CN105433380A - Preparation method for chick embryo-corn germ-wheat germ nutrition powder - Google Patents
Preparation method for chick embryo-corn germ-wheat germ nutrition powder Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method for a chick embryo-corn germ-wheat germ nutrition powder, which belongs to the technical field of functional food. The preparation method comprises the following steps: (1) preparation of chick embryo extract powder: extracting protein polypeptide in chicken embryo through biological enzymatic hydrolysis and subjecting the protein polypeptide to low temperature refrigeration and drying so as to prepare the chick embryo extract powder; (2) preparation of cereal germ extract powder: mixing corn germ and wheat germ in proportion, extracting vegetable protein polypeptide in the mixture through double-enzyme enzymolysis and carrying out high-pressure spraying to prepare the extract powder from the vegetable protein polypeptide; and (3) preparation of the chick embryo-corn germ-wheat germ nutrition powder: a step of mixing the chick embryo extract powder obtained in the step (1), the extract powder of the mixture of the corn germ and wheat germ obtained in the step (2), composite vitamin and mineral matters so as to obtain the chick embryo-corn germ-wheat germ nutrition powder. According to the invention, the chick embryo, the corn germ and the wheat germ are extracted through biological enzymolysis, and antioxidation components and trace elements in animals and plants are effectively combined together; so the prepared hick embryo-corn germ-wheat germ nutrition powder is rich in glutathione, VE and the like and has strong antioxidation effect.
Description
Technical field
The present invention relates to a kind of preparation method of nutrient powder, particularly a kind of preparation method of three embryo nutrition powder, belongs to technical field of functional food.
Background technology
With advancing age, there is a series of change in morphosis and the function aspects of each organ of body, namely old and feeble gradually.The minimizing of old and feeble exhibit tissue cell number, moisture reduces, and organ mass alleviates, hypofunction, but adipose tissue number presents increase trend.Modern Free Radical Biology and free radical medical science think that the accumulation that aerobe is subject to oxygen free radical injury causes aging death own, the accumulation of mitochondrial DNA active oxygen radical oxidative damage, cause bio-energy shortage, cell ageing and death.Free radical destroys the DNA of cell, just can cause the generation of cancer; Free radical destroys protein, the enzyme in body just can be made to destroy, thus cause inflammation and astogeny; Free radical destroys fat, just lipid peroxidation can occur, cause atherosclerosis, finally apoplexy or heart disease occurs; Free radical destroys carbohydrate, and the hyalomitome in body just can be made to degrade, and arthritis occurs.Therefore, radical pair is most important in body.
All aerobic physiology courses all have generation and the removing of free radical, and this generation and removing should be in poised state, if both disequilibriums, just can damage body, and disease occurs, and this several years free radical diseases occur in a large number, and the factor of mutagenesis comprises:
1) environmental factor, strong sunlight irradiates, the pollution of environment, lacks necessary nutrition in utilization of pesticides and soil.
2) dietary factors, lacks necessary nutrient in meals, lack moisture, lacks protein, and fat is too many.
3) living-pattern preservation, lacks necessary activity, life high-pressure, excessive drinking, take drugs, smoke too much and the element that peels off in old age occupy and lonely.
By free radical too much in purged body just energy disease preventing and treating, but good food is not also had to help body realization at present.
Summary of the invention
For above-mentioned prior art Problems existing, the invention provides a kind of preparation method of three embryo nutrition powder, the free radical that three embryo nutrition powder of preparation can be effectively too much in purged body, reaches the effect of disease preventing and treating.
To achieve these goals, the preparation method of a kind of three embryo nutrition powder that the present invention adopts, the method comprises the following steps:
(1) chick embryo extract powder is prepared: extract the polypeptide in chicken embryo by biological enzymolysis, then polypeptide is made chick embryo extract powder by cryogenic freezing, drying;
(2) grain germ extract powder: mixed in proportion with wheat by maize, by the vegetable protein polypeptide in two enzyme enzymolysis and extraction mixture, then makes extract powder through high-pressure fog by above-mentioned vegetable protein polypeptide;
The preparation of (3) three embryo nutrition powder: the maize in the chick embryo extract powder in step (1), step (2) is mixed with B B-complex and mineral matter with wheat mixed extract powder, obtains three embryo nutrition powder.
As improvement, the concrete steps that described step (1) prepares chick embryo extract powder comprise:
A () gets the chicken embryo of 10-12 days, remove alimentary canal, cleans that to add 3 times of pigment broken freezing rear for subsequent use;
B () is got the chicken embryo crushed and is placed in enzymatic vessel, after NaOH solution adjust pH 8-9, add the pancreatin of chicken embryo weight 0.02%-0.1%, and temperature is enzymolysis 6 hours at 48-50 DEG C;
C () enzymolysis terminates after, go out chicken embryo in enzymatic vessel under temperature is 90-100 DEG C of condition enzyme 5-15min;
D () is gone out the chicken blastochyle after enzyme,-2 DEG C are cooled to fully charge from room temperature, continue to be refrigerated to-18 DEG C, frozen chicken embryo is placed in vacuum drying chamber, vacuum drying chamber setting pressure is less than 30Pa, temperature is 30 DEG C, chicken embryo is made chicken embryo powder after dry 20 hours, is chick embryo extract powder.
As improvement, in described step (c), in enzymatic vessel, chicken embryo goes out enzyme 10min under temperature is 95 DEG C of conditions.
As improvement, the concrete steps of described step (2) grain germ extract powder comprise:
A () gets the maize and wheat that mix, soak 1 hour in the ratio of mixture weight 2.5-10;
B () soaks fully, add the total protease of weight 0.1%-1% of maize and wheat after mixing and aspergillus enzyme enzymolysis 2.5-3.5 hour at 55-65 DEG C of 0.1%-1%;
C () enzymolysis completes after, at the enzymolysis solution of maize and wheat is placed in 85-100 DEG C, go out enzyme 5-15min;
D () the go out maize after enzyme and wheat mixed liquor carries out spraying dry, obtained composite cereal germ flour.
As improvement, the concrete steps of the preparation of described step (3) three embryo nutrition powder comprise:
Get chick embryo extract powder, the grain germ powder handled well, add VE, VC, VB1, VB2, zinc gluconate, ferrous lactate, Se-enriched yeast mixing, after weighing, be fully mixed to get mixed material through three-dimensional mixer.
As improvement, described mixed material is crossed 40 mesh sieves and is obtained three embryo nutrition powder.
As improvement, in the preparation of described step (3) three embryo nutrition powder, get chick embryo extract powder accounting 42.85%, step (2) the middle cereal germ flour end accounting 57.13% handled well in step (1), B B-complex and mineral matter accounting 0.02% mix.
As improvement, in described step (2) grain germ extract powder, maize mixes by 1:1 with wheat.
Principle of the present invention is: wheat embryo and maize germ contain abundant VE, vitamin E can stop the formation of low-density lipoprotein, and the wax-like sample material being deposited on vascular wall can also be made to reduce, thus avoid the generation of atherosclerotic lesion and increase the weight of; Vitamin E can be anti-oxidant, anti-aging, due to the hydrophobic structure of vitamin E, so can be inserted in the biomembrane of unrighted acid existence, as cell membrane, sarcolemma, mitochondrial membrane, works in adrenal gland and blood lipoprotein, can anti peroxidation of lipid, namely the free radical produced in lipid peroxidation chain reaction is removed, and very high to free radical lipid peroxidation efficiency.Wheat embryo and maize germ are also containing abundant zinc, copper, manganese etc. simultaneously, and they are also the important component parts of most important polyphenoils mutase.
Beneficial effect is: the present invention adopts chicken embryo, maize, wheat, by biological enzymolysis extractive technique, effectively the antioxidant content in animals and plants and trace element are combined, make and be aly rich in the composition such as glutathione and VE, there is a health food of strong anti-oxidation effect.
The feature of three embryo nutrition powder scavenging free radicals of the present invention and antioxidation is: all systems of health 1, can be helped to run well.2, by blood-brain barrier, snap action is in corporal parts.3, bioavailability is higher.4, this material is water miscible, without any secondary use, edible very safe.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly understand, below by embodiment, the present invention is further elaborated.But should be appreciated that, specific embodiment described herein, only in order to explain the present invention, is not limited to scope of the present invention.
Embodiment one
1. the preparation of chicken embryo
(1) get the chicken embryo 100KG of 10-12 days, remove alimentary canal, clean that to add 300KG pigment broken freezing rear for subsequent use;
(2) get the chicken embryo crushed and be placed in enzymatic vessel, after adjusting pH value 8-9 by NaOH solution, add chicken embryo weight 0.02%-0.1% pancreatin, temperature is carry out enzymolysis 6 hours under 48-50 DEG C of condition;
(3) be 95 DEG C in temperature, under time 10min condition, carry out going out enzyme;
(4) the chicken blastochyle after sterilizing,-2 DEG C are being cooled to very to fully charge from room temperature, then continue to be refrigerated to-18 DEG C, in vacuum drying chamber, vacuum pressure is less than 30Pa, temperature of heating plate is 30 DEG C, 20 hours drying times, makes chicken embryo powder and is chick embryo extract powder, obtain chicken embryo powder 45KG, moisture content is less than 2%.
2. maize and the preparation of wheat extract powder:
(1) get the maize 50KG mixed and wheat 50KG, the 300KG that adds water soaks 1 hour;
(2) soak fully, to add after mixing maize and the protease of wheat gross weight 0.1%-1% and the aspergillus enzyme of 0.1%-1% enzymolysis 3 hours under 60 DEG C of conditions;
(3) be 90 DEG C in temperature, under time 10min condition, carry out going out enzyme;
(4) product after enzyme that goes out carries out spraying dry, makes composite cereal germ flour 60KG.
3. finished product packing
Learn from else's experience handle well chicken embryo powder 45Kg, composite cereal embryo powder 60KG, combination vitamin 0.02Kg, fully mix in three-dimensional mixer after weighing.The material mixed, is sieved by stainless steel vibratory sieve, requires whole mistake 40 eye mesh screen.Material after sieve, carries out packing by automatic packaging machine aluminium foil bag, single bag of net weight 10g.
By following detection method, performance test is carried out to obtained three embryo nutrition powder:
Glutathione is detected by high performance liquid chromatography: adopt ultraviolet/visible detection device to detect the content of glutathione;
Protein detection method: Kjeldahl's method;
VE detection method: high performance liquid chromatography detects.
After testing, product Glutathione peptide content: 500mg/100g, VE content: 100mg/100g, protein content: 80g/100g.
Embodiment two
1. the preparation of chicken embryo
(1) get the chicken embryo 100KG of 10-12 days, remove alimentary canal, clean that to add 300KG pigment broken freezing rear for subsequent use;
(2) get the chicken embryo crushed and be placed in enzymatic vessel, after adjusting pH value 8-9, add chicken embryo weight 0.02%-0.1% pancreatin, temperature is carry out enzymolysis 6 hours under 48-50 DEG C of condition;
(3) be 90 DEG C in temperature, under time 15min condition, carry out going out enzyme;
(4) the chicken blastochyle after sterilizing,-2 DEG C are being cooled to very to fully charge from room temperature, then continue to be refrigerated to-18 DEG C, in vacuum drying chamber, vacuum pressure is less than 30Pa, temperature of heating plate is 30 DEG C, 20 hours drying times, makes chicken embryo powder and is chick embryo extract powder, obtain chicken embryo powder 45KG, moisture content is less than 2%.
2. maize and the preparation of wheat extract powder:
(1) get the maize 50KG mixed and wheat 50KG, the 300KG that adds water soaks 1 hour;
(2) soak fully, to add after mixing maize and the protease of wheat gross weight 0.1%-1% and the aspergillus enzyme of 0.1%-1% enzymolysis 3.5 hours under 55 DEG C of conditions;
(3) be 85 DEG C in temperature, under time 15min condition, carry out going out enzyme;
(4) product after enzyme that goes out carries out spraying dry, makes composite cereal germ flour 60KG.
3. finished product packing
Learn from else's experience handle well chicken embryo powder 45Kg, composite cereal embryo powder 60KG, combination vitamin 0.02Kg, fully mix in three-dimensional mixer after weighing.The material mixed, is sieved by stainless steel vibratory sieve, requires whole mistake 40 eye mesh screen.Material after sieve, carries out packing by automatic packaging machine aluminium foil bag, single bag of net weight 10g.
Embodiment three
1. the preparation of chicken embryo
(1) get the chicken embryo 100KG of 10-12 days, remove alimentary canal, clean that to add 300KG pigment broken freezing rear for subsequent use;
(2) get the chicken embryo crushed and be placed in enzymatic vessel, after adjusting pH value 8-9, add chicken embryo weight 0.02%-0.1% pancreatin, temperature is carry out enzymolysis 6 hours under 48-50 DEG C of condition;
(3) be 100 DEG C in temperature, under time 5min condition, carry out going out enzyme;
(4) the chicken blastochyle after sterilizing,-2 DEG C are being cooled to very to fully charge from room temperature, then continue to be refrigerated to-18 DEG C, in vacuum drying chamber, vacuum pressure is less than 30Pa, temperature of heating plate is 30 DEG C, 20 hours drying times, makes chicken embryo powder and is chick embryo extract powder, obtain chicken embryo powder 45KG, moisture content is less than 2%.
2. maize and the preparation of wheat extract powder:
(1) get the maize 50KG mixed and wheat 50KG, the 300KG that adds water soaks 1 hour;
(2) soak fully, to add after mixing maize and the protease of wheat gross weight 0.1%-1% and the aspergillus enzyme of 0.1%-1% enzymolysis 2.5 hours under 65 DEG C of conditions;
(3) be 100 DEG C in temperature, under time 5min condition, carry out going out enzyme;
(4) product after enzyme that goes out carries out spraying dry, makes composite cereal germ flour 60KG.
3. finished product packing
Learn from else's experience handle well chicken embryo powder 45Kg, composite cereal embryo powder 60KG, combination vitamin 0.02Kg, fully mix in three-dimensional mixer after weighing.The material mixed, is sieved by stainless steel vibratory sieve, requires whole mistake 40 eye mesh screen.Material after sieve, carries out packing by automatic packaging machine aluminium foil bag, single bag of net weight 10g.
Embodiment four
The inventive method prepares three embryo nutrition powder product anti-oxidation function experiments:
1. material: 12 monthly age SPF mouse 80, male and female half and half, body weight 50-54g, is provided by Nanjing Medical University's Experimental Animal Center, SOD and GSH-Px, MDA mensuration kit builds up Bioengineering Research Institute by Nanjing to be provided.
2. experiment group is set: 80 mouse are divided into 4 groups, be respectively blank group, experiment I group (3g tri-embryo nutrition powder/KG body weight feeding volume), experiment II group (6g tri-embryo nutrition powder/KG body weight feeding volume), experiment III group (10g tri-embryo nutrition powder/KG body weight feeding volume), random packet, often organize 20 mouse, get tail blood and survey MDA content, Random trimming is made again according to MDA content, make the MDA level of each group basically identical, every day carries out gavage, continuous 60 days, blank group such as to pour at the pure water of weight.
| Group | Number of animals (only) | Dosage (g/kg/ days) |
| Blank group | 20 | 0 |
| Experiment I group | 20 | 3 |
| Experiment II group | 20 | 6 |
| Experiment III group | 20 | 10 |
3. arranging room temperature is about 22 DEG C, continuous 60 days, after each feed, illustrates that MDA, the SOD measured in Mouse Blood is active and GSH-Px is active by detection agent box.
4. experimental result, experimental data adopt statistics software to carry out variance analysis.
MDA level determination result (n=20, x ± s) in the blood of front and back tested by table 1
Note: compare * P<0.05 with blank group and compare * * P<0.05 with before experiment
Table 2 tests SOD activity, GSH-Px determination of activity result (n=20, x ± s) in the blood of front and back
| Group (g/kg/ days) | SOD(u/ml) | GSH-Px enzyme activity unit |
| Blank group | 170.35±32.65 | 300.45±26.53 |
| Experiment I group | 175.68±25.86 * | 340.14±22.67 * |
| Experiment II group | 185.88±26.32 ** | 345.67±34.83 ** |
| Experiment III group | 203.45±26.36 ** | 350.35±60.24 ** |
Note: compare * P<0.05 with blank group and compare * * P<0.01 with before experiment
MDA content comparatively blank group and obviously reduction (* P<0.05) in 4.1 experiment I, II, III group Mouse Bloods, in blood, SOD is active, GSH-Px is active also significantly improves (* * P<0.01).
According to contractile studies, 4.2 can show that three embryo nutrition powder can effectively remove Peroxidation Product MDA, be both and improve SOD activity and GSH-Px activity, judge according to the content in Ministry of Public Health's " function of health food evaluation ", three embryo nutrition powder have antioxidation.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement or improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. a preparation method for three embryo nutrition powder, it is characterized in that, the method comprises the following steps:
(1) chick embryo extract powder is prepared: extract the polypeptide in chicken embryo by biological enzymolysis, then polypeptide is made chick embryo extract powder by cryogenic freezing, drying;
(2) grain germ extract powder: mixed in proportion with wheat by maize, by the vegetable protein polypeptide in two enzyme enzymolysis and extraction mixture, then makes extract powder through high-pressure fog by above-mentioned vegetable protein polypeptide;
The preparation of (3) three embryo nutrition powder: the maize in the chick embryo extract powder in step (1), step (2) is mixed with B B-complex and mineral matter with wheat mixed extract powder, obtains three embryo nutrition powder.
2. the preparation method of a kind of three embryo nutrition powder according to claim 1, it is characterized in that, the concrete steps that described step (1) prepares chick embryo extract powder comprise:
A () gets the chicken embryo of 10-12 days, remove alimentary canal, cleans that to add 3 times of pigment broken freezing rear for subsequent use;
B () is got the chicken embryo crushed and is placed in enzymatic vessel, after NaOH solution adjust pH 8-9, add the pancreatin of chicken embryo weight 0.02%-0.1%, and temperature is enzymolysis 6 hours at 48-50 DEG C;
C () enzymolysis terminates after, go out chicken embryo in enzymatic vessel under temperature is 90-100 DEG C of condition enzyme 5-15min;
D () is gone out the chicken blastochyle after enzyme,-2 DEG C are cooled to fully charge from room temperature, continue to be refrigerated to-18 DEG C, frozen chicken embryo is placed in vacuum drying chamber, vacuum drying chamber setting pressure is less than 30Pa, temperature is 30 DEG C, chicken embryo is made chicken embryo powder after dry 20 hours, is chick embryo extract powder.
3. the preparation method of a kind of three embryo nutrition powder according to claim 2, is characterized in that, in described step (c), in enzymatic vessel, chicken embryo goes out enzyme 10min under temperature is 95 DEG C of conditions.
4. the preparation method of a kind of three embryo nutrition powder according to claim 1 and 2, is characterized in that, the concrete steps of described step (2) grain germ extract powder comprise:
A () gets the maize and wheat that mix, soak 1 hour in the ratio of mixture weight 2.5-10;
B () soaks fully, add the total protease of weight 0.1%-1% of maize and wheat after mixing and aspergillus enzyme enzymolysis 2.5-3.5 hour at 55-65 DEG C of 0.1%-1%;
C () enzymolysis completes after, at the enzymolysis solution of maize and wheat is placed in 85-100 DEG C, go out enzyme 5-15min;
D () the go out maize after enzyme and wheat mixed liquor carries out spraying dry, obtained composite cereal germ flour.
5. the preparation method of a kind of three embryo nutrition powder according to claim 4, is characterized in that, the concrete steps of the preparation of described step (3) three embryo nutrition powder comprise:
Get chick embryo extract powder, the grain germ powder handled well, add VE, VC, VB1, VB2, zinc gluconate, ferrous lactate, Se-enriched yeast mixing, after weighing, be fully mixed to get mixed material through three-dimensional mixer.
6. the preparation method of a kind of three embryo nutrition powder according to claim 5, it is characterized in that, described mixed material is crossed 40 mesh sieves and is obtained three embryo nutrition powder.
7. the preparation method of a kind of three embryo nutrition powder according to claim 1, it is characterized in that, in the preparation of described step (3) three embryo nutrition powder, get chick embryo extract powder accounting 42.85%, step (2) the middle cereal germ flour end accounting 57.13% handled well in step (1), B B-complex and mineral matter accounting 0.02% mix.
8. the preparation method of a kind of three embryo nutrition powder according to claim 1, is characterized in that, in described step (2) grain germ extract powder, maize mixes by 1:1 with wheat.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1087006A (en) * | 1993-05-07 | 1994-05-25 | 禄玉 | The preparation method of nurishing liquid of plumule extract |
| CN1792281A (en) * | 2005-12-30 | 2006-06-28 | 山东师范大学 | Beverage contg. plant embryo, and its prepn. method |
| CN102871146A (en) * | 2012-10-22 | 2013-01-16 | 江南大学 | Production process of digestible chick-embryo egg health product |
| CN102894291A (en) * | 2012-10-15 | 2013-01-30 | 江苏麦凯乐生物科技有限公司 | Manufacturing process of three germ peptide |
| CN104585779A (en) * | 2015-01-09 | 2015-05-06 | 孙欣 | Production process for producing germ nutrition powder by virtue of enzymolysis |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1087006A (en) * | 1993-05-07 | 1994-05-25 | 禄玉 | The preparation method of nurishing liquid of plumule extract |
| CN1792281A (en) * | 2005-12-30 | 2006-06-28 | 山东师范大学 | Beverage contg. plant embryo, and its prepn. method |
| CN102894291A (en) * | 2012-10-15 | 2013-01-30 | 江苏麦凯乐生物科技有限公司 | Manufacturing process of three germ peptide |
| CN102871146A (en) * | 2012-10-22 | 2013-01-16 | 江南大学 | Production process of digestible chick-embryo egg health product |
| CN104585779A (en) * | 2015-01-09 | 2015-05-06 | 孙欣 | Production process for producing germ nutrition powder by virtue of enzymolysis |
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