CN105624046B - It is a kind of to prevent and treat mycotic black fungus bacterial and its application - Google Patents
It is a kind of to prevent and treat mycotic black fungus bacterial and its application Download PDFInfo
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- CN105624046B CN105624046B CN201610050570.0A CN201610050570A CN105624046B CN 105624046 B CN105624046 B CN 105624046B CN 201610050570 A CN201610050570 A CN 201610050570A CN 105624046 B CN105624046 B CN 105624046B
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- tomato
- gray mold
- botrytis cinerea
- black fungus
- bacterial strain
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
The invention discloses a kind of mycotic black fungus bacterials of prevention and treatment and its application, the black fungus bacterial to beAuricularia auricula‑judae DK6；Deposit number: CCTCC NO:M 2016054.Using the fermentation liquid of the bacterium, the generation and sprawling of gray mold can be effectively inhibited, in excised leaf test, DK6 bacterial strain fermentation liquor, up to 80%, can effectively prevent the extension of scab to the preventive effect of gray mold；Indoor pot experiment result table shows that the scab of DK6 processing group is smaller, and disease index is smaller, and preventive effect is up to 57%.Romaine lettuce gray mold field efficacy (stable disease phase), DK6 fermentation liquor treatment group is up to 73% to the preventive effect of gray mold；And the tunning of the bacterium can also efficiently inhibit the sprouting of botrytis cinerea spore, therefore the bacterial strain is the gray mold biocontrol microorganisms of one plant of great exploitation potential.
Description
Technical field
The invention belongs to technical field of plant disease biological control, and in particular to a kind of mycotic black fungus bacterial of prevention and treatment and
It is applied.
Background technique
Vegetables are one of essential food in people's diet.Vegetables can provide a variety of dimension lifes necessary to human body
The nutriments such as element and minerals.Gray mold caused by the pathogen of Botrytis cinerea (Botrytis cinerea Pers.) is a kind of world
Property fungal disease, which has generally occurred throughout the country, and in rising trend, becomes the important disease in current vegetables production
Evil.Ash arrhizus bacteria host range is very extensive, can infect tomato, romaine lettuce, eggplant, cucumber, capsicum, strawberry, grape, garlic and mandarin orange
400 various crop such as tangerine generates harm to various crops such as vegetables, fruit tree and flowers, especially prominent in the production of protecting field fruits and vegetables
Out.Gray mold occurred and spreads in China since the 1980s, and spread speed is fast, had pole to the production of China vegetables
Big threat, when serious can the underproduction 40%~50%, or even total crop failure.
Gray mold has become a kind of important intractable disease in production, and the generation and sprawling for controlling the disease are to ensure that
Crops production and quality and urgent problem.The prevention and treatment of gray mold at present mainly has following 3 kinds of methods, i.e., bionomic control,
Chemical prevention and biological control.
The crop varieties of cultivation botrytis resistant are a kind of agricultural measures that important control gray mold disease occurs, but so far
Until there has been no the botrytis resistant crop varieties for the application that can put into production on the market.Due to the gray mold resistance money reported at present
Source is rare, and most of complicated quantitative character for controlled by multiple genes, and the breeding for disease resistance work of gray mold is made slow progress always.
For a long time, the prevention and control of plant diseases, pest control of China crops relies primarily on chemical pesticide, and pollution by pesticides is more serious, food
Middle agricultural chemical recall rate is up to 90% or more, this has seriously endangered the health of consumer.Simultaneously as food pesticide residue is asked
Topic leads to agricultural products in China outlet obstructions, this constitutes the development of China's modern agriculture industry and seriously affects.Therefore, pesticide is residual
It stays and has become urgent problem in food safety production.Compared to chemical pesticide, biological pesticide has safe and environment-friendly and without residual
The advantages that staying.Focus on biological pesticide exploitation less toxic, efficient and that selectivity is strong and has become one of current research hotspot.
Currently, many researchers advocate and develop and use biological prevention in primary study to control grey mold disease.It is raw
Object control measure was both environmentally friendly, to safety of human and livestock, and can be effectively controlled the generation of disease with sprawling so quite being favored.Mesh
The preceding ash arrhizus bacteria antagonistic bacterium reported focuses primarily upon bacillus (Bacillus spp.), streptomycete
(Streptomyces spp.), pseudomonad (Pseudomonas spp.) etc., it is true to the preferable biological and ecological methods to prevent plant disease, pests, and erosion of gray mold biocontrol effect
Bacterium mainly has trichoderma (Trichoderma spp.), Gliocladium roseum (Gliocladium roseum) and saccharomycete
(Yeasts).Bruce Lee etc. has found the tunning of Paecilomyces lilacinus (Paecilomyces lilacinus) to the pathogen of Botrytis cinerea
There is bacteriostasis, and there are certain growth-promoting effects to tomato plant, are a kind of fungi biological and ecological methods to prevent plant disease, pests, and erosions of new prevention and treatment graw mold of tomato
The factor.
So far less as the report of biocontrol microorganisms about Filamentous basidiomycetes, the discovery Coriolus such as Liu's mark can degrade chrysanthemum
Powder；Li Guohong has found that basidiomycetes YL 14 has eelworm-killing activity for the first time；The viable bacteria silk of Xiao Yi isogonism basidiomycetes B6 can improve soil
Environment improves the resistance of watermelon plant, and then increases yield.It is less as the report of biocontrol microorganisms in relation to Filamentous basidiomycetes, it uses
It is prevented and treated gray mold and is not occurred any report temporarily.
Summary of the invention
The first purpose of the invention is to provide a kind of black fungus bacterial DK6 (present invention or be DK6 bacterial strain), the bacterial strains pair
Botrytis cinerea has stronger control efficiency.The bacterial strain is sent on January 18th, 2016 to China typical culture collection center preservation,
Classification naming: Auricularia auricula-judae DK6；Deposit number: CCTCC NO:M 2016054；Address: China
Wuhan Wuhan University.
Second object of the present invention is to be the provision of a kind of black fungus bacterial DK6 to treat or prevent fungi diease occurrence in preparation
Application in drug medicine, the prevention and treatment particularly suitable for plant botrytis.
The present invention the last one be designed to provide a kind of black fungus bacterial DK6 and promoting the application in tomato growth.
In order to achieve the above object, the present invention uses following technical measures:
A kind of black fungus bacterial DK6 is obtained in the following manner:
Applicant separates from Chinese Shiyan City, Hubei Province maize leaf, screen obtain one plant have to gray mold it is relatively strong anti-
The bacterial strain for controlling effect, is named as DK6.By the identification of morphology and molecular biology, it is accredited as basidiomycetes Auricularia
(Auricularia spp.).The bacterial strain has been sent on January 18th, 2016 to China typical culture collection center preservation, classification
Name: Auricularia auricula-judae DK6；Deposit number: CCTCC NO:M 2016054；Address: Wuhan, China
Wuhan University.
DK6 bacterial strain colony characteristics: white is integrally presented in bacterium colony.With the growth of incubation time, there is difference in positive and negative color,
Positive mycelia color gradually turns yellow, and mycelia of gathering, and mycelia is insecure in conjunction with culture medium, and back side appearance is faint yellow.With
The extension of time, it is faint yellow slowly to deepen.Hyphae colorless has diaphragm, and in dendroid, branched hyphae alternate, bifurcation has excessive contracting
But it is unobvious, with major branch mycelia angle less than 90 DEG C, mycelia end blunt circle；Its spore is not yet observed at present.
Plate culture DK6 uses PDA culture medium, and the nutrient media components and formula are as follows: potato 200g, glucose
20g, agar 20g, supplement distilled water to 1000mL adjust pH to 7.0, in 121 DEG C of high pressure steam sterilization 30min；It is 25 DEG C, dark to train
It supports.
A kind of black fungus bacterial DK6 treats or prevents the application in nosomycosis biological agent drug in preparation, including the use of this
Bacterial strain is prepared into the drug of prevention and treatment plant botrytis as one of effective component, and the black fungus bacterial DK6 is especially suitable for anti-
Control graw mold of tomato and romaine lettuce gray mold.
Above-described application, protection content of the invention further include that the tunning of black fungus bacterial DK6 is in preparation grey mold
Application in bacterium spore germination inhibitor.
Protection content of the invention further includes application of the black fungus bacterial DK6 in preparation grey mold bacteria inhibitor.
A kind of black fungus bacterial DK6 is promoting the application in tomato growth, is used for including the black fungus bacterial DK6 fermentation liquid
Prepare tomato growth promotion preparation.
Compared with prior art, the invention has the following advantages that
1. the present invention filters out the new strains DK6 of one plant of prevention and treatment gray mold, which is basidiomycetes Auricularia, currently without
Relevant report about bacterial strain prevention and treatment gray mold.
2. the generation and sprawling of gray mold can be effectively inhibited with the fermentation liquid of the fungi.The DK6 bacterium that the present invention filters out
To botrytis cinerea in plate antagonistic effect, DK6 can cover continued growth on botrytis cinerea for strain, preliminary judgement its with botrytis cinerea be
Competition is the bacterial strain of one plant of preferable antagonistic activity；In excised leaf test, DK6 bacterial strain fermentation liquor prevents gray mold
Effect can effectively prevent the extension of scab up to 80%；Indoor pot experiment result table shows that the scab of DK6 processing group is equal
Smaller, disease index is smaller, and preventive effect is up to 57%.Romaine lettuce gray mold field efficacy (stable disease phase) the result shows that, DK6 fermentation
Liquid processing group is up to 73% to the preventive effect of gray mold, and preventive effect is higher.
3.DK6 fermentation liquid is in the germination test of tomato seeds, and DK6 fermentation liquid and DK6 tunning are to tomato seeds
Sprout no facilitation；For DK6 fermentation liquid in the test of tomato growth, DK6 bacterial strain has preferable growth-promoting functions to tomato,
It is variant significant (in 5% level) with control group in terms of stem thickness, plant height, root long, fresh weight, dry weight.
4. the bacterial strain that the present invention uses is higher to graw mold of tomato preventive effect, nontoxic to people and animals；It is at low cost；In addition also be suitble to pair
The prevention and treatment of romaine lettuce gray mold.
5. the present invention directly prevents and treats graw mold of tomato and romaine lettuce gray mold using the fermentation liquid of black fungus bacterial DK6 bacterial strain, raw
Equal very simple is produced and used, does not use organic solvent in production, reduces the pollution in production process and in product, also not
Solvent poisoning can be generated, has the advantages that biological pesticide to safety of human and livestock.
Detailed description of the invention
Fig. 1 is the colonial morphology of the DK6 bacterial strain of separation screening of the present invention.
Fig. 2 is the displaing micro picture of the DK6 bacterial strain of separation screening of the present invention, the picture under 100 times of mirrors.
Fig. 3 is the area the rDNA-ITS extension increasing sequence electrophoretogram of the DK6 bacterial strain of separation screening of the present invention.
It is the extension increasing sequence of DK6 bacterial strain in box.
Fig. 4 is antagonism and control of the DK6 bacterial strain to gray mold bacterial strain of separation screening of the present invention.
Wherein: 4A is control (not being inoculated with DK6 bacterial strain), and 4B shows DK6 in PDA culture medium to the short of money of gray mold bacterial strain
Anti- effect.
Fig. 5 is influence (3d) schematic diagram of the DK6 strain fermentation product of separation screening of the present invention to botrytis cinerea growth rate.
Wherein: 5A is control, and 5B is the suppression result that Fermentation Substance Concentration is 1%, and 5C is that Fermentation Substance Concentration is 5%
Suppression result, 5D are the suppression result that Fermentation Substance Concentration is 10%.
Fig. 6 is influence (7d) schematic diagram of the DK6 strain fermentation product of separation screening of the present invention to botrytis cinerea growth rate.
Wherein: 6A is control, and 6B is the suppression result that Fermentation Substance Concentration is 1%, and 6C is that Fermentation Substance Concentration is 5%
Suppression result, 6D are the suppression result that Fermentation Substance Concentration is 10%.
Fig. 7 is influence of the DK6 strain fermentation product of separation screening of the present invention to botrytis cinerea mycelia, the knot under 100 times of mirrors
Fruit figure.
Wherein: 7A is control (form of botrytis cinerea mycelia in the PDA culture medium without DK6 tunning)；7B is shown
The form of botrytis cinerea mycelia in PDA culture medium containing DK6 tunning；7C shows that the PDA containing DK6 tunning is cultivated
The form of botrytis cinerea mycelia on base.
Fig. 8 is influence schematic diagram of the DK6 strain fermentation product of separation screening of the present invention to botrytis cinerea spore.
Wherein: 8A is control (without the processing of DK6 tunning, sterile water process)；8B is shown through at DK6 tunning
The botrytis cinerea spore germination situation of reason.
Fig. 9 is that the DK6 strain fermentation product of separation screening of the present invention influences botrytis cinerea spore germination and germ tube length
Histogram.
Figure 10 is DK6 bacterial strain under the conditions of excised leaf to the inhibiting effect schematic diagram of graw mold of tomato.
Wherein: 10A is control (without DK6 fermentation liquor treatment, sterile water process), and 10B shows DK6 fermentation liquid to tomato
The inhibiting effect of gray mold.
Figure 11 is DK6 bacterial strain in pot experiment to the preventive effect schematic diagram of graw mold of tomato.
Wherein: 11A is control (without DK6 fermentation liquor treatment, sterile water process)；11B shows DK6 fermentation liquid to tomato
The inhibiting effect of gray mold.
Figure 12 is romaine lettuce gray mold field efficacy, the Growth of Lettuce situation schematic diagram of stable disease phase.
12A is control (without DK6 fermentation liquor treatment, sterile water process)；12B shows the life through DK6 fermentation liquor treatment
Dish growing state.
Figure 13 is influence of the DK6 bacterial strain to Tomato Seeds Germination.
Wherein: 13A is control (without DK6 fermentation liquor treatment, sterile water process)；13B shows DK6 fermentation liquid to tomato
The influence that seed is sprouted；13C shows influence of the DK6 tunning to Tomato Seeds Germination.
Figure 14 is influence schematic diagram of the DK6 bacterial strain fermentation liquor to tomato growth.
Wherein: 14A is control (without DK6 fermentation liquor treatment, sterile water process, from left to right three plants)；14B shows that DK6 is sent out
Influence of the zymotic fluid to tomato growth.
Figure 15 is influence schematic diagram of the DK6 bacterial strain fermentation liquor to tomato growth.
Wherein: 15A shows influence of the DK6 fermentation liquid to tomato growth；15B be control (without DK6 fermentation liquor treatment,
Sterile water process).
Specific embodiment
Technical solution of the present invention is if not otherwise specified the ordinary skill in the art.
Embodiment 1:
The separation and identification of DK6 bacterial strain
1, separately point, separation method (Morphological Identification of traditional microbiological)
Microorganism feature
The separation of DK6 bacterial strain: applicant separates from Chinese Shiyan City, Hubei Province maize leaf, screens and obtains one plant to ash
Mildew has the load bacteria strain of stronger control efficiency, and applicant is named as DK6.The separation of DK6 bacterial strain of the invention and
Screening uses the methods of tissue isolation, dilution plate method, plate antagonism, isolated test, and strain idenfication work is referring to " fungi
Identification handbook " (Wei Jingchao, 1979), the test method in the monographs such as " form and classification of fungi " (Dai Fanglan, 1987).It will
DK6 bacterial strain extracts genomic DNA in 25 DEG C of culture 10d, using CTAB method (Taylor et al, 1993), using fungi ribose
Body DNA universal primer ITS4 and ITS5 is expanded (Zhang Chulong and Xu are same, 2003) to its area rDNA-ITS.
DK6 strain morphology qualification result:
DK6 bacterial strain colony characteristics such as Fig. 1 are shown: after culture 7d, white is integrally presented in colony diameter about 7cm, bacterium colony.With
There is difference in the growth of incubation time, positive and negative color, and positive mycelia color gradually turns yellow, and gather mycelia, mycelia and culture medium
In conjunction with insecure, and the back side occur it is faint yellow.With the extension of time, faint yellow slowly deepen.
Microscope inspection characteristic pattern 2 shows that hyphae colorless has diaphragm, is in dendroid, branched hyphae alternate, bifurcation has excessive
It contracts but unobvious, with major branch mycelia angle less than 90 DEG C, mycelia end blunt circle；Its spore is not yet observed at present.
The primer sequence of ITS
Forward primer ITS4:5 '-TCCTCCGCTTATTGATATGC-3 '
Reverse primer ITS5:5 '-GGAAGTAAAAGTCGTAACAAGG-3 '
Sequence analysis shows, target fragment size be 675bp (Fig. 3).Extension increasing sequence BLAST analysis, it is known that itself and black wood
Ear (Auricularia auricula-judae) very high homology.
The bacterial strain is sent on January 18th, 2016 to China typical culture collection center preservation, classification naming:
Auricularia auricula-judae DK6；Deposit number: CCTCC NO:M 2016054；Address: Wuhan, China Wuhan
University.
Culture DK6 bacterial strain uses PDB culture solution, and component and formula are as follows: potato 200g, glucose 20g are mended
Distilled water is filled to 1000mL, pH to 7.0 is adjusted, in 121 DEG C of high pressure steam sterilization 30min.
Shake flask fermentation Parameter Conditions: with internal diameter be 9mm punch beat take DK6 fungus block be inoculated with, 25 DEG C, 160rpm, shake training 5d.
It shakes after training into its fermentation liquid.
Embodiment 2:
DK6 bacterial strain and botrytis cinerea plate dual test:
Activated botrytis cinerea is beaten with the punch that internal diameter is 9mm to connect in the side of PDA plate, it is another to flank DK6 bacterium
Strain (internal diameter 9mm), is spaced about 5cm between DK6 bacterial strain and botrytis cinerea, do not connect work in the PDA plate side inoculation botrytis cinerea other side
For control, it is repeated 3 times.Observation face-off result after 3~4d.Test result is shown in Fig. 4.
Dual test is as the result is shown: DK6 bacterial strain can cover continued growth above botrytis cinerea, and grow on botrytis cinerea
It is more dense, according to a preliminary estimate DK6 bacterial strain can with botrytis cinerea compete nutrition or can using botrytis cinerea idiotrophic or its
Metabolite continued growth, the mode of action are nutrient competition, for one plant of bacterial strain with the effect of botrytis cinerea high inhibition.
Embodiment 3:
The preliminary analysis of bacterial strain DK6 antagonism
(1) influence of the DK6 tunning to botrytis cinerea growth rate
It is that 9mm punch is beaten and takes DK6 fungus block with internal diameter, is transferred in 100mL PDB, 25 DEG C, 160rpm shakes bacterium 5d.It will system
The fermentation liquid got ready is centrifuged 10min through 8,000r/min, and supernatant is filtered with 0.22 μm of miillpore filter, obtains without fermented liquid
That is fermented supernatant fluid.Inverted plate in PDA is added by 1% (volume ratio), 5%, 10% in fermented supernatant fluid, is connect on each plate straight
Diameter is the botrytis cinerea mycelia block of 9mm, 3 repetitions of each processing, 3 parallel tests, 20 DEG C of cultures, respectively at 3d, 5d, 7d measurement
The colony diameter of botrytis cinerea simultaneously records related data.Test result is Fig. 5, Fig. 6.
Fig. 5 is the results show that when 3d, and the tunning of DK6 bacterial strain is more apparent to the inhibiting effect of botrytis cinerea, processing group mycelia
It is sparse compared with control group.Compared with the control, the processing group of 1%, 5% concentration, botrytis cinerea mycelia center and edge are relatively close and not
Rule, the processing group of 10% concentration, botrytis cinerea mycelia center is closeer, and edge is more sparse.
Fig. 6 is the results show that when 7d, and the tunning of DK6 bacterial strain is unobvious to the inhibiting effect of botrytis cinerea, but with concentration
Increase, inhibiting rate relative increase.
Influence (20 DEG C) of the 1 DK6 tunning of table to botrytis cinerea growth rate
Note: letter is different in table indicates significant difference (17.0 LSD method of SPSS, P≤0.05)
Influence of the DK6 tunning to botrytis cinerea growth rate shows: the tunning of DK6 bacterial strain cannot effectively inhibit
Botrytis cinerea growth, but with the increase of concentration, inhibiting rate increased, and only up to reach 18.6%.In the same period,
10% is preferable with the inhibitory effect of 5% concentration, and the inhibiting rate difference of the two is not significant, but 10% concentration processing group with 1% place
Reason group difference is more significant.In same concentration level, when 3d, tunning reaches highest (table 1) to the inhibiting rate of botrytis cinerea.
(2) influence of the DK6 tunning to botrytis cinerea mycelia
Specific test method is the same as (1).When 3d, the metamorphosis of microscopically observation botrytis cinerea mycelia.Test result is figure
7.Fig. 7 is the results show that DK6 tunning has a certain impact to the hypha form of botrytis cinerea, with normal botrytis cinerea mycelia
It compares, botrytis cinerea mycelia is thicker, and branch is shorter and more, and it is inside mycelia that vacuole is presented more, and particle is relatively more.
(3) influence of the DK6 tunning to botrytis cinerea spore germination
DK6 fungus block is taken through beating for 9mm punch with interior, is transferred in 100mL PDB, 25 DEG C, 160rpm shakes bacterium 5d.It will system
The spore suspension got ready is centrifuged 10min through 8,000r/min, and supernatant is filtered with 0.22 μm of miillpore filter, obtains sterile hair
Zymotic fluid, that is, fermented supernatant fluid.The preparation of botrytis cinerea spore liquid is same as above.According to following proportion system: fermentation liquid: spore liquid: water: PDB
=100:100:100:50 (volume ratio) is added in concave-concave glass slide, 20 DEG C of cultures, spore germination situation is observed after 4h, every 4h
Observation 1 time.
Test result is shown in Fig. 8,9.Fig. 8 is the results show that DK6 tunning being affected to the spore of botrytis cinerea, normally
Botrytis cinerea spore sprouted and germ tube is very long, and the spore of DK6 tunning processing is almost without sprouting, even if sprouting,
Germ tube relative comparison group is short.Fig. 9 is the results show that influence of the DK6 tunning to the spore of botrytis cinerea is very big, when 8h, normally
Botrytis cinerea spore germination rate is up to 92.9%, and germ tube length has 34.8 μm, and the spore germination rate of DK6 tunning processing only has
3.7%, the spore germ tube of sprouting only has 9.2 μm (tables 2).In 5% level, two aspect of 8h germination rate and germ tube length, control
There were significant differences with DK6 processing group for group.
2 DK6 tunning of table influences (3d, 20 DEG C) to botrytis cinerea spore
Note: letter is different in table indicates significant difference (17.0 t of SPSS is examined, P≤0.05)
The above result shows that: the influence that DK6 tunning extends the spore germination of botrytis cinerea and germ tube is very big, when 8h,
In terms of germination rate, normal botrytis cinerea spore germination rate is up to 92.9%, and the spore germination rate of DK6 tunning processing is only
Have 3.7%,；In terms of germ tube length, normal botrytis cinerea spore germ tube length has 34.8 μm, the spore sprouted after DK6 processing
Germ tube only has 9.2 μm.In 5% level, 8h germination rate and the aspect of germ tube length two, control group and DK6 processing group difference compared with
Significantly.
Embodiment 4:
Excised leaf measurement:
It is that 9mm punch is beaten in the PDB for taking DK6 fungus block to be inoculated in bacterium of having gone out (every bottle of 100mL) with internal diameter, 25 DEG C,
It is spare after 160rpm culture 5d.The fresh tomato blade that field acquires is rinsed well in the tap water of flowing, naturally dry
After be dipped in fermentation liquid (after 8 layers of filtered through gauze) and impregnate 30min, impregnated using sterile water as compareing, each processing sets 10
Blade, 3 repetitions.After processing, botrytis cinerea Tip Splitting block after 2~3d of same position inoculated and cultured of each blade, bacterium
Block diameter is 6mm.Certain humidity is kept in porcelain dish, gummed paper film is covered and is placed in 20 DEG C of constant temperature illumination cultivation rooms, is seen daily
Incidence is examined, and measures its Lesion size.Test result is as shown in Figure 10, and inhibiting effect equally shows under the conditions of excised leaf
Stronger inhibiting effect out.
The in vitro lower DK6 bacterial strain of table 3 is to graw mold of tomato inhibiting rate (3d, 20 DEG C)
Note: letter is different in table indicates significant difference (17.0 t of SPSS is examined, P≤0.05)
In excised leaf test, control group just starts to fall ill after 2d in inoculation botrytis cinerea mycelia block, and morbidity is serious when 3d,
Lesion size is that 5.5~4cm is differed, and incidence of leaf has also rotted, and the phenomenon that wilting is presented in part of not falling ill.DK6 fermentation
Liquid processing group scab mean size is 0.77cm, and inhibiting rate reaches 79.6% (table 3), further demonstrates DK6 and has to gray mold
Have and is acted on compared with high inhibition.The tomato leaf scab that DK6 fermentation liquid impregnates is smaller, is able to suppress graw mold of tomato.Lesion size side
Face, in 5% level, with control group Lesion size significant difference；In terms of inhibiting rate, inhibiting rate of the DK6 fermentation liquid to gray mold
Up to 80%, inhibiting effect is significant.
Embodiment 5:
Graw mold of tomato preventive effect pot experiment
By internal diameter be 9mm DK6 strain inoculated in PDB (every bottle of 100mL), after 25 DEG C, 160rpm, shaking table culture 5d at
Its fermentation liquid, 8 layers of gauze filter out spare after mycelium pellet.The conidium liquid of botrytis cinerea, hemocytometer are obtained with the method for washing spore
Number plate adjusts its concentration after counting be 105A/mL is spare.Get out the consistent tomato seedling of growing way (about 20d), sprinkling DK6 hair
20mL is sprayed in zymotic fluid, each processing, sprays the botrytis cinerea spore liquid of equivalent afterwards for 24 hours, repeats test 3 times.After test process 2d respectively
The incidence for observing tomato seedling is classified by grade scale.Test result such as Figure 11 and table 4.
Blade disease scale method (as unit of blade)
0 grade: disease-free spot
1 grade: scab leaf area < leaf area 1/4；
2 grades:>1/4,<1/2；
3 grades:>1/2,<3/4；
4 grades: > 3/4.
4 graw mold of tomato preventive effect results from pot experiment test of table (3d, 20 DEG C)
Note: letter is different in table indicates significant difference (17.0 t of SPSS is examined, P≤0.05)
In pot experiment, inoculation botrytis cinerea spore liquid for 24 hours after, occur a small amount of scab on control group tomato leaf and scab compared with
Small, then scab slowly extends.And just there is a small amount of scab to occur after the romaine lettuce blade 2d of DK6 processing group, then scab is just slowly
Extension.After 3d, there is more scab in control group, and has sprayed the tomato leaf of DK6 fermentation liquor treatment in advance, and scab is less,
Lesion area is relatively small, illustrates after having sprayed DK6 fermentation liquid in advance, is able to suppress growth and expansion of the gray mold on tomato leaf
Greatly, i.e. DK6 can effectively prevent the generation of graw mold of tomato.
Embodiment 6:
Romaine lettuce gray mold crop field efficiency test
By internal diameter be 9mm DK6 strain inoculated in PDB (every bottle of 100mL), after 25 DEG C, 160rpm, shaking table culture 5d at
Its fermentation liquid is spare.The conidium liquid of botrytis cinerea is obtained with the method for washing spore, 8000rpm is centrifuged 5min, takes supernatant, blood
Ball count plate counts and conidium liquid is adjusted to 106A/mL is spare.Processing group be DK6 fermentation liquor treatment group (3 cells, often
Square sprinkling 100mL fermentation liquid), in addition be arranged 3 blank controls.
It waters in advance, with hand sprayer by DK6 fermentation liquid even spraying in each position of romaine lettuce plant, blank control group is only
Spray the clear water of equivalent.After for 24 hours, with hand sprayer sprinkling and the isometric botrytis cinerea spore liquid of DK6 fermentation liquid, even spraying
In each position of romaine lettuce plant, vegetable plot is covered with plastic cloth, guarantees humidity.The disease index of romaine lettuce is investigated in the stable disease phase,
And make relevant data analysis.
Romaine lettuce gray mold disease leaf Seriousness gradation standard:
0 grade: blade disease-free spot；
1 grade: scab accounts for 5% or less full leaf area；
3 grades: scab accounts for the 5% of full leaf area up to 10% or less；
5 grades: scab accounts for the 11% of full leaf area up to 25% or less；
7 grades: scab accounts for the 26% of full leaf area up to 50% or less；
9 grades: 50% or more or the blade that scab accounts for full leaf area are withered.
Disease index=(the disease numbers of sheets at different levels × series/total number of sheets of falling ill accordingly × highest morbidity series) × 100
Control efficiency (%)=(control group disease index-processing group disease index/control group that is averaged that is averaged is averaged the state of an illness
Index) × 100%
Stable disease phase test result is shown in Figure 12.
5 romaine lettuce gray mold crop field preventive effect (stable disease phase) of table
Note: letter is different in table indicates significant difference (17.0 t of SPSS is examined, P≤0.05)
Romaine lettuce gray mold field efficacy (stable disease phase) the results are shown in Table 5, the romaine lettuce diseased plant through DK6 fermentation liquor treatment cell
Rate only has 72.5%, differs greatly with control group；Disease index is significantly lower than control group.DK6 fermentation liquor treatment group Growth of Lettuce
Neat healthy and strong, sick leaf is few, and growing way is vigorous, and disease is relatively light, and DK6 fermentation liquid is to the preventive effect of romaine lettuce gray mold up to 72.9%.
The result shows that DK6 has good biological and ecological methods to prevent plant disease, pests, and erosion value, the later period can carry out the research of Antagonizing to it, develop relevant microbial inoculum simultaneously
It promotes and applies.
Embodiment 7:
The influence of DK6 fermentation liquid and tunning to Tomato Seeds Germination
By DK6 strain inoculated in PDB (every bottle of 100mL), the fungus block that internal diameter is 9mm is connect.It shakes after training 5d into its fermentation liquid.
DK6 fermentation liquid is obtained after 8 layers of filtered through gauze, 0.2 μm of biofilter filtering fermentating liquid obtains tunning.Fermentation liquid, fermentation
After product obtains, it is spare 7.0 to adjust its pH.Tomato seeds of the same size (middle vegetable No. four) is selected, is first impregnated with sterile water
After 20h, it is respectively put into fermentation liquid (DK6), impregnates 20min again in tunning (DK6-f), is put into the culture for being covered with wet filter paper
In ware, 28 DEG C of dark cultures, timing adding water keeps moistening 50 seeds of each processing, be repeated 3 times.The sprouting of seed is observed after 3d
Situation, and record related data.Test result such as Figure 13, the tomato species handled as the result is shown through DK6 fermentation liquid and tunning
Son, after 5d, the tomato root long and control group of sprouting do not have difference.Also without difference in terms of tomato plant height.
Influence (5d, 28 DEG C) of 6 different disposal of table to Tomato Seeds Germination
Note: letter is different in table indicates significant difference (17.0 LSD method of SPSS, P≤0.05)
The tomato seeds handled through DK6 fermentation liquid and tunning DK6-f, after 5d, the tomato root long and control group of sprouting
In 5% horizontal no significant difference.In terms of fresh weight (30 tomato young plant gross weights), DK6-f processing group is most heavy, DK6 group time
It, control group is minimum, and in 5% level, significant difference between three.In terms of germination rate, DK6-f group germination rate reaches
56%, DK6 group have 51.5% or so (table 6), and for the two in 5% level, difference is not significant, and with control group significant difference.
Embodiment 8:
Influence of the DK6 bacterial strain fermentation liquor to tomato growth
The acquisition of DK6 fermentation liquid is the same as embodiment 7.Tomato seeds of the same size (middle vegetable No. four) is selected, with DK6 fermentation liquid
20min is impregnated, is sowed in the hole tray equipped with wet Nutrition Soil, 15 seeds of each processing are repeated 3 times.It places it in
25 DEG C, cultivate in the greenhouse of 12h illumination.After sowing 7d, 14d, 2mL fermentation liquid is injected in every plant of tomato hole.It is seen after 40d
Tomato growth situation is examined, related data is measured.
Test result such as Figure 14,15, the tomato seedling of 40d size as the result is shown, for control group, DK6 fermentation liquid
One group of tomato growing way of processing is more preferable, and plant height is much larger than control group, two groups of significant differences in terms of plant height
Growth-promoting functions (40d, 25 DEG C) of 7 DK6 of table to tomato seedling
Note: letter is different in table indicates significant difference (17.0 t of SPSS is examined, P≤0.05)
By the tomato seedling of 40d size, the result such as table 7 measured after clean dry is extracted after taking pictures in soil, is as a result shown
Show that DK6 bacterial strain fermentation liquor there are preferable growth-promoting functions to tomato.In terms of stem thickness, DK6 processing group tomato stem thickness has 3.52mm, compares
Group tomato stem thickness has 3.07mm, and the two is in 5% horizontal upper significant difference.In terms of plant height, DK6 processing group tomato plant height has
18.12cm, control group tomato plant height have 12.86cm, and the two is in 5% horizontal upper significant difference.In terms of root long, DK6 processing group kind
Eggplant root long has 6.95cm, and control group tomato root is with 4.97cm, and the two is in 5% horizontal upper significant difference.(15 kinds in terms of fresh weight
Eggplant young plant gross weight), DK6 processing group tomato fresh weight has 54.83g, and control group tomato fresh weight has 34.47g, and the two is in 5% level
Significant difference.In terms of dry weight (15 tomato young plant gross weights), DK6 processing group tomato dry weight has 3.18g, and control group tomato dry weight has
2.17g, the two is in 5% horizontal upper significant difference.Test is repeated 3 times, and 3 test results can illustrate 6 bacterium of DK than more consistent
Strain has good growth-promoting functions to the growth of tomato.
Claims (6)
1. a kind of isolated black fungus bacterial, it is characterised in that: the bacterial strain is black fungus bacterial (Auricularia
auricula-judae)DK6；Deposit number: CCTCC NO:M 2016054.
2. black fungus bacterial described in claim 1 treats or prevents the application in plant botrytis preparation in preparation.
3. the tunning of black fungus bacterial described in claim 1 is preparing the application in botrytis cinerea spore germination inhibitor.
4. application of the black fungus bacterial described in claim 1 in preparation grey mold bacteria inhibitor.
5. application of the black fungus bacterial described in claim 1 in preparation tomato growth promotor.
6. application according to claim 2, the plant is romaine lettuce or tomato.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514331A (en) * | 2009-01-05 | 2009-08-26 | 中国农业大学 | Pseudomonas. chlororaphis subsp. Aurantiaca Pa40 and application thereof |
| CN101643705A (en) * | 2009-09-11 | 2010-02-10 | 东北农业大学 | Gliocladium spp. strain for suppressing botrytis cinerea pers |
| CN101961014A (en) * | 2010-03-19 | 2011-02-02 | 中国计量学院 | Method for preparing microbial pesticide for preventing and controlling fungal diseases of vegetables |
| CN104962501A (en) * | 2015-07-20 | 2015-10-07 | 云南大学 | Preparation and application of bacterial strain resistant to gray mould of vegetables and fruits and antagonist |
| CN105062897A (en) * | 2015-08-14 | 2015-11-18 | 潍坊万胜生物农药有限公司 | Thichoderma viride with high chlamydospore yield and application of thichoderma viride |
| CN105176894A (en) * | 2015-11-03 | 2015-12-23 | 河北省农林科学院植物保护研究所 | Bacillus amyloliquefaciens for controlling gray mold of tomato and microbial inoculant thereof |
-
2016
- 2016-01-26 CN CN201610050570.0A patent/CN105624046B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514331A (en) * | 2009-01-05 | 2009-08-26 | 中国农业大学 | Pseudomonas. chlororaphis subsp. Aurantiaca Pa40 and application thereof |
| CN101643705A (en) * | 2009-09-11 | 2010-02-10 | 东北农业大学 | Gliocladium spp. strain for suppressing botrytis cinerea pers |
| CN101961014A (en) * | 2010-03-19 | 2011-02-02 | 中国计量学院 | Method for preparing microbial pesticide for preventing and controlling fungal diseases of vegetables |
| CN104962501A (en) * | 2015-07-20 | 2015-10-07 | 云南大学 | Preparation and application of bacterial strain resistant to gray mould of vegetables and fruits and antagonist |
| CN105062897A (en) * | 2015-08-14 | 2015-11-18 | 潍坊万胜生物农药有限公司 | Thichoderma viride with high chlamydospore yield and application of thichoderma viride |
| CN105176894A (en) * | 2015-11-03 | 2015-12-23 | 河北省农林科学院植物保护研究所 | Bacillus amyloliquefaciens for controlling gray mold of tomato and microbial inoculant thereof |
Non-Patent Citations (3)
| Title |
|---|
| "一种新的杀线虫担子菌";李国红等;《云南大学学报（自然科学版）》;20011231;第23卷(第2期);第149-152页 * |
| "木耳优良杂交菌株的初步筛选";桑峰;《中国优秀硕士学位论文全文数据库 农业科技辑》;20120515;第1-56页 * |
| "木耳杂交菌株对绿色木霉菌的抗性测定";桑峰等;《菌物研究》;20110930;第9卷(第3期);168-171,175页 * |
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