CN105628696A - Method for determining content of total phenolic acid in salivae miltiorrhizae liguspyragine hydrochloride and glucose injection - Google Patents
Method for determining content of total phenolic acid in salivae miltiorrhizae liguspyragine hydrochloride and glucose injection Download PDFInfo
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- CN105628696A CN105628696A CN201511008189.XA CN201511008189A CN105628696A CN 105628696 A CN105628696 A CN 105628696A CN 201511008189 A CN201511008189 A CN 201511008189A CN 105628696 A CN105628696 A CN 105628696A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
- G01N2021/3155—Measuring in two spectral ranges, e.g. UV and visible
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Abstract
The invention discloses a determination method for the content of total phenolic acid in a salivae miltiorrhizae liguspyragine hydrochloride and glucose injection. The determination method comprises the following steps of 1 reference solution preparation; 2 test solution preparation; 3 determination. According to the determination method, content determination is performed on the total phenolic acid in the salivae miltiorrhizae liguspyragine hydrochloride and glucose injection through a sodium nitrite color developing method; the determination method is simple and feasible, the determined index is precise, and quality control over drugs can be further improved.
Description
Technical field:
The present invention relates to Chemical Analysis field and pharmaceutical analysis technical field, particularly a kind of method measuring total phenolics content in ginseng rhizome of chuanxiong glucose injection.
Background technology:
Ginseng rhizome of chuanxiong glucose injection is that " Ministry of Health of the People's Republic of China " records kind, does not record relevant total phenolics assay in primary standard, there is obstacle to improving the quality to medicine further.
Summary of the invention:
It is an object of the invention to the deficiency existing for prior art and provide a kind of total phenolics assay in ginseng rhizome of chuanxiong glucose injection that measures, this method measuring method simple possible, testing index is accurate, can further improve the quality control to medicine.
For realizing the object of the present invention, the technical solution adopted in the present invention is:
A kind of method measuring total phenolics content in ginseng rhizome of chuanxiong glucose injection, it is characterised in that, comprise the steps:
1) preparation process of reference substance solution:
Precision takes Sodium Danshensu reference substance 5.0mg, puts in 100mL measuring bottle, is dissolved in water and is diluted to scale, shakes even, obtains the reference substance solution that concentration is 0.05mg/mL;
2) preparation process of need testing solution:
Precision measures ginseng rhizome of chuanxiong glucose injection 1mL, puts in 10mL measuring bottle, is diluted with water to scale, shakes even, obtain need testing solution;
3) determination step:
Precision measures need testing solution 3ml and puts in 10ml volumetric flask, and add water 2mL, then adds 10% sodium nitrite solution 0.5ml, 10% aluminum nitrate solution 1.0ml, shake even after, dark place place 5min, add 2mol/L sodium hydroxide solution and be settled to scale, shake even; Dark place is placed 20min and is namely obtained measurement need testing solution; Precision measures reference substance solution 3ml and puts in 10ml volumetric flask, and add water 2mL, then adds 10% sodium nitrite solution 0.5ml, 10% aluminum nitrate solution 1.0ml, shake even after, dark place place 5min, add 2mol/L sodium hydroxide solution and be settled to scale, shake even; Dark place is placed 20min and is namely obtained measurement reference substance solution; Measure the absorption value measured need testing solution and measure reference substance solution with ultraviolet-visible pectrophotometer at 501nm, calculate total phenolics content in ginseng rhizome of chuanxiong glucose injection.
Total phenolics in ginseng rhizome of chuanxiong glucose injection is carried out assay, this method measuring method simple possible by the present invention's Sodium Nitrite development process, and testing index is accurate, can further improve the quality control to medicine.
Embodiment
Below by the mode of embodiment, the present invention is described further, but the present invention is not limited in the embodiment shown.
Comparative example 1:FoLin-CiocaLteu development process:
1. reference substance solution preparation: precision takes salvianolic acid B reference substance 10mg, puts in 100ml measuring bottle, adds water and makes dissolving and be diluted to scale, shakes even, obtains the reference substance solution of 0.1mg/ml;
2. the preparation of need testing solution: precision measures this product 1.0ml, puts in 10ml volumetric flask, is diluted with water to scale, shakes even, obtains need testing solution;
3. assay method: precision measures need testing solution 1ml, puts in 10 measuring bottles, and add water 2ml, then adds FoLin-CiocaLteu reagent 1.0ml, shaking even, dark place places 5 minutes, then the sodium carbonate solution 2.0ml that adds 7.5%, shake even: dark place places 5 minutes, is diluted with water to scale, obtain measurement need testing solution; Precision measures reference substance solution 1ml, puts in 10 measuring bottles, and add water 2ml, then adds FoLin-CiocaLteu reagent 1.0ml, shaking even, dark place places 5 minutes, then the sodium carbonate solution 2.0ml that adds 7.5%, shake even: dark place places 5 minutes, is diluted with water to scale, obtain measurement reference substance solution; Respectively at 0min, 5min, 10min, 15min, 30min, 45min, 60min, 90min, 120min place, measure measurement need testing solution and measurement need testing solution absorption value with ultraviolet-visible pectrophotometer at 375nm, seek the RSD value of its absorbancy;
The results are shown in Table 1
Table 1
Embodiment 1 Sodium Nitrite development process:
1. the preparation of reference substance solution: precision takes Sodium Danshensu reference substance 5.0mg, puts in 100mL measuring bottle, is dissolved in water and is diluted to scale, shakes even, obtains the reference substance solution that concentration is 0.05mg/mL;
2. the preparation of need testing solution: precision measures this product 1mL, puts in 10mL measuring bottle, is diluted with water to scale, shakes even, obtains need testing solution;
3. assay method: precision measures need testing solution 3ml and puts in 10ml volumetric flask, add water 2mL, then adds 10% sodium nitrite solution 0.5ml, 10% aluminum nitrate solution 1.0ml, shake even after, dark place place 5min, add 2mol/L sodium hydroxide solution and it is settled to scale, shake even; 20min is placed in dark place, obtains measurement need testing solution; Precision measures reference substance solution 3ml and puts in 10ml volumetric flask, and add water 2mL, then adds 10% sodium nitrite solution 0.5ml, 10% aluminum nitrate solution 1.0ml, shake even after, dark place place 5min, add 2mol/L sodium hydroxide solution and be settled to scale, shake even; Dark place is placed 20min and is namely obtained measurement reference substance solution; Respectively at 0min, 5min, 10min, 15min, 30min, 45min, 60min, 90min, 120min place, measure the absorption value measured need testing solution and measure reference substance solution with ultraviolet-visible pectrophotometer at 501nm, seek the RSD value of its absorbancy;
The results are shown in Table 2
Table 2
According to above comparative example it may be seen that the stability of FoLin-CiocaLteu development process is poor. Through test, result shows, FoLin-CiocaLteu determination of color total phenolics, after room temperature places 30 minutes, absorbancy starts to reduce, after room temperature places 2 hours, RSD reaches 9.64%, the compound preparation that ginseng rhizome of chuanxiong glucose injection is made up of ligustrazine hydrochloride and Radix Salviae Miltiorrhizae total phenolic acids extract, and the maximum absorption wavelength of ligustrazine hydrochloride and Radix Salviae Miltiorrhizae total phenolic acids is close, therefore when adopting the content that spectrophotometry directly measures wherein Radix Salviae Miltiorrhizae total phenolic acids, its content will be produced significant impact by ligustrazine hydrochloride; Radix Salviae Miltiorrhizae total phenolic acids can form complex compound with aluminum nitrate and make its maximum absorption wavelength red shift, thus the impact of ligustrazine hydrochloride can also be eliminated, therefore, embodiment 1 Sodium Nitrite development process is selected to be measured by total phenolics, test-results shows, Sodium Nitrite development process is stable good in 2 hours, and RSD is 1.19%. The present invention's Sodium Nitrite determination of color used is convenient. Fast, specificity and favorable reproducibility, result accurately and reliably, can be used for the quality control of said preparation.
Certainly, above-mentioned explanation is not to the restriction of invention, and the present invention is also not limited to above-mentioned citing, those skilled in the art, change, remodeling, interpolation or the replacement made in the essential scope of the present invention, also should belong to protection scope of the present invention.
Claims (1)
1. one kind measures the method for total phenolics content in ginseng rhizome of chuanxiong glucose injection, it is characterised in that, comprise the steps:
1) preparation process of reference substance solution:
Precision takes Sodium Danshensu reference substance 5.0mg, puts in 100mL measuring bottle, is dissolved in water and is diluted to scale, shakes even, obtains the reference substance solution that concentration is 0.05mg/mL;
2) preparation process of need testing solution:
Precision measures ginseng rhizome of chuanxiong glucose injection 1mL, puts in 10mL measuring bottle, is diluted with water to scale, shakes even, obtain need testing solution;
3) determination step:
Precision measures need testing solution 3ml and puts in 10ml volumetric flask, and add water 2mL, then adds 10% sodium nitrite solution 0.5ml, 10% aluminum nitrate solution 1.0ml, shake even after, dark place place 5min, add 2mol/L sodium hydroxide solution and be settled to scale, shake even; Dark place is placed 20min and is namely obtained measurement need testing solution; Precision measures reference substance solution 3ml and puts in 10ml volumetric flask, and add water 2mL, then adds 10% sodium nitrite solution 0.5ml, 10% aluminum nitrate solution 1.0ml, shake even after, dark place place 5min, add 2mol/L sodium hydroxide solution and be settled to scale, shake even; Dark place is placed 20min and is namely obtained measurement reference substance solution; Measure the absorption value measured need testing solution and measure reference substance solution with ultraviolet-visible pectrophotometer at 501nm, calculate total phenolics content in ginseng rhizome of chuanxiong glucose injection.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1338889A2 (en) * | 2002-02-20 | 2003-08-27 | MERCK PATENT GmbH | Method and device for detecting fats and oils |
| CN101129463A (en) * | 2007-08-20 | 2008-02-27 | 正大青春宝药业有限公司 | Method of preparing red sage root injection and quality control method thereof |
| KR20100114320A (en) * | 2009-04-15 | 2010-10-25 | 주식회사 코스메카코리아 | Cosmetic composition for skin whitening containing sodium danshensu |
| CN102621265A (en) * | 2012-03-27 | 2012-08-01 | 贵州景峰注射剂有限公司 | Method for measuring contents of multiple components in Shenxiong glucose injection |
| CN104013673A (en) * | 2013-02-28 | 2014-09-03 | 成都中医药大学 | Salvia extract and preparation method thereof |
-
2015
- 2015-12-28 CN CN201511008189.XA patent/CN105628696A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1338889A2 (en) * | 2002-02-20 | 2003-08-27 | MERCK PATENT GmbH | Method and device for detecting fats and oils |
| CN101129463A (en) * | 2007-08-20 | 2008-02-27 | 正大青春宝药业有限公司 | Method of preparing red sage root injection and quality control method thereof |
| KR20100114320A (en) * | 2009-04-15 | 2010-10-25 | 주식회사 코스메카코리아 | Cosmetic composition for skin whitening containing sodium danshensu |
| CN102621265A (en) * | 2012-03-27 | 2012-08-01 | 贵州景峰注射剂有限公司 | Method for measuring contents of multiple components in Shenxiong glucose injection |
| CN104013673A (en) * | 2013-02-28 | 2014-09-03 | 成都中医药大学 | Salvia extract and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 于晶晶 等: "RP-HPLC同时测定香丹注射液中丹参素钠、原儿茶醛和丹酚酸B的含量", 《海峡药学》 * |
| 朱迪 等: "参芎葡萄糖注射液中总酚酸含量测定方法研究", 《中国药业》 * |
| 李安平 等: "一测多评HPLC法测定丹参注射液中7个水溶性成分含量", 《药物分析杂志》 * |
| 陈焕娜 等: "分光光度法测定丹参总酚含量方法的研究", 《亚太传统医药》 * |
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