CN105439814A - Method for preparing D-mannoheptulose and persitol by extraction from avocado - Google Patents

Method for preparing D-mannoheptulose and persitol by extraction from avocado Download PDF

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CN105439814A
CN105439814A CN201510905721.1A CN201510905721A CN105439814A CN 105439814 A CN105439814 A CN 105439814A CN 201510905721 A CN201510905721 A CN 201510905721A CN 105439814 A CN105439814 A CN 105439814A
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exchange resin
elutriant
resin
ion exchange
persitol
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潘争红
宁德生
黄思思
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Guangxi Institute of Botany of CAS
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    • C07—ORGANIC CHEMISTRY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
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Abstract

The invention discloses a method for preparing high-purity D-mannoheptulose and persitol by extraction from avocado. The method comprises the step of separation and purification by loading an extracting solution of avocado into ion exchange resin. The ion exchange resin is calcium ion-exchange resin, specifically Amberlite CR1320Ca ion exchange resin, DTF-01 calcium ion-exchange resin, Diaion UBK-555 Ca calcium ion-exchange resin, PCR642Ca ion exchange resin or ZG SPC 106 Ca storng acid cation exchange resin. During elution, water is used as an eluent; fractional collection of eluants is carried out; an eluant rich in D-mannoheptulose and an eluant rich in persitol are respectively merged respectively; and alcohol precipitation is carried out respectively to obtain a D-mannoheptulose product and a persitol product respectively. The method of the invention is simple and easy to operate, production cycle is short, and purity of the products obtained is high.

Description

The method preparing D-mannoheptulose and persitol is extracted from avocado
Technical field
The present invention relates to the preparation method of D-mannoheptulose and persitol, be specifically related to from avocado, extract the method preparing D-mannoheptulose and persitol.
Background technology
Avocado (Perseaamericana) has another name called shea, happiness fruit etc., is a kind of emerging torrid zone, subtropics name fruit, originates in Central America, at present with southern US, Guatemala, Mexico and Cuba's cultivation at most.Avocado was introduced in 1918 in China Taiwan first, nineteen twenty-five has plantation in the ground such as Guangzhou, Guangdong Province, Shantou, Jieyang interiorly, has extended to Hainan, Guangdong, Guangxi, Fujian, Yunnan, Sichuan, Zhejiang, Guizhou, Hunan etc. so far and has economized (district).The lipid acid of needed by human, protein, mineral substance and various VITAMIN is rich in avocado meat, the good reputation of " Source of life " is enjoyed in Central America (country of origin), meet the requirement of human consumer to health fruit, the domestic consumption to avocado also grows with each passing day at present.
Current avocado utilize market mainly to sell as fruit and for the extraction of Lipoval A, and the waste pericarp produced after utilizing, fruit stone are (larger, account for fresh fruit heavy 13%) and pomace be commercially exploited not yet, and there is potential Development volue in the position such as the branches and leaves of avocado, root, skin too.Research shows, the plant chemical ingredient of various structures type is there is in avocado, comprise volatile oil, terpene, flavones, alkaloid, steroidal, carotenoid, longer chain fatty acid alcohol derivate etc., and antiviral, antimycotic, cytotoxicity, anti-oxidant, to protect the liver etc. in show good biological activity.The 7-carbon sugar (alcohol) such as the more rare D-mannoheptulose of occurring in nature and persitol are also contained in avocado, they all have good biological function: suppress glucokinase as D-mannoheptulose has and reduce the functions such as insulin level, persitol then has antibacterial and antianaphylaxis effect preferably, the composition of D-mannoheptulose and persitol by increasing antibacterial peptide, can optimize the generation of hBD-2 and incite inflammation or anaphylaxis are not treated and prevented congenital or acquired immunity variation disease.
The people such as MarjolaineD.Meyer carry out com-parison and analysis (Developmentofarapidmethodforthesequentialextractionandsu bsequentquantificationoffattyacidsandsugarsfromavocadome socarptissue to the carbohydrate content extracting method in avocado mesocarp, DOI:10.1021/jf8011322), confirm that D-mannoheptulose and persitol are its main sugars.At present, about the separation purifying technique research report of avocado C7 carbohydrate is less, the people such as NordalA are only had to isolate D-mannoheptulose by the method for ion exchange resin and repeatedly alcohol precipitation from the extracting solution of avocado leaves and persitol (Isolationofmannoheptuloseandidentificationofitsphosphate inavocadoleaves) testing sequence is as follows: to get fresh avocado leaf cold water lixiviate three times, filter, filtrate is centrifugal after being heated to 80 ~ 90 DEG C of maintenance 1h obtains supernatant liquor, through Dowex-50 and DuoliteA3 ion-exchange resin decolorization and desalination after supernatant concentration, resin flow fluid is condensed into rare syrup at not higher than 40 DEG C of temperature, then the extraction using alcohol 2 times of 60% of boiling is added after a large amount of precipitation removed by the ethanol adding its 2 times of volumes 70 DEG C again, ethanol extract containing persitol is condensed into dense syrup, add the methyl alcohol boiled under the condition of 10 DEG C, to place a couple of days obtain thick perseitol crystallization, then 80% dissolve with methanol of a small amount of heat is used, recrystallization after activated carbon decolorizing, dilute with water after the disposing mother liquor ethanol of extraction perseitol, add bread yeast, at 37 DEG C of bottom fermentation 24h, filtering fermentation liquor, after concentrated again in the same way, repeat fermentation three times, fermented liquid adds the hot methanol of 4 times of volumes after being condensed into dense syrup, obtain thick D-mannoheptulose after cooling, use 85% recrystallizing methanol.But the technique of the method is comparatively loaded down with trivial details and the production cycle is longer, purity cannot ensure.In the fresh leaf of yield inside document: 18kg, both yields are D-mannoheptulose 160g, persitol 55g respectively.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of extraction from avocado and prepares the method for D-mannoheptulose and persitol.The method technique is simple to operation, with short production cycle, and D-mannoheptulose product moderate purity >=70% of gained, gained persitol product moderate purity >=90%.
For solving the problems of the technologies described above, the present invention by the following technical solutions:
The method preparing D-mannoheptulose and persitol is extracted from avocado, comprise the step extracting solution of avocado being crossed ion exchange resin separation and purification, it is characterized in that: described ion exchange resin is calcium type ion exchange resin, be specially AmberliteCR1320Ca ion exchange resin, DTF-01 calcium type ion exchange resin, DiaionUBK-555Ca ion exchange resin, pCR642Ca ion exchange resin or ZGSPC106Ca storng-acid cation exchange resin; Be eluent with water during wash-out, Fractional Collections elutriant, merges respectively and is rich in the elutriant of D-mannoheptulose and is rich in the elutriant of persitol, carry out alcohol precipitation respectively, obtain D-mannoheptulose product and persitol product respectively.
The present inventor finds in long-term experimental study, the extracting solution of above-mentioned calcium type ion exchange resin to avocado is adopted to have extraordinary separation and purification effect, content >=70% of D-mannoheptulose in D-mannoheptulose product obtained as stated above, content >=90% of persitol in gained persitol product.The method technique is simple, with short production cycle, and purity in products obtained therefrom is higher.
In order to improve the purity of D-mannoheptulose and persitol in products obtained therefrom further, before the extracting solution of avocado is crossed calcium type ion exchange resin, preferably also comprise and being fermented by extracting solution, after gained fermented liquid crosses macroporous resin column, washing post, the step of resin anion(R.A) and resin cation (R.C.) purifying crossed successively by gained elutriant.After comprising above-mentioned steps, content >=95% of D-mannoheptulose in gained D-mannoheptulose product, content >=95% of persitol in gained persitol product.
Comprising above-mentioned fermentation, cross macroporous resin column, cross the step of resin anion(R.A) and resin cation (R.C.) purifying successively after, of the present inventionly from avocado, extract the method preparing D-mannoheptulose and persitol, specifically comprise the following steps:
1) extracting solution of avocado is obtained;
2) in extracting solution, add yeast to ferment, gained fermented liquid is centrifugal, collects supernatant liquor;
3) macroporous resin column crossed by gained supernatant liquor, washing, collects elutriant;
4) gained elutriant crosses resin anion(R.A) and resin cation (R.C.) successively, makes the specific conductivity≤200 μ s/cm of the effluent liquid flowed out through resin cation (R.C.), collects the resin cation (R.C.) effluent liquid of specific conductivity≤200 μ s/cm;
5) gained resin cation (R.C.) effluent liquid crosses calcium type ion exchange resin, washing, and Fractional Collections elutriant merges the elutriant being rich in D-mannoheptulose, obtains elutriant A; Merge the elutriant being rich in persitol, obtain elutriant B; Wherein, described calcium type ion exchange resin be AmberliteCR1320Ca ion exchange resin, DTF-01 calcium type ion exchange resin, DiaionUBK-555Ca ion exchange resin, pCR642Ca ion exchange resin, ZGSPC106Ca storng-acid cation exchange resin;
6) elutriant A is concentrated after decolouring, obtain concentrated solution A, add ethanol or methyl alcohol alcohol precipitation, isolate crystal, dry, obtain the D-mannoheptulose of content >=95%; Elutriant B is concentrated, obtains concentrated solution B, add ethanol or methyl alcohol alcohol precipitation, isolate crystal, dry, obtain the persitol of content >=95%.
In technique scheme, in extracting solution, add yeast can remove starch in extracting solution or other glucide.When the volume of extracting solution is comparatively huge, can first to extracting solution carry out concentrated after add yeast again and ferment.The applicant finds in the implementation, and when the solid content that extracting solution concentrates in the concentrated solution of gained is 3 ~ 10%, the add-on of yeast is 1 ~ 2 ‰ of concentrated solution weight, can remove the starch in extracting solution or other glucide so preferably.When carrying out under 15 ~ 35 DEG C of conditions when fermenting, the time of fermentation is generally 24 ~ 48h.
The step 2 of aforesaid method) in, described yeast can be one or more the combination be selected from bread yeast, high activity dried yeast, yeast saccharomyces cerevisiae and fruit wine yeast.
The step 3 of aforesaid method) in, the model of macroporous resin is same as the prior art, can be preferably D101, HPD-400, XDA-5, LSA-20, AB-8, NKA or D4020.
The step 4 of aforesaid method) in, described resin anion(R.A) can be 201 × 4,202, D290 or D280 resin anion(R.A); Described resin cation (R.C.) can be 001 × 7,001 × 8, D001, C100EDL or S108 resin cation (R.C.).
The step 4 of aforesaid method) in, step 3) gained elutriant is after crossing resin anion(R.A) and resin cation (R.C.) successively, and the specific conductivity of the effluent liquid flowed out through resin cation (R.C.) can reach usually≤requirement of 200 μ s/cm; Specific conductivity as the effluent liquid crossing resin anion(R.A) and resin cation (R.C.) is successively greater than 200 μ s/cm, then can regulate by reducing applied sample amount, increase resin bed volume or the flow velocity reduced through resin anion(R.A) and resin cation (R.C.), reaching above-mentioned requirements to make the specific conductivity of effluent liquid.
The step 1 of aforesaid method) in, the extracting solution of described avocado can extract avocado by existing routine techniques and obtain.Preferably, adopt with the fruit of avocado (comprising pericarp, pulp and fruit stone), stem, branches and leaves for raw material, extract for solvent with water or containing the low-carbon alcohol of 1 ~ 4 carbon atom, thus obtain the extracting solution of avocado.The mode wherein extracted can be lixiviate, supersound extraction, refluxing extraction etc., the number of times extracted is generally 1 ~ 3 time, during each extraction, the add-on of solvent is generally 2 ~ 8 times of raw material weight, the time of each extraction is generally 30 ~ 120min, and during extraction, the volumetric concentration of low-carbon alcohol used is preferably 5 ~ 95%.
The step 5 of aforesaid method) in, alcohol precipitation operation is same as the prior art, normally crystallization under-5 ~ 25 DEG C of conditions after adding ethanol.
The step 6 of aforesaid method) in, the decolouring of elutriant A can adopt existing routine techniques to realize, can be specifically realize by elutriant A being crossed D730, D750, D354FD or SD300 decolorizing resin post, also can use activated carbon decolorizing, can also be the combination adopting above-mentioned two or more mode.
Compared with prior art, feature of the present invention is:
1, the present invention by avocado merely through a Ca 2+namely type ion exchange resin realize being separated of D-mannoheptulose and persitol, and obtain the D-mannoheptulose of purity>=70% and the persitol of purity>=90% by alcohol precipitation, and technique is simple to operation, with short production cycle, is easy to industrialization.
2, further, the present invention comprising fermentation, after macroporous resin column crossed by gained fermented liquid, the step of resin anion(R.A) and resin cation (R.C.) purifying crossed successively by gained elutriant after washing post, then in conjunction with Ca 2+type ion-exchange resin purification, can obtain highly purified product, wherein content>=95% of D-mannoheptulose in gained D-mannoheptulose product, content>=95% of persitol in gained persitol product.
3, the present invention further expands the stem of avocado, leaf all can be used for extracting, and using the original material as refuse process as raw material, not only improves the commercial value of avocado, and reduces the production cost of product.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and to understand content of the present invention better, but the present invention is not limited to following examples.
Embodiment 1
1) get the forced air drying in 50 DEG C of baking ovens of avocado fruit, be ground into 30 ~ 40 orders, obtain avocado powder (detect through HPLC, wherein the content of D-mannoheptulose is 0.41%, and the content of persitol is 0.56%), for subsequent use;
2) get avocado powder 300g and be placed in extraction vessel, add 10% ethanol 600mL supersound extraction 30min wherein, filter, filter residue adds 10% ethanol 600mL supersound extraction 30min again, and merging filtrate, obtains extracting solution;
3) extracting solution is evaporated to solid content is 6%, and in gained concentrated solution, add Angel Yeast powder, normal temperature bottom fermentation 24h, the add-on of described bread yeast is 1 ‰ of concentrated solution weight;
4) gained fermented liquid is centrifugal, collects supernatant liquor and crosses D101 macroporous resin column, use deionized water wash-out, collect elutriant;
5) gained elutriant crosses 001 × 8 resin cation (R.C.) (post bed: Φ 35mm × 600mm) and 201 × 4 resin anion(R.A)s (post bed: Φ 35mm × 600mm) desalination bleaching successively with the flow velocity of 2mL/min, the specific conductivity of effluent liquid flowed out from resin anion(R.A) is 175 μ s/cm, collects whole resin anion(R.A) effluent liquid;
6) step 5) the resin anion(R.A) effluent liquid collected is condensed into the syrup that solid content is 25%, cross AmberliteCR1320Ca ion exchange resin (ROHM AND HAAS resin Chinese companies), deionized water wash-out, Fractional Collections elutriant, after HPLC analyzes, merge the elutriant being rich in D-mannoheptulose, obtain elutriant A; Merge the elutriant being rich in persitol, obtain elutriant B;
7) elutriant A is concentrated after D730 decolouring, obtain concentrated solution A, add the 95v/v% ethanol being equivalent to its volume 2 times wherein, be placed in crystallization under 5 DEG C of conditions, isolate crystal, dry, obtain the D-mannoheptulose 0.9g that content is 95.6%; Elutriant B is concentrated, obtains concentrated solution B, add the 95v/v% ethanol being equivalent to its volume 2 times wherein, be placed in crystallization under 5 DEG C of conditions, isolate crystal, dry, obtain the persitol 1.4g that content is 97.2%.
Embodiment 2
1) adopt the raw material identical with embodiment 1 and press the extracting solution that the identical method of embodiment 1 obtains avocado;
2) mistake after gained extracting solution recovery ethanol pCR642Ca ion exchange resin (Piao Laite company), deionized water wash-out, Fractional Collections elutriant, after HPLC analyzes, merges the elutriant being rich in D-mannoheptulose, obtains elutriant A; Merge the elutriant being rich in persitol, obtain elutriant B;
3) elutriant A is concentrated after activated carbon decolorizing, obtain concentrated solution A, add the 95v/v% ethanol being equivalent to its volume 3 times wherein, be placed in crystallization under 15 DEG C of conditions, isolate crystal, dry, obtain the D-mannoheptulose 1.3g that content is 71.2%; Elutriant B is concentrated, obtains concentrated solution B, add the 95v/v% ethanol being equivalent to its volume 2 times wherein, be placed in crystallization under 15 DEG C of conditions, isolate crystal, dry, obtain the persitol 1.5g that content is 90.4%.
Embodiment 3
1) get avocado fruit through normal freeze-drying, be ground into 30 ~ 40 orders, obtain avocado powder (detect through HPLC, wherein the content of D-mannoheptulose is 0.4%, and the content of persitol is 0.52%), for subsequent use;
2) get avocado powder 500g and be placed in extraction vessel, add the ethanol 2000mL supersound extraction 30min that volumetric concentration is 50% wherein, filter, filter residue adds the ethanol 2000mL supersound extraction 30min that volumetric concentration is 50% again, merging filtrate, obtains extracting solution;
3) extracting solution is evaporated to solid content is 8%, and in gained concentrated solution, add Angel fruit wine yeast, normal temperature bottom fermentation 24h, the add-on of described fruit wine yeast is 1.5 ‰ of concentrated solution weight;
4) gained fermented liquid is centrifugal, collects supernatant liquor and crosses HPD-400 macroporous resin column, use deionized water wash-out, collect elutriant;
5) gained elutriant crosses 001 × 7 resin cation (R.C.) (post bed: Φ 45mm × 600mm) and 202 resin anion(R.A)s (post bed: Φ 45mm × 600mm) desalination bleaching successively with the flow velocity of 2mL/min, the specific conductivity of effluent liquid flowed out from resin anion(R.A) is 190 μ s/cm, collects whole resin anion(R.A) effluent liquid;
6) step 5) to be condensed into solid content be 28% syrup for the resin anion(R.A) effluent liquid collected, cross DTF-01 calcium type ion exchange resin (Jiangsu Suqing Water Treatment Engineering Group Co., Ltd.), deionized water wash-out, Fractional Collections elutriant, after HPLC analyzes, merge the elutriant being rich in D-mannoheptulose, obtain elutriant A; Merge the elutriant being rich in persitol, obtain elutriant B;
7) elutriant A is concentrated after D750 decolouring, obtain concentrated solution A, add the methyl alcohol being equivalent to its volume 3 times wherein, be placed in crystallization under 15 DEG C of conditions, isolate crystal, dry, obtain the D-mannoheptulose 1.5g that content is 95%; Elutriant B is concentrated, obtains concentrated solution B, add the methyl alcohol being equivalent to its volume 2 times wherein, be placed in crystallization under 15 DEG C of conditions, isolate crystal, dry, obtain the persitol 2.1g that content is 98.1%.
Embodiment 4
1) adopt the raw material identical with embodiment 1 and press the extracting solution that the identical method of embodiment 1 obtains avocado;
2) DTF-01 calcium type ion exchange resin crossed by gained extracting solution after reclaiming ethanol, deionized water wash-out, and Fractional Collections elutriant, after HPLC analyzes, merges the elutriant being rich in D-mannoheptulose, obtains elutriant A; Merge the elutriant being rich in persitol, obtain elutriant B;
3) elutriant A is concentrated after D750 decolouring, obtain concentrated solution A, add the dehydrated alcohol being equivalent to its volume 2 times wherein, be placed in crystallization under 15 DEG C of conditions, isolate crystal, dry, obtain the D-mannoheptulose 2.2g that content is 70.4%; Elutriant B is concentrated, obtains concentrated solution B, add its isopyknic dehydrated alcohol wherein, be placed in crystallization under 15 DEG C of conditions, isolate crystal, dry, obtain the persitol 2.4g that content is 91.2%.
Embodiment 5
1) get the forced air drying in 50 DEG C of baking ovens of avocado branches and leaves, be ground into 30 ~ 40 orders, obtain avocado powder (detect through HPLC, wherein the content of D-mannoheptulose is 0.7%, and the content of persitol is 0.82%), for subsequent use;
2) get avocado powder 500g and be placed in extraction vessel, add pure water 2000mL refluxing extraction 60min wherein, filter, filter residue adds 2000mL pure water refluxing extraction 60min again, and merging filtrate, obtains extracting solution;
3) extracting solution is evaporated to solid content is 3%, in gained concentrated solution, add high activity dried yeast, 30 DEG C of condition bottom fermentation 12h, and the add-on of described high activity dried yeast is 2 ‰ of concentrated solution weight;
4) gained fermented liquid is centrifugal, collects supernatant liquor and crosses AB-8 macroporous resin column, use deionized water wash-out, collect elutriant;
5) gained elutriant crosses S108 resin cation (R.C.) (post bed: Φ 45mm × 600mm) and D280 resin anion(R.A) (post bed: Φ 45mm × 600mm) desalination bleaching successively with the flow velocity of 3mL/min, the specific conductivity of effluent liquid flowed out from resin anion(R.A) is 170 μ s/cm, collects whole resin anion(R.A) effluent liquid;
6) step 5) the resin anion(R.A) effluent liquid collected is condensed into the syrup that solid content is 30%, cross ZGSPC106Ca ion exchange resin (Beijing win honour for foundation Science and Technology Ltd.), deionized water wash-out, Fractional Collections elutriant, after HPLC analyzes, merge the elutriant being rich in D-mannoheptulose, obtain elutriant A; Merge the elutriant being rich in persitol, obtain elutriant B;
7) elutriant A is concentrated after D354FD decolorizing resin and activated carbon decolorizing, obtain concentrated solution A, add the methyl alcohol being equivalent to its volume 2 times wherein, be placed in crystallization under 0 DEG C of condition, isolate crystal, dry, obtain the D-mannoheptulose 2.9g that content is 96.2%; Elutriant B is concentrated, obtains concentrated solution B, add isopyknic methyl alcohol wherein, be placed in crystallization under 0 DEG C of condition, isolate crystal, dry, obtain the persitol 3.5g that content is 98%.
Embodiment 6
Repeat embodiment 1, replace AmberliteCR1320Ca ion exchange resin unlike with DiaionUBK-555Ca ion exchange resin (Mitsubishi chemical Co., Ltd).
Result is: obtain the D-mannoheptulose 0.84g that content is 95.1%, obtains the persitol 1.2g that content is 97.7%.

Claims (10)

1. from avocado, extract the method preparing D-mannoheptulose and persitol, comprise the step extracting solution of avocado being crossed ion exchange resin separation and purification, it is characterized in that: described ion exchange resin is calcium type ion exchange resin, be specially AmberliteCR1320Ca ion exchange resin, DTF-01 calcium type ion exchange resin, DiaionUBK-555Ca ion exchange resin, pCR642Ca ion exchange resin or ZGSPC106Ca storng-acid cation exchange resin; Be eluent with water during wash-out, Fractional Collections elutriant, merges respectively and is rich in the elutriant of D-mannoheptulose and is rich in the elutriant of persitol, carry out alcohol precipitation respectively, obtain D-mannoheptulose product and persitol product respectively.
2. method according to claim 1, it is characterized in that: the extracting solution of described avocado is before crossing calcium type ion exchange resin, also comprise and being fermented by extracting solution, after gained fermented liquid crosses macroporous resin column, washing post, the step of resin anion(R.A) and resin cation (R.C.) purifying crossed successively by gained elutriant.
3. method according to claim 1 and 2, is characterized in that: specifically comprise the following steps:
1) extracting solution of avocado is obtained;
2) in extracting solution, add yeast to ferment, gained fermented liquid is centrifugal, collects supernatant liquor;
3) macroporous resin column crossed by gained supernatant liquor, washing, collects elutriant;
4) gained elutriant crosses resin anion(R.A) and resin cation (R.C.) successively, makes the specific conductivity≤200 μ s/cm of the effluent liquid flowed out through resin cation (R.C.), collects the resin cation (R.C.) effluent liquid of specific conductivity≤200 μ s/cm;
5) gained resin cation (R.C.) effluent liquid crosses calcium type ion exchange resin, washing, and Fractional Collections elutriant merges the elutriant being rich in D-mannoheptulose, obtains elutriant A; Merge the elutriant being rich in persitol, obtain elutriant B; Wherein, described calcium type ion exchange resin be AmberliteCR1320Ca, DTF-01 calcium type ion exchange resin, DiaionUBK-555Ca ion exchange resin, pCR642Ca ion exchange resin, ZGSPC106Ca storng-acid cation exchange resin;
6) elutriant A is concentrated after decolouring, obtain concentrated solution A, add ethanol or methyl alcohol alcohol precipitation, isolate crystal, dry, obtain the D-mannoheptulose product of content >=95%; Elutriant B is concentrated, obtains concentrated solution B, add ethanol or methyl alcohol alcohol precipitation, isolate crystal, dry, obtain the persitol product of content >=95%.
4. method according to claim 3, is characterized in that: step 2) in, add yeast again after extracting solution is concentrated and ferment.
5. method according to claim 4, is characterized in that: when the solid content in concentrated solution is 3 ~ 10%, and the add-on of yeast is 1 ~ 2 ‰ of concentrated solution weight.
6. method according to claim 3, is characterized in that: step 2) in, described yeast is one or more the combination be selected from bread yeast, high activity dried yeast, yeast saccharomyces cerevisiae and fruit wine yeast.
7. method according to claim 3, is characterized in that: step 3) in, the model of macroporous resin is D101, HPD-400, XDA-5, LSA-20, AB-8, NKA or D4020.
8. method according to claim 3, is characterized in that: step 4) in, described resin anion(R.A) is 201 × 4,202, D290 or D280 resin anion(R.A).
9. method according to claim 3, is characterized in that: step 4) in, described resin cation (R.C.) is 001 × 7,001 × 8, D001, C100EDL or S108 resin cation (R.C.).
10. method according to claim 3, is characterized in that: step 1) in, with the fruit of avocado, stem, branches and leaves, root or skin for raw material, extract for solvent with water or containing the low-carbon alcohol of 1 ~ 4 carbon atom, obtain the extracting solution of avocado.
CN201510905721.1A 2015-12-09 2015-12-09 Method for preparing D-mannoheptulose and persitol by extraction from avocado Pending CN105439814A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1968707A (en) * 2004-04-30 2007-05-23 科学发展实验室 Use of a compound comprising D-mannoheptulose and/or perseitol for treating and preventing innate immunity modification diseases
CN101497903A (en) * 2008-02-01 2009-08-05 唐传生物科技（厦门）有限公司 Method for selectively converting and shunting biological products
FR3001889A1 (en) * 2013-02-11 2014-08-15 Expanscience Lab USE OF A COMPOSITION COMPRISING AVOCADO PERSEOSE IN THE PROTECTION OF EPIDERMAL STEM CELLS.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1968707A (en) * 2004-04-30 2007-05-23 科学发展实验室 Use of a compound comprising D-mannoheptulose and/or perseitol for treating and preventing innate immunity modification diseases
CN101497903A (en) * 2008-02-01 2009-08-05 唐传生物科技（厦门）有限公司 Method for selectively converting and shunting biological products
FR3001889A1 (en) * 2013-02-11 2014-08-15 Expanscience Lab USE OF A COMPOSITION COMPRISING AVOCADO PERSEOSE IN THE PROTECTION OF EPIDERMAL STEM CELLS.

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