CN105547740A - Method for acquiring epidermis of plant leaf - Google Patents
Method for acquiring epidermis of plant leaf Download PDFInfo
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- CN105547740A CN105547740A CN201510915822.7A CN201510915822A CN105547740A CN 105547740 A CN105547740 A CN 105547740A CN 201510915822 A CN201510915822 A CN 201510915822A CN 105547740 A CN105547740 A CN 105547740A
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- epidermis
- plant leaf
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Biochemistry (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a method for acquiring the epidermis of a plant leaf, belonging to the field of biotechnology. The method comprises a step of mashing the epidermis of one side of the plant leave and all the mesophyll tissue so as to obtain the epidermis of the other side of the plant leave. The invention has the beneficial effect that the method is simple and highly efficient and has a wide application scope.
Description
Technical field
The present invention relates to a kind of method obtaining plant leaf blade epidermis, belong to biological technical field.
Background technology
The epidermis of plant leaf blade is the nascent protective tissue covering blade surface, has dividing of upper and lower epidermis.The epidermis of plant leaf blade forms primarily of epidermal cell, epidermal hair, water trap and stomatal apparatus usually.That the epidermal cell shape of different plant and size, cuticle thickness, epidermal hair have that it's too late is how many, the number of water trap size and how many, stomatal apparatus, size and arrangement etc. are all different, be plant classification according to one of.
The supracutaneous stomatal apparatus of plant leaf blade controls rising and that photosynthesis two is important plant physiological processes, the opening and closing of pore, distribution, size, how many and growth course, is very important for the adaptation situation studying plant environment to external world.Therefore, obtaining plant leaf blade epidermis is contribute to very much Scientific Research and Teaching work.
The existing method obtaining plant leaf blade epidermis has: directly tear follow the example of, liquid method of emanating, scanning electron microscopy, vane combustion method and cellophane method etc.Directly tear that to follow the example of be directly tear with tweezers to get a small pieces blade epidermis in the upper of blade or lower epidermis, its shortcoming is not easily tear to get and to tear the blade epidermis got little; Segregation liquid method obtains blade epidermis with chemical agent dissolves mesophyll tissue, and its shortcoming is high, the consuming time length of cost, complex operation; Scanning electron microscopy is first with after a series of chemical reagent process, then observes by scanning electron microscope, and its shortcoming is that scanning electron microscope is expensive, the processing time is long; Vane combustion method is that its upper and lower epidermis separates voluntarily, obtains the upper and lower epidermis of blade, and its shortcoming is for being only applicable to highly lignified blade by after highly lignified vane combustion, excessively water; Cellophane method glues with normal transparent adhesive tape the upper and lower epidermis getting blade, scrapes off mesophyll cell, obtain blade epidermis, and its shortcoming is that the blade epidermis obtained is stained with glue and affects follow-up observation.
1, Duan Yunfeng, etc. a kind of simple and easy method and application thereof obtaining blade epidermis observation pore. North China agronomy report, 2008,23 (supplementary issue): 73-76.
2, Zhang Lirong, etc. the upper and lower epidermis new method of separating plant blade. physiology is circulated a notice of, and 2009,44 (12): 49-51.
3, Wei Cunxu, etc. the observation and comparison of rough plant leaf blade epidermis flaking method. biology magazine, 2008,25 (2): 10-13.
4, Wu Qinghan, etc. plant height lignification epidermal leaf cells preparation method. Botany Gazette, 2012,47 (4): 422-426.
5, Shi Changhai, etc. the sample preparation methods improvement of Leaf-Blade of Poaceae epidermis stomatic observation. Plant Physiology Communications, 2010,46 (4): 395-398.
6, Zhang Zhiyun, etc. Structural Features of Leaf Epidermis In Oryza and Phylogenetic Implications thereof. Acta Phytotaxonomica Sinica, 1998,36:8-18.
Summary of the invention
The present invention, by new acquisition plant leaf blade epidermis method, solves the problems referred to above.
The invention provides a kind of method obtaining plant leaf blade epidermis, described method is wherein an epidermis and whole mesophyll tissue of plant leaf blade are smashed to pieces, obtains the another side epidermis of plant leaf blade.
Plant leaf blade of the present invention is preferably the blade of monocotyledonous blade or dicotyledon.
Monocotyledonous blade of the present invention is preferably the blade of the blade of Chinese sorghum, the blade of corn, the blade of paddy rice, the blade of wheat, the blade of barley, the blade of lily, hyacinthine blade, the blade of tulip, the blade of onion, the blade of iris or iris.
The blade of dicotyledon of the present invention is preferably the blade of silk tree, the blade of Euonymus japonicus, the blade of little leaf boxwood, the blade of crape myrtle, the blade of the rose of Sharon, the blade of cloves, the blade of flowering plum, the blade of Chinese pagoda tree, the blade of oriental plane tree, the blade of purple Ye little Tiller, the blade of Chinese scholar tree, the blade of acer monoes, the blade of the capsule of weeping forsythia, the blade of winter jasmine, the blade of Chinese white poplar, the blade of cherry tree, the blade of peach, the blade of Japanese plum, the blade of jujube tree, the blade of pear tree, the blade of thornbuss, the blade of apricot, blade viny, the blade of blueberry, the blade of apple, the blade of walnut, the blade of Chinese chestnut, the blade of oriental white oak, the blade of Quercus acutissima, the blade of the late cherry of Japan, the blade of Cathay poplar, the blade of chrysanthemum, the blade of Chinese rose, the blade of rose, the blade of soybean, the blade of sunflower, the blade of potato, the blade of cucumber, the blade of raspberry, the blade of strawberry, the blade of eggplant, the blade of capsicum, the blade of tomato, the blade of kidney bean, the blade of willow, the blade of Luan Shu, the blade of mulberry tree, the blade of Amur honeysuckle, the blade of Chinese wax, the blade of water La Shu, the blade of safflower Chinese scholartree, the blade of Cornus controversa, the blade of persimmon, the blade of yulan, the blade of Chinese cabbage, the blade of spinach, the blade of celery, the blade of caraway, the blade of money tree, the blade of Ipomoea batatas, the blade of glossy privet, blade with a smile, the blade of Kiwi berry, the blade of radish, the blade of cotton or the blade of peanut.
Beneficial effect of the present invention is that method is simple, efficient, applied widely.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
Obtain a method for Iris Leaves lower epidermis, described method is by the epicuticle of Iris Leaves upwards, and knock to epicuticle and the fragmentation of whole mesophyll tissue, cleaning, obtains the lower epidermis of Iris Leaves.
Embodiment 2
Obtain a method for Iris Leaves epicuticle, described method is by the lower epidermis of Iris Leaves upwards, and knock to lower epidermis and the fragmentation of whole mesophyll tissue, cleaning, obtains the epicuticle of Iris Leaves.
Embodiment 3
Obtain a method for capsule of weeping forsythia blade lower epidermis, described method is by the epicuticle of capsule of weeping forsythia blade upwards, and knock to epicuticle and the fragmentation of whole mesophyll tissue, cleaning, obtains the lower epidermis of capsule of weeping forsythia blade.
Embodiment 4
Obtain a method for capsule of weeping forsythia blade epicuticle, described method is by the lower epidermis of capsule of weeping forsythia blade upwards, and knock to lower epidermis and the fragmentation of whole mesophyll tissue, cleaning, obtains the epicuticle of capsule of weeping forsythia blade.
Claims (4)
1. obtain a method for plant leaf blade epidermis, it is characterized in that: described method is wherein an epidermis and whole mesophyll tissue of plant leaf blade are smashed to pieces, obtains the another side epidermis of plant leaf blade.
2. method according to claim 1, is characterized in that: described plant leaf blade is the blade of monocotyledonous blade or dicotyledon.
3. method according to claim 2, is characterized in that: described monocotyledonous blade is the blade of the blade of Chinese sorghum, the blade of corn, the blade of paddy rice, the blade of wheat, the blade of barley, the blade of lily, hyacinthine blade, the blade of tulip, the blade of onion, the blade of iris or iris.
4. method according to claim 2, is characterized in that: the blade of described dicotyledon is the blade of silk tree, the blade of Euonymus japonicus, the blade of little leaf boxwood, the blade of crape myrtle, the blade of the rose of Sharon, the blade of cloves, the blade of flowering plum, the blade of Chinese pagoda tree, the blade of oriental plane tree, the blade of purple Ye little Tiller, the blade of Chinese scholar tree, the blade of acer monoes, the blade of the capsule of weeping forsythia, the blade of winter jasmine, the blade of Chinese white poplar, the blade of cherry tree, the blade of peach, the blade of Japanese plum, the blade of jujube tree, the blade of pear tree, the blade of thornbuss, the blade of apricot, blade viny, the blade of blueberry, the blade of apple, the blade of walnut, the blade of Chinese chestnut, the blade of oriental white oak, the blade of Quercus acutissima, the blade of the late cherry of Japan, the blade of Cathay poplar, the blade of chrysanthemum, the blade of Chinese rose, the blade of rose, the blade of soybean, the blade of sunflower, the blade of potato, the blade of cucumber, the blade of raspberry, the blade of strawberry, the blade of eggplant, the blade of capsicum, the blade of tomato, the blade of kidney bean, the blade of willow, the blade of Luan Shu, the blade of mulberry tree, the blade of Amur honeysuckle, the blade of Chinese wax, the blade of water La Shu, the blade of safflower Chinese scholartree, the blade of Cornus controversa, the blade of persimmon, the blade of yulan, the blade of Chinese cabbage, the blade of spinach, the blade of celery, the blade of caraway, the blade of money tree, the blade of Ipomoea batatas, the blade of glossy privet, blade with a smile, the blade of Kiwi berry, the blade of radish, the blade of cotton or the blade of peanut.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510915822.7A CN105547740A (en) | 2015-12-10 | 2015-12-10 | Method for acquiring epidermis of plant leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510915822.7A CN105547740A (en) | 2015-12-10 | 2015-12-10 | Method for acquiring epidermis of plant leaf |
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| Publication Number | Publication Date |
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| CN105547740A true CN105547740A (en) | 2016-05-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201510915822.7A Pending CN105547740A (en) | 2015-12-10 | 2015-12-10 | Method for acquiring epidermis of plant leaf |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106885628A (en) * | 2017-04-18 | 2017-06-23 | 大连大学 | A kind of mobile phone light measuring method for blueberry plantation |
| CN113758739A (en) * | 2021-07-20 | 2021-12-07 | 上海交通大学 | Method for collecting plant leaf living gland hair |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101482515A (en) * | 2009-01-22 | 2009-07-15 | 天津市林业果树研究所 | Tabletting method for locust stem tip chromosome |
| CN101556215A (en) * | 2009-05-13 | 2009-10-14 | 西北农林科技大学 | Sampling method for non-damage fixed position observation of plant stomata in field experiment |
| JP4448909B2 (en) * | 2004-12-03 | 2010-04-14 | 学校法人日本大学 | Sample processing system for pore measurement |
| CN101769836A (en) * | 2010-01-25 | 2010-07-07 | 河南科技大学 | Pine needle sectioning observation method |
| CN102721583A (en) * | 2012-06-01 | 2012-10-10 | 河南科技大学 | Method for producing film of leaf epidermis of stomatal pit of sweetscented oleander leaf |
-
2015
- 2015-12-10 CN CN201510915822.7A patent/CN105547740A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4448909B2 (en) * | 2004-12-03 | 2010-04-14 | 学校法人日本大学 | Sample processing system for pore measurement |
| CN101482515A (en) * | 2009-01-22 | 2009-07-15 | 天津市林业果树研究所 | Tabletting method for locust stem tip chromosome |
| CN101556215A (en) * | 2009-05-13 | 2009-10-14 | 西北农林科技大学 | Sampling method for non-damage fixed position observation of plant stomata in field experiment |
| CN101769836A (en) * | 2010-01-25 | 2010-07-07 | 河南科技大学 | Pine needle sectioning observation method |
| CN102721583A (en) * | 2012-06-01 | 2012-10-10 | 河南科技大学 | Method for producing film of leaf epidermis of stomatal pit of sweetscented oleander leaf |
Non-Patent Citations (2)
| Title |
|---|
| 张立荣 等: "分离植物叶片上、下表皮新方法", 《生物学通报》 * |
| 黄萍 等: "小花山桃草营养器官解剖结构及其生态适应性研究", 《植物研究》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106885628A (en) * | 2017-04-18 | 2017-06-23 | 大连大学 | A kind of mobile phone light measuring method for blueberry plantation |
| CN106885628B (en) * | 2017-04-18 | 2018-12-25 | 大连大学 | A kind of mobile phone light measuring method for blueberry plantation |
| CN113758739A (en) * | 2021-07-20 | 2021-12-07 | 上海交通大学 | Method for collecting plant leaf living gland hair |
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Application publication date: 20160504 |