CN102288580A - Protein turbidity comparator with double light sources and four passages - Google Patents
Protein turbidity comparator with double light sources and four passages Download PDFInfo
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- CN102288580A CN102288580A CN2011101175071A CN201110117507A CN102288580A CN 102288580 A CN102288580 A CN 102288580A CN 2011101175071 A CN2011101175071 A CN 2011101175071A CN 201110117507 A CN201110117507 A CN 201110117507A CN 102288580 A CN102288580 A CN 102288580A
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Abstract
The invention relates to the field of chemical analysis, and specifically relates to a scattering turbidity detecting device with double light sources and four passages in protein analysis. The device comprises a light path part, a light detecting part and a data processing and displaying part; the light path part comprises two tail fiber laser light sources with different wavelengths, two 1*4 fiber branching units, a fiber collimator and a sample pool; light emitted from the tail fiber light sources is directly sent into the 1*4 fiber branching units; four parts of same light are output, are collimated by the fiber collimator, and then are injected into the sample pool; emergent scattered light is received and detected by the light detecting part; an analog signal is input into the data processing and displaying part; and after the analog signal is processed, a detection result is displayed on a liquid crystal display. As the device comprises the laser light sources with the different wavelengths, the range of samples which can be detected by the instrument is wider, and simultaneously, the detection result of the samples is more accurate by adopting different light sources. The incident light is divided into the same four parts by using the fiber branching units, so that four same or different samples can be detected simultaneously by the instrument, and the detection efficiency is improved greatly. The device is simple and feasible; the system error is small; and the operation is simple and convenient.
Description
Technical field
The present invention relates to chemical analysis field, be specifically related to the nephelometric turbidity pick-up unit of the two light source four-ways in analysis of protein.
Technical background
The immunologic assay of protein is from quantitative sedimentation in the liquid medium.Use scattering turbidimetry method can be measured the protein in the solution fast and accurately.So-called " scattering turbidimetry method " is to survey scattered light intensity according to the light scattering effect of Ag-Ab in the solution (Ag-Ab) mix suspension grain.When the light of certain wavelength ran into the Ag-Ab compound by solution, light deflected, and the distribution of deflection is relevant with the size of lambda1-wavelength and effects of ion.The concentration of scattered intensity and Ag-Ab compound is directly proportional and the scattering angle is directly proportional and lambda1-wavelength is inversely proportional to.Use laser nephelometer, assay method has rate method and end-point method.The speed turbidimetry is the maximum reaction velocity of measuring in antibody and the antigen association reaction process, the peak value when promptly reaction reaches the peak.Wherein speed is meant the association rate of Ag-Ab in the unit interval.The peak value of reaction is in tens seconds, and the height of peak value is directly proportional with the amount of antigen, and the affinity of the time that peak value occurs and the concentration of antibody, Ag-Ab is directly related.Here use the rate scattering turbidimetry.Terminal point scattering turbidimetry method is to measure when the Ag-Ab reaction reaches balance, generally forms back effect a period of time at compound, is generally 30-60 minute.This moment, the turbidity of compound no longer was subjected to the influence of time, but must carry out turbidimetric analysis turbidimetry before the compound polymerization produces flocculent deposit.The present invention uses the rate scattering turbidimetry, and detection time is short, the efficient height, and the result is accurate.
Existing albumen is than sample cell of a turbid instrument light source irradiation of most use, and detection efficiency is low; The hyperchannel albumen of minority uses a plurality of light sources than turbid instrument, sample cell of each light source irradiation has certain processing means of needs to make each sample cell not be subjected to the influence of other light sources simultaneously, so causes light path part very loaded down with trivial details, bulky, use to lack dirigibility; Some uses composite light source than turbid instrument, by using different optical filters to obtain the incident light of different wave length, because light source is not laser, need behind optical filter, add optical device such as lens with assurance incident light intensity, make the light path part of instrument huge equally, loaded down with trivial details.
Summary of the invention
The object of the present invention is to provide a kind of tail optical fiber LASER Light Source of different wave length of can using to detect two light source four-way albumen of a plurality of protein examples simultaneously than turbid instrument.
For achieving the above object, overcome the deficiencies in the prior art, the technical scheme that the present invention uses is:
The present invention includes light path part, optical detection part branch and data processing and display part, light path part comprises the tail optical fiber LASER Light Source of two different wave lengths, two 1x4 optical fiber splitters, optical fiber collimator, sample cell, the light of tail optical fiber lasing light emitter emission is directly sent into the 1x4 optical fiber splitter, exporting four parts of same light is collimated by optical fiber collimator, inject sample cell, the scattered light of outgoing divides reception to detect by optical detection part, analog input data signal is handled and the display part, on LCD, shown the result who detects after treatment.
The tail optical fiber laser instrument of described two different wave lengths links to each other respectively at the optical fiber splitter of two 1x4, the output terminal of the optical fiber splitter of 1x4 links to each other with optical fiber collimator, and the optical fiber splitter of two 1x4 by collimating apparatus has a branch of emergent light in four sample cells respectively; Described two tail optical fiber laser instruments, the wavelength difference of emission light wave can be distinguished separately and use, and directly be connected with a 1x4 optical fiber splitter respectively; Described two 1x4 optical fiber splitters can be divided into tail optical fiber LASER Light Source emitted laser four bundles of identical or any splitting ratio, inject sample cell after being handled by optical fiber collimator; Described sample cell has four, and each sample cell all over against two optical fiber collimators are arranged, is exported a branch of emergent light of the optical fiber splitter of two 1x4 respectively.
The present invention has the following advantages:
1, the tail optical fiber LASER Light Source of different wave length detects simultaneously to a plurality of samples, and measurement result is accurate, and the light path part optical loss is little, and volume is little, and the detection efficiency height can satisfy multiple detection demand;
2, measurement result is accurate.Use the tail optical fiber LASER Light Source of two different wave lengths, the sample that the present invention can be detected is wider general, adopts Different Light simultaneously, and it is more accurate to test result of samples to make;
3, the light path part optical loss is little, and volume is little.Use the tail optical fiber LASER Light Source, output light directly feeds the optical fiber splitter of 1x4, and output light is directly injected sample cell after being collimated by optical fiber collimator, has saved optical channel, thereby has reduced optical loss, and the light path part volume is greatly reduced;
4, detection efficiency height.Use the optical fiber splitter of 1x4, incident laser is divided into four of identical or any splitting ratio restraints light, feed in four sample cells, can detect four samples simultaneously, than turbid instrument, detection efficiency improves greatly compared with single pass;
5, can satisfy multiple detection demand.The user can change the optical fiber splitter of the 1x4 of different splitting ratios, makes the ratio of four bundle emergent lights satisfy the demand of using.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Embodiment
As shown in Figure 1, the present invention includes light path part, optical detection part branch and data processing and display part, light path part comprises the tail optical fiber LASER Light Source of two different wave lengths, two 1x4 optical fiber splitters, optical fiber collimator, sample cell, the light of tail optical fiber lasing light emitter emission is directly sent into the 1X4 optical fiber splitter, exporting four parts of same light is collimated by optical fiber collimator, inject sample cell, the scattered light of outgoing divides reception to detect by optical detection part, and analog input data signal is handled and the display part, shows the result who detects after treatment on LCD.The tail optical fiber laser instrument of described two different wave lengths links to each other respectively at the optical fiber splitter of two 1x4, the output terminal of the optical fiber splitter of 1x4 links to each other with optical fiber collimator, and the optical fiber splitter of two 1x4 by collimating apparatus has a branch of emergent light in four sample cells respectively.Described two tail optical fiber laser instruments, the wavelength difference of emission light wave can be distinguished separately and use, and directly be connected with a 1x4 optical fiber splitter respectively; Described two 1x4 optical fiber splitters can be divided into tail optical fiber LASER Light Source emitted laser four bundles of identical or arbitrary proportion, inject sample cell after being handled by optical fiber collimator; Described sample cell has four, and each sample cell all over against two optical fiber collimators are arranged, is imported a branch of emergent light of the optical fiber splitter of two 1x4 respectively.
The course of work of the present invention is as follows:
At first sample is put into sample cell, use tail optical fiber laser instrument 1 as light source, laser directly enters optical fiber splitter 1.The laser of identical or any splitting ratio is restrainted in optical fiber splitter 1 output four, injects sample cell through after the processing of optical fiber collimator.Scattered light signal through sample cell is divided reception and output electric signal by optical detection part.Use tail optical fiber laser instrument 2 as light source afterwards, laser directly enters optical fiber splitter 2.The laser of identical or any splitting ratio is restrainted in optical fiber splitter 2 outputs four, injects sample cell through after the processing of optical fiber collimator.Scattered light signal through sample cell is divided reception and output electric signal by optical detection part.After handle electric signal process data processing that obtains for twice and display part, on LCD, show testing result.
The present invention adopts the rate scattering turbidimetry, the mode that a plurality of samples is detected simultaneously by the tail optical fiber LASER Light Source of using two different wave lengths, can detect identical or different sample in a plurality of sample cells simultaneously, realize efficiently, detect accurately protein example.Use the tail optical fiber LASER Light Source, output light directly feeds the optical fiber splitter of 1x4, and output light is directly injected sample cell after being collimated by optical fiber collimator, has saved optical channel, thereby has reduced optical loss, and the light path part volume is greatly reduced.Used the optical fiber splitter of 1x4, can detect four samples simultaneously, than turbid instrument, detection efficiency improves greatly compared with single pass.Simultaneously, the user can change the optical fiber splitter of the 1x4 of different splitting ratios, makes the ratio of four bundle emergent lights satisfy the demand of using.
Claims (4)
1. two light source four-way albumen are than turbid instrument, comprise light path part, optical detection part branch and data processing and display part, it is characterized in that: described light path part comprises the tail optical fiber LASER Light Source of two different wave lengths, two 1X4 optical fiber splitters, optical fiber collimator, sample cell; The tail optical fiber laser instrument of described two different wave lengths links to each other respectively at the optical fiber splitter of two 1x4, the output terminal of the optical fiber splitter of 1x4 links to each other with optical fiber collimator, and the optical fiber splitter of two 1x4 by collimating apparatus has a branch of emergent light in four sample cells respectively.
As 1 described pair of light source four-way of claims albumen than turbid instrument, it is characterized in that, described two tail optical fiber laser instruments, the wavelength difference of emission light wave can be distinguished separately and to use, and directly be connected with a 1x4 optical fiber splitter respectively.
As 1 described pair of light source four-way of claims albumen than turbid instrument, it is characterized in that, described two 1x4 optical fiber splitters can be divided into tail optical fiber LASER Light Source emitted laser four bundles of identical or arbitrary proportion, inject sample cell after being handled by optical fiber collimator.
As 1 described pair of light source four-way of claims albumen than turbid instrument, it is characterized in that described sample cell has four, each sample cell all over against two optical fiber collimators are arranged, is exported a branch of emergent light of the optical fiber splitter of two 1x4 respectively.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011101175071A CN102288580A (en) | 2011-05-09 | 2011-05-09 | Protein turbidity comparator with double light sources and four passages |
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| CN2011101175071A CN102288580A (en) | 2011-05-09 | 2011-05-09 | Protein turbidity comparator with double light sources and four passages |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102914519A (en) * | 2012-10-19 | 2013-02-06 | 中国科学院合肥物质科学研究院 | Optical fiber type laser liquid turbidity measuring device and measuring method |
| CN105548082A (en) * | 2016-01-28 | 2016-05-04 | 广州埃克森生物科技有限公司 | Double-wave-length nephelometry measuring device |
| WO2020034612A1 (en) * | 2018-08-14 | 2020-02-20 | 三诺生物传感股份有限公司 | Optical detection mechanism and fully automatic biochemical analyzer |
| CN112748126A (en) * | 2019-10-31 | 2021-05-04 | 芯恩（青岛）集成电路有限公司 | Wafer detection system and detection method |
| CN112924418A (en) * | 2019-12-05 | 2021-06-08 | 深圳迈瑞生物医疗电子股份有限公司 | Specific protein analysis system |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191768A (en) * | 2006-12-01 | 2008-06-04 | 林洁丽 | Safe and on-line type laser optical fibre nephelometer |
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2011
- 2011-05-09 CN CN2011101175071A patent/CN102288580A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191768A (en) * | 2006-12-01 | 2008-06-04 | 林洁丽 | Safe and on-line type laser optical fibre nephelometer |
Non-Patent Citations (1)
| Title |
|---|
| 余鑫等: "多通道多气体光纤传感技术的研究", 《光通信技术》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102914519A (en) * | 2012-10-19 | 2013-02-06 | 中国科学院合肥物质科学研究院 | Optical fiber type laser liquid turbidity measuring device and measuring method |
| CN102914519B (en) * | 2012-10-19 | 2015-04-15 | 中国科学院合肥物质科学研究院 | Optical fiber type laser liquid turbidity measuring device and measuring method |
| CN105548082A (en) * | 2016-01-28 | 2016-05-04 | 广州埃克森生物科技有限公司 | Double-wave-length nephelometry measuring device |
| WO2020034612A1 (en) * | 2018-08-14 | 2020-02-20 | 三诺生物传感股份有限公司 | Optical detection mechanism and fully automatic biochemical analyzer |
| CN112748126A (en) * | 2019-10-31 | 2021-05-04 | 芯恩（青岛）集成电路有限公司 | Wafer detection system and detection method |
| CN112924418A (en) * | 2019-12-05 | 2021-06-08 | 深圳迈瑞生物医疗电子股份有限公司 | Specific protein analysis system |
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Application publication date: 20111221 |