CN105400687B - Human body extraction device of fallen cells under a kind of mirror - Google Patents
Human body extraction device of fallen cells under a kind of mirror Download PDFInfo
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- CN105400687B CN105400687B CN201511026942.8A CN201511026942A CN105400687B CN 105400687 B CN105400687 B CN 105400687B CN 201511026942 A CN201511026942 A CN 201511026942A CN 105400687 B CN105400687 B CN 105400687B
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- human body
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to human body extraction device of fallen cells under a kind of mirror, the device includes microscope, universal displacement connector and extraction probe, the extraction probe includes needle handle and syringe needle, organic adhesive is attached with the syringe needle, one end of the needle handle is rotatably connected to rotating shaft, the one end of the rotating shaft away from the needle handle is fixedly connected with the syringe needle, one end of the universal displacement connector is fixedly connected on the microscopical support, the other end is fixedly installed connector, the needle handle is removably connected with the connector, the extraction probe can be extended between the microscopical object lens and objective table with the displacement of the universal displacement connector, the fixing device for fixing object is provided with the microscopical objective table.Beneficial effect is simple in construction, practicality, with strong points, ageing good, saves fund, can both improve D. Lab's NA recall rates, facilitates basic unit legal medical expert to operate again.
Description
Technical field
The present invention relates to a kind of grass-roots method medicinal detection device, human body extraction device of fallen cells under specifically a kind of mirror.
Background technology
One of main contents that country grass-roots method medical professionals makees at present be in the scene of extracting it is any may adhesion be suspected of being guilty
The object of people, including：Instrument, clothing, gloves, contactant, bloodstain, salivary stain, seminal stain etc., these referred to as material evidences are right
Four kinds are summed up in the processing method of these material evidences, both vacuum suctions, adhesive tape adhesion, cotton swab, original clip.For
Different physical surface characteristics, use methods different above to shift human body cast-off cells with relatively effective, but also all
The problem of having different.On the whole, these four methods are all the method processing microscopic species with macroscopic view, with certain blindness
Property.First, the frequency of use of vacuum suction is not high, does not possess the unit of experimental facilities in basic unit, and this side will not be used substantially
Method carries out just extracting superior censorship again, and problem is, first, this method is harsher to operating environment, wants to accomplish not
Pollute sample, it is necessary to keep the environment for extracting transfer sample quite clean, but this point is done and there are many difficulties, second,
The daily DNA samples up to tens to be processed in laboratory examples up to a hundred, the more aobvious applicability of cumbersome method is not enough, just current
It is not that the major and serious criminal case part of sufficient manpower, material resources and fund can be provided mostly, but all occurring daily for DNA effect cases
The case usually such as theft, robbery, reply quantity it is huge, efficiency seems more crucial on the contrary；Secondly, adhesive tape adhesion, cotton swab
Wipe, original clip these three methods will in two kinds of situation for, if when adhering to obvious human body component on sample, such as：
Bloodstain and salivary stain, these three methods are fairly simple effectively, if on the contrary, do not extract target significantly on instrument, clothes, that
These methods are more followed blindly, and need to carry out making up omission using the method repeatedly extracted, and so result in poor in timeliness, wave
Take the situation of fund.Reflect in real work, the DNA recall rate wretched insufficiencies of usual case, it is impossible to service case well
Detection, the method cost performance for being said differently alternatively these examination of material evidences is very low.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of simple in construction, practicality, with strong points, ageing good, section
About fund, can both improve D. Lab's NA recall rates, facilitate human body cast-off cells under the mirror of basic unit legal medical expert operation to extract dress again
Put.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：Human body extraction device of fallen cells under a kind of mirror, bag
Include microscope, universal displacement connector and extract probe, the extraction probe includes being attached with needle handle and syringe needle, the syringe needle
Organic adhesive, one end of the needle handle is rotatably connected to rotating shaft, the one end of the rotating shaft away from the needle handle and the syringe needle
It is fixedly connected, one end of the universal displacement connector is fixedly connected on the microscopical support, and the other end is fixedly installed
There is connector, the needle handle is removably connected with the connector, the extraction probe can be with the universal displacement connector
Displacement extend between the microscopical object lens and objective table, be provided with the microscopical objective table for fixing object
Fixing device.
The beneficial effects of the invention are as follows：Object to be tested is fixed on the microscopical load by the fixing device
On thing platform, object to be tested is observed by the microscopical eyepiece, the human body adhered on object to be tested is found and comes off
After cell, the syringe needle of the probe is moved to human body cast-off cells on object to be tested using the universal displacement connector
Position, slowly promote the probe, make its syringe needle rolled along along object to be tested pick human body cast-off cells it is some after, move
Drive the probe and the probe is pulled down into censorship from the connector, the probe is tiny as disposable because of its
Articles for use are produced in batches, and simple in construction, practicality is with strong points, ageing good, save fund, can both improve D. Lab NA detections
Rate, facilitates human body extraction device of fallen cells under the mirror of basic unit legal medical expert operation again, and bonding human body using organic adhesive comes off carefully
Born of the same parents do not destroy its structure, are easy to smoothly detect the affiliated people of the human body cast-off cells, improve recall rate.
On the basis of above-mentioned technical proposal, the present invention can also do following improvement.
Further, the connector is the connecting rod of Band clamp, and the needle handle is clamped by the clip of the connecting rod, is realized described
Connector is removably connected with the needle handle, and reliability is high.
Further, the clip of the correspondence connecting rod offers clip slot on the needle handle, and the clip of the connecting rod sandwiches described
Clip slot is to clamp the needle handle, and the clip for improving the connecting rod clamps the reliability of the needle handle.
Further, the syringe needle is made up of glass fibre, and circumferentially offers thread groove, the syringe needle along its length
Good insulating, heat resistance is strong, corrosion resistance is good, high mechanical strength can adapt to different working environments, while thread groove expands
The big contact area of the syringe needle, it is viscous to take human body cast-off cells more stable, it is difficult for drop-off.
Further, the rotating shaft is breakable rotating shaft, and the rotating shaft can fracture, and reduces chemical examination volume, beneficial to censorship.
Further, the organic adhesive is epoxide-resin glue, bonds human body cast-off cells reliability high.
Further, the universal displacement connector is Universal cantilever, realizes the universal displacement of the universal displacement connector
While, structural stability is good.
Further, the fixing device includes pressure ring, two pieces of risers and air bag, and the microscopical objective table level is set
Put, the pressure ring is removably horizontally set on the microscopical objective table upper surface；Two pieces of risers distinguish vertical phase
To be fixedly installed on the microscopical objective table upper surface, the pressure ring both sides, through two pieces of risers difference screw threads
Horizontally disposed clamping screw is connected with, positive and negative twist of two clamping screws can mutually draw close or remote, two pressures
One end that tight screw rod is mutually drawn close is fixedly installed three pin holders respectively；The air bag is fixedly installed on the microscopical load
Soft matter (such as clothes), can be cut into the block with the microscopical similar size of objective table area by thing platform upper surface,
The extraction that the block carries out human body cast-off cells thereon is compressed using the pressure ring, also can be by adamant as described microscopical
On objective table, between two pieces of risers, twisting two clamping screws makes both mutually draw close, and is clamped using three pin
Part clamps the adamant, carries out the extraction of the human body cast-off cells on adamant, the air bag can also be filled in the bottle being crushed
Shape thing, the bottle shaped article distending that is crushed is made to the airbag aeration and is fixed on the microscopical objective table, progress is pressed
The extraction of human body cast-off cells on flat bottle shaped article, adapts to different objects to be checked, and easy to operate, practical value is high.
Further, the pressure ring is rectangle pressure ring, and can by many screws and the microscopical objective table upper surface
The connection of dismounting, realizes that the pressure ring is removably connected with the microscopical objective table upper surface, and it is high to compress reliability.
Brief description of the drawings
Fig. 1 is structural representation of the invention；
Fig. 2 is the structural representation of probe of the present invention；
Fig. 3 is the top view of microscopical objective table of the present invention；
In accompanying drawing, the list of parts representated by each label is as follows：
1st, microscope, 2, universal displacement connector, 3, connector, 4, probe, 5, pressure ring, 6, three pin holders, 7, riser,
8th, clamping screw, 9, clip slot, 10, needle handle, 11, rotating shaft, 12, syringe needle, 13, thread groove, 14, screw, 15, air bag.
Embodiment
The principle and feature of the present invention are described below in conjunction with accompanying drawing, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
As depicted in figs. 1 and 2, human body extraction device of fallen cells under a kind of mirror, including microscope 1, universal displacement connection
Part 2 and extraction probe 4, extracting probe 4 includes being attached with organic adhesive on needle handle 10 and syringe needle 12, syringe needle 12, needle handle 10
One end is rotatably connected to rotating shaft 11, and the one end of rotating shaft 11 away from needle handle 10 is fixedly connected with syringe needle 12, universal displacement connector 2
One end is fixedly connected on the support of microscope 1, and the other end is fixedly installed connector 3, and needle handle 10 and connector 3 are dismountable
Connection, extracting probe 4 can extend with the displacement of universal displacement connector 2 between the object lens and objective table of microscope 1；Microscope 1
Objective table on be provided with fixing device for fixing object.
As depicted in figs. 1 and 2, connector 3 is the connecting rod of Band clamp, and needle handle 10 is clamped by the clip of connecting rod, on needle handle 10
The clip of respective link offers clip slot 9, and the clip of connecting rod sandwiches clip slot 9 to clamp needle handle 10；Syringe needle 12 is by glass fibre system
Into, and thread groove 13 is circumferentially offered along its length, rotating shaft 11 is breakable rotating shaft, and organic adhesive is epoxy resin
Glue, universal displacement connector 2 is Universal cantilever.
As shown in figures 1 and 3, fixing device includes pressure ring 5, two pieces of risers 7 and air bag 15, the objective table water of microscope 1
Flat to set, pressure ring 5 is removably horizontally set on the objective table upper surface of microscope 1, and pressure ring 5 passes through positioned at 5 four jiaos of pressure ring
Four screws 14 are removably connected with the objective table upper surface of microscope 1；Two pieces of risers 7 are relatively fixed are arranged on respectively vertically
Objective table upper surface, the both sides of pressure ring 5 of microscope 1, horizontally disposed compression spiral shell is threaded with through two pieces of risers 7 respectively
Bar 8, positive and negative twist of two clamping screws 8 can mutually be drawn close or remote, and one end that two clamping screws 8 are mutually drawn close is fixed respectively
It is provided with three pin holders 6；Air bag 15 is fixedly installed on the objective table upper surface of microscope 1；Pressure ring 5 is rectangle pressure ring, and is led to
Four screws 14 crossed positioned at 5 four jiaos of pressure ring are removably connected with the objective table upper surface of microscope 1.
Extracting method：
1st, the human body cast-off cells on general solid tool, the screwdriver of such as theft are extracted.First, screwdriver is put
On the objective table of microscope 1, between two pieces of risers 7, twisting two clamping screws 8 makes the two mutually draw close, and then makes correspondence
Three pin holders 6 screwdriver is fixedly clamped；Secondly, adjustment microscope 1, finds target body on screwdriver surface and comes off carefully
Born of the same parents；Then, promote Universal cantilever that the syringe needle 12 of probe 4 is moved to the position of the target body cast-off cells that correspondence is sought on screwdriver
Put, glued using the epoxide-resin glue adhered on syringe needle 12 on screwdriver surface and take target body cast-off cells；Finally, probe is removed
4, encapsulation preserves censorship, and DNA inspections can directly be carried out by having ready conditions, and for convenience of examining during inspection, rotating shaft 11 can fracture, take syringe needle
Test 12 parts.
2nd, the human body cast-off cells on bottle shaped article are extracted, such as suspect pinches flat beverage bottle.First, beverage bottle is placed on aobvious
On the objective table of micro mirror 1, between two pieces of risers 7, air bag 15 is filled in beverage bottle, inflated into air bag 15 beverage bottle is swollen
Open, twisting two clamping screws 8 makes the two mutually draw close, and then corresponding three pin holder 6 is fastened beverage bottle clamp；Its
Secondary, adjustment microscope 1 finds target body cast-off cells in beverage bottle surface；Then, Universal cantilever is promoted by the pin of probe 4
First 12 move to the position of the target body cast-off cells that correspondence is sought on beverage bottle, utilize the epoxide-resin glue adhered on syringe needle 12
Glued in beverage bottle surface and take target body cast-off cells；Finally, probe 4 is removed, encapsulation preserves censorship, and having ready conditions directly to be carried out
DNA is examined, and for convenience of examining during inspection, rotating shaft 11 can be fractureed, be taken the part of syringe needle 12 to test.
3rd, the human body cast-off cells of the soft form objects such as fabric, the clothing fragment of such as suspect are extracted.First, by clothing
Fragment is placed on the objective table of microscope 1, clothing fragment is excessive between two pieces of risers 7, can suitably cut out, pressure ring 5 is pressed in into clothing
On thing fragment, the corresponding fixing clothes fragment of screw 14 is tightened；Secondly, adjustment microscope 1, target is found in clothing fractal surfaces
Human body cast-off cells；Then, promote Universal cantilever that the syringe needle 12 of probe 4 is moved to the target body that correspondence is sought on clothing fragment
The position of cast-off cells, is glued in clothing fractal surfaces using the epoxide-resin glue adhered on syringe needle 12 and takes target body to come off carefully
Born of the same parents；Finally, probe 4 is removed, encapsulation preserves censorship, and DNA inspections can directly be carried out by having ready conditions, can be by for convenience of examining during inspection
Rotating shaft 11 fractures, and takes the part of syringe needle 12 to test.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (8)
1. human body extraction device of fallen cells under a kind of mirror, it is characterised in that：Including microscope (1), universal displacement connector (2)
With extract probe (4), the extraction probe (4) includes needle handle (10) and syringe needle (12), is attached with the syringe needle (12) organic
Bonding agent, one end of the needle handle (10) is rotatably connected to rotating shaft (11), the one of the remote needle handle (10) of the rotating shaft (11)
End is fixedly connected with the syringe needle (12), and one end of the universal displacement connector (2) is fixedly connected on the microscope (1)
On support, the other end is fixedly installed connector (3), and the needle handle (10) is removably connected with the connector (3), described
Extract probe (4) can be extended with the displacement of the universal displacement connector (2) microscope (1) object lens and objective table it
Between, the fixing device for fixing object is provided with the objective table of the microscope (1)；
The fixing device includes pressure ring (5), two pieces of risers (7) and air bag (15), and the objective table level of the microscope (1) is set
Put, the pressure ring (5) is removably horizontally set on the objective table upper surface of the microscope (1)；Two pieces of risers (7) point
The objective table upper surface for being arranged on the microscope (1), the both sides of the pressure ring (5) are not relatively fixed vertically, through two pieces of institutes
State riser (7) and be threaded with horizontally disposed clamping screw (8) respectively, two positive and negative twist of clamping screw (8) can phase
Mutually draw close or remote, one end that two clamping screws (8) are mutually drawn close is fixedly installed three pin holders (6) respectively；Institute
State the objective table upper surface that air bag (15) is fixedly installed on the microscope (1).
2. human body extraction device of fallen cells under a kind of mirror according to claim 1, it is characterised in that：The connector
(3) it is the connecting rod of Band clamp, the needle handle (10) is clamped by the clip of the connecting rod.
3. human body extraction device of fallen cells under a kind of mirror according to claim 2, it is characterised in that：The needle handle (10)
The clip of the upper correspondence connecting rod offers clip slot (9), and the clip of the connecting rod sandwiches the clip slot (9) to clamp the needle handle
(10)。
4. human body extraction device of fallen cells under a kind of mirror according to any one of claims 1 to 3, it is characterised in that：Institute
State syringe needle (12) to be made up of glass fibre, and circumferentially offer thread groove (13) along its length.
5. human body extraction device of fallen cells under a kind of mirror according to any one of claims 1 to 3, it is characterised in that：Institute
Rotating shaft (11) is stated for breakable rotating shaft.
6. human body extraction device of fallen cells under a kind of mirror according to any one of claims 1 to 3, it is characterised in that：Institute
Organic adhesive is stated for epoxide-resin glue.
7. human body extraction device of fallen cells under a kind of mirror according to any one of claims 1 to 3, it is characterised in that：Institute
Universal displacement connector (2) is stated for Universal cantilever.
8. human body extraction device of fallen cells under a kind of mirror according to claim 1, it is characterised in that：The pressure ring (5)
For rectangle pressure ring, and removably it is connected with the objective table upper surface of the microscope (1) by many screws (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511026942.8A CN105400687B (en) | 2015-12-30 | 2015-12-30 | Human body extraction device of fallen cells under a kind of mirror |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511026942.8A CN105400687B (en) | 2015-12-30 | 2015-12-30 | Human body extraction device of fallen cells under a kind of mirror |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105400687A CN105400687A (en) | 2016-03-16 |
| CN105400687B true CN105400687B (en) | 2017-09-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201511026942.8A Expired - Fee Related CN105400687B (en) | 2015-12-30 | 2015-12-30 | Human body extraction device of fallen cells under a kind of mirror |
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| CN (1) | CN105400687B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105713829B (en) * | 2016-03-18 | 2019-03-05 | 中国科学院长春应用化学研究所 | A kind of unicellular sampler and sampling method based on porous metals enrichment probe |
| CN107272179A (en) * | 2017-07-05 | 2017-10-20 | 全讯射频科技(无锡)有限公司 | A kind of microscope carrier |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205258471U (en) * | 2015-12-30 | 2016-05-25 | 张鹏 | Human body cell extraction device that comes off under mirror |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130072934A1 (en) * | 2011-09-19 | 2013-03-21 | Mehmet Aygun | Bone cutter with vacuum or impulsive system |
| CN202322807U (en) * | 2011-11-18 | 2012-07-11 | 彭建军 | Microscopic injection needle mounting module and needle holder assembly |
| CN203849495U (en) * | 2014-03-07 | 2014-09-24 | 中国水产科学研究院南海水产研究所热带水产研究开发中心 | Optical microscope operation needle |
| CN203794897U (en) * | 2014-04-16 | 2014-08-27 | 牛一平 | Cast-off cell adhering device |
| CN204394580U (en) * | 2014-11-19 | 2015-06-17 | 江苏健友医疗科技有限公司 | Single use steril cell harvester |
| CN104630050B (en) * | 2015-01-19 | 2017-06-27 | 中国科学院物理研究所 | A kind of cell injection and the micro-nano suction tube device for extracting |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205258471U (en) * | 2015-12-30 | 2016-05-25 | 张鹏 | Human body cell extraction device that comes off under mirror |
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| CN105400687A (en) | 2016-03-16 |
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Inventor after: Xia Zepei Inventor after: Yin Yu Inventor after: Zhang Peng Inventor after: Xu Yidun Inventor after: Cao Kun Inventor before: Zhang Peng Inventor before: Xia Zepei Inventor before: Xu Yidun Inventor before: Yin Yu Inventor before: Cao Kun |
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Address after: 408400 Chongqing Nanchuan District School Road No. 7 West Street Office Patentee after: Zhang Peng Address before: 408400 west city of Chongqing Nanchuan District Yuzhong Street Office School Road No. 7 Patentee before: Zhang Peng |
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