CN105476728A - Experiment device for inducing mouse cardiopulmonary vascular diseases - Google Patents
Experiment device for inducing mouse cardiopulmonary vascular diseases Download PDFInfo
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- CN105476728A CN105476728A CN201610084731.8A CN201610084731A CN105476728A CN 105476728 A CN105476728 A CN 105476728A CN 201610084731 A CN201610084731 A CN 201610084731A CN 105476728 A CN105476728 A CN 105476728A
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- shaped
- extracting box
- fume extracting
- hole
- smoking
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
- A61D7/04—Devices for anaesthetising animals by gases or vapours; Inhaling devices
Abstract
An experiment device for inducing mouse cardiopulmonary vascular diseases is mainly used for building common cigarette induced mouse cardiopulmonary vascular disease models. The experiment device comprises a smoking box, a support, mouse bins and a draught fan. The smoking box is bonded on the T-shaped support, the draught fan is installed at the left end of the smoking box, a smoke concentration induction probe is arranged on the smoking box and on the right side of the draught fan, a gas inlet plate is bonded at the right end of the smoking box, a dispersing plate is bonded in the smoking box and on the left side of the gas inlet plate, and 10-12 tapered round holes are formed in the middle of the smoking box in a front-back symmetrical mode. One mouse bin is inserted into each tapered round hole. The experiment device has the advantages that the structure is simple, usage is convenient, and the smoke concentration is adjustable and distributed evenly; mice only smoke through mouths and noses, and other parts of bodies of the mice are not in the smoke, so that the smoking mode of the mice is closer to a human smoking mode, reliability of experiment data is greatly improved, and differences of experiment results are decreased.
Description
Technical field
The present invention relates to a kind of medical experiment utensil, particularly a kind of experimental provision bringing out mouse core pulmonary vascular disease, it is mainly used in setting up when common cigarette brings out mouse core pulmonary vascular disease model and uses.
Background technology
Cardiopulmonary and vascular disease is that height is disabled fatal disease, and it and Aortic Plaque break, platelet activation, Coagulation Fibrinolysis functional imbalance cause artery thrombosis closely related.Smoking is one of lethal most important risk factor of cardiopulmonary and vascular disease.The clinical person that the sees healthy smoking Case report that a large amount of smoking burst cardiopulmonary and vascular disease is lethal in a short time.Cause cardiopulmonary and vascular disease to there is no effective preventive measure to smoking at present, but essential measure is smoking cessation.Scientific research shows, and smoking can make the lethal risk of non-lethality cardiopulmonary and vascular disease increase by 3 times, and it is closely related that its pathophysiological mechanism and smoking cause cardiopulmonary vascular thrombosis to be formed.Smoking causes thrombotic mechanism probably to comprise: smoking causes platelet activation, and mechanism generates NO with endothelial injury and reduces relevant; Endotheliocyte plasminogen activating factors (tPA) generates minimizing and Plasminogen Activator Inhibitor (PAI-I) generation increase promotes that blood is in hypercoagulability; Atherosclerotic plaque place tissue factor, VCAM-1 generate increase, and mononuclear phagocyte assembles increase; The thromboxane A2 that smoking causes intra platelet free calcium a large amount of, makes platelet adhesion aggregation rate increase; Smoking causes speckle unstable.
In order to study the impact of smoking on human lung, being by setting up smoking mice cardiopulmonary and vascular disease model usually mostly, inquiring into the change of blood coagulation in blood, fibrinolytic factor before and after smoking mice smoking and vascular endothelial function change, blood vessel change.Want to set up smoking mice cardiopulmonary and vascular disease model, just must use mouse tobacco smoke experimental provision, the smoking experimental provision used in current smoking mice cardiopulmonary and vascular disease model is roughly divided into two kinds, the first experimental provision comprises air pump, constant pressure valve, permanent flow valve, fume collection device, flue gas blender, fume extracting box, this smoking experimental provision complex structure, the smokescope finally delivered in fume extracting box is less than 3%, this will strengthen the test period, and the test data diversity of experimental group and matched group is not remarkable, affects result of the test; The second experimental provision is only made up of fume extracting box and air pump, fume extracting box top is provided with a Medicated cigarette socket, Medicated cigarette is inserted on this socket, a small-sized air exhauster is communicated with in the bottom of fume extracting box, the shortcoming of this experimental provision be in fume extracting box smokescope and wind speed uneven, the cigarette inhaled by the mice of causing is many, and the cigarette that some mices inhale is few, and its result of the test diversity is larger.Another common drawback of above-mentioned two kinds of experimental provisions is that fume extracting box design is unreasonable, during experiment, several mices are put into fume extracting box together, when mice smells smog abnormal smells from the patient, will health be shunk, mutually gather around and squeeze a ball, the nostril of oneself is hidden among fur, so greatly have impact on the effect of actual nostril smoking, make nostril smoking become fur smoking, make experimental data inaccurate, unreliable.Again because the position that every mice is residing in fume extracting box is different, the smoking capacity on historical facts or anecdotes border is also not quite similar, and also increases the diversity of result of the test like this.
Summary of the invention
The object of the invention is to the defect and the deficiency that make up prior art, a kind of experimental provision bringing out mouse core pulmonary vascular disease is provided, its structure is simple, and easy to use, smokescope is adjustable, smokescope is evenly distributed, allow every mice in experimental group can only use mouth and nose smoking, health fur does not contact smog, makes the smoking regime of smoking regime closer to the mankind of mice, so both can reduce the diversity of result of the test, the reliability of experimental data can have been improved again.
For achieving the above object, the technical solution used in the present invention is as follows: a kind of experimental provision bringing out mouse core pulmonary vascular disease, comprise fume extracting box, support, Mus storehouse, blower fan, described fume extracting box, support, Mus storehouse is made by water white lucite, be conducive to like this observing the deployment conditions of smog and the tobacco smoking status of mice in fume extracting box, wherein fume extracting box is bonded on T-shaped support, fume extracting box is a cylinder, length is 400 ~ 600 millimeters, internal diameter is 50 ~ 80 millimeters, fume extracting box left end is equipped with an air exhauster, a smokescope inductive probe fume extracting box on the right side of air exhauster is equipped with, an inlet plate is bonded with at fume extracting box right-hand member, the middle part of inlet plate is shaped with a smoke intaking hole, the periphery of smoke intaking hole is shaped with 4 for entering the fresh air inlet of air, a diffuser plate is bonded with in fume extracting box on the left of inlet plate, diffuser plate is shaped with several aperture uniformly, the effect of diffuser plate is in the fume extracting box be distributed to uniformly on the left of diffuser plate by smog of coming from fume extracting box right-hand member, before and after in the middle part of fume extracting box, symmetry is shaped with 10 ~ 12 taper circular holes, a Mus storehouse is fitted with in each taper circular hole, Mus storehouse main part is one section of pipe, the one end in Mus storehouse is shaped with a cylindrical cone matched with taper circular hole, a cone-shaped inner hole is shaped with in this cylindrical cone, the size of this cone-shaped inner hole just in time can hold the head of a mice, and the snoot of mice can be made to reach in fume extracting box, the other end in Mus storehouse is connected with a Mus Cang Gai through screw thread, and the side of Mus Cang Gai is shaped with together for dodging the open slot of mousetail, be equipped with one at fume extracting box right-hand member and adjust wind lid, the middle part of this tune wind lid is shaped with one for placing the tobacco insert hole of medicated cigarette, tobacco insert hole communicates with smoke intaking hole, be shaped with 4 for entering the wind adjusting hole of air at the periphery of this tobacco insert hole, the size of this wind adjusting hole is identical with the fresh air inlet of 4 on inlet plate with orientation, the inlet of air can be changed when rotating and adjusting wind lid, adjust the concentration of smog in fume extracting box with this.
Using method of the present invention is: this device is placed on the table, mice is loaded Mus storehouse, and Mus Cang Gai is built, the snoot of mice is just in time stretched out from cone-shaped inner hole, light at tobacco insert hole place inserted into cigarette, open air exhauster by this device of smoke inhalation, when smokescope is undesirable in fume extracting box, by rotating the concentration adjusting smog in wind lid and adjustable fume extracting box.After mouse tobacco smoke completes, Mus storehouse can be extracted from fume extracting box, open Mus Cang Gai, mice is refunded in mouse cage.
The present invention compared with prior art beneficial effect is: its structure is simple, easy to use, smokescope is adjustable, smokescope is evenly distributed, and mice only uses mouth and nose smoking, and the other parts of its health are not in smog, make the smoking regime of smoking regime closer to the mankind of mice, substantially increase the reliability of experimental data like this, reduce the diversity of result of the test, for the mechanism of next step research Induced By Tobacco Smoke cardiovascular disease lays a solid foundation.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is that the A-A sectional view in Fig. 1 rotates.
Fig. 3 is the A direction view of Mus Cang Gai in Fig. 1.
Fig. 4 is the B direction view adjusting wind lid in Fig. 1.
Fig. 5 is the left view of inlet plate in Fig. 1.
Fig. 6 is the profile in Mus storehouse in Fig. 1.
In figure, 1. support, 2. fume extracting box, 3. Mus storehouse, 4. Mus Cang Gai, 5. diffuser plate, 6. inlet plate, 7. adjusts wind lid, 8. tobacco insert hole, 9. wind adjusting hole, 10. open slot, 11. inductive probes, 12. taper circular holes, 13. cone-shaped inner holes, 14. air exhausters, 15. smoke intaking holes, 16. fresh air inlets, 17. cylindrical cones.
Detailed description of the invention
In figure, a kind of experimental provision bringing out mouse core pulmonary vascular disease, comprise fume extracting box 2, support 1, Mus storehouse 3, air exhauster 14, described fume extracting box 2, support 1, Mus storehouse 3 is made by water white lucite, wherein fume extracting box 2 is bonded on T-shaped support 1, fume extracting box 2 is a cylinder, length is 400 ~ 600 millimeters, internal diameter is 50 ~ 80 millimeters, fume extracting box 2 left end is equipped with an air exhauster 14, a smokescope inductive probe 11 fume extracting box 2 on the right side of air exhauster 14 is equipped with, an inlet plate 6 is bonded with at fume extracting box 2 right-hand member, the middle part of inlet plate 6 is shaped with a smoke intaking hole 15, the periphery of smoke intaking hole is shaped with 4 for entering the fresh air inlet 16 of air, a diffuser plate 5 is bonded with in fume extracting box 2 on the left of inlet plate 6, several aperture is shaped with on diffuser plate 5 is uniform, before and after in the middle part of fume extracting box 2, symmetry is shaped with 10 ~ 12 taper circular holes 12, a Mus storehouse 3 is fitted with in each taper circular hole 12, Mus storehouse 3 main part is one section of pipe, the one end in Mus storehouse 3 is shaped with a cylindrical cone 17 matched with taper circular hole 12, a cone-shaped inner hole 13 is shaped with in this cylindrical cone 17, the size of this cone-shaped inner hole 13 just in time can hold the head of a mice, the other end in Mus storehouse 3 is connected with a Mus storehouse lid 4 through screw thread, and the side of Mus storehouse lid 4 is shaped with together for dodging the open slot 10 of mousetail, be equipped with one at fume extracting box 2 right-hand member and adjust wind lid 7, the middle part of this tune wind lid 7 is shaped with one for placing the tobacco insert hole 8 of medicated cigarette, tobacco insert hole 8 communicates with smoke intaking hole 15, be shaped with 4 for entering the wind adjusting hole 9 of air at the periphery of this tobacco insert hole 8, the size of this wind adjusting hole 9 is identical with the fresh air inlet 16 of 4 on inlet plate 6 with orientation, the inlet of air can be changed when rotating and adjusting wind lid 7, adjust the concentration of smog in fume extracting box 2 with this.
Claims (4)
1. one kind is brought out the experimental provision of mouse core pulmonary vascular disease, it is characterized in that: comprise fume extracting box, support, Mus storehouse, blower fan, wherein fume extracting box is bonded on T-shaped support, fume extracting box is a cylinder, fume extracting box left end is equipped with an air exhauster, a smokescope inductive probe fume extracting box on the right side of air exhauster is equipped with, an inlet plate is bonded with at fume extracting box right-hand member, the middle part of inlet plate is shaped with a smoke intaking hole, the periphery of smoke intaking hole is shaped with 4 for entering the fresh air inlet of air, a diffuser plate is bonded with in fume extracting box on the left of inlet plate, diffuser plate is shaped with several aperture uniformly, before and after in the middle part of fume extracting box, symmetry is shaped with 10 ~ 12 taper circular holes, a Mus storehouse is fitted with in each taper circular hole, Mus storehouse main part is one section of pipe, the one end in Mus storehouse is shaped with a cylindrical cone matched with taper circular hole, a cone-shaped inner hole is shaped with in this cylindrical cone, the size of this cone-shaped inner hole just in time can hold the head of a mice, and the snoot of mice can be made to reach in fume extracting box, the other end in Mus storehouse is connected with a Mus Cang Gai through screw thread, be equipped with one at fume extracting box right-hand member and adjust wind lid, the middle part of this tune wind lid is shaped with one, and for placing the tobacco insert hole of medicated cigarette, this tobacco insert hole communicates with above-mentioned smoke intaking hole, is shaped with 4 for entering the wind adjusting hole of air at the periphery of this tobacco insert hole.
2. a kind of experimental provision bringing out mouse core pulmonary vascular disease according to claim 1, it is characterized in that: described fume extracting box length is 400 ~ 600 millimeters, internal diameter is 50 ~ 80 millimeters.
3. a kind of experimental provision bringing out mouse core pulmonary vascular disease according to claim 1, is characterized in that: the side of described Mus Cang Gai is shaped with together for dodging the open slot of mousetail.
4. a kind of experimental provision bringing out mouse core pulmonary vascular disease according to claim 1, is characterized in that: the size of described 4 wind adjusting holes is identical with the fresh air inlet of 4 on inlet plate with orientation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610084731.8A CN105476728A (en) | 2016-02-15 | 2016-02-15 | Experiment device for inducing mouse cardiopulmonary vascular diseases |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610084731.8A CN105476728A (en) | 2016-02-15 | 2016-02-15 | Experiment device for inducing mouse cardiopulmonary vascular diseases |
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| CN105476728A true CN105476728A (en) | 2016-04-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610084731.8A Pending CN105476728A (en) | 2016-02-15 | 2016-02-15 | Experiment device for inducing mouse cardiopulmonary vascular diseases |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109091268A (en) * | 2018-08-23 | 2018-12-28 | 四川大学华西医院 | COPD intranasal sootiness mouse modeling device |
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| US5099792A (en) * | 1991-04-08 | 1992-03-31 | Battelle Memorial Institute | Interchangeable whole-body and nose-only exposure system |
| US5109797A (en) * | 1990-08-29 | 1992-05-05 | Briant James K | Avian inhalation exposure chamber |
| US5297502A (en) * | 1993-05-05 | 1994-03-29 | Jaeger Rudolf J | Modular reversible-flow-past nasopulmonary respiratory dosing system for laboratory animals |
| US6651587B1 (en) * | 2002-03-29 | 2003-11-25 | Battelle Memorial Institute | Container for containing an experimental test animal |
| EP2095791A1 (en) * | 2008-02-29 | 2009-09-02 | Philip Morris Products S.A. | Exposure apparatus |
| CN201477092U (en) * | 2009-08-28 | 2010-05-19 | 上海烟草（集团）公司 | Animal fumigation system for a cigarette fume toxicology research |
| CN202654264U (en) * | 2012-04-19 | 2013-01-09 | 宁波大学 | Device for manufacturing animal model of smoking chronic obstructive pulmonary disease |
| CN103505302A (en) * | 2012-06-26 | 2014-01-15 | 赵菁 | Experimental device for inducing pulmonary arterial hypertension of mouse |
| CN203749643U (en) * | 2013-11-15 | 2014-08-06 | 北京慧荣和科技有限公司 | Exposure equipment for smoke inhaled by mouth and nose of animal |
| CN104605960A (en) * | 2015-02-26 | 2015-05-13 | 赵菁 | Experimental apparatus for studying cardiovascular morbidity mechanism |
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2016
- 2016-02-15 CN CN201610084731.8A patent/CN105476728A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0121795A2 (en) * | 1983-03-10 | 1984-10-17 | Stauffer Chemical Company | Inhalation exposure apparatus |
| US4860741A (en) * | 1987-02-09 | 1989-08-29 | Research & Consulting Co. A.G. | Modular inhalation tower for laboratory animals |
| US5109797A (en) * | 1990-08-29 | 1992-05-05 | Briant James K | Avian inhalation exposure chamber |
| US5099792A (en) * | 1991-04-08 | 1992-03-31 | Battelle Memorial Institute | Interchangeable whole-body and nose-only exposure system |
| US5297502A (en) * | 1993-05-05 | 1994-03-29 | Jaeger Rudolf J | Modular reversible-flow-past nasopulmonary respiratory dosing system for laboratory animals |
| US6651587B1 (en) * | 2002-03-29 | 2003-11-25 | Battelle Memorial Institute | Container for containing an experimental test animal |
| EP2095791A1 (en) * | 2008-02-29 | 2009-09-02 | Philip Morris Products S.A. | Exposure apparatus |
| CN201477092U (en) * | 2009-08-28 | 2010-05-19 | 上海烟草（集团）公司 | Animal fumigation system for a cigarette fume toxicology research |
| CN202654264U (en) * | 2012-04-19 | 2013-01-09 | 宁波大学 | Device for manufacturing animal model of smoking chronic obstructive pulmonary disease |
| CN103505302A (en) * | 2012-06-26 | 2014-01-15 | 赵菁 | Experimental device for inducing pulmonary arterial hypertension of mouse |
| CN203749643U (en) * | 2013-11-15 | 2014-08-06 | 北京慧荣和科技有限公司 | Exposure equipment for smoke inhaled by mouth and nose of animal |
| CN104605960A (en) * | 2015-02-26 | 2015-05-13 | 赵菁 | Experimental apparatus for studying cardiovascular morbidity mechanism |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109091268A (en) * | 2018-08-23 | 2018-12-28 | 四川大学华西医院 | COPD intranasal sootiness mouse modeling device |
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Application publication date: 20160413 |
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