CN105624216A - Process for biosynthesis of high-purity resveratrol - Google Patents

Abstract

The invention discloses a process for biosynthesis of high-purity resveratrol. The process is simple, low in production cost and easy for industrial production. The process comprises the following steps: firstly, inoculating an aspergillus aculeatus strain to a fermentation medium consisting of grape skin, rhizoma polygoni cuspidati and peanut shell powder, so that a fermentation broth is formed; then, centrifuging the fermentation broth in a high-speed centrifuge so as to form clear liquid; adding ethanol to the clear liquid, keeping for 9h, centrifuging and reserving a supernatant; conducting vacuum rotary evaporation and concentration on the supernatant, and recovering the ethanol; and freeze-drying a concentrated solution, so that a resveratrol sample is obtained, wherein the resveratrol sample is 98.1% in purity to the greatest extent.

Description

The technique of biosynthesis high-purity resveratrol

Technical field

The invention belongs to biological technical field, the technique being specifically related to a kind of biosynthesis high-purity resveratrol.

Background technology

The technological process of biosynthesis high-purity resveratrol at least includes: the basic processes such as centrifugal, dry, freezing and concentration purification. In the technique of current biosynthesis high-purity resveratrol, the product cost made is too high, and environmental pollution is serious, and not only manufacturing process is complicated, and production efficiency is low, and the holding time is short.

Summary of the invention

For the above-mentioned technical problem overcoming prior art to exist, it is an object of the invention to, the technique providing a kind of biosynthesis high-purity resveratrol, the present invention not only manufacturing process is simple, production efficiency is higher, and product mouth quality is good, the holding time is longer.

The technique of biosynthesis high-purity resveratrol provided by the invention, comprises the following steps:

(1) microorganism Aspergillus aculeatus strain is inoculated in the fermentation medium being made up of Pericarpium Vitis viniferae, Rhizoma Polygoni Cuspidati and peanut hull meal, cultivates 3-5 days for 40 DEG C-60 DEG C, form fermentation liquid;

(2) fermentation liquid is introduced in high speed centrifuge and centrifugal to form clear liquid, take clear liquid and add after ethanol after static 9-12 hour, centrifuging and taking supernatant;

(3) supernatant is concentrated through rotary evaporation in vacuo, reclaim ethanol, by concentrated solution lyophilization, obtain resveratrol sample.

The technique of biosynthesis high-purity resveratrol provided by the invention, it has the beneficial effects that, overcomes operation in prior art processing resveratrol technique more, and the problem that workload is big improves production efficiency; Avoiding the mass defect caused because product yield is low, utilization rate of raw materials is high, reduces production cost; It is effectively ensured product quality.

Detailed description of the invention

Below in conjunction with an embodiment, the technique of biosynthesis high-purity resveratrol provided by the invention is described in detail.

Embodiment

The technique of the biosynthesis high-purity resveratrol of the present embodiment, comprises the following steps:

(1) microorganism Aspergillus aculeatus strain is inoculated in the fermentation medium being made up of Pericarpium Vitis viniferae, Rhizoma Polygoni Cuspidati and peanut hull meal, cultivates 5 days for 60 DEG C, form fermentation liquid;

(2) fermentation liquid is introduced in high speed centrifuge and centrifugal to form clear liquid, take clear liquid and add after ethanol after static 9 hours, centrifuging and taking supernatant;

(3) supernatant is concentrated through rotary evaporation in vacuo, reclaim ethanol, by concentrated solution lyophilization, obtain resveratrol sample.

The technique of biosynthesis high-purity resveratrol, can directly produce high-purity resveratrol, it is not necessary to be processed further, and operation is simple, produces product yield height, and quality is good, and the holding time is longer.

Claims (1)

1. the technique of a biosynthesis high-purity resveratrol, it is characterised in that: said method comprising the steps of:

(1) microorganism Aspergillus aculeatus strain is inoculated in the fermentation medium being made up of Pericarpium Vitis viniferae, Rhizoma Polygoni Cuspidati and peanut hull meal, cultivates 3-5 days for 40 DEG C-60 DEG C, form fermentation liquid;

(2) fermentation liquid is introduced in high speed centrifuge and centrifugal to form clear liquid, take clear liquid and add after ethanol after static 9-12 hour, centrifuging and taking supernatant;

(3) supernatant is concentrated through rotary evaporation in vacuo, reclaim ethanol, by concentrated solution lyophilization, obtain resveratrol sample.