CN105588830A - Commercial capillary electrophoresis/chemical luminescence detection combining interface apparatus - Google Patents
Commercial capillary electrophoresis/chemical luminescence detection combining interface apparatus Download PDFInfo
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- CN105588830A CN105588830A CN201410576543.8A CN201410576543A CN105588830A CN 105588830 A CN105588830 A CN 105588830A CN 201410576543 A CN201410576543 A CN 201410576543A CN 105588830 A CN105588830 A CN 105588830A
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Abstract
The invention relates to a chemical detection device and provides a commercial capillary electrophoresis/chemical luminescence detection combining interface apparatus which is characterized by comprising a photon counter. A detection observation window is arranged in the upper surface of the photon counter. A cover shell is arranged above the photon counter. An inner chamber of the cover shell serves as a dark chamber. A four-way unit and a pipe insert device are arranged on the cover shell in a left-right manner. The four-way unit includes a horizontal through hole from the left end to the right end and two vertical holes. A separating capillary is arranged in the horizontal through hole in a passing manner. One end of the separating capillary is stretched out to connect a micrometer caliper and the other end of the separating capillary is stretched into the dark chamber. A reaction capillary is installed on the pipe insert device in a horizontal passing manner. One end of the reaction capillary is stretched into the dark chamber and is connected to the separating capillary, wherein a light transmitting window is arranged at the connection point of the reaction capillary. The other end of the reaction capillary is stretched out to connect a beaker. The apparatus has simple and compact structure, is small in occupied area, is convenient to install and use, is safe and convenient to use, has high detection sensibility, and is mainly used for luminescence property detection of chemical substances.
Description
Technical field
The present invention relates to chemical detection devices, specifically the interface arrangement of a kind of commercialization Capillary Electrophoresis and chemiluminescence detection coupling.
Background technology
Highly sensitive chemiluminescence detection technology is combined with high-resolution micro-isolation technics, is method for separating and analyzing comparatively desirable in current microanalysis. Study from Capillary Electrophoresis and the chemiluminescence detection GC-MS delivered in recent years, quantity of document is in rising trend, demonstrates the development potentiality of chemical luminescence detection method in Micro-Column Separation. Both couplings at present also face many challenges: as the matching of the high voltage in capillary electrophoresis separation system and chemiluminescence detection system; May there is different chemiluminescence reaction dynamics in different component, obtain sometimes nonlinear curved line relation etc. These thorny problems have proposed very high requirement to combined apparatus.
In order to obtain desirable separating effect and higher sensitivity, the factor of first considering is exactly the mixed mode of determinand and chemiluminescence detection reagent and detects volume. In addition, in order to obtain stable analytic signal, must consider for separating of buffer solution and the compatibility of chemical illuminating reagent pH value and the stability of fluid-mixing. Still can keep good isolating environment and flow pattern at surveyed area, generally mixed mode after first-selected post in order to ensure component after separating.
Capillary Electrophoresis generally needs design interface device fully to mix with liquid after realizing chemical illuminating reagent, capillary electrophoresis column with chemiluminescence detection coupling, utilize chemiluminescent enhancing or abated effect to detect, reasonably interface arrangement plays key effect to the stability of coupling, there is no at present commercial Capillary Electrophoresis and chemiluminescence detection combined system. In prior art, be that the luminescent properties that adopts various existing instruments to piece together chemical substance in experiment detects, the problem of existence is: inconvenient operation, accuracy of detection is lower.
Summary of the invention
Object of the present invention will solve exactly prior art and detect chemical substance luminescent properties inconvenient operation, the problem that accuracy of detection is low, the interface arrangement of a kind of commercialization Capillary Electrophoresis and chemiluminescence detection coupling is provided, thereby realizes high efficiency and the highly sensitive perfect adaptation of chemiluminescence detection of capillary electrophoresis separation.
Concrete scheme of the present invention is: the interface arrangement of a kind of commercialization Capillary Electrophoresis and chemiluminescence detection coupling, it is characterized in that: there is a photon counter, its upper surface is provided with detection observation window, one case is housed above photon counter, in case, die cavity is darkroom, four-way and intubator that left and right is arranged are housed on case, described four-way includes a horizontal through hole and connects two ends, left and right and two upright openings, two upright opening lower ends are communicated with horizontal through hole, in horizontal through hole, be coated with a separation capillary, separation capillary one end tipping micrometer caliper that stretches out, the other end stretches in darkroom, on described intubator, be coated with horizontally disposed reaction capillary, reaction capillary and separation capillary are coaxially arranged, reacting one end capillaceous stretches in darkroom and docks with separation capillary, and be provided with light inlet window at reaction capillary joint, react the other end capillaceous tipping beaker that stretches out.
Four-way described in the present invention is to adopt polyetheretherketonematerials materials to make.
Described in the present invention, in four-way, the upper design of one of them upright opening is gravity level tank.
Micrometer caliper described in the present invention arranges apart from the port 200mm place of horizontal through hole place of four-way.
The light inlet window reacting described in the present invention on capillary is just arranged the detection observation window of photon counter.
The external diameter of separation capillary described in the present invention is less than or equal to reaction capillary inner diameter, and separation capillary is to insert in reaction capillary to dock.
In the time of work, detected material is by commercialization HPCE sample introduction, luminous after mixing with luminescence reagent at detection window place, luminous signal detects by photon counter, described photon counter good stability, the range of linearity is wide, antijamming capability is strong, detection sensitivity is higher, improves and has better flexibility, better tightness for the sensitivity of different chemical luminescence system, effectively lucifuge, reduces the pollution of veiling glare. In the gravity level tank building in four-way, by controlling the liquid level of chemical luminescence for liquid in gravity level tank, flow velocity after the automatic adjustable column of employing gravity mode, this design requires and ensures that reproducibility of analysis results effect is remarkable meeting chemiluminescence detection.
Interface arrangement of the present invention is simple and compact for structure, takes up room little, is convenient to install and use, and whole system ground connection is good, and handling safety, facility, detection sensitivity is high, accuracy of detection is high.
Brief description of the drawings
Fig. 1 is general structure schematic diagram of the present invention.
In figure: 1-micrometer caliper, 2-four-way, 3-gravity level tank, 4-upright opening, 5-case, 6-darkroom, 7-light inlet window, 8-intubator, 9-reaction capillary, 10-beaker, 11-photon counter, 12-detection observation window, 13-horizontal through hole, 14-separation capillary.
Detailed description of the invention
Referring to Fig. 1, the present invention has a photon counter 11, its upper surface is provided with and detects observation window 12, one case 5 is housed above photon counter 11, the interior die cavity of case 5 is darkroom 6, four-way 2 and intubator 8 that left and right is arranged are housed on case 5, described four-way 2 includes a horizontal through hole 13, connect two ends, left and right and two upright openings 4, two upright opening 4 lower ends are communicated with horizontal through hole 13, in horizontal through hole 13, be coated with a separation capillary 14, separation capillary 14 one end stretch out and are hinged with micrometer caliper 1, and the other end stretches in darkroom 6; On described intubator 8, be coated with horizontally disposed reaction capillary 9, reaction capillary 9 is coaxially arranged with separation capillary 14, one end of reaction capillary 9 is stretched in darkroom 6 and is docked with separation capillary 14, and be provided with at reaction capillary 9 joints the coating that light inlet window 7(removes reaction capillary surface and can obtain light inlet window), the other end of the reaction capillary 9 tipping beaker 10 that stretches out.
Four-way 2 described in the present embodiment is to adopt polyetheretherketonematerials materials to make.
In four-way 2 described in the present embodiment, wherein the upper design in left vertical hole 4 is gravity level tank 3.
Micrometer caliper 1 described in the present embodiment arranges apart from the horizontal through hole 13 port 200mm places of place of four-way 2.
The light inlet window 7 reacting described in the present embodiment on capillary 9 is just arranged the detection observation window 12 of photon counter 11.
The external diameter of separation capillary 14 described in the present embodiment is less than or equal to reaction capillary 9 internal diameters, and separation capillary 14 is to insert in reaction capillary 9 to dock.
Claims (6)
1. the interface arrangement of a commercialization Capillary Electrophoresis and chemiluminescence detection coupling, it is characterized in that: there is a photon counter, its upper surface is provided with detection observation window, one case is housed above photon counter, in case, die cavity is darkroom, four-way and intubator that left and right is arranged are housed on case, described four-way includes a horizontal through hole and connects two ends, left and right and two upright openings, two upright opening lower ends are communicated with horizontal through hole, in horizontal through hole, be coated with a separation capillary, separation capillary one end tipping micrometer caliper that stretches out, the other end stretches in darkroom, on described intubator, be coated with horizontally disposed reaction capillary, reaction capillary and separation capillary are coaxially arranged, reacting one end capillaceous stretches in darkroom and docks with separation capillary, and be provided with light inlet window at reaction capillary joint, react the other end capillaceous tipping beaker that stretches out.
2. the interface arrangement of a kind of commercialization Capillary Electrophoresis according to claim 1 and chemiluminescence detection coupling, is characterized in that: described four-way is to adopt polyetheretherketonematerials materials to make.
3. the interface arrangement of a kind of commercialization Capillary Electrophoresis according to claim 1 and 2 and chemiluminescence detection coupling, is characterized in that: in described four-way, the upper design of one of them upright opening is gravity level tank.
4. the interface arrangement of a kind of commercialization Capillary Electrophoresis according to claim 1 and chemiluminescence detection coupling, is characterized in that: described micrometer caliper arranges apart from the horizontal through hole external port 200mm place of four-way.
5. the interface arrangement of a kind of commercialization Capillary Electrophoresis according to claim 1 and chemiluminescence detection coupling, is characterized in that: the light inlet window on described reaction capillary is just arranged the detection observation window of photon counter.
6. the interface arrangement of a kind of commercialization Capillary Electrophoresis according to claim 1 and chemiluminescence detection coupling, it is characterized in that: described separation capillary external diameter is less than or equal to reaction capillary inner diameter, separation capillary is to insert in reaction capillary to dock.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410576543.8A CN105588830A (en) | 2014-10-24 | 2014-10-24 | Commercial capillary electrophoresis/chemical luminescence detection combining interface apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410576543.8A CN105588830A (en) | 2014-10-24 | 2014-10-24 | Commercial capillary electrophoresis/chemical luminescence detection combining interface apparatus |
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| Publication Number | Publication Date |
|---|---|
| CN105588830A true CN105588830A (en) | 2016-05-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410576543.8A Pending CN105588830A (en) | 2014-10-24 | 2014-10-24 | Commercial capillary electrophoresis/chemical luminescence detection combining interface apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085229A (en) * | 2015-05-13 | 2018-12-25 | 爱科来株式会社 | Analysis tool and analysis system |
| CN111678929A (en) * | 2020-06-18 | 2020-09-18 | 中国科学院上海高等研究院 | Synchrotron radiation serial crystallography electrostatic spinning sample loading device and synchrotron radiation-based serial crystallography experimental method |
-
2014
- 2014-10-24 CN CN201410576543.8A patent/CN105588830A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085229A (en) * | 2015-05-13 | 2018-12-25 | 爱科来株式会社 | Analysis tool and analysis system |
| CN111678929A (en) * | 2020-06-18 | 2020-09-18 | 中国科学院上海高等研究院 | Synchrotron radiation serial crystallography electrostatic spinning sample loading device and synchrotron radiation-based serial crystallography experimental method |
| CN111678929B (en) * | 2020-06-18 | 2023-08-25 | 中国科学院上海高等研究院 | Synchronous radiation serial crystallography electrostatic spinning sample loading device and serial crystallography experimental method based on synchronous radiation |
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Application publication date: 20160518 |
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| WD01 | Invention patent application deemed withdrawn after publication |