CN105524998B - A kind of and chain molecular labeling of cotton Island Cotton Fiber intensity - Google Patents
A kind of and chain molecular labeling of cotton Island Cotton Fiber intensity Download PDFInfo
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Abstract
The invention discloses a kind of and cotton fiber strength QTL/ major gene loci qFS-C12-1 and qFS-C24-1 chain molecular labelings, and qFS-C12-1 and qFS-C24-1 are located on chromosome C12 and C24.Molecular labeling with qFS-C12-1 close linkage is HAU1361240, it is PGML03854 with qFS-C24-1 chain molecular labeling240.Using the present invention, these, which carry out assisted Selection with the chain SSR marker of cotton fiber strength QTL/ major gene loci, can be improved cotton fiber strength breeding efficiency.
Description
Technical field
The present invention relates to Molecular breeding in upland cotton technical fields, and in particular to chain with cotton Island Cotton Fiber intensity to one kind
Molecular labeling and its application.
Background technique
Cotton is industrial crops important in the world, and cotton fiber is important textile industry raw material, and cotton is in China its people
Tool critical role is accounted in economy.With the fast development of textile industry and the continuous improvement of living standards of the people, to cotton fiber
The requirement of quality is also higher and higher.The breeding of fiber quality has become one of the main target of Cotton in China breeding, and fiber is strong
Degree is an important indicator of fiber quality characteristics.
Island Cotton Fiber has the excellent characteristics such as long, strong, thin, but bad adaptability, low output, and Upland Cotton Yield is high, fits
Ying Xingguang, but poor quality (Percy et al., 2006).Therefore the excellent fiber quality gene for excavating sea island cotton, sea island cotton
Excellent fiber quality gene transfer has great significance to the raising of China's upland cotton fiber quality into upland cotton background.
The fiber quality characteristics of cotton belong to the quantitative character of controlled by multiple genes.Majority fibers quality trait and yield traits
Between exist significant or not significant negatively correlated (Pan Jiaju, etc. 1998), brought to improve synchronous with yield of cotton fiber quality
Difficulty.Using traditional breeding method, the fiber quality selection of each generation all needs to roll until collecting cotton after cotton boll blowing
It could be determined after carrying out fiber quality detection after spending, and fiber quality characteristics are affected by environment larger, so that Phenotypic Selection is quasi-
True property is poor, the period is long, low efficiency, and Advances in Breeding is slow.
The development of molecular biology and quantitative character gene locus therefor drawing method has provided for cotton fibre quality improvement
Effect means.It is directly selected by genotype of the molecular labeling to objective trait, without considering plant growth period and hair
Condition is educated, can be selected in early days, and can be reduced the non-equipotential base from same site not iso-allele or different loci
Interfering with each other because between is conducive to quickly build collection target gene, accelerates back cross breeding process, overcome the unfavorable linkage of characters, greatly
Ground shortens breeding time.In terms of the gene excavating of Island Cotton Fiber quality trait, using different land-sea hybrid populations into
The building of row linkage map and the QTL of important fiber quality characteristics screen (such as Jiang et al.1998；Yu et al.1998；
Kohel et al.2001；Paterson et al.2003；Wu offer it is clear etc., 2003；Mei et al.2004；Lin et
al.2005；Yang Xinlei etc., 2009,2015；He et al.2007；Lacape et al.2005,2010；Yu et
Al.2013), very big progress is achieved.These results of study have been laid good for the molecule marking research of fiber quality characteristics
Good basis, but QTLs or molecular labeling chain therewith be applied to it is very few in molecular marker assisted selection breeding.
Majority is to utilize segregating population such as F in the research of forefathers2、BC1, genetic background is complicated, or only examines under single environment
Measuring as a result, therefore lacking reliability and stability, multi-environment stable QTL is few, and some study initial purpose only
It is the positioning in order to carry out target gene, does not account for combining with breeding material in the selection of experimental material, is also difficult to answer
It uses in breeding.Chromosome segment substitution line contains only the chromosome segment of a small number of donor parents, most of heredity in genome
Background is consistent with receptor parent, reduces the interference of genetic background, and QTL detection is more acurrate.The present invention utilizes land-sea Introgressed line
(BC4F3、BC4F3:4、BC4F3:5、BC4F3:6) filter out the molecular labeling of stable fibre strength QTLs and its close linkage, and benefit
Go out the strain that fibre strength is improved with the molecular marker screening with these QTL close linkages.
Summary of the invention
The technical problems to be solved by the present invention are: in order to overcome in traditional breeding method poor Phenotypic Selection accuracy, low efficiency,
Period is long, many disadvantages such as at high cost, solves the problems, such as that fiber quality breedin is made slow progress, provides a kind of from Materials with High Strength sea
Island cotton sea 1 and the chain molecular labeling of cotton fiber strength QTL/ major gene resistance, it may be assumed that Materials with High Strength sea island cotton is come from by screening
The SSR molecular marker chain with fibre strength QTL/ major gene resistance in sea 1, carries out the early stage marker assisted selection on DNA level,
Improve breeding efficiency.
Present invention provide the technical scheme that wherein, molecular labeling HAU1361240Specific primer HAU1361 its just
To sequence as shown in SEQ ID NO.1, reverse sequence is as shown in SEQ ID NO.2, the specific mark gone out with the primer amplification, item
Band molecular weight is 240bp；Molecular labeling PGML03854240Specific primer PGML03854 its positive sequence such as SEQ ID
Shown in NO.3, reverse sequence is as shown in SEQ ID NO.4, the specific mark gone out with the primer amplification, and band molecular weight is
The specific primer sequence of each molecular labeling of 240bp, C12 and the target fragment length of amplification are as follows:
The present invention also provides a kind of upland cotton fiber intensity auxiliary breeding means and utilize above-mentioned and cotton fiber strength
The chain molecular labeling of QTL/ major gene loci is in cotton assistant breeding to improve the application in cotton fiber strength.
A kind of upland cotton fiber intensity auxiliary breeding means, this method comprises the following steps:
(1) seedling stage single plant extracts DNA；
(2) the molecule mark chain with cotton fiber strength QTL/ major gene loci qFS-C12-1 and qFS-C24-1 is used
Note, respectively HAU1361240And PGML03854240, Molecular Detection is carried out to the genotype of group's single plant, and with cotton in upland cotton
Institute 45 and extra large 1 genotype of sea island cotton are as control；
(3) testing result is analyzed；
(4) plant of the selection with extra large 1 characteristic bands of sea island cotton obtains the single plant that fibre strength is improved.
According to the present invention and upland cotton fiber intensity auxiliary breeding means, use SSR marker HAU1361240With
PGML03854240Marker-assisted selection is carried out to fibre strength character in breeding population related with sea island cotton sea 1 etc., can be mentioned
The fibre strength of high upland cotton.Molecular labeling HAU1361 used in this method240And PGML03854240It is strong with cotton fiber respectively
(FS is the contracting of the English word fiber strength of fibre strength to 2 QTLs:qFS-C12-1 and qFS-C24-1 of degree character
It writes；The name of QTL: the sequence that character QTL is controlled on q+ character title english abbreviation+chromosome serial number+same chromosome is compiled
Number.Such as: qFS-C12-1 indicates the 1st QTL that fibre strength is controlled on the 12nd article of chromosome) close linkage.This 2 fibers
The QTLs:qFS-C12-1 and qFS-C24-1 of strength behavior are located on chromosome C12 and C24, all from sea island cotton sea
1, the contribution rate to cotton fiber strength is respectively 4.94-12.61% and 3.63-8.92%, and additive effect is respectively 0.47-
1.08cN/tex and 0.58-1.04cN/tex.
The present invention not only facilitates screening high fiber strength strains, for 1 hybridization of the sea of sea island cotton from now on, backcross progeny and its derivative
The fibre strength character breeding utilization of strain provides a great convenience, while also establishing for the finely positioning of QTL and gene cloning
Fixed basis.It can get the Upland Cotton or strain that fibre strength is improved by these Marker-assisted selections, accelerate cotton
The breeding process of fiber quality.
The height of fibre strength can be predicted in cotton in seedling stage using the present invention and carry out superseded, and then can quickly screen
The high strain of fibre strength is used for cotton fiber quality breeding, improves the accuracy and efficiency of selection.
Point chain with cotton fiber strength QTL/ major gene loci qFS-C12-1 and qFS-C24-1 of the present invention
Son label is to obtain by the following method according to the following steps:
(1) it is donor parents with sea island cotton sea 1, is receptor parent with nakamise 45, by hybridizing high generation backcrossing, continuously certainly
It hands over, constructs the chromosome segment substitution line that a set of nakamise 45 is genetic background, totally 116 BC4F1Family (Yang Zemao etc.,
2009).By selfing in 2 years, 2328 BC of acquisition in 20094F3Single plant.Per stirpes select 332 BC at random4F3Single plant 2010
Year, kind was at BC4F3:4Plant mixes and receives BC4F3:4Plant seed, kind in 2011 at two environment BC4F3:5Strain (Anyang and Ku Er
Strangle), it mixes and receives BC4F3:5Strain seed, kind in 2014 at three environment BC4F3:6Strain (Zhoukou City, Kuerle and Aksu).It is right
Each each environment of generation population carries out the investigation and fiber quality measurement of Agronomic characteristic.
(2) DNA is extracted to 332 substitution lines in (1) and its parent, according to the method for (Paterson et al.1993)
Extract DNA.
(3) from the present inventor with nakamise 36 × sea 1BC1F1High Density Molecular genetic linkage maps (the Shi of informative population
Et al.2015) on, a SSR marker is selected every about 10cM, filters out the 520 of whole 26 chromosomes of covering cotton altogether
A marker site, and with it to 332 chromosome segment replacement based materials and its parent's nakamise 36 and sea 1 in above-mentioned (2)
DNA carries out genotype detection.
(4) seven environment (Anyang in 2009, Anyang in 2010, Anyang in 2011, Kuerles in 2011,2014 are utilized
Zhoukou City, Kuerles in 2014 and Aksu in 2014) fibre strength character phenotypic data and genotype data, using QTL
IciMapping V4.0 software (http://www.isbreeding.net/software/) carries out the more of fibre strength character
Environment QTL positioning analysis obtains multi-environment stable fibre strength character QTLs, wherein 2 QTLs can in five environment table
It is now stable, be it is newfound, this 2 QTL be qFS-C12-1 and qFS-C24-1, be located on chromosome C12 and C24, increase
Effect gene all derives from sea island cotton sea 1, and the contribution rate to cotton fiber strength is respectively 4.94-12.61% and 3.63-
8.92%, additive effect is respectively 0.47-1.08cN/tex and 0.58-1.04cN/tex；With this 2 fibre strength characters
The SSR molecular marker of QTLs close linkage is respectively HAU1361240And PGML03854240。
Beneficial effects of the present invention are as follows:
The present invention provides a kind of method for selecting molecular marker for improving upland cotton fiber strength behavior, use molecular labeling
HAU1361240And PGML03854240With carry out Marker-assisted selection in the extra large 1 related breeding population of sea island cotton, fibre can be improved
Tie up intensity 0.47-1.08cN/tex and 0.58-1.04cN/tex.
It can be selected in cotton in seedling stage using the molecular labeling in the present invention, improve the selection effect of fibre strength character
Rate.The present invention, which not only helps, solves the problems, such as that Cotton in China fiber quality breedin is made slow progress, and helps to overcome existing
Breeding technique identifies the deficiencies of existing time is long, accuracy is poor, low efficiency to fiber quality, rapidly improves existing upland cotton
The fiber quality of kind greatly speeds up the cultivation process of the high good fiber quality new varieties in China.
Detailed description of the invention
Fig. 1 is the present invention 2 QTLs related with fibre strength in the position of molecular marker linkage maps.
Wherein, qFS-C12-1 and qFS-C24-1 is located on chromosome C12 and C24.
FS is the abbreviation of the English word fiber strength of fibre strength.
The name of QTL: the suitable of character QTL is controlled on q+ character title english abbreviation+chromosome serial number+same chromosome
Sequence number.Such as: qFS-C12-1 indicates the 1st QTL that fibre strength is controlled on the 12nd article of chromosome.
Specific embodiment
Come that the present invention is furture elucidated below by the detailed description of specific embodiment mode, but is not to of the invention
Limitation, only illustrates.
Embodiment 1 screens molecular labeling
(1) building of chromosome segment substitution line and the acquisition of phenotypic data
The present inventor with sea island cotton sea 1 is donor parents, with nakamise 45 (commercial variety) for receptor parent, pass through hybridization
High generation backcrossing, it is continuous to be selfed, the chromosome segment substitution line that a set of nakamise 45 is genetic background is constructed, is obtained within 2007
116 BC4F1Family (Yang Zemao etc., 2009).Nakamise 45 (state examines cotton 2003002) is the research of Chinese Academy of Agricultural Sciences cotton
The medium variety with high yield verticillium wilt characteristic resistant to lodging, resistance to cultivated, and donor parents sea 1 (Hai1) have it is excellent
Fiber quality and the sea island cotton cultivar for having dominant non-gland gene.
116 BC are planted in Anyang within 20084F2Family, each 1 row of family kind, every 20 strains plant 1 row nakamise 45
As control, row long 5m, spacing in the rows 25cm, per stirpes are mixed to harvest to obtain BC4F3.116 BC of plantation in 20094F3Family (each family
About 20 plants of 1 row, totally 2328 plants), nature bell is collected by single plant, measures fiber quality, fiber-like send the Ministry of Agriculture's fiber quality
Inspection and supervision test center is with the upper half mean length of HVI1000 measurement fiber, uniformity, mic value, elongation and breaks
Split specific strength (abbreviation intensity).Obtain 2328 BC4F3Individual fiber qualitative data.According to each families selecting 2-4 plants of original
Then, 332 BC are selected altogether4F3Single plant, 2010 in Earthquake of Anyang station in Henan kind at BC4F3:4Plant, capable long 5m, line width 80cm, spacing in the rows
25cm, according to mixed sowing of row.Two environment (Anyang and Kuerle) of setting in 2011 are tested, and are planted into BC4F3:5Strain, field
Between plant and sowed by the way of covering with ground sheeting, the double multiple cropping in Anyang uniline area is planted, row long 5m, line width 80cm, spacing in the rows 25cm, newly
The double multiple cropping in 2 row area of boundary is planted, and the long 3m of row, line-spacing is arranged by Xinjiang locality wide-and narrow-row, and spacing in the rows 10cm mixes sowing according to cell
Son.
Three environment (Zhoukou City, Kuerle and Aksu) of setting in 2014 are tested, and are planted into BC4F3:6Strain, Zhoukou City's uniline
The double multiple cropping in area is planted, and row long 5m, line width 100cm, spacing in the rows 30cm, the double multiple cropping in Xinjiang Kuerle and 2 row area of Aksu are planted, row length
3m, line-spacing are arranged by Xinjiang locality wide-and narrow-row, spacing in the rows 10cm.Fiber quality, 2010-2011 are measured according to single plant within 2009
With 2014, according to cell measurement fiber quality.Obtain the data (table 1) of the fiber quality of seven environment.
Chromosome segment substitution line fibre strength statistical analysis and 45 fibre strength of nakamise are averaged in 1 seven environment of table
Value performance
AY: the Anyang in Henan；KEL: the Kuerle in Xinjiang；ZK: the Zhoukou City in Henan；AKS: the Aksu in Xinjiang
(2) DNA is extracted
332 chromosome segment substitution line DNA and parent dna are extracted according to the method for (Paterson et al.1993).
(3) genotype detection
From the present inventor with nakamise 36 × sea 1BC1F1High Density Molecular genetic linkage maps (the Shi et of informative population
Al.2015 on), a SSR marker is selected every about 10cM, altogether 520 marks of whole 26 chromosomes of selection covering cotton
Remember site (table 2), and genotype detection, nakamise are carried out to above-mentioned (2) 332 chromosome segments replacement based material DNA with it
45 are used as control with extra large 1 genotype.
Primer is won company and is synthesized by the raw work in Shanghai and Beijing three.SSR amplification reaction system is 10 μ 1, wherein 10 × Buffer
0.50 μ 1 of 1.0 μ 1,10mM dNTPs, 0.50 μ 1 of forward primer (10 μM), 0.50 μ 1 of reverse primer (10 μM), template DNA
(30ng/ μ 1) 1.0 μ 1, Taq archaeal dna polymerase (5U/ μ 1) 0.10 μ 1,6.40 μ 1 of ultrapure water.SSR amplified reaction program: 94 DEG C pre-
It is denaturalized 45s；94 DEG C of denaturation 30s, 57 DEG C of annealing 45s, 72 DEG C of extension 1min, 29 recycle.94 DEG C of denaturation 60s, 57 DEG C of annealing
45s, 72 DEG C of extension 2min.Amplified reaction carries out on BIO-RAD company PTC-200 and BIOMETRA company's T GRADIENT, expands
Volume increase object carries out electrophoresis in 8% polyacrylate hydrogel, carries out gel silver staining according to the method for Zhang Jun (2000), records result.
The SSR marker that table 2 is selected from Molecular Markers Linkage Map
(4) the QTL positioning of fibre strength
It is right using QTL IciMapping V4.0 software (http://www.isbreeding.net/software/)
332 chromosome segment substitution line groups, seven environment (Anyang in 2009, Anyang in 2010, Anyang in 2011,2011 Ku Er
Le, Zhoukou City in 2014, Kuerles in 2014 and Aksu in 2014) fibre strength character phenotypic data and genotype data
QTL positioning analysis is carried out, detects multi-environment stable QTLs, 2 QTLs are newfound.This newfound 2 QTLs
Middle qFS-C12-1 can be in Anyang in 2009, Anyang in 2011, Kuerle in 2011, Zhoukou City in 2014 and Aksu in 2014
It is detected in five environment, qFS-C24-1 can be in Anyang in 2009, Kuerle in 2011, Zhoukou City in 2014, library in 2014
It is detected in Er Le and 2014 year five, Aksu environment, concrete outcome is shown in Table 3.
The QTLs for 2 fibre strengths that 3 five environment of table can detect
AY: the Anyang in Henan；KEL: the Kuerle in Xinjiang；ZK: the Zhoukou City in Henan；AKS: the Aksu in Xinjiang
The qFS-C12-1 and qFS-C24-1 of this 2 fibre strength characters are located on chromosome C12 and C24 (figure
1), additive effect is all positive, and synergy gene all derives from sea island cotton sea 1, and the contribution rate to cotton fiber strength is respectively 4.94-
12.61% and 3.63-8.92%, additive effect are respectively 0.47-1.08cN/tex and 0.58-1.04cN/tex；With this 2 fibres
The SSR molecular marker for tieing up the QTLs close linkage of strength behavior is respectively HAU1361240And PGML03854240。
Wherein, the specific primer sequence of each molecular labeling and the target fragment length of amplification are as follows:
The method for selecting molecular marker that 2 upland cotton fiber strength behavior of embodiment improves
The molecular labeling HAU1361 obtained using embodiment 1240And PGML03854240Related with sea island cotton sea 1 etc.
Marker-assisted selection is carried out in breeding population, comprising the following steps:
(1) DNA is extracted: being donor parents, Upland Cotton or strain (such as Lu Mianyan 28, nakamise with sea island cotton sea 1
60) it is receptor parent, is hybridized, is returned, obtains segregating population, or with sea island cotton sea 1 be donor parents, Upland Cotton
(such as Lu Mianyan 28, nakamise 60) is the chromosome segment substitution line and its spin-off that the high generation backcrossing of receptor parent hybridization obtains
The progeny population that system or its chromosome segment substitution line hybridize with Upland Cotton, are returned uses CTAB method in seedling stage
(Paterson et al.1993) extracts segregating population single plant DNA；
(2) molecular labeling HAU1361 is used240And PGML03854240The genotype of above-mentioned (1) group single plant is divided
Son label detection, using sea 1 and 45 genotype of nakamise as compare；
(3) testing result is analyzed；
(4) plant of the selection with extra large 1 characteristic bands of sea island cotton, fibre strength of menu strain is likely to be obtained difference in these
The raising (table 4) of degree.
The " CCRI 36 of 4 Marker-assisted selection of table and sea 1 are returned the table for being selfed the strain fibre strength in 5 generations in 5 generations
It is existing
| Material number | HAU1361240 | PGML03854240 | Average fiber intensity (cN/tex) |
| ZLN020 | A | 31.95 | |
| ZLN021 | A | 31.10 | |
| ZLN057 | A | 31.80 | |
| ZLN114 | A | 30.45 | |
| ZLN144 | A | 30.95 | |
| ZLN154 | A | 31.30 | |
| ZLN170 | A | 30.00 | |
| ZLN228 | A | 30.75 | |
| ZLN235 | A | 31.85 | |
| ZLN240 | A | 30.65 | |
| ZLN241 | A | 31.15 | |
| ZLN404 | A | 30.20 | |
| CK (nakamise 36) | 29.95 |
" A " indicates to detect the characteristic strip of label
It can get the Upland Cotton (being) that fibre strength is improved through the invention, cotton fiber quality can be accelerated
Breeding process.
<110>the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute
<120>a kind of and chain molecular labeling of cotton Island Cotton Fiber intensity
<160> 4
<210> 1
<211> 23
<212> DNA
<213>artificial sequence
<220>
<223>description of artificial sequence: artificial synthesized sequence
<400> 1
CCTGCCATGAGAGAAGTTTT 23
<210> 2
<211> 24
<212> DNA
<213>artificial sequence
<220>
<223>description of artificial sequence: artificial synthesized sequence
<400> 2
TGAAAGTTTGAATGGGAAAG 24
<210> 3
<211> 19
<212> DNA
<213>artificial sequence
<220>
<223>description of artificial sequence: artificial synthesized sequence
<400> 3
TCTTTGAAGGCCTGAGGGT 19
<210> 4
<211> 20
<212> DNA
<213>artificial sequence
<220>
<223>description of artificial sequence: artificial synthesized sequence
<400> 4
AAAGCCAAGCAAGAACATTG 20
Claims (3)
1. a kind of exist with cotton fiber strength QTL/ major gene loci qFS-C12-1 and qFS-C24-1 chain molecular labeling
Application in cotton breeding, it is characterised in that: qFS-C12-1 and qFS-C24-1 is located on chromosome C12 and C24, with
The label of qFS-C12-1 close linkage is240；Chain label is with qFS-C24-1240, wherein point
Son label HAU1361240Specific primer HAU1361 its positive sequence as shown in SEQ ID NO.1, reverse sequence such as SEQ
Shown in ID NO.2, the specific mark gone out with the primer amplification, band molecular weight is 240bp；Molecular labeling PGML03854240's
Its positive sequence of specific primer PGML03854 is as shown in SEQ ID NO.3, and reverse sequence is as shown in SEQ ID NO.4, with this
The specific mark that primer amplification goes out, band molecular weight are 240bp；
The application refers to the application improved in cotton fiber strength.
2. a kind of upland cotton fiber intensity auxiliary breeding means, which is characterized in that method includes the following steps:
(1) seedling stage single plant DNA is extracted；
(2) molecular labeling chain with cotton fiber strength QTL/ major gene loci qFS-C12-1 and qFS-C24-1 is used,
Respectively HAU1361240And PGML03854240, Molecular Detection is carried out to the genotype of group's single plant, and with " CCRI
45 and extra large 1 genotype of sea island cotton as control；
(3) testing result is analyzed；
(4) plant of the selection with extra large 1 characteristic bands of sea island cotton obtains the single plant that fibre strength is improved；
Wherein, described and cotton fiber strength QTL/ major gene loci qFS-C12-1 and qFS-C24-1 chain molecular labeling
Specific primer be respectively HAU1361 and PGML03854, HAU1361 primer forward direction sequence is as shown in SEQ ID NO.1, instead
To sequence as shown in SEQ ID NO.2, the specific mark gone out with the primer amplification, band molecular weight is 240bp；PGML03854
Its positive sequence is as shown in SEQ ID NO.3, and reverse sequence is as shown in SEQ ID NO.4, with the special mark of the primer amplification out
Note, band molecular weight are 240bp.
3. according to the method described in claim 2, it is characterized by: extracting DNA in step (1): being donor parent with sea island cotton sea 1
This, Upland Cotton or strain are receptor parent, are hybridized, are returned, and obtain segregating population, or with sea island cotton sea 1 to supply
Body parent, Upland Cotton are the chromosome segment substitution line and its derivative strain that the high generation backcrossing of receptor parent hybridization obtains, or
The progeny population that its chromosome segment substitution line of person hybridizes with Upland Cotton, is returned extracts segregating population single plant in seedling stage
DNA。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610035482.3A CN105524998B (en) | 2016-01-19 | 2016-01-19 | A kind of and chain molecular labeling of cotton Island Cotton Fiber intensity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610035482.3A CN105524998B (en) | 2016-01-19 | 2016-01-19 | A kind of and chain molecular labeling of cotton Island Cotton Fiber intensity |
Publications (2)
| Publication Number | Publication Date |
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| CN108179214B (en) * | 2017-11-29 | 2021-11-23 | 中国农业科学院棉花研究所 | Molecular breeding method for synchronously improving verticillium wilt resistance, fiber quality and yield traits of cotton |
| CN110616275B (en) * | 2019-10-25 | 2022-07-26 | 南京农业大学 | Molecular marker derived from Yttrium okamuni cotton and cotton fiber strength QTL (quantitative trait locus) linkage and application thereof |
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Non-Patent Citations (6)
| Title |
|---|
| Anonymous.HAU1361.《互联网文章》.2012,第1页. * |
| Pawan Kumar et al.Mapping and Validation of Fiber Strength Quantitative Trait Loci on Chromosome 24 in Upland Cotton.《CROP SCIENCE》.2012,第52卷第1115-1122页. * |
| Yuzhen Shi et al.Constructing a high-density linkage map for Gossypium hirsutum×Gossypium barbadense and identifying QTLs for lint percentage.《Journal of Integrative Plant Biology》.2015,第57卷(第5期),第450-467页. * |
| 何蕊等.利用染色体片段代换系定位陆地棉株高QTL.《作物学报》.2014,第40卷(第3期),第457-465页. * |
| 葛瑞华.利用陆海杂交BC_1F_1群体构建遗传图谱及定位纤维品质与产量性状的QTL.《中国优秀硕士学位论文全文数据库（电子期刊）》.2013,第D047-196页. * |
| 马麟等.利用海岛棉染色体片段置换系改良新陆早45号纤维品质性状的研究.《棉花学报》.2013,第25卷(第6期),第496-502页. * |
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