CN105557614A - Roe embryo heartbeat observation device - Google Patents
Roe embryo heartbeat observation device Download PDFInfo
- Publication number
- CN105557614A CN105557614A CN201610019079.1A CN201610019079A CN105557614A CN 105557614 A CN105557614 A CN 105557614A CN 201610019079 A CN201610019079 A CN 201610019079A CN 105557614 A CN105557614 A CN 105557614A
- Authority
- CN
- China
- Prior art keywords
- fish
- chamber
- egg embryo
- observation device
- water hole
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a roe embryo heartbeat observation device which consists of an accommodating unit and a constant-temperature water flowing unit, wherein the constant-temperature water flowing unit is communicated with the accommodating unit; the accommodating unit comprises an upper cover plate, a lower bottom plate and side plates; the upper cover plate and the lower bottom plate are both permeable to light; at least one chamber is further formed in the accommodating unit; water exchange holes are formed in each of the chambers; gaps are reserved between the chambers and the side plates of the accommodating unit and among the chambers; the chambers are filled with light-transmitting fiber agglomerates; a flocculent fiber layer is laid at the bottom of each of the chambers. The invention aims to provide a stable observation environment and good observation conditions for roe embryo heartbeat observation tests, and further improve the observation result accuracy.
Description
Technical field
The invention belongs to biomedical and Environmental Toxicological application, be specifically related to a kind of fish-egg embryo heartbeat observation device.
Background technology
Fish and fish-egg embryo very responsive to harmful substance, become biomedical and the mode standard biology of ecotoxicology, conventional toxicity test, molecule and the many aspects such as cell ecological toxicological study and biotic induce effect detection are widely used in, by the situation of observation fish-egg embryo heartbeat under toxicant contamination condition, judge the poisonous effect of fish-egg embryonic development, thus evaluation analysis toxicant.
In above-mentioned test, in order to obtain fish-egg embryo heartbeat observed result more accurately, the factors such as the environmental temperature of control observation device, illumination are needed to ensure the normal development of fish-egg embryo, and prevent environmental contaminants from entering culture fluid, thus ensure unitary variant test, need to ensure that embryo is fixing firm simultaneously, be beneficial to observation.
At present, fish-egg embryo heartbeat is observed, and generally selects 24 orifice plates as contamination container, is placed on inverted microscope and carries out the observation of fish-egg embryo heartbeat.But the 24 orifice plates observed pattern be directly placed on inverted microscope makes fish-egg embryo and test culture fluid directly be exposed in room air, is difficult to the observation condition that guarantee one is good, have impact on the accuracy of test.
Summary of the invention
The present invention proposes for solving the problem, and object is by providing a kind of fish-egg embryo heartbeat observation device, improves the accuracy of fish-egg embryo heartbeat observation further.
For achieving the above object, present invention employs following technical scheme:
A kind of fish-egg embryo heartbeat observation device, for placing the fish-egg embryo needing observation, is characterized in that, comprise: accomodating unit, for holding fish-egg embryo, comprise the upper cover plate of printing opacity, lower shoe, and the side plate be connected between upper cover plate, lower shoe and with lower shoe; Thermostatted water flow unit, comprises inlet opening, apopore, and inlet opening, apopore are arranged on accomodating unit, and are communicated with side plate; Wherein, be provided with at least one chamber in accomodating unit, chamber is provided with exchanging water hole, between chamber and side plate, between chamber and chamber, all leave space.
Fish-egg embryo heartbeat observation device provided by the invention, can also have such feature, wherein, exchanging water hole, comprise at least one pair of be staggered high-order exchanging water hole and low level exchanging water hole of arranging, high-order exchanging water hole is arranged at the top of chamber, and low level exchanging water hole is arranged at the bottom of chamber.
Fish-egg embryo heartbeat observation device provided by the invention, can also have such feature, wherein, and the diameter 2-4mm of exchanging water hole.
Fish-egg embryo heartbeat observation device provided by the invention, can also have such feature, wherein, be filled with coma in chamber, for cushioning the impact that water body flow causes chamber.
Fish-egg embryo heartbeat observation device provided by the invention, such feature can also be had, wherein, coma is bonding with upper cover plate, the position that upper cover plate is connected with coma, have not erasable mark, the shape of mark is consistent along shape with on chamber inner wall, the size of the shape of mark be less than chamber inner wall on along size.
Fish-egg embryo heartbeat observation device provided by the invention, can also have such feature, and wherein, coma is printing opacity group.
Fish-egg embryo heartbeat observation device provided by the invention, can also have such feature, and wherein, the bottom lay of chamber has fiber batt layer, for fixing fish-egg embryo.
Fish-egg embryo heartbeat observation device provided by the invention, can also have such feature, and wherein, inlet opening and apopore are crisscross arranged on accomodating unit.
Fish-egg embryo heartbeat observation device provided by the invention, can also have such feature, wherein, the profile of accomodating unit is square, in circular, ellipse any one.
Fish-egg embryo heartbeat observation device provided by the invention, can also have such feature, wherein, chamber shape be cylinder, internal diameter 2cm, degree of depth 2cm, the quantity of chamber is 4-16.
Invention effect and effect
According to fish-egg embryo heartbeat observation device provided by the invention, be made up of accomodating unit, thermostatted water flow unit, thermostatted water flow unit is communicated with accomodating unit.Accomodating unit comprises upper cover plate, lower shoe, side plate, upper cover plate, lower shoe are printing opacity, side plate is between upper cover plate, lower shoe, and be connected with lower shoe, also be provided with at least one chamber in accomodating unit, chamber is provided with exchanging water hole, between chamber and accomodating unit side plate, between chamber and chamber, all leave space, be filled with light-transmitting fiber group in chamber, cavity bottom lay has fiber batt layer.
Fish-egg embryo heartbeat observation device provided by the invention, the cover plate of the exchanging water hole in the thermostatted water flow unit of thermostatted water, space, on chamber, printing opacity and base plate are provided, coma is place the aerobic environment that fish-egg embryo provides a temperature stabilization, airtight printing opacity, avoid environmental contaminants and enter pollution fish-egg embryo, ensure that under enough viewing ray condition prerequisites, ensure that fish-egg embryo heartbeat observing environment, ensure that the relative unicity of observation experiment condition; Cavity bottom lay down fibre batt layer fixes fish-egg embryo, and in chamber, fiberfill fibers group to cushion in water body flow the impact that chamber causes, and prevents the shake of fish-egg embryo in observation, improves the accuracy of observed result.
Accompanying drawing explanation
Fig. 1 is the upward view of fish-egg embryo heartbeat observation device of the present invention;
Fig. 2 is the front view of fish-egg embryo heartbeat observation device of the present invention;
Fig. 3 is the left view of fish-egg embryo heartbeat observation device of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described.
Fig. 1 is the upward view of the fish-egg embryo heartbeat observation device of the present embodiment
As Fig. 1, fish-egg embryo heartbeat observation device 100 comprises: accomodating unit 10, thermostatted water flow unit 20, thermostatted water flow unit 10 is connected with accomodating unit side plate.
Fig. 2 is the front view of the fish-egg embryo heartbeat observation device of the present embodiment
As shown in Figure 2, wherein,
Accomodating unit 10 comprises upper cover plate 11, lower shoe 12, side plate 13 and is positioned at the chamber 14 of accomodating unit 10, accomodating unit 10 profile is square, corresponding upper cover plate 11, lower shoe 12 are also square, and in this embodiment, upper cover plate 11, lower shoe 12 be printing opacity all;
Side plate 13 between upper cover plate 11 and lower shoe 12, and is connected with lower shoe 12, and chamber quantity is four;
Chamber 14 is cylindrical, chamber internal diameter 2cm, degree of depth 2cm, and chamber 14 wall surrounding is provided with two exchanging water holes 141, and between chamber 14 and side plate 13, chamber 14 all leaves space 15 each other, and space 15 is connected, for holding thermostat flow water;
Exchanging water hole 141 on chamber 14 comprises a high-order exchanging water hole 141 (a), a low level exchanging water hole 141 (b), high-order exchanging water hole 141 (a) is crisscross arranged with low level exchanging water hole 141 (b): high-order exchanging water hole 141 (a) is arranged at the top of chamber 14 wall surrounding, and low level exchanging water hole 141 (b) is arranged at the bottom of chamber 14 wall surrounding.In the present embodiment, exchanging water hole footpath is 3mm;
Thermostatted water flow unit 20 comprises inlet opening 21, apopore 22;
Inlet opening 21, apopore 22 are arranged on accomodating unit side plate 13, and are communicated with side plate 13, and water body flows to from inlet opening 21, flow out from apopore 22, for fish-egg embryo heartbeat observation device 100 provides flowing thermostatted water.
Fig. 3 is the left view of middle fish-egg embryo heartbeat observation device of the present invention.
As shown in Figure 3, wherein, coma 142 is provided with in chamber 14, in the present embodiment, coma adopts printing opacity chemical fibre, for cushioning the impact that water body flow causes chamber 14, the bottom lay of chamber 14 has fiber batt layer 143, in the present embodiment, fiber batt layer 143 adopts absorbent cotton thin layer, for fixing fish-egg embryo.
In addition, upper cover plate 11 has not erasable mark (not shown in FIG.), mark shape size is consistent along shape size with on cavity, facilitates coma 142 positioning bonding on upper cover plate 11.
Using method
In enforcement, first the mark bonding be arranged on printing opacity upper cover plate 11 inwall is fixed the coma 142 (the present embodiment adopts printing opacity chemical fibre) of suitable size, shape.
Again sampling pump one end is connected constant temperature water source, the sampling pump other end connects inlet opening 21, under sampling pump effect, thermostatted water is intake by inlet opening 21, enter accomodating unit 10, and be full of between side plate 13 and chamber 14 and in chamber 14 space 15 each other, and be full of each chamber 14, then flowed out by apopore 22.
In chamber 14, water body flows to low level exchanging water hole 141 (b) from high-order exchanging water hole 141 (a), realizes changing water, for providing oxygen in chamber 14 in chamber 14.
Control hydraulic pressure, after keeping chamber 14 stable level, by suction pipe, ovum embryo is put into bottom chamber 14, repave fiber batt layer 143 (in the present embodiment, fiber batt layer adopts absorbent cotton thin layer), fish-egg embryo is made to be fixed on fiber batt layer 143, and cover the upper cover plate 11 of the coma 142 that bondd, with closed chamber 14, along with upper cover plate 11 covers, coma 142 accurately inserts fiber batt layer 143, and filled chamber 14, afterwards this device is positioned on inverted microscope, by the situation of the lower shoe observation fish-egg embryo heartbeat of printing opacity.
One as the present embodiment is out of shape, above-mentioned accomodating unit 10 can also be in circular, ellipse any one, be ensure implementation result, chamber quantity is no more than 16, and exchanging water hole diameter range is 2-4mm.
The present embodiment effect and effect
According to the present embodiment fish-egg embryo heartbeat observation device, the equal printing opacity of coma of filling in upper cover plate, lower shoe and chamber, for chamber provides certain light source, for observation provides light condition;
Use middle upper cover plate to cover, fish-egg embryo heartbeat observation device is closed, prevents outside contamination;
Thermostatted water continues flowing, for the device provides stable environmental temperature, and brings in accomodating unit by the oxygen in air, and the water body of band oxygen is flowed to low level exchanging water hole by the high-order exchanging water hole on chamber, for providing the oxygen that fish-egg embryo needs in chamber;
Exchanging water hole footpath is less, make water body flow in chamber more slow, add the coma of filling in upper chamber and cushioned the impact that water body flow causes chamber, the fiber batt layer of cavity bottom fixes fish-egg embryo, prevents the accuracy due to fish-egg embryo effect of jitter observed result in observation generally.
Although be described the embodiment of specification of the present invention above; so that those skilled in the art understand the present invention; but should be clear; the invention is not restricted to the scope of embodiment; to those skilled in the art; as long as various change to limit and in the spirit and scope of the present invention determined, these changes are apparent, and all innovation and creation utilizing the present invention to conceive are all at the row of protection in appended claim.
Claims (10)
1. a fish-egg embryo heartbeat observation device, for placing the fish-egg embryo needing observation, is characterized in that, comprise:
Accomodating unit, for holding described fish-egg embryo, comprises the upper cover plate of printing opacity, lower shoe, and the side plate be connected between described upper cover plate, described lower shoe and with described lower shoe;
Thermostatted water flow unit, comprises inlet opening, apopore, and described inlet opening, described apopore are arranged on described accomodating unit, and are communicated with described side plate;
Wherein, in described accomodating unit, be provided with at least one chamber, described chamber is provided with exchanging water hole, between described chamber and described side plate, between described chamber and described chamber, all leave space.
2. fish-egg embryo heartbeat observation device according to claim 1, is characterized in that:
Wherein, described exchanging water hole, comprises at least one pair of be staggered high-order exchanging water hole and low level exchanging water hole of arranging,
Described high-order exchanging water hole is arranged at the top of described chamber, and described low level exchanging water hole is arranged at the bottom of described chamber.
3. fish-egg embryo heartbeat observation device according to claim 1, is characterized in that:
Wherein, the diameter 2-4mm of described exchanging water hole.
4. fish-egg embryo heartbeat observation device according to claim 1, is characterized in that:
Wherein, in described chamber, be filled with coma, for cushioning the impact that water body flow causes described chamber.
5. fish-egg embryo heartbeat observation device according to claim 4, is characterized in that:
Wherein, described coma is bonding with described upper cover plate,
The position that described upper cover plate is connected with described coma, has not erasable mark, and the shape of described mark is consistent along shape with on described chamber inner wall, the size of the shape of described mark be less than described chamber inner wall on along size.
6. fish-egg embryo heartbeat observation device according to claim 4, is characterized in that:
Wherein, described coma is printing opacity group.
7. fish-egg embryo heartbeat observation device according to claim 1, is characterized in that:
Wherein, the bottom lay of described chamber has fiber batt layer, for fixing described fish-egg embryo.
8. fish-egg embryo heartbeat observation device according to claim 1, is characterized in that:
Wherein, described inlet opening and described apopore are crisscross arranged on described accomodating unit.
9. fish-egg embryo heartbeat observation device according to claim 1, is characterized in that:
Wherein, the profile of described accomodating unit be square, in circular, ellipse any one.
10. fish-egg embryo heartbeat observation device according to claim 1, is characterized in that:
Wherein, described chamber shape be cylinder, internal diameter 2cm, degree of depth 2cm, the quantity 4-16 of described chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610019079.1A CN105557614B (en) | 2016-01-13 | 2016-01-13 | Fish-egg embryo heartbeat observation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610019079.1A CN105557614B (en) | 2016-01-13 | 2016-01-13 | Fish-egg embryo heartbeat observation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105557614A true CN105557614A (en) | 2016-05-11 |
| CN105557614B CN105557614B (en) | 2018-04-20 |
Family
ID=55868739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610019079.1A Expired - Fee Related CN105557614B (en) | 2016-01-13 | 2016-01-13 | Fish-egg embryo heartbeat observation device |
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| CN (1) | CN105557614B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108924493B (en) * | 2018-07-09 | 2020-10-20 | 浙江省海洋水产研究所 | Device and method for monitoring vertical distribution of marine fish eggs |
Citations (6)
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| JP2005278563A (en) * | 2004-03-30 | 2005-10-13 | Gex Corp | Water tank |
| CN102297865A (en) * | 2011-05-27 | 2011-12-28 | 宁波大学 | Biological water quality monitoring system by fish behaviors and monitoring method thereof |
| CN202799890U (en) * | 2012-08-23 | 2013-03-20 | 浙江省海洋开发研究院 | Thermostatic fish tank |
| CN202857565U (en) * | 2012-10-25 | 2013-04-10 | 苍南县澳鑫海洋生物科技有限公司 | Sea cucumber cultivation device used for raising northern sea cucumber in south |
| CN103392636A (en) * | 2013-05-03 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | Experimental apparatus for seawater shellfish physiology research and using method thereof |
| CN204742227U (en) * | 2015-07-17 | 2015-11-11 | 李倩 | Novel jar is raised to jellyfish |
-
2016
- 2016-01-13 CN CN201610019079.1A patent/CN105557614B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005278563A (en) * | 2004-03-30 | 2005-10-13 | Gex Corp | Water tank |
| CN102297865A (en) * | 2011-05-27 | 2011-12-28 | 宁波大学 | Biological water quality monitoring system by fish behaviors and monitoring method thereof |
| CN202799890U (en) * | 2012-08-23 | 2013-03-20 | 浙江省海洋开发研究院 | Thermostatic fish tank |
| CN202857565U (en) * | 2012-10-25 | 2013-04-10 | 苍南县澳鑫海洋生物科技有限公司 | Sea cucumber cultivation device used for raising northern sea cucumber in south |
| CN103392636A (en) * | 2013-05-03 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | Experimental apparatus for seawater shellfish physiology research and using method thereof |
| CN204742227U (en) * | 2015-07-17 | 2015-11-11 | 李倩 | Novel jar is raised to jellyfish |
Non-Patent Citations (1)
| Title |
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| 黄凯 等: "水溶性CdTe量子点对斑马鱼胚胎发育的影响", 《环境科学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108924493B (en) * | 2018-07-09 | 2020-10-20 | 浙江省海洋水产研究所 | Device and method for monitoring vertical distribution of marine fish eggs |
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| Publication number | Publication date |
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| CN105557614B (en) | 2018-04-20 |
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Granted publication date: 20180420 Termination date: 20220113 |