CN105476880A - Preparation method of emulsion carrying epidermal growth factors - Google Patents
Preparation method of emulsion carrying epidermal growth factors Download PDFInfo
- Publication number
- CN105476880A CN105476880A CN201510932387.9A CN201510932387A CN105476880A CN 105476880 A CN105476880 A CN 105476880A CN 201510932387 A CN201510932387 A CN 201510932387A CN 105476880 A CN105476880 A CN 105476880A
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- CN
- China
- Prior art keywords
- epidermal growth
- growth factor
- emulsion
- preparation
- growth factors
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
- A61K8/064—Water-in-oil emulsions, e.g. Water-in-silicone emulsions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/25—Silicon; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/89—Polysiloxanes
- A61K8/891—Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Abstract
The invention discloses a preparation method of an emulsion carrying epidermal growth factors, belongs to the field of emulsion embedding, and aims to solve the problem that the epidermal growth factors are short in biological activity cycle and poor in percutaneous absorption effect. The epidermal growth factors are creatively embedded into the water-in-oil type Pickering emulsion, so that the biological stability of the epidermal growth factors is increased, and the percutaneous absorption effect of the epidermal growth factors is increased through the close-to-skin performance of the outer oil phase. Hydrophobic SiO2 and oleophylic surfactant are mixed to obtain the stable emulsion which can keep stable without layering during storage. The preparation method is novel in idea, simple in preparation, high in practicality and capable of providing a feasible scheme to effective application of water-soluble epidermal growth factors to cosmetics.
Description
Technical field
Deliver an emulsion preparation method for epidermal growth factor, specifically with hydrophobicity SiO
2with surfactant compound as emulsifying agent, liquid paraffin or liquid silicone oil are oil phase, and epidermal growth factor aqueous solution is aqueous phase, adopt high speed dispersion to prepare water-in-oil type pickering emulsion, belong to emulsion embedding techniques field.
Background technology
Epidermal growth factor (EpidermalGrowthFactor, EGF) be the little peptide material of a class be extensively present in mammalian body, be made up of 53 aminoacid, be a kind of multi-functional somatomedin, have strong short splitting action to Various Tissues cell.Show according to the study, epidermal growth factor by with cell-membrane receptor tie mutually and, the metabolism network that formation one is more complicated in intracellular delivery, the phosphorylation of inducing receptor itself, the protein tyrosine kinase activity of activated receptor, catalytic substrate protein-tyrosine residue phosphorylation simultaneously, take part in Growth of Cells and a series of biochemical process of propagation.Therefore, epidermal growth factor is usually used in medical cosmetology, and it has the effects such as skin care, skin care, reparation wound and prevention mottle.But at present, the extraction of epidermal growth factor and produce more limited, resource is few, yield poorly and expensive.In addition, epidermal growth factor is a kind of biological activity protein, and its activity in regular solution generally can only preserve 7-10 days, is therefore difficult to it can be used as the additive of cosmetics and skincare product to use.Existing commercially available epidermal growth factor list product are mostly powdered, by rapid freeze-drying technology, condense into dry powder rapidly at low temperatures, extend its activity time.
For this problem, much study the activity being improved epidermal growth factor by various chemical method, the present invention adopts water-in-oil type pickering emulsion embedding techniques, is embedded in by epidermal growth factor in the aqueous phase of pickering emulsion, reduces the probability of its inactivation.Pickering emulsion is compared with conventional emulsion, and using solid particle as emulsifying agent, not containing the emulsifying agent with certain toxic and side effects, foam is less, little to the stimulation of human body, environmentally friendly simultaneously.On the other hand, the particle interface film that particle emulsifying agents is formed, than emulsifier molecules thickness, has strong interface stability, and therefore, emulsion is not easily by the impact of the factors such as system pH, temperature and oil phase composition.Therefore, pickering emulsion has more wide using value in food, cosmetics, medicine and other fields.
Summary of the invention
The technical problem to be solved in the present invention is open a kind of emulsion preparation method delivering epidermal growth factor.Namely the epidermal growth factor water-in-oil type pickering emulsion of a kind of high activity, high stability is provided, is applied to cosmetics.
Technical scheme of the present invention: a kind of emulsion preparation method delivering epidermal growth factor, its composition is by percentage to the quality: epidermal growth factor 0.1 ~ 1%, hydrophobicity SiO
20.5 ~ 5%, surfactant 0.5 ~ 5%, oil phase 40 ~ 60%, moisture 40 ~ 60%.
The preparation method of described epidermal growth factor emulsion, comprises the following steps:
Hydrophobicity SiO in ratio described in (a) precise
2and surfactant, add and be equipped with in the container of oil phase, be uniformly mixed;
B () uses syringe that epidermal growth factor aqueous solution is slowly injected above-mentioned system under the high speed dispersor slow-speed of revolution, make thick breast, then improves rotating speed dispersion, preparation emulsifying sample.
The hydrophobicity SiO used in step (a)
2for
h13L,
h15,
h17,
h20,
h2000,
one or more in H30, surfactant is one or more in span 20, span 40, sorbester p18, sorbester p17, and oil phase is one or more in liquid silicone oil DC556, DCPMX-345, DCPMX-200, DCPMX-200;
The high speed dispersion slow-speed of revolution used in step (b) is 1000 ~ 5000rpm, and the operating time is 5 ~ 10min; High speed dispersion height rotating speed is 10000 ~ 20000rpm, and the operating time is 5 ~ 10min.
Beneficial effect of the present invention:
Select hydrophobicity SiO
2composite with span series, by high speed dispersor emulsifying, prepare the water-in-oil type pickering emulsion of delivery epidermal growth factor of the present invention.By emulsion embedding techniques of the present invention, avoid the problem of inactivation in epidermal growth factor short-term as far as possible, enhance its stability.In addition, compared with the emulsion being emulsifying agent with conventional surfactants, pickering emulsion has stronger stability and medicament slow release effect, therefore, is also easier to long-term preservation and the application of emulsion.
Accompanying drawing explanation
The w/o type Pickering emulsion appearance that the different high speed dispersion height rotating speed of Fig. 1 and time obtain (is followed successively by 10000rpm3 minute from left to right, 10000rpm5 minute, 10000rpm8 minute, 15000rpm3 minute, 15000rpm5 minute, 15000rpm8 minute, 21000rpm3 minute, 21000rpm5 minute, 21000rpm8 minute).
The hydrophobic silica of Fig. 2 variable concentrations
water-in-oil type pickering emulsion outward appearance (being followed successively by 1%, 2%, 3% and 4% from left to right) prepared by H20.
Detailed description of the invention
Embodiment 1
Getting 0.5g span 40 adds in 50mL silicone oil DC556, and heating in water bath 1h, magnetic agitation 2h, add 1g hydrophobic silica in finely dispersed oil phase
h20, whirlpool shakes, and is slowly added the aqueous solution of isopyknic 0.1% epidermal growth factor with oil phase under 3000rpm rotating speed by syringe, uses high speed dispersor high speed dispersion 3min under 10000rpm rotating speed to prepare water-in-oil type pickering emulsion.
Embodiment 2
Getting 0.5g span 40 adds in 50mL silicone oil DC556, and heating in water bath 1h, magnetic agitation 2h, add 1g hydrophobic silica in finely dispersed oil phase
h20, whirlpool shakes, and is slowly added the aqueous solution of isopyknic 0.1% epidermal growth factor with oil phase under 3000rpm rotating speed by syringe, uses high speed dispersor high speed dispersion 5min under 10000rpm rotating speed to prepare water-in-oil type pickering emulsion.
Embodiment 3
Getting 0.5g span 40 adds in 50mL silicone oil DC556, and heating in water bath 1h, magnetic agitation 2h, add 1g hydrophobic silica in finely dispersed oil phase
h20, whirlpool shakes, and is slowly added the aqueous solution of isopyknic 0.1% epidermal growth factor with oil phase under 3000rpm rotating speed by syringe, uses high speed dispersor high speed dispersion 3min under 15000rpm rotating speed to prepare water-in-oil type pickering emulsion.
Embodiment 4
Getting 0.5g span 40 adds in 50mL silicone oil DC556, and heating in water bath 1h, magnetic agitation 2h, add 1g hydrophobic silica in finely dispersed oil phase
h20, whirlpool shakes, and is slowly added the aqueous solution of isopyknic 0.1% epidermal growth factor with oil phase under 3000rpm rotating speed by syringe, uses high speed dispersor high speed dispersion 3min under 21000rpm rotating speed to prepare water-in-oil type pickering emulsion.
Embodiment 5
Getting 0.5g span 40 adds in 50mL silicone oil DC556, and heating in water bath 1h, magnetic agitation 2h, add 0.5g hydrophobic silica in finely dispersed oil phase
h20, whirlpool shakes, and is slowly added the aqueous solution of isopyknic 0.1% epidermal growth factor with oil phase under 3000rpm rotating speed by syringe, adopts high speed dispersor high speed dispersion 5min under 15000rpm rotating speed to prepare water-in-oil type pickering emulsion.
Embodiment 6
Getting 0.5g span 40 adds in 50mL silicone oil DC556, and heating in water bath 1h, magnetic agitation 2h, add 1.5g hydrophobic silica in finely dispersed oil phase
h20, whirlpool shakes, and is slowly added the aqueous solution of isopyknic 0.1% epidermal growth factor with oil phase under 3000rpm rotating speed by syringe, adopts high speed dispersor high speed dispersion 5min under 15000rpm rotating speed to prepare water-in-oil type pickering emulsion.
Embodiment 7
Getting 0.5g span 40 adds in 50mL silicone oil DC556, and heating in water bath 1h, magnetic agitation 2h, add 2g hydrophobic silica in finely dispersed oil phase
h20, whirlpool shakes, and is slowly added the aqueous solution of isopyknic 0.1% epidermal growth factor with oil phase under 3000rpm rotating speed by syringe, adopts high speed dispersor high speed dispersion 5min under 15000rpm rotating speed to prepare water-in-oil type pickering emulsion.
In embodiment 1, embodiment 2, embodiment 3 and embodiment 4, by regulating speed and the time of high speed dispersion high speed, all can obtain stable water-in-oil type pickering emulsion, its emulsion appearance as shown in Figure 1.In embodiment 5, embodiment 6 and embodiment 7, by using the hydrophobic silica of variable concentrations
h20, all can obtain stable water-in-oil type pickering emulsion, its emulsion appearance as shown in Figure 2.Be used for simulating in transdermal experiment by obtained pickering emulsion, contrast with ordinary emulsion, the infiltration capacity delivering the epidermal growth factor in water-in-oil type pickering emulsion is doubled.
Claims (4)
1. deliver an emulsion preparation method for epidermal growth factor, it is characterized in that its composition is by percentage to the quality: epidermal growth factor 0.1 ~ 1%, hydrophobicity SiO
20.5 ~ 5%, surfactant 0.4 ~ 5%, oil phase 40 ~ 60%, moisture 40 ~ 60%.
2. a kind of emulsion preparation method delivering epidermal growth factor according to claim 1, is characterized in that comprising the following steps:
Hydrophobicity SiO in ratio described in (a) precise
2and surfactant, add and be equipped with in the container of oil phase, be uniformly mixed;
B () uses syringe that epidermal growth factor aqueous solution is slowly injected above-mentioned system under the high speed dispersor slow-speed of revolution, make thick breast, then improves rotating speed dispersion, preparation emulsifying sample.
3. a kind of emulsion preparation method delivering epidermal growth factor according to claim 2, is characterized in that:
The hydrophobicity SiO used in step (a)
2for
in one or more, surfactant is one or more in span 20, span 40, sorbester p18, sorbester p17, and oil phase is one or more in liquid silicone oil DC556, DCPMX-345, DCPMX-200, DCPMX-200;
The high speed dispersion slow-speed of revolution used in step (b) is 1000 ~ 5000rpm, and the operating time is 5 ~ 10min; High speed dispersion height rotating speed is 10000 ~ 20000rpm, and the operating time is 5 ~ 10min.
4. one kind delivers the emulsion of epidermal growth factor as claimed in claim 1, it is characterized in that epidermal growth factor to be embedded in the aqueous phase of water-in-oil type pickering emulsion, emulsion particle is uniformly dispersed, storage stability is good, epidermal growth factor stability in the product can be improved, and improve its skin absorption efficiency.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510932387.9A CN105476880A (en) | 2015-12-15 | 2015-12-15 | Preparation method of emulsion carrying epidermal growth factors |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510932387.9A CN105476880A (en) | 2015-12-15 | 2015-12-15 | Preparation method of emulsion carrying epidermal growth factors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105476880A true CN105476880A (en) | 2016-04-13 |
Family
ID=55664400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510932387.9A Pending CN105476880A (en) | 2015-12-15 | 2015-12-15 | Preparation method of emulsion carrying epidermal growth factors |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006018112A1 (en) * | 2004-08-12 | 2006-02-23 | Wacker Chemie Ag | Rheology control of pickering emulsions by electrolytes |
| CN104403117A (en) * | 2014-12-08 | 2015-03-11 | 江南大学 | Pickering emulsion as well as preparation method thereof |
-
2015
- 2015-12-15 CN CN201510932387.9A patent/CN105476880A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006018112A1 (en) * | 2004-08-12 | 2006-02-23 | Wacker Chemie Ag | Rheology control of pickering emulsions by electrolytes |
| CN104403117A (en) * | 2014-12-08 | 2015-03-11 | 江南大学 | Pickering emulsion as well as preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| ALLA NESTERENKO等: "Influence of a mixed particle/surfactant emulsifier system on water-in-oil emulsion stability", 《COLLOIDS AND SURFACES A: PHYSICOCHEMICAL AND ENGINEERING ASPECTS》 * |
| 冯康等: "变性淀粉在Pickering 乳液中应用", 《粮食与油脂》 * |
| 杨志明: "《组织工程》", 30 September 2002 * |
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Application publication date: 20160413 |
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