CN105400587A - Biological enzyme method for extracting fish oil from leftovers of sardine - Google Patents
Biological enzyme method for extracting fish oil from leftovers of sardine Download PDFInfo
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- CN105400587A CN105400587A CN201511015430.1A CN201511015430A CN105400587A CN 105400587 A CN105400587 A CN 105400587A CN 201511015430 A CN201511015430 A CN 201511015430A CN 105400587 A CN105400587 A CN 105400587A
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- tankage
- fish oil
- sardines
- biological enzyme
- extracts
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
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- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
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- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention provides a biological enzyme method for extracting fish oil from leftovers of sardine. The method comprises the following steps: adjusting the pH value of the leftovers of sardine; carrying out enzymatic hydrolysis with alkali protease; then carrying out heating to kill enzyme; and carrying out centrifugation to allow enzymatic hydrolysis liquid to be divided into three layers and sucking up fish oil at the upper layer. With the method, the enzymatic hydrolysis liquid can be fully utilized in the process of preparation of the fish oil, e.g., products like functional polypeptide and flavorings for marine products can be produced; so protein and grease in the leftovers of sardine are fully utilized, the added value of the industry of sardine is increased, and environmental pollution is mitigated. The method has mild reaction conditions and low cost, is simple to operate and can be extensively applied to industrial production.
Description
Technical field
The present invention relates to fishery product deep process technology field, be specifically related to a kind of biological enzyme extracts fish oil method from sardines tankage.
Background technology
Fish oil is rich in the polyunsaturated fatty acids such as EPA, DHA; there is multiple physiologically active as strengthened nervous function, brain healthy, prevention senile dementia; suppress platelet aggregation, reduce thrombosis, prevent cardiovascular and cerebrovascular diseases; anti-inflammatory, anticancer, enhancing autoimmunity, vision protection etc.Some commodity based on fish oil nutrition and pharmaceutical use increase just gradually, and the extraction exploitation of fish oil has become the focus of food resource exploitation.
The production method of fish oil mainly contains milling process, light alkali hydrolysis method, solvent method, water law, enzymolysis process etc.Wherein, milling process is mainly used in the byproduct of reduction plant, and fish oil extraction yield is general lower, second-rate; Light alkali hydrolysis method alkali concn is not easy to control, and not exclusively, too high meeting causes the saponification of fish oil, all can reduce the extraction yield of grease, and the waste water sodium salt content produced in leaching process is high, can not utilize further, forms new waste in too low then hydrolysis; Solvent method adopts organic solvent to extract the fat in raw material, and may there is dissolvent residual in the fish oil of extraction, application is restricted; And Enzymatic Extraction mild condition, low to the destruction of fish oil functional ingredient, the fish oil quality of production is high, can make full use of protease hydrolysis simultaneously and produce enzymolysis solution.
China's southeastern coast has abundant sardines class resource, and sardines has the advantage that growth is fast, reproductivity is strong, and Fresh & Tender in Texture, and fatty height, can be used as the extraction raw material of fish oil.Sardines has very high nutritive value, directly can cook edible or be processed into canned sardines, a large amount of tankage such as fish head, fish tail, internal organ can be produced in the course of processing, how directly to abandon as rubbish, so both waste resource and also can cause detrimentally affect to environment.
Summary of the invention
The object of this invention is to provide a kind of biological enzyme and extract the method for fish oil from sardines tankage, obtain extraction yield and the higher fish oil of fish oil quality, to overcome low, the inferior technical problem of current fish oil extraction yield.
In order to achieve the above object, the technical scheme that the present invention takes is:
Biological enzyme extracts a method for fish oil from sardines tankage, comprises the steps:
(1) after the tankage such as fish head, fish tail, internal organ of sardines being removed foreign material, clean up, mincer rubs, and forms tankage homogenate;
(2) tankage homogenate and water are allocated by certain weight ratio, join in enzymatic vessel, heat temperature raising, add Sumizyme MP, and use acid-alkali accommodation pH, 100 ~ 200r/min at the uniform velocity stirs, and after insulation enzymolysis 1 ~ 6h, enzymolysis solution heats the enzyme that goes out;
(3) enzymolysis solution is used centrifuge while hot, centrifugal rear enzymolysis solution is divided into three layers, is respectively lower floor's enzymolysis solution, the not tolerant and upper strata fish oil in middle level, draws upper strata and namely obtains fish oil.
Preferably, the amount ratio of described tankage homogenate and water is 1:0.5 ~ 2.5.
Preferably, the consumption of described step (2) neutral and alkali proteolytic enzyme with sardines tankage weight for standard adds by 300 ~ 900U/g.
Preferably, 40 ~ 60 DEG C are heated in described step (2).
Preferably, described step (2) is middle is 6.0 ~ 8.0 with acid-alkali accommodation pH.
Preferably, in described step (2), enzymolysis solution is heated to 90 ~ 95 DEG C, and go out enzyme 10 ~ 15min.
Preferably, in described step (3), whizzer is centrifugal with 5000 ~ 8000r/min, centrifugal 10 ~ 15min.
Compared with prior art, beneficial effect of the present invention:
Adopt biological enzyme to extract fish oil extraction yield high, fish oil quality is good, its quality clear, has micro-fishy smell of fish oil, without rancid flavor, EPA and DHA content high; The enzymolysis solution adopting the method to produce in fish oil process can make full use of, as produced the product such as functional polypeptide and marine products food flavouring, protein and grease in sardines tankage are obtained for make full use of, improve the added value of sardines industry and alleviate environmental pollution.The method reaction conditions is gentle, and cost is low, simple to operate, can be widely used in suitability for industrialized production.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
(1) after the tankage such as fish head, fish tail, internal organ of sardines being removed foreign material, clean up, mincer rubs, and forms tankage homogenate;
(2) be that 1:0.5 allocates by the weight ratio of tankage homogenate and water, join in enzymatic vessel, temperature rises to 40 DEG C, with sardines tankage weight for standard adds Sumizyme MP by 300U/g, pH is made to remain on 6.0 with acid-alkali accommodation, 100r/min at the uniform velocity stirs, and after insulation enzymolysis 1h, go out under enzymolysis solution 90 DEG C of conditions enzyme 15min;
(3) enzymolysis solution is used while hot the centrifugal 10min of whizzer 5000r/min, centrifugal rear enzymolysis solution is divided into three layers, is respectively lower floor's enzymolysis solution, the not tolerant and upper strata fish oil in middle level, draws upper strata and namely obtains fish oil.
Embodiment 2
(1) after the tankage such as fish head, fish tail, internal organ of sardines being removed foreign material, clean up, mincer rubs, and forms tankage homogenate;
(2) be that 1:1 allocates by the weight ratio of tankage homogenate and water, join in enzymatic vessel, temperature rises to 45 DEG C, with sardines tankage weight for standard adds Sumizyme MP by 450U/g, pH is made to remain on 6.5 with acid-alkali accommodation, 150r/min at the uniform velocity stirs, and after insulation enzymolysis 2h, go out under enzymolysis solution 92 DEG C of conditions enzyme 16min;
(3) enzymolysis solution is used while hot the centrifugal 12min of whizzer 5500r/min, centrifugal rear enzymolysis solution is divided into three layers, is respectively lower floor's enzymolysis solution, the not tolerant and upper strata fish oil in middle level, draws upper strata and namely obtains fish oil.
Embodiment 3
(1) after the tankage such as fish head, fish tail, internal organ of sardines being removed foreign material, clean up, mincer rubs, and forms tankage homogenate;
(2) be that 1:1.5 allocates by the weight ratio of tankage homogenate and water, join in enzymatic vessel, temperature rises to 50 DEG C, with sardines tankage weight for standard adds Sumizyme MP by 600U/g, pH is made to remain on 7.0 with acid-alkali accommodation, 180r/min at the uniform velocity stirs, and after insulation enzymolysis 3h, go out under enzymolysis solution 93 DEG C of conditions enzyme 18min;
(3) enzymolysis solution is used while hot the centrifugal 13min of whizzer 6000r/min, centrifugal rear enzymolysis solution is divided into three layers, is respectively lower floor's enzymolysis solution, the not tolerant and upper strata fish oil in middle level, draws upper strata and namely obtains fish oil.
Embodiment 4
(1) after the tankage such as fish head, fish tail, internal organ of sardines being removed foreign material, clean up, mincer rubs, and forms tankage homogenate;
(2) be that 1:2 allocates by the weight ratio of tankage homogenate and water, join in enzymatic vessel, temperature rises to 55 DEG C, with sardines tankage weight for standard adds Sumizyme MP by 800U/g, pH is made to remain on 7.5 with acid-alkali accommodation, 190r/min at the uniform velocity stirs, and after insulation enzymolysis 4h, go out under enzymolysis solution 95 DEG C of conditions enzyme 18min;
(3) enzymolysis solution is used while hot the centrifugal 15min of whizzer 7000r/min, centrifugal rear enzymolysis solution is divided into three layers, is respectively lower floor's enzymolysis solution, the not tolerant and upper strata fish oil in middle level, draws upper strata and namely obtains fish oil.
Embodiment 5
(1) after the tankage such as fish head, fish tail, internal organ of sardines being removed foreign material, clean up, mincer rubs, and forms tankage homogenate;
(2) be that 1:2.5 allocates by the weight ratio of tankage homogenate and water, join in enzymatic vessel, temperature rises to 60 DEG C, with sardines tankage weight for standard adds Sumizyme MP by 900U/g, pH is made to remain on 8.0 with acid-alkali accommodation, 200r/min at the uniform velocity stirs, and after insulation enzymolysis 6h, go out under enzymolysis solution 94 DEG C of conditions enzyme 19min;
(3) enzymolysis solution is used while hot the centrifugal 15min of whizzer 8000r/min, centrifugal rear enzymolysis solution is divided into three layers, is respectively lower floor's enzymolysis solution, the not tolerant and upper strata fish oil in middle level, draws upper strata and namely obtains fish oil.
The performance that embodiment 1,2,3,4,5 extracts the fish oil obtained tested, result is as shown in table 1 below.
Table 1
The above, it is only present pre-ferred embodiments, not technical scope of the present invention is imposed any restrictions, thus every above embodiment is done according to technical spirit of the present invention any trickle amendment, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (7)
1. biological enzyme extracts a method for fish oil from sardines tankage, it is characterized in that, comprises the steps:
(1) after the tankage such as fish head, fish tail, internal organ of sardines being removed foreign material, clean up, mincer rubs, and forms tankage homogenate;
(2) tankage homogenate and water are allocated by certain weight ratio, join in enzymatic vessel, heat temperature raising, add Sumizyme MP, and use acid-alkali accommodation pH, 100 ~ 200r/min at the uniform velocity stirs, and after insulation enzymolysis 1 ~ 6h, enzymolysis solution heats the enzyme that goes out;
(3) enzymolysis solution is used centrifuge while hot, centrifugal rear enzymolysis solution is divided into three layers, is respectively lower floor's enzymolysis solution, the not tolerant and upper strata fish oil in middle level, draws upper strata and namely obtains fish oil.
2. biological enzyme according to claim 1 extracts the method for fish oil from sardines tankage, and it is characterized in that, the amount ratio of described tankage homogenate and water is 1:0.5 ~ 2.5.
3. biological enzyme according to claim 1 extracts the method for fish oil from sardines tankage, it is characterized in that, the consumption of described step (2) neutral and alkali proteolytic enzyme with sardines tankage weight for standard adds by 300 ~ 900U/g.
4. biological enzyme according to claim 1 extracts the method for fish oil from sardines tankage, and it is characterized in that, described step is heated to 40 ~ 60 DEG C in (2).
5. biological enzyme according to claim 1 extracts the method for fish oil from sardines tankage, it is characterized in that, described step (2) is middle is 6.0 ~ 8.0 with acid-alkali accommodation pH.
6. biological enzyme according to claim 1 extracts the method for fish oil from sardines tankage, it is characterized in that, in described step (2), enzymolysis solution is heated to 90 ~ 95 DEG C, and go out enzyme 10 ~ 15min.
7. biological enzyme according to claim 1 extracts the method for fish oil from sardines tankage, it is characterized in that, in described step (3), whizzer is centrifugal with 5000 ~ 8000r/min, centrifugal 10 ~ 15min.
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| CN201511015430.1A CN105400587A (en) | 2015-12-29 | 2015-12-29 | Biological enzyme method for extracting fish oil from leftovers of sardine |
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| CN201511015430.1A CN105400587A (en) | 2015-12-29 | 2015-12-29 | Biological enzyme method for extracting fish oil from leftovers of sardine |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107320497A (en) * | 2017-07-24 | 2017-11-07 | 武汉轻工大学 | Purposes of the sturgeon extract oil in lowering fat and protecting liver health food or medicine is prepared |
| CN113502187A (en) * | 2021-07-13 | 2021-10-15 | 满洲里双实肉类食品有限公司 | Method for refining sheep tail oil by biological enzyme method |
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| CN101248821A (en) * | 2007-11-19 | 2008-08-27 | 广东海洋大学 | Process engineering for extracting fishes processing offal fish oil by enzymolysis process |
| CN101768507A (en) * | 2010-01-18 | 2010-07-07 | 广西南宁百洋饲料集团有限公司 | Method for extracting crude fish oil in leftovers of tilapia |
| CN102925279A (en) * | 2012-11-13 | 2013-02-13 | 湖北金鲤鱼食品有限公司 | Technology of extracting and refining fish oil from internal organs of silver carps |
| CN102942987A (en) * | 2012-11-08 | 2013-02-27 | 天津科技大学 | Method for extracting fish oil from head and internal organ of Scomber japonicus by using compound protease |
| CN104194911A (en) * | 2014-08-29 | 2014-12-10 | 上海海洋大学 | Method for refining tilapia oil |
| CN104304647A (en) * | 2014-09-12 | 2015-01-28 | 浙江海洋学院 | Monkfish leftover classification using method |
| CN104342474A (en) * | 2014-10-15 | 2015-02-11 | 浙江海洋学院 | Comprehensive utilization method of tuna leftovers |
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2015
- 2015-12-29 CN CN201511015430.1A patent/CN105400587A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101248821A (en) * | 2007-11-19 | 2008-08-27 | 广东海洋大学 | Process engineering for extracting fishes processing offal fish oil by enzymolysis process |
| CN101768507A (en) * | 2010-01-18 | 2010-07-07 | 广西南宁百洋饲料集团有限公司 | Method for extracting crude fish oil in leftovers of tilapia |
| CN102942987A (en) * | 2012-11-08 | 2013-02-27 | 天津科技大学 | Method for extracting fish oil from head and internal organ of Scomber japonicus by using compound protease |
| CN102925279A (en) * | 2012-11-13 | 2013-02-13 | 湖北金鲤鱼食品有限公司 | Technology of extracting and refining fish oil from internal organs of silver carps |
| CN104194911A (en) * | 2014-08-29 | 2014-12-10 | 上海海洋大学 | Method for refining tilapia oil |
| CN104304647A (en) * | 2014-09-12 | 2015-01-28 | 浙江海洋学院 | Monkfish leftover classification using method |
| CN104342474A (en) * | 2014-10-15 | 2015-02-11 | 浙江海洋学院 | Comprehensive utilization method of tuna leftovers |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107320497A (en) * | 2017-07-24 | 2017-11-07 | 武汉轻工大学 | Purposes of the sturgeon extract oil in lowering fat and protecting liver health food or medicine is prepared |
| CN113502187A (en) * | 2021-07-13 | 2021-10-15 | 满洲里双实肉类食品有限公司 | Method for refining sheep tail oil by biological enzyme method |
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Application publication date: 20160316 |