CN105557684A - Plasticizing method of Japanese red maple leaves - Google Patents
Plasticizing method of Japanese red maple leaves Download PDFInfo
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- CN105557684A CN105557684A CN201511026231.0A CN201511026231A CN105557684A CN 105557684 A CN105557684 A CN 105557684A CN 201511026231 A CN201511026231 A CN 201511026231A CN 105557684 A CN105557684 A CN 105557684A
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- red maple
- japanese red
- peg
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- plasticizer solution
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Abstract
The invention discloses a plasticizing method of Japanese red maple leaves. The method comprises the following steps: placing color protected and press dried Japanese red maple leaves in a plasticizer solution with the PEG volume concentration of 20% for 5-7h, taking out the Japanese red maple leaves, immersing the Japanese red maple leaves in a next stage plasticizer solution for 5-7h, and repeating above steps until a whole 3-6 stage dehydration process is completed, wherein PEG volume concentration of the next stage plasticizer solution is 15-25% higher than that of a last stage plasticizer solution, and the PEG volume concentration of the final stage plasticizer solution is 100%. The method uses a PEG mixed liquid to penetrate and plasticize Japanese red maple leaf specimen plants, so the plasticizing time is shortened, and the original ecology image and the fresh and alive feeling of the Japanese red maple leaves are kept. Japanese red maple leaf specimens produced in the invention have the advantages of unlikely fading and crushing, unlikely worm eating and mildewing, and facilitation of long-term preservation.
Description
Technical field
The present invention relates to a kind of preparation method of plant leaf blade specimen, be specifically related to a kind of plasticizing method of Japanese red maple blade.
Background technology
The leaf grace of Japanese red maple, summer is bright-coloured green, has good ornamental value.Traditional blades preserving type is mostly pressed dry and is preserved, and can not keep ecosystem shape after causing dehydration, does not have fresh and alive sense.By plasticizing, on the maintenance non-fading basis of blade, slowly displace leaf water, ensure that leaf morphology is complete, bright in colour.With regard to current preparation method, there is certain defect, mainly plant leaf blade specimen fugitive color, fragmentation and easily damaged by worms, gone mouldy, be unfavorable for long-term preservation.
Summary of the invention
In view of this, the application provides a kind of plasticizing method of Japanese red maple blade, and the Japanese red maple leaf blade specimen utilizing the method to make not fugitive color, fragmentation, is not easily damaged by worms, goes mouldy, be beneficial to long-term preservation.
For solving above technical problem, technical scheme provided by the invention is a kind of plasticizing method of Japanese red maple blade, comprises the following steps:
A, by the disinfection of Japanese red maple blade, then clean with distilled water flushing, then soak in distilled water, rinse;
B, condition to the Japanese red maple leaf sheet that the Japanese red maple blade after rinsing is placed in 85-90 DEG C first become after yellowish-brown becomes green again and takes out, clean, press dry;
C, the Japanese red maple blade obtained after pressing dry is placed in plasticizer solution soaks, described plasticizer solution displacement is till the water content <1% of described Japanese red maple blade; Wherein, described plasticizer solution displacement is replaced step by step according to PEG volumetric concentration in described plasticizer solution.
Further, described steps A concentration is the ethanolic solution disinfection of 65%-75%.
Further, described steps A concentration is the ethanolic solution disinfection of 70%.
Further, described steps A is by Japanese red maple blade ethanolic solution sterilization 4-6min.
Further, the Japanese red maple blade after sterilization is soaked 10-20min by described steps A in distilled water.
Further, plasticizer solution described in described step C is the mixed liquor of isopropyl alcohol, PEG and water and glycerine.
Further, in plasticizer solution described in described step C, the concentration of glycerine is 1%.
Further, the process that described in described step C, plasticizer solution is replaced step by step is specially: the Japanese red maple blade obtained after pressing dry is placed in PEG volumetric concentration be 20% plasticizer solution soak 5-7 hours, then take out and to be dipped in next stage plasticizer solution 5-7 hours, repeat this operation, the process need of whole dehydration 3-6 grades; In next stage plasticizer solution, the volumetric concentration of PEG increases progressively 15%-25% than PEG volumetric concentration in upper level plasticizer solution, and the volumetric concentration of the plasticizer solution PEG of afterbody is 100%, and Japanese red maple blade completes replacement process.
Further, the process that described in described step C, plasticizer solution is replaced step by step is specially: the Japanese red maple blade obtained after pressing dry is placed in PEG volumetric concentration be 20% plasticizer solution soak 6 hours, then take out and to be dipped in next stage plasticizer solution 6 hours, repeat this operation, the process need of whole dehydration 3-6 grades; In next stage plasticizer solution, the volumetric concentration of PEG increases progressively 20% than PEG volumetric concentration in upper level plasticizer solution, and the volumetric concentration of the plasticizer solution PEG of afterbody is 100%, and Japanese red maple blade completes replacement process.
The technical scheme of second aspect provided by the invention is the plasticiser for the plasticizing of Japanese red maple blade, by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 15%-25%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, the gradient that concentration increases successively is carried out to sample and soaks.
Further, for the plasticiser of Japanese red maple blade plasticizing, by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 20%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, the gradient that concentration increases successively is carried out to sample and soaks.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention increases concentration step by step PEG solution replacement method under adopting normal temperature is dewatered, and the replacement operator from low concentration to high concentration is simple, convenient.After dehydration, the crimp contraction of sample is down in 1%, and sample general shape, without change, is conducive to the observation of later stage sample.
Blade after pressing dry is immersed in plasticiser and plastifies by the present invention, plasticiser adopts isopropyl alcohol and water the moisture in plasticiser and blade can be made at short notice to complete displacement as cosolvent, the particularly displacement of deep structure, effectively shortens the time of preparation of specimen; Increase the PEG solution replacement of concentration under adopting normal temperature step by step, be conducive to the interior displacement fully of muscle of parenchymal viscera and deep layer.
Method of the present invention makes leaf blade specimen can keep ecosystem shape, has fresh and alive sense.By plasticizing, on the maintenance non-fading basis of blade, slowly displace leaf water, ensure that leaf morphology is complete, bright in colour, not fugitive color, fragmentation, damaged by worms, go mouldy, be beneficial to long-term preservation.
Embodiment
In order to make those skilled in the art understand technical scheme of the present invention better, below in conjunction with specific embodiment, the present invention is described in further detail.
The plasticizing method of Japanese red maple blade of the present invention, comprises the following steps:
A, be 65%-75% ethanolic solution disinfection 4-6min by fresh Japanese red maple blade concentration, then clean with distilled water flushing, then soak in 10-20min in distilled water, rinse;
B, condition to the Japanese red maple leaf sheet that the Japanese red maple blade after rinsing is placed in 85-90 DEG C first become after yellowish-brown becomes green again and takes out, clean, press dry;
C, the Japanese red maple blade obtained after pressing dry is placed in plasticizer solution soaks, described plasticizer solution displacement is till the water content <1% of described Japanese red maple blade; Wherein,
Described plasticizer solution is the mixed liquor of isopropyl alcohol, PEG and water and glycerine, and the concentration of glycerine in this mixed liquor is 1%.Described plasticizer solution displacement is replaced step by step according to PEG volumetric concentration in described plasticizer solution.
The process that described plasticizer solution is replaced step by step is specially: the Japanese red maple blade obtained after pressing dry is placed in PEG volumetric concentration be 20% plasticizer solution soak 5-7 hours, then take out and to be dipped in next stage plasticizer solution 5-7 hours, repeat this operation, the process need of whole dehydration 3-6 grades; In next stage plasticizer solution, the volumetric concentration of PEG increases progressively 15%-25% than PEG volumetric concentration in upper level plasticizer solution, and the volumetric concentration of the plasticizer solution PEG of afterbody is 100%, and Japanese red maple blade completes replacement process.
Preferably, the process that described plasticizer solution is replaced step by step is specially: the Japanese red maple blade obtained after pressing dry is placed in PEG volumetric concentration be 20% plasticizer solution soak 6 hours, then take out and to be dipped in next stage plasticizer solution 6 hours, repeat this operation, the process need of whole dehydration 3-6 grades; In next stage plasticizer solution, the volumetric concentration of PEG increases progressively 20% than PEG volumetric concentration in upper level plasticizer solution, and the volumetric concentration of the plasticizer solution PEG of afterbody is 100%, and Japanese red maple blade completes replacement process.
In concrete enforcement, the present invention is to plastify Japanese red maple blade as an application of the present invention.
Control group-existing plasticizing method plastifies Japanese red maple blade
Be 65%-75% ethanolic solution disinfection 4-6min by fresh Japanese red maple blade concentration, then clean with distilled water flushing, then soak in 10-20min in distilled water, rinse; Condition to the Japanese red maple leaf sheet that Japanese red maple blade after rinsing is placed in 85-90 DEG C is first become after yellowish-brown becomes green again and takes out, clean, press dry; Japanese red maple blade after pressing dry is adopted the mixed liquor of ethanol and glycerine or 5%-100% plastifying liquid (PEG) in carry out gradient immersion, it is 15%-30% that the volume fraction of each gradient PEG increases, soak time is respectively and plastifies for 7-20 hours, take out blade after plasticizing to press dry, obtain Japanese red maple blade plasticizing sample.
Experimental group-plasticizing method of the present invention plastifies Japanese red maple blade
Be 65%-75% ethanolic solution disinfection 4-6min by fresh Japanese red maple blade concentration, then clean with distilled water flushing, then soak in 10-20min in distilled water, rinse; Condition to the Japanese red maple leaf sheet that Japanese red maple blade after rinsing is placed in 85-90 DEG C is first become after yellowish-brown becomes green again and takes out, clean, press dry; The plastifying liquid (PEG) that Japanese red maple blade after pressing dry puts into 20%-100% is carried out gradient immersion, and it is 15%-25% that the volume fraction of each gradient PEG increases, and soak time is respectively 5-7 hours.Soak rear taking-up, with rinsing out remaining solution in clear water.Sample after plasticizing is pressed dry, obtains Japanese red maple blade plasticizing sample.
Embodiment 1-Japanese red maple blade fusion time check experiment
To plastify Japanese red maple blade, respectively above-described embodiment is tested, the Japanese red maple leaf blade specimen obtained, same mode is all adopted to manage under same condition, plastify under same temperature and humidity, measure the fusion time of gained sample, the data obtained list 1 is as follows:
Table 1-Japanese red maple blade fusion time control experiment
| Experimental subjects | Control group 1 | Experimental group 1 |
| Fusion time | 60-100 hours | 25-42 hours |
Embodiment 2-Japanese red maple leaf blade specimen holding time check experiment
To plastify Japanese red maple blade, respectively above-described embodiment is tested, the Japanese red maple leaf blade specimen obtained, all adopt same mode to manage under same condition, preserve under same temperature and humidity, gained sample is positioned over temperature 20 ~ 25 DEG C, the indoor preservation of humidity 50% ~ 60%, observing time is 2 years, and measure the preservation primary color time of gained sample, the data obtained list 2 is as follows:
Table 2-Japanese red maple leaf blade specimen preserves primary color time control experiment
| Experimental subjects | Control group 1 | Experimental group 1 |
| Preserve primary color time | Below half a year | More than 1 year |
Embodiment 3-Japanese red maple leaf blade specimen light control is tested
To plastify Japanese red maple blade, respectively above-described embodiment is tested, the Japanese red maple leaf blade specimen obtained, same mode is all adopted to manage under same condition, gained sample being positioned over illumination is strong illumination 10 hours under the condition of 5000lx ± 500lx, observed every 10 days respectively, within 60 days, record the primary colors situation of gained sample afterwards, the data obtained list 3 is as follows:
Table 3-Japanese red maple leaf blade specimen primary colors situation control experiment
| Experimental subjects | Control group 1 | Experimental group 1 |
| Primary colors situation | Fade | Colour-fast |
The specific embodiment of the present invention is as follows:
Embodiment 1: described plasticizer solution is by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 15%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, carry out to sample the gradient that concentration increases successively and soak, each soak time is 5 hours.
Embodiment 2: described plasticizer solution is by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 25%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, carry out to sample the gradient that concentration increases successively and soak, each soak time is 5 hours.
Embodiment 3: described plasticizer solution is by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 15%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, carry out to sample the gradient that concentration increases successively and soak, each soak time is 6 hours.
Embodiment 4: described plasticizer solution is by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 25%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, carry out to sample the gradient that concentration increases successively and soak, each soak time is 6 hours.
Embodiment 5: described plasticizer solution is by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 20%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, carry out to sample the gradient that concentration increases successively and soak, each soak time is 5 hours.
Embodiment 6: described plasticizer solution is by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 20%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, carry out to sample the gradient that concentration increases successively and soak, each soak time is 6 hours.
Same to plastify Japanese red maple blade, adopt above-mentioned embodiment 1-6 to plastify.Adopt aforementioned same mode to go to measure the fusion time of gained sample, primary color preservation time, primary colors situation, the data obtained list 4 is as follows:
Table 4-specific embodiment of the invention
| Embodiment | 1 | 2 | 3 | 4 | 5 | 6 |
| Fusion time (hour) | 35 | 25 | 42 | 30 | 25 | 30 |
| The primary color preservation time (year) | 1.2 | 1.2 | 1.2 | 1.2 | 1.8 | 2 |
| Primary colors situation (variable color) | Green | Slightly fade | Green | Slightly fade | Green | Bud green |
As can be seen from table 1, table 2, table 3: in control group, Japanese red maple blade fusion time is 60-100 hours, the primary color preservation time is half a year below, fugitive color; In experimental group, Japanese red maple blade fusion time is 25-42 hours, and the primary color preservation time is more than 1 year, not fugitive color; Known experimental group substantially reduces the fusion time making Japanese red maple leaf blade specimen, and improves the primary color preservation time, and not fugitive color, improves the holding time.
As can be seen from Table 4: adopt embodiment 6 i.e. best mode for carrying out the invention to be applied to and make Japanese red maple leaf blade specimen, substantially reduce fusion time, and improve the holding time.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred embodiment should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a plasticizing method for Japanese red maple blade, is characterized in that, comprises the following steps:
A, by the disinfection of Japanese red maple blade, then clean with distilled water flushing, then soak in distilled water, rinse;
B, condition to the Japanese red maple leaf sheet that the Japanese red maple blade after rinsing is placed in 85-90 DEG C first become after yellowish-brown becomes green again and takes out, clean, press dry;
C, the Japanese red maple blade obtained after pressing dry is placed in plasticizer solution soaks, described plasticizer solution displacement is till the water content <1% of described Japanese red maple blade; Wherein, described plasticizer solution displacement is replaced step by step according to PEG volumetric concentration in described plasticizer solution.
2. the plasticizing method of Japanese red maple blade according to claim 1, is characterized in that, described steps A concentration is the ethanolic solution disinfection of 65%-75%.
3. the plasticizing method of Japanese red maple blade according to claim 2, is characterized in that, described steps A concentration is the ethanolic solution disinfection of 70%.
4. the plasticizing method of Japanese red maple blade according to claim 1, is characterized in that, described steps A is by Japanese red maple blade ethanolic solution sterilization 4-6min.
5. the plasticizing method of Japanese red maple blade according to claim 1, is characterized in that, the Japanese red maple blade after sterilization is soaked 10-20min by described steps A in distilled water.
6. the plasticizing method of Japanese red maple blade according to claim 1, is characterized in that, plasticizer solution described in described step C is the mixed liquor of isopropyl alcohol, PEG and water and glycerine.
7. the plasticizing method of Japanese red maple blade according to claim 6, is characterized in that, in plasticizer solution described in described step C, the concentration of glycerine is 1%.
8. the plasticizing method of Japanese red maple blade according to claim 1, it is characterized in that, the process that described in described step C, plasticizer solution is replaced step by step is specially: the Japanese red maple blade obtained after pressing dry is placed in PEG volumetric concentration be 20% plasticizer solution soak 5-7 hours, then take out and to be dipped in next stage plasticizer solution 5-7 hours, repeat this operation, the process need of whole dehydration 3-6 grades; In next stage plasticizer solution, the volumetric concentration of PEG increases progressively 15%-25% than PEG volumetric concentration in upper level plasticizer solution, and the volumetric concentration of the plasticizer solution PEG of afterbody is 100%, and Japanese red maple blade completes replacement process.
9. the plasticizing method of Japanese red maple blade according to claim 8, it is characterized in that, the process that described in described step C, plasticizer solution is replaced step by step is specially: the Japanese red maple blade obtained after pressing dry is placed in PEG volumetric concentration be 20% plasticizer solution soak 6 hours, then take out and to be dipped in next stage plasticizer solution 6 hours, repeat this operation, the process need of whole dehydration 3-6 grades; In next stage plasticizer solution, the volumetric concentration of PEG increases progressively 20% than PEG volumetric concentration in upper level plasticizer solution, and the volumetric concentration of the plasticizer solution PEG of afterbody is 100%, and Japanese red maple blade completes replacement process.
10. for the plasticiser of Japanese red maple blade plasticizing, it is characterized in that, by the PEG solution composition of at least 5 different volumes marks, and in the solution of adjacent two concentration the difference of PEG volume fraction 15%-25%, in the solution of least concentration, the volume fraction of PEG is 20%, and in the solution of maximum concentration, the volume fraction of PEG is 100%; During use, the gradient that concentration increases successively is carried out to sample and soaks.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108189613A (en) * | 2018-03-08 | 2018-06-22 | 河南科技大学 | A kind of immortality flower and preparation method thereof |
| CN108849862A (en) * | 2018-06-19 | 2018-11-23 | 郑州百草文化传播有限公司 | A kind of plant plasticizing Slide processing |
| CN109551954A (en) * | 2018-12-13 | 2019-04-02 | 重庆加倍健电子商务有限公司 | A kind of woodwork inlays the process of resin package dried flower |
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| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
| CN103548817A (en) * | 2013-11-18 | 2014-02-05 | 重庆市中药研究院 | Preparation method of plant plasticized specimen |
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2015
- 2015-12-30 CN CN201511026231.0A patent/CN105557684A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
| CN103548817A (en) * | 2013-11-18 | 2014-02-05 | 重庆市中药研究院 | Preparation method of plant plasticized specimen |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108189613A (en) * | 2018-03-08 | 2018-06-22 | 河南科技大学 | A kind of immortality flower and preparation method thereof |
| CN108849862A (en) * | 2018-06-19 | 2018-11-23 | 郑州百草文化传播有限公司 | A kind of plant plasticizing Slide processing |
| CN109551954A (en) * | 2018-12-13 | 2019-04-02 | 重庆加倍健电子商务有限公司 | A kind of woodwork inlays the process of resin package dried flower |
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