CN105503768A - Preparation method of alpha-oxoglutarate fluorescent/ultraviolet molecular probe and application of alpha-oxoglutarate fluorescent/ultraviolet molecular probe to biological samples - Google Patents
Preparation method of alpha-oxoglutarate fluorescent/ultraviolet molecular probe and application of alpha-oxoglutarate fluorescent/ultraviolet molecular probe to biological samples Download PDFInfo
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- CN105503768A CN105503768A CN201610050322.6A CN201610050322A CN105503768A CN 105503768 A CN105503768 A CN 105503768A CN 201610050322 A CN201610050322 A CN 201610050322A CN 105503768 A CN105503768 A CN 105503768A
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- fluorescence
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- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000012472 biological sample Substances 0.000 title abstract description 12
- KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 title abstract 10
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- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 12
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- 238000004458 analytical method Methods 0.000 claims abstract description 12
- IGHBXJSNZCFXNK-UHFFFAOYSA-N 4-chloro-7-nitrobenzofurazan Chemical compound [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12 IGHBXJSNZCFXNK-UHFFFAOYSA-N 0.000 claims abstract description 7
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- BTNMPGBKDVTSJY-UHFFFAOYSA-N keto-phenylpyruvic acid Chemical compound OC(=O)C(=O)CC1=CC=CC=C1 BTNMPGBKDVTSJY-UHFFFAOYSA-N 0.000 description 12
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- 238000013461 design Methods 0.000 description 7
- 239000001903 2-oxo-3-phenylpropanoic acid Substances 0.000 description 6
- DEDGUGJNLNLJSR-UHFFFAOYSA-N alpha-hydroxycinnamic acid Natural products OC(=O)C(O)=CC1=CC=CC=C1 DEDGUGJNLNLJSR-UHFFFAOYSA-N 0.000 description 6
- 238000001819 mass spectrum Methods 0.000 description 6
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 6
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- OJPZNRMSJHFGTR-UHFFFAOYSA-N 2-oxidanylidene-2-phenyl-ethanal Chemical compound O=CC(=O)C1=CC=CC=C1.O=CC(=O)C1=CC=CC=C1 OJPZNRMSJHFGTR-UHFFFAOYSA-N 0.000 description 2
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- WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 2
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- 230000027756 respiratory electron transport chain Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D271/00—Heterocyclic compounds containing five-membered rings having two nitrogen atoms and one oxygen atom as the only ring hetero atoms
- C07D271/12—Heterocyclic compounds containing five-membered rings having two nitrogen atoms and one oxygen atom as the only ring hetero atoms condensed with carbocyclic rings or ring systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610050322.6A CN105503768B (en) | 2016-01-26 | 2016-01-26 | The preparation method of the fluorescence of alpha ketoglutaric acids/ultraviolet molecular probe and its application in biological specimen |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610050322.6A CN105503768B (en) | 2016-01-26 | 2016-01-26 | The preparation method of the fluorescence of alpha ketoglutaric acids/ultraviolet molecular probe and its application in biological specimen |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105503768A true CN105503768A (en) | 2016-04-20 |
CN105503768B CN105503768B (en) | 2018-01-30 |
Family
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Family Applications (1)
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CN201610050322.6A Expired - Fee Related CN105503768B (en) | 2016-01-26 | 2016-01-26 | The preparation method of the fluorescence of alpha ketoglutaric acids/ultraviolet molecular probe and its application in biological specimen |
Country Status (1)
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CN (1) | CN105503768B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107382900A (en) * | 2017-06-30 | 2017-11-24 | 曲阜师范大学 | A kind of preparation method and applications of pH fluorescence probes |
CN109748970A (en) * | 2019-01-24 | 2019-05-14 | 华东理工大学 | α-ketoglutaric acid optical probe and its preparation method and application |
CN111196821A (en) * | 2018-11-16 | 2020-05-26 | 中国科学院上海药物研究所 | Compounds, preparation method thereof and application thereof as near-infrared two-region fluorescent probe for detecting methylglyoxal |
CN116148231A (en) * | 2023-02-17 | 2023-05-23 | 广东兴昶未来食品科技有限公司 | On-line monitoring system and method for freezing and preserving of prefabricated vegetables |
-
2016
- 2016-01-26 CN CN201610050322.6A patent/CN105503768B/en not_active Expired - Fee Related
Non-Patent Citations (4)
Title |
---|
JESSIE A. KEY ET AL.: "Detection of Cellular Sialic Acid Content Using Nitrobenzoxadiazole Carbonyl-Reactive Chromophores", 《BIOCONJUGATE CHEM.》 * |
PENGWEI JIN ET AL.: "Rational design of a turn-on fluorescent sensor for a-ketoglutaric acid in a microfluidic chip", 《CHEM. SCI.》 * |
YE HE ET AL.: "Optimizing the Chemical Recognition Process of a Fluorescent Chemosensor for α‑Ketoglutarate", 《IND. ENG.CHEM. RES.》 * |
焦长红等: "基于罗丹明B的激活型α-酮戊二酸荧光探针", 《影像科学与光化学》 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107382900A (en) * | 2017-06-30 | 2017-11-24 | 曲阜师范大学 | A kind of preparation method and applications of pH fluorescence probes |
CN107382900B (en) * | 2017-06-30 | 2020-05-19 | 曲阜师范大学 | Preparation method and application of pH fluorescent probe |
CN111196821A (en) * | 2018-11-16 | 2020-05-26 | 中国科学院上海药物研究所 | Compounds, preparation method thereof and application thereof as near-infrared two-region fluorescent probe for detecting methylglyoxal |
CN111196821B (en) * | 2018-11-16 | 2022-11-29 | 中国科学院上海药物研究所 | Compounds, preparation method thereof and application thereof as near-infrared two-region fluorescent probe for detecting methylglyoxal |
CN109748970A (en) * | 2019-01-24 | 2019-05-14 | 华东理工大学 | α-ketoglutaric acid optical probe and its preparation method and application |
CN109748970B (en) * | 2019-01-24 | 2022-07-05 | 华东理工大学 | Alpha-ketoglutaric acid optical probe and preparation method and application thereof |
CN116148231A (en) * | 2023-02-17 | 2023-05-23 | 广东兴昶未来食品科技有限公司 | On-line monitoring system and method for freezing and preserving of prefabricated vegetables |
CN116148231B (en) * | 2023-02-17 | 2023-09-19 | 广东兴昶未来食品科技有限公司 | On-line monitoring system and method for freezing and preserving of prefabricated vegetables |
Also Published As
Publication number | Publication date |
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CN105503768B (en) | 2018-01-30 |
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