CN105433380B - A kind of preparation method of three embryo nutritions powder - Google Patents
A kind of preparation method of three embryo nutritions powder Download PDFInfo
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- CN105433380B CN105433380B CN201510916559.3A CN201510916559A CN105433380B CN 105433380 B CN105433380 B CN 105433380B CN 201510916559 A CN201510916559 A CN 201510916559A CN 105433380 B CN105433380 B CN 105433380B
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention discloses a kind of preparation method of three embryo nutritions powder, belongs to technical field of functional food, includes the following steps：(1) chick embryo extract powder is prepared：The polypeptide in chicken embryo is extracted by biological enzymolysis, chick embryo extract powder then is made by cryogenic freezing, drying in polypeptide；(2) grain germ extract powder：Maize is mixed in proportion with wheat, extract powder is made in above-mentioned vegetable protein polypeptide by the vegetable protein polypeptide in double enzyme enzymolysis and extraction mixtures, then through high-pressure fog；The preparation of (3) three embryo nutrition powder：Chick embryo extract powder in step (1), the maize in step (2) are mixed with wheat mixed extract powder with multi-vitamins and minerals, obtain three embryo nutrition powder.The present invention is extracted using chicken embryo, maize, wheat by biological enzymolysis, effectively in animals and plants antioxidant content and trace element combine, be made rich in the ingredients such as glutathione and VE, there is strong anti-oxidation effect.
Description
Technical field
The present invention relates to a kind of preparation method of nutrient powder, more particularly to a kind of preparation method of three embryo nutritions powder belongs to
Technical field of functional food.
Background technology
With advancing age, a series of variation occurs in terms of the morphosis of each organ of body and function, i.e., gradually
Aging.The reduction of aging exhibit tissue cell number, moisture reduce, and organ mass mitigates, hypofunction, but adipose tissue number
Increase trend is but presented in mesh.Modern Free Radical Biology thinks product of the aerobe by oxygen free radical injury with free radical medicine
It is tired that aging death itself, the accumulation of mitochondrial DNA active oxygen radical oxidative damage is caused to cause bio-energy to lack, cell
Aging and death.Free radical destroys the DNA of cell, will lead to the generation of cancer；Free radical destroys protein, will make
Internal enzyme destroys, so as to cause inflammation and astogeny；Free radical destroys fat, and lipid peroxidation will occur, and causes to move
Arteries and veins sample hardens, and apoplexy or heart disease finally occurs；Free radical destroys carbohydrate, and internal hyalomitome will be made to degrade,
Arthritis occurs.Therefore, free radical is most important for body.
All aerobic physiology courses have the generation and removing of free radical, this generation and removing to should be at balancing
State will damage body if the two disequilibrium, and disease occurs, this several years free radical diseases largely occur, the factor of mutagenesis
Including：
1) environmental factor, strong sunlight, the pollution of environment lack necessary battalion in the use of insecticide and soil
It supports.
2) dietary factors lack necessary nutrient in diet, lack moisture, lack protein, and fat is too many.
3) living-pattern preservation, lacks necessary activity, high-pressure of living, excessive drinking, smoke too much and old age from
Group's element occupies and lonely.
By removing free radical energy disease preventing and treating excessive in vivo, but there is presently no good food to help body
It realizes.
Invention content
In view of the above existing problems in the prior art, the present invention provides a kind of preparation method of three embryo nutritions powder, preparation
Three embryo nutrition powder can effectively remove free radical excessive in vivo, achieve the effect that disease preventing and treating.
To achieve the goals above, a kind of preparation method for three embryo nutritions powder that the present invention uses, this method includes following
Step：
(1) chick embryo extract powder is prepared：The polypeptide in chicken embryo is extracted by biological enzymolysis, then leads to polypeptide
Cross cryogenic freezing, chick embryo extract powder is made in drying；
(2) grain germ extract powder：Maize is mixed in proportion with wheat, passes through double enzyme enzymolysis and extraction mixtures
In vegetable protein polypeptide, then extract powder is made in above-mentioned vegetable protein polypeptide through high-pressure fog；
The preparation of (3) three embryo nutrition powder：By the chick embryo extract powder in step (1), the maize in step (2) with it is small
Wheat germ mixed extract powder is mixed with multi-vitamins and minerals, obtains three embryo nutrition powder.
As an improvement, the specific steps that the step (1) prepares chick embryo extract powder include：
(a) 10-12 days chicken embryos are taken, alimentary canal is removed, are cleaned spare after adding 3 times of broken freezings of pigment；
(b) it takes the chicken embryo crushed to be placed in enzymatic vessel, after NaOH solution tune pH value 8-9, chicken embryo weight is added
The pancreatin of 0.02%-0.1%, temperature are to be digested 6 hours at 48-50 DEG C；
(c) after digesting, by chicken embryo in enzymatic vessel under the conditions of temperature is 90-100 DEG C enzyme deactivation 5-15min；
(d) the chicken embryo liquid after enzyme deactivation is cooled to -2 DEG C to fully charge from room temperature, continues to be refrigerated to -18 DEG C, by frozen chicken
Embryo is placed in vacuum drying chamber, and vacuum drying chamber sets pressure and is less than 30Pa, and temperature is 30 DEG C, is made after chicken embryo is dried 20 hours
At chicken embryo powder, as chick embryo extract powder.
As an improvement, in the step (c), chicken embryo enzyme deactivation 10min under the conditions of temperature is 95 DEG C in enzymatic vessel.
As an improvement, the specific steps of step (2) the grain germ extract powder include：
(a) maize and wheat mixed is taken, is soaked 1 hour in the ratio of mixture weight 2.5-10；
(b) impregnate fully after, be added mixing after the protease of maize and the total weight 0.1%-1% of wheat and
The aspergillus enzyme of 0.1%-1% digests 2.5-3.5 hours at 55-65 DEG C；
(c) after the completion of digesting, the enzymolysis solution of maize and wheat is placed at 85-100 DEG C, enzyme deactivation 5-15min；
(d) maize after enzyme deactivation and wheat mixed liquor are spray-dried, and composite cereal germ flour is made.
As an improvement, the specific steps of the preparation of (3) three embryo nutrition powder of the step include：
The chick embryo extract powder handled well, grain germ powder are taken, VE, VC, VB1, VB2, zinc gluconate, breast is added
Sour ferrous, Se-enriched yeast mixing, mixed material is sufficiently mixed to obtain after weighing through three-dimensional mixer.
As an improvement, the mixed material, which crosses 40 mesh, sieves to obtain three embryo nutrition powder.
As an improvement, in the preparation of (3) three embryo nutrition powder of the step, the chick embryo extract handled well in step (1) is taken
Grain germ powder accounting 57.13%, multi-vitamins and minerals accounting 0.02% in powder accounting 42.85%, step (2)
Mixing.
As an improvement, in step (2) the grain germ extract powder, maize is mixed with wheat by 1: 1.
The principle of the present invention is：Wheat embryo and maize germ contain abundant VE, and vitamin E can prevent low density lipoprotein
The formation of albumen, and the wax-like sample substance for being deposited on vascular wall can also be made to reduce, so as to avoid Atheromatosis
The generation and exacerbation of change；Vitamin E can be anti-oxidant, anti-aging, due to the hydrophobic structure of vitamin E, it is possible to be inserted into not
In biomembrane existing for saturated fatty acid, such as cell membrane, sarcolemma, mitochondrial membrane works in adrenal gland and blood lipoprotein,
Generated free radical in lipid peroxidation chain reaction can be removed, and to free radical lipid mistake with anti peroxidation of lipid
Oxidation efficiency is very high.Wheat embryo and maize germ also contain abundant zinc, copper, manganese etc. simultaneously, they are also most important
Polyphenoils mutase important component.
Advantageous effect is：The present invention is using chicken embryo, maize, wheat, by biological enzymolysis extractive technique, effective handle
Antioxidant content and trace element in animals and plants combine, and are made a rich in the ingredients such as glutathione and VE, have strong
A health food of antioxidant effect.
The three embryo nutrition powder of the present invention remove free radical and the characteristics of antioxidation is：1, all systems of body can be helped
It runs well.2, can be by blood-brain barrier, snap action is in corporal parts.3, bioavailability is higher.4, this substance
To be water-soluble, no any secondary use is eaten very safe.
Specific implementation mode
In order to make the objectives, technical solutions and advantages of the present invention clearer, below by embodiment, to the present invention into
Row is further described.However, it should be understood that the specific embodiments described herein are merely illustrative of the present invention, and do not have to
In limiting the scope of the invention.
Embodiment one
1. the preparation of chicken embryo
(1) 10-12 days chicken embryo 100KG are taken, alimentary canal is removed, are cleaned spare after adding the broken freezing of 300KG pigment；
(2) it takes the chicken embryo crushed to be placed in enzymatic vessel, after NaOH solution tune pH value 8-9, chicken embryo weight is added
0.02%-0.1% pancreatin, temperature carry out enzymolysis 6 hours under the conditions of being 48-50 DEG C；
(3) it is 95 DEG C in temperature, enzyme deactivation is carried out under the conditions of time 10min；
(4) the chicken embryo liquid after sterilizing, is being cooled to -2 DEG C very to fully charge from room temperature, is then proceeding to be refrigerated to -18 DEG C,
Vacuum degree pressure is less than 30Pa in vacuum drying chamber, and temperature of heating plate is 30 DEG C, 20 hours drying times, chicken embryo powder is made i.e.
For chick embryo extract powder, chicken embryo powder 45KG is obtained, moisture content is less than 2%.
2. prepared by maize and wheat embryo extract powder：
(1) the maize 50KG mixed and wheat 50KG is taken, water 300KG is added to impregnate 1 hour；
(2) after impregnating fully, the protease and 0.1%- of maize and wheat total weight 0.1%-1% after mixing is added
1% aspergillus enzyme digests 3 hours under the conditions of 60 DEG C；
(3) it is 90 DEG C in temperature, enzyme deactivation is carried out under the conditions of time 10min；
(4) product after enzyme deactivation is spray-dried, and composite cereal germ flour 60KG is made.
3. finished product packing
Learn from else's experience the chicken embryo powder 45Kg handled well, composite cereal embryo powder 60KG, combination vitamin 0.02Kg, after weighing
It is sufficiently mixed in three-dimensional mixer.Uniformly mixed material, is sieved by stainless steel vibratory sieve, it is desirable that all crosses 40 mesh sieve
Net.Material after sieve is dispensed by automatic packaging machine with aluminium foil bag, single bag of net weight 10g.
It is tested for the property by following detection method to three embryo nutrition powder are made：
Glutathione is detected by high performance liquid chromatography：Glutathione is detected using ultraviolet/visible detection device
Content；
Protein detection method：Kjeldahl's method；
VE detection methods：High performance liquid chromatography is detected.
After testing, product Glutathione peptide content：500mg/100g, VE content：100mg/100g, protein content：
80g/100g。
Embodiment two
1. the preparation of chicken embryo
(1) 10-12 days chicken embryo 100KG are taken, alimentary canal is removed, are cleaned spare after adding the broken freezing of 300KG pigment；
(2) it takes the chicken embryo crushed to be placed in enzymatic vessel, after adjusting pH value 8-9, chicken embryo weight 0.02%-0.1% pancreases is added
Enzyme, temperature carry out enzymolysis 6 hours under the conditions of being 48-50 DEG C；
(3) it is 90 DEG C in temperature, enzyme deactivation is carried out under the conditions of time 15min；
(4) the chicken embryo liquid after sterilizing, is being cooled to -2 DEG C very to fully charge from room temperature, is then proceeding to be refrigerated to -18 DEG C,
Vacuum degree pressure is less than 30Pa in vacuum drying chamber, and temperature of heating plate is 30 DEG C, 20 hours drying times, chicken embryo powder is made i.e.
For chick embryo extract powder, chicken embryo powder 45KG is obtained, moisture content is less than 2%.
2. prepared by maize and wheat embryo extract powder：
(1) the maize 50KG mixed and wheat 50KG is taken, water 300KG is added to impregnate 1 hour；
(2) after impregnating fully, the protease and 0.1%- of maize and wheat total weight 0.1%-1% after mixing is added
1% aspergillus enzyme digests 3.5 hours under the conditions of 55 DEG C；
(3) it is 85 DEG C in temperature, enzyme deactivation is carried out under the conditions of time 15min；
(4) product after enzyme deactivation is spray-dried, and composite cereal germ flour 60KG is made.
3. finished product packing
Learn from else's experience the chicken embryo powder 45Kg handled well, composite cereal embryo powder 60KG, combination vitamin 0.02Kg, after weighing
It is sufficiently mixed in three-dimensional mixer.Uniformly mixed material, is sieved by stainless steel vibratory sieve, it is desirable that all crosses 40 mesh sieve
Net.Material after sieve is dispensed by automatic packaging machine with aluminium foil bag, single bag of net weight 10g.
Embodiment three
1. the preparation of chicken embryo
(1) 10-12 days chicken embryo 100KG are taken, alimentary canal is removed, are cleaned spare after adding the broken freezing of 300KG pigment；
(2) it takes the chicken embryo crushed to be placed in enzymatic vessel, after adjusting pH value 8-9, chicken embryo weight 0.02%-0.1% pancreases is added
Enzyme, temperature carry out enzymolysis 6 hours under the conditions of being 48-50 DEG C；
(3) it is 100 DEG C in temperature, enzyme deactivation is carried out under the conditions of time 5min；
(4) the chicken embryo liquid after sterilizing, is being cooled to -2 DEG C very to fully charge from room temperature, is then proceeding to be refrigerated to -18 DEG C,
Vacuum degree pressure is less than 30Pa in vacuum drying chamber, and temperature of heating plate is 30 DEG C, 20 hours drying times, chicken embryo powder is made i.e.
For chick embryo extract powder, chicken embryo powder 45KG is obtained, moisture content is less than 2%.
2. prepared by maize and wheat embryo extract powder：
(1) the maize 50KG mixed and wheat 50KG is taken, water 300KG is added to impregnate 1 hour；
(2) after impregnating fully, the protease and 0.1%- of maize and wheat total weight 0.1%-1% after mixing is added
1% aspergillus enzyme digests 2.5 hours under the conditions of 65 DEG C；
(3) it is 100 DEG C in temperature, enzyme deactivation is carried out under the conditions of time 5min；
(4) product after enzyme deactivation is spray-dried, and composite cereal germ flour 60KG is made.
3. finished product packing
Learn from else's experience the chicken embryo powder 45Kg handled well, composite cereal embryo powder 60KG, combination vitamin 0.02Kg, after weighing
It is sufficiently mixed in three-dimensional mixer.Uniformly mixed material, is sieved by stainless steel vibratory sieve, it is desirable that all crosses 40 mesh sieve
Net.Material after sieve is dispensed by automatic packaging machine with aluminium foil bag, single bag of net weight 10g.
Example IV
The method of the present invention prepares the experiment of three embryo nutrition powder product anti-oxidation functions：
1. material：12 monthly age SPF mouse 80, half male and half female, weight 50-54g, by Nanjing Medical University experimental animal
The heart provides, and SOD and GSH-Px, MDA assay kit are built up Bioengineering Research Institute by Nanjing and provided.
2. setting experiment group：80 mouse are divided into 4 groups, respectively blank control group, experiment I group (tri- embryo nutritions of 3g
Powder/KG weight feeding volume), experiment II groups (tri- embryo nutrition powder of 6g/KG weight feeding volume), experiment III groups (tri- embryo nutrition powder of 10g/
KG weight feeding volume), random to be grouped, every group of 20 mouse take tail blood to survey MDA contents, make Random trimming further according to MDA contents,
Keep the MDA levels of each group almost the same, carry out gavage daily, continuous 60 days, blank control group such as poured at the pure water of weight.
| Group | Number of animals (only) | Dosage (g/kg/ days) |
| Blank control group | 20 | 0 |
| Test I groups | 20 | 3 |
| Test II groups | 20 | 6 |
| Test III groups | 20 | 10 |
3. it is 22 DEG C or so that room temperature, which is arranged, continuous 60 days, every time after feed, illustrate to measure in mouse blood by detection agent box
MDA, SOD activity and GSH-Px activity.
4. experimental result, experimental data carry out variance analysis using statistics software.
Table 1 tests the horizontal measurement results of MDA (n=20, x ± s) in front and back blood
Note：The * * P ＜ 0.05 compared with before experiment of * P ＜ 0.05 compared with blank control group
Table 2 tests SOD activity, GSH-Px determinations of activity result (n=20, x ± s) in front and back blood
| Group (g/kg/ days) | SOD(u/ml) | GSH-Px enzyme activity units |
| Blank control group | 170.35±32.65 | 300.45±26.53 |
| Test I groups | 175.68±25.86* | 340.14±22.67* |
| Test II groups | 185.88±26.32** | 345.67±34.83** |
| Test III groups | 203.45±26.36** | 350.35±60.24** |
Note：The * * P ＜ 0.01 compared with before experiment of * P ＜ 0.05 compared with blank control group
MDA contents compared with blank control group and are substantially reduced (* P ＜ 0.05) in 4.1 experiment I, II, III group mouse blood, in blood
SOD activity, GSH-Px activity also significantly improve (* * P ＜ 0.01).
4.2 can show that three embryo nutrition powder can effectively remove Peroxidation Product MDA according to contractile studies, be both to carry
High SOD activity and GSH-Px activity, judge, three embryo nutrition powder have according to the content in Ministry of Public Health's " function of health food evaluation "
Antioxidation.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modification, equivalent replacement or improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (5)
1. a kind of preparation method of three embryo nutritions powder, which is characterized in that this approach includes the following steps：
(1) chick embryo extract powder is prepared：The polypeptide in chicken embryo is extracted by biological enzymolysis, is then passed through polypeptide low
Chick embryo extract powder is made in temperature freezing, drying；
(2) grain germ extract powder：Maize is mixed in proportion with wheat, by double enzyme enzymolysis and extraction mixtures
Vegetable protein polypeptide, then extract powder is made in above-mentioned vegetable protein polypeptide through high-pressure fog；
The preparation of (3) three embryo nutrition powder：By the chick embryo extract powder in step (1), the maize and wheat in step (2)
Mixed extract powder is mixed with multi-vitamins and minerals, obtains three embryo nutrition powder；
The specific steps that the step (1) prepares chick embryo extract powder include：
(a) 10-12 days chicken embryos are taken, alimentary canal is removed, are cleaned spare after adding 3 times of broken freezings of pigment；
(b) it takes the chicken embryo crushed to be placed in enzymatic vessel, after NaOH solution tune pH value 8-9, chicken embryo weight 0.02%- is added
0.1% pancreatin, temperature are to be digested 6 hours at 48-50 DEG C；
(c) after digesting, by chicken embryo in enzymatic vessel under the conditions of temperature is 90-100 DEG C enzyme deactivation 5-15min；
(d) the chicken embryo liquid after enzyme deactivation is cooled to -2 DEG C to fully charge from room temperature, continues to be refrigerated to -18 DEG C, and freezing chicken embryo is set
In in vacuum drying chamber, vacuum drying chamber sets pressure and is less than 30Pa, and temperature is 30 DEG C, and chicken is made after chicken embryo is dried 20 hours
Embryo powder, as chick embryo extract powder；
The specific steps of step (2) the grain germ extract powder include：
(a) maize and wheat mixed is taken, is soaked 1 hour in 2.5-10 times of ratio of mixture weight；
(b) after impregnating fully, the protease and 0.1%- of maize and the total weight 0.1%-1% of wheat after mixing is added
1% aspergillus enzyme digests 2.5-3.5 hours at 55-65 DEG C；
(c) after the completion of digesting, the enzymolysis solution of maize and wheat is placed at 85-100 DEG C, enzyme deactivation 5-15min；
(d) maize after enzyme deactivation and wheat mixed liquor are spray-dried, and composite cereal germ flour is made.
2. a kind of preparation method of three embryo nutritions powder according to claim 1, which is characterized in that (3) three embryo of the step
The specific steps of the preparation of nutrient powder include：
The chick embryo extract powder handled well, grain germ powder are taken, it is sub- that VE, VC, VB1, VB2, zinc gluconate, lactic acid is added
Iron, Se-enriched yeast mixing, mixed material is sufficiently mixed to obtain after weighing through three-dimensional mixer.
3. a kind of preparation method of three embryo nutritions powder according to claim 2, which is characterized in that the mixed material crosses 40
Mesh sieves to obtain three embryo nutrition powder.
4. a kind of preparation method of three embryo nutritions powder according to claim 1, which is characterized in that (3) three embryo of the step
In the preparation of nutrient powder, grain germ in the chick embryo extract powder accounting 42.85% handled well in step (1), step (2) is taken
Powder accounting 57.13%, multi-vitamins and minerals accounting 0.02% mix.
5. a kind of preparation method of three embryo nutritions powder according to claim 1, which is characterized in that step (2) cereal
In germ extracts powder, maize presses 1 with wheat:1 mixing.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1087006A (en) * | 1993-05-07 | 1994-05-25 | 禄玉 | The preparation method of nurishing liquid of plumule extract |
| CN1792281A (en) * | 2005-12-30 | 2006-06-28 | 山东师范大学 | Beverage contg. plant embryo, and its prepn. method |
| CN102871146A (en) * | 2012-10-22 | 2013-01-16 | 江南大学 | Production process of digestible chick-embryo egg health product |
| CN102894291A (en) * | 2012-10-15 | 2013-01-30 | 江苏麦凯乐生物科技有限公司 | Manufacturing process of three germ peptide |
| CN104585779A (en) * | 2015-01-09 | 2015-05-06 | 孙欣 | Production process for producing germ nutrition powder by virtue of enzymolysis |
-
2015
- 2015-12-11 CN CN201510916559.3A patent/CN105433380B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1087006A (en) * | 1993-05-07 | 1994-05-25 | 禄玉 | The preparation method of nurishing liquid of plumule extract |
| CN1792281A (en) * | 2005-12-30 | 2006-06-28 | 山东师范大学 | Beverage contg. plant embryo, and its prepn. method |
| CN102894291A (en) * | 2012-10-15 | 2013-01-30 | 江苏麦凯乐生物科技有限公司 | Manufacturing process of three germ peptide |
| CN102871146A (en) * | 2012-10-22 | 2013-01-16 | 江南大学 | Production process of digestible chick-embryo egg health product |
| CN104585779A (en) * | 2015-01-09 | 2015-05-06 | 孙欣 | Production process for producing germ nutrition powder by virtue of enzymolysis |
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