CN105420113B - Based on the water supply of capillary power for the microalgae solid phase apposition growth cultural method of nutrition - Google Patents
Based on the water supply of capillary power for the microalgae solid phase apposition growth cultural method of nutrition Download PDFInfo
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- 235000016709 nutrition Nutrition 0.000 title claims abstract description 17
- 230000035764 nutrition Effects 0.000 title claims abstract description 17
- 241000195493 Cryptophyta Species 0.000 claims abstract description 21
- 235000015097 nutrients Nutrition 0.000 claims abstract description 20
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- 238000003306 harvesting Methods 0.000 claims abstract description 14
- 241000195663 Scenedesmus Species 0.000 claims abstract description 4
- 241000883966 Astrophytum capricorne Species 0.000 claims abstract description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 3
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- 239000007788 liquid Substances 0.000 claims description 3
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- 229910021380 Manganese Chloride Inorganic materials 0.000 claims 1
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- UFMZWBIQTDUYBN-UHFFFAOYSA-N cobalt(II) nitrate Inorganic materials [Co+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O UFMZWBIQTDUYBN-UHFFFAOYSA-N 0.000 claims 1
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- Cultivation Of Seaweed (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of microalgae solid phase apposition growth cultural methods to be supplied water based on capillary power for nutrition for the production harvest technical field for belonging to microalgae.Algae solution is inoculated on solid phase carrier by the cultural method, and solid phase carrier is placed in transparent support system, it then using the capillary performance automatic sucking BG11 fluid nutrient medium of solid phase carrier and is stored in solid phase carrier, supplies water the microalgae on solid phase carrier for nutrition, realize the growth of microalgae；The microalgae is scenedesmus, ball algae or goat's horn crescent moon algae.The culture that microalgae is carried out using the present invention, can simplify culture and harvest technology, save the energy, can be applied to energy microalgae, edible mushroom, in the industrial production of the products such as microalgae biological products.
Description
Technical field
The invention belongs to the productions of microalgae to harvest technical field, and in particular to a kind of to be supplied water based on capillary power for nutrition
Microalgae solid phase apposition growth cultural method.
Background technique
Certain microalgae cells include high proportion grease, biodiesel can be produced by rouge transformation, as renewable energy
Source replaces traditional non-renewable energy resources such as coal and petroleum, alleviates global energy crisis.Simultaneously during microalgae is mass produced,
Microalgae absorbs the CO in atmosphere2, to reduce atmospheric greenhouse gas content.Some microalgae cells, because its contain in vivo it is higher
Protein and nutriment, so edible value with higher, such as spirulina.In addition, some microalgae cells, such as rain
Raw haematococcus has medical value in stationary phase energy synthesizing astaxanthin.It is certain to realize that the heavy industrialization culture of microalgae has
Economic value and the ecological value.
Most of microalgae cell is small in size, and size is about 3-10 μm.In microdisk electrode, it is system that microalgae, which collects concentration process,
About one of the critical issue of its heavy industrialization application.Existing microalgae harvesting method has filtering, air bearing, centrifugation and sedimentation
Deng, however these methods have certain problems.Filtering be easy to cause filter net jam, and collection efficiency is lower, increases pressure
Microalgae harvesting cost and energy consumption can be then increased, while increasing the requirement of mechanical strength to strainer；Centrifugal separation can efficiently separate
Microalgae, but energy consumption needed for this method is very big, it is at high cost, to influence the extensive industrial application of this method, high speed centrifugation side
Method is also possible to destruction cell wall and even results in clasmatosis；Gas floatation process is a kind of more potential microalgae concentration method, but the method needs
There are reasonable air-flotation separation equipment and separating technology, separating effect is influenced by factors such as device structure and process conditions；It is heavy
Drop is to make microalgae that flocculating setting occur, but coagulant draws by adjusting pH or adding inorganic or organic compound to culture solution
Enter will affect microalgae quality, causes the processing of microalgae subsequent applications impacted.
In addition to harvesting difficulty, to a large amount of consumption of water resource in microdisk electrode, and its heavy industrialization application is restricted
One of critical issue.According to existing evaluation of life cycle, in using microalgae production biodiesel process, average every production
The biodiesel of 1kg needs to consume 3726kg fresh water, although by the recycling to moisture in technical process, water needed for harvesting link
Most of saving can be obtained in amount, but the fresh water demand of cultivation stage is then rigid demand in Traditional liquid phase culture, can not be saved.
Summary of the invention
In order to solve the problems in the existing technology, the purpose of the present invention is to provide one kind is supplied water based on capillary power
For the microalgae solid phase apposition growth cultural method of nutrition, continuous, convenient, the economic and large-scale culture and receipts of microalgae are realized
It obtains.
To achieve the goals above, The technical solution adopted by the invention is as follows:
A kind of microalgae solid phase apposition growth cultural method to be supplied water based on capillary power for nutrition, the cultural method is by algae
Liquid is inoculated on solid phase carrier, and solid phase carrier is placed in support system, then automatic using the capillary performance of solid phase carrier
It draws BG11 fluid nutrient medium and is stored in solid phase carrier, supply water the microalgae on solid phase carrier for nutrition, realize microalgae
Growth；
The microalgae is scenedesmus, ball algae or goat's horn crescent moon algae；
The constituent of the BG11 fluid nutrient medium are as follows: 1500mg.L-1NaNO3, 40mg.L-1K2HPO4.3H2O,
75mg.L-1MgSO4.7H2O, 36mg.L-1CaCl2.2H2O, 6mg.L-1Citric acid, 20mg.L-1Na2CO3, 1mg.L-1Na2EDTA,
2.86mg.L-1H3BO3, 1.81mg.L-1MnCl2.4H2O, 0.22mg.L-1ZnSO4.7H2O, 0.079mg.L-1CuSO4.5H2O,
0.39mg.L-1Na2MoO4.2H2O, 0.049mg.L-1Co(NO3)2.6H2O。
The inoculum concentration of the microalgae is 0.2-0.3 grams/m.
The growth conditions of the microalgae are as follows: intensity of illumination 1000-1500lux, Light To Dark Ratio 14h:10h, humidity 25-
35%, temperature is 23-27 DEG C.
The transportation power of the BG11 fluid nutrient medium is the capillary power of solid phase carrier, passes through capillary power supply
BG11 fluid nutrient medium is equal to the evaporation consumption of BG11 fluid nutrient medium on solid phase carrier.
The shape of the solid phase carrier is blanket or pencil.
The material of the solid phase carrier is fiber or cotton, open structure and stronger capillary with high-specific surface area
Phenomenon and storage capacity.
The modes of emplacement of the solid phase carrier is that level lays flat, vertically places vertically or tiltedly puts in parallel, and is inoculated on solid phase carrier
The extreme lower position of microalgae cell is higher than the highest liquid level of BG11 fluid nutrient medium, prevent BG11 fluid nutrient medium refluence or
Person's microalgae cell is dissolved in BG11 fluid nutrient medium.
The support system of the solid phase carrier is transparent support system, guarantees the double-side photic of solid phase carrier, releases light suppression
System.
The growth pattern of the microalgae includes two kinds, is respectively attached directly on solid phase carrier with the growth of algae form membrane
Mode disperses the mode of growth with being distributed in the storage moisture of solid phase carrier hole.
The solid phase carrier can recycle, and using the microalgae not removed on solid phase carrier as seed, realize micro-
The continuous culture of algae and harvest.
The cultural method can be used in producing the metabolite of microalgae or microalgae.
Advantages of the present invention are as follows:
1) energy is saved.The harvest process of Traditional liquid phase algal biomass is often carried out using modes such as centrifugation, precipitatings, to electricity
Power consumption is big or hydraulic detention time is longer.Using this method culture microalgae cell, the enrichment concentration process of frustule can be saved,
Carrier can enter extraction link after drying, save a large amount of resource and the energy.
2) water resource and nitrogen and phosphorus source are saved.Microalgae cell largely is completed to give birth in a manner of algae film in the cultural method
It is long, for dispersing the Free water of frustule when saving Liquid Culture；And incubation is nearly free from spent media, avoids
The waste of nitrogen and phosphorous nutrient.
3) space is saved.In Traditional liquid phase culture microalgae method, the limitation being illuminated by the light, bioreactor often depth as shallow
And floor space is big, occupies more space resource.It, can be on space structure by solid phase when carrying out microalgae cell culture using this method
Space is saved in the accumulation of carrier pile.
Detailed description of the invention
Fig. 1 is the process schematic diagram of solid phase carrier.
Fig. 2 is a kind of microalgae solid phase apposition growth cultural method process flow chart to be supplied water based on capillary power for nutrition.
Fig. 3 is two kinds of capillary power bioreactor schematic diagrames.
Fig. 4 is the microalgae total amount versus time curve figure using cultural method of the present invention.
Specific embodiment
Below in conjunction with drawings and the specific embodiments, the present invention is described in further detail.
The preparation of solid phase carrier: being compared by the performance to multiple material, and selection processes bunchy by model terylene 300D
Superfine fibre (as shown in Figure 1), in the organic glass algae box of laboratory tile place.With the terylene 300D superfine fibre of 5cm
As capillary drain, in an access BG11 fluid nutrient medium, other end is contacted with solid phase carrier.If large-scale production is answered
With then needing for large area solid phase carrier to be divided into several area solid phase carriers appropriate, every solid phase carrier need to be used individually
Capillary drain is introduced into BG11 fluid nutrient medium.
Cultivation stage: the solid phase carrier for being inoculated with upper microalgae is cultivated under illumination condition to can harvest the stage.Harvest time
Depending on cultivating purpose.Grease in frustule is such as extracted, then is harvested in stationary phase mid-term.
The harvest stage: the carrier for adhering to a large amount of frustules is separated into microalgae biomass by mechanically decoupled technique and blank carries
Body, the part microalgae cell not being removed are re-used as algae, introduce culture medium by capillary drain and continue to cultivate.By with
Upper type, Reasonable Regulation And Control harvest frequency and harvest ratio, realize continuous, convenient, economic microalgae large-scale culture.The culture side
The process flow chart of method is as shown in Figure 2.
Embodiment 1: small-scale interval microalgae culture method
Scenedesmus Scenedesmus.LX1 0.267g/m is uniformly inoculated on the superfine fibre carrier of 3cm × 5cm2.Every
Prepared BG11 fluid nutrient medium 30ml is poured into a glass culture dish, uses the superfine fibre Shu Liantong solid phase carrier of 5cm
With BG11 fluid nutrient medium, after 10-20 minutes, BG11 fluid nutrient medium can infiltrate entire algae blanket through capillary action.By algae
It is 25 ± 2 DEG C that blanket and culture medium, which are placed in temperature, humidity 30%, intensity of illumination 1100lux, and Light To Dark Ratio is the artificial of 14:10
In weather incubator.During culture, the method for adding culture medium by interval guarantees there is remaining culture medium always in glass dish.
Culture terminates, and every algae blanket water is between 215mL-235mL.In addition, to guarantee that algae blanket is evenly illuminated by the light, just by algae blanket daily
Reverse side is exchanged once.After culture 18 days, liquid phase algae density is up to 67.6g/m2, converting into liquid phase output is 0.54g/L or so, such as
Shown in Fig. 4.
Claims (7)
1. a kind of microalgae solid phase apposition growth cultural method to be supplied water based on capillary power for nutrition, which is characterized in that the training
Algae solution is inoculated on solid phase carrier by the method for supporting, and solid phase carrier is placed in support system, then utilizes the hair of solid phase carrier
Thin performance automatic sucking BG11 fluid nutrient medium is simultaneously stored in solid phase carrier, supplies water the microalgae on solid phase carrier for nutrition,
Realize the growth of microalgae；
The solid phase carrier is that terylene 300D processes bundles of superfine fibre；
The transportation power of the BG11 fluid nutrient medium is the capillary power of solid phase carrier, is made with the superfine fibre beam of solid phase carrier
For capillary drain, in an access BG11 fluid nutrient medium, other end is contacted with solid phase carrier；
The growth pattern of the microalgae includes two kinds, is respectively attached directly on solid phase carrier in a manner of the growth of algae form membrane
Be distributed in disperse in the storage moisture of solid phase carrier hole growth mode；
The microalgae is scenedesmus, ball algae or goat's horn crescent moon algae；
The constituent of the BG11 fluid nutrient medium are as follows: 1500mgL-1NaNO3, 40mgL-1K2HPO4·3H2O, 75mg
L-1MgSO4·7H2O, 36mgL-1CaCl2·2H2O, 6mgL-1Citric acid, 20mgL-1Na2CO3, 1mgL-1Na2EDTA,
2.86mg·L-1H3BO3, 1.81mgL-1MnCl2·4H2O, 0.22mgL-1ZnSO4·7H2O, 0.079mgL-1CuSO4·
5H2O, 0.39mgL-1Na2MoO4·2H2O, 0.049mgL-1Co(NO3)2·6H2O。
2. a kind of microalgae solid phase apposition growth culture side to be supplied water based on capillary power for nutrition according to claim 1
Method, which is characterized in that the inoculum concentration of the microalgae is 0.2-0.3 grams/m.
3. a kind of microalgae solid phase apposition growth culture side to be supplied water based on capillary power for nutrition according to claim 1
Method, which is characterized in that the growth conditions of the microalgae are as follows: intensity of illumination 1000-1500lux, Light To Dark Ratio 14h:10h are wet
Degree is 25-35%, and temperature is 23-27 DEG C.
4. a kind of microalgae solid phase apposition growth culture side to be supplied water based on capillary power for nutrition according to claim 1
Method, which is characterized in that the modes of emplacement of the solid phase carrier lays flat, vertically places vertically or tiltedly puts in parallel for level, and solid phase carrier
The extreme lower position of upper inoculation microalgae is higher than the highest liquid level of BG11 fluid nutrient medium.
5. a kind of microalgae solid phase apposition growth culture side to be supplied water based on capillary power for nutrition according to claim 1
Method, which is characterized in that the support system of the solid phase carrier is transparent support system.
6. a kind of microalgae solid phase apposition growth culture side to be supplied water based on capillary power for nutrition according to claim 1
Method, which is characterized in that the solid phase carrier can recycle, using the microalgae not removed on solid phase carrier as seed,
Realize the continuous culture and harvest of microalgae.
7. a kind of microalgae solid phase apposition growth culture supplied water based on capillary power for nutrition described in any one of claims 1-6
Method, which is characterized in that the cultural method can be used in producing the metabolite of microalgae or microalgae.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001238672A (en) * | 2000-02-29 | 2001-09-04 | Hitachi Chem Co Ltd | Laminate-like microbial carrier |
| JP2003005626A (en) * | 2001-06-20 | 2003-01-08 | Kato Construction Co Ltd | Biotope kit for environment study |
| CN103409321A (en) * | 2013-07-26 | 2013-11-27 | 清华大学 | Microalgae suspension-adhesion mixed culture and separated harvesting method based on suspended carrier |
-
2015
- 2015-11-17 CN CN201510792309.3A patent/CN105420113B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001238672A (en) * | 2000-02-29 | 2001-09-04 | Hitachi Chem Co Ltd | Laminate-like microbial carrier |
| JP2003005626A (en) * | 2001-06-20 | 2003-01-08 | Kato Construction Co Ltd | Biotope kit for environment study |
| CN103409321A (en) * | 2013-07-26 | 2013-11-27 | 清华大学 | Microalgae suspension-adhesion mixed culture and separated harvesting method based on suspended carrier |
Non-Patent Citations (2)
| Title |
|---|
| Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: an experimental study;Jing Shi et al;《J.Appl Phycol》;20071231;第19卷;摘要，图1，第418页右栏第2段 |
| 微藻规模培养技术研究进展;刘天中 等;《生命科学》;20140531;第26卷(第5期);第2.1.2节 |
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