makes corresponding preparation according to conventional method.
Method of quality control of the present invention mainly comprises proterties, discriminating, inspection and assay project, and wherein the proterties item should meet the relevant regulations under each preparation item; Differentiate be to Pogostemon cablin, rhizoma atractylodis, in one's early teens, the discriminating of dried orange peel; Check that item should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000; The assay item is for measuring Determination of Hesperidin Content in the preparation.
Wherein, the discrimination method of Pogostemon cablin is to be contrast with the Pogostemon cablin control medicinal material, and with toluene: ethyl acetate: normal hexane: formic acid=2: 4: 8: 0.1 is the thin layer discrimination method of developping agent.
The discrimination method of rhizoma atractylodis is to be contrast with the rhizoma atractylodis control medicinal material, and with toluene: ethyl acetate: normal hexane=1.5: 0.3: 7 is the thin layer discrimination method of developping agent.
Discrimination method in one's early teens is to be contrast with control medicinal material in one's early teens, the plain reference substance of Alpinia japonica, and with methenyl choloride: methyl alcohol=9: 0.5 is the thin layer discrimination method of developping agent.
The discrimination method of dried orange peel is to be contrast with dried orange peel control medicinal material, aurantiamarin reference substance, and with ethyl acetate: methyl alcohol: the upper solution of water=6: 1.5: 3 is the thin layer discrimination method of developping agent.
Concrete discrimination method comprises the part or all of of following project:
(1) get YUSHUDA ZHIJI, porphyrize claims 3.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 3.0g of Pogostemon cablin, make the Pogostemon cablin blank solution with method; Other gets Pogostemon cablin control medicinal material 3.0g, shines medicinal material solution in pairs with legal system; According to the test of an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 4 μ l of blank solution, control medicinal material solution 4 μ l put in same silica G F
254On the thin layer plate, with toluene: ethyl acetate: normal hexane: formic acid=2: 4: 8: 0.1 is developping agent, launches, and takes out, and dries, and puts under the 365nm ultraviolet lamp and inspects; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; Negative sample does not then show;
(2) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 4.0g of rhizoma atractylodis, make the rhizoma atractylodis blank solution with method; Other gets rhizoma atractylodis control medicinal material 0.5g, shines medicinal material solution in pairs with legal system; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 10 μ l of blank solution, control medicinal material solution 6 μ l, point is on same silica gel g thin-layer plate, with toluene: ethyl acetate: normal hexane=1.5: 0.3: 7 be a developping agent, expansion, take out, dry, spray is with 5% phosphomolybdic acid-ethanolic solution, and 105 ℃ to be heated to spot colour developing clear; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color; Negative sample does not then show;
(3) get YUSHUDA ZHIJI, porphyrize claims 2.0g, adds methyl alcohol 15ml, puts and heats jolting 5 minutes in the water-bath, filter, filtrate is put in the separating funnel, adds the ethyl acetate jolting and extracts 2 times, each 10ml, merge extract, evaporate to dryness, residue add ethyl acetate 1ml makes dissolving, as need testing solution; Get blank in one's early teens preparation 2.0g, make blank solution in one's early teens with method; Get control medicinal material 2.0g in one's early teens, shine medicinal material solution in pairs with legal system; Other gets the plain reference substance of Alpinia japonica, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 10 μ l of control medicinal material solution, reference substance solution 2 μ l, point is on same silica gel g thin-layer plate, with methenyl choloride: methyl alcohol=9: 0.5 is developping agent, launches, and takes out, dry, put under the 365nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color; Negative sample does not then show;
(4) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methyl alcohol 25ml, and sonicated is 30 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and filtrate is as need testing solution; Get the blank preparation 4.0g of dried orange peel, make the dried orange peel blank solution with method; Get dried orange peel control medicinal material 2.0g, shine medicinal material solution in pairs with legal system; Other gets the aurantiamarin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 6 μ l of control medicinal material solution, reference substance solution 8 μ l, point is on same silica gel g thin-layer plate, with ethyl acetate: methyl alcohol: the upper solution of water=6: 1.5: 3 is a developping agent, launches, take out, dry, spray is put under the 365nm ultraviolet lamp and is inspected with the aluminium choride test solution; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show an identical yellow-green fluorescence spot; Negative sample does not then show.
The Determination of Hesperidin Content assay method is to be contrast with the aurantiamarin reference substance, and with acetonitrile: 1% acetic acid aqueous solution=20: 80 is the high performance liquid chromatography of moving phase.
Concrete content assaying method is:
According to 2000 editions one appendix VI D of Chinese Pharmacopoeia high effective liquid chromatography for measuring:
Chromatographic condition and system suitability test are filling agent with octadecylsilane chemically bonded silica; Acetonitrile: 1% acetic acid aqueous solution=20: 80 is a moving phase; The detection wavelength is 283nm; 30 ℃ of column temperatures; Number of theoretical plate calculates by the aurantiamarin peak should be not less than 2000;
It is an amount of that the preparation precision of reference substance solution takes by weighing the aurantiamarin reference substance, adds methyl alcohol and make the solution that every 1ml contains 0.4mg, promptly;
The about 0.2g of YUSHUDA ZHIJI is got in the preparation of need testing solution, porphyrize, and accurate the title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, and sonicated is 1 hour under power 150W, frequency 20kHz condition, put coldly, claim again to decide weight, supply the weight that subtracts mistake, shake up with methyl alcohol, filter, precision is measured subsequent filtrate 30ml, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with 0.45 μ m miillpore filter;
Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing of determination method inject liquid chromatograph, measure, and the external standard two-point method calculates, promptly;
The every least unit of YUSHUDA ZHIJI contains dried orange peel with aurantiamarin C
28H
34O
15Meter must not be less than 6.70mg.
Described method of quality control comprises:
Proterties: medicine or its content show light brown, gas perfume (or spice), mildly bitter flavor;
Differentiate: (1) gets YUSHUDA ZHIJI, and porphyrize claims 3.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 3.0g of Pogostemon cablin, make the Pogostemon cablin blank solution with method; Other gets Pogostemon cablin control medicinal material 3.0g, shines medicinal material solution in pairs with legal system; According to the test of an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 4 μ l of blank solution, control medicinal material solution 4 μ l put in same silica G F
254On the thin layer plate, with toluene: ethyl acetate: normal hexane: formic acid=2: 4: 8: 0.1 is developping agent, launches, and takes out, and dries, and puts under the 365nm ultraviolet lamp and inspects; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; Negative sample does not then show;
(2) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 4.0g of rhizoma atractylodis, make the rhizoma atractylodis blank solution with method; Other gets rhizoma atractylodis control medicinal material 0.5g, shines medicinal material solution in pairs with legal system; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 10 μ l of blank solution, control medicinal material solution 6 μ l, point is on same silica gel g thin-layer plate, with toluene: ethyl acetate: normal hexane=1.5: 0.3: 7 be a developping agent, expansion, take out, dry, spray is with 5% phosphomolybdic acid-ethanolic solution, and 105 ℃ to be heated to spot colour developing clear; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color; Negative sample does not then show;
(3) get YUSHUDA ZHIJI, porphyrize claims 2.0g, adds methyl alcohol 15ml, puts and heats jolting 5 minutes in the water-bath, filter, filtrate is put in the separating funnel, adds the ethyl acetate jolting and extracts 2 times, each 10ml, merge extract, evaporate to dryness, residue add ethyl acetate 1ml makes dissolving, as need testing solution; Get blank in one's early teens preparation 2.0g, make blank solution in one's early teens with method; Get control medicinal material 2.0g in one's early teens, shine medicinal material solution in pairs with legal system; Other gets the plain reference substance of Alpinia japonica, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 10 μ l of control medicinal material solution, reference substance solution 2 μ l, point is on same silica gel g thin-layer plate, with methenyl choloride: methyl alcohol=9: 0.5 is developping agent, launches, and takes out, dry, put under the 365nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color; Negative sample does not then show;
(4) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methyl alcohol 25ml, and sonicated is 30 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and filtrate is as need testing solution; Get the blank preparation 4.0g of dried orange peel, make the dried orange peel blank solution with method; Get dried orange peel control medicinal material 2.0g, shine medicinal material solution in pairs with legal system; Other gets the aurantiamarin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 6 μ l of control medicinal material solution, reference substance solution 8 μ l, point is on same silica gel g thin-layer plate, with ethyl acetate: methyl alcohol: the upper solution of water=6: 1.5: 3 is a developping agent, launches, take out, dry, spray is put under the 365nm ultraviolet lamp and is inspected with the aluminium choride test solution; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show an identical yellow-green fluorescence spot; Negative sample does not then show;
Check: should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000;
Assay: shine 2000 editions one appendix VI D of Chinese Pharmacopoeia high effective liquid chromatography for measuring:
Chromatographic condition and system suitability test are filling agent with octadecylsilane chemically bonded silica; Acetonitrile: 1% acetic acid aqueous solution=20: 80 is a moving phase; The detection wavelength is 283nm; 30 ℃ of column temperatures; Number of theoretical plate calculates by the aurantiamarin peak should be not less than 2000;
It is an amount of that the preparation precision of reference substance solution takes by weighing the aurantiamarin reference substance, adds methyl alcohol and make the solution that every 1ml contains 0.4mg, promptly;
The about 0.2g of YUSHUDA ZHIJI is got in the preparation of need testing solution, porphyrize, and accurate the title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, and sonicated is 1 hour under power 150W, frequency 20kHz condition, put coldly, claim again to decide weight, supply the weight that subtracts mistake, shake up with methyl alcohol, filter, precision is measured subsequent filtrate 30ml, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with 0.45 μ m miillpore filter;
Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing of determination method inject liquid chromatograph, measure, and the external standard two-point method calculates, promptly;
The every least unit of YUSHUDA ZHIJI contains dried orange peel with aurantiamarin C
28H
34O
15Meter must not be less than 6.70mg.
Compared with prior art, method of quality control specificity of the present invention is strong, favorable reproducibility, and the precision height, good stability has guaranteed the clinical efficacy of this preparation effectively, has reached the goal of the invention of effective control drug quality.
For science and the rationality of verifying method of quality control of the present invention, carried out following experimental study.
One, differentiates
1, the feature of contained Pogostemon cablin is differentiated in the side of being: the discrimination method under the pharmacopeia Pogostemon cablin item is the discriminating of volatile oil, this preparation is an aqueous extraction-alcohol precipitation technology, therefore the described discrimination method of pharmacopeia is inapplicable, the list of references report, by test, obtained medicinal material and test sample and had corresponding feature spot, negative sample is noiseless, so adopt thin layer of the present invention to differentiate.
2, the feature of contained rhizoma atractylodis is differentiated in the side of being: the discrimination method under the pharmacopeia rhizoma atractylodis item is the discriminating of volatile oil,
This preparation is an aqueous extraction-alcohol precipitation technology, so the described discrimination method of pharmacopeia is inapplicable, by test, has obtained medicinal material and test sample and has had corresponding feature spot, and negative sample is noiseless, so adopt thin layer of the present invention to differentiate.
3, contained feature is in one's early teens differentiated in the side of being: according to the discrimination method of pharmacopeia under in one's early teens, feminine gender has interference, the list of references report, select different developping agents for use, by test, obtain medicinal material and test sample and had corresponding feature spot, be equipped with corresponding spot with the plain reference substance corresponding positions of Alpinia japonica, negative sample is noiseless, so adopt thin layer of the present invention to differentiate.
4, the feature of contained dried orange peel is differentiated in the side of being: with reference to the discrimination method under the pharmacopeia dried orange peel item, negative sample is noiseless, so adopt thin layer of the present invention to differentiate.
Two, check
By " regulation under each preparation item of appendix of Chinese pharmacopoeia version in 2000 is carried out following inspection to three batch samples:
Weight differential is checked: according to weight differential under an appendix ID of Pharmacopoeia of People's Republic of China version in 2000 the tablet item three batch samples are checked, be the results are shown in Table 1.
Check disintegration time limited: according to inspection technique disintegration time limited (an appendix XII of Pharmacopoeia of People's Republic of China version in 2000 A), three batch samples are checked, be the results are shown in Table 1.
Table 1 weight differential, disintegration time limited check result
| Sample number | 040811 | 040812 | 040813 |
| Weight differential | Up to specification | Up to specification | Up to specification |
| Disintegration time limited (branch) | 17 | 17 | 17 |
Heavy metal is checked: according to heavy metal inspection technique (an appendix IX of Pharmacopoeia of People's Republic of China version in 2000 E), three batch samples 040811,040812,040813 are checked, do not detected, so exclude content of the present invention.
Arsenic salt is checked: according to arsenic salt inspection technique (an appendix IX of Pharmacopoeia of People's Republic of China version in 2000 F), three batch samples 040811,040812,040813 are checked, do not detected, so exclude content of the present invention.
The organic chlorine agriculture chemicals residual quantity is checked: according to organic chlorine agriculture chemicals determination of residual amount method (an appendix IX of Pharmacopoeia of People's Republic of China version in 2000 Q) three batch samples 040811,040812,040813 are checked, the result shows, three batch sample benzene hexachloride (BHC), D.D.T. (dichloro-diphenyl-trichloroethane) (DDT) content are all up to specification, so exclude content of the present invention.
Three, assay
According to high-efficient liquid phase technique (an appendix VI of Pharmacopoeia of People's Republic of China version in 2000 D).
Instrument and reagent:
Instrument: Waters Delta 600 pumps; Waters 2478 Dual λ Absobance Detector UV-detector;
Waters Millennium32 workstation; Ultrasonic oscillator (power 150W, frequency 20kHz).
Reagent: acetonitrile is a chromatographically pure, and methyl alcohol is that top grade is pure, and it is pure that all the other reagent are analysis, and water is the deionization redistilled water; 0.45 μ m filtering membrane (Millipore), aurantiamarin reference substance (available from the 0721-200010 of Nat'l Pharmaceutical ﹠ Biological Products Control Institute).
1, the preparation of test sample: see this instructions text content assaying method.
2, the preparation of reference substance solution: see this instructions text content assaying method.
3, chromatographic condition
Chromatographic column: 150 * 4.6mm 5 μ Inertsil ODS-3, moving phase: acetonitrile-1% acetic acid aqueous solution (20: 80), detect wavelength: 283nm, column temperature: 30 ℃, flow velocity: 1ml/min.
Once selected the described mobile phase methanol-acetic acid of pharmacopeia-water (35: 4: 61) for use, the aurantiamarin separating effect is not good in the preparation, so transposing moving phase, when selecting acetonitrile-1% acetic acid aqueous solution (20: 80) for use for moving phase, the aurantiamarin peak shape is better, does not have other composition and disturbs, so list content of the present invention in.
4, linear relationship is investigated
Aurantiamarin reference substance 1,2,3,4,5,6,7,8 Ma that accurate absorption concentration is 0.4042mg/ml inject liquid chromatograph, measure.The result shows that aurantiamarin is good linear relationship between 0.4042～3.2336 Therewith, and γ=0.9997 sees Table 2.
Table 2 aurantiamarin linear relationship is investigated data
| Sample size | 1μl | 2μl | 3μl | 4μl | 5μl | 6μl | 7μl | 8μl |
| (μg) | 0.4042 | 0.8084 | 1.2126 | 1.6168 | 2.0210 | 2.4252 | 2.8294 | 3.2336 |
| Peak area | 604157 | 1101802 | 1794577 | 2416015 | 3009257 | 3649007 | 4296760 | 4916229 |
Y＝1540839.8X-79158.0 γ＝0.9997
5, blank test
Get the blank preparation of dried orange peel (going the dried orange peel preparation), accurately claim decide 1.0g, make the dried orange peel blank solution with the preparation method of text need testing solution by full prescription, sample introduction under above-mentioned HPLC chromatographic condition, the result shows that feminine gender is noiseless.
6, precision is investigated
Get formulation samples (040511), porphyrize, precision claims to conclude a contract or treaty puts 1.0g in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, sonicated (power 150W, frequency 20kHz) 1 hour, put coldly, claim again to decide weight, supply the weight that subtracts mistake with methyl alcohol, shake up, filter, precision is measured subsequent filtrate 10ml evaporate to dryness, and residue adds methyl alcohol makes dissolving, and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with miillpore filter (0.45 μ m).The accurate respectively need testing solution 4 μ l that draw, sample introduction is measured its peak area under above-mentioned HPLC chromatographic condition, and the external standard two-point method calculates its content.The results are shown in Table 3.
Table 3 precision is investigated
The result shows that the instrument precision relative standard deviation is 1.58%, shows that instrument precision is good.
7, Wen Dingxing investigation
Get formulation samples (040511), porphyrize, precision claims to conclude a contract or treaty puts 1.0g in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, sonicated (power 150W, frequency 20kHz) 1 hour, put coldly, claim again to decide weight, supply the weight that subtracts mistake with methyl alcohol, shake up, filter, precision is measured subsequent filtrate 10ml evaporate to dryness, and residue adds methyl alcohol makes dissolving, and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with miillpore filter (0.45 μ m).The accurate respectively need testing solution 4 μ l that draw, sample introduction is measured its peak area under above-mentioned HPLC chromatographic condition, and the external standard two-point method calculates its content.The results are shown in Table 4.
Table 4 study on the stability
The result shows that its relative standard deviation is 1.58%, shows that sample was stable in 7 hours.
8, repeatability is investigated
Get formulation samples (040511), porphyrize, the accurate title, decided 5 parts, every part of about 0.3g, put in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, sonicated (power 150W, frequency 20kHz) 1 hour is put coldly, claims to decide weight again, supply the weight that subtracts mistake with methyl alcohol, shake up, filter, precision is measured subsequent filtrate 20ml evaporate to dryness, residue adds methyl alcohol makes dissolving, and is transferred in the 5ml measuring bottle, adds methyl alcohol to scale, shakes up, filter, get subsequent filtrate and filter, promptly with miillpore filter (0.45 μ m).The accurate respectively need testing solution 4 μ l that draw, sample introduction is measured its peak area under above-mentioned HPLC chromatographic condition, and the external standard two-point method calculates its content.The results are shown in Table 5.
Table 5 repeatability is investigated
The result shows that its relative standard deviation is that RSD% is 1.50%, shows that the sample reappearance is good.
9, the investigation of extracting method
Different solvents, Different Extraction Method, different extraction time are investigated, the need testing solution that Different Extraction Method makes is measured under above-mentioned high-efficient liquid phase chromatogram condition, the result shows that ultrasonic 1 hour extraction ratio of methyl alcohol is the highest, extracts the most complete.The results are shown in Table 6,7,8,9,10.
9.1 the investigation of different solvents extracting method
Get formulation samples (040511), porphyrize, precision claims to conclude a contract or treaty puts 4.5g in the tool plug conical flask, accurate respectively methyl alcohol, ethyl acetate, each 50ml of absolute ethyl alcohol of adding, close plug claims to decide weight, sonicated (power 150W, frequency 20kHz) 45 minutes is put cold, claim again to decide weight, supply the weight that subtracts mistake, shake up with solvent, filter, get subsequent filtrate and filter, promptly get need testing solution with miillpore filter (0.45 μ m), inject liquid chromatograph, measure, the external standard two-point method calculates its content.The results are shown in Table 6.
The investigation of table 6 different solvents extracting method
| Sample number | (g) weighs | Sample size (μ l) | Peak area | Content of hesperidin (mg/g preparation) |
| Ethyl acetate | 4.6136 | 20 | 42088 | - |
| Methyl alcohol | 5.3022 | 2 | 3612484 | 10.39 |
| Absolute ethyl alcohol | 4.6186 | 20 | 2267405 | 0.80 |
The result shows that the methanol extraction rate is the highest.
9.2 different time methyl alcohol heating and refluxing extraction
Get formulation samples (040511), porphyrize, precision claims to conclude a contract or treaty 4.5g, add methyl alcohol 100ml, reflux was taken out after different time, be cooled to room temperature, shake up, filter, filtrate is transferred in the 100ml measuring bottle, adds methyl alcohol to scale, shakes up, filter, get subsequent filtrate and filter, promptly get need testing solution with miillpore filter (0.45 μ m), inject liquid chromatograph, measure, the external standard two-point method calculates its content.The results are shown in Table 7.
The investigation of table 7 different heating time
| (g) weighs | Heat time heating time | Sample size (μ l) | Peak area | Content of hesperidin (mg/g preparation) |
| 4.6177 | 1 hour | 5 | 2837667 | 7.71 |
| 4.6131 | 3 hours | 5 | 2798305 | 7.62 |
| 4.6168 | 5 hours | 5 | 3333923 | 8.88 |
| 4.0923 | 7 hours | 5 | 2661274 | 8.22 |
The result shows that 5 hours extraction ratios of methyl alcohol heating are the highest, and content of hesperidin is the 8.88mg/g preparation.
9.3 extract after the different time reflux
The about 4.5g of sample thief (040511), porphyrize, the accurate title, decide, precision adds entry 50ml, and reflux was taken out after different time, be cooled to room temperature, shake up, filter, the accurate subsequent filtrate 30ml that draws puts in the separating funnel, add the ethyl acetate jolting and extract 3 times, each 30ml merges extract, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly get need testing solution, inject liquid chromatograph with miillpore filter (0.45 μ m), measure, the external standard two-point method calculates its content.The results are shown in Table 8.
The investigation that extracts after the table 8 different time reflux
| (g) weighs | Heat time heating time | Sample size (μ l) | Peak area | Content of hesperidin (mg/g preparation) |
| 4.6232 | 1 hour | 1 | 2272200 | 2.65 |
| 4.6277 | 3 hours | 1 | 2326503 | 2.70 |
| 4.6240 | 5 hours | 1 | 2546579 | 2.92 |
The result shows, the water reflux extracts extraction ratio after 5 hours the highest, and content of hesperidin is the 2.92mg/g preparation.
9.4 Different Extraction Method
The about 4.5g of sample thief (040511), porphyrize, accurate claim surely, the accurate methyl alcohol 50ml that adds adopts ultrasonic 45 minutes extracting method and the cold soaking method of spending the night to extract respectively, above-mentioned two kinds of extracting method solution are filtered, get subsequent filtrate and filter, promptly get need testing solution, inject liquid chromatograph with miillpore filter (0.45 μ m), measure, the external standard two-point method calculates its content.The results are shown in Table 9.
The investigation of table 9 Different Extraction Method
| (g) weighs | Extracting method | Sample size (μ l) | Peak area | Content of hesperidin (mg/g preparation) |
| 5.3022 | Ultrasonic 45 minutes | 2 | 3612484 | 10.39 |
| 4.6229 | Cold soaking spends the night | 2 | 919832 | 3.95 |
The result shows not, and the ultrasonic method extraction ratio is the highest.Content of hesperidin is the 10.39mg/g preparation.
9.5 the investigation of ultrasonic extraction different extraction times
The about 0.3g of sample thief (040511), porphyrize, the accurate title, decide, put in the tool plug conical flask accurate methyl alcohol 50ml, the close plug of adding, claim to decide weight, sonicated (power 150W, frequency 20kHz) is after different time, put coldly, claim again to decide weight, supply the weight that subtracts mistake with methyl alcohol, shake up, filter, accurate absorption subsequent filtrate is quantitative, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly get need testing solution, inject liquid chromatograph with miillpore filter (0.45 μ m), measure, the external standard two-point method calculates its content.The results are shown in Table 10.
The investigation of table 10 different extraction times
| (g) weighs | The ultrasonic Extraction time | Sample size (μ l) | Get subsequent filtrate amount (ml) | Peak area | Content of hesperidin (mg/g preparation) |
| 0.3889 | 15 minutes | 5 | 30 | 2725911 | 7.36 |
| 0.3476 | 30 minutes | 4 | 30 | 2002486 | 7.93 |
| 0.3460 | 45 minutes | 4 | 20 | 1686080 | 10.38 |
| 0.3472 | 60 minutes | 4 | 30 | 2846451 | 10.70 |
| 0.3458 | 90 minutes | 4 | 20 | 1531749 | 9.62 |
The result shows that 60 minutes extraction ratios of ultrasonic Extraction are the highest.Content of hesperidin is the 10.70mg/g preparation.
The investigation result of comprehensive said extracted method, as can be known, the methyl alcohol ultrasonic Extraction was extracted the most complete in 60 minutes.
10, the recovery is investigated
Adopt the test of application of sample absorption method.(040511) 5 part in the sample that precision takes by weighing known content adds the aurantiamarin reference substance respectively, presses under the text assay item preparation need testing solution and measures, and with the following formula calculate recovery rate, the results are shown in Table 11.
Recovery computing formula: (C-A)/B * 100%=recovery %
A: sample is contained measured
B: add pure product amount
C: measured value
Table 11 recovery is investigated
The determination of recovery rates result: average recovery rate is 100.94%, relative standard deviation RSD%=2.20%.
11, the mensuration of sample
Sample thief 040510,040511,040512, the preparation method makes need testing solution with text need testing solution preparation.Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing inject liquid chromatograph, measure under the chromatographic condition of drafting, and the external standard two-point method calculates Determination of Hesperidin Content in the preparation.The results are shown in Table 12.
The mensuration of table 12 sample
| Sample number | Sample weighting amount (g) | Peak area (1) | Peak area (2) | Average peak area | Content of hesperidin (mg/ sheet) |
| 040510 | 0.1847 | 2949863 | 3051753 | 3000808 | 9.24 |
| 040510 | 0.1806 | 2862567 | 2937082 | 2899824.5 | 9.13 |
| 040511 | 0.1723 | 2547425 | 2566273 | 2556849 | 8.52 |
| 040511 | 0.1566 | 2768504 | 2786177 | 2777340.5 | 8.56 |
| 040512 | 0.1617 | 2578017 | 2586661 | 2582339 | 8.99 |
| 040512 | 0.1745 | 2358026 | 2359397 | 2358711.5 | 9.05 |
Conclusion: according to the surely lower limit of the data of three batch samples, get then its 80%, the every least unit of YUSHUDA ZHIJI contain dried orange peel with aurantiamarin (C
28H
34O
15) meter, must not be less than 6.70mg.
Embodiment:
Embodiments of the invention 1:
Proterties: medicine or its content show light brown, gas perfume (or spice), mildly bitter flavor.
Differentiate: (1) gets YUSHUDA ZHIJI, and porphyrize claims 3.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 3.0g of Pogostemon cablin, make the Pogostemon cablin blank solution with method; Other gets Pogostemon cablin control medicinal material 3.0g, shines medicinal material solution in pairs with legal system; According to the test of an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 4 μ l of blank solution, control medicinal material solution 4 μ l put in same silica G F
254On the thin layer plate, with toluene: ethyl acetate: normal hexane: formic acid=2: 4: 8: 0.1 is developping agent, launches, and takes out, and dries, and puts under the 365nm ultraviolet lamp and inspects; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; Negative sample does not then show.
(2) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 4.0g of rhizoma atractylodis, make the rhizoma atractylodis blank solution with method; Other gets rhizoma atractylodis control medicinal material 0.5g, shines medicinal material solution in pairs with legal system; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 10 μ l of blank solution, control medicinal material solution 6 μ l, point is on same silica gel g thin-layer plate, with toluene: ethyl acetate: normal hexane=1.5: 0.3: 7 be a developping agent, expansion, take out, dry, spray is with 5% phosphomolybdic acid, one ethanolic solution, and 105 ℃ to be heated to spot colour developing clear; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color; Negative sample does not then show.
(3) get YUSHUDA ZHIJI, porphyrize claims 2.0g, adds methyl alcohol 15ml, puts and heats jolting 5 minutes in the water-bath, filter, filtrate is put in the separating funnel, adds the ethyl acetate jolting and extracts 2 times, each 10ml, merge extract, evaporate to dryness, residue add ethyl acetate 1ml makes dissolving, as need testing solution; Get blank in one's early teens preparation 2.0g, make blank solution in one's early teens with method; Get control medicinal material 2.0g in one's early teens, shine medicinal material solution in pairs with legal system; Other gets the plain reference substance of Alpinia japonica, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 10 μ l of control medicinal material solution, reference substance solution 2 μ l, point is on same silica gel g thin-layer plate, with methenyl choloride: methyl alcohol=9: 0.5 is developping agent, launches, and takes out, dry, put under the 365nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color; Negative sample does not then show.
(4) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methyl alcohol 25ml, and sonicated is 30 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and filtrate is as need testing solution; Get the blank preparation 4.0g of dried orange peel, make the dried orange peel blank solution with method; Get dried orange peel control medicinal material 2.0g, shine medicinal material solution in pairs with legal system; Other gets the aurantiamarin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 6 μ l of control medicinal material solution, reference substance solution 8 μ l, point is on same silica gel g thin-layer plate, with ethyl acetate: methyl alcohol: the upper solution of water=6: 1.5: 3 is a developping agent, launches, take out, dry, spray is put under the 365nm ultraviolet lamp and is inspected with the aluminium choride test solution; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show an identical yellow-green fluorescence spot; Negative sample does not then show.
Check: should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000.
Assay: shine 2000 editions one appendix VI D of Chinese Pharmacopoeia high effective liquid chromatography for measuring:
Chromatographic condition and system suitability test are filling agent with octadecylsilane chemically bonded silica; Acetonitrile: 1% acetic acid aqueous solution=20: 80 is a moving phase; The detection wavelength is 283nm; 30 ℃ of column temperatures; Number of theoretical plate calculates by the aurantiamarin peak should be not less than 2000;
It is an amount of that the preparation precision of reference substance solution takes by weighing the aurantiamarin reference substance, adds methyl alcohol and make the solution that every 1ml contains 0.4mg, promptly;
The about 0.2g of YUSHUDA ZHIJI is got in the preparation of need testing solution, porphyrize, and accurate the title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, and sonicated is 1 hour under power 150W, frequency 20kHz condition, put coldly, claim again to decide weight, supply the weight that subtracts mistake, shake up with methyl alcohol, filter, precision is measured subsequent filtrate 30ml, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with 0.45 μ m miillpore filter;
Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing of determination method inject liquid chromatograph, measure, and the external standard two-point method calculates, promptly;
The every least unit of YUSHUDA ZHIJI contains dried orange peel with aurantiamarin C
28H
34O
15Meter must not be less than 6.70mg.
Embodiments of the invention 2:
Proterties: medicine or its content show light brown, gas perfume (or spice), mildly bitter flavor.
Differentiate: get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 4.0g of rhizoma atractylodis, make the rhizoma atractylodis blank solution with method; Other gets rhizoma atractylodis control medicinal material 0.5g, shines medicinal material solution in pairs with legal system; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 10 μ l of blank solution, control medicinal material solution 6 μ l, point is on same silica gel g thin-layer plate, with toluene: ethyl acetate: normal hexane=1.5: 0.3: 7 be a developping agent, expansion, take out, dry, spray is with 5% phosphomolybdic acid-ethanolic solution, and 105 ℃ to be heated to spot colour developing clear; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color; Negative sample does not then show.
Check: should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000.
Assay: shine 2000 editions one appendix VI D of Chinese Pharmacopoeia high effective liquid chromatography for measuring:
Chromatographic condition and system suitability test are filling agent with octadecylsilane chemically bonded silica; Acetonitrile: 1% acetic acid aqueous solution=20: 80 is a moving phase; The detection wavelength is 283nm; 30 ℃ of column temperatures; Number of theoretical plate calculates by the aurantiamarin peak should be not less than 2000;
It is an amount of that the preparation precision of reference substance solution takes by weighing the aurantiamarin reference substance, adds methyl alcohol and make the solution that every 1ml contains 0.4mg, promptly;
The about 0.2g of YUSHUDA ZHIJI is got in the preparation of need testing solution, porphyrize, and accurate the title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, and sonicated is 1 hour under power 150W, frequency 20kHz condition, put coldly, claim again to decide weight, supply the weight that subtracts mistake, shake up with methyl alcohol, filter, precision is measured subsequent filtrate 30ml, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with 0.45 μ m miillpore filter;
Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing of determination method inject liquid chromatograph, measure, and the external standard two-point method calculates, promptly;
The every least unit of YUSHUDA ZHIJI contains dried orange peel with aurantiamarin C
28H
34O
15Meter must not be less than 6.70mg.
Embodiments of the invention 3:
Proterties: medicine or its content show light brown, gas perfume (or spice), mildly bitter flavor;
Differentiate: get YUSHUDA ZHIJI, porphyrize claims 2.0g, adds methyl alcohol 15ml, puts and heats jolting 5 minutes in the water-bath, filter, filtrate is put in the separating funnel, adds the ethyl acetate jolting and extracts 2 times, each 10ml, merge extract, evaporate to dryness, residue add ethyl acetate 1ml makes dissolving, as need testing solution; Get blank in one's early teens preparation 2.0g, make blank solution in one's early teens with method; Get control medicinal material 2.0g in one's early teens, shine medicinal material solution in pairs with legal system; Other gets the plain reference substance of Alpinia japonica, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 10 μ l of control medicinal material solution, reference substance solution 2 μ l, point is on same silica gel g thin-layer plate, with methenyl choloride: methyl alcohol=9: 0.5 is developping agent, launches, and takes out, dry, put under the 365nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color; Negative sample does not then show.
Check: should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000.
Assay: shine 2000 editions one appendix VID high effective liquid chromatography for measuring of Chinese Pharmacopoeia:
Chromatographic condition and system suitability test are filling agent with octadecylsilane chemically bonded silica; Acetonitrile: 1% acetic acid aqueous solution=20: 80 is a moving phase; The detection wavelength is 283nm; 30 ℃ of column temperatures; Number of theoretical plate calculates by the aurantiamarin peak should be not less than 2000;
It is an amount of that the preparation precision of reference substance solution takes by weighing the aurantiamarin reference substance, adds methyl alcohol and make the solution that every 1ml contains 0.4mg, promptly;
The about 0.2g of YUSHUDA ZHIJI is got in the preparation of need testing solution, porphyrize, and accurate the title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, and sonicated is 1 hour under power 150W, frequency 20kHz condition, put coldly, claim again to decide weight, supply the weight that subtracts mistake, shake up with methyl alcohol, filter, precision is measured subsequent filtrate 30ml, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with 0.45 μ m miillpore filter;
Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing of determination method inject liquid chromatograph, measure, and the external standard two-point method calculates, promptly;
The every least unit of YUSHUDA ZHIJI contains dried orange peel with aurantiamarin C
28H
34O
15Meter must not be less than 6.70mg.
Embodiments of the invention 4:
Proterties: medicine or its content show light brown, gas perfume (or spice), mildly bitter flavor.
Differentiate: (1) gets YUSHUDA ZHIJI, and porphyrize claims 3.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 3.0g of Pogostemon cablin, make the Pogostemon cablin blank solution with method; Other gets Pogostemon cablin control medicinal material 3.0g, shines medicinal material solution in pairs with legal system; According to the test of an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 4 μ l of blank solution, control medicinal material solution 4 μ l put in same silica G F
254On the thin layer plate, with toluene: ethyl acetate: normal hexane: formic acid=2: 4: 8: 0.1 is developping agent, launches, and takes out, and dries, and puts under the 365nm ultraviolet lamp and inspects; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; Negative sample does not then show.
(2) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methyl alcohol 25ml, and sonicated is 30 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and filtrate is as need testing solution; Get the blank preparation 4.0g of dried orange peel, make the dried orange peel blank solution with method; Get dried orange peel control medicinal material 2.0g, shine medicinal material solution in pairs with legal system; Other gets the aurantiamarin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 6 μ l of control medicinal material solution, reference substance solution 8 μ l, point is on same silica gel g thin-layer plate, with ethyl acetate: methyl alcohol: the upper solution of water=6: 1.5: 3 is a developping agent, launches, take out, dry, spray is put under the 365nm ultraviolet lamp and is inspected with the aluminium choride test solution; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show an identical yellow-green fluorescence spot; Negative sample does not then show.
Check: should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000.
Embodiments of the invention 5:
Proterties: medicine or its content show light brown, gas perfume (or spice), mildly bitter flavor.
Check: should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000.
Assay: shine 2000 editions one appendix VI D of Chinese Pharmacopoeia high effective liquid chromatography for measuring:
Chromatographic condition and system suitability test are filling agent with octadecylsilane chemically bonded silica; Acetonitrile: 1% acetic acid aqueous solution=20: 80 is a moving phase; The detection wavelength is 283nm; 30 ℃ of column temperatures; Number of theoretical plate calculates by the aurantiamarin peak should be not less than 2000;
It is an amount of that the preparation precision of reference substance solution takes by weighing the aurantiamarin reference substance, adds methyl alcohol and make the solution that every 1ml contains 0.4mg, promptly;
The about 0.2g of YUSHUDA ZHIJI is got in the preparation of need testing solution, porphyrize, and accurate the title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, and sonicated is 1 hour under power 150W, frequency 20kHz condition, put coldly, claim again to decide weight, supply the weight that subtracts mistake, shake up with methyl alcohol, filter, precision is measured subsequent filtrate 30ml, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with 0.45 μ m miillpore filter;
Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing of determination method inject liquid chromatograph, measure, and the external standard two-point method calculates, promptly;
The every least unit of YUSHUDA ZHIJI contains dried orange peel with aurantiamarin C
28H
34O
15Meter must not be less than 6.70mg.
Claims (4)
1.[claim 1] a kind of method of quality control for the treatment of the YUSHUDA ZHIJI of depression, described preparation is by Pogostemon cablin 1000g, dried orange peel 334g, cardamom 500g, 500g and bran are fried all compound preparations that comprise capsule, tablet, granule that rhizoma atractylodis 666g makes in one's early teens, it is characterized in that: described method of quality control comprises proterties, discriminating, inspection and assay project, and wherein the proterties item should meet the relevant regulations under each preparation item; Differentiate be to Pogostemon cablin, rhizoma atractylodis, in one's early teens, the discriminating of dried orange peel, the discriminating of Pogostemon cablin is to be contrast with the Pogostemon cablin control medicinal material, with toluene: ethyl acetate: normal hexane: formic acid=2: 4: 8: 0.1 is the thin-layer chromatography discrimination method of developping agent; The discriminating of rhizoma atractylodis is to be contrast with the rhizoma atractylodis control medicinal material, and with toluene: ethyl acetate: normal hexane=1.5: 0.3: 7 is the thin layer discrimination method of developping agent; Discriminating in one's early teens is to be contrast with control medicinal material in one's early teens, the plain reference substance of Alpinia japonica, and with methenyl choloride: methyl alcohol=9: 0.5 is the thin layer discrimination method of developping agent; The discriminating of dried orange peel is to be contrast with dried orange peel control medicinal material, aurantiamarin reference substance, and with ethyl acetate: methyl alcohol: the upper solution of water=6: 1.5: 3 is the thin layer discrimination method of developping agent; Check that item should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000; The assay item is for measuring Determination of Hesperidin Content in the preparation, and it is to be contrast with the aurantiamarin reference substance that Determination of Hesperidin Content is measured, and with acetonitrile: 1% acetic acid aqueous solution=20: 80 is the high performance liquid chromatography of moving phase.
2.[claim 2] according to the method for quality control of the YUSHUDA ZHIJI of the described treatment depression of claim 1, it is characterized in that: concrete discrimination method is:
(1) get YUSHUDA ZHIJI, porphyrize claims 3.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 3.0g of Pogostemon cablin, make the Pogostemon cablin blank solution with method; Other gets Pogostemon cablin control medicinal material 3.0g, shines medicinal material solution in pairs with legal system; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 4 μ l of blank solution, control medicinal material solution 4 μ l, point is on same silica GF254 thin layer plate, with toluene: ethyl acetate: normal hexane: formic acid=2: 4: 8: 0.1 is developping agent, launches, and takes out, dry, put under the 365nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; Negative sample does not then show;
(2) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 4.0g of rhizoma atractylodis, make the rhizoma atractylodis blank solution with method; Other gets rhizoma atractylodis control medicinal material 0.5g, shines medicinal material solution in pairs with legal system; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 10 μ l of blank solution, control medicinal material solution 6 μ l, point is on same silica gel g thin-layer plate, with toluene: ethyl acetate: normal hexane=1.5: 0.3: 7 be a developping agent, expansion, take out, dry, spray is with 5% phosphomolybdic acid-ethanolic solution, and 105 ℃ to be heated to spot colour developing clear; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color; Negative sample does not then show;
(3) get YUSHUDA ZHIJI, porphyrize claims 2.0g, adds methyl alcohol 15ml, puts and heats jolting 5 minutes in the water-bath, filter, filtrate is put in the separating funnel, adds the ethyl acetate jolting and extracts 2 times, each 10ml, merge extract, evaporate to dryness, residue add ethyl acetate 1ml makes dissolving, as need testing solution; Get blank in one's early teens preparation 2.0g, make blank solution in one's early teens with method; Get control medicinal material 2.0g in one's early teens, shine medicinal material solution in pairs with legal system; Other gets the plain reference substance of Alpinia japonica, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 10 μ l of control medicinal material solution, reference substance solution 2 μ l, point is on same silica gel g thin-layer plate, with methenyl choloride: methyl alcohol=9: 0.5 is developping agent, launches, and takes out, dry, put under the 365nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color; Negative sample does not then show;
(4) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methyl alcohol 25ml, and sonicated is 30 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and filtrate is as need testing solution; Get the blank preparation 4.0g of dried orange peel, make the dried orange peel blank solution with method; Get dried orange peel control medicinal material 2.0g, shine medicinal material solution in pairs with legal system; Other gets the aurantiamarin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 6 μ l of control medicinal material solution, reference substance solution 8 μ l, point is on same silica gel g thin-layer plate, with ethyl acetate: methyl alcohol: the upper solution of water=6: 1.5: 3 is a developping agent, launches, take out, dry, spray is put under the 365nm ultraviolet lamp and is inspected with the aluminium choride test solution; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show an identical yellow-green fluorescence spot; Negative sample does not then show.
3.[claim 3] according to the method for quality control of the YUSHUDA ZHIJI of the described treatment depression of claim 1, it is characterized in that: concrete content assaying method is:
According to 2000 editions one appendix VI D of Chinese Pharmacopoeia high effective liquid chromatography for measuring:
Chromatographic condition and system suitability test are filling agent with octadecylsilane chemically bonded silica; Acetonitrile: 1% acetic acid aqueous solution=20: 80 is a moving phase; The detection wavelength is 283nm; 30 ℃ of column temperatures; Number of theoretical plate calculates by the aurantiamarin peak should be not less than 2000;
It is an amount of that the preparation precision of reference substance solution takes by weighing the aurantiamarin reference substance, and add methyl alcohol and make the solution that every 1ml contains 0.4mg,
Promptly;
The about 0.2g of YUSHUDA ZHIJI is got in the preparation of need testing solution, porphyrize, and accurate the title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, and sonicated is 1 hour under power 150W, frequency 20kHz condition, put coldly, claim again to decide weight, supply the weight that subtracts mistake, shake up with methyl alcohol, filter, precision is measured subsequent filtrate 30ml, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with 0.45 μ m miillpore filter;
Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing of determination method inject liquid chromatograph, measure, and the external standard two-point method calculates, promptly;
The every least unit of YUSHUDA ZHIJI contains dried orange peel with aurantiamarin C
28H
34O
15Meter must not be less than 6.70mg.
4.[claim 4] according to the method for quality control of the YUSHUDA ZHIJI of claim 1,2 or 3 described treatment depression, it is characterized in that: described method of quality control is:
Proterties: medicine or its content show light brown, gas perfume (or spice), mildly bitter flavor;
Differentiate: (1) gets YUSHUDA ZHIJI, and porphyrize claims 3.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 3.0g of Pogostemon cablin, make the Pogostemon cablin blank solution with method; Other gets Pogostemon cablin control medicinal material 3.0g, shines medicinal material solution in pairs with legal system; According to the test of an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 4 μ l of blank solution, control medicinal material solution 4 μ l put in same silica G F
254On the thin layer plate, with toluene: ethyl acetate: normal hexane: formic acid=2: 4: 8: 0.1 is developping agent, launches, and takes out, and dries, and puts under the 365nm ultraviolet lamp and inspects; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; Negative sample does not then show;
(2) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methenyl choloride 25ml, and sonicated is 10 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and the filtrate evaporate to dryness adds the 1ml ethyl acetate and makes dissolving, as need testing solution; Get the blank preparation 4.0g of rhizoma atractylodis, make the rhizoma atractylodis blank solution with method; Other gets rhizoma atractylodis control medicinal material 0.5g, shines medicinal material solution in pairs with legal system; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, each 10 μ l of blank solution, control medicinal material solution 6 μ l, point is on same silica gel g thin-layer plate, with toluene: ethyl acetate: normal hexane=1.5: 0.3: 7 be a developping agent, expansion, take out, dry, spray is with 5% phosphomolybdic acid-ethanolic solution, and 105 ℃ to be heated to spot colour developing clear; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color; Negative sample does not then show;
(3) get YUSHUDA ZHIJI, porphyrize claims 2.0g, adds methyl alcohol 15ml, puts and heats jolting 5 minutes in the water-bath, filter, filtrate is put in the separating funnel, adds the ethyl acetate jolting and extracts 2 times, each 10ml, merge extract, evaporate to dryness, residue add ethyl acetate 1ml makes dissolving, as need testing solution; Get blank in one's early teens preparation 2.0g, make blank solution in one's early teens with method; Get control medicinal material 2.0g in one's early teens, shine medicinal material solution in pairs with legal system; Other gets the plain reference substance of Alpinia japonica, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 10 μ l of control medicinal material solution, reference substance solution 2 μ l, point is on same silica gel g thin-layer plate, with methenyl choloride: methyl alcohol=9: 0.5 is developping agent, launches, and takes out, dry, put under the 365nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color; Negative sample does not then show;
(4) get YUSHUDA ZHIJI, porphyrize claims 4.0g, adds methyl alcohol 25ml, and sonicated is 30 minutes under power 150W, frequency 20kHz condition, is cooled to room temperature, filters, and filtrate is as need testing solution; Get the blank preparation 4.0g of dried orange peel, make the dried orange peel blank solution with method; Get dried orange peel control medicinal material 2.0g, shine medicinal material solution in pairs with legal system; Other gets the aurantiamarin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; Test according to an appendix VIB of Pharmacopoeia of People's Republic of China version in 2000 thin-layered chromatography, draw need testing solution, blank solution, each 6 μ l of control medicinal material solution, reference substance solution 8 μ l, point is on same silica gel g thin-layer plate, with ethyl acetate: methyl alcohol: the upper solution of water=6: 1.5: 3 is a developping agent, launches, take out, dry, spray is put under the 365nm ultraviolet lamp and is inspected with the aluminium choride test solution; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the fluorescence spot of same color; With the corresponding position of reference substance chromatogram on, show an identical yellow-green fluorescence spot; Negative sample does not then show;
Check: should meet the relevant regulations under each preparation item of appendix of Chinese Pharmacopoeia version in 2000;
Assay: shine 2000 editions one appendix VI D of Chinese Pharmacopoeia high effective liquid chromatography for measuring:
Chromatographic condition and system suitability test are filling agent with octadecylsilane chemically bonded silica; Acetonitrile: 1% acetic acid aqueous solution=20: 80 is a moving phase; The detection wavelength is 283nm; 30 ℃ of column temperatures; Number of theoretical plate calculates by the aurantiamarin peak should be not less than 2000;
It is an amount of that the preparation precision of reference substance solution takes by weighing the aurantiamarin reference substance, adds methyl alcohol and make the solution that every 1ml contains 0.4mg, promptly;
The about 0.2g of YUSHUDA ZHIJI is got in the preparation of need testing solution, porphyrize, and accurate the title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 50ml that adds, close plug claims to decide weight, and sonicated is 1 hour under power 150W, frequency 20kHz condition, put coldly, claim again to decide weight, supply the weight that subtracts mistake, shake up with methyl alcohol, filter, precision is measured subsequent filtrate 30ml, evaporate to dryness, with dissolve with methanol and be transferred in the 5ml measuring bottle, add methyl alcohol to scale, shake up, filter, get subsequent filtrate and filter, promptly with 0.45 μ m miillpore filter;
Accurate respectively reference substance solution 3 μ l, 6 μ l and the need testing solution 8 μ l of drawing of determination method inject liquid chromatograph, measure, and the external standard two-point method calculates, promptly;
The every least unit of YUSHUDA ZHIJI contains dried orange peel with aurantiamarin C
28H
34O
15Meter must not be less than 6.70mg.
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| CN101596296B (en) * | 2008-06-02 | 2011-08-17 | 北京亚东生物制药有限公司 | Method for controlling quality of infant antidiarrheal granules |
| CN103142630A (en) * | 2013-03-21 | 2013-06-12 | 中国医学科学院药用植物研究所 | Application of hesperidin or derivatives thereof as antidepressant drug |
| CN104547873A (en) * | 2015-02-02 | 2015-04-29 | 刘平 | Method for preparing traditional Chinese medicine oral instant film for treating depression |
| CN110376326B (en) * | 2019-07-25 | 2021-05-28 | 河北中医学院 | Multi-region thin layer identification and distinguishing method for pericarpium citri reticulatae and immature bitter orange medicinal materials |
| CN111487361B (en) * | 2020-04-20 | 2021-06-08 | 浙江金大康动物保健品有限公司 | Gradient full-information thin-layer identification method for rhizoma atractylodis medicinal material |
| CN112730698A (en) * | 2021-02-03 | 2021-04-30 | 仲景宛西制药股份有限公司 | Content determination method of traditional Chinese medicine preparation for treating depression |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1171264A (en) * | 1997-04-18 | 1998-01-28 | 宋杨 | Calming and desadening pill preparing process thereof |
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| CN1171264A (en) * | 1997-04-18 | 1998-01-28 | 宋杨 | Calming and desadening pill preparing process thereof |
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