CN105543093A - A preservative for an ammonia nitrogen degrading microbial inoculum and a preserving method thereof - Google Patents
A preservative for an ammonia nitrogen degrading microbial inoculum and a preserving method thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a preservative for an ammonia nitrogen degrading microbial inoculum. The preservative comprises water, an inhibitor, an antioxidant, a cell protective agent and inorganic salt modifiers, wherein the inhibitor is nitrite, the cell protective agent is glycerin and the inorganic salt modifiers are dihydric phosphate and hydrophosphate. The invention also discloses a preserving method of the ammonia nitrogen degrading microbial inoculum, wherein the method adopts the preservative to preserve the microbial inoculum. Compared with other preserving methods in the prior art, the preservative and the preserving method are advantageous in that 1) the preservative is advantaged by wide sources of components, low prices and relatively simpler and more convenient preservation, easy operation, no secondary pollution, and the like, and 2) during preservation of the microbial inoculum, contamination is effectively prevented and viable bacterium cells of a nitrococcus microbial inoculum are free of harm, metabolic activity is stable after preservation, the need of applying the microbial inoculum for reinforcing nitration functions in sewage treatment systems can be met, and the preservative and the preserving method are high in application value.
Description
Technical field
The present invention relates to environmental protection technical field; be specifically related to a kind of preservatives for cryopreservation ammonia nitrogen degradation microbial inoculum and store method; by the provide protection of preservatives, to reach long-time, low cost prevents ammonia nitrogen degradation microbial inoculum pollution microbes, keeps the object of nitrification activity.
Background technology
Water resources is the important component part of human environment, is the medium of flow of energy and material cycle in the ecosystem, is tellurian precious resources, is one of necessary element of biological growth existence.
At present, China's water environment pollution problem is still very severe, and wherein, ammonia nitrogen problem is particularly outstanding, and ammonia nitrogen is one of important indicator controlling waste water objectionable impurities, is the main arch-criminal causing body eutrophication.For ammonia nitrogen concentration, bioremediation has the advantages such as persistence, high efficiency, non-secondary pollution because of it become mainstream technology means.Nitrococcus is a kind of aerobic autotrophic bacterium of obligate metabolism ammonia nitrogen, can be efficient, ammonia nitrogen in long-term metabolic waste water, it is the major microorganisms of sewage ammonia nitrogen biological treatment, but, because this ammonia nitrogen degradation microbial inoculum is living microorganism microbial inoculum, therefore not easily preserve for a long time, viable bacteria is dead very fast, and in ammonia nitrogen metabolic effects and microbial inoculum living bacteria count amount and metabolic activity state closely related.Therefore, it is a current nitrococcus Application Areas technical barrier urgently to be resolved hurrily that the long-time high vigor of viable bacteria in microbial inoculum of how effectively ensureing is preserved.
Existing microorganism live bacteria Techniques of preserving mostly is lyophilize Techniques of preserving, adds the drying and granulating preservations such as starch, adds dimethyl sulfoxide (DMSO) preservation and add citrus peel extract etc. in microbial inoculum in microbial inoculum.Dimethyl sulfoxide (DMSO) has certain bio-toxicity, the problem such as microorganism mortality, active reduction can be caused, and carrier drying technology is larger to the injury of nitrococcus activity, dried nitrosification microbial inoculum is difficult to recover metabolic activity, Freeze Drying Technique is a method of effectively preserving nitrococcus viable bacteria, but the method is relatively loaded down with trivial details, requires high to technical equipment, turnout is little, is generally only limitted to preserve bacterial classification in laboratory or industrial production and uses.The shelf time of microbiobacterial agent and preservation condition directly affect his effect, become the technical bottleneck that it is applied.At present, rarely have relevant report for the method for how effectively to preserve nitrococcus active bacteria formulation is domestic, in sum, in the production application of nitrosification microbial inoculum, still lack the feasible long-acting Techniques of preserving of efficient economy and method at present.
Summary of the invention
The object of the invention is for survival rate in the preservation of ammonia nitrogen degradation bacterium active bacteria formulation low, living contaminants is serious, the problems such as metabolic activity reduction, preserve that efficiency is high, adaptability good for ammonia nitrogen degradation microbial inoculum provides a kind of, cryopreservation preservatives with low cost and store method, to ensure that it has higher viable count and stronger metabolic activity when applying.Wherein, the microbial inoculum of ammonia nitrogen degradation described in the present invention refers to nitrococcus.
For achieving the above object; one aspect of the present invention provides a kind of ammonia nitrogen degradation microbial inoculum preservatives; by water; inhibitor, antioxidant, cytoprotective and inorganic salt conditioning agent composition; wherein; described inhibitor is nitrite, and cytoprotective is glycerine, and inorganic salt conditioning agent is dihydrogen phosphate and phosphoric acid hydrogen disalt.
Alternatively, according to ammonia nitrogen degradation microbial inoculum preservatives of the present invention, described antioxidant is D-xitix.
Alternatively, according to ammonia nitrogen degradation microbial inoculum preservatives of the present invention, containing nitrite 0.08-0.23g, D-araboascorbic acid 0.05-0.15g, glycerine 4-12mL, dihydrogen phosphate 0.13-0.38g in every 1L preservatives, phosphoric acid hydrogen disalt 2-6g, surplus is water.
Alternatively, according to ammonia nitrogen degradation microbial inoculum preservatives of the present invention, described nitrite is Sodium Nitrite.
Alternatively, according to ammonia nitrogen degradation microbial inoculum preservatives of the present invention, described dihydrogen phosphate is SODIUM PHOSPHATE, MONOBASIC, and described phosphoric acid hydrogen disalt is dipotassium hydrogen phosphate.
Alternatively, according to ammonia nitrogen degradation microbial inoculum preservatives of the present invention, containing Sodium Nitrite 0.15g, D-araboascorbic acid 0.1g in every 1L preservatives, glycerine 8mL, SODIUM PHOSPHATE, MONOBASIC 0.25g, dipotassium hydrogen phosphate 4.10g, surplus is water.
The present invention additionally provides a kind of store method of ammonia nitrogen degradation microbial inoculum on the other hand, and this store method adopts above-mentioned preservatives to preserve ammonia nitrogen degradation microbial inoculum.
Alternatively, according to store method of the present invention, described method comprises step: be resuspended in described ammonia nitrogen degradation microbial inoculum preservatives by ammonia nitrogen degradation microbial inoculum cell, be then placed in 0-4 DEG C of cryopreservation.
Other store methods in hinge structure, outstanding advantage of the present invention is:
1. each Component Source of ammonia nitrogen degradation microbial inoculum preservatives is extensive, low price, and preserving type relative ease, easily operates, the advantages such as non-secondary pollution;
2., during ammonia nitrogen degradation microbial inoculum is preserved, effectively can prevent microbiological contamination, and can not damage nitrococcus living bacterial cells, after microbial inoculum is preserved, metabolic activity is comparatively stable, and can meet the needs that microbial inoculum is applied to nitrification function strengthening in Sewage treatment systems, using value is high.
Embodiment
Below in conjunction with concrete embodiment, the invention will be further described.
Ammonia nitrogen degradation microbial inoculum used is in the following embodiments autonomous fermentative production and spreads cultivation concentrated forming.
On the one hand, the invention provides a kind of ammonia nitrogen degradation microbial inoculum preservatives, it is by water, inhibitor; antioxidant, cytoprotective and inorganic salt conditioning agent composition, wherein; described inhibitor is nitrite, and cytoprotective is glycerine, and inorganic salt conditioning agent is dihydrogen phosphate and phosphoric acid hydrogen disalt.In described preservatives; the ammonia nitrogen degradation microbial inoculum of fermentation culture is by adding antioxidant protection cell from oxidative damage; living contaminants is prevented by adding inhibitor; and inhibitor is originally as nitrococcus meta-bolites; less on nitrococcus impact; inorganic salt conditioning agent has adjustment osmotic pressure and pH cushions two functions, and for nitrococcus provides suitable Conservation environment in preservation process, protective material can effectively protect nitrococcus cell not to be damaged.By the acting in conjunction of said components, thus realize effective preservation of nitrococcus, ensure its metabolic activity.
Preferably, the antioxidant used in described ammonia nitrogen degradation microbial inoculum preservatives is D-xitix.By above-mentioned nitrite, D-xitix, dihydrogen phosphate and phosphoric acid hydrogen disalt is soluble in water respectively makes the aqueous solution, by each aqueous solution, then adds glycerine, then adds water and be supplemented to target volume and namely can be made into ammonia nitrogen degradation bacterium agent preservatives.Particularly, containing nitrite 0.08-0.23g, D-araboascorbic acid 0.05-0.15g, glycerine 4-12mL, dihydrogen phosphate 0.13-0.38g in every 1L preservatives, phosphoric acid hydrogen disalt 2-6g, surplus is with water polishing.Be understandable that, other antioxidant is also applicable to the present invention.
Further preferably, described nitrite is Sodium Nitrite, and described dihydrogen phosphate is SODIUM PHOSPHATE, MONOBASIC, and described phosphoric acid hydrogen disalt is dipotassium hydrogen phosphate.Now, containing Sodium Nitrite 0.15g, D-araboascorbic acid 0.1g in every 1L preservatives, glycerine 8mL, SODIUM PHOSPHATE, MONOBASIC 0.25g, dipotassium hydrogen phosphate 4.10g, surplus is water.
On the other hand, the invention provides a kind of method utilizing above-mentioned ammonia nitrogen degradation microbial inoculum to preserve ammonia nitrogen degradation microbial inoculum.Said method comprising the steps of: ammonia nitrogen degradation microbial inoculum cell is resuspended in described ammonia nitrogen degradation microbial inoculum preservatives, is then placed in 0-4 DEG C of cryopreservation.Usually, in this preservatives, the cell concn of ammonia nitrogen degradation microbial inoculum is less than 10
15cfu/mL is comparatively suitable, at utmost can ensure the activity of preserving rear microbial inoculum.
More according to the optional element of the ammonia nitrogen degradation microbial inoculum preservatives composition of the present invention's proposition, can design various embodiments, therefore specific embodiment is only as the exemplary illustration of specific implementation of the present invention, and does not form limitation of the scope of the invention.In order to concrete description the present invention, following examples are selected to carry out exemplary illustration.
Embodiment 1
In the present embodiment, consisting of of ammonia nitrogen degradation microbial inoculum preservatives: nitrite 0.08g, D-araboascorbic acid 0.15g, glycerine 4mL, dihydrogen phosphate 0.38g, phosphoric acid hydrogen disalt 2g and suitable quantity of water.
Make the aqueous solution by soluble in water respectively for above-mentioned material except glycerine, by each aqueous solution, then add glycerine, then make up water is to 1L, be i.e. obtained ammonia nitrogen degradation microbial inoculum preservatives.
Embodiment 2
In the present embodiment, consisting of of ammonia nitrogen degradation microbial inoculum preservatives: Sodium Nitrite 0.15g, D-araboascorbic acid 0.1g, glycerine 8mL, SODIUM PHOSPHATE, MONOBASIC 0.25g, dipotassium hydrogen phosphate 4.1g and suitable quantity of water.
Make the aqueous solution by soluble in water respectively for above-mentioned material except glycerine, by each aqueous solution, then add glycerine, make up water, to 1L, namely obtains ammonia nitrogen degradation microbial inoculum preservatives.
Embodiment 3
In the present embodiment, consisting of of ammonia nitrogen degradation microbial inoculum preservatives: nitrite 0.23g, D-araboascorbic acid 0.05g, glycerine 12mL, dihydrogen phosphate 0.13g, phosphoric acid hydrogen disalt 6g and suitable quantity of water.
Make the aqueous solution by soluble in water respectively for above-mentioned material except glycerine, by each aqueous solution, then add glycerine, make up water, to 1L, namely obtains ammonia nitrogen degradation microbial inoculum preservatives.
Embodiment 4
In the present embodiment, consisting of of ammonia nitrogen degradation microbial inoculum preservatives: Sodium Nitrite 0.08g, D-araboascorbic acid 0.05g, glycerine 12mL, SODIUM PHOSPHATE, MONOBASIC 0.13g, dipotassium hydrogen phosphate 2g and suitable quantity of water.
Make the aqueous solution by soluble in water respectively for above-mentioned material except glycerine, by each aqueous solution, then add glycerine, make up water, to 1L, namely obtains ammonia nitrogen degradation microbial inoculum preservatives.
Embodiment 5
In the present embodiment, consisting of of ammonia nitrogen degradation microbial inoculum preservatives: nitrite 0.23g, D-araboascorbic acid 0.15g, glycerine 4mL, dihydrogen phosphate 0.38g, phosphoric acid hydrogen disalt 6g and suitable quantity of water.
Make the aqueous solution by soluble in water respectively for above-mentioned material except glycerine, by each aqueous solution, then add glycerine, make up water, to 1L, namely obtains ammonia nitrogen degradation microbial inoculum preservatives.
Store method of the present invention is simple to operate, and the preservatives of use is cheap, thus greatly reduces the cost of microbial inoculum preservation and improve preservation effect.In order to the preservation effect of ammonia nitrogen degradation microbial inoculum preservatives of the present invention being described better and preserving the biological activity of rear microbial inoculum, applicant carried out following test case 1-3.
Test case 1 nitrosification microbial inoculum cryopreservative preservation effect
Nitrosification microbial inoculum after fermentation spreads cultivation is after concentrated, resuspended with the preservatives dilution of different components and proportioning respectively, after abundant mixing, be placed in 4 DEG C of low ambient temperatures to preserve, between preservation period, by MPN method (Maximum probable number method), live bacterial count is carried out to microbial inoculum, and investigate microbial inoculum metabolic activity situation and microbiological contamination situation in waste water.
(1) nitrosification microbial inoculum is preserved
No. 1 as blank group, by deionized water as preservatives, press component proportion shown in table 1 respectively for No. 2-5, add deionized water and make 25L preservatives according to the preparation process of above-mentioned preservatives, nitrococcus dilution agent after concentrated is resuspended in the closed bucket of the 25L volume that 1-5 preservatives is housed, then Cool Room 4 DEG C is placed in, cryopreservation.
Table 1 nitrosification microbial inoculum preservatives forms
(2) viable count changing conditions after preserving
Measure preservation six months front and back viable counts by MPN method respectively, changing conditions is in table 2.Result shows, the nitrosification microbial inoculum preservation effect not adding preservatives of the present invention preservation is poor, microbial inoculum is black, and with dense peculiar smell, comparatively thickness when toppling over, and that the apparent state of No. 4 No. 5 also has in various degree is rotten, though No. 3 apparent in good condition, but viable count is less after preserving, in sum, No. 2 formula preservation effects are best.
Table 2 viable count changing conditions
(3) after preserving, nitrosification microbial inoculum activity is demarcated
Nitrosification microbial inoculum before and after preserving six months carries out activity and demarcates, concrete implementation step is as follows: get nitrosification microbial inoculum 50mL and be placed in 500mL Erlenmeyer flask, add 150mL metabolic activity and demarcate substratum, 30 DEG C of shaking culture, ammonia nitrogen concentration in every 2h sampling and measuring substratum, according to ammonia nitrogen concentration change matching ammonia nitrogen metabolic chart, by curvilinear equation known nitrosification microbial inoculum ammonia nitrogen metabolic activity.Concrete metabolic activity change is in table 3.Analyze known, through the preservation of six months, No. 2 formula protective materials can better preserve the metabolic activity of nitrosification microbial inoculum.
Table 3 metabolic activity changing conditions
Wherein, the metabolic activity used demarcates substratum: (NH
4)
2sO
41.55g, KH
2pO
40.2g, MgSO
47H
2o0.04g, CaCl
20.02g, pH7.5-7.8.
(4) microbiological contamination situation changes
Carry out microscopy to the nitrosification microbial inoculum preserving front and back respectively, observe microbiological contamination situation, concrete outcome is as shown in table 4 below.Analyze known, No. 2 formula preservatives preservation effects are better than other a few group of formula, after the preservation of six months, do not produce microbiological contamination situation.
Table 4 microbiological contamination situation changes
Above-mentioned experimental result display, ammonia nitrogen degradation microbial inoculum preservatives of the present invention effectively can preserve number of viable, maintenance metabolism is active, and effectively can prevent living contaminants.
Nitrosification microbial inoculum after test case 2 preservation is to the treatment effect of municipal wastewater
Handling object is the pending waste water taking from Tianjin Xianyang road sewage work, and operation scheme is Air Exposure in 10L bucket.Concrete implementation step is as follows: numbering in table 12 preservatives is preserved the nitrosification microbial inoculum after 6 months and add in the pending waste water of 10L in the ratio that adds of 1000:1, adjustment pH is 7.4-7.8, under room temperature (20 DEG C-25 DEG C), aeration runs 1d, centrifuging and taking supernatant, measures ammonia nitrogen according to " the mensuration Berthelot spectrophotometry of Water quality ammonia nitrogen ".Result shows, and ammonia nitrogen removal frank is 95.6%.
Nitrosification microbial inoculum after embodiment 3 preservation is to the treatment effect of industrial sewage
Handling object is the pending waste water of chemical plant, Shandong sewage works, concrete implementation step is as follows: in this waste water processing station biochemistry pool, add numbering 2 preservatives in table 1 and preserve the strengthening of the nitrosification microbial inoculum after 6 months NH_3-N treating, it is 3000:1 that microbial inoculum adds ratio, treatment process is general method of exposing to the sun, water temperature: 19 DEG C-21 DEG C, influent ammonium concentration: 150mg/L, hydraulic detention time: 1d, get secondary clarifier effluent, measure ammonia nitrogen according to " the mensuration Berthelot spectrophotometry of Water quality ammonia nitrogen ", ammonia nitrogen removal frank reaches 96.7%.
It should be noted, the present invention will be described instead of limit the invention for above-described embodiment, and those skilled in the art can design alternative embodiment when not departing from the scope of claims.In the claims, any reference symbol between bracket should be configured to limitations on claims.Word " comprises " not to be got rid of existence and does not arrange element in the claims or step.Word first, second and third-class use do not represent any order, can be title by these word explanations.
Claims (8)
1. an ammonia nitrogen degradation microbial inoculum preservatives, by water, inhibitor, antioxidant, cytoprotective and inorganic salt conditioning agent composition, wherein, described inhibitor is nitrite, and cytoprotective is glycerine, and inorganic salt conditioning agent is dihydrogen phosphate and phosphoric acid hydrogen disalt.
2. ammonia nitrogen degradation microbial inoculum preservatives according to claim 1, wherein, described antioxidant is D-xitix.
3. ammonia nitrogen degradation microbial inoculum preservatives according to claim 2, wherein, containing nitrite 0.08-0.23g in every 1L preservatives, D-araboascorbic acid 0.05-0.15g, glycerine 4-12mL, dihydrogen phosphate 0.13-0.38g, phosphoric acid hydrogen disalt 2-6g, surplus is water.
4. ammonia nitrogen degradation microbial inoculum preservatives according to claim 3, wherein, described nitrite is Sodium Nitrite.
5. ammonia nitrogen degradation microbial inoculum preservatives according to claim 4, wherein, described dihydrogen phosphate is SODIUM PHOSPHATE, MONOBASIC, and described phosphoric acid hydrogen disalt is dipotassium hydrogen phosphate.
6. ammonia nitrogen degradation microbial inoculum preservatives according to claim 5, wherein, containing Sodium Nitrite 0.15g, D-araboascorbic acid 0.1g in every 1L preservatives, glycerine 8mL, SODIUM PHOSPHATE, MONOBASIC 0.25g, dipotassium hydrogen phosphate 4.10g, surplus is water.
7. a store method for ammonia nitrogen degradation microbial inoculum, wherein, this store method adopts the preservatives according to any one of claim 1-6 to preserve ammonia nitrogen degradation microbial inoculum.
8. store method according to claim 7, wherein, described method comprises step: be resuspended in described ammonia nitrogen degradation microbial inoculum preservatives by ammonia nitrogen degradation microbial inoculum cell, be then placed in 0-4 DEG C of cryopreservation.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165495A (en) * | 2018-03-22 | 2018-06-15 | 杭州微球科技有限公司 | A kind of stabilizer stored refrigerated for microorganism fungus kind |
| CN109207368A (en) * | 2018-10-31 | 2019-01-15 | 中冶华天工程技术有限公司 | Nitrify the store method of microbial inoculum |
| CN115141767A (en) * | 2022-06-15 | 2022-10-04 | 甘肃农业大学 | Special microbial agent for broccoli and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921708A (en) * | 2010-08-04 | 2010-12-22 | 浙江商达水务有限公司 | High-density culture method of mixed denitrification microorganism flora |
| CN102070250A (en) * | 2009-11-20 | 2011-05-25 | 林剑辉 | Photosynthetic bacterium water quality purifying agent and preparation method thereof |
| CN104560821A (en) * | 2014-12-28 | 2015-04-29 | 天津凯英科技发展有限公司 | Nitrobacter winogradsky XY-01, as well as preparation method and application thereof |
-
2015
- 2015-12-22 CN CN201510967936.6A patent/CN105543093B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102070250A (en) * | 2009-11-20 | 2011-05-25 | 林剑辉 | Photosynthetic bacterium water quality purifying agent and preparation method thereof |
| CN101921708A (en) * | 2010-08-04 | 2010-12-22 | 浙江商达水务有限公司 | High-density culture method of mixed denitrification microorganism flora |
| CN104560821A (en) * | 2014-12-28 | 2015-04-29 | 天津凯英科技发展有限公司 | Nitrobacter winogradsky XY-01, as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 姚秀清 等: ""水体硝化菌剂保存方法的研究"", 《化学与生物工程》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165495A (en) * | 2018-03-22 | 2018-06-15 | 杭州微球科技有限公司 | A kind of stabilizer stored refrigerated for microorganism fungus kind |
| CN108165495B (en) * | 2018-03-22 | 2021-01-12 | 杭州微球科技有限公司 | Stabilizer for refrigerating and preserving microbial strains |
| CN109207368A (en) * | 2018-10-31 | 2019-01-15 | 中冶华天工程技术有限公司 | Nitrify the store method of microbial inoculum |
| CN115141767A (en) * | 2022-06-15 | 2022-10-04 | 甘肃农业大学 | Special microbial agent for broccoli and preparation method thereof |
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