CN105418792A - Method for high efficiently extracting pectin from banana peels - Google Patents
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- CN105418792A CN105418792A CN201511022906.4A CN201511022906A CN105418792A CN 105418792 A CN105418792 A CN 105418792A CN 201511022906 A CN201511022906 A CN 201511022906A CN 105418792 A CN105418792 A CN 105418792A
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
- C08B37/0048—Processes of extraction from organic materials
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Abstract
The invention discloses a method for high efficiently extracting pectin from banana peels. According to the method, the optimal technical parameters are as follows: ultrasonic power 250W, material/liquid ratio 35:1 mL/g, extraction time 65 minutes, extraction temperature 65 DEG C, and concentration of extractant (ammonium oxalate) 50%. The extraction rate of pectin is 25%. The extraction rate is high, the purity of pectin is high, and the comprehensive utilization rate of banana is improved. The extraction method has the advantages of simpleness, easy operation, and low cost.
Description
Technical field
The present invention relates to the extracting method of pectin, be specifically related to the method for pectin in a kind of high efficiency extraction Pericarpium Musae.
Background technology
Pectin is the complex polysaccharide relevant to plant cell wall.It comprises inserts and the polygalacturonic acid main chain be connected with the α 1-4 key of ferulic modification with sugarless components such as ethanoyl, methyl with tral sugar side chains with rhamnosyl residue.The tral sugar side chains comprising arabinan and arabogalactan is attached on the rhamnosyl residue in main chain.And containing a large amount of food fibres such as pectin, Mierocrystalline cellulose in any of several broadleaf plants skin, but throw away as waste, if be not utilized, the serious waste of resource can be caused, Pericarpium Musae contains a large amount of organism simultaneously, is dropped rear very difficult degraded and causes environmental pollution.In Pericarpium Musae, pectin content is higher, extracts if carry out and is used, and not only economizes on resources but also cut the waste, and will provide new way, improve the economic benefit of banana added value and banana processing enterprise for banana comprehesive utilizes.At present, the whole world every year to the demand of pectin at more than 30000t, and to increase with the speed of 15% every year.China's pectin plant productivity only 200 ~ 300t, wherein about has 80% to need import.
In recent years, about the method extracting Pectin from Banana Peel mainly contains ethanol precipitation, salting-out process, ion exchange method etc. in research report, extraction yield is generally not high.Oxalate denominationby in ammonium oxalate can generate solubility ammonium salt with the calcium ion chelating in calcium pectinate, increase pectolysis, good application is obtained in the fruits and vegetables such as Herba Passiflorae Caeruleae, Radix Dauci Sativae pectin extraction, but extraction time is longer needed for the method, and Extracting temperature is higher, easily cause hydrolyzed pectin, extraction process needs to improve; Ultrasonic wave can make pectin and each Component seperation of cell walls, and destroying cell walls can reduce Extracting temperature, shortens extraction time, contributes to the extraction of pectin.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide that a kind of cost is low, method is simple, the method for pectin in easy and simple to handle, highly purified high efficiency extraction Pericarpium Musae.
In order to achieve the above object, the technical scheme that the present invention takes is:
A method for pectin in high efficiency extraction Pericarpium Musae, comprises the following steps:
(1) pre-treatment: get Pericarpium Musae and be placed in distilled water immersion 20 minutes, be cut into small pieces after removal of impurities, then put into after soaking 15min containing 0.4% sodium bisulfite, 0.3% citric acid, 0.4% sodium-chlor mixing solutions, to go out enzyme 5 ~ 10min with 100 DEG C of water vapors, warm water rinse 2 ~ 3 times are used after cooling, the oven drying of 60 DEG C is placed in again with gauze extruding, for subsequent use after pulverizing;
(2) supersound extraction: the Pericarpium Musae after pulverizing is placed in beaker, be add 30:1 ~ 40:1ml/g to add the ammonium oxalate solution that concentration is 0.4 ~ 1.2% by solid-liquid ratio, be placed in that power is 250W, temperature is after the supersound extraction 60 ~ 70min of 60 ~ 70 DEG C, vacuum filtration, discard filter residue, collect filtrate and its pH is adjusted to 3.5 ~ 4.0;
(3) decolour: add after gac is placed in the water bath heat preservation 10min of 60 DEG C in pectin extracting soln, be placed in the centrifugal 10min of whizzer that rotating speed is 5000r/min, collect filtrate;
(4) concentrated: it is 1/2nd of original volume that the extracting solution after decolouring is placed in 100 DEG C of water-bath evaporation concentration to volume;
(5) alcohol settling: by the cooling of concentrated extracting solution, be incorporated as 95% alcohol steep of original volume 0.8 ~ 1.5 times precooling while stirring, then the centrifugal 20min of whizzer of 5000r/min is placed in, collecting precipitation, be placed on for 2 times in 60 DEG C of baking ovens with the absolute ethanol washing with concentrated solution same volume and dry, can pectin be obtained.
As optimal technical scheme, in order to ensure that the pectin in Pericarpium Musae is all dissolved in ammonium oxalate, to be conducive to the extraction yield and the purity that improve pectin, described step (2) mesoxalic acid ammonium preferably concentration is 0.8%.
As optimal technical scheme, in order to ensure that the pectin in Pericarpium Musae is all dissolved in ammonium oxalate, to be conducive to the extraction yield and the purity that improve pectin, in described step (2), better supersound extraction temperature is 65 DEG C.
As optimal technical scheme, in order to ensure that the pectin in Pericarpium Musae is all dissolved in ammonium oxalate, to be conducive to the extraction yield and the purity that improve pectin, described step (2) mesoxalic acid ammonium and Pericarpium Musae preferably solid-liquid ratio are 35:1ml/g.
As optimal technical scheme, in order to ensure that the pectin in Pericarpium Musae is all dissolved in ammonium oxalate, to be conducive to the extraction yield and the purity that improve pectin, in described step (2), the better supersound extraction time is 65min.
Beneficial effect of the present invention: extracting method of the present invention is simple, easy handling, extraction cost are low, under the condition of ultrasonic power 250W, solid-liquid ratio 35:1ml/g, extraction time be 65min, Extracting temperature is 65 DEG C, the concentration of extraction agent ammonium oxalate is the optimum extraction process of 50%, pectin yield is 25%, extraction yield and the purity of pectin are higher, improve the comprehensive utilization ratio of banana.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
A method for pectin in high efficiency extraction Pericarpium Musae, comprises the following steps:
(1) pre-treatment: get Pericarpium Musae and be placed in distilled water immersion 20 minutes, be cut into small pieces after removal of impurities, then put into after soaking 15min containing 0.4% sodium bisulfite, 0.3% citric acid, 0.4% sodium-chlor mixing solutions, to go out enzyme 5min with 100 DEG C of water vapors, warm water rinse 2 times are used after cooling, the oven drying of 60 DEG C is placed in again with gauze extruding, for subsequent use after pulverizing;
(2) supersound extraction: the Pericarpium Musae after pulverizing is placed in beaker, be add 30:1ml/g to add the ammonium oxalate solution that concentration is 0.4% by solid-liquid ratio, be placed in that power is 250W, temperature is after the supersound extraction 60min of 60 DEG C, vacuum filtration, discard filter residue, collect filtrate and its pH is adjusted to 3.5;
(3) decolour: add after gac is placed in the water bath heat preservation 10min of 60 DEG C in pectin extracting soln, be placed in the centrifugal 10min of whizzer that rotating speed is 5000r/min, collect filtrate;
(4) concentrated: it is 1/2nd of original volume that the extracting solution after decolouring is placed in 100 DEG C of water-bath evaporation concentration to volume;
(5) alcohol settling: by the cooling of concentrated extracting solution, be incorporated as 95% alcohol steep of original volume 0.8 doubly precooling while stirring, then the centrifugal 20min of whizzer of 5000r/min is placed in, collecting precipitation, be placed on for 2 times in 60 DEG C of baking ovens with the absolute ethanol washing with concentrated solution same volume and dry, obtaining extraction yield is the pectin of 24.67%.
Embodiment 2
A method for pectin in high efficiency extraction Pericarpium Musae, comprises the following steps:
(1) pre-treatment: get Pericarpium Musae and be placed in distilled water immersion 20 minutes, be cut into small pieces after removal of impurities, then put into after soaking 15min containing 0.4% sodium bisulfite, 0.3% citric acid, 0.4% sodium-chlor mixing solutions, to go out enzyme 7min with 100 DEG C of water vapors, warm water rinse 73 times are used after cooling, the oven drying of 60 DEG C is placed in again with gauze extruding, for subsequent use after pulverizing;
(2) supersound extraction: the Pericarpium Musae after pulverizing is placed in beaker, be add 32ml/g to add the ammonium oxalate solution that concentration is 0.6% by solid-liquid ratio, be placed in that power is 250W, temperature is after the supersound extraction 62min of 62 DEG C, vacuum filtration, discard filter residue, collect filtrate and its pH is adjusted to 3.6;
(3) decolour: add after gac is placed in the water bath heat preservation 10min of 60 DEG C in pectin extracting soln, be placed in the centrifugal 10min of whizzer that rotating speed is 5000r/min, collect filtrate;
(4) concentrated: it is 1/2nd of original volume that the extracting solution after decolouring is placed in 100 DEG C of water-bath evaporation concentration to volume;
(5) alcohol settling: by the cooling of concentrated extracting solution, be incorporated as 95% alcohol steep of original volume 1.0 doubly precooling while stirring, then the centrifugal 20min of whizzer of 5000r/min is placed in, collecting precipitation, be placed on for 2 times in 60 DEG C of baking ovens with the absolute ethanol washing with concentrated solution same volume and dry, the pectin that extraction yield is 24.9% can be obtained.
Embodiment 3
A method for pectin in high efficiency extraction Pericarpium Musae, comprises the following steps:
(1) pre-treatment: get Pericarpium Musae and be placed in distilled water immersion 20 minutes, be cut into small pieces after removal of impurities, then put into after soaking 15min containing 0.4% sodium bisulfite, 0.3% citric acid, 0.4% sodium-chlor mixing solutions, to go out enzyme 8min with 100 DEG C of water vapors, warm water rinse 3 times are used after cooling, the oven drying of 60 DEG C is placed in again with gauze extruding, for subsequent use after pulverizing;
(2) supersound extraction: the Pericarpium Musae after pulverizing is placed in beaker, be add 35:1ml/g to add the ammonium oxalate solution that concentration is 0.8% by solid-liquid ratio, be placed in that power is 250W, temperature is after the supersound extraction 65min of 65 DEG C, vacuum filtration, discard filter residue, collect filtrate and its pH is adjusted to 3.8;
(3) decolour: add after gac is placed in the water bath heat preservation 10min of 60 DEG C in pectin extracting soln, be placed in the centrifugal 10min of whizzer that rotating speed is 5000r/min, collect filtrate;
(4) concentrated: it is 1/2nd of original volume that the extracting solution after decolouring is placed in 100 DEG C of water-bath evaporation concentration to volume;
(5) alcohol settling: by the cooling of concentrated extracting solution, be incorporated as 95% alcohol steep of original volume 1.2 doubly precooling while stirring, then the centrifugal 20min of whizzer of 5000r/min is placed in, collecting precipitation, be placed on for 2 times in 60 DEG C of baking ovens with the absolute ethanol washing with concentrated solution same volume and dry, obtaining extraction yield is the pectin of 25%.
Embodiment 4
A method for pectin in high efficiency extraction Pericarpium Musae, comprises the following steps:
(1) pre-treatment: get Pericarpium Musae and be placed in distilled water immersion 20 minutes, be cut into small pieces after removal of impurities, then put into after soaking 15min containing 0.4% sodium bisulfite, 0.3% citric acid, 0.4% sodium-chlor mixing solutions, to go out enzyme 9min with 100 DEG C of water vapors, warm water rinse 3 times are used after cooling, the oven drying of 60 DEG C is placed in again with gauze extruding, for subsequent use after pulverizing;
(2) supersound extraction: the Pericarpium Musae after pulverizing is placed in beaker, be add 38:1ml/g to add the ammonium oxalate solution that concentration is 1.0% by solid-liquid ratio, be placed in that power is 250W, temperature is after the supersound extraction 68min of 68 DEG C, vacuum filtration, discard filter residue, collect filtrate and its pH is adjusted to 3.9;
(3) decolour: add after gac is placed in the water bath heat preservation 10min of 60 DEG C in pectin extracting soln, be placed in the centrifugal 10min of whizzer that rotating speed is 5000r/min, collect filtrate;
(4) concentrated: it is 1/2nd of original volume that the extracting solution after decolouring is placed in 100 DEG C of water-bath evaporation concentration to volume;
(5) alcohol settling: by the cooling of concentrated extracting solution, be incorporated as 95% alcohol steep of original volume 1.4 doubly precooling while stirring, then the centrifugal 20min of whizzer of 5000r/min is placed in, collecting precipitation, be placed on for 2 times in 60 DEG C of baking ovens with the absolute ethanol washing with concentrated solution same volume and dry, obtaining extraction yield is the pectin of 24.7%.
Embodiment 5
A method for pectin in high efficiency extraction Pericarpium Musae, comprises the following steps:
(1) pre-treatment: get Pericarpium Musae and be placed in distilled water immersion 20 minutes, be cut into small pieces after removal of impurities, then put into after soaking 15min containing 0.4% sodium bisulfite, 0.3% citric acid, 0.4% sodium-chlor mixing solutions, to go out enzyme 10min with 100 DEG C of water vapors, warm water rinse 3 times are used after cooling, the oven drying of 60 DEG C is placed in again with gauze extruding, for subsequent use after pulverizing;
(2) supersound extraction: the Pericarpium Musae after pulverizing is placed in beaker, be add 40:1ml/g to add the ammonium oxalate solution that concentration is 1.2% by solid-liquid ratio, be placed in that power is 250W, temperature is after the supersound extraction 70min of 70 DEG C, vacuum filtration, discard filter residue, collect filtrate and its pH is adjusted to 4.0;
(3) decolour: add after gac is placed in the water bath heat preservation 10min of 60 DEG C in pectin extracting soln, be placed in the centrifugal 10min of whizzer that rotating speed is 5000r/min, collect filtrate;
(4) concentrated: it is 1/2nd of original volume that the extracting solution after decolouring is placed in 100 DEG C of water-bath evaporation concentration to volume;
(5) alcohol settling: by the cooling of concentrated extracting solution, be incorporated as 95% alcohol steep of original volume 1.5 doubly precooling while stirring, then the centrifugal 20min of whizzer of 5000r/min is placed in, collecting precipitation, be placed on for 2 times in 60 DEG C of baking ovens with the absolute ethanol washing with concentrated solution same volume and dry, obtaining extraction yield is the pectin of 24.5%.
It should be pointed out that above case is only the representational example of the present invention.Obviously, technical scheme of the present invention is not limited to above-described embodiment, can also have many sex change.
Claims (5)
1. the method for pectin in high efficiency extraction Pericarpium Musae, is characterized in that, comprise the following steps:
(1) pre-treatment: get Pericarpium Musae and be placed in distilled water immersion 20 minutes, be cut into small pieces after removal of impurities, then put into after soaking 15min containing 0.4% sodium bisulfite, 0.3% citric acid, 0.4% sodium-chlor mixing solutions, to go out enzyme 5 ~ 10min with 100 DEG C of water vapors, warm water rinse 2 ~ 3 times are used after cooling, the oven drying of 60 DEG C is placed in again with gauze extruding, for subsequent use after pulverizing;
(2) supersound extraction: the Pericarpium Musae after pulverizing is placed in beaker, be add 30:1 ~ 40:1ml/g to add the ammonium oxalate solution that concentration is 0.4 ~ 1.2% by solid-liquid ratio, be placed in that power is 250W, temperature is after the supersound extraction 60 ~ 70min of 60 ~ 70 DEG C, vacuum filtration, discard filter residue, collect filtrate and its pH is adjusted to 3.5 ~ 4.0;
(3) decolour: add after gac is placed in the water bath heat preservation 10min of 60 DEG C in pectin extracting soln, be placed in the centrifugal 10min of whizzer that rotating speed is 5000r/min, collect filtrate;
(4) concentrated: it is 1/2nd of original volume that the extracting solution after decolouring is placed in 100 DEG C of water-bath evaporation concentration to volume;
(5) alcohol settling: by the cooling of concentrated extracting solution, be incorporated as 95% alcohol steep of original volume 0.8 ~ 1.5 times precooling while stirring, then the centrifugal 20min of whizzer of 5000r/min is placed in, collecting precipitation, be placed on for 2 times in 60 DEG C of baking ovens with the absolute ethanol washing with concentrated solution same volume and dry, can pectin be obtained.
2. a kind of method of pectin in high efficiency extraction Pericarpium Musae according to claim 1, is characterized in that: described step (2) mesoxalic acid ammonium preferably concentration is 0.8%.
3. a kind of method of pectin in high efficiency extraction Pericarpium Musae according to claim 2, is characterized in that: in described step (2), better supersound extraction temperature is 65 DEG C.
4. a kind of method of pectin in high efficiency extraction Pericarpium Musae according to claim 3, is characterized in that: described step (2) mesoxalic acid ammonium and Pericarpium Musae preferably solid-liquid ratio are 35:1ml/g.
5. a kind of method of pectin in high efficiency extraction Pericarpium Musae according to claim 4, is characterized in that: in described step (2), the better supersound extraction time is 65min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108567143A (en) * | 2018-03-28 | 2018-09-25 | 广西玉阳绿州生物科技有限公司 | Lowiaceae plant extraction liquid, production method and its application in treatment of cancer |
| CN111500656A (en) * | 2020-04-14 | 2020-08-07 | 广西农业职业技术学院 | Method for extracting low-molecular-weight pectin from banana peel |
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| JPS6465101A (en) * | 1987-09-05 | 1989-03-10 | Takeshi Ishii | Water hyacinth pectin |
| CN101148482A (en) * | 2007-11-05 | 2008-03-26 | 桂林工学院 | Method for extracting pectin from banana peel |
| CN104031172A (en) * | 2014-06-03 | 2014-09-10 | 沈阳师范大学 | Method for extracting pectin from banana peel by ultrasonic synergistic ammonium oxalate method |
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2015
- 2015-12-31 CN CN201511022906.4A patent/CN105418792A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6465101A (en) * | 1987-09-05 | 1989-03-10 | Takeshi Ishii | Water hyacinth pectin |
| CN101148482A (en) * | 2007-11-05 | 2008-03-26 | 桂林工学院 | Method for extracting pectin from banana peel |
| CN104031172A (en) * | 2014-06-03 | 2014-09-10 | 沈阳师范大学 | Method for extracting pectin from banana peel by ultrasonic synergistic ammonium oxalate method |
Non-Patent Citations (1)
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| 陶艳丽等: "超声波协同草酸铵法提取香蕉皮中果胶的研究", 《粮食与油脂》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108567143A (en) * | 2018-03-28 | 2018-09-25 | 广西玉阳绿州生物科技有限公司 | Lowiaceae plant extraction liquid, production method and its application in treatment of cancer |
| CN111500656A (en) * | 2020-04-14 | 2020-08-07 | 广西农业职业技术学院 | Method for extracting low-molecular-weight pectin from banana peel |
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Application publication date: 20160323 |