CN104388475A - Method for recycling yeast abandoned after erythritol production - Google Patents
Method for recycling yeast abandoned after erythritol production Download PDFInfo
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- CN104388475A CN104388475A CN201410675566.4A CN201410675566A CN104388475A CN 104388475 A CN104388475 A CN 104388475A CN 201410675566 A CN201410675566 A CN 201410675566A CN 104388475 A CN104388475 A CN 104388475A
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Abstract
The invention discloses a method for recycling yeast abandoned after erythritol production. According to the method, bacterial cells separated out from an erythritol fermentation liquor are autolyzed under assistance, the autolysate is concentrated and used as an erythritol fermentation accessory instead of commercial yeast extract or yeast cream, the bacterial cell residue left after the autolysate is separated out is treated by use of an alkali liquor to obtain yeast cell wall polysaccharides, and the used alkali liquor is applied to regulating the pH in the erythritol fermentation process. The method for recycling the yeast abandoned after the erythritol production realizes complete utilization of the abandoned yeast cells in the erythritol production without causing new pollution to the process, and is especially suitable for popularization to erythritol production enterprises.
Description
Technical field
The present invention relates to a kind of method for waste yeast thalline recycling in erythritol production, belong to biological chemical field.
Background technology
Erythritol is a kind of novel glycitols sweetening agent, has wide application at production fields such as functional food, beverage, Special foods; It is produced at present is mainly that raw material adopts osmophilic yeast to carry out microbe conversion with glucose, and the fermented liquid obtained is separated through thalline, purification and impurity removal, and then crystallization, recrystallization obtain erythritol product.But during erythritol is produced, waste yeast thalline becomes one of maximum by product of output (or waste), often produce the yeast wet thallus that 1 ton of large appointment of erythritol produces 500kg.Yeast thalline moisture content after centrifugation is 75-85%, not easy to store, if can not process in time, will be putrid and deteriorated, and not only increase cost of disposal, also can increase carrying capacity of environment simultaneously.Current manufacturing enterprise is generally by the wet thallus after being separated directly at a low price or freely process to neighbouring feed manufacturing emterprise, not only cannot bring higher economic benefit to enterprise, there is larger risk of environmental pollution on the contrary.Along with development and the production-scale expansion of erythritol industry, the output of fermented abandoned yeast thalline also will constantly increase, if can not dispose its effectively appropriate processing, will increase the economy and environment burden of enterprise, hinder the sound development of enterprise.Contriver is by finding after waste yeast Principle components analysis in erythritol production, in cell, crude protein content only has about 25%, polysaccharide content is then up to more than 55%, its protein content is obviously on the low side compared with bread yeast or cereuisiae fermentum, and polysaccharide content is obviously higher, there is higher utility value.Based on this, research and develop a kind of technological method for erythritol waste yeast thalline recycling on the spot significant.
Retrieval finds that the R&D direction of relevant erythritol production mainly concentrates on the control aspect of productive rate and the fermentation condition how improving bacterial strain erythritol, and utilizes the rare report of the research of aspect for byproduct comprehensives such as the waste yeast thalline produced in production.
Summary of the invention
Utilize the deficiency of technology for the fermented abandoned yeast thalline of erythritol, the present invention proposes a kind of method for waste yeast thalline recycling in erythritol production.
Method for waste yeast thalline recycling in erythritol production of the present invention, step is:
(1) erythritol is produced the centrifugal yeast somatocyte mud obtained of fermented liquid thalline separation circuit, add water, be mixed with weight/volume (W/V, g/100mL) meter the somatic cells suspension that concentration is 10-15%; Or the yeast somatocyte suspension that thalline separation circuit ceramic membrane filter obtained, be adjusted to and count with weight/volume (W/V, g/100mL) the somatic cells suspension that concentration is 10-15%; For subsequent use;
(2) with weight/volume (W/V, g/100mL) the solid NaCl adding 1-2% in the somatic cells suspension obtained to step (1) is counted, abundant stirring and dissolving, mix, then carry out once high-pressure homogeneous under pressure 45-55MPa, in homogenizing process, keep feed temperature to be no more than 50 DEG C;
(3) feed liquid after homogeneous is incubated self-dissolving 10-24 hour at 50 ± 2 DEG C, then centrifugal with 3000-5000 rev/min, obtain somatic cells self-dissolving clear liquid and self-dissolving residue respectively;
(4) somatic cells self-dissolving clear liquid step (3) obtained under 60-70 DEG C of condition vacuum concentration to solid content with weight/volume (W/V, g/100mL) count 65-75%, namely obtain somatic cells self-dissolving clear liquid enriched material.This enriched material substitute goods yeast extractive substance is used for erythritol fermentation batches;
(5) the somatic cells self-dissolving residue that step (3) obtains is suspended in that the NaOH solution that concentration is 0.1-0.2mol/L or step (6) centrifugation obtain containing in alkali supernatant liquor, regulate self-dissolving sludge concentration with weight/volume (W/V, g/100mL) 10-15% is counted, extracting 1.0-2.0 hour is stirred under putting 90-100 DEG C of condition, then centrifugal with 3000-5000 rev/min, obtain containing alkali supernatant liquor and throw out respectively; Be used for containing alkali supernatant liquor the adjustment that erythritol produces fermentation pH, throw out continues extracting;
(6) throw out settling flux step (5) obtained is in the NaOH solution of 0.1-0.2mol/L in concentration, and regulate sludge concentration with weight/volume (W/V, g/100mL) 10-15% is counted, extracting 0.5-1.0 hour is stirred under putting 90-100 DEG C of condition, then centrifugal with 3000-5000 rev/min, then obtain containing alkali supernatant liquor and throw out respectively; Be used for suspension and the alkaline purification of somatic cells self-dissolving residue in step (5) containing alkali supernatant liquor, throw out continues process;
(7) throw out that step (6) obtains is added water washing 2-3 time, again with 3000-5000 rev/min of centrifugal collecting precipitate, or adopt vacuum filtration, collect solid, then vacuum lyophilization or lyophilize are implemented to it, obtain zymosan product.
In the above-mentioned method for waste yeast thalline recycling in erythritol production: the preferred 48-52MPa of the described high-pressure homogeneous pressure of step (2).
In the above-mentioned method for waste yeast thalline recycling in erythritol production: step (3) the described self-dissolving time is preferably 15-20 hour.
In the above-mentioned method for waste yeast thalline recycling in erythritol production: the concentration of step (5) or (6) described NaOH solution is preferably 0.15-0.2mol/L.
The method recycled for waste yeast thalline in erythritol production of the present invention has the following advantages and beneficial effect:
The present invention, in conjunction with the feature of the existing production technique of erythritol and erythritol fermented yeast somatic cells self, proposes a kind of erythritol and produces waste yeast thalline site disposal resource utilization scheme.Existing thalline separating technology in producing according to erythritol, by the discarded thalline produced in production process, directly processes after suitably regulating moisture, does not need to collect separately, deposit, greatly reduce processing cost and risk of environmental pollution.
The present invention proposes erythritol and produces waste yeast thalline site disposal resource utilization scheme, is concentrated by the autolyzate clear liquid that the self-dissolving of waste yeast thalline produces, and enriched material substitute goods yeast extract or yeast extract paste are used for erythritol and produce fermentation batches.Prove through fermenting experiment, this extract completely can substitute goods yeast extract or yeast extract paste, and erythritol fermentation period and erythritol yield are not all affected, and saves the raw materials cost that erythritol is produced.
The erythritol that the present invention proposes produces waste yeast site disposal resource utilization scheme, the alkali lye produced in zymosan leaching process is used for the adjustment that erythritol produces fermenting process pH, through fermentation test checking, erythritol fermenting process is not affected, the commodity alkali lye (NaOH) used now can be substituted completely, not only reduce the raw materials cost that erythritol is produced, avoid the problem of environmental pollution that the outer scheduling of alkali lye is raw simultaneously.
The present invention is in conjunction with the feature of the existing production technique of erythritol and erythritol fermented yeast somatic cells self, propose a kind of erythritol and produce waste yeast thalline site disposal resource utilization scheme, first by somatic cells self-dissolving, after being separated autolyzate, remaining self-dissolving residue produces cell wall polysaccharides by alkaline purification.Contriver finds by producing the composition analysis of yeast somatocyte to erythritol, compared with bread yeast, cereuisiae fermentum, it is relatively low that erythritol produces yeast protein content, and polysaccharide content is higher, therefore extraction polysaccharide has clear superiority, high for Polyose extraction yield, purity is high, and added value is large.
The present invention is in conjunction with the feature of the existing production technique of erythritol and erythritol fermented yeast self, propose a kind of erythritol and produce waste yeast thalline site disposal resource utilization scheme, first the thalline that fermentation is separated is assisted self-dissolving, after the autolyzate obtained is concentrated, substitute goods yeast extract or yeast extract paste are used for erythritol fermentation batches, the yeast residue base extraction be separated after autolyzate produces yeast cells wall polysaccharide, alkali lye after process is used for erythritol fermenting process pH regulator, somatic cells utilizes completely, and does not have new pollutent to produce.
The present invention proposes erythritol and produces waste yeast thalline resource utilization scheme, combines the feature of the existing production technique of erythritol and erythritol fermented yeast somatic cells self, with strong points, is particularly suitable for promoting in erythritol manufacturing enterprise.
Embodiment
Embodiment 1:
(1) erythritol is produced the centrifugal yeast somatocyte bacterium mud obtained of fermented liquid thalline separation circuit, add water, be mixed with in g/100mL the suspension that barm cell concentration is 13%; Or the yeast somatocyte suspension that thalline separation circuit ceramic membrane filter obtained, being adjusted in g/100mL cell concentration is the suspension of 13%; For subsequent use;
(2) in the obtained yeast suspension of step (1), add the solid NaCl of 1.6% in g/100mL, be fully uniformly mixed, then carry out once high-pressure homogeneous under pressure 50MPa, in homogenizing process, keep feed temperature to be no more than 50 DEG C;
(3) feed liquid after homogeneous is incubated self-dissolvings 20 hours at 50 ± 2 DEG C, then centrifugal with 4500 revs/min, obtain somatic cells self-dissolving clear liquid and self-dissolving residue respectively;
(4) the somatic cells self-dissolving clear liquid vacuum concentration under 65 DEG C of conditions step (3) obtained counts 70% to solid content with g/100mL, namely somatic cells self-dissolving clear liquid enriched material is obtained, for the commodity yeast extractive substance in alternative erythritol fermentation batches;
(5) the somatic cells self-dissolving residue that step (3) obtains is suspended in that the NaOH solution that concentration is 0.16mol/L or step (6) centrifugation obtain containing in alkali supernatant liquor, yeast autolysis sludge concentration is regulated to count 13% with g/100mL, extracting 2.0 hours is stirred under putting 96 DEG C of conditions, then centrifugal with 4500 revs/min, obtain containing alkali supernatant liquor and throw out respectively; Be used for containing alkali supernatant liquor the adjustment that erythritol produces fermentation pH, throw out continues extracting;
(6) throw out settling flux step (5) obtained is in the NaOH solution of 0.16mol/L in concentration, and regulate sludge concentration to count 13% with g/100mL, extracting 1.0 hours is stirred under putting 96 DEG C of conditions, then centrifugal with 4500 revs/min, then obtain containing alkali supernatant liquor and throw out respectively; Be used for suspension to somatic cells self-dissolving residue in step (5) and alkaline purification containing alkali supernatant liquor, throw out continues process;
(7) throw out that step (6) obtains is added water washing 3 times, again with 4500 revs/min of centrifugal collecting precipitates, or vacuum filtration is adopted to carry out solid-liquid separation, collecting precipitation thing, then vacuum lyophilization or lyophilize are implemented to it, obtain zymosan product.
Embodiment 2
Get erythritol and produce ceramic membrane filter interception liquid (the somatic cells dry concentration 12w/v%) 50L produced in the sepn process of fermented liquid thalline, add 1kg solid NaCl wherein, the laggard horizontal high voltage homogeneous of stirring and dissolving, homogenization pressure 45MPa, homogenizing temperature 50 DEG C, cell suspension after homogeneous in 50 DEG C of insulations, stir self-dissolving 16 hours, centrifugally obtain self-dissolving supernatant liquor and self-dissolving residue respectively.The self-dissolving supernatant liquor obtained is carried out vacuum concentration at 60-65 DEG C, to solid substance 70%, obtains enriched material 5.05kg (containing 1kgNaCl).The self-dissolving residue that centrifugation obtains adds 25L 0.2mol/L Fresh NaOH solution, 100 DEG C of conditions stir 1.5 hours, centrifugation, clear liquid is collected separately (for erythritol fermenting process pH regulator), precipitation adds 25L 0.2mol/L Fresh NaOH solution again, 100 DEG C of conditions stir 0.5 hour, centrifugation, clear liquid is collected separately (the first time base extraction for next batch self-dissolving residue), precipitation adds water washing 2-3 time, centrifugation or vacuum filtration, the solid part collected adopts freeze-drying and dehydrating, obtain zymosan product 1.7kg, 96.1% is about through initial analysis glucan content.
Embodiment 3
Get erythritol and produce ceramic membrane filter interception liquid (the yeast cell dry concentration 12w/v%) 60L produced in fermented liquid sterilizing process, add 1kg solid NaCl wherein, the laggard horizontal high voltage homogeneous of stirring and dissolving, homogenization pressure 50MPa, homogenizing temperature 50 DEG C, cell suspension after homogeneous 50 DEG C insulation, stir self-dissolving 10 hours, centrifugally obtain self-dissolving supernatant liquor and self-dissolving residue respectively.The yeast autolysis suspension obtained is carried out 60-65 DEG C of vacuum concentration to solid substance 70%, obtain concentrated yeast autolyzate 5.25kg (containing 1kgNaCl).The autolysing yeast residue that centrifugation obtains adds 25LNaOH solution (alkaline purification of yeast autolysis residue secondary is separated alkali lye), 95 DEG C of conditions stir 2.0 hours, centrifugation, clear liquid is collected separately (for erythritol fermenting process pH regulator), precipitation adds 25L 0.2mol/L Fresh NaOH solution again, 100 DEG C of conditions stir 1.0 hours, centrifugation, clear liquid is collected separately (the first time base extraction for next batch self-dissolving residue), precipitation adds water washing 2-3 time, centrifugation, the solid part collected adopts freeze-drying and dehydrating, obtain zymosan product 2.1kg, 95.5% is about through initial analysis glucan content.
Embodiment 4
The centrifugal yeast slurry 5kg (moisture 75%) obtained of fermented erythritol liquor, add water and carry out washing once, centrifugation, collect somatic cells mud, cell mud is added 8L water, be made into the somatic cells suspension that massfraction is about 15%, add 150g solid NaCl wherein, the laggard horizontal high voltage homogeneous of stirring and dissolving, homogenization pressure 55MPa, homogenizing temperature 50 DEG C, the cell suspension after homogeneous in 50 DEG C of insulations, stir self-dissolving 18 hours, centrifugally obtain self-dissolving supernatant liquor and self-dissolving residue respectively.The self-dissolving suspension obtained is carried out 60-65 DEG C of vacuum concentration to solid substance 70%, obtain concentrated autolyzate 920g (containing 0.15kgNaCl).The autolysing yeast residue that centrifugation obtains adds 7.5L 0.2mol/L Fresh NaOH solution, 100 DEG C of conditions stir 1.5 hours, centrifugation, clear liquid is collected separately, precipitation adds 7.5L 0.2mol/L Fresh NaOH solution again, 100 DEG C of conditions stir 0.5 hour, centrifugation, clear liquid is collected separately (the first time base extraction for next batch self-dissolving residue), precipitation adds water washing 2-3 time, vacuum filtration, and the solid part of collection adopts freeze-drying and dehydrating, obtain zymosan product 250g, be about 96.6% through initial analysis glucan content.
Embodiment 5
Concentrated autolyzate substitute goods yeast extract and yeast extract paste are used for erythritol fermentation.
The concentrated autolyzate (addition through optimize) using the inventive method to utilize fermented erythritol liquor separated yeast to prepare and current erythritol produce in the commodity yeast extractive substance of employing and yeast extract paste (the suitableeest addition) do and erythritol shake flask fermentation carried out to contrast and the fermentation test of 10-100L mechanical agitator tank the results are shown in following table 1 and table 2.
Table 1 erythritol shake flask fermentation test-results
Fermentation results shows, the yeast autolysis extract using the inventive method to prepare carries out erythritol fermentation, the commodity yeast extractive substance that fermentation production rate and fermentation period adopt in producing with current erythritol, yeast extract paste result are similar, illustrate that yeast cell self-dissolving extract that the present invention utilizes the fermented abandoned yeast body of erythritol to prepare can substitute goods yeast extractive substance or yeast extract paste be produced for erythritol.
Table 2 10-100L fermentor tank amplification test
Embodiment 6
The alkali lye that process yeast autolysis residue of the present invention is separated is used for erythritol fermenting process pH and controls.Experiment adopts 10L mechanical agitator tank to carry out erythritol fermentation, the alkali lye that fermenting process adopts fresh sodium hydroxide solution and extraction zymosan to reclaim respectively controls fermented liquid pH, experimental result is in table 3, result display is reclaimed alkali lye and is controlled for erythritol fermenting process pH, does not affect erythritol fermenting process and result.
Table 3:
Claims (4)
1. a method for waste yeast thalline recycling in producing for erythritol, step is:
(1) erythritol is produced the centrifugal yeast somatocyte bacterium mud obtained of fermented liquid thalline separation circuit, add water, be mixed with the suspension of cell concentration 10-15% in g/100mL; Or the yeast somatocyte suspension that thalline separation circuit ceramic membrane filter obtained, add water, be deployed in g/100mL the suspension that cell concentration is 10-15%; For subsequent use;
(2) in the obtained somatic cells suspension of step (1), the solid NaCl of 1-2% is added in g/100mL, abundant stirring and dissolving, mix, then carry out once high-pressure homogeneous under pressure 45-55MPa, in homogenizing process, keep feed temperature to be no more than 50 DEG C;
(3) feed liquid after homogeneous is incubated self-dissolving 10-24 hour at 50 ± 2 DEG C, then centrifugal with 3000-5000 rev/min, obtain somatic cells self-dissolving clear liquid and self-dissolving residue respectively;
(4) the somatic cells self-dissolving clear liquid vacuum concentration under 60-70 DEG C of condition step (3) obtained counts 65-75% to solid content with g/100mL, namely somatic cells self-dissolving clear liquid enriched material is obtained, for the commodity yeast extractive substance in alternative erythritol fermentation batches;
(5) the somatic cells self-dissolving residue that step (3) obtains is suspended in that the NaOH solution that concentration is 0.1-0.2mol/L or step (6) centrifugation obtain containing in alkali supernatant liquor, self-dissolving sludge concentration is regulated to count 10-15% with g/100mL, extracting 1.0-2.0 hour is stirred under putting 90-100 DEG C of condition, then centrifugal with 3000-5000 rev/min, obtain containing alkali supernatant liquor and throw out respectively; Be used for containing alkali supernatant liquor the adjustment that erythritol produces fermentation pH, throw out continues extracting;
(6) throw out settling flux step (5) obtained is in the NaOH solution of 0.1-0.2mol/L in concentration, and regulate sludge concentration to count 10-15% with g/100mL, extracting 0.5-1.0 hour is stirred under putting 90-100 DEG C of condition, then centrifugal with 3000-5000 rev/min, then obtain containing alkali supernatant liquor and throw out respectively; Be used for suspension to somatic cells self-dissolving residue in step (5) and alkaline purification containing alkali supernatant liquor, throw out continues process;
(7) throw out that step (6) obtains is added water washing 2-3 time, again with 3000-5000 rev/min of centrifugal collecting precipitate, or adopt vacuum filtration to collect solid, then vacuum lyophilization or lyophilize are implemented to it, obtain zymosan product.
2. in producing for erythritol according to claim 1, the method for waste yeast thalline recycling, is characterized in that: the described high-pressure homogeneous pressure 48-52MPa of step (2).
3. in producing for erythritol according to claim 1, the method for waste yeast thalline recycling, is characterized in that: step (3) the described self-dissolving time is 15-20 hour.
4. in producing for erythritol according to claim 1, the method for waste yeast thalline recycling, is characterized in that: the concentration of step (5) or (6) described NaOH solution is 0.15-0.2mol/L.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104826366A (en) * | 2015-05-27 | 2015-08-12 | 山东福田药业有限公司 | Treatment method for erythritol fungi residues |
| CN104894095A (en) * | 2015-05-19 | 2015-09-09 | 广西科学院生物研究所 | A method of producing erythritol by utilization of waste erythritol bacterial sludge |
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| CN104894095A (en) * | 2015-05-19 | 2015-09-09 | 广西科学院生物研究所 | A method of producing erythritol by utilization of waste erythritol bacterial sludge |
| CN104826366A (en) * | 2015-05-27 | 2015-08-12 | 山东福田药业有限公司 | Treatment method for erythritol fungi residues |
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