CN105434518A - Traditional Chinese medicine preparation for treating hepatitis - Google Patents
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Abstract
The invention discloses a traditional Chinese medicine preparation for treating hepatitis. With radix sophorae tonkinensis as a raw medicinal material, precipitation and purification are carried out with high-concentration ethanol after ethanol extraction; high-purity active components are prepared by a macroporous resin-polyamide resin and cation exchange resin joint separation technology; and a clinically acceptable preparation is prepared. According to the physical and chemical properties of the active components of the radix sophorae tonkinensis, high-purity flavone and alkaloid active components are separated by the modern chromatographic macroporous resin-polyamide resin and cation exchange resin joint separation technology; the purity can reach over 90% through liquid mass detection; and the traditional Chinese medicine preparation is few in impurity, relatively high in clinical activity, low in adverse reaction, and better in safety in comparison with the prior art.
Description
Technical field
The present invention relates to a kind of medicine, be specifically related to a kind of Chinese medicine preparation for the treatment of hepatitis and preparation method thereof.
Background technology
China is one of world's viral hepatitis state occurred frequently, and annual neopathy about has more than 200 ten thousand examples, there is no fully effective treatment means and specific drug at present, the treatment such as interferon hepatitis clinically, but interferons drug effect is limited, and also side effect is many, expensive, be difficult to large-scale popularization and use.
Radix Sophorae Tonkinensis is bitter, cold; Poisonous, have heat-clearing and toxic substances removing, detumescence sore-throat relieving is efficient, is used for the treatment of fire-toxin clinically and pents up, laryngopharynx swelling and pain, gingivitis, aphtha of the mouth and tongue and hepatitis.Modern study shows, Radix Sophorae Tonkinensis contains the alkaloids such as matrine and flavone compound.
Existing Radix Sophorae Tonkinensis Chinese medicine preparation, extracting method is simple, especially can not according to the active component of Radix Sophorae Tonkinensis, the extraction and isolation of science, causes effective ingredient to run off, impurity is more, cost is high, inconvenient operation, clinically poor effect, and there is larger untoward reaction, greatly limit the clinical practice of Radix Sophorae Tonkinensis Chinese medicine preparation.
Summary of the invention
Goal of the invention: the object of the invention is to solve the deficiencies in the prior art, combine with Pharmacological Activity Screening and modern process for separation and purification, provide a kind of extracts active ingredients efficiency high, the Chinese medicine preparation of easy to operate treatment hepatitis.
Technical scheme: in order to realize above object, the technical scheme that the present invention takes is:
Treat a Chinese medicine preparation for hepatitis, it is prepared by following method:
(1) get Radix Sophorae Tonkinensis medical material, add ethanol extraction, concentrated, obtain ethanol extract, add ethanol, make concentration of alcohol be 80 ~ 90%, staticly settle, filter, get filtrate, concentrated, obtain concentrated solution;
(2) get the concentrated solution that step (1) prepares, upper macroporous adsorptive resins, first washes with water, then uses 10% successively, the ethanol elution of 20%, 30%, 40%, 50% and 60%, collect the ethanol elution of 40% and 50%, merge, concentrate to obtain macroporous resin eluate;
(3) get the macroporous resin eluate that step (2) obtains, upper polyamide resin column, uses 10% successively, and 20%, 30%, 40%, 50%, 60%, the ethanol elution of 70% and 80%, collects 50% and 60% ethanol elution, concentrates to obtain polyamide eluate;
(4) get the ethanol elution of step (3) 70% and 80%, concentrated, concentrated solution adds hydrochloric acid, adjust pH is 1 ~ 3, then goes up cation exchange resin column, uses water and ammonia spirit eluting successively, obtain ammonia eluent, concentrated, obtain cation exchange resin eluate;
(5) get step (3) polyamide eluate and step (4) cation exchange resin eluate, add pharmaceutically acceptable carrier, prepare injection, tablet, granule, capsule or oral liquid.
Preferably, the Chinese medicine preparation of above-described treatment hepatitis, adds the ethanol extraction of Radix Sophorae Tonkinensis medical material weight 8 ~ 15 times of volumes in step (1), extracting method is percolation, reflux extraction or continuous circumfluence extraction.
Preferably, the Chinese medicine preparation of above-described treatment hepatitis, in step (1), extraction time is 1 ~ 3 time, and extraction time is 0.5 ~ 2 hour.
Preferably, the Chinese medicine preparation of above-described treatment hepatitis, the macroporous adsorbent resin described in step (2) is D101, D101B, XDA-1 or H-60D type.
Preferably, the Chinese medicine preparation of above-described treatment hepatitis, the cation exchange resin described in step (4) is storng-acid cation exchange resin.
Preferably, the Chinese medicine preparation of above-described treatment hepatitis, step (3) polyamide eluate comprises the flavone compound that weight percentage is 50 ~ 95%; Step (4) cation exchange resin eluate comprises the alkaloid compound that weight percentage is 55 ~ 98%.
Preferably, the Chinese medicine preparation of above-described treatment hepatitis, flavone compound comprises d-maackianin-β-D-glucoside, pterocarpin and trifolirhizin.
Preferably, the Chinese medicine preparation of above-described treatment hepatitis, it is characterized in that, alkaloid compound comprises matrine, oxymatrine and sophocarpine, sophoridine.
When the present invention is made oral liquid, each extract adding distil water is dissolved, filter, add syrup, regulate pH, make oral liquid.
When the present invention is made tablet, each extract and lactose or corn starch, add magnesium stearate lubricant when needing, mix homogeneously, granulate, then tablet made by tabletting.
When the present invention makes capsule, each extract and carrier lactose or corn starch mix homogeneously, granulate, then encapsulatedly makes capsule.
When the present invention makes granule, extract and diluent lactose or corn starch mix homogeneously, granulate, dry, make granule.
When the present invention makes injection, each extract is added physiological saline solution and then adds activated carbon, stir, 80 DEG C are heated 30 minutes, filter, adjust ph, with sintered glass funnel or other frit to clear and bright, fill, makes injection in 30 minutes 100 to 115 DEG C of sterilizings.
The present invention is according to the feature of Radix Sophorae Tonkinensis active component, adopt modern separation chromatography scientific combination, first adopt punching resin to live enrichment and obtain alkaloid and flavone compound, then adopt polyamide resin column to be separated and obtain flavonoid active component (comprising d-maackianin-β-D-glucoside, pterocarpin and trifolirhizin etc.), and then obtain matrine with cation exchange resin column separation, oxymatrine, sophocarpine, the alkaloids active component such as sophoridine, the present invention prepares at Radix Sophorae Tonkinensis extract, active component purity is high, impurity is little, thus untoward reaction is little, safety is high, the shortcoming that prior art Radix Sophorae Tonkinensis preparation toxic and side effects is large can be overcome, show through functional experiment research, Radix Sophorae Tonkinensis Chinese medicine preparation provided by the invention, effectively can improve liver function, the liver function resisted caused by various inflammation declines, control to reduce bilirubin abnormal, hepatocyte is promoted to repair and regeneration, to hepatitis, there is well treatment efficient.
Beneficial effect: the present invention is according to the kind of Radix Sophorae Tonkinensis active component and physicochemical property, adopt modern chromatographic macroporous resin-polyamide and cation exchange resin combined separation technology, be separated and obtain the high flavonoid of purity and alkaloids active component, can up to more than 90% through liquid quality detection purity, impurity is few, activity is stronger clinically, and have very low untoward reaction, safety is better compared to existing technology.
Detailed description of the invention
The present invention is illustrated further below in conjunction with specific embodiment, these embodiments should be understood only be not used in for illustration of the present invention and limit the scope of the invention, after having read the present invention, the amendment of those skilled in the art to the various equivalent form of value of the present invention has all fallen within the application's claims limited range.
Embodiment 1:
1. treat a Chinese medicine preparation for hepatitis, it is prepared by following method:
(1) get Radix Sophorae Tonkinensis medical material, add 60% alcohol reflux 2 times of 10 times of volumes, each 2 hours, merge extractive liquid, concentrated, obtain ethanol extract, add ethanol, make concentration of alcohol be 80%, staticly settle, filter, get filtrate, concentrated, obtain concentrated solution;
(2) concentrated solution that step (1) prepares is got, upper D101 macroporous adsorptive resins, first wash with water, then use 10% successively, 20%, 30%, the ethanol elution of 40%, 50% and 60%, collects the ethanol elution of 40% and 50%, merge, concentrate to obtain macroporous resin eluate;
(3) get the macroporous resin eluate that step (2) obtains, upper polyamide resin column, uses 10% successively, and 20%, 30%, 40%, 50%, 60%, the ethanol elution of 70% and 80%, collects 50% and 60% ethanol elution, concentrates to obtain polyamide eluate;
(4) get the ethanol elution of step (3) 70% and 80%, concentrated, concentrated solution adds hydrochloric acid, adjust pH is 2, then goes up strong acid cation exchange resin column, uses water and ammonia spirit eluting successively, obtain ammonia eluent, concentrated, obtain cation exchange resin eluate;
(5) step (3) polyamide eluate (flavone compound) and step (4) cation exchange resin eluate (alkaloid compound) is got, adding distil water dissolves, filter, add syrup, regulate pH to 7.5, oral liquid, embedding subpackage and get final product.
Embodiment 2
1. treat a Chinese medicine preparation for hepatitis, it is prepared by following method:
(1) get Radix Sophorae Tonkinensis medical material, 70% ethanol percolation adding 12 times of volumes extracts 3 times, merge extractive liquid, concentrated, obtains ethanol extract, adds ethanol, make concentration of alcohol be 85%, staticly settle, and filters, gets filtrate, concentrated, obtains concentrated solution;
(2) concentrated solution that step (1) prepares is got, upper H-60D macroporous adsorptive resins, first wash with water, then use 10% successively, 20%, 30%, the ethanol elution of 40%, 50% and 60%, collects the ethanol elution of 40% and 50%, merge, concentrate to obtain macroporous resin eluate;
(3) get the macroporous resin eluate that step (2) obtains, upper polyamide resin column, uses 10% successively, and 20%, 30%, 40%, 50%, 60%, the ethanol elution of 70% and 80%, collects 50% and 60% ethanol elution, concentrates to obtain polyamide eluate;
(4) get the ethanol elution of step (3) 70% and 80%, concentrated, concentrated solution adds hydrochloric acid, adjust pH is 3, then goes up strong acid cation exchange resin column, uses water and ammonia spirit eluting successively, obtain ammonia eluent, concentrated, obtain cation exchange resin eluate;
(5) step (3) polyamide eluate (flavone compound) and step (4) cation exchange resin eluate (alkaloid compound) is got, add appropriate Icing Sugar and stabilizing agent, cross 40 mesh sieves, granulate, obtain granule, subpackage every bag 10g and get final product.
Embodiment 3
1. treat a Chinese medicine preparation for hepatitis, it is prepared by following method:
(1) get Radix Sophorae Tonkinensis medical material, add 70% alcohol reflux 2 times of 15 times of volumes, each 1.5 hours, merge extractive liquid, concentrated, obtain ethanol extract, add ethanol, make concentration of alcohol be 80%, staticly settle, filter, get filtrate, concentrated, obtain concentrated solution;
(2) concentrated solution that step (1) prepares is got, upper XDA-1 macroporous adsorptive resins, first wash with water, then use 10% successively, 20%, 30%, the ethanol elution of 40%, 50% and 60%, collects the ethanol elution of 40% and 50%, merge, concentrate to obtain macroporous resin eluate;
(3) get the macroporous resin eluate that step (2) obtains, upper polyamide resin column, uses 10% successively, and 20%, 30%, 40%, 50%, 60%, the ethanol elution of 70% and 80%, collects 50% and 60% ethanol elution, concentrates to obtain polyamide eluate;
(4) get the ethanol elution of step (3) 70% and 80%, concentrated, concentrated solution adds hydrochloric acid, adjust pH is 3, then goes up strong acid cation exchange resin column, uses water and ammonia spirit eluting successively, obtain ammonia eluent, concentrated, obtain cation exchange resin eluate;
(5) step (3) polyamide eluate (flavone compound) and step (4) cation exchange resin eluate (alkaloid compound) is got, add appropriate Icing Sugar and stabilizing agent, cross 40 mesh sieves, granulate, tabletting, obtained tablet.
Embodiment 4
The polyamide eluate that Example 2 prepares and cation exchange resin eluate, cross 60 mesh sieves, incapsulate, every net weight 0.45g.
Embodiment 5
The polyamide eluate that Example 2 prepares and cation exchange resin eluate, add corn starch, and tablet made by granulate tabletting.
Embodiment 6: pharmacological evaluation 1
Laboratory animal: 60 healthy Kunming mouses, male and female half and half, 15 ~ 25g, is divided into 3 groups of kinds at random by mice: blank group (20), treatment group (20), matched group (20).
Experimental technique: blank group gavage equivalent water for injection every day; The Chinese medicine preparation that the embodiment of the present invention 1 of administration group gavage every day equivalent prepares; Matched group: every day gavage equivalent Radix Sophorae Tonkinensis medicinal substances extract.Its preparation method is: get Radix Sophorae Tonkinensis medical material, pick decontamination, be cut into the section block of 1-3cm, clean by purified water, add in multifunctional extracting pot, add purified water and decoct three times, amount of water is respectively 6 times of medical material amount, 5 times, 5 times, decocting time is 2.0, 1.5, 1.0 hour, collecting decoction, be concentrated into than the 1.20-1.25 that weighs (90 DEG C), ethanol to the alcohol content that concentrated solution slowly adds 95% with vigorous stirring reaches 70-75% (20 DEG C), 0-4 DEG C of cold preservation more than 48 hours, filter, decompression filtrate recycling ethanol also continues to be concentrated into than the 1.20-1.25 that weighs (90 DEG C), concentrated solution slowly adds 95% ethanol to alcohol content with vigorous stirring and reaches 85% (20 DEG C), 0-4 DEG C of cold preservation more than 48 hours, filter, decompression filtrate recycling ethanol is extremely without alcohol taste, concentrated solution adds 3 times amount purified water while stirring, stir, use hydrochloric acid (1:5) adjust pH 2.5 ~ 3.0 again, continue stirring 5 minutes, 0 ~ 4 DEG C of cold preservation pot leaves standstill cold preservation more than 48 hours, it is made to precipitate completely.Filter, filtrate, with about sodium hydroxide adjust ph to 6.5, is evaporated to thick paste (relative density is 1.25 ~ 1.30,50 ~ 55 DEG C of surveys).
Two groups of difference lumbar injection carbon tetrachloride (CCl after 14 days
4), water is can't help in fasting, and 24 as a child, and eyeball gets blood, separation of serum, measures ALT value.Specific experiment result is as shown in table 1:
Table 1 carbon tetrachloride (CCl
4) liver injury model experimental result
| Group | Number of animals | Dosage | ALT value |
| Blank group | 20 | Equivalent water for injection | 136.23±19.56 |
| Treatment group | 20 | 5g (crude drug amount)/kg | 97.14±22.49 |
| Matched group | 20 | 5g (crude drug amount)/kg | 107.57±22.68 |
Embodiment 7: pharmacological evaluation 2
Laboratory animal: 60 healthy Kunming mouses, male and female half and half, 15 ~ 25g, is divided into 2 groups of kinds at random by mice: blank group (20), treatment group (20), matched group (20).
Experimental technique: blank group gavage equivalent water for injection every day; Administration group: every day gavage equivalent the Chinese medicine preparation for preparing of the embodiment of the present invention 1; Matched group: gavage equivalent Radix Sophorae Tonkinensis medicinal substances extract every day (its preparation method is the same).
Two groups of difference lumbar injection thioacetamide (TAA) after 14 days, water is can't help in fasting, and 24 as a child, and eyeball gets blood, separation of serum, measures ALT value.Specific experiment result is as shown in table 2:
Table 2 thioacetamide (TAA) liver injury model experimental result
| Group | Number of animals | Dosage | ALT value |
| Blank group | 20 | Equivalent water for injection | 131.68±30.21 |
| Treatment group | 20 | 5g (crude drug amount)/kg | 96.23±25.21 |
| Matched group | 20 | 5g (crude drug amount)/kg | 117.57±26.74 |
Carbon tetrachloride (CCl
4) and thioacetamide (TAA) be all the conventional tool reagent for acute liver damage modeling, wherein carbon tetrachloride is mainly through internal metabolism and film dissolution, cause hepatocyte injury, index ATL can significantly improve, and thioacetamide RNA interfering is transported thus is affected protein synthesis, cause cell plasma environmental disruption, make hepatic necrosis, index ATL also can significantly improve.Both comparatively conventional medicine Preliminary screening model, the present embodiment 6 and embodiment 7 test show, the Chinese medicine preparation for the treatment of hepatitis provided by the invention can effectively resist carbon tetrachloride (CCl
4) and Experimental injury caused by thioacetamide (TAA), the liver protecting Free-radical ring opening polymerization, reduces serum ALT, alleviates hepatic injury, and is better than the Radix Sophorae Tonkinensis medicinal substances extract that prior art prepares.Show that the present invention has good the liver protecting through the Chinese medicine preparation of the treatment hepatitis preferably prepared and treats the effect of hepatitis.
Embodiment 7: clinical experimental study
Choosing 104 routine chronic hepatitis C cases, take the Chinese medicine preparation that the embodiment of the present invention 2 prepares, 3 months is a course for the treatment of, detects the indexs such as liver function before and after patient consumes, hepatitis C virus serum mark and blood, urine, stool routine, blood urea nitrogen.Clinical test results is as follows:
(1) hepatitis negative conversion rate is 32.25%;
(2) liver function recovery, be significantly improved after the treatment of its leading indicator, ALT recovery rate is 70.15%;
(3) symptom improve, limbs are weak, fullness and oppression in the stomach, lack of appetite poor appetite, feel sick detest oil, body order jaundice improvement effective percentage more than 90%;
(4) to human body main organs and function thereof without obvious toxic-side effects.
Above clinical test results shows: the Chinese medicine preparation that the present invention is prepared by modern science process for separation and purification, there is effects such as improving sings and symptoms, suppression virus replication, recovery liver function, and clinical test results shows not occur untoward reaction, safety is high.
Claims (10)
1. treat a Chinese medicine preparation for hepatitis, it is characterized in that, it is prepared by following method:
(1) get Radix Sophorae Tonkinensis medical material, add ethanol extraction, concentrated, obtain ethanol extract, add ethanol, make concentration of alcohol be 80 ~ 90%, staticly settle, filter, get filtrate, concentrated, obtain concentrated solution;
(2) get the concentrated solution that step (1) prepares, upper macroporous adsorptive resins, first washes with water, then uses 10% successively, the ethanol elution of 20%, 30%, 40%, 50% and 60%, collect the ethanol elution of 40% and 50%, merge, concentrate to obtain macroporous resin eluate;
(3) get the macroporous resin eluate that step (2) obtains, upper polyamide resin column, uses 10% successively, and 20%, 30%, 40%, 50%, 60%, the ethanol elution of 70% and 80%, collects 50% and 60% ethanol elution, concentrates to obtain polyamide eluate;
(4) get the ethanol elution of step (3) 70% and 80%, concentrated, concentrated solution adds hydrochloric acid, adjust pH is 1 ~ 3, then goes up cation exchange resin column, uses water and ammonia spirit eluting successively, obtain ammonia eluent, concentrated, obtain cation exchange resin eluate;
(5) get step (3) polyamide eluate and step (4) cation exchange resin eluate, add pharmaceutically acceptable carrier, prepare injection, tablet, granule, capsule or oral liquid.
2. the Chinese medicine preparation for the treatment of hepatitis according to claim 1, is characterized in that, adds the ethanol extraction of Radix Sophorae Tonkinensis medical material weight 8 ~ 15 times of volumes in step (1), and extracting method is percolation, reflux extraction or continuous circumfluence extraction.
3. the Chinese medicine preparation for the treatment of hepatitis according to claim 2, is characterized in that, in step (1), extraction time is 1 ~ 3 time, and extraction time is 0.5 ~ 2 hour.
4. the Chinese medicine preparation for the treatment of hepatitis according to claim 1 and 2, is characterized in that, the macroporous adsorbent resin described in step (2) is D101, D101B, XDA-1 or H-60D type.
5. the Chinese medicine preparation for the treatment of hepatitis according to claim 1 and 2, is characterized in that, the cation exchange resin described in step (4) is storng-acid cation exchange resin.
6. the Chinese medicine preparation for the treatment of hepatitis according to claim 1, is characterized in that, step (3) polyamide eluate comprises the flavone compound that weight percentage is 50 ~ 95%; Step (4) cation exchange resin eluate comprises the alkaloid compound that weight percentage is 55 ~ 98%.
7. the Chinese medicine preparation for the treatment of hepatitis according to claim 6, is characterized in that, flavone compound comprises d-maackianin-β-D-glucoside, pterocarpin and trifolirhizin.
8. the Chinese medicine preparation for the treatment of hepatitis according to claim 6, it is characterized in that, alkaloid compound comprises matrine, oxymatrine and sophocarpine, sophoridine.
9. treat a Chinese medicine preparation for hepatitis, it is prepared by following method:
(1) get Radix Sophorae Tonkinensis medical material, add 60% alcohol reflux 2 times of 10 times of volumes, each 2 hours, merge extractive liquid, concentrated, obtain ethanol extract, add ethanol, make concentration of alcohol be 80%, staticly settle, filter, get filtrate, concentrated, obtain concentrated solution;
(2) concentrated solution that step (1) prepares is got, upper D101 macroporous adsorptive resins, first wash with water, then use 10% successively, 20%, 30%, the ethanol elution of 40%, 50% and 60%, collects the ethanol elution of 40% and 50%, merge, concentrate to obtain macroporous resin eluate;
(3) get the macroporous resin eluate that step (2) obtains, upper polyamide resin column, uses 10% successively, and 20%, 30%, 40%, 50%, 60%, the ethanol elution of 70% and 80%, collects 50% and 60% ethanol elution, concentrates to obtain polyamide eluate;
(4) get the ethanol elution of step (3) 70% and 80%, concentrated, concentrated solution adds hydrochloric acid, adjust pH is 2, then goes up strong acid cation exchange resin column, uses water and ammonia spirit eluting successively, obtain ammonia eluent, concentrated, obtain cation exchange resin eluate;
(5) get step (3) polyamide eluate and step (4) cation exchange resin eluate, adding distil water dissolves, and filters, adds syrup, regulates pH to 7.5, obtains oral liquid, embedding subpackage and get final product.
10. treat a Chinese medicine preparation for hepatitis, it is prepared by following method:
(1) get Radix Sophorae Tonkinensis medical material, 70% ethanol percolation adding 12 times of volumes extracts 3 times, merge extractive liquid, concentrated, obtains ethanol extract, adds ethanol, make concentration of alcohol be 85%, staticly settle, and filters, gets filtrate, concentrated, obtains concentrated solution;
(2) concentrated solution that step (1) prepares is got, upper H-60D macroporous adsorptive resins, first wash with water, then use 10% successively, 20%, 30%, the ethanol elution of 40%, 50% and 60%, collects the ethanol elution of 40% and 50%, merge, concentrate to obtain macroporous resin eluate;
(3) get the macroporous resin eluate that step (2) obtains, upper polyamide resin column, uses 10% successively, and 20%, 30%, 40%, 50%, 60%, the ethanol elution of 70% and 80%, collects 50% and 60% ethanol elution, concentrates to obtain polyamide eluate;
(4) get the ethanol elution of step (3) 70% and 80%, concentrated, concentrated solution adds hydrochloric acid, adjust pH is 3, then goes up strong acid cation exchange resin column, uses water and ammonia spirit eluting successively, obtain ammonia eluent, concentrated, obtain cation exchange resin eluate;
(5) get step (3) polyamide eluate and step (4) cation exchange resin eluate, add appropriate Icing Sugar and stabilizing agent, cross 40 mesh sieves, granulate, obtains granule, subpackage every bag 10g and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510996480.6A CN105434518A (en) | 2015-12-28 | 2015-12-28 | Traditional Chinese medicine preparation for treating hepatitis |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101549009A (en) * | 2009-04-21 | 2009-10-07 | 常熟雷允上制药有限公司 | Chinese traditional compound medicine preparation for treating hepatitis C and preparation method thereof |
| CN101890063A (en) * | 2009-05-20 | 2010-11-24 | 周亚伟 | Chinese medicament for reducing blood sugar and preparation method thereof |
| CN103083392A (en) * | 2012-12-27 | 2013-05-08 | 浙江工业大学 | Method for separating and enriching part with prenylated flavonoid in subprostrate sophora |
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Patent Citations (3)
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|---|---|---|---|---|
| CN101549009A (en) * | 2009-04-21 | 2009-10-07 | 常熟雷允上制药有限公司 | Chinese traditional compound medicine preparation for treating hepatitis C and preparation method thereof |
| CN101890063A (en) * | 2009-05-20 | 2010-11-24 | 周亚伟 | Chinese medicament for reducing blood sugar and preparation method thereof |
| CN103083392A (en) * | 2012-12-27 | 2013-05-08 | 浙江工业大学 | Method for separating and enriching part with prenylated flavonoid in subprostrate sophora |
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| SOO-MAN KIM，等。: ""Neuroprotective Effects of Methanol Extract of Sophorae Subprostratae Radix on Glutamate Excitotoxicity in PC12 Cells and Organotypic Hippocampal Slice Cultures"", 《THE JOURNAL OF KOREAN ORIENTAL MEDICINE》 * |
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Application publication date: 20160330 |