CN105559074B - A kind of tealeaves volatile essential oil is preparing the application in cancer-preventing health product or anticancer drug - Google Patents
A kind of tealeaves volatile essential oil is preparing the application in cancer-preventing health product or anticancer drug Download PDFInfo
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- 239000000341 volatile oil Substances 0.000 title claims abstract description 31
- 239000002246 antineoplastic agent Substances 0.000 title claims abstract description 11
- 229940041181 antineoplastic drug Drugs 0.000 title claims abstract description 11
- 206010028980 Neoplasm Diseases 0.000 title abstract description 15
- 201000011510 cancer Diseases 0.000 title abstract description 15
- 230000036541 health Effects 0.000 title abstract description 12
- 244000269722 Thea sinensis Species 0.000 claims abstract description 48
- 235000013616 tea Nutrition 0.000 claims abstract description 31
- 235000009569 green tea Nutrition 0.000 claims abstract description 19
- 235000006468 Thea sinensis Nutrition 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 10
- 235000020279 black tea Nutrition 0.000 claims abstract description 7
- 238000004821 distillation Methods 0.000 claims abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 235000020334 white tea Nutrition 0.000 claims abstract description 6
- 235000020338 yellow tea Nutrition 0.000 claims abstract description 5
- 229940079593 drug Drugs 0.000 claims abstract description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 8
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 4
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- 102000004960 NAD(P)H dehydrogenase (quinone) Human genes 0.000 abstract description 19
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- FQTLCLSUCSAZDY-UHFFFAOYSA-N (+) E(S) nerolidol Natural products CC(C)=CCCC(C)=CCCC(C)(O)C=C FQTLCLSUCSAZDY-UHFFFAOYSA-N 0.000 description 1
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 description 1
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 235000019224 Camellia sinensis var Qingmao Nutrition 0.000 description 1
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- QRLVDLBMBULFAL-UHFFFAOYSA-N Digitonin Natural products CC1CCC2(OC1)OC3C(O)C4C5CCC6CC(OC7OC(CO)C(OC8OC(CO)C(O)C(OC9OCC(O)C(O)C9OC%10OC(CO)C(O)C(OC%11OC(CO)C(O)C(O)C%11O)C%10O)C8O)C(O)C7O)C(O)CC6(C)C5CCC4(C)C3C2C QRLVDLBMBULFAL-UHFFFAOYSA-N 0.000 description 1
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- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 1
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- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- FQTLCLSUCSAZDY-ATGUSINASA-N Nerolidol Chemical compound CC(C)=CCC\C(C)=C\CC[C@](C)(O)C=C FQTLCLSUCSAZDY-ATGUSINASA-N 0.000 description 1
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- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
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- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
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- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
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- NEHMKBQYUWJMIP-UHFFFAOYSA-N chloromethane Chemical class ClC NEHMKBQYUWJMIP-UHFFFAOYSA-N 0.000 description 1
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- UVYVLBIGDKGWPX-KUAJCENISA-N digitonin Chemical compound O([C@@H]1[C@@H]([C@]2(CC[C@@H]3[C@@]4(C)C[C@@H](O)[C@H](O[C@H]5[C@@H]([C@@H](O)[C@@H](O[C@H]6[C@@H]([C@@H](O[C@H]7[C@@H]([C@@H](O)[C@H](O)CO7)O)[C@H](O)[C@@H](CO)O6)O[C@H]6[C@@H]([C@@H](O[C@H]7[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O7)O)[C@@H](O)[C@@H](CO)O6)O)[C@@H](CO)O5)O)C[C@@H]4CC[C@H]3[C@@H]2[C@@H]1O)C)[C@@H]1C)[C@]11CC[C@@H](C)CO1 UVYVLBIGDKGWPX-KUAJCENISA-N 0.000 description 1
- UVYVLBIGDKGWPX-UHFFFAOYSA-N digitonine Natural products CC1C(C2(CCC3C4(C)CC(O)C(OC5C(C(O)C(OC6C(C(OC7C(C(O)C(O)CO7)O)C(O)C(CO)O6)OC6C(C(OC7C(C(O)C(O)C(CO)O7)O)C(O)C(CO)O6)O)C(CO)O5)O)CC4CCC3C2C2O)C)C2OC11CCC(C)CO1 UVYVLBIGDKGWPX-UHFFFAOYSA-N 0.000 description 1
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- 230000002708 enhancing effect Effects 0.000 description 1
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- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 229940113087 geraniol Drugs 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
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- 239000001963 growth medium Substances 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
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- 238000000338 in vitro Methods 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 235000020344 instant tea Nutrition 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- 229930007744 linalool Natural products 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
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- WASNIKZYIWZQIP-AWEZNQCLSA-N nerolidol Natural products CC(=CCCC(=CCC[C@@H](O)C=C)C)C WASNIKZYIWZQIP-AWEZNQCLSA-N 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000020339 pu-erh tea Nutrition 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
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- 239000011593 sulfur Chemical group 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/007—Pulmonary tract; Aromatherapy
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Pulmonology (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to tea processing field, discloses a kind of tealeaves volatile essential oil and preparing the application in cancer-preventing health product or anticancer drug.The cancer-preventing health product or anticancer drug refer to that prevention and treatment or treatment have the health care product or drug of the cancer of quinone reductase inductive effect.Tealeaves volatile essential oil is to be extracted using tealeaves as raw material by Simultaneous distillation.The tealeaves volatile essential oil of acquisition not only has the smell of pleasant, can releive anxiety, but also there is certain anticancer effect, 6 big teas include that the volatile essential oil concentration extracted of black tea, green tea, green tea, yellow tea, white tea and dark green tea can induce quinone reductase multiplication in the range of 3~30 μ g/ml and play antitumaous effect.Result of study shows that tealeaves volatile essential oil by inducing quinone reductase to play antitumaous effect, is a kind of potential anti-cancer active matter, can prepare cancer-preventing health product and anticancer drug.
Description
Technical field
The invention belongs to tealeaves intensive processing technical field, in particular to a kind of tealeaves volatile essential oil is protected in preparation anti-cancer
Application in strong product or anticancer drug.
Background technique
Chinese tea drinking is with a long history, and Resources of Tea Plant is abundant, this provides advantageous condition for the person of drinking tea.Tea be most by
One of the healthy beverage that people welcome, contains a variety of function such as tea albumen, tea polyphenols, tea polysaccharide, caffeine, tea saponin in tealeaves
Ingredient is imitated, there are the physiological functions such as antitumor, hypoglycemic, reducing blood lipid, blood pressure lowering, anti-oxidant and enhancing immunity of organisms, as
Health food and drug have wide development prospect.
Tea aroma substance classes are abundant, and content is atomic, typically constitutes from the 0.01~0.05% of dry matter of tea content, green
Tea is about 0.005~0.02%, and black tea is about 0.01~0.03%, and oolong tea is about 0.05%.Tea aroma substance is by organic
Chemical classification can be divided into hydrocarbon, alcohols, aldehydes, ketone, acids, esters, lactone, phenols, oxa- class compound,
Sulfur-bearing class compound, containing nitrogen compound etc., wherein based on alcohols, ketone, esters, aldehydes and hydrocarbon.Fragrance contains
Highest oolong tea is measured, fragrance is because of home environment, tea tree breed, fresh leaf, manufacture craft, quality grade, storage method
Respective distinctive odor characteristic is shown Deng different, is affected especially with manufacture craft: attenuation degree less serious case, fragrance component
Middle nerolidol, jasmine lactone, indoles equal size are higher；Attenuation degree severe one, linalool and its oxidation product, geraniol, benzene
The component contents such as methanol are higher.In terms of tea comprehensive processing, Aqua Folium Camelliae sinensis extraction prepares instant tea, concentrated tea juice is processed for tea beverage
It is used widely, the tea polyphenols separated in tealeaves include that catechin therein is widely used in food additives or production health care food
Product, anticancer research even for field of medicaments such as anti-inflammatory drug and is done in part, but volatile essential oil, that is, aroma substance of tealeaves, protects
Health-care function is paid attention to using seldom work in-process.There is patent using hydro carbons and its derivative (it is derivative to be originated from grease) to 6 big tea
The volatile essential oil of class tealeaves has carried out the extraction of selectivity, prepares high fragrance after having patent to utilize correlation down enzymatic treatment tealeaves
Tea extract, there are also patents, and microwave coupled ion liquid to be utilized to carry out the extraction of tea aroma and for analyzing.
Summary of the invention
In order to solve the disadvantage that the prior art and shortcoming, the purpose of the present invention is to provide a kind of tealeaves volatility essences
Oil is preparing the application in cancer-preventing health product or anticancer drug.This method is simple, quickly.
The purpose of the present invention is achieved through the following technical solutions:
A kind of tealeaves volatile essential oil is preparing the application in cancer-preventing health product or anticancer drug.
The cancer-preventing health product or anticancer drug refer to that prevention and treatment or treatment have the cancer of quinone reductase (QR) inductive effect
The health care product or drug of disease.
The tealeaves volatile essential oil is preferably extracted by Simultaneous distillation using tealeaves as raw material.?
It can be prepared using other methods.
The Simultaneous distillation is specifically according to the following steps: tea raw material being ground, according to every gram of tealeaves original
The ratio of material addition 5~20ml water adds water, obtains tea mixture；Using methylene chloride as extractant, using distilling simultaneously
The methods of extraction equipment extracts, and extraction time is 60min~180min；Obtained solution is transferred to nitrogen in sample bottle to blow
It is dry, obtain tealeaves essential oil.
Described to be extracted specifically according to the following steps using SDE method: tea mixture is placed in round bottom burning
In bottle, it is connected to one end of distilling and extracting device, and heated with electric jacket, kept slightly boiled；The other end of distilling and extracting device connects
Tipping has the round-bottomed flask of methylene chloride, is heated with water-bath, temperature is maintained at 55 DEG C.
When tealeaves dosage≤50 gram, the dosage of the methylene chloride is 50mL；When 50 grams of tealeaves dosage >, described two
The dosage of chloromethanes is ml of tealeaves grams corresponding number.
The tealeaves is black tea, green tea, green tea, yellow tea, white tea and dark green tea.
The present invention induces its quinone reductase to by representative of murine hepatocarcinoma cell Hepa1c1c7 using tealeaves volatile essential oil
(QR) activity rises to anticancer activity evaluation, and tealeaves volatile essential oil there is preferable inhibition to make Hepa1c1c7 cancer cell line
With being a kind of potential cancer-resisting active material.
The present invention have the following advantages compared with prior art and the utility model has the advantages that
(1) present invention, which extracts obtained tealeaves volatile essential oil, has stronger cancer-preventing health and anticancer effect, and this hair
Operation is simple for Simultaneous distillation involved in bright and correlation method, at low cost, and the rate of recovery of essential oil is high；Therefore, have
Higher practical application and promotional value.
(2) the obtained tealeaves essential oil of the present invention can be applied in aromatotherapy, can not only relieving mood, eliminate burnt
Consider, and also there is the health-care efficacy for inhibiting growth of cancer cells, have a good application prospect, can be applied to medicine, health care
Product and daily use chemicals industry.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
The preparation method of 1 tealeaves volatile essential oil of embodiment
(1) it weighs 25g and grinds tea sample in 1L round-bottomed flask, according to the ratio of every gram of tea raw material addition 5~20ml water
Level-one water is added, is connected to one end of SDE method, and heated with electric jacket, is kept slightly boiled；Other end connection dress
There is the round-bottomed flask of 50mL methylene chloride, heated with water-bath, temperature is maintained at 55 DEG C；Extraction time is 60~180min.
(2) it collects distillate after extracting to be transferred in sample bottle, nitrogen, which is blown, volatilizes methylene chloride, obtains tealeaves volatility essence
Oil samples.
The research of 2 tealeaves volatile essential oil antitumor cell efficacy in vitro of embodiment, comprising the following steps:
(1) Hepa1c1c7 murine hepatocarcinoma cell suspension, logarithmic growth phase, cell in good condition, with 0.25% are prepared
Trypsase (mass percent) digestion, is configured to 5 × 10 with culture medium3The cell suspension of a cell/mL is inoculated in the training of 96 holes
It supports in plate, every 200 μ L of hole, is inoculated with two boards.37 DEG C, 5% (percentage by volume) CO2Cell is cultivated for 24 hours in incubator.
(2) tealeaves volatile essential oil is used to the a-MEM culture solution (volume basis for containing 10% fetal calf serum according to doses
Number) it is configured to certain concentration solution with sample, 150 μ L solution are added in every hole, will be added without murine hepatocarcinoma cell group as sky
White control group, control group add equivalent fresh medium.37 DEG C, 5%CO2Continue to cultivate cell in incubator (percentage by volume).
(3) after culture for 24 hours, two boards are divided into A plate and B plate.
The measurement of cell survival rate
The crystal violet of A plate 0.2% (mass percent) of addition, every 100 μ L of hole, (20-25 DEG C) placement 10-15min of room temperature,
Paper suck dry moisture is used after rinsing in water.0.5%SDS (mass percent) solution, the 100rpm on shaking table, 37 DEG C of oscillations are added
After 1h, absorbance value (OD value) is surveyed at 610nm with microplate reader.
The measurement of quinone reductase (QR)
The digitonin (mass percent) of 50 μ L 0.08% is added in B plate, on 100rpm shaking table, 37 DEG C of oscillations >
0.5h.Then by reaction system: being poured into loading slot after enzyme solution, MTT, menadione mixing, it is anti-to be rapidly added 200 μ L/well enzymes
Answer liquid.Using microplate reader using KINETIC METHOD in 490nm, 1min measurement is primary.
(4) calculation method
The opposite QR activity of sample indicates with CD value, and being defined as opposite QR activity, to reach corresponding sample when multiplication dense
Degree, when cell viability is greater than 0.5, and opposite QR activity is greater than 2, it is believed that there is sample the activity of induction quinone reductase to have
Anticancer activity.
The anticancer activity of 3 tealeaves essential oil of embodiment evaluates (one)
Black tea, green tea, dark green tea, white tea, green tea, the yellow tea method that totally 6 big teas representatives use embodiment 1 are obtained into tealeaves
Volatile essential oil obtains the tool that induction quinone reductase generates multiplication by linear fit curve using method as described in example 2
Bulk concentration.
The big teas tealeaves essential oil of table 1. 6 induces quinone reductase to generate the concentration (μ g/ml) to double
| Teas | Teas is tested to represent | QR multiplication concentration |
| Black tea | Big-leaf species in yunnan black tea | 5.32 |
| Green tea | Big-leaf species in yunnan green tea | 12.32 |
| Dark green tea | Big-leaf species in yunnan Pu'er tea | 3.03 |
| White tea | Fuding white tea | 8.96 |
| Green tea | Phoenix is single from (honey is blue fragrant) | 23.08 |
| Yellow tea | Hunan Junshan Silver Needle Tea | 7.39 |
Result above is the average value of parallel laboratory test three times
According to table 1 the result shows that: the tea of different teas volatilization oil concentration can induce quinone also in the range of 3~30 μ g/mL
Antitumaous effect is played in protoenzyme multiplication.Illustrate that tealeaves volatile essential oil is a kind of potential cancer-resisting active material.
The anticancer activity of 4 tealeaves essential oil of embodiment evaluates (two)
Different flavor Various Seasonal oolong tea (green tea) is obtained into tealeaves volatile essential oil using the method for embodiment 1, is made
With method as described in example 2, the specific concentration that induction quinone reductase generates multiplication is obtained by linear fit curve.
2. different flavor Various Seasonal phoenix Dan Congcha (green tea) of table etc. induces QR to generate the concentration (μ g/mL) to double
Result above is the average value of parallel laboratory test three times
According to table 2 the result shows that: the phoenix of different flavor Various Seasonal it is single from oolong tea volatilization oil concentration in 3~30 μ g/
It equally can induce quinone reductase multiplication in the range of mL and play antitumaous effect.Illustrate that tealeaves volatile essential oil is a kind of potential
Cancer-resisting active material.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (4)
1. a kind of tealeaves volatile essential oil is preparing the application in anticancer drug；The anticancer drug refers to that treatment has quinone reduction
The drug of the liver cancer of enzyme induction effect；The tealeaves volatile essential oil is to be mentioned using tealeaves as raw material by Simultaneous distillation
It obtains；The Simultaneous distillation is specifically according to the following steps: tea raw material being ground, according to every gram of tealeaves original
The ratio of material addition 5~20ml water adds water, obtains tea mixture；Using methylene chloride as extractant, using distilling simultaneously
Extraction equipment method extracts, and extraction time is 60min~180min；Obtained solution is transferred to nitrogen in sample bottle to dry up
It is dry, obtain tealeaves essential oil.
2. application according to claim 1, it is characterised in that: described to be extracted specifically using SDE method
According to the following steps: tea mixture sets the one end for being connected to distilling and extracting device in a round bottom flask, and is added with electric jacket
Heat is kept slightly boiled；The other end connection of distilling and extracting device is equipped with the round-bottomed flask of methylene chloride, is heated with water-bath, temperature
It is maintained at 55 DEG C.
3. application according to claim 1, it is characterised in that: when tealeaves dosage≤50 gram, the use of the methylene chloride
Amount is 50mL；When 50 grams of tealeaves dosage >, the dosage of the methylene chloride is ml of tealeaves grams corresponding number.
4. application according to claim 1, it is characterised in that: the tealeaves be black tea, green tea, green tea, yellow tea, white tea or
Dark green tea.
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