CN105417690A - Culture method of aerobiotic active sludge - Google Patents
Culture method of aerobiotic active sludge Download PDFInfo
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- CN105417690A CN105417690A CN201510791513.3A CN201510791513A CN105417690A CN 105417690 A CN105417690 A CN 105417690A CN 201510791513 A CN201510791513 A CN 201510791513A CN 105417690 A CN105417690 A CN 105417690A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/02—Non-contaminated water, e.g. for industrial water supply
- C02F2103/026—Treating water for medical or cosmetic purposes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a culture method of aerobiotic active sludge, and belongs to the technical field of sludge culture. The method includes the steps that firstly, fresh moss is introduced into a septic tank, and anaerobic fermentation is performed till the moss turns yellow and black; secondly, cultured biological bacteria are introduced into the septic tank after fermentation, so that the surface of the fermented matter is covered with a layer of white hyphae and is in a slurry state; thirdly, kieselguhr is introduced into wastewater, stuffy exposure treatment is performed, then the prepared fermented matter in the slurry state is put into the wastewater, stuffy exposure treatment is performed again, and the faint yellow active sludge can be obtained. As proved by the embodiment, the formation time of the aerobiotic granular sludge is shortened by 80-85%; besides, the prepared sludge is high in granulation degree, a large amount of flocculent sludge is avoided, and thus the capacity in the wastewater treatment process is significantly improved.
Description
Technical field
The invention discloses a kind of cultural method of aerobic activated sludge, belong to culturing sludge technical field.
Background technology
Since 11th Five-Year, country increases the dynamics for environmental pollution improvement, especially in water environmental management, has carried out national science and technology key special subjects-water pollution Control and treatment, it drops into up to 112.66 hundred million yuan, and this numeral exceedes 14,000,000,000 yuan by increasing to further during 12.This measure is the body eutrophication problem that the nitrogen phosphoric in order to solve excess emissions in recent years causes.According to ambient condition publication display in 2012, national surface water was totally slight pollution, and lake eutrophication situation is serious, and nitrogen phosphorus is still principal pollutant.Whole nation ammonia nitrogen total emission volumn is 2,500,000 tons, and wherein the quantity discharged in industrial source and life source reaches 1,710,000 tons, 67% of ammonia nitrogen total emission volumn of standing, and therefore, processes point-source pollutions such as industry lives, is the effective way alleviating water body environment crisis.
Active sludge is the general name of microbial population and their organic substances of depending on and inorganic substance, be mainly used to process stain disease, be made up of organic in the sewage of the microbial populations such as bacterium, zoogloea, protozoon, metazoan and absorption and inorganic substance, there is certain vigor, there is the velvet-like mud of wadding of good speech sewage function.
According to the difference of electron acceptor(EA) in metabolic process, active sludge can be divided into anaerobic grain sludge and aerobic particle mud again.Aerobic granular is a kind of special shape of biofilm development; it is cell self immobilization particle of microorganism spontaneous formation under aerobic condition; compared with anaerobic grain sludge; aerobic particle mud has unique advantage, and such as process range is wide, high adsorption capacity; but also there are some shortcomings in aerobic particle mud; such as aerobic particle mud not easily formed and formation time long, the nearly sky up to a hundred had, these shortcomings seriously limit aerobic particle mud application in practice.
Aerobic cultivation conventional at present has activated sludge process, continuously cultivation method and dewatered sludge inoculation cultivation method.Wherein activated sludge process points in waste water to pass into air continuously, the sludge-like object formed because of aerobic microbial reproduction after certain hour, but the method exists a large amount of floc sludge; Continuous cultivation method in addition first full waste water is entered in aeration tank, then stops water inlet, can continuum micromeehanics after vexed half a day to one day of exposing to the sun, but the obtained granular sludge degree of the method is not high, and sludge loading is not high; Last dewatered sludge inoculation cultivation method be by mud cake after water extracter dewaters, add in aeration tank, and it needs before adding to add a small amount of water and puddling, but this method may containing certain chemical agent, toxicity ratio is comparatively large, and processing power is poor.
Summary of the invention
The technical problem that the present invention mainly solves: not easily formed for current aerobic particle mud, formation time is long; and in the process of the aerobic cultivation of tradition; exist granular sludge degree not high, containing a large amount of floc sludge; and the shortcoming that processing power is poor, provide a kind of cultural method of aerobic activated sludge.First fresh liver moss is introduced in septic tank by the method, carry out anaerobically fermenting to blackening of turning to be yellow, introduce in the septic tank after fermentation by cultivating the biological bacteria obtained again afterwards, make fermented product surface coverage one deck white hypha and in sposh state, next introduce diatomite by waste water again, after carrying out vexed process of exposing to the sun, more obtained sposh shape fermented product is put into waste water, again carry out vexed process of exposing to the sun, faint yellow active sludge can be obtained.The present invention not only makes the formation time of aerobic particle mud obtain shortening, and the granular sludge degree made is high, does not have a large amount of floc sludges, and in process waste water process, ability obtains significant raising.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
(1) in septic tank, drop into the fresh liver moss that pond holds 10 ~ 15%, after stirring, sealing septic tank, carry out anaerobically fermenting process, control temperature is 30 ~ 50 DEG C, ferments 8 ~ 15 days, makes liver moss jaundice blackening in septic tank;
(2) count by weight, choose that 5 ~ 15 parts of Trypsins are old, the yeast of 20 ~ 40 parts of agar, 20 ~ 65 parts of inositols and 15 ~ 20 parts, be uniformly mixed rear formation substratum, add 200 ~ 300mL deionized water afterwards and regulate pH to be 6.0 ~ 6.3;
(3) after complete pH to be regulated, be warming up to 130 ~ 150 DEG C and carry out sterilising treatment, obtained microbiological culture media after 20 ~ 30min;
(4) by containing aerobic denitrifying bacteria, the mud taking the photograph phosphorus bacterium, thread fungus and nitrifier, be seeded in mentioned microorganism substratum by inoculum size than 1: 1: 1: 1, temperature is regulated to be 18 ~ 28 DEG C, quiescent culture 10 ~ 15h, after cultivation completes, be placed in whizzer, with the rotating speed centrifugation 15 ~ 20min of 1000 ~ 1500r/min, remove supernatant liquid, stay mixed strains mud;
(5) mixed strains mud obtained above is put into the septic tank of above-mentioned steps (1) after sealing and fermenting, stir, again carry out anaerobically fermenting process, control temperature is 35 ~ 45 DEG C, when fermented product surface coverage one deck white hypha, stop fermentation, be warming up to 40 ~ 55 DEG C with the speed program of 0.5 DEG C/min more afterwards, when making fermented product be sposh state, open sealed fermenting pond, pass into aeration flexible pipe simultaneously, carry out aeration, until dissolved oxygen reaches 1.5 ~ 2.0mg/L in pond;
(6) in an aeration tank, introduce pharmaceutical chemical industry waste water, add diatomite, uniform stirring simultaneously, sludge content in waste water is made to be 3000 ~ 4000mg/L, then pass into air aeration, after aeration 5 ~ 8h, stop aeration, airtight standing 1 ~ 2h, add the fermented product in the above-mentioned steps (5) of 60 ~ 80% of diatomite quality afterwards, stir, again pass into air aeration, after aeration 10 ~ 12h, stop aeration, airtight standing 2 ~ 3h, can obtain faint yellow active sludge.
Application method of the present invention is: put into by the aerobic activated sludge obtained by the present invention in pending pharmaceutical chemical industry waste water, wherein add 5 ~ 8kg aerobic activated sludge in every 1 ~ 3t trade effluent, leave standstill 15 ~ 18 days afterwards, pharmaceutical chemical industry waste water content is detected with detector, obviously can record COD content in pharmaceutical chemical industry waste water and be reduced to 200 ~ 250mg/L from 4000 ~ 8000mg/L, clearance is more than 90%.
The invention has the beneficial effects as follows:
(1) the present invention's method in preparation process is simple, makes the formation time of aerobic particle mud shorten 80 ~ 85%;
(2) the granular sludge degree not only made of the present invention is high, up to 3500 ~ 4000mg/L, and does not have a large amount of floc sludges, makes ability in process waste water process obtain significant raising.
Embodiment
First in septic tank, drop into the fresh liver moss that pond holds 10 ~ 15%, after stirring, sealing septic tank, carry out anaerobically fermenting process, control temperature is 30 ~ 50 DEG C, ferments 8 ~ 15 days, makes liver moss jaundice blackening in septic tank; Then count by weight, choose that 5 ~ 15 parts of Trypsins are old, the yeast of 20 ~ 40 parts of agar, 20 ~ 65 parts of inositols and 15 ~ 20 parts, be uniformly mixed rear formation substratum, add 200 ~ 300mL deionized water afterwards and regulate pH to be 6.0 ~ 6.3; Afterwards after complete pH to be regulated, be warming up to 130 ~ 150 DEG C and carry out sterilising treatment, obtained microbiological culture media after 20 ~ 30min; Next by containing aerobic denitrifying bacteria, the mud taking the photograph phosphorus bacterium, thread fungus and nitrifier, be seeded in mentioned microorganism substratum by inoculum size than 1: 1: 1: 1, temperature is regulated to be 18 ~ 28 DEG C, quiescent culture 10 ~ 15h, after cultivation completes, be placed in whizzer, with the rotating speed centrifugation 15 ~ 20min of 1000 ~ 1500r/min, remove supernatant liquid, stay mixed strains mud;
Again mixed strains mud obtained above is put into the septic tank of above-mentioned steps after sealing and fermenting, stir, again carry out anaerobically fermenting process, control temperature is 35 ~ 45 DEG C, when fermented product surface coverage one deck white hypha, stop fermentation, be warming up to 40 ~ 55 DEG C with the speed program of 0.5 DEG C/min more afterwards, when making fermented product be sposh state, open sealed fermenting pond, pass into aeration flexible pipe simultaneously, carry out aeration, until dissolved oxygen reaches 1.5 ~ 2.0mg/L in pond; Introduce pharmaceutical chemical industry waste water in the most backward aeration tank, add diatomite simultaneously, uniform stirring, sludge content in waste water is made to be 3000 ~ 4000mg/L, then pass into air aeration, after aeration 5 ~ 8h, stop aeration, airtight standing 1 ~ 2h, add the fermented product in the above-mentioned steps of 60 ~ 80% of diatomite quality afterwards, stir, again pass into air aeration, after aeration 10 ~ 12h, stop aeration, airtight standing 2 ~ 3h, can obtain faint yellow active sludge.
Example 1
First in septic tank, drop into the fresh liver moss that pond holds 10%, after stirring, sealing septic tank, carry out anaerobically fermenting process, control temperature is 30 DEG C, ferments 8 days, makes liver moss jaundice blackening in septic tank; Then count by weight, choose that 5 parts of Trypsins are old, the yeast of 20 parts of agar, 20 parts of inositols and 15 parts, be uniformly mixed rear formation substratum, add 200mL deionized water afterwards and regulate pH to be 6.0; Afterwards after complete pH to be regulated, be warming up to 130 DEG C and carry out sterilising treatment, obtained microbiological culture media after 20min; Next by containing aerobic denitrifying bacteria, the mud taking the photograph phosphorus bacterium, thread fungus and nitrifier, be seeded in mentioned microorganism substratum by inoculum size than 1: 1: 1: 1, temperature is regulated to be 18 DEG C, quiescent culture 10h, after cultivation completes, be placed in whizzer, with the rotating speed centrifugation 15min of 1000r/min, remove supernatant liquid, stay mixed strains mud; Again mixed strains mud obtained above is put into the septic tank of above-mentioned steps after sealing and fermenting, stir, again carry out anaerobically fermenting process, control temperature is 35 DEG C, when fermented product surface coverage one deck white hypha, stop fermentation, be warming up to 40 DEG C with the speed program of 0.5 DEG C/min more afterwards, when making fermented product be sposh state, open sealed fermenting pond, pass into aeration flexible pipe simultaneously, carry out aeration, until dissolved oxygen reaches 1.5mg/L in pond; Introduce pharmaceutical chemical industry waste water in the most backward aeration tank, add diatomite simultaneously, uniform stirring, sludge content in waste water is made to be 3000mg/L, then pass into air aeration, after aeration 5h, stop aeration, airtight standing 1h, add the fermented product in the above-mentioned steps of 60% of diatomite quality afterwards, stir, again pass into air aeration, after aeration 10h, stop aeration, airtight standing 2h, can obtain faint yellow active sludge.
This example operation is simple, aerobic activated sludge obtained by the present invention is put in pending pharmaceutical chemical industry waste water, wherein add 5kg aerobic activated sludge in every 1t trade effluent, leave standstill 15 days afterwards, pharmaceutical chemical industry waste water content is detected with detector, obviously can record COD content in pharmaceutical chemical industry waste water and be reduced to 200mg/L from 4000mg/L, clearance is 95%.
Example 2
First in septic tank, drop into the fresh liver moss that pond holds 13%, after stirring, sealing septic tank, carry out anaerobically fermenting process, control temperature is 40 DEG C, ferments 12 days, makes liver moss jaundice blackening in septic tank; Then count by weight, choose that 10 parts of Trypsins are old, the yeast of 30 parts of agar, 45 parts of inositols and 18 parts, be uniformly mixed rear formation substratum, add 250mL deionized water afterwards and regulate pH to be 6.2; Afterwards after complete pH to be regulated, be warming up to 140 DEG C and carry out sterilising treatment, obtained microbiological culture media after 25min; Next by containing aerobic denitrifying bacteria, the mud taking the photograph phosphorus bacterium, thread fungus and nitrifier, be seeded in mentioned microorganism substratum by inoculum size than 1: 1: 1: 1, temperature is regulated to be 25 DEG C, quiescent culture 13h, after cultivation completes, be placed in whizzer, with the rotating speed centrifugation 18min of 1200r/min, remove supernatant liquid, stay mixed strains mud; Again mixed strains mud obtained above is put into the septic tank of above-mentioned steps after sealing and fermenting, stir, again carry out anaerobically fermenting process, control temperature is 40 DEG C, when fermented product surface coverage one deck white hypha, stop fermentation, be warming up to 45 DEG C with the speed program of 0.5 DEG C/min more afterwards, when making fermented product be sposh state, open sealed fermenting pond, pass into aeration flexible pipe simultaneously, carry out aeration, until dissolved oxygen reaches 1.8mg/L in pond; Introduce pharmaceutical chemical industry waste water in the most backward aeration tank, add diatomite simultaneously, uniform stirring, sludge content in waste water is made to be 3500mg/L, then pass into air aeration, after aeration 7h, stop aeration, airtight standing 1.5h, add the fermented product in the above-mentioned steps of 70% of diatomite quality afterwards, stir, again pass into air aeration, after aeration 11h, stop aeration, airtight standing 2.5h, can obtain faint yellow active sludge.
This example operation is simple, aerobic activated sludge obtained by the present invention is put in pending pharmaceutical chemical industry waste water, wherein add 7kg aerobic activated sludge in every 2t trade effluent, leave standstill 16 days afterwards, pharmaceutical chemical industry waste water content is detected with detector, obviously can record COD content in pharmaceutical chemical industry waste water and be reduced to 230mg/L from 6500mg/L, clearance is 96.5%.
Example 3
First in septic tank, drop into the fresh liver moss that pond holds 15%, after stirring, sealing septic tank, carry out anaerobically fermenting process, control temperature is 50 DEG C, ferments 15 days, makes liver moss jaundice blackening in septic tank; Then count by weight, choose that 15 parts of Trypsins are old, the yeast of 40 parts of agar, 65 parts of inositols and 20 parts, be uniformly mixed rear formation substratum, add 300mL deionized water afterwards and regulate pH to be 6.3; Afterwards after complete pH to be regulated, be warming up to 150 DEG C and carry out sterilising treatment, obtained microbiological culture media after 30min; Next by containing aerobic denitrifying bacteria, the mud taking the photograph phosphorus bacterium, thread fungus and nitrifier, be seeded in mentioned microorganism substratum by inoculum size than 1: 1: 1: 1, temperature is regulated to be 28 DEG C, quiescent culture 15h, after cultivation completes, be placed in whizzer, with the rotating speed centrifugation 20min of 1500r/min, remove supernatant liquid, stay mixed strains mud; Again mixed strains mud obtained above is put into the septic tank of above-mentioned steps after sealing and fermenting, stir, again carry out anaerobically fermenting process, control temperature is 45 DEG C, when fermented product surface coverage one deck white hypha, stop fermentation, be warming up to 55 DEG C with the speed program of 0.5 DEG C/min more afterwards, when making fermented product be sposh state, open sealed fermenting pond, pass into aeration flexible pipe simultaneously, carry out aeration, until dissolved oxygen reaches 2.0mg/L in pond; Introduce pharmaceutical chemical industry waste water in the most backward aeration tank, add diatomite simultaneously, uniform stirring, sludge content in waste water is made to be 4000mg/L, then pass into air aeration, after aeration 8h, stop aeration, airtight standing 2h, add the fermented product in the above-mentioned steps of 80% of diatomite quality afterwards, stir, again pass into air aeration, after aeration 12h, stop aeration, airtight standing 3h, can obtain faint yellow active sludge.
This example operation is simple, aerobic activated sludge obtained by the present invention is put in pending pharmaceutical chemical industry waste water, wherein add 8kg aerobic activated sludge in every 3t trade effluent, leave standstill 18 days afterwards, pharmaceutical chemical industry waste water content is detected with detector, obviously can record COD content in pharmaceutical chemical industry waste water and be reduced to 250mg/L from 8000mg/L, clearance is 96.8%.
Claims (1)
1. a cultural method for aerobic activated sludge, is characterized in that concrete operation step is:
(1) in septic tank, drop into the fresh liver moss that pond holds 10 ~ 15%, after stirring, sealing septic tank, carry out anaerobically fermenting process, control temperature is 30 ~ 50 DEG C, ferments 8 ~ 15 days, makes liver moss jaundice blackening in septic tank;
(2) count by weight, choose that 5 ~ 15 parts of Trypsins are old, the yeast of 20 ~ 40 parts of agar, 20 ~ 65 parts of inositols and 15 ~ 20 parts, be uniformly mixed rear formation substratum, add 200 ~ 300mL deionized water afterwards and regulate pH to be 6.0 ~ 6.3;
(3) after complete pH to be regulated, be warming up to 130 ~ 150 DEG C and carry out sterilising treatment, obtained microbiological culture media after 20 ~ 30min;
(4) by containing aerobic denitrifying bacteria, the mud taking the photograph phosphorus bacterium, thread fungus and nitrifier, be seeded in mentioned microorganism substratum by inoculum size than 1: 1: 1: 1, temperature is regulated to be 18 ~ 28 DEG C, quiescent culture 10 ~ 15h, after cultivation completes, be placed in whizzer, with the rotating speed centrifugation 15 ~ 20min of 1000 ~ 1500r/min, remove supernatant liquid, stay mixed strains mud;
(5) mixed strains mud obtained above is put into the septic tank of above-mentioned steps (1) after sealing and fermenting, stir, again carry out anaerobically fermenting process, control temperature is 35 ~ 45 DEG C, when fermented product surface coverage one deck white hypha, stop fermentation, be warming up to 40 ~ 55 DEG C with the speed program of 0.5 DEG C/min more afterwards, when making fermented product be sposh state, open sealed fermenting pond, pass into aeration flexible pipe simultaneously, carry out aeration, until dissolved oxygen reaches 1.5 ~ 2.0mg/L in pond;
(6) in an aeration tank, introduce pharmaceutical chemical industry waste water, add diatomite, uniform stirring simultaneously, sludge content in waste water is made to be 3000 ~ 4000mg/L, then pass into air aeration, after aeration 5 ~ 8h, stop aeration, airtight standing 1 ~ 2h, add the fermented product in the above-mentioned steps (5) of 60 ~ 80% of diatomite quality afterwards, stir, again pass into air aeration, after aeration 10 ~ 12h, stop aeration, airtight standing 2 ~ 3h, can obtain faint yellow active sludge.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106865750A (en) * | 2017-01-18 | 2017-06-20 | 常州大学 | A kind of activated sludge culture and acclimation method for difficult for biological degradation organic wastewater biological treatment |
| CN110016447A (en) * | 2019-04-22 | 2019-07-16 | 刘坚 | A method of strain is extracted using pig manure |
| CN115959766A (en) * | 2023-02-07 | 2023-04-14 | 广州众行环保科技有限公司 | Method for culturing activated sludge in multiple times |
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| CN106865750A (en) * | 2017-01-18 | 2017-06-20 | 常州大学 | A kind of activated sludge culture and acclimation method for difficult for biological degradation organic wastewater biological treatment |
| CN110016447A (en) * | 2019-04-22 | 2019-07-16 | 刘坚 | A method of strain is extracted using pig manure |
| CN115959766A (en) * | 2023-02-07 | 2023-04-14 | 广州众行环保科技有限公司 | Method for culturing activated sludge in multiple times |
| CN115959766B (en) * | 2023-02-07 | 2024-03-19 | 广州众行环保科技有限公司 | Fractional culture method of activated sludge |
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Inventor after: Yin Hang Inventor after: Yang Zhidan Inventor after: He Longbin Inventor after: Duan Konglin Inventor after: Deng Qingwen Inventor after: Yan Yan Inventor before: Chou Yingchao Inventor before: Zhang Fan |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20171120 Address after: Chunhui road 400010 Chongqing Dadukou Cypress Street Road No. 101 building 3 6-4 Applicant after: Chongqing Qingxi Environmental Protection Technology Co., Ltd. Address before: 100086 Beijing city Haidian District North Sanhuan Road 43, Tsing Wun contemporary building 12A11 Applicant before: BEIJING ZHITAO SCIENCE & TECHNOLOGY CO., LTD. |
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