CN105567577A - Trichoderma atroviride strain and application thereof - Google Patents
Trichoderma atroviride strain and application thereof Download PDFInfo
- Publication number
- CN105567577A CN105567577A CN201610063485.8A CN201610063485A CN105567577A CN 105567577 A CN105567577 A CN 105567577A CN 201610063485 A CN201610063485 A CN 201610063485A CN 105567577 A CN105567577 A CN 105567577A
- Authority
- CN
- China
- Prior art keywords
- tobacco
- trichoderma atroviride
- pectin
- strain
- mierocrystalline cellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a trichoderma atroviride strain and application thereof to tobacco. The strain is a trichoderma atroviride C9-13 strain, and the preservation number is CGMCC No.8077. The strain can degrade pectin and cellulose at the same time. A method for applying the trichoderma atroviride C9-13 strain to treating the tobacco comprises the steps that trichoderma atroviride C9-13 is firstly taken to be cultured, then a spore suspension is prepared, then the spore suspension is added to a fermentation medium to be cultured, fermented supernatant fluid is prepared, and finally the fermented supernatant fluid is sprayed to the surface of the tobacco. The content of pectin and cellulose of the treated tobacco is obviously reduced, smoking quality, especially the fragrance amount is improved to a certain degree, and the chemical ingredient harmony is also improved.
Description
Technical field
The invention belongs to fermentable and Application Areas thereof, particularly relate to a kind of Trichoderma atroviride strain and application thereof.
Background technology
Tobacco cell wall material is made up of Mierocrystalline cellulose, hemicellulose, xylogen, pectin substance and some mineral elements, accounts for 26% ~ 35% of amount of dry matter in cured tobacco leaf, plays and maintains cyto-architectural effect, have larger impact to tobacco interior quality.Cell wall substance content is higher, and quality of tobacco is poorer.In discarded tobacco leaf reducing sugar and Soluble adhesion molecule lower, Mierocrystalline cellulose, hemicellulose, xylogen and pectin content are higher, cause discarded tobacco leaf have strong impulse taste, blue foreign smell heavy, inhale pungent, the puckery mouth of taste, the fragrance of tobacco leaf can not be appeared.In cell wall substance, pectin substance and Mierocrystalline cellulose not only affect water absorbability and the incendivity of tobacco leaf, are also the main producers things of the objectionable constituent such as phenolic compound in smoke and small molecule aldehyde compound; Pectin substance in reduction tobacco leaf and content of cellulose can not only improve quality of tobacco, and can reduce the release of harmful ingredients in flue gas.Pectin substance has certain effect to the water absorbability of tobacco leaf and elasticity, but content causes the increase of tar growing amount compared with Gao Shihui, and produces the objectionable constituent such as methyl alcohol, formaldehyde burning and sucking in process, unfavorable to quality of tobacco.The result of study of Schlotzhauer shows, monohydroxy volatile phenol (cresols, the xylenol) composition in flue gas generates primarily of Mierocrystalline cellulose thermo-cracking in cigarette burning process.
At present, usually adopt two kinds of approach to come pectin substance in degrading tobacco and Mierocrystalline cellulose, one is apply commercial polygalacturonase or cellulase, and two is that directly application can produce microorganism and the fermented liquid of polygalacturonase or cellulase.As far back as 1973, the people such as the Gravely of B & W tobacco company just once utilized Erwinia carotovora Erwiniacarotovora to the pectin of degrading in tobacco leaf, to substitute the offal granularity in some Machining Technology reduction thin slice preparation process, and retain the natural fragrance fragrance in tobacco.He can produce again the carrot soft rot Erwinia of polygalacturonase and can produce the fungus Trichoderma Trichodermalongibrachiatum mixed culture of cellulase subsequently, for pectin substance in offal and the Mierocrystalline cellulose of degrading.
Domestic Scientific Research personnel have also carried out some researchs in the pectin substance utilized in microbiological deterioration tobacco or Mierocrystalline cellulose.Deng state guest etc. on redrying aging tobacco leaves separation screening to can the fungi of depolymerized pectin matter, a strain higher for wherein inulinase-producing activity is fermented, gained crude enzyme liquid process upper tobacco leaf, after ferment treatment, tobacco leaf cells wall material and pectin content all decrease, and smoking quality improves.But studies in China mainly concentrates on the single depolymerized pectin of screening or cellulosic bacterial strain aspect, there is not yet the report screening depolymerized pectin and Mierocrystalline cellulose function stem simultaneously.
Summary of the invention
The present invention is based on the deficiency of background technology and a kind of depolymerized pectin and cellulosic function stem are simultaneously provided, extract this function stem fermented liquid, spray in pipe tobacco or stem surface, while reducing pectin and content of cellulose, chemical composition Harmony also has certain improvement.
For solving pectin substance in simultaneously degrading tobacco and cellulosic problem, the invention provides a kind of Trichoderma atroviride strain and this Trichoderma atroviride strain for degrading tobacco pectin substance and cellulosic method.
The object of the invention is to be achieved through the following technical solutions: a kind of Trichoderma atroviride strain, described bacterial strain is Trichoderma atroviride (Trichodermaatroviride) C9-13, within 08 month 27 days, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC of No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City in 2013, preserving number is CGMCCNo.8077.
Trichoderma atroviride C9-13 provided by the invention is a kind of energy depolymerized pectin and cellulosic function stem simultaneously.
The bacterial strain that the present invention relates to grows rapidly on potato dextrose agar, and radially to surrounding expansion, be namely paved with whole flat board after cultivating 3d, mycelia is white fluffy, fine and close, pros and cons solid colour, and colony characteristics is shown in Fig. 1; Colony edge is main Chan Bao district, and cultivating 10d Hou Chanbao district is deep green, and the bacterium colony back side is pistac.Mycelium has tabula, and conidiophore is arborization, and branches end is stigma, and conidiophore is shown in Fig. 2, and stigma produces conidium, conidium ellipse or oval.The ITS sequence of this bacterial strain is the principal character foundation of this bacterial strain of qualification, and its ITS sequence is shown in the SEQIDNO.1 in Fig. 3 and sequence table, and the bacterial strain phylogenetic tree built according to ITS sequence is shown in Fig. 4.
By Trichoderma atroviride C9-13 streak inoculation on potato dextrose agar, cultivate 48 ~ 72h for 28 DEG C, add sterilized water vibration and obtain spore suspension (spore concentration is 10
6individual/mL), be inoculated in fermention medium with the inoculum size of 5 ~ 30% (V/V), 25 ~ 28 DEG C, 100 ~ 200r/min cultivates 48 ~ 72h, in 3000 ~ 6000r/min, centrifugal 5 ~ 10min at 4 ~ 10 DEG C, can fermented supernatant fluid be obtained.
Described fermention medium comprises: wheat stalk powder 5 ~ 30g/L, (NH
4)
2sO
40.5 ~ 2.0g/L, urea 0.1 ~ 0.5g/L, peptone 0.5 ~ 2.0g/L, MgSO
47H
2o0.1 ~ 0.5g/L, CaCl
20.1 ~ 0.3g/L.
Trichoderma atroviride C9-13 fermented supernatant fluid is sprayed in pipe tobacco or stem surface by the ratio uniform of 5 ~ 30% (W/W) or 10-30% (W/W) or 15-30% (W/W), measure the pectin of the rear sample of process, Mierocrystalline cellulose and routine chemical components, result shows that Trichoderma atroviride C9-13 fermented liquid can reduce pectin and content of cellulose, and improves tobacco leaf sucking quality.
Invention also provides the method for a kind of Trichoderma atroviride strain for degrading tobacco Mierocrystalline cellulose and pectin, comprise the following steps:
Step 1) by the streak inoculation of Trichoderma atroviride C9-13 bacterial strain on potato dextrose agar, 28 DEG C cultivate 48 ~ 72h, then add sterilized water vibration obtain spore suspension;
Step 2) by spore suspension with 5 ~ 30% inoculum size be inoculated in fermention medium, on constant-temperature table cultivate 48 ~ 72h, shaking speed is 100 ~ 200r/min, and temperature is 25 ~ 28 DEG C; Again by fermentation culture based on 3000 ~ 6000r/min, centrifugal 5 ~ 10min at 4 ~ 10 DEG C, namely obtain fermented supernatant fluid;
Step 3) fermented supernatant fluid of 5 ~ 30 weight parts or 10-30 weight part or 15-30 weight part evenly sprayed tobacco surface in 100 weight parts, then by tobacco plastic bag sealing, be put in baking oven and process (baking) 6h under 50 DEG C of conditions.
Further improvement, described step 2) in, by spore suspension with 10% inoculum size transfer in fermention medium, constant-temperature table cultivates 72h, and shaking speed is 170r/min, and temperature is 25 DEG C; Again by fermentation culture based on 6000r/min, centrifugal 5min at 4 DEG C.
Further improvement, described step 2) in, fermention medium is comprise wheat stalk powder 5 ~ 30g, ammonium sulfate 0.5 ~ 2.0g, urea 0.1 ~ 0.5g, peptone 0.5 ~ 2.0g, bitter salt 0.1 ~ 0.5g and calcium chloride 0.1 ~ 0.3g in every premium on currency.
Further improvement, described step 2) in, fermention medium is comprise wheat stalk powder 10g, ammonium sulfate 1g, urea 0.2g, peptone 1g, bitter salt 0.1g and calcium chloride 0.1g in every premium on currency.
Compared with prior art, the present invention has the following advantages:
1. Trichoderma atroviride provided by the invention can be degraded Mierocrystalline cellulose in tobacco leaf and pectin effectively, improves quality of tobacco, strengthens water absorbability and incendivity.
2. use method process tobacco of the present invention to improve perfume quantity, promote sucking quality, chemical composition Harmony is also improved.
3. use the method in the present invention can to degrade Mierocrystalline cellulose in tobacco leaf and pectin simultaneously, can enhance productivity.
Accompanying drawing explanation
Fig. 1 is bacterial strain C9-13 colonial morphology;
Fig. 2 is bacterial strain C9-13 conidiophore form;
Fig. 3 is bacterial strain C9-13ITS sequence;
Fig. 4 is bacterial strain C9-13 phylogenetic tree.
Specific embodiment
In following examples, be C3F grade recipe cut tobacco for examination tobacco sample, be specially the Qujing of Yunnan in (each 500g) 2012 years of equivalent and the C3F grade tobacco leaf of Chenzhou, Hunan Province, after chopping, mixture is even.
The preparation method of the potato dextrose agar in following examples is: the potato of getting 200g cleans peeling, and be cut into small pieces, add water well-done, by eight layers of filtered through gauze, heating, add agar 15g, continue heated and stirred mixing, after agar has dissolved, add glucose 20g, stir, slightly supply moisture to 1000 milliliter again, packing test tube or Erlenmeyer flask after cooling, jump a queue, wrap up, take out test tube pendulum inclined-plane at 120 DEG C after sterilizing about 20 minutes or shake up, after cooling, storage is for subsequent use.
Embodiment 1
Step 1) by Trichoderma atroviride C9-13 streak inoculation on potato dextrose agar, 28 DEG C cultivate 72h, then add sterilized water vibration obtain spore suspension, spore concentration is 10
6individual/mL;
Step 2) by spore suspension with 10% inoculum size be inoculated in fermention medium, constant-temperature table cultivates 72h, and shaking speed is 170r/min, and temperature is 25 DEG C; Again by fermentation culture based on 6000r/min, centrifugal 5min at 4 DEG C, namely obtain fermented supernatant fluid;
Wherein fermention medium is comprise wheat stalk powder 10g, ammonium sulfate 1g, urea 0.2g, peptone 1g, bitter salt 0.1g and calcium chloride 0.1g in every premium on currency.
Step 3) fermented supernatant fluid of 15 weight parts evenly sprayed tobacco surface in 100 weight parts, then by tobacco plastic bag sealing, be put in baking oven and process 6h under 50 DEG C of conditions.Terminating rear 80 DEG C of process 20min makes enzyme lose activity, and then measures pectin, Mierocrystalline cellulose and routine chemical components content respectively.Sample is rolled into single-tobacco-typed cigarette simultaneously, sensory evaluation is carried out to it, respectively qualitative evaluation is carried out to aroma quality, perfume quantity, concentration, assorted gas, pungency, pleasant impression and strength 7 indexs.Model pectin and content of cellulose are in table 1, and routine chemical components is in table 2, and sensible quality is in table 3
As can be seen from Table 1, after Trichoderma atroviride C9-13 process cut tobacco 6h, pectin content decline 14.53%, content of cellulose decline 6.80%, this function stem to pectin and cellulosic degradation effect better.As can be seen from Table 2, after fermentation liquor treatment, cut tobacco reducing sugar, total sugar content increase.As can be seen from Table 3, after fermentation liquor treatment, sample perfume quantity increases to some extent, and processing sample sense organ oeverall quality is better than control sample.
Before and after table 1 fermentation liquor treatment, pectin and content of cellulose compare
Routine chemical components change before and after table 2 fermentation liquor treatment
Before and after table 3 fermentation liquor treatment, cut tobacco sensible quality compares
Embodiment 2
Step 1) by Trichoderma atroviride C9-13 streak inoculation on potato dextrose agar, 28 DEG C cultivate 48h, then add sterilized water vibration obtain spore suspension;
Step 2) by spore suspension with 30% inoculum size be inoculated in fermention medium, constant-temperature table cultivates 48h, and shaking speed is 100r/min, and temperature is 28 DEG C; Again by fermentation culture based on 3000r/min, centrifugal 10min at 10 DEG C, namely obtain fermented supernatant fluid;
Described fermention medium is comprise wheat stalk powder 5g, ammonium sulfate 2.0g, urea 0.1g, peptone 2.0g, bitter salt 0.5g and calcium chloride 0.3g in every premium on currency;
Step 3) fermented supernatant fluid of 5 weight parts evenly sprayed tobacco surface in 100 weight parts, then by tobacco plastic bag sealing, be put in baking oven and process 6h under 50 DEG C of conditions.Terminating rear 80 DEG C of process 20min makes enzyme lose activity, and then measures pectin, Mierocrystalline cellulose and routine chemical components content respectively.Sample is rolled into single-tobacco-typed cigarette simultaneously, sensory evaluation is carried out to it, respectively qualitative evaluation is carried out to aroma quality, perfume quantity, concentration, assorted gas, pungency, pleasant impression and strength 7 indexs.Model pectin and content of cellulose are in table 4, and routine chemical components is in table 5, and sensible quality is in table 6.
As can be seen from Table 4, after Trichoderma atroviride C9-13 process cut tobacco 6h, pectin content declines 1.62%, and content of cellulose declines 2.52%, and this function stem have certain degradation effect to pectin and fiber.As can be seen from Table 5, after fermentation liquor treatment, cut tobacco reducing sugar, total sugar content slightly increase.As can be seen from Table 6, after fermentation liquor treatment, assorted gas slightly improves, and processing sample sense organ oeverall quality is slightly better than control sample.
Before and after table 4 fermentation liquor treatment, pectin and content of cellulose compare
Routine chemical components change before and after table 5 fermentation liquor treatment
Before and after table 6 fermentation liquor treatment, cut tobacco sensible quality compares
Embodiment 3
Step 1) by Trichoderma atroviride C9-13 streak inoculation on potato dextrose agar, 28 DEG C cultivate 48 ~ 72h, then add sterilized water vibration obtain spore suspension;
Step 2) by spore suspension with 5% inoculum size be inoculated in fermention medium, constant-temperature table cultivates 56h, and shaking speed is 200r/min, and temperature is 25 DEG C; Again by fermentation culture based on 4500r/min, centrifugal 8min at 8 DEG C, namely obtain fermented supernatant fluid;
Described fermention medium is comprise wheat stalk powder 30g, ammonium sulfate 0.5g, urea 0.5g, peptone 0.5g, bitter salt 0.1g and calcium chloride 0.1g in every premium on currency.
Step 3) fermented supernatant fluid of 30 weight parts evenly sprayed tobacco surface in 100 weight parts, then by tobacco plastic bag sealing, be put in baking oven and process 6h under 50 DEG C of conditions.Terminating rear 80 DEG C of process 20min makes enzyme lose activity, and then measures pectin, Mierocrystalline cellulose and routine chemical components content respectively.Sample is rolled into single-tobacco-typed cigarette simultaneously, sensory evaluation is carried out to it, respectively qualitative evaluation is carried out to aroma quality, perfume quantity, concentration, assorted gas, pungency, pleasant impression and strength 7 indexs.Model pectin and content of cellulose are in table 7, and routine chemical components is in table 8, and sensible quality is in table 9.
As can be seen from Table 7, after Trichoderma atroviride C9-13 process cut tobacco 6h, pectin content decline 16.97%, content of cellulose decline 10.81%, this function stem to pectin and cellulosic degradation effect better.As can be seen from Table 8, after fermentation liquor treatment, cut tobacco reducing sugar, total sugar content increase.As can be seen from Table 9, after fermentation liquor treatment, sample perfume quantity increases to some extent, although the index such as perfume quantity, concentration score value there is no and significantly promotes, but processing sample sense organ oeverall quality is better than control sample, after fermentation liquor treatment is described, sample sensible quality has certain lifting.
Before and after table 7 fermentation liquor treatment, pectin and content of cellulose compare
Routine chemical components change before and after table 8 fermentation liquor treatment
Before and after table 9 fermentation liquor treatment, cut tobacco sensible quality compares
Above embodiment only for illustration of the present invention, but is not used for limiting the scope of the invention, every following instance is done according to technical spirit of the present invention any simple modification, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Sequence table
Claims (12)
1. a Trichoderma atroviride strain, it is characterized in that, described bacterial strain is Trichoderma atroviride (Trichodermaatroviride) C9-13, be deposited in China Committee for Culture Collection of Microorganisms of Pekinese common micro-organisms center on 08 27th, 2013, preserving number is CGMCCNo.8077.
2. Trichoderma atroviride strain as claimed in claim 1, is characterized in that, energy is degraded cellulose and pectin simultaneously.
3. Trichoderma atroviride strain as claimed in claim 1, it is characterized in that, this bacterial strain grows rapidly on potato dextrose agar, radially to surrounding expansion, be namely paved with whole flat board after cultivating 3d, mycelia is white fluffy, fine and close, pros and cons solid colour, cultivating 10d Hou Chanbao district is deep green, and the bacterium colony back side is pistac.
4. a kind of Trichoderma atroviride strain as claimed in claim 1, is characterized in that, the fermention medium of bacterial strain comprises: wheat stalk powder 5 ~ 30g/L, (NH
4)
2sO
40.5 ~ 2.0g/L, urea 0.1 ~ 0.5g/L, peptone 0.5 ~ 2.0g/L, MgSO
47H
2o0.1 ~ 0.5g/L, CaCl
20.1 ~ 0.3g/L.
5. the application of Trichoderma atroviride strain in degrading tobacco Mierocrystalline cellulose and pectin as claimed in claim 1.
6. the application of Trichoderma atroviride strain in degrading tobacco Mierocrystalline cellulose and pectin as claimed in claim 5, it is characterized in that: the fermented supernatant fluid of described bacterial strain is evenly sprayed in tobacco surface, effectively can reduce pectin and cellulosic content in tobacco, tobacco leaf sucking quality can also be improved simultaneously.
7. Trichoderma atroviride strain as claimed in claim 1 is used for the method for degrading tobacco Mierocrystalline cellulose and pectin, it is characterized in that, comprises the following steps:
Step 1) by the streak inoculation of Trichoderma atroviride C9-13 bacterial strain on potato dextrose agar, 28 DEG C cultivate 48 ~ 72h, then add sterilized water vibration obtain spore suspension;
Step 2) by spore suspension with 5 ~ 30% inoculum size be inoculated in fermention medium, on constant-temperature table cultivate 48 ~ 72h, shaking speed is 100 ~ 200r/min, and temperature is 25 ~ 28 DEG C; Again by fermentation culture based on 3000 ~ 6000r/min, centrifugal 5 ~ 10min at 4 ~ 10 DEG C, namely obtain fermented supernatant fluid;
Step 3) fermented supernatant fluid of 5 ~ 30 weight parts evenly sprayed tobacco surface in 100 weight parts, then by tobacco plastic bag sealing, be put in baking oven, under 50 DEG C of conditions, process 6h.
8. a kind of Trichoderma atroviride strain as claimed in claim 7 is used for the method for degrading tobacco Mierocrystalline cellulose and pectin, it is characterized in that, described step 2) in, by spore suspension with 10% inoculum size transfer in fermention medium, constant-temperature table cultivates 72h, shaking speed is 170r/min, and temperature is 25 DEG C; Again by fermentation culture based on 6000r/min, centrifugal 5min at 4 DEG C.
9. a kind of Trichoderma atroviride strain as claimed in claim 7 is used for the method for degrading tobacco Mierocrystalline cellulose and pectin, it is characterized in that, the preparation method of described potato dextrose agar is: the potato taking 200g cleans peeling, and be cut into small pieces, add water well-done, by eight layers of filtered through gauze, heating, add agar 15-20g, continue heated and stirred mixing, after agar has dissolved, add glucose 20g, stir, slightly supply moisture to 1000 milliliter again after cooling, packing test tube or Erlenmeyer flask, jump a queue, wrapping, take out test tube pendulum inclined-plane at 120 DEG C after sterilizing about 20 minutes or shake up, after cooling, storage is for subsequent use.
10. a kind of Trichoderma atroviride strain as claimed in claim 7 is used for the method for degrading tobacco Mierocrystalline cellulose and pectin, it is characterized in that, described step 2) in, fermention medium is comprise wheat stalk powder 5 ~ 30g, ammonium sulfate 0.5 ~ 2.0g, urea 0.1 ~ 0.5g, peptone 0.5 ~ 2.0g, bitter salt 0.1 ~ 0.5g and calcium chloride 0.1 ~ 0.3g in every premium on currency.
11. a kind of Trichoderma atroviride strain as claimed in claim 10 are used for the method for degrading tobacco Mierocrystalline cellulose and pectin, it is characterized in that, described step 2) in, described fermention medium is comprise wheat stalk powder 10g, ammonium sulfate 1g, urea 0.2g, peptone 1g, bitter salt 0.1g and calcium chloride 0.1g in every premium on currency.
12. a kind of Trichoderma atroviride strain as claimed in claim 7 are used for the method for degrading tobacco Mierocrystalline cellulose and pectin, it is characterized in that, described step 2) in, by spore suspension with 10% inoculum size transfer in fermention medium, constant-temperature table cultivates 72h, shaking speed is 170r/min, and temperature is 25 DEG C; Again by fermentation culture based on 6000r/min, centrifugal 5min at 4 DEG C; Described fermention medium is comprise wheat stalk powder 10g, ammonium sulfate 1g, urea 0.2g, peptone 1g, bitter salt 0.1g and calcium chloride 0.1g in every premium on currency; The preparation method of described potato dextrose agar is: the potato taking 200g cleans peeling, and is cut into small pieces, and adds water well-done, by eight layers of filtered through gauze, heating, adds agar 15-20g, continue heated and stirred mixing, after agar has dissolved, add glucose 20g, stir, slightly supply moisture to 1000 milliliter again, packing test tube or Erlenmeyer flask after cooling, jump a queue, wrap up, take out test tube pendulum inclined-plane at 120 DEG C after sterilizing about 20 minutes or shake up, after cooling, storage is for subsequent use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610063485.8A CN105567577B (en) | 2016-01-29 | 2016-01-29 | A kind of Trichoderma atroviride strain and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610063485.8A CN105567577B (en) | 2016-01-29 | 2016-01-29 | A kind of Trichoderma atroviride strain and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105567577A true CN105567577A (en) | 2016-05-11 |
| CN105567577B CN105567577B (en) | 2019-08-23 |
Family
ID=55878212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610063485.8A Active CN105567577B (en) | 2016-01-29 | 2016-01-29 | A kind of Trichoderma atroviride strain and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105567577B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058127A (en) * | 2017-03-30 | 2017-08-18 | 周口师范学院 | A kind of Trichoderma fermentation substrate and application based on monosodium glutamate waste liquid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925365A (en) * | 2012-10-24 | 2013-02-13 | 郑州大学 | Trichoderma atroviride strain and application thereof in preparation of cellulase |
-
2016
- 2016-01-29 CN CN201610063485.8A patent/CN105567577B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925365A (en) * | 2012-10-24 | 2013-02-13 | 郑州大学 | Trichoderma atroviride strain and application thereof in preparation of cellulase |
Non-Patent Citations (2)
| Title |
|---|
| 徐春厚: "绿色木霉X-13 株固态发酵产酶优化条件的研究", 《云南农业大学学报》 * |
| 颜秋生等: "纤维素酶制剂活力的测定方法", 《遗传》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058127A (en) * | 2017-03-30 | 2017-08-18 | 周口师范学院 | A kind of Trichoderma fermentation substrate and application based on monosodium glutamate waste liquid |
| CN107058127B (en) * | 2017-03-30 | 2020-09-08 | 周口师范学院 | Trichoderma fermentation substrate based on monosodium glutamate waste liquid and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105567577B (en) | 2019-08-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106833898B (en) | Preparation method of rice-flavor tobacco flavor | |
| CN103202533B (en) | Complex microbial preparation for quickening tobacco aging and improving tobacco quality and application thereof | |
| CN102250812A (en) | Bacillus subtilis preparation for tobacco stem treatment | |
| CN105907649A (en) | Fungus for degrading pectin and cellulose in tobacco stems and application of fungus | |
| Yao et al. | Screening of Cellulase-producing Bacteria and their Effect on the Chemical Composition and Aroma Quality Improvement of Cigar Wrapper Leaves. | |
| CN109090697B (en) | Method for fermenting tobacco by mixed bacteria | |
| CN102399725B (en) | Cigarette group imparting strain, compound microbial preparation and application thereof | |
| CN103451106A (en) | Microbial preparation for tobacco stem treatment and preparation method thereof | |
| CN106434359A (en) | Trichoderma aureoviride strain and method for preparing fig cigarette flavor with same | |
| CN103451108A (en) | Aureobasidium pullulans OF-01 strain and method for preparing endogenous derived flavor OF tobacco by using same | |
| CN105559136A (en) | Method for preparing electronic cigarette juice fragrance from green tea enzyme | |
| CN105176679A (en) | Cigarette essence containing lucid ganoderma fermentation broth and preparation method thereof | |
| CN110169587B (en) | Application method of enzyme preparation for producing vermilion-like mature spots after tobacco leaf baking | |
| CN105567577A (en) | Trichoderma atroviride strain and application thereof | |
| CN103194399B (en) | Microorganism strain for generating pectinase and application thereof in tobacco | |
| CN106676013B (en) | Method for improving quality of redried tobacco leaves by fermenting golden flower fungus tea leaves | |
| CN105713847A (en) | Trichoderma aureoviride strain and method for preparing notoginseng bud cigarette fragrance by utilizing same | |
| CN105733962B (en) | A kind of trichoderma aureoviride HLMM-04 bacterial strain and the method for preparing tobacco aromaticss | |
| CN104232492A (en) | Tobacco-derived cellulase-producing microbe strain and application thereof | |
| CN105779307A (en) | Trichoderma aureoviride strain and method for preparing Pu'er Tea tobacco flavor | |
| CN107384838B (en) | Microbial preparation for improving tobacco quality | |
| CN107760618B (en) | Method for improving tobacco leaf quality by using sporulation bacteria | |
| CN115226929B (en) | Seasonal cigar tobacco leaf biological medium fermentation method | |
| CN104762167A (en) | Mechanized production process for multi-strain yellow wine raw wheat koji | |
| CN104911110B (en) | It is a kind of can degraded cellulose function stem and its application in tobacco |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |