CN105548082A - Double-wave-length nephelometry measuring device - Google Patents
Double-wave-length nephelometry measuring device Download PDFInfo
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- CN105548082A CN105548082A CN201610060704.7A CN201610060704A CN105548082A CN 105548082 A CN105548082 A CN 105548082A CN 201610060704 A CN201610060704 A CN 201610060704A CN 105548082 A CN105548082 A CN 105548082A
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- China
- Prior art keywords
- nephelometry
- wave
- light source
- lens
- cup
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
Abstract
The invention provides a double-wave-length nephelometry measuring device, and relates to the technical field of a human body fluid detection instrument. The double-wave-length nephelometry measuring device comprises a nephelometry cup, wherein two sets of light source emitting systems A with different wave lengths are symmetrically arranged at the two sides of the nephelometry cup along the optical axial line; two sets of scattering light signal receiving conversion systems S are symmetrically arranged in 30-degree to 60-degree directions at the two sides of the optical axial line; each light source emitting system A uses a light source for emitting light beams; the light beams sequentially pass through a collimating lens, a focusing lens and the nephelometry cup, and then, the transmission light beams are stopped at a trapped wave sheet; according to the scattering light signal receiving conversion systems S, scattering light signals passing through the nephelometry cup sequentially pass through a collecting lens, a diaphragm and an interference filter, and are then converted into electric signals through a photoelectric detector. The double-wave-length light source is used for nephelometry; the external interference can be reduced; the detection sensitivity and the detection result accuracy can be improved. The detection system has the advantages that the structure is compact; the specific detection items can be completed through changing the light source wave length; the practicability is high; the double-wave-length nephelometry measuring device can be widely applied to various kinds of nephelometry detection equipment.
Description
Technical field
The present invention relates to human body fluid detecting instrument technical field, particularly a kind of dual wavelength scattering turbidimetry measurement mechanism.
Background technology
In modern medicine inspection technology, optics turbidimetry is widely used in the detection of all kinds of material.Optics turbidimetry is to measure a kind of analytical approach of material composition content according to the transmitted light of suspension or the intensity of scattered light.When light is by a turbid solution, because suspension selectively absorbs a part of luminous energy, and suspension phase all directions scattering another part light, reduce the intensity of transmitted light, the relation object of its penetrability and suspended sediment concentration is similar to Lambert-Beer's law.The concentration of suspension can be reflected according to the change of penetrability.The turbid device of each analogy of current use, scattered light urbidmetry and turbidimetry respectively have quality, wherein turbidimetry structure is simple, can be applicable to easily in various types of detecting instrument, but its sensitivity and precision are all not ideal enough, and required test sample amount is large, sense cycle is long; Scattered light urbidmetry has higher sensitivity and precision, and required sample size is less, but due to scattered signal more weak, be easily subject to external interference, reduce the accuracy of detection.
Summary of the invention
The object of this invention is to provide a kind of dual wavelength scattering turbidimetry measurement mechanism that can reduce external interference, improve sensitivity and the testing result accuracy detected.
The object of the invention is to be realized by following approach:
Dual wavelength scattering turbidimetry measurement mechanism, comprise than turbid cup, the light source emission coefficient A of two cover different wave lengths is being housed along optical axis symmetry than turbid cup both sides, in 30 °-60 ° direction symmetries of optical axis both sides, two cover scattered light signals are housed and receive conversion system S, described light source emission coefficient A is by source emissioning light bundle, successively through collimation lens, condenser lens, than turbid cup after, transmitted light beam end in trap sheet composition; Described scattered light signal receive conversion system S be by through than the scattered light signal after turbid cup successively after collecting lens, diaphragm, interference filter element, be converted into electric signal composition by photodetector.
Be further described light source be semiconductor laser.
Be further described collimation lens be aspheric mirror, described condenser lens is plano-convex lens, and described collecting lens is convex lens.
It is different to be further that two cover scattered light signals that described symmetry is equipped with receive the aperture of diaphragm in conversion system S.
Good effect of the present invention: dual wavelength scattering turbidimetry measurement mechanism of the present invention adopts double-wavelength light source to carry out scattering turbidimetry, external interference can be reduced, improve the sensitivity of detection, increase the accuracy of testing result, this detection system compact conformation, specific test item can be completed by changing optical source wavelength, practical, can be widely used in the turbid checkout equipment of each analogy.
Accompanying drawing explanation
Fig. 1 is light path principle structural representation of the present invention
Fig. 2 is that the present invention is for full-automatic blood coagulation analyzer structural representation
In figure: 100.a semiconductor laser, 101.a collimation lens, 102.a focus lamp, 103.a trap sheet, 200.b semiconductor laser, 201.b collimation lens, 202.b focus lamp, 203.b trap sheet, 300. than turbid cup, 110.s1 collecting lens, 111.s1 diaphragm, 112.s1 interference filter element, 113.s1 photodetector, 114.s2 collecting lens, 115.s2 diaphragm, 116.s2 interference filter element, 117.s2 photodetector, 210.d1 collecting lens, 211.d1 diaphragm, 212.d1 interference filter element, 213.d1 photodetector, 214.d2 collecting lens, 215.d2 diaphragm, 216.d2 interference filter element, 217.d2 photodetector, 10. sample system, 11. sample positions, 12. sample application pins, 20. reagent systems, 21. reagent positions, 22. reagent pipetting volume pins, 30. reactive systems, 32. puddlers, 33. cleaning stations, 40. dual wavelength scattering turbidimetry detection systems
Embodiment
Below in conjunction with Figure of description, the present invention is elaborated:
As shown in Figure 1, dual wavelength scattering turbidimetry measurement mechanism of the present invention overlaps light source emission coefficient A and the scattered light signal reception conversion system S of different wave lengths by two and forms than turbid cup, the light source emission coefficient A of two cover different wave lengths is being housed along optical axis symmetry than turbid cup 300 both sides, is wherein respectively overlapping light source emission coefficient A and be made up of light source, collimation lens, focus lamp and trap sheet respectively; In 30 °-60 ° direction symmetries of optical axis both sides, two cover scattered light signals are housed and receive conversion system S, wherein respectively overlap scattered light signal reception conversion system S and be made up of collecting lens, diaphragm, interference filter element and photodetector respectively.
Wherein in a set of light path, light source is a semiconductor laser 100, after a collimation lens 101, light beam becomes parallel circular light spot, again through a focus lamp 102, pass through than turbid cup 300 after light beam is focused on, its transmitted light terminates on a trap sheet 103, and pass through than turbid cup 300 after being focused on by light beam, its transmitted light terminates on a trap sheet 103; In the another set of light path that symmetry is equipped with, light source is b semiconductor laser 200, and after b collimation lens 201, light beam becomes parallel circular light spot, then through b focus lamp 202, and after being focused on by light beam, by than turbid cup 300, its transmitted light terminates on b trap sheet 203.When light beam irradiation than on turbid cup 300 time, due to detect solution generation turbidity change, have suspension produce, cause light beam generation scattering.Receive on conversion system S at scattered light signal, be divided into again two-way S1 and S2 to carry out collection respectively to receive, in 30 °-60 ° direction symmetries of optical axis both sides, two cover scattered light signals are housed and receive conversion system S, the receiving angle of selection is a certain fixed angle in 30 °-60 °.Scattered light S1, after s1 collecting lens 110, by a circular aperture s1 diaphragm 111, then by after s1 interference filter element 112 filtering, adopts s1 photodetector 113 to change scattered light into electric signal; Equally, scattered light S2, after s2 collimation lens 114, by a circular aperture s2 diaphragm 115, then by after s2 interference filter element 116 filtering, adopts s2 photodetector 117 to change scattered light into electric signal.Another set of scattered light signal receives on conversion system S, be divided into again two-way D1 and D2 to carry out collection respectively to receive, in 30 °-60 ° direction symmetries of optical axis both sides, two cover scattered light signals are housed and receive conversion system S, the receiving angle of selection is a certain fixed angle in 30 °-60 °.Scattered light D1, after d1 collecting lens 210, by a circular aperture d1 diaphragm 211, then by after d1 interference filter element 212 filtering, adopts d1 photodetector 113 to change scattered light into electric signal; Equally, scattered light D2, after d2 collimation lens 214, by a circular aperture d2 diaphragm 215, then by after d2 interference filter element 216 filtering, adopts d2 photodetector 217 to change scattered light into electric signal.Symmetrical identical on light channel structure, it is arranged in the other direction, just select the semiconductor laser of different wave length, its corresponding various optical device optical characteristics also needs corresponding change, the elimination that trap sheet is corresponding or weaken the transmitted light beam energy of this road wavelength, reduces its impact on the semiconductor laser of another wavelength.Be preferably square structure than turbid cup 300 shape, the less change to propagation path of can trying one's best, its material can select glass material, also can select plastic material.Article two, the s1 diaphragm 111 on scattering light path S1 and S2 and s2 diaphragm 115 pore size different, wider sensing range can be obtained by simple mathematics seletion calculation when being convenient to rear end computing, reduce photodetector saturated probability.
As shown in Figure 2, it is a kind of specific embodiment of the present invention, this pick-up unit is full-automatic blood coagulation analyzer, the arrangement of this apparatus structure is similar to automatic clinical chemistry analyzer, primary structure is made up of sample system 10, reagent system 20, reactive system 30 and detection system 40, and all the other also have hardware system and software systems.Comprise multiple sample position 11 and a sample application pin 12 in sample system 10, the present embodiment adopts double sample arrangement, altogether can place 40 samples, and this sample disk can clockwise and be rotated counterclockwise.Reagent system 20 is made up of multiple reagent position 21 and a reagent pipetting volume pin 22, and the present embodiment adopts can place 32 kits altogether, and this reagent disc also can clockwise and be rotated counterclockwise, and it is with constant temperature refrigerating plant.Reactive system 30 comprises multiple than turbid cup 300, puddler 32 and a cleaning station 33, the present embodiment can place 60 altogether than turbid cup 300, this reaction tray also can clockwise and be rotated counterclockwise, it is with 37 DEG C of thermostats, and its cleaning station 33 can clean successively than turbid cup and damping fluid of annotating.Detection system 40 is dual wavelength scattering turbidimetry measurement mechanism of the present invention, and light source selects semiconductor laser, and power is 5mw, selects a semiconductor laser 100 wavelength to be 660nm, selects b semiconductor laser 200 wavelength to be 405nm, adopts Constant-power drive mode; Laser collimation lens selects aspheric mirror; Condenser lens is chosen as plano-convex lens; The cutoff range selecting a trap sheet 103 is 600nm-700nm, and the cutoff range selecting b trap sheet 203 is 350nm-500nm, and trap rate is greater than 85%; The collecting lens that scattered light signal receives in conversion system S is chosen as convex lens; The pore size selecting s1 diaphragm 111 is 3mm, and the pore size selecting s2 diaphragm 115 is 1.5mm; Select s1 interference filter element 112 centre wavelength to be 660nm, select s2 interference filter element 116 centre wavelength to be 405nm, half-wave is wide is 10nm; Silicon photodetector selected by photodetector, adopts photovoltaic mode duty.
Claims (4)
1. a dual wavelength scattering turbidimetry measurement mechanism, comprise than turbid cup, it is characterized in that at the light source emission coefficient A that two cover different wave lengths are housed along optical axis symmetry than turbid cup both sides, in 30 °-60 ° direction symmetries of optical axis both sides, two cover scattered light signals are housed and receive conversion system S, described light source emission coefficient A is by source emissioning light bundle, successively through collimation lens, condenser lens, than turbid cup after, transmitted light beam end in trap sheet composition; Described scattered light signal receive conversion system S be by through than the scattered light signal after turbid cup successively after collecting lens, diaphragm, interference filter element, be converted into electric signal composition by photodetector.
2. dual wavelength scattering turbidimetry measurement mechanism according to claim 1, is characterized in that described light source is semiconductor laser.
3. dual wavelength scattering turbidimetry measurement mechanism according to claim 1, it is characterized in that described collimation lens is aspheric mirror, described condenser lens is plano-convex lens, and described collecting lens is convex lens.
4. dual wavelength scattering turbidimetry measurement mechanism according to claim 1, is characterized in that two cover scattered light signals that described symmetry is equipped with receive the aperture of diaphragm in conversion system S different.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610060704.7A CN105548082A (en) | 2016-01-28 | 2016-01-28 | Double-wave-length nephelometry measuring device |
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| CN201610060704.7A CN105548082A (en) | 2016-01-28 | 2016-01-28 | Double-wave-length nephelometry measuring device |
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| CN105548082A true CN105548082A (en) | 2016-05-04 |
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| CN201610060704.7A Pending CN105548082A (en) | 2016-01-28 | 2016-01-28 | Double-wave-length nephelometry measuring device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732134A (en) * | 2018-07-18 | 2018-11-02 | 上海原科实业发展有限公司 | A kind of scattering of multi-wavelength and transmittance opacimetry device |
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| CN201673117U (en) * | 2010-05-28 | 2010-12-15 | 天津美德太平洋科技有限公司 | Analysis instrument measuring head and measuring cup for multispectral scattering and transmission turbidimetry detection |
| CN102288581A (en) * | 2011-07-29 | 2011-12-21 | 南京诺尔曼生物技术有限公司 | Specific protein measuring method and device |
| CN102288580A (en) * | 2011-05-09 | 2011-12-21 | 南开大学 | Protein turbidity comparator with double light sources and four passages |
| CN202339325U (en) * | 2011-07-27 | 2012-07-18 | 南京诺尔曼生物技术有限公司 | Scattering turbidimetry measurement apparatus |
| CN104596990A (en) * | 2015-01-23 | 2015-05-06 | 中国农业大学 | Two-channel optical fiber method and sensor for measuring turbidity |
| CN205538673U (en) * | 2016-01-28 | 2016-08-31 | 广州埃克森生物科技有限公司 | Dual wavelength scattering is than turbid measuring device |
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2016
- 2016-01-28 CN CN201610060704.7A patent/CN105548082A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201673117U (en) * | 2010-05-28 | 2010-12-15 | 天津美德太平洋科技有限公司 | Analysis instrument measuring head and measuring cup for multispectral scattering and transmission turbidimetry detection |
| CN102288580A (en) * | 2011-05-09 | 2011-12-21 | 南开大学 | Protein turbidity comparator with double light sources and four passages |
| CN202339325U (en) * | 2011-07-27 | 2012-07-18 | 南京诺尔曼生物技术有限公司 | Scattering turbidimetry measurement apparatus |
| CN102288581A (en) * | 2011-07-29 | 2011-12-21 | 南京诺尔曼生物技术有限公司 | Specific protein measuring method and device |
| CN104596990A (en) * | 2015-01-23 | 2015-05-06 | 中国农业大学 | Two-channel optical fiber method and sensor for measuring turbidity |
| CN205538673U (en) * | 2016-01-28 | 2016-08-31 | 广州埃克森生物科技有限公司 | Dual wavelength scattering is than turbid measuring device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108732134A (en) * | 2018-07-18 | 2018-11-02 | 上海原科实业发展有限公司 | A kind of scattering of multi-wavelength and transmittance opacimetry device |
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Application publication date: 20160504 |
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