CN104316520A - Detection method of trace aluminum in tea polyphenol - Google Patents
Detection method of trace aluminum in tea polyphenol Download PDFInfo
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- CN104316520A CN104316520A CN201410573262.7A CN201410573262A CN104316520A CN 104316520 A CN104316520 A CN 104316520A CN 201410573262 A CN201410573262 A CN 201410573262A CN 104316520 A CN104316520 A CN 104316520A
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Abstract
The invention relates to a detection method of trace aluminum in tea polyphenol, belonging to the field of a food additive and a detection method thereof. The detection method of the trace aluminum in the tea polyphenol comprises the following steps: (1) accurately pipetting 0.5mL, 1.0mL, 2.0mL, 3.0mL, 5.0mL, 10.0mL, 11.0mL, 13.0mL and 15.0mL of 1microgram/milliliter aluminum standard solution into a 50-mL volumetric flask; carrying out developing treatment and determining absorbance at a part with the wavelength of 640nm by a 1-cm cuvette; drawing a curve by the absorbance and the concentration of the aluminum standard solution; and (2) taking 5g of a tea polyphenol test sample to prepare 0.1g/mL solution to be detected; adding 5.0mL of the solution into a 50-mL volumetric flask; after carrying out developing treatment, taking a reagent blank as reference and determining absorbance at the part with the wavelength of 640nm by the 1-cm cuvette; and substituting the absorbance into a standard regression equation; and calculating the content of the aluminum. According to the detection method of the trace aluminum in the tea polyphenol, a spectrophotometry determination method is adopted and is simple, convenient and rapid; the result is accurate, the repeatability is high and the detection limit is low; and the detection method has a high practical value on the content control of the trace aluminum in a production process of the tea polyphenol and in the product, and can be used as a conventional detection method.
Description
Technical field
The present invention relates to a kind of detection method of aluminum in tea polyphenols, belong to food additives and detection method field thereof.
Background technology
Tea Polyphenols is the general name of Catechin in Tea class, flavonoids, phenolic acid class and anthocyan compound, accounts for the 15-25% of dry weight of tea leaves.Tea Polyphenols has free radical in removing, removes superoxide ion, Cell protection film and cell membrane, eliminates the effects such as lipid peroxidation free radical.Tea Polyphenols is antibacterial, bactericidal action in addition, effectively can reduce the absorption of large intestine to cholesterol, prevent and treat atherosclerotic, the effect such as antitumor, radioresistance.Tea Polyphenols is safe, nontoxic, has broad application prospects in all many-sides such as medicine, daily use chemicals, lightization, cosmetics, food, grease, health care.The method preparing Tea Polyphenols from tealeaves mainly divides three classes: solvent extraction method, ion catch electron microscopy and column chromatography preparation method.Ion catch electron microscopy utilizes metal to precipitate Tea Polyphenols, and make it be separated with caffeine, as mantoquita, lead salt or aluminium choride.At present, built and the Tea Polyphenols built of China, whole employing be traditional solvent extraction method and precipitate and separate, the dissolvent residual of product and metal ion content aspect can not meet the requirement of the domestic and international travelling trader day by day improved, especially owing to using aluminium salt as precipitation agent in technique, the residual aluminium ion serious threat mankind's is healthy.At present also not to the assay method of aluminum in tea polyphenols.
Therefore the detection method studying the aluminum in tea polyphenols that a kind of accuracy is high, repeatability is high, easy and simple to handle, detection limit is low is necessary.
Summary of the invention
The present invention aims to provide the detection method of the aluminum in tea polyphenols that a kind of accuracy is high, repeatability is high, easy and simple to handle, detection limit is low.
The detection method of described aluminum in tea polyphenols, comprises the following steps:
(1) accurately pipette 1 μ g/mL aluminum standard solution 0.5,1.0,2.0,3.0,5.0,10.0,11.0,13.0,15.0mL is in 50mL volumetric flask, after color development treatment, absorbance is measured in wavelength 640nm place, with the concentration curve plotting of absorbance and aluminum standard solution with 1cm cuvette.
(2) get Tea Polyphenols test specimens 5g, be made into 0.1g/mL liquid to be measured, get 5.0mL in 50mL volumetric flask, after color development treatment, make reference with reagent blank, measure absorbance with 1cm cuvette in wavelength 640nm place, absorbance is substituted into standard regressive method, calculates the content of aluminium.
Preferably, color development treatment method of the present invention is in the volumetric flask that a certain amount of aluminum standard solution or test specimens are housed, add water to 20mL, add 0.5g/L ascorbic acid solution 1mL more successively, 1g/L chrome azurol S reagent 3.0mL, acetic acid-ammonium acetate buffer solution 15mL, is diluted with water to scale, shake up, place 10min.
In detection method of the present invention, after measured, the absorbance of aluminum standard solution and the concentration curve plotting of aluminum standard solution are
Y=0.1.267+0.0225X, coefficient R=0.9996, RSD=0.2%, this method linear relationship is good, and recovery of standard addition, between 95.15-106.12%, shows that this method accuracy is higher, can be used for the quantitative measurement of aluminium content in Tea Polyphenols.
The detection method of aluminum in tea polyphenols of the present invention, adopt spectrophotometry, method is easy, quick, result is accurate, the repeatability high detection limit is low, content for Trace Aluminum in Tea Polyphenols production run and product controls there is higher practical value, can be used as common detection methods application.
Embodiment
Embodiment one:
(1) accurately pipette 1 μ g/mL aluminum standard solution 0.5,1.0,2.0,3.0,5.0,10.0,11.0,13.0,15.0mL is in 50mL volumetric flask, after color development treatment, absorbance is measured in wavelength 640nm place, with the concentration curve plotting of absorbance and aluminum standard solution with 1cm cuvette.After measured, the absorbance of aluminum standard solution and the concentration curve plotting of aluminum standard solution are
Y=0.1.267+0.0225X, coefficient R=0.9996, RSD=0.2%.
(2) get Tea Polyphenols test specimens 5g, be made into 0.1g/mL liquid to be measured, get 5.0mL in 50mL volumetric flask, after color development treatment, make reference with reagent blank, measure absorbance with 1cm cuvette in wavelength 640nm place, absorbance is substituted into standard regressive method, calculates the content of aluminium.
Following data carry out Trace Aluminum detection to commercially available two kinds of Tea Polyphenols products (one is crude product, and one is crude product), and its testing result is as follows:
。
Claims (2)
1. the detection method of aluminum in tea polyphenols, comprises the following steps:
(1) accurately pipette 1 μ g/mL aluminum standard solution 0.5,1.0,2.0,3.0,5.0,10.0,11.0,13.0,15.0mL is in 50mL volumetric flask, after color development treatment, absorbance is measured in wavelength 640nm place, with the concentration curve plotting of absorbance and aluminum standard solution with 1cm cuvette;
(2) get Tea Polyphenols test specimens 5g, be made into 0.1g/mL liquid to be measured, get 5.0mL in 50mL volumetric flask, after color development treatment, make reference with reagent blank, measure absorbance with 1cm cuvette in wavelength 640nm place, absorbance is substituted into standard regressive method, calculates the content of aluminium.
2. the detection method of aluminum in tea polyphenols as claimed in claim 1, it is characterized in that described color development treatment method is in the volumetric flask that a certain amount of aluminum standard solution or test specimens are housed, add water to 20mL, add 0.5g/L ascorbic acid solution 1mL more successively, 1g/L chrome azurol S reagent 3.0mL, acetic acid-ammonium acetate buffer solution 15mL, is diluted with water to scale, shake up, place 10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410573262.7A CN104316520A (en) | 2014-10-23 | 2014-10-23 | Detection method of trace aluminum in tea polyphenol |
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| CN201410573262.7A CN104316520A (en) | 2014-10-23 | 2014-10-23 | Detection method of trace aluminum in tea polyphenol |
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| CN104316520A true CN104316520A (en) | 2015-01-28 |
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| CN201410573262.7A Pending CN104316520A (en) | 2014-10-23 | 2014-10-23 | Detection method of trace aluminum in tea polyphenol |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104990916A (en) * | 2015-06-30 | 2015-10-21 | 贵州黄平靓鸥桑综合开发有限公司 | Identifier for food additive |
| CN105300962A (en) * | 2015-02-05 | 2016-02-03 | 温州医科大学 | Water aluminum ion on-site quick detecting method capable of eliminating reagent blank impacts |
-
2014
- 2014-10-23 CN CN201410573262.7A patent/CN104316520A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300962A (en) * | 2015-02-05 | 2016-02-03 | 温州医科大学 | Water aluminum ion on-site quick detecting method capable of eliminating reagent blank impacts |
| CN105300962B (en) * | 2015-02-05 | 2018-04-24 | 温州医科大学 | Aluminium ion field fast detection method in a kind of water for eliminating reagent blank influence |
| CN104990916A (en) * | 2015-06-30 | 2015-10-21 | 贵州黄平靓鸥桑综合开发有限公司 | Identifier for food additive |
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Application publication date: 20150128 |