CN105418192A - Cordyceps militaris strain culture medium and preparation method thereof - Google Patents
Cordyceps militaris strain culture medium and preparation method thereof Download PDFInfo
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- CN105418192A CN105418192A CN201510889837.0A CN201510889837A CN105418192A CN 105418192 A CN105418192 A CN 105418192A CN 201510889837 A CN201510889837 A CN 201510889837A CN 105418192 A CN105418192 A CN 105418192A
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- cordyceps militaris
- culture medium
- preparation
- substratum
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The invention provides a cordyceps militaris strain culture medium. The culture medium is prepared from the following ingredients with concentration by taking water as a solvent: 15-25/L peptone, 3-5g/L yeast powder, 15-30g/L glucose, 1.2-1.5g/L potassium dihydrogen phosphate, 0.8-1.2g/L sodium chloride, 1-1.2g/L magnesium sulfate and 4-10g/L powdered milk. By using the culture medium as a strain culture medium for culturing cordyceps militaris, the cordyceps militaris production rate can be effectively increased, the cordyceps militaris yield can be increased, and the problems of strain degradation and the like can be solved.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of Cordyceps militaris spawn substratum and preparation method thereof.
Background technology
Cordyceps militaris (L.) Link. take silkworm chrysalis as the fungi of host, belong to together with Cordyceps sinensis is equal, chemical composition is basically identical, its antineoplastic component cordycepin content is higher than Cordyceps sinensis, and be the new resource food of Ministry of Health's approval, there is antitumor, antiviral, antibacterial, anti-inflammatory, delay senility, improve the multiple efficacies such as immunizing power, be suitable for crowd extensive.The artificial culture of Cordyceps militaris (L.) Link. and exploitation in recent years develops rapidly, however the bacterial classification of Cordyceps militaris (L.) Link. to there will be mycelial growth through a few culture slow, annesl is slow, and vitality declines, and goes out careless decline, goes out that grass is slow waits bacterial classification decay.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of Cordyceps militaris spawn substratum and preparation method thereof, cultivate Cordyceps militaris (L.) Link. using this substratum as bacterium culture medium and effectively can improve and carelessness, improving Cordyceps militaris (L.) Link. output, solving the problems such as spawn degeneration.
Cordyceps militaris spawn substratum provided by the invention take water as solvent, is made up of the composition of following concentration:
As screening formulation, above-mentioned Cordyceps militaris spawn substratum is made up of the composition of following concentration:
The present invention further provides the preparation method of above-mentioned Cordyceps militaris spawn substratum, specifically comprise the steps: peptone, yeast powder, glucose, KH with pure water
2pO
4, NaCl, MgSO
4, milk powder dissolves and fully mixing, is distributed into culturing bottle, sealing, sterilizing, cooling.
The actual conditions of described sterilizing is: 121 DEG C, 1.1Mpa sterilizing 20 ~ 45min.
Cordyceps militaris spawn culture medium prescription of the present invention is reasonable, and between each composition, synergy is good.Substratum of the present invention is used to cultivate Cordyceps militaris (L.) Link. again as after bacterium culture medium activation Cordyceps militaris spawn, comprehensive nutrition is balanced, effectively can change that mycelial growth is slow, annesl is slow, it is slow to go out grass, vigor is low, go out the phenomenons such as careless low, the Cordyceps sporophore stable yield obtained, can effectively reduce or slow down the degradation phenomena of Cordyceps militaris spawn.
Embodiment
Be further described in detail the present invention below in conjunction with embodiment, to make those skilled in the art the present invention may be better understood and can be implemented, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1:
One, the preparation of Cordyceps militaris spawn substratum:
The present embodiment Cordyceps militaris spawn substratum take water as solution, is made up of the composition of following concentration:
Be prepared as follows: take each composition by above formulation by weight, add pure water 1L, fully dissolve and be distributed into triangle culturing bottle, every bottled 200ml, 121 DEG C, 1.1Mpa sterilizing 30 ~ 40min after mixing, for subsequent use after its naturally cooling.
Two, the activation of Cordyceps militaris spawn
1, in super clean bench, use the bacterium culture medium of above-mentioned preparation to inoculate the Chinese caterpillar fungus thalline (bacterial classification is Cordycepsmilitaris (L) Link) of obviously degenerating, inoculative proportion is 1:100.
2, lucifuge quiescent culture 24h under temperature 22 DEG C of conditions, then continues under temperature 22 DEG C, 170rpm to cultivate 60-72h, makes bacterial concentration reach every milliliter of 3-5 bacterium ball.
Three, the cultivation of Cordyceps militaris (L.) Link.
Common method is adopted to carry out the sterile culture of Cordyceps militaris (L.) Link., results and drying.Specific as follows:
1, in super clean bench, inoculate the Cordyceps militaris (L.) Link. thalline of activation to solid medium, every bottle of 50ml solid medium inoculation 1mL activates bacterium liquid.Solid culture based formulas is as follows: dry dried silkworm chrysalis meal 30g/L, rice meal 170g/L, potassium primary phosphate 1.2g/L, SODIUM PHOSPHATE, MONOBASIC 0.8g/L, extractum carnis 1g/L.
2, under temperature 22 DEG C, humidity 75 ~ 85% condition, lucifuge is cultivated 3 ~ 5 days, makes Cordyceps mycelium cover with media surface rapidly, prevents other miscellaneous bacterias from growing in media surface.
3,5th ~ 20 days illumination cultivation, daytime 12 hours of daylight light photograph, temperature 20 ~ 22 DEG C (being preferably 22 DEG C), humidity 80 ~ 85%, intensity of illumination controls at 100 ~ 150Lx; 12 hours evenings black out, temperature 18 ~ 20 DEG C (best 18 DEG C), humidity 75 ~ 85%.Ventilate every day 2 times, sooner or later respectively once, time temperature is relatively low, carry out each 20 ~ 30 minutes.Evening on daytime, the temperature difference was conducive to being differentiated to form of former base.
4, h light on 21st ~ 35 day daytime 12 (natural light adds fluorescent lamp), temperature 20 ~ 22 DEG C (being preferably 22 DEG C), humidity 75% ~ 85%, intensity of illumination controls at 1000 ~ 1200Lx; 12 hours evenings black out, temperature 18 ~ 20 DEG C (being preferably 18 DEG C), humidity 75 ~ 85%.This time is sporophore growth period, the growth of high light to sporophore is favourable, makes it be that bar-shaped growth is not broken into ripe, healthy and strong, color and luster and becomes bright-coloured orange-yellow, ventilates 2 times every day at this growth phase, sooner or later respectively once, each 20 ~ 30 minutes are carried out time temperature is relatively low.
5, gather in the crops and cultivate again, to outward winding cultivation bottle cap, tweezers are with after 75% alcohol disinfecting, sporophore is taken out (only taking out the part above substratum), the sporophore of taking-up is placed in clean Stainless Steel Disc, select uniform color, sturdy, complete sporophore, remove remaining substratum be placed on weigh in new Stainless Steel Disc for subsequent use; In culturing bottle, remainder presses culture condition and the method continuation cultivation of 20th ~ 35 days, again gathers in the crops.
Precaution in cultivation:
Scattered light when A, illumination, and directing light can not be used, the growth of Chinese caterpillar fungus has phototropism, will carry out rolling bottle to the bottle in the place of uniform illumination, makes its uniform illumination, and sporophore upwards grows.
B, in culturing process, want strict temperature control and humidity, so strictly will control temperature well.When current workshop there is not control temperature equipment, control the temperature of culturing room with ground watering, watering in general a day 3 times, but also will become according to the temperature situation on the same day, temperature height will spill more, culturing room's temperature more than 25 DEG C, cooling of turning on the aircondition.
C, every day culturing room want ventilation one to twice, selection of time in the morning relative with dusk temperature low time, each 20 ~ 30 minutes.
The bottle of D, inoculation after stain will be taken out timely, and the substratum of the inside is poured out renewed vaccination after sterilizing again, the bottle polluted can not be allowed to be placed in culturing room and cultivate, and cause whole culturing room by the pollution of miscellaneous bacteria.
Embodiment 2:
One, the preparation of Cordyceps militaris spawn substratum:
The present embodiment Cordyceps militaris spawn substratum take water as solution, is made up of the composition of following concentration:
Be prepared as follows: take each composition by above formulation by weight, add pure water 1L, fully dissolve and be distributed into triangle culturing bottle, every bottled 200ml, 121 DEG C, 1.1Mpa sterilizing 30 ~ 40min after mixing, for subsequent use after its naturally cooling.
Activation and the cultivation of Cordyceps militaris (L.) Link. Cordycepsmilitaris (L) Link bacterial classification is carried out by the method that embodiment 1 is identical.
Embodiment 3:
One, the preparation of Cordyceps militaris spawn substratum:
The present embodiment Cordyceps militaris spawn substratum take water as solution, is made up of the composition of following concentration:
Be prepared as follows: take each composition by above formulation by weight, add pure water 1L, fully dissolve and be distributed into triangle culturing bottle, every bottled 200ml, 121 DEG C, 1.1Mpa sterilizing 30 ~ 40min after mixing, for subsequent use after its naturally cooling.
Activation and the cultivation of Cordyceps militaris (L.) Link. Cordycepsmilitaris (L) Link bacterial classification is carried out by the method that embodiment 1 is identical.
Embodiment 4:
One, the preparation of Cordyceps militaris spawn substratum:
The present embodiment Cordyceps militaris spawn substratum take water as solution, is made up of the composition of following concentration:
Be prepared as follows: take each composition by above formulation by weight, add pure water 1L, fully dissolve and be distributed into triangle culturing bottle, every bottled 200ml, 121 DEG C, 1.1Mpa sterilizing 30 ~ 40min after mixing, for subsequent use after its naturally cooling.
Activation and the cultivation of Cordyceps militaris (L.) Link. Cordycepsmilitaris (L) Link bacterial classification is carried out by the method that embodiment 1 is identical.
Embodiment 5:
One, the preparation of Cordyceps militaris spawn substratum:
The present embodiment Cordyceps militaris spawn substratum take water as solution, is made up of the composition of following concentration:
Be prepared as follows: take each composition by above formulation by weight, add pure water 1L, fully dissolve and be distributed into triangle culturing bottle, every bottled 200ml, 121 DEG C, 1.1Mpa sterilizing 30 ~ 40min after mixing, for subsequent use after its naturally cooling.
Activation and the cultivation of Cordyceps militaris (L.) Link. Cordycepsmilitaris (L) Link bacterial classification is carried out by the method that embodiment 1 is identical.
The Cordyceps militaris (L.) Link. dry product above embodiment obtained carries out calculating of weighing, and result is as shown in table 1 below.
Table 1: Cordyceps militaris (L.) Link. dry product weight
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Weight (g) | 1.07 | 0.98 | 1.03 | 1.01 | 0.94 |
As seen from the above embodiment, cultivate after using bacterium culture medium of the present invention to activate Cordyceps militaris (L.) Link. Cordycepsmilitaris (L) Link bacterial classification, the Cordyccps-militaris-(L.)-link. Sporophore Dried product obtained between 0.94 ~ 1.07g, and drops to about 0.8g according to the every bottle entity dry weight productive rate of normal cultivation by 1 original ~ 1.2g.So utilize substratum provided by the present invention to cultivate as after bacterium culture medium activation Cordyceps militaris spawn the degradation phenomena effectively can alleviating Cordyceps militaris spawn.
The above embodiment of the present invention can not be used for limiting the present invention to explanation of the present invention, and any change in the implication suitable with claims of the present invention and scope, all should think to be included in the scope of claims.
Claims (4)
1. a Cordyceps militaris spawn substratum, is characterized in that: described substratum take water as solvent, is made up of the composition of following concentration:
2. Cordyceps militaris spawn substratum according to claim 1, is characterized in that: be made up of the composition of following concentration:
3. the preparation method of Cordyceps militaris spawn substratum according to claim 1, is characterized in that step is as follows: with pure water by peptone, yeast powder, glucose, KH
2pO
4, NaCl, MgSO
4, milk powder dissolves and fully mixing, is distributed into culturing bottle, sealing, sterilizing, cooling.
4. the preparation method of Cordyceps militaris spawn substratum according to claim 3, is characterized in that: the condition of described sterilizing is: 121 DEG C, 1.1Mpa sterilizing 20 ~ 45min.
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| CN201510889837.0A CN105418192A (en) | 2015-12-04 | 2015-12-04 | Cordyceps militaris strain culture medium and preparation method thereof |
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| CN201510889837.0A CN105418192A (en) | 2015-12-04 | 2015-12-04 | Cordyceps militaris strain culture medium and preparation method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772876A (en) * | 2005-09-30 | 2006-05-17 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN103981105A (en) * | 2014-05-29 | 2014-08-13 | 熊艳 | Method for cultivating cordyceps militaris with high yield of cordycepic acid |
| CN103988712A (en) * | 2014-05-29 | 2014-08-20 | 熊艳 | High-yield polysaccharide cordyceps militaris cultivation method |
| CN104164367A (en) * | 2014-08-01 | 2014-11-26 | 沁阳市西向食用菌研究所 | Dried silkworm cordyceps militaris and culture method thereof |
-
2015
- 2015-12-04 CN CN201510889837.0A patent/CN105418192A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772876A (en) * | 2005-09-30 | 2006-05-17 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN103981105A (en) * | 2014-05-29 | 2014-08-13 | 熊艳 | Method for cultivating cordyceps militaris with high yield of cordycepic acid |
| CN103988712A (en) * | 2014-05-29 | 2014-08-20 | 熊艳 | High-yield polysaccharide cordyceps militaris cultivation method |
| CN104164367A (en) * | 2014-08-01 | 2014-11-26 | 沁阳市西向食用菌研究所 | Dried silkworm cordyceps militaris and culture method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王贺祥，刘庆洪: "《食用菌栽培学 第2版》", 31 March 2014, 中国农业大学出版社 * |
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Application publication date: 20160323 |