CN105519418A - Method for transplanting passiflora edulis tissue cultured seedlings - Google Patents
Method for transplanting passiflora edulis tissue cultured seedlings Download PDFInfo
- Publication number
- CN105519418A CN105519418A CN201511024327.3A CN201511024327A CN105519418A CN 105519418 A CN105519418 A CN 105519418A CN 201511024327 A CN201511024327 A CN 201511024327A CN 105519418 A CN105519418 A CN 105519418A
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- China
- Prior art keywords
- passionflower
- plantlet
- vitro
- tissue cultured
- passiflora edulis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention belongs to the technical field of fruit tree cultivation, and particularly relates to a method for transplanting passiflora edulis tissue cultured seedlings. The method comprises the following steps of (1) taking out clusters of cultivated passiflora edulis tissue cultured seedlings from a culture vessel, placing the clusters in a container having a hollow bottom and four hollow sides, and determining the number of placed clusters according to the size of the container; (2) completely soaking roots of the passiflora edulis tissue cultured seedlings in the whole container in disinfectant for 1-10 minutes, and taking out the roots from the disinfectant; and (3) planting the clusters of the passiflora edulis tissue cultured seedlings in a culture medium. The method can be used for increasing the transplanting survival rate of the passiflora edulis tissue cultured seedlings, and the tissue cultured seedlings can quickly grow and recover; the disinfectant used in the method is reasonable in formula, can be used for reducing occurrence of damping-off pathogenic bacteria in the transplanting period of the passiflora edulis tissue cultured seedlings, and is beneficial to growth of roots of the transplanted passiflora edulis tissue cultured seedlings; and the culture medium used in the method has an effect of air permeability and water retention, and is beneficial to survival and growth of the passiflora edulis tissue cultured seedlings.
Description
Technical field
The invention belongs to fruit tree cultivation technical field, specifically relate to a kind of transplanting method of passionflower plantlet in vitro.
Background technology
Passionflower (PassifloraeduliaSims), has another name called passion fruit, egg fruit, is the herbaceous species plant of Passifloraceae Passiflora, is about 6 meters; Stem tool stria, without hair; 5 pieces, petal, isometric with sepal; Base portion light green, middle part purple, top white, berry ovoid, diameter 3-4 centimetre, without hair, purple time ripe; Seed is most, avette.June at florescence, really November phase.
Vegetables, feed be eaten or be done to passionfruit can raw.Be used as medicine and there is excitement, strong effect.Fruit flesh succulence liquid, adds calcium bicarbonate and sugar, can be made into the beverage that fragrance is good to eat, also can be used to be added in Other Drinks to improve the quality of beverage.Seed extracts oil, can edible and soapmaking, liquefaction paint etc.Spend beautiful greatly, there is no fragrance, flower garden ornamental plants can be done.Other areas beyond China, egg fruit has the laudatory title such as " king of fruit juice ", " cash cow ".
Passionflower tradition adopts cutting propagation, and the seedling of breeding has trait depression, carries the shortcoming of disease worm.Plantlet in vitro technology as a kind of emerging biotechnology just Application and Development in passionflower plantation in.Passionflower plantlet in vitro, in transplanting process, owing to being transplanted to the matrix of planting of carrying disease germs from aseptic medium, because matrix is unholiness, carries disease germs to some extent, easily causes root germ to fall ill to cause rotten, rotten seedling and reduce the transplanting survival rate of plantlet in vitro; Conventional method needs before transplanting to remove medium through washing seedling, most of root system can be caused to damage and cause plant root dysfunction, and then affect the growth of passionflower seedling; Traditional cultivation matrix generally adopts earth, its gas permeability and moisture capacity poor, be difficult to for passionflower seedling provides suitable moisture and nutrition, thus affect the growth of passionflower seedling.
The information being disclosed in this background technology part is only intended to increase the understanding to general background of the present invention, and should not be regarded as admitting or imply in any form that this information structure has been prior art that persons skilled in the art are known.
Summary of the invention
For the defect of prior art, the object of this invention is to provide a kind of transplanting method of passionflower plantlet in vitro, the inventive method is transplanted not to be needed to wash seedling operation to passionflower seedling, plantlet in vitro is dipped thimerosal, then be colonizated in and take root in matrix, passionflower plantlet in vitro transplanting method of the present invention can improve the survival rate of passionflower plantlet in vitro.
The present invention is achieved through the following technical solutions:
A transplanting method for passionflower plantlet in vitro, comprises the following steps:
(1) take out in the passionflower plantlet in vitro Cheng Congcong culture vessel cultivated, be placed in the container of bottom and surrounding hollow out, determine the quantity of putting according to container size;
(2) immersed completely in thimerosal by the root of the passionflower plantlet in vitro in whole container, the immersion time is 1 ~ 10min, then takes out;
(3) the passionflower plantlet in vitro Cheng Cong taken out again is colonizated in cultivation matrix.
As preferably, the passionflower plantlet in vitro taken out in step (1) does not remove the medium of root.
As preferably, the thimerosal described in step (2) is by mass percentage containing following component: carbendazim 30 ~ 40%, agricultural streptomycin 20 ~ 40% and 30 ~ 40% dislike mould spirit.
As preferably, described thimerosal in use extension rate is 1000 ~ 2000 times.
As preferably, the cultivation matrix described in step (3) by volume percentage contains following component: peat soil 90%, perlite 5% and vermiculite 5%.
Compared with prior art, the present invention has following beneficial effect:
1. the transplanting method of passionflower plantlet in vitro of the present invention need not wash seedling when plantlet in vitro field planting, can saving of work and time, raises labour efficiency.
2. the transplanting method of passionflower plantlet in vitro of the present invention can improve the transplanting survival rate of passionflower plantlet in vitro, and plantlet in vitro growth recovery is fast.
3. the thimerosal formula used in the inventive method is reasonable, both can prevent the generation of passionflower plantlet in vitro nursery stage disease, can promote that again passionflower accelerates to take root.
4. the cultivation matrix used in the inventive method has effect of ventilative water conservation, is easy to surviving and growing of passionflower seedling.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in detail, but is to be understood that protection scope of the present invention not by the restriction of embodiment.
Clearly represent unless otherwise other, otherwise in whole specification and claims, term " comprise " or its conversion as " comprising " or " including " etc. by be understood to include the element of stating or part, and do not get rid of other element or other part.
embodiment 1:
A transplanting method for passionflower plantlet in vitro, comprises the following steps:
(1) take out in the passionflower plantlet in vitro Cheng Congcong culture vessel cultivated, be placed in the container of bottom and surrounding hollow out, container is permeable basket, and every basket fills 30 clumps; The passionflower plantlet in vitro taken out does not remove the medium of root;
(2) immersed completely in thimerosal by the root of the passionflower plantlet in vitro in whole container, the immersion time is 1min, then takes out; Described thimerosal is by mass percentage containing following component: carbendazim 30%, agricultural streptomycin 40% and 30% dislike mould spirit; Described thimerosal in use extension rate is 1000 times;
(3) the passionflower plantlet in vitro Cheng Cong taken out again is colonizated in cultivation matrix; Described cultivation matrix by volume percentage contains following component: peat soil 90%, perlite 5% and vermiculite 5%.
embodiment 2:
A transplanting method for passionflower plantlet in vitro, comprises the following steps:
(1) take out in the passionflower plantlet in vitro Cheng Congcong culture vessel cultivated, be placed in the container of bottom and surrounding hollow out, container is permeable basket, and every basket fills 35 clumps; The passionflower plantlet in vitro taken out does not remove the medium of root;
(2) immersed completely in thimerosal by the root of the passionflower plantlet in vitro in whole container, the immersion time is 10min, then takes out; Described thimerosal is by mass percentage containing following component: carbendazim 40%, agricultural streptomycin 20% and 40% dislike mould spirit; Described thimerosal in use extension rate is 2000 times;
(3) the passionflower plantlet in vitro Cheng Cong taken out again is colonizated in cultivation matrix; Described cultivation matrix by volume percentage contains following component: peat soil 90%, perlite 5% and vermiculite 5%.
embodiment 3:
A transplanting method for passionflower plantlet in vitro, comprises the following steps:
(1) take out in the passionflower plantlet in vitro Cheng Congcong culture vessel cultivated, be placed in the container of bottom and surrounding hollow out, container is permeable basket, and every basket fills 25 clumps; The passionflower plantlet in vitro taken out does not remove the medium of root;
(2) immersed completely in thimerosal by the root of the passionflower plantlet in vitro in whole container, the immersion time is 5min, then takes out; Described thimerosal is by mass percentage containing following component: carbendazim 35%, agricultural streptomycin 30% and 35% dislike mould spirit; Described thimerosal in use extension rate is 1500 times;
(3) the passionflower plantlet in vitro Cheng Cong taken out again is colonizated in cultivation matrix; Described cultivation matrix by volume percentage contains following component: peat soil 90%, perlite 5% and vermiculite 5%.
The passionflower plantlet in vitro of being transplanted by the method described in embodiment 1-3, control group 1-3 is that (control group 1,2,3 is respectively and adopts carbendazim, agricultural streptomycin and evil mould clever thimerosal 1000 times sterilization 30min the common transplanting method passionflower plantlet in vitro of transplanting, then transplant after adopting clear water to carry out washing seedling, contrast matrix is common soil).Measure the content of organic matter and saturation moisture capacity of transplanting cultivation matrix after passionflower plantlet in vitro by experiment, and the passionflower plantlet in vitro transplanting survival rate after transplanting and the passionflower damping off incidence of disease are investigated, added up, the results detailed in Table 1.
Table 1 uses the performance measurement after transplanting passionflower plantlet in vitro of the inventive method
As shown in Table 1, the content of organic matter in the cultivation matrix after using method of the present invention to transplant, saturation moisture capacity are all higher than contrast; The transplanting survival rate of the passionflower plantlet in vitro after using method of the present invention to transplant is far above contrast; The damping off incidence of disease of the passionflower plantlet in vitro after using method of the present invention to transplant is far below contrast.
In sum, the transplanting method of passionflower plantlet in vitro of the present invention can improve the transplanting survival rate of passionflower plantlet in vitro; The thimerosal formula that the present invention uses is reasonable, both can prevent the generation of passionflower damping off, can promote that again passionflower accelerates to take root; The cultivation matrix that the present invention uses has effect of ventilative water conservation, and be easy to surviving and growing of passionflower seedling, method of the present invention has highly application value.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.
Claims (5)
1. a transplanting method for passionflower plantlet in vitro, is characterized in that, comprises the following steps:
(1) take out in the passionflower plantlet in vitro Cheng Congcong culture vessel cultivated, be placed in the container of bottom and surrounding hollow out, determine the quantity of putting according to container size;
(2) immersed completely in thimerosal by the root of the passionflower plantlet in vitro in whole container, the immersion time is 1 ~ 10min, then takes out;
(3) the passionflower plantlet in vitro Cheng Cong taken out again is colonizated in cultivation matrix.
2. the transplanting method of passionflower plantlet in vitro according to claim 1, is characterized in that, the passionflower plantlet in vitro taken out in step (1) does not remove the medium of root.
3. the transplanting method of passionflower plantlet in vitro according to claim 1, it is characterized in that, the thimerosal described in step (2) is by mass percentage containing following component: carbendazim 30 ~ 40%, agricultural streptomycin 20 ~ 40% and 30 ~ 40% dislike mould spirit.
4. the transplanting method of passionflower plantlet in vitro according to claim 3, is characterized in that, described thimerosal in use extension rate is 1000 ~ 2000 times.
5. the transplanting method of passionflower plantlet in vitro according to claim 1, is characterized in that, the cultivation matrix described in step (3) by volume percentage contains following component: peat soil 90%, perlite 5% and vermiculite 5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511024327.3A CN105519418A (en) | 2015-12-30 | 2015-12-30 | Method for transplanting passiflora edulis tissue cultured seedlings |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201511024327.3A CN105519418A (en) | 2015-12-30 | 2015-12-30 | Method for transplanting passiflora edulis tissue cultured seedlings |
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| CN105519418A true CN105519418A (en) | 2016-04-27 |
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| CN201511024327.3A Pending CN105519418A (en) | 2015-12-30 | 2015-12-30 | Method for transplanting passiflora edulis tissue cultured seedlings |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739341A (en) * | 2004-08-24 | 2006-03-01 | 易自力 | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum |
| CN101578960A (en) * | 2009-06-24 | 2009-11-18 | 华中农业大学 | Method for reducing aerial root of tissue culture seedling of hydrangea |
| CN103355142A (en) * | 2013-07-04 | 2013-10-23 | 中国热带农业科学院热带作物品种资源研究所 | A kind of passionflower soilless culture method |
| CN104904544A (en) * | 2015-06-02 | 2015-09-16 | 广西大学 | Segmented fruit-setting cultivation technique for passion fruit tissue culture seedlings |
-
2015
- 2015-12-30 CN CN201511024327.3A patent/CN105519418A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739341A (en) * | 2004-08-24 | 2006-03-01 | 易自力 | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum |
| CN101578960A (en) * | 2009-06-24 | 2009-11-18 | 华中农业大学 | Method for reducing aerial root of tissue culture seedling of hydrangea |
| CN103355142A (en) * | 2013-07-04 | 2013-10-23 | 中国热带农业科学院热带作物品种资源研究所 | A kind of passionflower soilless culture method |
| CN104904544A (en) * | 2015-06-02 | 2015-09-16 | 广西大学 | Segmented fruit-setting cultivation technique for passion fruit tissue culture seedlings |
Non-Patent Citations (3)
| Title |
|---|
| 杨冬业，张丽珍，徐淑庆: "《百香果组织培养及植株再生》", 《北方园艺》 * |
| 胡颂平: "《植物细胞组织培养技术》", 31 December 2014 * |
| 陈银全: "《西番莲茎段离体培养技术》", 《福建农业科技》 * |
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Application publication date: 20160427 |
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