CN105475269A - Novel cell cryopreservation box - Google Patents
Novel cell cryopreservation box Download PDFInfo
- Publication number
- CN105475269A CN105475269A CN201610032792.XA CN201610032792A CN105475269A CN 105475269 A CN105475269 A CN 105475269A CN 201610032792 A CN201610032792 A CN 201610032792A CN 105475269 A CN105475269 A CN 105475269A
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- China
- Prior art keywords
- box body
- lid
- hole slot
- box
- boss
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Packages (AREA)
- Packging For Living Organisms, Food Or Medicinal Products That Are Sensitive To Environmental Conditiond (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a novel cell cryopreservation box which comprises a box body (1) and a box cover (4), wherein a groove (2) is formed in the box body (1); a plurality of box body hole grooves (3) are formed in the groove (2); a boss (5) is arranged on the box cover (4); a plurality of box cover hole grooves (6) are formed in the boss (5); the groove (2) and the boss (5) can be mounted in a matched manner; the height of the boss (5) is greater than or equal to the depth of the groove (2); the box body hole grooves (3) correspond to the box cover hole grooves (6); the box body (1) is a polyurethane box body; the box cover (4) is a polyurethane box cover. The novel cell cryopreservation box disclosed by the invention has the benefits as follows: the novel cell cryopreservation box has the advantages that cleanness and environment friendliness are realized, other objects are not required to be added, precooling and maintenance cost are not required, and high convenience, practicability, economy and the like are achieved; used polyurethane is an excellent freezing and slow-cooling material, so that the good slow-cooling rate can be provided, freezing damage to cells is reduced, and the activity and recovery survival rate of cryopreserved cells are improved.
Description
Technical field
The present invention relates to cell cryopreservation box, specifically, is a kind of novel cell freezing storing box.
Background technology
In vitro in training, cell must extend along with life span, and passage number increases, and biological property can change gradually, in order to long-term cell preservation is active, or protection rare cell, often need cell to carry out freezen protective.
Frozen principle: when not adding direct freeze-stored cell under any condition, the water of intraor extracellular can form ice crystal, cause in cell and a series of change occurs, as mechanical damage, electrolyte concentration rising, osmotic pressure change, PH change, albuminous degeneration etc., can cell death be caused.Add protectant (as dimethyl sulfoxide (DMSO), it is to cytotoxic, and molecular weight is little, and solvability is large, easy penetration cell) to culture fluid, cell membrane can be increased to the permeability of water, the freezing point reducing water in cell.Under the freezing conditions of slow cooling, ICW can be made before freezing to appear extracellular, freezing in extracellular formation, thus avoid the infringement to cell.Therefore the vigor of slow cooling to summary freeze-stored cell seems particularly important.
Cell cryopreservation mode conventional at present has two kinds.The frozen mode of tradition first places 30-60 minute at 4 DEG C, proceeds to-20 DEG C and place 30-60 minute, then proceed to-80 DEG C of placements 16-18 hour (or spending the night), finally puts into the medium-term and long-term preservation of liquid nitrogen.Tradition frozen mode step many, consuming time length, the time length being particularly placed on 4 DEG C and-20 DEG C is not easily held, and rate of temperature fall is uneven, relatively large to the damage of cell, and after recovery, survival rate is not high.Another kind is program temperature reduction box, gradient cooling box that market is sold, puts into wherein by the cell being added with cryopreserving liquid, directly put into-80 DEG C spend the night after, then proceed to the medium-term and long-term preservation of liquid nitrogen, good slow cooling speed can be provided.But gradient cooling box expense is expensive, and need to add isopropyl alcohol.Isopropyl alcohol is poisonous, volatile, needs to be placed on Refrigerator store; Need after using 5-6 time to change isopropyl alcohol, at every turn lossy and waste, maintenance cost is high; Reuse interval time long, about 1 hour, use and need to observe liquid scale.
In order to realize slow cooling, reduce the freezing infringement to cell, improve freeze-stored cell vigor and recovery survival rate, can reduce costs again, a kind of novel cell of necessary development freezing storing box solves above deficiency.
Summary of the invention
The object of the present invention is to provide a kind of novel cell freezing storing box.
The present invention is achieved through the following technical solutions: a kind of novel cell freezing storing box, comprise box body and lid, box body is provided with groove, and be provided with several box body hole slots in groove, lid is provided with boss, boss is provided with several lid hole slots, groove can coordinate with boss to be installed, and the height of boss is more than or equal to the degree of depth of groove, and box body hole slot can be corresponding with lid hole slot, box body is polyurethane box body, and lid is polyurethane lid.
Described box body is cuboid, and lid is cuboid, and box body hole slot becomes matrix to be uniformly distributed with lid hole slot.
Described groove and boss are cuboid-type.
Described box body hole slot distance box body side and the distance of bottom surface are more than or equal to 3cm, and box cover hole slot pitch is more than or equal to 3cm from the distance of lid side and bottom surface.
Described box body hole slot and lid hole slot are for holding cryopreservation tube, and the diameter of box body hole slot and lid hole slot is more than or equal to the maximum gauge of cryopreservation tube.
The degree of depth of described box body hole slot and the degree of depth sum of lid hole slot are more than or equal to the height of cryopreservation tube.
Described box body hole slot and lid hole slot are blind hole.
Described box body and lid can be arranged on outer that hard material makes and protect in box, and protecting box outward can fasten.
The present invention compared with prior art, has the following advantages and beneficial effect:
(1) material of the present invention is polyurethane, it has clean, environmental protection, without the need to adding other article, do not need precooling, Maintenance free cost, the advantage such as convenient and practical, economical and practical;
(2) the present invention has superior water resistance and anti-moisture performance, does not need extra insulation and moisture;
(3) thermal conductivity factor of the present invention is very low, have very superior heat-insulating property, polyurethane used in the present invention is the excellent material of freezing slow cooling, can provide good slow cooling speed, reduce the freezing infringement to cell, improve freeze-stored cell vigor and recovery survival rate.
Accompanying drawing explanation
Fig. 1 is the structural representation of box body;
Fig. 2 is the structural representation of lid;
Fig. 3 is the assembling schematic diagram of freezing storing box and outer box;
Wherein 1-box body, 2-groove, 3-box body hole slot, 4-lid, 5-boss, 6-lid hole slot, 7-protect box outward.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1:
As shown in Figure 1, a kind of novel cell freezing storing box, comprise box body 1 and lid 4, box body 1 is provided with groove 2, several box body hole slots 3 are provided with in groove 2, lid 4 is provided with boss 5, boss 5 is provided with several lid hole slots 6, groove 2 can coordinate with boss 5 to be installed, the height of boss 5 is more than or equal to the degree of depth of groove 2, box body hole slot 3 can be corresponding with lid hole slot 6, box body 1 is polyurethane box body, lid 4 is polyurethane lid, cryopreserving liquid containing cell is loaded in cryopreservation tube, cryopreservation tube puts into box body hole slot 3, again box body 1 and lid 4 are closed, boss 5 embeds in groove 2 just, box body hole slot 3 and lid hole slot 6 one_to_one corresponding are to seal up for safekeeping in box body hole slot 3 with lid hole slot 6 by cryopreservation tube, box body 1 and lid 4 are exerted pressure and makes it to compress, because polyurethane has certain elasticity, and there is certain interval between box body 1 and lid 4, make the gap between box body 1 and lid 4 eliminate by exerting pressure, the seal of freezing storing box can be ensured with this, this freezing storing box directly can be put into-80 DEG C of low temperature refrigerators subsequently, freezing storing box can be taken out from-80 DEG C of refrigerators after 24-48 hour, proceed to the medium-term and long-term preservation of liquid nitrogen again, because polyurethane thermal conductivity factor is very low, there is very superior heat-insulating property, good slow cooling speed can be provided, reduce the freezing infringement to cell, improve freeze-stored cell vigor and recovery survival rate.
Described box body 1 is cuboid, lid 4 is cuboid, box body hole slot 3 and lid hole slot 6 one-tenth matrixes are uniformly distributed, described groove 2 is cuboid-type with boss 5, in the present embodiment, the degree of depth of groove 2 is 1cm, the height of boss 5 is 1.5cm, the height of lid 4 is 3cm, the height of box body 1 is the degree of depth+4cm of box body hole slot 3, and box body hole slot 3 matrix in 4*3 is uniformly distributed, and two adjacent box body hole slot 3 spacing distances are 2cm, it is 1cm that outermost one encloses box body hole slot 3 with the distance of groove 2, and the diameter of box body hole slot 3 is the maximum gauge+1mm of cryopreservation tube.
In the present embodiment, it is 3cm that outermost one encloses box body hole slot 3 apart from the beeline of box body 1 side, and the beeline of distance from bottom box body 1 bottom surface of box body hole slot 3 is 3cm.It is 3cm that outermost one encloses lid hole slot 6 apart from the beeline of lid 4 side, the beeline of distance from bottom lid 4 bottom surface of lid hole slot 3 is 3cm, can ensure that the beeline of the cryopreservation tube distance external environment put into is more than or equal to 3 centimetres with this, and because box body 1 and lid 4 are made up of polyurethane, the reduction per minute of fluid temperature in cryopreservation tube 1 DEG C can be made, until be reduced to-80 DEG C, cell recovery survival rate can be made to 99.5% with this, under equal experiment condition, during cryopreservation tube distance external environment 2cm, cell recovery survival rate is 97%, during cryopreservation tube distance external environment 1cm, cell recovery survival rate is 95%.
The height of described boss 5 is more than or equal to the degree of depth of groove 2, and in the present embodiment, the degree of depth of groove 2 is 1cm, and the height of boss 5 is 1.5cm.
Described box body hole slot 3 and lid hole slot 6 are for holding cryopreservation tube, the diameter of box body hole slot 3 and lid hole slot 6 is all more than or equal to the maximum gauge of cryopreservation tube, in the present embodiment, the diameter of box body hole slot 3 is the maximum gauge+1mm of cryopreservation tube, and the diameter of lid hole slot 6 is the maximum gauge+1mm of cryopreservation tube.
The degree of depth of described box body hole slot 3 and the degree of depth sum of lid hole slot 6 are more than or equal to the height of cryopreservation tube.In the present embodiment, the degree of depth of box body hole slot 3 and the degree of depth sum of lid hole slot 6 equal the height+5mm of cryopreservation tube, and the box body hole slot degree of depth is the height of cryopreservation tube whole height-cryopreservation tube lid, and box cover hole groove depth is the height+5mm of cryopreservation tube lid.Because of the height that the box body hole slot degree of depth is cryopreservation tube whole height-cryopreservation tube lid, after opening freezing storing box, the lid of cryopreservation tube exceeds box body inner face, is easy to cryopreservation tube from wherein taking out.
Described box body hole slot 3 and lid hole slot 6 are blind hole.
Embodiment 2:
As shown in Figure 3, in the present embodiment, described box body 1 and lid 4 can be arranged on outer that hard material makes and protect in box 7, protect box 7 outward can fasten, outer protect box 7 by using, can compress box body 1 and lid 4 to ensure the seal of this freezing storing box by the closed outer box 7 that protects, it is simple to operate.
The above is only preferred embodiment of the present invention, and not do any pro forma restriction to the present invention, every any simple modification, equivalent variations done above embodiment according to technical spirit of the present invention, all falls within protection scope of the present invention.
Claims (8)
1. a novel cell freezing storing box, it is characterized in that: comprise box body (1) and lid (4), box body (1) is provided with groove (2), several box body hole slots (3) are provided with in groove (2), lid (4) is provided with boss (5), boss (5) is provided with several lid hole slots (6), groove (2) can coordinate with boss (5) to be installed, the height of boss (5) is more than or equal to the degree of depth of groove (2), box body hole slot (3) can be corresponding with lid hole slot (6), box body (1) is polyurethane box body, and lid (4) is polyurethane lid.
2. a kind of novel cell freezing storing box according to claim 1, is characterized in that: described box body (1) is cuboid, and lid (4) is cuboid, and box body hole slot (3) becomes matrix to be uniformly distributed with lid hole slot (6).
3. a kind of novel cell freezing storing box according to claim 1, is characterized in that: described groove (2) and boss (5) are cuboid-type.
4. a kind of novel cell freezing storing box according to claim 1, it is characterized in that: described box body hole slot (3) distance box body (1) side and the distance of bottom surface are more than or equal to 3cm, and the distance of lid hole slot (6) distance lid (4) side and bottom surface is more than or equal to 3cm.
5. a kind of novel cell freezing storing box according to claim 1, it is characterized in that: described box body hole slot (3) and lid hole slot (6) are for holding cryopreservation tube, and the diameter of box body hole slot (3) and lid hole slot (6) is more than or equal to the maximum gauge of cryopreservation tube.
6. a kind of novel cell freezing storing box according to claim 1, is characterized in that: the degree of depth of described box body hole slot (3) and the degree of depth sum of lid hole slot (6) are more than or equal to the height of cryopreservation tube.
7. a kind of novel cell freezing storing box according to claim 1, is characterized in that: described box body hole slot (3) and lid hole slot (6) are blind hole.
8. a kind of novel cell freezing storing box according to claim 1, is characterized in that: described box body (1) and lid (4) can be arranged on outer that hard material makes and protect in box (7), and protecting box (7) outward can fasten.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610032792.XA CN105475269B (en) | 2016-01-19 | 2016-01-19 | A kind of novel cell freezing storing box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610032792.XA CN105475269B (en) | 2016-01-19 | 2016-01-19 | A kind of novel cell freezing storing box |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105475269A true CN105475269A (en) | 2016-04-13 |
| CN105475269B CN105475269B (en) | 2018-04-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610032792.XA Expired - Fee Related CN105475269B (en) | 2016-01-19 | 2016-01-19 | A kind of novel cell freezing storing box |
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| Country | Link |
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| CN (1) | CN105475269B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107629941A (en) * | 2017-10-27 | 2018-01-26 | 中国科学院理化技术研究所 | A kind of cell cryopreservation device and its application based on micro-fluidic chip |
| CN109642194A (en) * | 2016-07-01 | 2019-04-16 | 广岛县 | Melting with utensil and melt method by the reproduction cell of glassy state storage |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5739955A (en) * | 1980-08-22 | 1982-03-05 | Mitsubishi Heavy Ind Ltd | Heat insulating material which cause no thermal curving |
| JPH03140784A (en) * | 1989-10-25 | 1991-06-14 | Hitachi Ltd | Box body for refrigerator and manufacture of the same |
| CN202594113U (en) * | 2012-05-30 | 2012-12-12 | 广州赛能冷藏科技有限公司 | Heat-preservation deep freezer |
| CN204426477U (en) * | 2015-01-23 | 2015-07-01 | 马建波 | A kind of Simple cell program freezing storing box |
-
2016
- 2016-01-19 CN CN201610032792.XA patent/CN105475269B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5739955A (en) * | 1980-08-22 | 1982-03-05 | Mitsubishi Heavy Ind Ltd | Heat insulating material which cause no thermal curving |
| JPH03140784A (en) * | 1989-10-25 | 1991-06-14 | Hitachi Ltd | Box body for refrigerator and manufacture of the same |
| CN202594113U (en) * | 2012-05-30 | 2012-12-12 | 广州赛能冷藏科技有限公司 | Heat-preservation deep freezer |
| CN204426477U (en) * | 2015-01-23 | 2015-07-01 | 马建波 | A kind of Simple cell program freezing storing box |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109642194A (en) * | 2016-07-01 | 2019-04-16 | 广岛县 | Melting with utensil and melt method by the reproduction cell of glassy state storage |
| CN107629941A (en) * | 2017-10-27 | 2018-01-26 | 中国科学院理化技术研究所 | A kind of cell cryopreservation device and its application based on micro-fluidic chip |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105475269B (en) | 2018-04-17 |
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Address after: 646000 Taiping Street, Sichuan, Luzhou, No. 25 Applicant after: THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIVERSITY Address before: 646000 Taiping Street, Sichuan, Luzhou, No. 25 Applicant before: Hospital Attached to Luzhou Medical College |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180417 Termination date: 20210119 |
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| CF01 | Termination of patent right due to non-payment of annual fee |