CN105432472A - Psammosilene tunicoides W. C. Wu et C. Y. Wu adventitious root rapid proliferation culturing method - Google Patents
Psammosilene tunicoides W. C. Wu et C. Y. Wu adventitious root rapid proliferation culturing method Download PDFInfo
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Abstract
The invention discloses a psammosilene tunicoides W. C. Wu et C. Y. Wu adventitious root rapid proliferation culturing method, and belongs to the field of plant organ and tissue proliferation culturing method. According to the invention, MS+1.0mg/L IBA+30g/L white granulated sugar or MS+1.0mg/L KT+30g/L white granulated sugar solid culture medium is inoculated with psammosilene tunicoides W. C. Wu et C. Y. Wu adventitious root, the adventitious root dry weight and dry matter rate of the MS+1.0mg/L KT+30g/L white granulated sugar solid culture medium are relatively high; and the dry matter rate of culturing taking 30g/L white granulated sugar as the MS solid culture medium carbon source is higher than that of culturing taking glucose as the carbon source, and cost of culturing taking 30g/L white granulated sugar as the MS solid culture medium carbon source is lower than that of culturing taking sucrose as the carbon source. Abundant psammosilene tunicoides W. C. Wu et C. Y. Wu adventitious root can be obtained in 40 days under the above conditions. The psammosilene tunicoides W. C. Wu et C. Y. Wu adventitious root rapid proliferation culturing method is capable of providing industrialized production practice with basis and guidance.
Description
Technical field
The invention belongs to plant organ tissue cultures, particularly medicinal plant adventive root medium.
Background technology
Tuniclike psammosilene root (PsammosilenetunicoidesW.C.WuetC.Y.Wu) belongs to the perennial medicinal herb plant of (Psammosilene) autogenus for Caryophyllaceae (Caryophyllaceae) tuniclike psammosilene root, has another name called Kunming the root of straight ladybell, RADIX PSAMMOSILENE etc.Tuniclike psammosilene root root containing saponin constituent, and containing amino acid, organic acid, triterpene etc., is used as medicine after its root drying, is the main component of Yunnan Baiyao, short-pedicel aconite root, pain Urapidil, golden bone lotus capsule etc.China lacks natural resources of Chinese medicinal materials effective protection measure, and wild resource is endangered, produces the scarcity that can make up plant resources manually to cultivate with industrialization.The cultivation of tuniclike psammosilene root adventive root is mainly by the impact of the several respects such as explant, inoculum concentration, plant hormone, sucrose and mineral salt.In relevant report, Zhao Shuan and Li Jingbin once utilized agrobacterium rhizogenes tuniclike psammosilene root to obtain hair shape root respectively, and studied the optimal culture conditions of tuniclike psammosilene root hair shape root.But this technology needs comparatively high-tech and appointed condition, still has limitation.(Zhao Shuan etc. the Primary Study [J] of tuniclike psammosilene root hair shape root induction. traditional Chinese medicine, 2012,35(2): 176-179; Li Jingbin etc. the foundation [J] of tuniclike psammosilene root hair shape root induction and cultivating system. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2011,36 (5): 547-551).Document: " affecting the factor of tuniclike psammosilene root growth of hair root and saponin(e accumulation " induces a mao shape root with agrobacterium rhizogenes ACCC10060 strain infection tuniclike psammosilene root stem section, blade, through anti-kanamycin screening and culturing, the hair shape root obtained is adventive root liquid culture material, inoculating 5 kinds respectively with the addition of in the liquid nutrient medium of exogenous hormone, to add the 1/2MS liquid nutrient medium of NAA, IAA, IBA to tuniclike psammosilene root adventive root propagation better, and find that growth rate and saponin(e output are proportionate.(Tian Sidi etc., time precious traditional Chinese medical science traditional Chinese medicines [J], 2012,23 (4): 824-826.).Another document: " different condition of culture is on the impact of tuniclike psammosilene root growth of hair root " (Gao Shuai, Wang Hongfeng, Hou Lili, Deng, Annual [J], 2012,28 (2): 16-21.) select 1/2MS solid culture medium to be optimal medium, the adventive root after infecting with agrobacterium rhizogenes is cultivated for material.
Sugar is that explant growth grows the element forming carbon skeleton, the osmotic pressure of maintain base simultaneously.Different types of sugar, molecular structure is different, its form and concentration comparatively obvious to adventive root growth effect.The carbon source of sucrose as medium thought by above two sections of documents, and the accumulation of tuniclike psammosilene root hair shape root biomass is maximum.
Summary of the invention
The present invention is intended to the method setting up the cultivation of a kind of tuniclike psammosilene root adventive root fast breeding, it comprises by minimal medium and the medium system that forms with variety classes hormone combinations thereof, so that produce according to the corresponding medium of production actual selection, thus reduction production cost, raise the efficiency, for factorial praluction tuniclike psammosilene root adventive root provides guidance.
The present invention realizes with following method:
(1) terminal bud cutting tuniclike psammosilene root aseptic seedling is inoculated in the root media of 1/2MS+0.3mg/LIBA+0.1mg/LNAA+0.3g/L active carbon, forms the indefinite network of roots of hair shape after 60d;
(2) by block mode cut expand numerous after the indefinite network of roots of tuniclike psammosilene root, be inoculated in and with the addition of in the MS solid-based basal culture medium of hormone and white granulated sugar, the medium of every bottle of packing 25mL, every bottle graft kind 4 pieces of indefinite networks of roots of tuniclike psammosilene root.
Further, described medium is following one:
(1) combination of MS solid-based basal culture medium and growth hormone: MS+30g/L white granulated sugar+1.0mg/LIBA;
(2) combination of MS solid-based basal culture medium and the basic element of cell division: MS+30g/L white granulated sugar+1.0mg/LKT;
(1) ~ (2) condition of culture is above: 25 ± 2 DEG C, light application time 12h/d, intensity of illumination 1500 ~ 2000lx, incubation time 40d.
Preferably, described medium is the combination of MS solid-based basal culture medium and the basic element of cell division: MS+1.0mg/LKT.
Further, described medium is: the tuniclike psammosilene root adventive root tissue block being inoculated in described medium is 0.05g/ block, and this tissue block dry weight is roughly equal to 0.0056 ± 0.0004g/ block.
Marked improvement of the present invention is:
(1) the present invention is thought by the medium culture result of 2 kinds of different constituents: except considering the genotype difference of selected materials, should with the minimal medium of MS solid culture medium for tuniclike psammosilene root hair shape root, under this minimal medium, even if do not add its fresh weight of hormone can reach high value yet, this from generally select 1/2MS medium different at present.
(2) get sucrose from prior art and glucose is different, the present invention considers from adventive root growth result and production cost: the white granulated sugar adding 30g/L MS medium is more suitable for growth and the biomass accumulation of tuniclike psammosilene root adventive root.Under the white granulated sugar of 30g/L, do not add the high value that its fresh weight of hormone can reach 5.088g, its dry weight is 0.480g, is also high value.It reflects: white granulated sugar, as the supplementary carbon source of medium, with the consumption of 30g/L, can effectively reduce costs, and can obtain again higher hair shape root output.
(3) the present invention additional 1.0mg/LIBA in the MS minimal medium that with the addition of 30g/L white granulated sugar cultivates tuniclike psammosilene root adventive root, also can obtain good effect.
(4) index evaluating tuniclike psammosilene root adventive root growing state comprises fresh weight, the amount of growth of dry weight and dry rate.Wherein, dry rate refers to the dry weight of adventive root and the ratio of fresh weight, it reflects the net yield of adventive root.On the MS solid culture medium that with the addition of 30g/L white granulated sugar, the present invention changes the plant hormone of tuniclike psammosilene root tissue cultures, in the MS medium adding 1.0mg/LKT adventive root dry weight and dry rate the highest, and process significant difference with other, KT is more conducive to the growth of tuniclike psammosilene root adventive root.Relative to the data of existing document, the dry rate of the tuniclike psammosilene root adventive root that the solid culture medium of this MS+1.0mg/LKT obtains is 11.96%, and its fresh weight is 173.2gL
-1, dry weight is 20.72gL
-1, dry weight is 1.52 times of existing document.
Accompanying drawing explanation
Fig. 1 is adventive root growing state in WPM minimal medium.
Fig. 2 is adventive root growing state in MS minimal medium.
Fig. 3 is adventive root growing state in the medium of MS+30g/L white granulated sugar.
Fig. 4 is adventive root growing state in MS+IBA1.0mg/L medium.
Fig. 5 is adventive root growing state in MS+KT1.0mg/L medium.
Fig. 6 is adventive root growing state in MS+NAA0.5mg/L+KT0.5mg/L medium.
Above MS refers to MS solid culture medium, and KT refers to kinetin, and IBA refers to 3-indolebutyric acid, and NAA refers to α-naphthaleneacetic acid.
Below in conjunction with embodiment, the present invention will be further described.The present invention includes but be not limited to the content of embodiment.
Embodiment
1 materials and methods
1.1 material
Aseptically, the terminal bud cutting tuniclike psammosilene root aseptic seedling is inoculated in culture of rootage in the root media of 1/2MS+0.3mg/LIBA+0.1mg/LNAA+0.3g/L active carbon, after 60 days, is trained the indefinite network of roots of mao shape, for subsequent use.
1.2 method
During inoculation, cut above-mentioned indefinite network of roots by block mode and cultivate.
1.2.1 different minimal medium is on the impact of tuniclike psammosilene root adventive root biomass
Cut tuniclike psammosilene root adventive root tissue block, every block is about 0.05g, dry weight is roughly equal to 0.0056 ± 0.0004g/ block, be inoculated in respectively and with the addition of in MS, 1/2MS, WPM tri-kinds of solid-based basal culture mediums of 30g/L sucrose respectively, often process 5 bottles, every bottle of packing 25mL medium, every bottle graft kind 4 pieces, repeat 3 times, when 40d, measure fresh weight, the dry weight of tuniclike psammosilene root adventive root.Condition of culture is: temperature 25 ± 2 DEG C, light application time 12h/d, intensity of illumination 1500 ~ 2000lx.Lower same.
1.2.2 variable concentrations carbon source kind is on the impact of tuniclike psammosilene root adventive root biomass
Cut tuniclike psammosilene root adventive root tissue block, every block is about 0.05g, and dry weight is roughly equal to 0.0056 ± 0.0004g/ block, is inoculated on the minimal medium of different carbon source respectively.It is 20,30 and the white granulated sugar of 40g/L, sucrose and glucose that this medium adds concentration respectively, and test amounts to 9 kinds of process, often process 5 bottles, every bottle of packing 25mL medium, every bottle graft 4 pieces, repeats 3 times, measures fresh weight, the dry weight of tuniclike psammosilene root adventive root when 40d.
1.2.3 variable concentrations growth hormone is on the impact of tuniclike psammosilene root adventive root biomass
Screen the minimal medium and carbon source that obtain based on 1.2.1 and 1.2.2, IBA and NAA of additional variable concentrations is as the medium that addition of growth hormone.
Cut tuniclike psammosilene root adventive root tissue block, every block is about 0.05g, and its dry weight is roughly equal to 0.0056 ± 0.0004g/ block, is inoculated in respectively and addition of on the medium of growth hormone.Test amounts to 9 kinds of medium treatments.Often process 5 bottles, every bottle of packing 25mL medium, every bottle graft 4 pieces, repeat number is 3, measures fresh weight and the dry weight of tuniclike psammosilene root adventive root when 40d.
1.2.4 the variable concentrations basic element of cell division is on the impact of tuniclike psammosilene root adventive root biomass
Screen the minimal medium and carbon source that obtain based on 1.2.1 and 1.2.2, KT, 6-BA and TDZ of additional variable concentrations are as the medium that addition of the basic element of cell division.
Cut tuniclike psammosilene root adventive root tissue block, every block is about 0.05g, and its dry weight is roughly equal to 0.0056 ± 0.0004g/ block, is inoculated in respectively and addition of on the medium of the basic element of cell division.Test amounts to 11 kinds of medium treatments, and often process 5 bottles, every bottle of packing 25mL medium, every bottle graft 4 pieces, repeat number is 3, measures fresh weight and the dry weight of tuniclike psammosilene root adventive root when 40d.
1.2.5 hormon proportioning is on the impact of tuniclike psammosilene root adventive root biomass
Screen the minimal medium and carbon source that obtain based on 1.2.1 and 1.2.2, KT, 6-BA, IBA, NAA and TDZ of additional variable concentrations are as the medium that addition of exogenous hormone.
Cut tuniclike psammosilene root adventive root tissue block, every block is about 0.05g, its dry weight is roughly equal to 0.0056 ± 0.0004g/ block, be inoculated in respectively and addition of on the medium of exogenous hormone, test designs 8 kinds of process medium altogether, often processes 5 bottles, every bottle of packing 25mL medium, every bottle graft kind 4 pieces, repeats 3 times, measures fresh weight and the dry weight of tuniclike psammosilene root adventive root when 40d.
1.3 data analysis
The data obtained all carries out statistical analysis in SPSS17.0 and EXCELL2007 software, wherein:
Fresh weight: the weight of fresh adventive root in 1 bottle of medium
Dry weight: the weight in 1 bottle of medium after fresh adventive root oven dry
Dry rate=dry weight/fresh weight
Every gram of sugared actuating quantity=1 liter cultivate in adventive root dry weight recruitment/1 liter medium in sugared consumption
2 results and analysis
2.1 different minimal mediums are on the impact of tuniclike psammosilene root adventive root biomass
Tuniclike psammosilene root adventive root is inoculated in MS, 1/2MS, WPM medium respectively, inoculates 4d and all can grow a little tender white adventive root.It the results are shown in Table 1.
The different minimal medium of table 1 is on the impact of tuniclike psammosilene root adventive root biomass
| Kinds of culture medium | Fresh weight (g) | Dry weight (g) | Dry rate % | Root growth situation |
| MS | 4.968a | 0.474a | 9.54a | Branch is many and thick, faint yellow |
| 1/2MS | 4.018a | 0.361b | 8.98b | Branch is many and thick, faint yellow |
| WPM | 3.599b | 0.359c | 9.97a | Branch is many and thin, aubergine |
Note: the different letter representatives of same column significant difference in 0.05 level in table, lower same.
As shown in Table 1, in 3 kinds of medium, adventive root branch is all more, evaluates, think that MS is the more excellent minimal medium (Fig. 1) of tuniclike psammosilene root adventive root growth from the growth of tuniclike psammosilene root adventive root growing state and its fresh weight, dry weight and dry rate.
2.2 variable concentrations carbon source kinds are on the impact of tuniclike psammosilene root adventive root biomass
In MS minimal medium, additional variable concentrations and different types of carbon source, after inoculation, the accumulation of tuniclike psammosilene root adventive root biomass is in table 2.
Table 2 different carbon source kind and concentration are on the impact of tuniclike psammosilene root adventive root biomass
As seen from Table 2, when cultivating 40d, white granulated sugar and sucrose are when concentration is 30g/L and 40g/L, and its fresh weight and dry weight are all higher, and difference is not significantly (being respectively 5.088g and 0.48g, 4.899g and 0.392g, 5.158g and 0.486g and 5.956g and 0.515g); And take glucose as the medium of carbon source, the accumulation effect of tuniclike psammosilene root adventive root is poor.From every gram of sugared action effect, add in MS minimal medium respectively 20g/L, 30g/L sucrose and 30g/L white granulated sugar between the action effect of adventive root biomass accumulation without significant difference, what action effect was the poorest is the MS medium adding 40g/L glucose, and the actuating quantity of its sugar is only 0.250.Due in the consumption situation that carbon source is same, the cost of sucrose will far above white granulated sugar, therefore, and binding tests result, and consider from the economic angle of production cost, 30g/L white granulated sugar is the suitableeest carbon source (Fig. 2) that tuniclike psammosilene root adventive root is cultivated on MS medium.
2.3 variable concentrations growth hormone are on the impact of tuniclike psammosilene root adventive root biomass
In the MS medium that addition of variable concentrations growth hormone respectively, tuniclike psammosilene root adventive root biomass accumulation situation is in table 3.
Table 3 variable concentrations growth hormone is on the impact of tuniclike psammosilene root adventive root biomass
Table 3 shows, after tuniclike psammosilene root adventive root cultivates 40d, when IBA concentration is 1.0mg/L, better, the fresh weight of the lower adventive root of this process is 4.754g, and dry weight is 0.504g in adventive root growth, all be significantly higher than other process, and be about 1.5 times of contrast (2.918g and 0.337g) respectively; But when IBA concentration is 3.0mg/L, tuniclike psammosilene root adventive root biomass dry weight is only 0.306g, it is the minimum in all process.Visible, suitable exogenous auxin and concentration thereof are the requirements of tuniclike psammosilene root adventive root growth.Table 3 also shows, when medium is MS+IBA1.0mg/L, its dry rate also shows as 10.60% higher level.Consider tuniclike psammosilene root adventive root biomass growth pattern, in the MS solid culture medium being attached with 1.0mg/LIBA+30g/L white granulated sugar, the growth of tuniclike psammosilene root adventive root better (Fig. 3).
The 2.4 variable concentrations basic elements of cell division are on the impact of tuniclike psammosilene root adventive root biomass
Be inoculated in by tuniclike psammosilene root adventive root and be attached with in the MS solid culture medium of the variable concentrations basic element of cell division, observing and measuring its adventive root biomass increases situation.
The impact that the table 4 variable concentrations basic element of cell division grows tuniclike psammosilene root adventive root
Adventive root is attached with adventive root fresh weight in the MS medium of 1.0mg/LKT, 0.01mg/L6-BA and 0.001mg/LTDZ all higher after cultivating 40d, and without significant difference between three; In the medium of additional 0.3mg/L6-BA, adventive root fresh weight is minimum, is only 0.619g; In the medium of MS+1.0mg/LKT, adventive root dry weight is the highest, is 0.518g, and processes equal significant difference with other.And the minimum medium of gained dry weight is MS+0.3mg/L6-BA, in this medium, the dry weight of adventive root is only 0.094g.
In additional 1.0mg/LKT medium, obtain adventive root dry rate (table 4) of higher level, and adventive root growth better (Fig. 4).Therefore, in the solid culture medium of MS+1.0mg/LKT+30g/L white granulated sugar, the growth of tuniclike psammosilene root adventive root better.Calculate with dry weight 0.518g/25mL, the dry weight of the tuniclike psammosilene root adventive root that MS solid culture medium obtains is 20.72gL
-1, be document " affect the factor of tuniclike psammosilene root growth of hair root and saponin(e accumulation " (Tian Sidi etc., time precious traditional Chinese medical science traditional Chinese medicines [J], 2012,23 (4): 824-826.) report 1.52 times.
(note: according to above document, its 1/2MS+0.5mg/LIBA dry weight yield is the highest, is 13.67g/L.Compare with the present invention, in the solid culture medium of MS+1.0mg/LKT, the every 25mL of its dry weight is 0.518g, then the dry weight of 1L is 0.518g/25mL × (40 × 25mL)=20.72g, therefore the ratio of the present invention and document dry weight yield: 20.72g/13.67g=1.52 doubly.）
2.5 hormon proportionings are on the impact of tuniclike psammosilene root adventive root biomass
Tuniclike psammosilene root adventive root is inoculated in the solid culture medium containing different growth hormone and basic element of cell division proportioning and cultivates, and it the results are shown in table 5.
The impact that table 5 different culture media scheme grows tuniclike psammosilene root adventive root
As can be seen from Table 5,40d after incubation, there is certain difference in the dry weight of 8 combinations, what wherein dry weight was minimum is combination MS+0.01mg/LTDZ+0.05mg/LNAA+30g/L white granulated sugar, and its dry weight is only 0.224g; And combine MS+0.5mg/LNAA+0.5mg/LKT+30g/L white granulated sugar, and its dry weight is 0.474g(Fig. 5), be the maximum of 8 combinations, but be all less than the output in MS+1.0mg/LKT+30g/L white granulated sugar and 1.0mg/LIBA+30g/L white granulated sugar.
Claims (5)
1. a method for tuniclike psammosilene root adventive root fast breeding cultivation, comprising:
(1) terminal bud cutting tuniclike psammosilene root aseptic seedling is inoculated in the root media of 1/2MS+0.3mg/LIBA+0.1mg/LNAA+0.3g/L active carbon, forms the indefinite network of roots of hair shape after 60d;
(2) by block mode cut expand numerous after the indefinite network of roots of tuniclike psammosilene root, be inoculated in and with the addition of in the MS solid-based basal culture medium of hormone and white granulated sugar, the medium of every bottle of packing 25mL, every bottle graft kind 4 pieces of indefinite networks of roots of tuniclike psammosilene root.
2. method according to claim 1, is characterized in that: described medium is following one:
(1) combination of MS solid-based basal culture medium and growth hormone: MS+30g/L white granulated sugar+1.0mg/LIBA;
(2) combination of MS solid-based basal culture medium and the basic element of cell division: MS+30g/L white granulated sugar+1.0mg/LKT;
(1) ~ (2) condition of culture is above: 25 ± 2 DEG C, light application time 12h/d, intensity of illumination 1500 ~ 2000lx, incubation time 40d.
3. method according to claim 1 and 2, is characterized in that: described medium is the combination of MS solid-based basal culture medium and the basic element of cell division: MS+1.0mg/LKT.
4. method according to claim 1 and 2, is characterized in that: the tuniclike psammosilene root adventive root tissue block being inoculated in described medium is 0.05g/ block, and this tissue block dry weight is roughly equal to 0.0056 ± 0.0004g/ block.
5. method according to claim 3, is characterized in that: the tuniclike psammosilene root adventive root tissue block being inoculated in described medium is 0.05g/ block, and this tissue block dry weight is roughly equal to 0.0056 ± 0.0004g/ block.
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| CN109906941A (en) * | 2019-04-22 | 2019-06-21 | 贵州省生物研究所 | A kind of method of tuniclike psammosilene root adventitious root suspension culture |
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| CN102771397A (en) * | 2012-08-17 | 2012-11-14 | 成都市三禾田生物技术有限公司 | Method for establishing adventitious root cultivation system of Psammosilene tuniceoides W. C. Wu et C. Y. Wu and expanding cultivation method of Psammosilene tuniceoides W. C. Wu et C. Y. Wu |
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| CN102771397A (en) * | 2012-08-17 | 2012-11-14 | 成都市三禾田生物技术有限公司 | Method for establishing adventitious root cultivation system of Psammosilene tuniceoides W. C. Wu et C. Y. Wu and expanding cultivation method of Psammosilene tuniceoides W. C. Wu et C. Y. Wu |
Non-Patent Citations (3)
| Title |
|---|
| 邱运亮: "《植物组培快繁技术》", 28 February 2010 * |
| 郑永娟: "《植物组织培养》", 29 February 2012 * |
| 钱子刚: "《药用植物组织培养》", 31 January 2007 * |
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| CN109906941A (en) * | 2019-04-22 | 2019-06-21 | 贵州省生物研究所 | A kind of method of tuniclike psammosilene root adventitious root suspension culture |
| CN109906941B (en) * | 2019-04-22 | 2022-12-23 | 贵州省生物研究所 | Psammosilene tunicoides adventitious root suspension culture method |
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