CN105616618A - Application of murraya exotica in preparing cholagogue medicine - Google Patents
Application of murraya exotica in preparing cholagogue medicine Download PDFInfo
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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Abstract
The invention discloses application of murraya exotica in preparing a cholagogue medicine, in particular novel application of murraya exotica in preparing the cholagogue medicine.
Description
Technical field
The present invention relates to the novelty teabag of Leaf and twig of Common Jasminorange, namely prepare the purposes in Cholagogue drug.
Background technology
The biliary tract such as cholelithiasis, cholecystitis is worldwide common disease and frequently-occurring disease. For cholelithiasis, cholelithiasis occurs in gall-bladder, stones in intrahepatic bile duct and common bile duct. Its morbidity is different because of the factor such as race, heredity, geography, diet. The eighties in 20th century, the developed country such as the U.S., Germany cholelithiasis incidence is about 10%��15%. Total recall rate of China's cholelithiasis is 6.6%, and the recall rate of different areas is between 3%��11%, and crowd's sickness rate of 80 years old is up to 23%. Cholelithiasis has dividing of cholesterol calculus, bile pigment calculus and mixing calculus, and great majority are cholesterol calculus, account for about 90%. In recent years, owing to standard of living improves constantly, in diet, the intake such as fat, protein increases, and cholelithiasis sickness rate obviously rises, and patient gets more and more. Meanwhile, the sickness rate of cholecystitis also obviously increases, and the cholecystitis of the overwhelming majority all has cause-effect relationship with gallbladdergallstonecholetithiasis.
Often there is epigastrium angina, the shiver with cold symptom such as heating, jaundice in gallbladdergallstonecholetithiasis, can cause that bile duct blocks, canceration, liver be impaired and the harm such as acute pancreatitis, seriously affects patient's physical and mental health.
Cholelithiasis can be had an operation treatment, but most of people is still based on pharmacological agent, only just selects operative treatment under critical condition. Therefore, the clinical demand to choleretic grows with each passing day.
Choleretic, refers to and can promote choleresis, strengthens gall-bladder and shrinks, promotes the medicine of gall bladder emptying. Clinical it is mainly used in the biliary tract such as cholecystitis, cholelithiasis. According to its effect classification, it is divided into and promotes secreting courage medicine and promoting row's courage medicine of bile excretion of choleresis.
Take a broad view of Western medicine choleretic market, not only Cholagogue drug kind is few for many years, and great majority are that China started to produce in 70��eighties of 20th century, (such as the kind such as dehydrocholic acid, oxaphenamide, Nicotinylmythylamide Hydroxymethylnicotinamide, phenylpropyl alcohol, ursodesoxycholic acid, hydroxyl first fragrant beautiful element, Armillarisin A), nearly one, Two decades years seldom has new drug to release, and its curative effect is also as one wishes not to the utmost. In present China market, having some Chinese patent medicine choleretics, such as cholagogic tablet, relax bladder, XIAOYAN LIDAN PIAN, cholagogic and lithagogue tablet, compound dantong tablets, DANSHU JIAONANG, cholelith open capsule etc., can meet patient demand to a certain extent, but curative effect is still not very good. Generally speaking, choleretic present situation can not meet demand clinical at present. Therefore the new choleretic that research and development good effect, side effect are little it is badly in need of.
Leaf and twig of Common Jasminorange is dry leave and the band leaf spray of rutaceae Murraya paniculata (L.) Jack Murrayapaniculata (L.) Jack. Taste hardship pungent, micro-, warm in nature, there is effect of promoting the circulation of QI to relieve pain, promoting blood circulation to remove blood stasis. Existing documents and materials display Leaf and twig of Common Jasminorange have analgesia, inflammation antibacterial, anti-, anesthesia, anti-oxidant, suppress gastrointestinal movement and alleviate the pharmacological actions such as diabetic nephropathy renal impairment. But have no the document about choleretic effect to report.
Summary of the invention
It is an object of the invention to provide the purposes of Leaf and twig of Common Jasminorange in preparation Cholagogue drug;
It is a further object of the present invention to provide the purposes of Folium Et Cacumen Murrayae extract in preparation Cholagogue drug;
It is a further object of the present invention to provide the purposes of Leaf and twig of Common Jasminorange ethyl acetate extract in preparation Cholagogue drug;
Leaf and twig of Common Jasminorange is dry leave and the band leaf spray of rutaceae Murraya paniculata (L.) Jack Murrayapaniculata (L.) Jack. Taste hardship pungent, micro-, warm in nature, there is effect of promoting the circulation of QI to relieve pain, promoting blood circulation to remove blood stasis, cure mainly stomachache, rheumatic arthralgia, toothache, tumbling and swelling, worm snakebite etc. are treated in external application smelting.
Contriver adopts and wins the direct weighting method observation Leaf and twig of Common Jasminorange alcohol extract of mouse gall-bladder, ethyl acetate extract and aqueous extract to the impact of normal mouse and ligation bile duct mouse choleresis and excretion; Rat biliary drainage method is adopted to observe Leaf and twig of Common Jasminorange alcohol extract, ethyl acetate extract to the impact of normal rat bile flow and bile component thereof. Find that Leaf and twig of Common Jasminorange alcohol extract, ethyl acetate extract can significantly alleviate the bile coefficient of normal mouse, significantly increase the bile coefficient of ligation bile duct mouse; Can significantly increase the bile output of biliary drainage rat, significantly reduce rat bile total cholesterol level simultaneously. Show that Leaf and twig of Common Jasminorange alcohol extract and ethyl acetate extract can promote secretion and the excretion of bile, there is choleretic effect. And Leaf and twig of Common Jasminorange aqueous extract has the bile coefficient alleviating normal mouse and increases the trend of the bile coefficient of ligation bile duct mouse, namely has the trend of choleretic effect.
Therefore, applicant provides the purposes of Leaf and twig of Common Jasminorange in preparation Cholagogue drug; The particularly purposes of extract after extraction into ethyl acetate of Folium Et Cacumen Murrayae extract, Leaf and twig of Common Jasminorange ethanol extraction and Leaf and twig of Common Jasminorange ethanol extraction in preparation Cholagogue drug.
It is a further object of the present invention to provide the preparation method of Leaf and twig of Common Jasminorange acetic acid ethyl ester extract.
The preparation method of described Leaf and twig of Common Jasminorange acetic acid ethyl ester extract
Comprise the following steps:
By Leaf and twig of Common Jasminorange medicinal material drying, pulverize, first with extraction using alcohol, reclaim ethanol and concentrate, make alcohol extract; Being cleaned by alcohol extract petroleum ether extraction, remove the composition being dissolved in sherwood oil, the alcohol extract after then being cleaned by petroleum ether extraction by ethyl acetate extracts, and carries out acetic acid ethyl ester extract reclaiming ethyl acetate, and concentrates and be prepared from.
Concrete, the extracting method of Leaf and twig of Common Jasminorange acetic acid ethyl ester extract of the present invention comprises the following steps:
A. Leaf and twig of Common Jasminorange medicinal material drying, the alcohol heating reflux being more than 50% by concentration expressed in percentage by volume after pulverizing extracts, and filters, and filtrate recycling ethanol is to without alcohol taste, concentrating to obtain ethanol extraction A;
B. being added water by ethanol extraction A and make suspension, with petroleum ether extraction, abandon sherwood oil liquid, remainder is divided into extract B;
C. extract B being extracted with ethyl acetate, extraction liquid reclaims ethyl acetate and is condensed into cream, obtains Leaf and twig of Common Jasminorange ethyl acetate extract.
Preferably,
The extracting method of described Leaf and twig of Common Jasminorange acetic acid ethyl ester extract comprises the following steps:
A. getting Leaf and twig of Common Jasminorange medicinal material drying, after pulverizing, after being equivalent to the alcohol immersion 0.5-2h of Leaf and twig of Common Jasminorange weight 4-12 concentration expressed in percentage by volume 50%-95% doubly, heating and refluxing extraction 1-3h, filters, and collects filtrate; The alcohol heating reflux that the dregs of a decoction add the concentration expressed in percentage by volume 50%-95% being equivalent to Leaf and twig of Common Jasminorange weight 4-8 times extracts twice, each 1-3h, filters, and collects filtrate; Merge 3 filtrates, reclaim ethanol to without alcohol taste, concentrating to obtain ethanol extraction A;
B. below ethanol extraction A greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ethanol extraction A, in ice cube thaw process, constantly grinding makes ethanol extraction A dissolve and makes suspension; Control suspension temperature, below 10 DEG C, then carries out extraction with sherwood oil and cleans, remove the composition being dissolved in sherwood oil, abandon sherwood oil extraction liquid, and remainder is divided into extract B;
C. extract B ethyl acetate solution is fully extracted, collect extraction liquid, reclaim ethyl acetate and be condensed into medicinal extract, obtain described Leaf and twig of Common Jasminorange ethyl acetate extract.
The present invention also provides Folium Et Cacumen Murrayae extract and pharmaceutically acceptable carrier combination to be formed in the novelty teabag of preparation Cholagogue drug aspect. Folium Et Cacumen Murrayae extract coordinates pharmaceutically acceptable carrier, is prepared into tablet, granule, capsule by this area ordinary method, drips the formulation such as ball, tincture.
The pharmaceutically acceptable carrier of the present invention includes but not limited to following:
Thinner: starch, lactose, dextrin, Microcrystalline Cellulose, inorganic salt etc.
Wetting agent and tackiness agent: purified water, ethanol, gelatin, polyoxyethylene glycol, derivatived cellulose etc.
Disintegrating agent: starch, sodium starch glycolate, derivatived cellulose, polyvinylpolypyrrolidone etc.
Lubricant: Magnesium Stearate, micropowder silica gel, talcum powder, polyoxyethylene glycol etc.
Solubility promoter: water, ethanol, glycerine, propylene glycol, Liquid Paraffin, vegetables oil etc.
Correctives: sucrose, monose, perfume compound etc.
Sanitas: phenylformic acid, Sorbic Acid, methyl esters, second ester, propyl ester etc.
Existing documents and materials display Leaf and twig of Common Jasminorange have analgesia, inflammation antibacterial, anti-, anesthesia, anti-oxidant, suppress gastrointestinal movement and alleviate the pharmacological actions such as diabetic nephropathy renal impairment. But have no the document about choleretic effect to report.
The novelty teabag that Leaf and twig of Common Jasminorange provided by the invention, Folium Et Cacumen Murrayae extract prepare Cholagogue drug aspect has the following advantages:
1, Folium Et Cacumen Murrayae extract choleretic effect is satisfactory for result. On the impact of normal mouse and ligation bile duct mouse choleresis and excretion, and on test-results displays such as the impacts of normal rat bile flow and bile component thereof: Folium Et Cacumen Murrayae extract of the present invention can significantly alleviate the bile coefficient of normal mouse, significantly increases the bile coefficient of ligation bile duct mouse; Can significantly increase the bile output of biliary drainage rat, significantly reduce rat bile total cholesterol level simultaneously. Prompting the present invention can promote secretion and the excretion of bile, has choleretic effect, provides new basic substance for exploitation becomes Cholagogue drug, has potential huge Social benefit and economic benefit.
2, Leaf and twig of Common Jasminorange herb resource enriches, and extracts a large amount of preparations that preparation method is simple, raw material sources are conveniently easy to get, can be used for Folium Et Cacumen Murrayae extract.
Embodiment
Embodiment 1
A. Leaf and twig of Common Jasminorange medicinal material 1kg is got, dry, after pulverizing, put in extraction vessel, add 4L95% alcohol immersion 0.5h, heating and refluxing extraction 1.5h, filter, collect filtrate. The dregs of a decoction add 95% ethanol 5L, extracts 1.0h; Filter, collect filtrate; The dregs of a decoction add 95% again, and ethanol 4L extracts 1.0h; Filter, collect filtrate. Merge 3 filtrates, reclaim ethanol to without alcohol taste, concentrating to obtain ethanol extraction A;
B. below ethanol extraction A greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ethanol extraction A, in ice cube thaw process, constantly grinding makes ethanol extraction A dissolve and makes suspension; Control suspension temperature, below 10 DEG C, then carries out extraction with sherwood oil and cleans, remove the composition being dissolved in sherwood oil, abandon sherwood oil extraction liquid, and remainder is divided into extract B;
C. extract B ethyl acetate solution is fully extracted, collect extraction liquid, reclaim ethyl acetate and be condensed into medicinal extract, obtain described Leaf and twig of Common Jasminorange ethyl acetate extract.
Embodiment 2
A. Leaf and twig of Common Jasminorange medicinal material 1kg is got, dry, after pulverizing, put in extraction vessel, add 8L75% alcohol immersion 1.0h, heating and refluxing extraction 1h, filter, collect filtrate. The dregs of a decoction add 75% ethanol 6L, extracts 2h; Filter, collect filtrate; The dregs of a decoction add 95% again, and ethanol 6L extracts 1h; Filter, collect filtrate. Merge 3 filtrates, reclaim ethanol and be condensed into cream, obtain Leaf and twig of Common Jasminorange ethanol extraction A.
B. below ethanol extraction A greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ethanol extraction A, in ice cube thaw process, constantly grinding makes ethanol extraction A dissolve and makes suspension; Control suspension temperature, below 10 DEG C, then carries out extraction with sherwood oil and cleans, remove the composition being dissolved in sherwood oil, abandon sherwood oil extraction liquid, and remainder is divided into extract B;
C. extract B ethyl acetate solution is fully extracted, collect extraction liquid, reclaim ethyl acetate and be condensed into medicinal extract, obtain described Leaf and twig of Common Jasminorange ethyl acetate extract.
Embodiment 3
A. get Leaf and twig of Common Jasminorange medicinal material 1kg, pulverize and sieve, put in extraction vessel, add 10L60% alcohol immersion 1.5h, heating and refluxing extraction 2.0h, filter, collect filtrate. The dregs of a decoction add 60% ethanol 4L, extracts 3h; Filter, collect filtrate; The dregs of a decoction add 60% again ethanol 8L refluxing extraction 1h; Filter, collect filtrate. Merge 3 filtrates, reclaim ethanol and be condensed into cream, obtain Leaf and twig of Common Jasminorange ethanol extraction A.
B. below ethanol extraction A greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ethanol extraction A, in ice cube thaw process, constantly grinding makes ethanol extraction A dissolve and makes suspension; Control suspension temperature, below 10 DEG C, then carries out extraction with sherwood oil and cleans, remove the composition being dissolved in sherwood oil, abandon sherwood oil extraction liquid, and remainder is divided into extract B;
C. extract B ethyl acetate solution is fully extracted, collect extraction liquid, reclaim ethyl acetate and be condensed into medicinal extract, obtain described Leaf and twig of Common Jasminorange ethyl acetate extract.
Embodiment 4
A. get Leaf and twig of Common Jasminorange medicinal material 1kg, pulverize and sieve, put in extraction vessel, add 12L50% alcohol immersion 2h, heating and refluxing extraction 3.0h, filter, collect filtrate. The dregs of a decoction add 50% ethanol 8L, extracts 2.0h; Filter, collect filtrate; The dregs of a decoction add 50% again ethanol 8L refluxing extraction 2.0h; Filter, collect filtrate. Merge 3 filtrates, reclaim ethanol and be condensed into cream, obtain Leaf and twig of Common Jasminorange ethanol extraction A.
B. below ethanol extraction A greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ethanol extraction A, in ice cube thaw process, constantly grinding makes ethanol extraction A dissolve and makes suspension; Control suspension temperature, below 10 DEG C, then carries out extraction with sherwood oil and cleans, remove the composition being dissolved in sherwood oil, abandon sherwood oil extraction liquid, and remainder is divided into extract B;
C. extract B ethyl acetate solution is fully extracted, collect extraction liquid, reclaim ethyl acetate and be condensed into medicinal extract, obtain described Leaf and twig of Common Jasminorange ethyl acetate extract.
Embodiment 5
Getting Leaf and twig of Common Jasminorange medicinal material 0.45kg, pulverize and sieve, put in extraction vessel, the 10L that adds water soaks 1h, heated and boiled, keeps micro-1h that boils, and filters, and collects filtrate. The dregs of a decoction add water 10L, extract 2 times, each 1h, filter, and collect filtrate. Merge 3 filtrates, it is settled to 1000ml with distilled water after concentrated, obtains Leaf and twig of Common Jasminorange water extract. 4 DEG C of Refrigerator stores, face the used time and mix even.
Embodiment 6
Extract 5ml in Example 5, is settled in the volumetric flask of 10ml with distilled water, to obtain final product. 4 DEG C of Refrigerator stores, face the used time and mix even.
Embodiment 7
Getting Leaf and twig of Common Jasminorange medicinal material 0.45kg, pulverize and sieve, put in extraction vessel, after being equivalent to the alcohol immersion 1h of concentration expressed in percentage by volume 95% of Leaf and twig of Common Jasminorange weight 8 times, heating and refluxing extraction 2h, filters, and collects filtrate; The alcohol heating reflux that the dregs of a decoction add the concentration expressed in percentage by volume 95% being equivalent to Leaf and twig of Common Jasminorange weight 6 times extracts twice, each 1.5h, filters, and collects filtrate; Merge 3 filtrates, reclaim ethanol to without alcohol taste, concentrating to obtain ointment. Below ointment greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ointment, in ice cube thaw process, constantly grinding makes ointment dissolve and makes suspension, is finally settled to 1000ml with distilled water, obtains Leaf and twig of Common Jasminorange alcohol extract. 4 DEG C of Refrigerator stores, face the used time and mix even.
Embodiment 8
Extract 5ml in Example 7, is settled in the volumetric flask of 10ml with distilled water, to obtain final product. 4 DEG C of Refrigerator stores, face the used time and mix even.
Embodiment 9
A. getting Leaf and twig of Common Jasminorange medicinal material 0.45kg, pulverize and sieve, put in extraction vessel, after being equivalent to the alcohol immersion 1h of concentration expressed in percentage by volume 95% of Leaf and twig of Common Jasminorange weight 8 times, heating and refluxing extraction 2h, filters, and collects filtrate; The alcohol heating reflux that the dregs of a decoction add the concentration expressed in percentage by volume 95% being equivalent to Leaf and twig of Common Jasminorange weight 6 times extracts twice, each 1.5h, filters, and collects filtrate; Merge 3 filtrates, reclaim ethanol to without alcohol taste, obtaining Leaf and twig of Common Jasminorange alcohol extract A.
B. below ethanol extraction A greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ethanol extraction A, in ice cube thaw process, constantly grinding makes ethanol extraction A dissolve and makes suspension; Control suspension temperature, below 10 DEG C, then carries out extraction with sherwood oil and cleans, remove the composition being dissolved in sherwood oil, abandon sherwood oil extraction liquid, and remainder is divided into extract B;
C. extract B ethyl acetate solution is fully extracted, collect extraction liquid, after reclaiming ethyl acetate, obtain ointment. Below ointment greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ointment, in ice cube thaw process, constantly grinding makes ointment dissolve and makes suspension, is finally settled to 1000ml with distilled water, obtains Leaf and twig of Common Jasminorange ethyl acetate extract. 4 DEG C of Refrigerator stores, face the used time and mix even.
Embodiment 10
Extract 5ml in Example 9, is settled in the volumetric flask of 10ml with distilled water, to obtain final product. 4 DEG C of refrigerators keep, and face the used time and mix even.
Embodiment 11
Getting Leaf and twig of Common Jasminorange medicinal material 1kg, pulverize and sieve, put in extraction vessel, the 9L that adds water soaks 1h, heating and refluxing extraction 2h, filters, and collects filtrate. The dregs of a decoction add water 5L, extract 1h; Filter, collect filtrate; The dregs of a decoction add water 5L refluxing extraction 1h again; Filter, collect filtrate. Merge 3 filtrates, it is condensed into medicinal extract, obtains Leaf and twig of Common Jasminorange water extract.
Embodiment 12
Getting Leaf and twig of Common Jasminorange medicinal material 1kg, pulverize and sieve, put in extraction vessel, the 12L that adds water soaks 2h, heating and refluxing extraction 3h, filters, and collects filtrate. The dregs of a decoction add water 8L, extract 1h, filter, and collect filtrate. Merge 2 filtrates, it is condensed into medicinal extract, obtains Leaf and twig of Common Jasminorange water extract.
Embodiment 13
Getting Leaf and twig of Common Jasminorange medicinal material 1kg, pulverize and sieve, put in extraction vessel, after soaking 2h with the ethanol 10L of concentration expressed in percentage by volume 95%, heating and refluxing extraction 2h, filters, and collects filtrate; The dregs of a decoction add the ethanol 5L heating and refluxing extraction 1h of concentration expressed in percentage by volume 95%, filter, and collect filtrate; Merge 2 filtrates, reclaim ethanol to without alcohol taste, being condensed into medicinal extract, obtain Leaf and twig of Common Jasminorange alcohol extract.
Embodiment 14
Getting Leaf and twig of Common Jasminorange medicinal material 1kg, pulverize and sieve, put in extraction vessel, after soaking 3h with the ethanol 12L of concentration expressed in percentage by volume 95%, heating and refluxing extraction 3h, filters, and collects filtrate, reclaims ethanol to without alcohol taste, being condensed into medicinal extract, obtain Leaf and twig of Common Jasminorange alcohol extract.
Experimental example 1: Folium Et Cacumen Murrayae extract is on the impact of normal mouse bile excretion
1 trial-product:
1.1 trial drugs: by reagent: embodiment of the present invention 5-10 prepares gained Leaf and twig of Common Jasminorange aqueous extract, alcohol extract and ethyl acetate extract.
Comparison medicine: bladder of relaxing, lot number 009503214, Shaanxi king pharmaceutcal corporation, Ltd.
1.2 animals: Kunming mouse, male, SPF level, purchased from Guangxi Medical University's Experimental Animal Center, credit number: SCXK osmanthus 2014-0002.
1.3 main instruments: ware balance in FA1004 type, Shanghai Jing Ke balance factory; JY1002 electronic balance, Shanghai Pu Chun measuring instrument company limited, etc.
2 experiment groupings
Blank group: distilled water
Testing 1 group: Leaf and twig of Common Jasminorange aqueous extract high dose group, adopt the extract of the embodiment of the present invention 5, dosage is 4.50g crude drug/kg;
Testing 2 groups: Leaf and twig of Common Jasminorange aqueous extract low dose group, adopt the extract of the embodiment of the present invention 6, dosage is 2.25g crude drug/kg;
Testing 3 groups: Leaf and twig of Common Jasminorange alcohol extract high dose group, adopt the extract of the embodiment of the present invention 7, dosage is 4.50g crude drug/kg;
Testing 4 groups: Leaf and twig of Common Jasminorange alcohol extract low dose group, adopt the extract of the embodiment of the present invention 8, dosage is 2.25g crude drug/kg;
Testing 5 groups: Leaf and twig of Common Jasminorange ethyl acetate extract high dose group, adopt the extract of the embodiment of the present invention 9, dosage is 4.50g crude drug/kg;
Testing 6 groups: Leaf and twig of Common Jasminorange ethyl acetate extract low dose group, adopt the extract of the embodiment of the present invention 10, dosage is 2.25g crude drug/kg;
Comparative example 1 group: bladder of relaxing, 7.76g crude drug/kg.
3 experimental techniques
Get body weight 18��22g mouse 80, it is divided into 8 groups at random, often organize 10, be respectively blank group, test 1 group, test 2 groups, experiment 3, experiment 4, experiment 5, test 6 group and comparative example 1 group. Gastric infusion, 1 time/d, continuous 2d, control group gives to wait volume distilled water. Administration after 2nd day mouse fasting (can't help water) 16h, 1h after medicine, mouse is put to death in dislocation, wins gall-bladder and weighs, puncture gall-bladder and bile is flowed out, weighs after blotting bile with filter paper again. Using the difference of twice, front and back weight as choleresis, calculate choleresis coefficient (mg/10g body weight).
Statistical method data all withRepresent; Adopting SPSS16.0 software to carry out variance analysis, P < 0.05 has statistical significance.
4 results: as shown in table 1.
Table 1 Folium Et Cacumen Murrayae extract on the impact of normal mouse choleresis (excretion) (, n=10)
Compare with blank group: * P < 0.05, * * P < 0.01
Table 1 shows, compare with blank group, Leaf and twig of Common Jasminorange alcohol extract 4.50g crude drug/kg dosage, ethyl acetate extract 4.50,2.25g crude drug/kg dosage can significantly reduce the bile coefficient (promotion bile excretion) of normal mouse, and ethyl acetate extract is more excellent than the effect of alcohol extract.
Experimental example 2: Murraya paniculata (L.) Jack extract is on the impact of ligation bile duct mouse choleresis
1 trial-product:
1.1 trial drugs: by reagent: embodiment of the present invention 5-10 prepares gained Leaf and twig of Common Jasminorange aqueous extract, alcohol extract and ethyl acetate extract. Comparison medicine: bladder of relaxing, lot number 009503214, Shaanxi king pharmaceutcal corporation, Ltd.
1.2 animals: Kunming mouse, male, SPF level, purchased from Guangxi Medical University's Experimental Animal Center, credit number: SCXK osmanthus 2014-0002.
1.3 main instruments: ware balance in FA1004 type, Shanghai Jing Ke balance factory; JY1002 electronic balance, Shanghai Pu Chun measuring instrument company limited, etc.
2 experiment groupings
Blank group: distilled water
Model group: distilled water
Testing 1 group: Leaf and twig of Common Jasminorange aqueous extract high dose group, adopt the extract of the embodiment of the present invention 5, dosage is 4.50g crude drug/kg;
Testing 2 groups: Leaf and twig of Common Jasminorange aqueous extract low dose group, adopt the extract of the embodiment of the present invention 6, dosage is 2.25g crude drug/kg;
Testing 3 groups: Leaf and twig of Common Jasminorange alcohol extract high dose group, adopt the extract of the embodiment of the present invention 7, dosage is 4.50g crude drug/kg;
Testing 4 groups: Leaf and twig of Common Jasminorange alcohol extract low dose group, adopt the extract of the embodiment of the present invention 8, dosage is 2.25g crude drug/kg;
Testing 5 groups: Leaf and twig of Common Jasminorange ethyl acetate extract high dose group, adopt the extract of the embodiment of the present invention 9, dosage is 4.50g crude drug/kg;
Testing 6 groups: Leaf and twig of Common Jasminorange ethyl acetate extract low dose group, adopt the extract of the embodiment of the present invention 10, dosage is 2.25g crude drug/kg;
Comparative example 1 group: bladder of relaxing, 7.76g crude drug/kg.
3 experimental techniques
Get body weight 22��25g mouse 90, it is divided into 9 groups at random, often organize 10, be respectively blank group, model group, experiment 1 group, test 2 group, test 3 groups, test 4 group, test 5 groups, test 6 group and comparative example 1 group. Gastric infusion, 1 time/d, continuous 5d, Normal group and model group such as give at the capacity distilled water. Mouse fasting (can't help water) after 16 hours in 5th day, through etherization, opens abdominal cavity, after ligation bile duct under sword is prominent, sewing up stomach wall, immediately administration, after administration, after 4h, mouse is put to death in dislocation, win gall-bladder and weigh, puncture gall-bladder and bile is flowed out, weigh again after blotting bile with filter paper. Using the difference of twice, front and back weight as choleresis, calculate choleresis coefficient (mg/10g body weight).
Statistical method data all withRepresent; Adopting SPSS16.0 software to carry out variance analysis, P < 0.05 has statistical significance.
4 results: as shown in table 2.
Table 2 Murraya paniculata (L.) Jack extract on the impact of ligation bile duct mouse choleresis (, n=10)
Compare with model group: * P < 0.05, * * P < 0.01
Table 2 result shows, Leaf and twig of Common Jasminorange ethyl acetate extract 4.50g crude drug/kg can significantly increase the bile coefficient (promotion choleresis) of ligation bile duct mouse, and aqueous extract, alcohol extract has the trend increasing ligation bile duct mouse bile coefficient.
Experimental example 3: Folium Et Cacumen Murrayae extract is on the impact of rat bile flow and bile component
1 trial-product:
1.1 trial drugs: by reagent: embodiment of the present invention 7-10 prepares gained Leaf and twig of Common Jasminorange alcohol extract, ethyl acetate extract. Comparison medicine: bladder of relaxing, lot number 009503214, Shaanxi king pharmaceutcal corporation, Ltd.
1.2 animals: SD rat, male, SPF level, is provided by Guangxi Medical University's Experimental Animal Center, animal conformity certification card number: SCXK osmanthus 2014-0002.
1.3 reagent: total cholesterol measures test kit (rCO method), lot number 20150703, Bei Jianxinchuan source, Beijing Bioisystech Co., Ltd; Total bilirubin (T-XZBIL) measures test kit (heavy nitrogen), lot number 2015039, Changchun Hui Li Bioisystech Co., Ltd.
1.4 instruments: the multi-functional microplate reader of ThermoMultiskango all-wave length; EppendorfResearchplus single track pipettor; Venous detaining needle, specifications and models: 24G �� 19mm/Y-G, Weihai Jie Rui Medical Products Co., Ltd.; Ware balance in FA1004 type, Shanghai Jing Ke balance factory; JY1002 electronic balance, Shanghai Pu Chun measuring instrument company limited; Deng.
2 experiment groupings
Blank group: distilled water
Testing 3 groups: Leaf and twig of Common Jasminorange alcohol extract high dose group, adopt the extract of the embodiment of the present invention 7, dosage is 4.50g crude drug/kg;
Testing 4 groups: Leaf and twig of Common Jasminorange alcohol extract low dose group, adopt the extract of the embodiment of the present invention 8, dosage is 2.25g crude drug/kg;
Testing 5 groups: Leaf and twig of Common Jasminorange ethyl acetate extract high dose group, adopt the extract of the embodiment of the present invention 9, dosage is 4.50g crude drug/kg;
Testing 6 groups: Leaf and twig of Common Jasminorange ethyl acetate extract low dose group, adopt the extract of the embodiment of the present invention 10, dosage is 2.25g crude drug/kg;
Comparative example 1 group: bladder of relaxing, 7.76g crude drug/kg.
3 experimental techniques
Get body weight 300��350g male rat 60, it is divided into 6 groups at random, often organize 10, be respectively blank group, test 3 group, test 4 groups, test 5 group, test 6 groups, comparative example 1 group. Gastric infusion, 1 time/d, continuous 3d, blank group gives to wait volume distilled water; Administration after 3rd day rat fasting (can't help water) 16h, after medicine, 30min urethane anaesthetizes (1.25g/kg), faces upward position and is fixed on retaining plate, abdominal cavity is opened along the positive center line of abdomen, finding stomachus pyloricus, upset duodenum, finds the bile duct of white flexible in descendant duodenum mesentery, 2 rhizoid lines are worn under it, ligation pars papillaris, makes " V " shape otch, makes bile duct intubate with venous detaining needle to liver direction, namely there is pistac bile to flow out as seen, collect bile with EP pipe. After stable 20min, collection bile per hour 1 time, totally 3 times. Record each time period bile flow (mL), and measure total cholesterol and total bilirubin content in each time period bile.
4 results: as shown in table 3, table 4, table 5.
Table 3 Folium Et Cacumen Murrayae extract on the impact of rat bile flow (, n=10)
Compare with blank group: * P < 0.05, * * P < 0.01
Table 4 Folium Et Cacumen Murrayae extract on the impact of rat bile total cholesterol level (, n=10)
Compare with control group: * P < 0.05, * * P < 0.01
Table 5 Folium Et Cacumen Murrayae extract on the impact of rat bile total bilirubin content (, n=10)
Compare with control group: * P < 0.05, * * P < 0.01
Table 3 result shows, Leaf and twig of Common Jasminorange alcohol extract 4.50g crude drug/kg dosage and ethyl acetate extract 4.50,2.25g crude drug/kg dosage can significantly increase rat biliary drainage 1h, 2h, 3h; 2.25g crude drug/kg dosage significantly increases the bile output of biliary drainage 2h.
Table 4 result shows, Leaf and twig of Common Jasminorange alcohol extract 4.50g crude drug/kg dosage can significantly reduce rat biliary drainage 1h, 2h, 3h; 2.25g crude drug/kg dosage significantly reduces total cholesterol level in biliary drainage 2h bile. Leaf and twig of Common Jasminorange ethyl acetate extract 4.50g crude drug/kg dosage can significantly reduce rat biliary drainage 1h, 2h, 3h; 2.25g crude drug/kg dosage significantly reduces total cholesterol level in biliary drainage 2h bile.
Table 5 result shows, the total bilirubin content of each time period rat bile is all had no significant effect by Leaf and twig of Common Jasminorange alcohol extract, ethyl acetate extract 4.50,2.25g crude drug/kg dosage.
Conclusion Folium Et Cacumen Murrayae extract is on the impact of normal mouse and ligation bile duct mouse choleresis and excretion and on test-results displays such as the impacts of normal rat bile flow and bile component thereof: Leaf and twig of Common Jasminorange alcohol extract of the present invention, ethyl acetate extract can significantly alleviate the bile coefficient of normal mouse, significantly increase the bile coefficient of ligation bile duct mouse; And can significantly increase the bile output of rat biliary drainage, significantly reduce rat bile total cholesterol level simultaneously. And the Leaf and twig of Common Jasminorange aqueous extract of same dose has the bile coefficient alleviating normal mouse and increases the trend of the bile coefficient of ligation bile duct mouse. Point out Folium Et Cacumen Murrayae extract of the present invention can significantly promote secretion and the excretion of bile, there is choleretic effect. Folium Et Cacumen Murrayae extract choleretic effect order is ethyl acetate extract > alcohol extract > aqueous extract.
Although, above with general explanation, embodiment and test, the present invention is described in detail, but on basis of the present invention, it is possible to it being made some modifications or improvements, this will be apparent to those skilled in the art. Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (8)
1. Leaf and twig of Common Jasminorange is preparing the purposes in Cholagogue drug.
2. Folium Et Cacumen Murrayae extract is preparing the purposes in Cholagogue drug.
3. Leaf and twig of Common Jasminorange ethanol extraction is preparing the purposes in Cholagogue drug.
4. the purposes of the extract of Leaf and twig of Common Jasminorange ethanol extraction after extraction into ethyl acetate in preparation Cholagogue drug.
5. purposes according to claim 4, it is characterised in that, the preparation method of described Leaf and twig of Common Jasminorange acetic acid ethyl ester extract comprises the following steps:
By Leaf and twig of Common Jasminorange medicinal material drying, pulverize, first with extraction using alcohol, reclaim ethanol and concentrate, make alcohol extract; Being cleaned by alcohol extract petroleum ether extraction, remove the composition being dissolved in sherwood oil, the alcohol extract after then being cleaned by petroleum ether extraction by ethyl acetate extracts, and carries out acetic acid ethyl ester extract reclaiming ethyl acetate, and concentrates and be prepared from.
6. purposes according to claim 4, it is characterised in that, the extracting method of described Leaf and twig of Common Jasminorange acetic acid ethyl ester extract comprises the following steps:
A. Leaf and twig of Common Jasminorange medicinal material drying, the alcohol heating reflux being more than 50% by concentration expressed in percentage by volume after pulverizing extracts, and filters, and filtrate recycling ethanol is to without alcohol taste, concentrating to obtain ethanol extraction A;
B. being added water by ethanol extraction A and make suspension, with petroleum ether extraction, abandon sherwood oil liquid, remainder is divided into extract B;
C. extract B being extracted with ethyl acetate, extraction liquid reclaims ethyl acetate and is condensed into cream, obtains Leaf and twig of Common Jasminorange ethyl acetate extract.
7. purposes according to claim 4, it is characterised in that, the extracting method of described Leaf and twig of Common Jasminorange acetic acid ethyl ester extract comprises the following steps:
A. getting Leaf and twig of Common Jasminorange medicinal material drying, after pulverizing, after being equivalent to the alcohol immersion 0.5-2h of Leaf and twig of Common Jasminorange weight 4-12 concentration expressed in percentage by volume 50%-95% doubly, heating and refluxing extraction 1-3h, filters, and collects filtrate; The alcohol heating reflux that the dregs of a decoction add the concentration expressed in percentage by volume 50%-95% being equivalent to Leaf and twig of Common Jasminorange weight 4-8 times extracts twice, each 1-3h, filters, and collects filtrate; Merge 3 filtrates, reclaim ethanol to without alcohol taste, concentrating to obtain ethanol extraction A;
B. below ethanol extraction A greenhouse cooling to 0 DEG C, then adding the frozen water of band ice cube in ethanol extraction A, in ice cube thaw process, constantly grinding makes ethanol extraction A dissolve and makes suspension; Control suspension temperature, below 10 DEG C, then carries out extraction with sherwood oil and cleans, remove the composition being dissolved in sherwood oil, abandon sherwood oil extraction liquid, and remainder is divided into extract B;
C. extract B ethyl acetate solution is fully extracted, collect extraction liquid, reclaim ethyl acetate and be condensed into medicinal extract, obtain described Leaf and twig of Common Jasminorange ethyl acetate extract.
8. as the Folium Et Cacumen Murrayae extract of claim 2-7 as described in any one and pharmaceutically acceptable carrier combination make the purposes of Cholagogue drug aspect.
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| CN115038426B (en) * | 2020-02-03 | 2024-04-26 | 加特弗斯股份公司 | Murraya koenigii extract and its application in cosmetics |
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