CN105566086A - Method for extracting coenzyme Q 10 from tobacco - Google Patents

Method for extracting coenzyme Q 10 from tobacco Download PDF

Info

Publication number
CN105566086A
CN105566086A CN201610107093.7A CN201610107093A CN105566086A CN 105566086 A CN105566086 A CN 105566086A CN 201610107093 A CN201610107093 A CN 201610107093A CN 105566086 A CN105566086 A CN 105566086A
Authority
CN
China
Prior art keywords
salanesol
coenzyme
molecular distillation
solanesol
methyl dimethoxy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610107093.7A
Other languages
Chinese (zh)
Inventor
劳雪红
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi Beibu Gulf Pharmaceutical Co Ltd
Original Assignee
Guangxi Beibu Gulf Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Beibu Gulf Pharmaceutical Co Ltd filed Critical Guangxi Beibu Gulf Pharmaceutical Co Ltd
Priority to CN201610107093.7A priority Critical patent/CN105566086A/en
Publication of CN105566086A publication Critical patent/CN105566086A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C46/00—Preparation of quinones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C29/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom not belonging to a six-membered aromatic ring
    • C07C29/74—Separation; Purification; Use of additives, e.g. for stabilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C29/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom not belonging to a six-membered aromatic ring
    • C07C29/74—Separation; Purification; Use of additives, e.g. for stabilisation
    • C07C29/76—Separation; Purification; Use of additives, e.g. for stabilisation by physical treatment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C29/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom not belonging to a six-membered aromatic ring
    • C07C29/74—Separation; Purification; Use of additives, e.g. for stabilisation
    • C07C29/76—Separation; Purification; Use of additives, e.g. for stabilisation by physical treatment
    • C07C29/80—Separation; Purification; Use of additives, e.g. for stabilisation by physical treatment by distillation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C50/00—Quinones
    • C07C50/26—Quinones containing groups having oxygen atoms singly bound to carbon atoms
    • C07C50/28—Quinones containing groups having oxygen atoms singly bound to carbon atoms with monocyclic quinoid structure

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The invention discloses a method for extracting coenzyme Q 10 from tobacco, which comprises the following steps: (1) pretreatment: adding pure water into tobacco extract extracted from waste inferior tobacco according to same weight, obtaining pasty size by high pressure microjet superfine grinding treatment, then by saponification, crystallizing and filtering, obtaining extract containing solanesol; (2) molecular distillation: purifying the extract containing solanesol in step 1 by molecular distillation to obtain light phase solanesol and heavy phase solanesol; (3) column chromatography separation: dissolving and filtering the heavy phase solanesol in step 2 with acetone, collecting eluant by column chromatography separation, and concentrating to obtain the molecular distilled and purified solanesol; (4) preparation of methyl dimethoxy benzoquinone: performing bromo-etherification and oxidation on methylphenol to obtain the methyl dimethoxy benzoquinone; (5) mixing the light phase solanesol in step 2, the molecular distilled and purified solanesol in step 3 and the methyl dimethoxy benzoquinone in step 4 and reacting to obtain the coenzyme Q 10.

Description

The method of Coenzyme Q10 99.0 is extracted from tobacco leaf
Technical field
The present invention relates to the extracting method of biological technical field, is the method extracting Coenzyme Q10 99.0 from tobacco leaf specifically.
Background technology
Coenzyme Q10 99.0, English name: COENZYMEQ10, CASNo.303-98-0, molecular formula C 59h 90o 4, molecular weight 863.36, be yellow to orange-yellow crystalline powder, odorless, tasteless, is shown in that light easily decomposes, in ethanol and micro dissolution, insoluble in water.It has another name called Ubidecarenone, is a kind of fat-soluble quinone, and its similar is in vitamin K, and the polymerization degree because of the side chain on its parent nucleus six-polyisoamylene base is 10 and gains the name, and is a kind of quinone cyclics.Coenzyme Q10 99.0 is one of indispensable important element of human life, the nutrition of energy human activin cell and cellular energy, have improve body immunity, strengthen anti-oxidant, delay senility and strengthen the functions such as human activity, medically be widely used in cardiovascular system diseases, extensively use it for dietary supplements and foodstuff additive both at home and abroad.
Modern pharmacological research shows, Coenzyme Q10 99.0 mainly contains two effects in vivo: one is be converted in the process of energy at nutritive substance to play an important role in plastosome; Two is have obvious lipoid peroxidization resistant.Coenzyme Q10 99.0 application clinically mainly contains: anti-ageing, protection skin, cardioprotection, antifatigue, strengthen muscle energy, hypertension, treating chronic effort syndromes.
The research application of current state coenzyme Q 10 is main in two: one is for makeup, and Coenzyme Q10 99.0 can remove human free radical because reaching antidotal beautification function; Two is for pharmaceutical industries, and Coenzyme Q10 99.0 can the disease such as adjuvant therapy of cardiovascular.Coenzyme Q10 99.0 is widely used in Japan: calendar year 2001, Coenzyme Q10 99.0 was approved as " protective foods " in the food such as beauty food, antifatigue food and beverage by Japanese health ministry bureau of drug.Japanese health ministry medicine office also announced in 2005, and Coenzyme Q10 99.0 has good nourishing function, and is confirmed by years of researches and application.At present, Japan's every day, nearly 1,200 ten thousand people's long-term takings contained preparation and the healthcare products of Coenzyme Q10 99.0.Chinese food Drug Administration is also used for protective foods in approval Coenzyme Q10 99.0 in 2006, is mainly used in strengthening immunity, anti-oxidant, auxiliary adjustment of blood fat and alleviating physical fatigue.
At present, the method for domestic and international Coenzyme Q10 99.0 raw material production mainly contains: chemical synthesis, microbe fermentation method, biological extraction method etc.(1) chemical synthesis.Coenzyme Q10 99.0 chemical synthesis process is the focus of research both at home and abroad always, nearly half a century, experienced by the research and discovery of countless expert, to start with from two aspects for main point and carry out: first parent nucleus compound introduces Betulaprenol 10 base (decaprenol), another kind method first on parent nucleus compound, introduces shorter side chain, and then the long-chain desired by introducing.This class methods parent nucleus compound and polyisoprene based compound react, and this Coenzyme Q10 99.0 committed step productive rate is all not too high.Therefore, this synthesis strategy is not talkative very desirable.Afterwards, someone utilized CoQ7 synthesizing coenzyme Q 10, because raw material CoQ7 price is very expensive, so this route practical value is little.So they improve synthetic route again, this route is raw materials used cheap and easy to get, and reaction conditions is gentleer, and side chain is combined (90.9%) with parent nucleus compound high yield, and the chain type synthesis that just step is various causes the decline of overall yield.(2) microbe fermentation method.Within 1977, developed country achieves Production by Microorganism Fermentation Coenzyme Q10 99.0, and fermentable extraction method obtained significant progress in recent years.The key problem in technology of microbe fermentation method is throughput and the purification process of the producing strains of Coenzyme Q10 99.0, and wherein extraction method realizes suitability for industrialized production and mainly contains two aspects requirements: the bacterial classification 1. requiring the high quality Coenzyme Q10 99.0 of stable scale production process; 2. the technology of high-purity separation instrument is required.Microbe fermentation method cost is higher, and manufacturing technique requirent is high, and technical difficulty is large.(3) biological extraction method.Being the production method the earliest of Coenzyme Q10 99.0, is also the more method of current domestic use.China carries out the technical study of Coenzyme Q10 99.0 from nineteen seventies, and has built several biological extraction method production lines very soon.Raw material adopts containing the more animal viscera of Q10 or certain plants histoorgan usually, as the animal heart, liver, maize bud etc.Extracting method has saponification method and adsorption chromatography.The Coenzyme Q10 99.0 side chain adopting this method to obtain is the natural product of alltrans structure, and be easily absorbed by the body, product purity is high.Shortcoming is that cost is higher, expensive, is not suitable for industrialization large-scale promotion.
Summary of the invention
The object of this invention is to provide the method extracting Coenzyme Q10 99.0 from tobacco leaf, there is technique simple, be applicable to producing, the advantages such as the time is short.
The present invention is achieved through the following technical solutions, and extracts the method for Coenzyme Q10 99.0, specifically comprise the steps: from tobacco leaf
1) pre-treatment: by inferior tobacco through extracting the tobacco leaf extract obtained, add the pure water of equivalent weight, by the process of high pressure microjet micronizing, obtain paste serous material, then through saponification process, crystallization, filtration, obtaining the medicinal extract containing Salanesol;
2) molecular distillation: by step 1) obtain containing the medicinal extract of Salanesol, by molecular distillation removal of impurities, obtain light phase Salanesol and heavy phase Salanesol;
3) pillar layer separation: by step 2) obtain heavy phase Salanesol acetone solution, filtration, by pillar layer separation, collect elutriant, concentrated, obtain molecular distillation purify after Salanesol;
4) preparation of methyl dimethoxy oxygen base benzoquinones: methylphenol is obtained methyl dimethoxy oxygen base benzoquinones by bromo etherification oxidation;
5) by step 2) the light phase Salanesol, the step 3 that obtain) molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones mixing that obtains, be obtained by reacting Coenzyme Q10 99.0.
Step 1 of the present invention) described in extraction, saponification, crystallization, filtration, with reference to prior art, described high pressure microjet micronizing process, preferably with high-speed shearing emulsion machine process 3-5min, rotating speed is 8000-12000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 80-120MPa, and number of processes is 2-3 time; Described saponification preferably adopts the ethanolic soln of NaOH and petroleum ether system dynamic saponification combined Salanesol.
Step 2) described in molecular distillation, preferred vacuum tightness 0.01Pa, distillation temperature 190 DEG C, scraper plate rotating speed 600r/min, mass flow 1.0mL/min.
Step 3) described in pillar layer separation, preferably adopt ZTC-1 macroporous adsorbent resin, flow velocity be 2 seconds 3, utilize deionized water and 20%, 30%, 40%, 50%, 95% ethanol (v/v) carries out gradient elution.
Step 4) described in the preparation of methyl dimethoxy oxygen base benzoquinones, with reference to prior art, preferable temperature 90 DEG C, raw material: polyoxyethylene glycol: KOH according to the mass ratio of 1:0.15:0.85, time 3h, reaction solvent toluene.
Step 5) described in by step 2) the light phase Salanesol, the step 3 that obtain) and the molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones mixing that obtains, the weight ratio of preferred 2-3:60-75:1-2.
The Coenzyme Q10 99.0 that the present invention obtains, be yellow to orange-yellow crystallinity particle, mobility and water dispersible good, moisture :≤0.2%, heavy metal :≤20ppm, ignition residue :≤0.1%, content: >=98% (HPLC), ash content :≤1.0%.
Compared with prior art, advantage of the present invention:
1, when prior art is extracted, generally carry out fragmentation to plant material or extract after pulverizing obtaining medicinal extract, and the present invention is before molecular distillation, the medicinal extract of Salanesol is contained by the process of high pressure microjet micronizing, and the present invention adopts high pressure microjet micronizing to process tobacco leaf extract, through the effect of high pressure micronizing, improve the stripping of activeconstituents, change very multicomponent the Nomenclature Composition and Structure of Complexes in tobacco leaf simultaneously, expose more binding site, improve its adsorptivity, dispersed, be beneficial to the process of subsequent technique, be beneficial to and improve the yield of activeconstituents and the maintenance of color and luster.
2, the present invention adopts molecular distillation to be a kind of special liquid-liquid separation technology, be carry out in high vacuum conditions be continuously separated process, it not only utilizes the relative volatility between component of mixture, also make full use of the difference of molecular weight between component, therefore its separating power increases significantly than conventional distillation and rectifying, and sepn process is without the need to organic solvent, green, safety, environmental protection, and the original biological nature of isolate can be kept.
3, the present invention's application dynamic fluid flow-liquid distribution principle, utilize the immiscible two-phase solvent of relative movement, in the two-phase being in running balance by the sample component with different partition ratio from, it does not use solid phase carrier to fix phase, overcomes the shortcoming such as sample adsorption, loss, pollution, peak type hangover that solid phase carrier brings.
4, the Coenzyme Q10 99.0 content that prior art obtains generally is no more than 98%, and color and luster is dim, and the Coenzyme Q10 99.0 content that the present invention obtains is more than 98%, and has higher look valency, bright color.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1:
From tobacco leaf, extract the method for Coenzyme Q10 99.0, specifically comprise the steps:
1) pre-treatment: by inferior tobacco through extracting the tobacco leaf extract obtained, add the pure water of equivalent weight, by the process of high pressure microjet micronizing, with high-speed shearing emulsion machine process 3min, rotating speed is 12000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 80MPa, number of processes is 3 times, obtain paste serous material, adopt the ethanolic soln of NaOH and petroleum ether system dynamic saponification combined Salanesol again, crystallization, filtration, obtain the medicinal extract containing Salanesol;
2) molecular distillation: by step 1) obtain containing the medicinal extract of Salanesol, by molecular distillation removal of impurities, vacuum tightness 0.01Pa, distillation temperature 190 DEG C, scraper plate rotating speed 600r/min, mass flow 1.0mL/min, obtain light phase Salanesol and heavy phase Salanesol;
3) pillar layer separation: by step 2) obtain heavy phase Salanesol acetone solution, filtration, pass through pillar layer separation, adopt ZTC-1 macroporous adsorbent resin, flow velocity be 2 seconds 3, utilize deionized water and 20%, 30%, 40%, 50%, 95% ethanol (v/v) carries out gradient elution, collect elutriant, concentrated, obtain the Salanesol after molecular distillation purification;
4) preparation of methyl dimethoxy oxygen base benzoquinones: methylphenol is obtained methyl dimethoxy oxygen base benzoquinones by bromo etherification oxidation;
5) by step 2) the light phase Salanesol, the step 3 that obtain) molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones that obtains mixes with the weight ratio of 2:75:1, is obtained by reacting Coenzyme Q10 99.0.
Embodiment 2:
From tobacco leaf, extract the method for Coenzyme Q10 99.0, specifically comprise the steps:
1) pre-treatment: by inferior tobacco through extracting the tobacco leaf extract obtained, add the pure water of equivalent weight, by the process of high pressure microjet micronizing, with high-speed shearing emulsion machine process 5min, rotating speed is 8000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 120MPa, number of processes is 2 times, obtain paste serous material, adopt the ethanolic soln of NaOH and petroleum ether system dynamic saponification combined Salanesol again, crystallization, filtration, obtain the medicinal extract containing Salanesol;
2) molecular distillation: by step 1) obtain containing the medicinal extract of Salanesol, by molecular distillation removal of impurities, vacuum tightness 0.01Pa, distillation temperature 190 DEG C, scraper plate rotating speed 600r/min, mass flow 1.0mL/min, obtain light phase Salanesol and heavy phase Salanesol;
3) pillar layer separation: by step 2) obtain heavy phase Salanesol acetone solution, filtration, pass through pillar layer separation, adopt ZTC-1 macroporous adsorbent resin, flow velocity be 2 seconds 3, utilize deionized water and 20%, 30%, 40%, 50%, 95% ethanol (v/v) carries out gradient elution, collect elutriant, concentrated, obtain the Salanesol after molecular distillation purification;
4) preparation of methyl dimethoxy oxygen base benzoquinones: methylphenol is obtained methyl dimethoxy oxygen base benzoquinones by bromo etherification oxidation;
5) by step 2) the light phase Salanesol, the step 3 that obtain) molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones that obtains mixes with the weight ratio of 3:60:2, is obtained by reacting Coenzyme Q10 99.0.
Embodiment 3:
From tobacco leaf, extract the method for Coenzyme Q10 99.0, specifically comprise the steps:
1) pre-treatment: by inferior tobacco through extracting the tobacco leaf extract obtained, add the pure water of equivalent weight, by the process of high pressure microjet micronizing, with high-speed shearing emulsion machine process 4min, rotating speed is 10000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 100MPa, number of processes is 3 times, obtain paste serous material, adopt the ethanolic soln of NaOH and petroleum ether system dynamic saponification combined Salanesol again, crystallization, filtration, obtain the medicinal extract containing Salanesol;
2) molecular distillation: by step 1) obtain containing the medicinal extract of Salanesol, by molecular distillation removal of impurities, vacuum tightness 0.01Pa, distillation temperature 190 DEG C, scraper plate rotating speed 600r/min, mass flow 1.0mL/min, obtain light phase Salanesol and heavy phase Salanesol;
3) pillar layer separation: by step 2) obtain heavy phase Salanesol acetone solution, filtration, pass through pillar layer separation, adopt ZTC-1 macroporous adsorbent resin, flow velocity be 2 seconds 3, utilize deionized water and 20%, 30%, 40%, 50%, 95% ethanol (v/v) carries out gradient elution, collect elutriant, concentrated, obtain the Salanesol after molecular distillation purification;
4) preparation of methyl dimethoxy oxygen base benzoquinones: methylphenol is obtained methyl dimethoxy oxygen base benzoquinones by bromo etherification oxidation;
5) by step 2) the light phase Salanesol, the step 3 that obtain) molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones that obtains mixes with the weight ratio of 2.5:70:1.5, is obtained by reacting Coenzyme Q10 99.0.
Comparative example 1:
1) pre-treatment: by inferior tobacco through extracting the tobacco leaf extract obtained, adopt ethanolic soln and the petroleum ether system dynamic saponification combined Salanesol of NaOH, crystallization, filtration, obtain the medicinal extract containing Salanesol;
2) molecular distillation: by step 1) obtain containing the medicinal extract of Salanesol, by molecular distillation removal of impurities, vacuum tightness 0.01Pa, distillation temperature 190 DEG C, scraper plate rotating speed 600r/min, mass flow 1.0mL/min, obtain light phase Salanesol and heavy phase Salanesol;
3) pillar layer separation: by step 2) obtain heavy phase Salanesol acetone solution, filtration, pass through pillar layer separation, adopt ZTC-1 macroporous adsorbent resin, flow velocity be 2 seconds 3, utilize deionized water and 20%, 30%, 40%, 50%, 95% ethanol (v/v) carries out gradient elution, collect elutriant, concentrated, obtain the Salanesol after molecular distillation purification;
4) preparation of methyl dimethoxy oxygen base benzoquinones: methylphenol is obtained methyl dimethoxy oxygen base benzoquinones by bromo etherification oxidation;
5) by step 2) the light phase Salanesol, the step 3 that obtain) molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones that obtains mixes with the weight ratio of 2.5:70:1.5, is obtained by reacting Coenzyme Q10 99.0.
Comparative example 2:
1) pre-treatment: inferior tobacco is smashed, add the pure water of equivalent weight, by the process of high pressure microjet micronizing, with high-speed shearing emulsion machine process 4min, rotating speed is 10000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 100MPa, number of processes is 3 times, obtaining paste serous material, through extracting the tobacco leaf extract obtained, then adopting the ethanolic soln of NaOH and petroleum ether system dynamic saponification combined Salanesol, crystallization, filtration, obtain the medicinal extract containing Salanesol;
2) molecular distillation: by step 1) obtain containing the medicinal extract of Salanesol, by molecular distillation removal of impurities, vacuum tightness 0.01Pa, distillation temperature 190 DEG C, scraper plate rotating speed 600r/min, mass flow 1.0mL/min, obtain light phase Salanesol and heavy phase Salanesol;
3) pillar layer separation: by step 2) obtain heavy phase Salanesol acetone solution, filtration, pass through pillar layer separation, adopt ZTC-1 macroporous adsorbent resin, flow velocity be 2 seconds 3, utilize deionized water and 20%, 30%, 40%, 50%, 95% ethanol (v/v) carries out gradient elution, collect elutriant, concentrated, obtain the Salanesol after molecular distillation purification;
4) preparation of methyl dimethoxy oxygen base benzoquinones: methylphenol is obtained methyl dimethoxy oxygen base benzoquinones by bromo etherification oxidation;
5) by step 2) the light phase Salanesol, the step 3 that obtain) molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones that obtains mixes with the weight ratio of 2.5:70:1.5, is obtained by reacting Coenzyme Q10 99.0.
Look valency is tested:
1, main operational steps: accurate weighing embodiment 3 sample 0.00266g, puts in 10ml volumetric flask, dissolves with distilled water, ultrasonic 5min, after sample solution cool to room temperature, with distilled water constant volume to 10ml, getting 4ml testing sample solution transfers in the cuvette of 1cm, to be checked.Use spectrophotometer to detect sample solution, testing conditions is: determined wavelength: 440nm; Temperature: room temperature; With distilled water zeroing, then sample is detected.
2, calculate:
A value detected, A value exceeds 0.2000-0.6000 scope, so need sample solution to take out 1ml, then constant volume is in 10ml volumetric flask, namely dilutes 10 times, then repeats above-mentioned detecting step, A value detected.
Experimental data is substituted into above-mentioned formula to obtain:
E 1 cm 1 % ( 440 nm ) = A * ( 0.1 / 0.00266 ) * 10
Calculate
Result:

Claims (6)

1. from tobacco leaf, extract the method for Coenzyme Q10 99.0, it is characterized in that, comprise the steps:
1) pre-treatment: by inferior tobacco through extracting the tobacco leaf extract obtained, add the pure water of equivalent weight, by the process of high pressure microjet micronizing, obtain paste serous material, then through saponification process, crystallization, filtration, obtaining the medicinal extract containing Salanesol;
2) molecular distillation: by step 1) obtain containing the medicinal extract of Salanesol, by molecular distillation removal of impurities, obtain light phase Salanesol and heavy phase Salanesol;
3) pillar layer separation: by step 2) obtain heavy phase Salanesol acetone solution, filtration, by pillar layer separation, collect elutriant, concentrated, obtain molecular distillation purify after Salanesol;
4) preparation of methyl dimethoxy oxygen base benzoquinones: methylphenol is obtained methyl dimethoxy oxygen base benzoquinones by bromo etherification oxidation;
5) by step 2) the light phase Salanesol, the step 3 that obtain) molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones mixing that obtains, be obtained by reacting Coenzyme Q10 99.0.
2. the method extracting Coenzyme Q10 99.0 from tobacco leaf according to claim 1, it is characterized in that: step 1) described in the process of high pressure microjet micronizing, first with high-speed shearing emulsion machine process 3-5min, rotating speed is 8000-12000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 80-120MPa, and number of processes is 2-3 time.
3. the method extracting Coenzyme Q10 99.0 from tobacco leaf according to claim 1, is characterized in that: step 2) described in molecular distillation, vacuum tightness 0.01Pa, distillation temperature 190 DEG C, scraper plate rotating speed 600r/min, mass flow 1.0mL/min.
4. the method extracting Coenzyme Q10 99.0 from tobacco leaf according to claim 1, it is characterized in that: step 3) described in pillar layer separation, adopt ZTC-1 macroporous adsorbent resin, flow velocity be 2 seconds 3, deionized water and 20%, 30%, 40%, 50%, 95% ethanol (v/v) carries out gradient elution.
5. the method extracting Coenzyme Q10 99.0 from tobacco leaf according to claim 1, it is characterized in that: step 5) described in by step 2) the light phase Salanesol, the step 3 that obtain) and the molecular distillation that obtains purify after Salanesol and step 4) the methyl dimethoxy oxygen base benzoquinones mixing that obtains, according to the weight ratio of 2-3: 60-75: 1-2.
6. the Coenzyme Q10 99.0 that the method according to any one of claim 1-5 obtains.
CN201610107093.7A 2016-02-26 2016-02-26 Method for extracting coenzyme Q 10 from tobacco Pending CN105566086A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610107093.7A CN105566086A (en) 2016-02-26 2016-02-26 Method for extracting coenzyme Q 10 from tobacco

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610107093.7A CN105566086A (en) 2016-02-26 2016-02-26 Method for extracting coenzyme Q 10 from tobacco

Publications (1)

Publication Number Publication Date
CN105566086A true CN105566086A (en) 2016-05-11

Family

ID=55876799

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610107093.7A Pending CN105566086A (en) 2016-02-26 2016-02-26 Method for extracting coenzyme Q 10 from tobacco

Country Status (1)

Country Link
CN (1) CN105566086A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110066265A (en) * 2018-01-22 2019-07-30 湖南中烟工业有限责任公司 Method for extracting coenzyme Q10 and scopoletin from tobacco leaves

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040151711A1 (en) * 2001-04-19 2004-08-05 West Daniel David Synthesis of coenzyme Q10, ubiquinone
CN1724492A (en) * 2005-07-12 2006-01-25 东北林业大学 Method of extracting jianiol from tobacco
CN1951888A (en) * 2005-10-22 2007-04-25 郑亚津 Process for purifying solanesol from tobacco leaf extract

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040151711A1 (en) * 2001-04-19 2004-08-05 West Daniel David Synthesis of coenzyme Q10, ubiquinone
CN1724492A (en) * 2005-07-12 2006-01-25 东北林业大学 Method of extracting jianiol from tobacco
CN1951888A (en) * 2005-10-22 2007-04-25 郑亚津 Process for purifying solanesol from tobacco leaf extract

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SRINIVASA RAO ATLA等: "A NEW METHOD OF SYNTHESIS OF COENZYME Q10 FROM ISOLATED SOLANESOL FROM TOBACCO WASTE", 《INTERNATIONAL JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110066265A (en) * 2018-01-22 2019-07-30 湖南中烟工业有限责任公司 Method for extracting coenzyme Q10 and scopoletin from tobacco leaves
CN110066265B (en) * 2018-01-22 2022-05-13 湖南中烟工业有限责任公司 Method for extracting coenzyme Q10 and scopoletin from tobacco leaves

Similar Documents

Publication Publication Date Title
CN1974527B (en) Process of preparing high purity chlorogenic acid and flavonid with eucommia leaf
CN105037300B (en) A kind of method for extracting fucoxanthin and brown algae polyphenols comprehensive from sargassum
CN102911138B (en) The extracting and purifying method of fucoxanthin in a kind of brown alga
CN103193610A (en) Preparation method for extracting purified curcumin-related compounds from turmeric
CN102133298B (en) Two-aqueous-phase method for extracting total saponin from bittersweet herb
WO2017028397A1 (en) Method for extracting peroxyergosterol from wall-broken ganoderma lucidum spore powder
CN102086186A (en) Method for preparing tea polyphenol rich in methylated catechin
CN104529738A (en) Extraction preparation method of coenzyme Q10
CN104311616B (en) A kind of extraction high purity cortex fraxini and method of fraxin from Cortex Fraxini
CN104529983A (en) Method for extracting eriodictyol from water chestnut peel
CN105566086A (en) Method for extracting coenzyme Q 10 from tobacco
CN102688255A (en) Application of polysaccharides extracted from wild chrysanthemum in preparation of medicine for enhancing body immunity
CN102093374A (en) Method for efficiently extracting camptothecin derivative
CN110078610B (en) Preparation method of 6-gingerol
CN105541592A (en) Method for extracting coenzyme Q10 from tobacco leaves
KR20160136867A (en) Composition for ameliorating oxidative stress comprising extacts from processed Polygoni Multiflori Radix
CN105566085A (en) Process for extracting coenzyme Q10 from tobacco leaves
CN108484428A (en) A kind of amides compound in matrimony vine and amides compound component and preparation method thereof
CN108794299A (en) The method of purified solanesol
CN1316468A (en) Process for separating and preparing lycopene
CN104610214B (en) Method for rapidly preparing six compounds in Stellera chamaejasme L.
CN113773184B (en) Method for extracting curcumin compounds
CN102304028B (en) Method for separating and purifying resveratrol in polygonum cuspidatum
CN104496815A (en) Preparation method of medicinal chlorogenic acid
CN104292091B (en) A kind of extracting method of Capsorubin

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160511